Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98032.t1 | KEH42179.1 | 91.5 | 188 | 11 | 3 | 1 | 187 | 1 | 184 | 1.90E-86 | 328.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98032.t1 | A0A072VJF3 | 91.5 | 188 | 11 | 3 | 1 | 187 | 1 | 184 | 1.4e-86 | 328.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050670 | MS.gene98032 | 0.849235 | 3.68E-60 | -1.69E-46 |
| MS.gene050747 | MS.gene98032 | 0.824559 | 7.56E-54 | -1.69E-46 |
| MS.gene050950 | MS.gene98032 | 0.815508 | 8.92E-52 | -1.69E-46 |
| MS.gene051387 | MS.gene98032 | 0.838546 | 2.70E-57 | -1.69E-46 |
| MS.gene051525 | MS.gene98032 | 0.849831 | 2.51E-60 | -1.69E-46 |
| MS.gene051588 | MS.gene98032 | 0.872568 | 2.88E-67 | -1.69E-46 |
| MS.gene051838 | MS.gene98032 | 0.813468 | 2.52E-51 | -1.69E-46 |
| MS.gene052414 | MS.gene98032 | 0.856441 | 3.21E-62 | -1.69E-46 |
| MS.gene052421 | MS.gene98032 | 0.815574 | 8.62E-52 | -1.69E-46 |
| MS.gene052501 | MS.gene98032 | 0.833547 | 5.01E-56 | -1.69E-46 |
| MS.gene052505 | MS.gene98032 | 0.843403 | 1.43E-58 | -1.69E-46 |
| MS.gene052982 | MS.gene98032 | 0.836953 | 6.92E-57 | -1.69E-46 |
| MS.gene053169 | MS.gene98032 | 0.815255 | 1.02E-51 | -1.69E-46 |
| MS.gene053873 | MS.gene98032 | 0.823573 | 1.29E-53 | -1.69E-46 |
| MS.gene054102 | MS.gene98032 | 0.8282 | 1.03E-54 | -1.69E-46 |
| MS.gene054719 | MS.gene98032 | 0.802798 | 4.73E-49 | -1.69E-46 |
| MS.gene054920 | MS.gene98032 | 0.845343 | 4.31E-59 | -1.69E-46 |
| MS.gene054927 | MS.gene98032 | 0.818303 | 2.10E-52 | -1.69E-46 |
| MS.gene055369 | MS.gene98032 | 0.850479 | 1.65E-60 | -1.69E-46 |
| MS.gene055539 | MS.gene98032 | 0.842375 | 2.69E-58 | -1.69E-46 |
| MS.gene05553 | MS.gene98032 | 0.811072 | 8.41E-51 | -1.69E-46 |
| MS.gene055741 | MS.gene98032 | 0.847268 | 1.29E-59 | -1.69E-46 |
| MS.gene055744 | MS.gene98032 | 0.851182 | 1.05E-60 | -1.69E-46 |
| MS.gene055911 | MS.gene98032 | 0.804049 | 2.60E-49 | -1.69E-46 |
| MS.gene056040 | MS.gene98032 | 0.853578 | 2.18E-61 | -1.69E-46 |
| MS.gene056166 | MS.gene98032 | 0.845691 | 3.47E-59 | -1.69E-46 |
| MS.gene056514 | MS.gene98032 | 0.88044 | 5.48E-70 | -1.69E-46 |
| MS.gene056877 | MS.gene98032 | 0.816666 | 4.92E-52 | -1.69E-46 |
| MS.gene057019 | MS.gene98032 | 0.812575 | 3.96E-51 | -1.69E-46 |
| MS.gene057124 | MS.gene98032 | 0.803275 | 3.77E-49 | -1.69E-46 |
| MS.gene057605 | MS.gene98032 | -0.801774 | 7.68E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene98032 | 0.817006 | 4.12E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene98032 | 0.818381 | 2.02E-52 | -1.69E-46 |
| MS.gene057825 | MS.gene98032 | 0.816244 | 6.11E-52 | -1.69E-46 |
| MS.gene058007 | MS.gene98032 | 0.828235 | 1.01E-54 | -1.69E-46 |
| MS.gene058120 | MS.gene98032 | 0.807752 | 4.34E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene98032 | 0.818204 | 2.21E-52 | -1.69E-46 |
| MS.gene058444 | MS.gene98032 | 0.853192 | 2.81E-61 | -1.69E-46 |
| MS.gene058700 | MS.gene98032 | 0.885749 | 6.22E-72 | -1.69E-46 |
| MS.gene058948 | MS.gene98032 | 0.804755 | 1.86E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene98032 | 0.813736 | 2.20E-51 | -1.69E-46 |
| MS.gene059455 | MS.gene98032 | 0.829296 | 5.57E-55 | -1.69E-46 |
| MS.gene059684 | MS.gene98032 | 0.800289 | 1.55E-48 | -1.69E-46 |
| MS.gene059868 | MS.gene98032 | 0.812968 | 3.25E-51 | -1.69E-46 |
| MS.gene059870 | MS.gene98032 | 0.809838 | 1.55E-50 | -1.69E-46 |
| MS.gene060372 | MS.gene98032 | 0.839177 | 1.85E-57 | -1.69E-46 |
| MS.gene060403 | MS.gene98032 | 0.80767 | 4.51E-50 | -1.69E-46 |
| MS.gene060485 | MS.gene98032 | 0.825658 | 4.16E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene98032 | 0.811126 | 8.18E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98032.t1 | MTR_1g063100 | 93.617 | 188 | 7 | 3 | 1 | 187 | 1 | 184 | 1.14e-124 | 349 |
| MS.gene98032.t1 | MTR_1g063100 | 92.553 | 188 | 9 | 3 | 1 | 187 | 1 | 184 | 2.74e-122 | 345 |
| MS.gene98032.t1 | MTR_1g063100 | 92.973 | 185 | 8 | 3 | 1 | 184 | 1 | 181 | 4.97e-121 | 340 |
| MS.gene98032.t1 | MTR_1g063100 | 92.973 | 185 | 8 | 3 | 1 | 184 | 1 | 181 | 1.12e-120 | 342 |
| MS.gene98032.t1 | MTR_1g063100 | 97.059 | 102 | 3 | 0 | 85 | 186 | 39 | 140 | 4.27e-70 | 211 |
| MS.gene98032.t1 | MTR_1g063100 | 97.000 | 100 | 3 | 0 | 85 | 184 | 39 | 138 | 1.15e-68 | 208 |
| MS.gene98032.t1 | MTR_6g035275 | 96.491 | 57 | 2 | 0 | 131 | 187 | 1 | 57 | 2.94e-37 | 123 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98032.t1 | AT2G36895 | 61.780 | 191 | 57 | 4 | 1 | 187 | 1 | 179 | 1.94e-78 | 234 |
| MS.gene98032.t1 | AT2G36895 | 61.458 | 192 | 57 | 5 | 1 | 187 | 1 | 180 | 8.60e-77 | 229 |
| MS.gene98032.t1 | AT2G36895 | 53.881 | 219 | 57 | 6 | 1 | 187 | 1 | 207 | 1.41e-70 | 215 |
Find 0 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTTTACTTAAAATAAAA+TGG | + | 10965:21677-21696 | MS.gene98032:intron | 10.0% |
| !!! | TTATTTTAAGTAAAGATTTT+GGG | - | 10965:21675-21694 | None:intergenic | 10.0% |
| !!! | TTTATTTTAAGTAAAGATTT+TGG | - | 10965:21676-21695 | None:intergenic | 10.0% |
| !!! | ATGTTGAAATTTAACTTTAT+TGG | + | 10965:21813-21832 | MS.gene98032:intron | 15.0% |
| !!! | TAAATTTCAACATATGTTTT+GGG | - | 10965:21806-21825 | None:intergenic | 15.0% |
| !!! | TGCTTTTATAATTTAAAGAA+TGG | + | 10965:21255-21274 | MS.gene98032:intron | 15.0% |
| !!! | TTAAATTTCAACATATGTTT+TGG | - | 10965:21807-21826 | None:intergenic | 15.0% |
| !! | ACATAAAAAAAACTATCAGT+AGG | - | 10965:21586-21605 | None:intergenic | 20.0% |
| !! | TTAAAAACTTAAGCTGTTAT+TGG | + | 10965:21889-21908 | MS.gene98032:intron | 20.0% |
| !! | TTTCTTCAATTGAATACAAA+AGG | - | 10965:21772-21791 | None:intergenic | 20.0% |
| !!! | AATGGATTTAAACAATAAGA+TGG | - | 10965:22094-22113 | None:intergenic | 20.0% |
| ! | AAACTGCTAAAAAATATCAG+CGG | + | 10965:22182-22201 | MS.gene98032:CDS | 25.0% |
| ! | ACACGTTAAATTCAAAGATT+AGG | + | 10965:20948-20967 | MS.gene98032:intron | 25.0% |
| ! | CAAAATCAAGAAAAGAAATC+AGG | - | 10965:20922-20941 | None:intergenic | 25.0% |
| ! | CACGTTAAATTCAAAGATTA+GGG | + | 10965:20949-20968 | MS.gene98032:intron | 25.0% |
| ! | TAATCTTTGAATTTAACGTG+TGG | - | 10965:20949-20968 | None:intergenic | 25.0% |
| !! | AGATTTATTTGCGTTTTTCA+TGG | + | 10965:21370-21389 | MS.gene98032:intron | 25.0% |
| !! | TATCTGAAAGTCTGAATTTT+GGG | + | 10965:21320-21339 | MS.gene98032:intron | 25.0% |
| !! | TATTATGTACTCGTTCTTTT+TGG | + | 10965:21923-21942 | MS.gene98032:intron | 25.0% |
| !! | TTATCTGAAAGTCTGAATTT+TGG | + | 10965:21319-21338 | MS.gene98032:intron | 25.0% |
| !!! | ATTAATTTGCATGTATAGTG+TGG | + | 10965:21020-21039 | MS.gene98032:intron | 25.0% |
| !!! | CTGATATTTTTTAGCAGTTT+GGG | - | 10965:22182-22201 | None:intergenic | 25.0% |
| !!! | GAATCAGCTTTTAACTTTTA+AGG | + | 10965:22016-22035 | MS.gene98032:intron | 25.0% |
| !!! | TCAGATAAAATCGTTTTAAC+TGG | - | 10965:21307-21326 | None:intergenic | 25.0% |
| !!! | TTCTTTTAGATTAGCTAAGT+AGG | + | 10965:22127-22146 | MS.gene98032:intron | 25.0% |
| AAGAATCTCTGACATTGAAA+TGG | - | 10965:22112-22131 | None:intergenic | 30.0% | |
| AAGAGAATCTGAATCTGAAT+AGG | - | 10965:20682-20701 | None:intergenic | 30.0% | |
| AATGAGAAAGATTTGGAAGA+AGG | - | 10965:20734-20753 | None:intergenic | 30.0% | |
| AATTGAATACAAAAGGAGCT+TGG | - | 10965:21765-21784 | None:intergenic | 30.0% | |
| AATTTGAATTTCCTCTTTCC+AGG | + | 10965:22338-22357 | MS.gene98032:intron | 30.0% | |
| ACTTCTCACAGAAATTCAAT+GGG | + | 10965:21446-21465 | MS.gene98032:intron | 30.0% | |
| AGAGAATCTGAATCTGAATA+GGG | - | 10965:20681-20700 | None:intergenic | 30.0% | |
| AGTCTTTCATGTAGAACAAT+TGG | + | 10965:21981-22000 | MS.gene98032:intron | 30.0% | |
| ATTTGAATTTCCTCTTTCCA+GGG | + | 10965:22339-22358 | MS.gene98032:intron | 30.0% | |
| GAATTGATGGAATTGTTGAA+TGG | - | 10965:20710-20729 | None:intergenic | 30.0% | |
| GTTGTTGAATGAGAAAGATT+TGG | - | 10965:20741-20760 | None:intergenic | 30.0% | |
| TTGTTCTACATGAAAGACTA+AGG | - | 10965:21980-21999 | None:intergenic | 30.0% | |
| ! | AAAAAGTGCATCAAATGAGA+AGG | - | 10965:21175-21194 | None:intergenic | 30.0% |
| ! | AGCTGTTATTGGTGATTATA+AGG | + | 10965:21900-21919 | MS.gene98032:intron | 30.0% |
| ! | ATCTGAAAGTCTGAATTTTG+GGG | + | 10965:21321-21340 | MS.gene98032:intron | 30.0% |
| !!! | GCTGATATTTTTTAGCAGTT+TGG | - | 10965:22183-22202 | None:intergenic | 30.0% |
| ACAATAAAAATCTCAGCAGC+TGG | - | 10965:21710-21729 | None:intergenic | 35.0% | |
| ACTGTTGCAATGTAGCAAAA+AGG | - | 10965:21628-21647 | None:intergenic | 35.0% | |
| CAGCAAACGATAACTCATAA+AGG | - | 10965:22311-22330 | None:intergenic | 35.0% | |
| GACTTCTCACAGAAATTCAA+TGG | + | 10965:21445-21464 | MS.gene98032:intron | 35.0% | |
| TTGATGGAATTGTTGAATGG+TGG | - | 10965:20707-20726 | None:intergenic | 35.0% | |
| TTGTTGCAAAAAACTGCTTG+AGG | + | 10965:21207-21226 | MS.gene98032:intron | 35.0% | |
| TTTGGAAGAAGGAGAATTGA+TGG | - | 10965:20723-20742 | None:intergenic | 35.0% | |
| ! | AGAAATTCAATGGGTTTCTC+AGG | + | 10965:21455-21474 | MS.gene98032:intron | 35.0% |
| ! | TGCAACAGTTATCTTTTGTC+AGG | + | 10965:21639-21658 | MS.gene98032:intron | 35.0% |
| !! | GAATTTTGGGGAAATTGTGT+TGG | + | 10965:21333-21352 | MS.gene98032:intron | 35.0% |
| AACTACATGGATCCCAATCA+AGG | + | 10965:22217-22236 | MS.gene98032:CDS | 40.0% | |
| ATTCCTTCGAATTCTTCCCT+TGG | + | 10965:20855-20874 | MS.gene98032:CDS | 40.0% | |
| CTCATGTTTCACAGAAGCAA+GGG | + | 10965:22152-22171 | MS.gene98032:intron | 40.0% | |
| GATTGAGATGCTTAGTGATG+TGG | + | 10965:22407-22426 | MS.gene98032:CDS | 40.0% | |
| GTATCAAGAGAGCAACTACA+TGG | + | 10965:22204-22223 | MS.gene98032:CDS | 40.0% | |
| TCTCATGTTTCACAGAAGCA+AGG | + | 10965:22151-22170 | MS.gene98032:intron | 40.0% | |
| TCTTTCCAGGGACTTGATAT+GGG | + | 10965:22351-22370 | MS.gene98032:intron | 40.0% | |
| TTTACCTTCTTCACCACTGA+CGG | + | 10965:21483-21502 | MS.gene98032:CDS | 40.0% | |
| ! | ACAATATCAAAGCATCCGAG+TGG | - | 10965:21851-21870 | None:intergenic | 40.0% |
| ! | GAAGGAATCGTTGTTGTTGT+TGG | - | 10965:20843-20862 | None:intergenic | 40.0% |
| !! | TTGATGCAGAAGCTGTTTTG+TGG | + | 10965:21079-21098 | MS.gene98032:intron | 40.0% |
| ACCTAGACAAAGGCCTTGAT+TGG | - | 10965:22233-22252 | None:intergenic | 45.0% | |
| ATACTGTCTTGGAGCATGGT+GGG | + | 10965:22436-22455 | MS.gene98032:CDS | 45.0% | |
| CAAGATACTGTCTTGGAGCA+TGG | + | 10965:22432-22451 | MS.gene98032:CDS | 45.0% | |
| CACTAAGCATCTCAATCGAG+CGG | - | 10965:22404-22423 | None:intergenic | 45.0% | |
| CATATCAAGTCCCTGGAAAG+AGG | - | 10965:22352-22371 | None:intergenic | 45.0% | |
| CCTAGACAAAGGCCTTGATT+GGG | - | 10965:22232-22251 | None:intergenic | 45.0% | |
| CTCTTTCCAGGGACTTGATA+TGG | + | 10965:22350-22369 | MS.gene98032:intron | 45.0% | |
| CTTCGAATTCTTCCCTTGGT+CGG | + | 10965:20859-20878 | MS.gene98032:CDS | 45.0% | |
| GAGAGGTAGAAGGAGAAACA+TGG | - | 10965:20775-20794 | None:intergenic | 45.0% | |
| TGTGGTGCAAGATACTGTCT+TGG | + | 10965:22425-22444 | MS.gene98032:CDS | 45.0% | |
| ! | AGGATGAATGAGTCGCTTTG+GGG | - | 10965:20820-20839 | None:intergenic | 45.0% |
| ! | CAAAGAGTGCACCTAGACAA+AGG | - | 10965:22243-22262 | None:intergenic | 45.0% |
| ! | GAGGATGAATGAGTCGCTTT+GGG | - | 10965:20821-20840 | None:intergenic | 45.0% |
| ! | GGAATCGTTGTTGTTGTTGG+AGG | - | 10965:20840-20859 | None:intergenic | 45.0% |
| !! | ACTGACGGACTGATCCAAAT+CGG | + | 10965:21498-21517 | MS.gene98032:CDS | 45.0% |
| ACGAAGAAGACGAGGATGAG+AGG | - | 10965:20792-20811 | None:intergenic | 50.0% | |
| AGACGAGGATGAGAGGTAGA+AGG | - | 10965:20785-20804 | None:intergenic | 50.0% | |
| CCCAATCAAGGCCTTTGTCT+AGG | + | 10965:22229-22248 | MS.gene98032:CDS | 50.0% | |
| CCTTGGTCGGACGATGATAA+CGG | + | 10965:20872-20891 | MS.gene98032:CDS | 50.0% | |
| CGACCAAGGGAAGAATTCGA+AGG | - | 10965:20861-20880 | None:intergenic | 50.0% | |
| CGTTATCATCGTCCGACCAA+GGG | - | 10965:20874-20893 | None:intergenic | 50.0% | |
| GAACCATGTTGCCTCCGATT+TGG | - | 10965:21515-21534 | None:intergenic | 50.0% | |
| GATACTGTCTTGGAGCATGG+TGG | + | 10965:22435-22454 | MS.gene98032:CDS | 50.0% | |
| GATCCAAATCGGAGGCAACA+TGG | + | 10965:21509-21528 | MS.gene98032:CDS | 50.0% | |
| GATTTGGATCAGTCCGTCAG+TGG | - | 10965:21499-21518 | None:intergenic | 50.0% | |
| GGAGGATGAATGAGTCGCTT+TGG | - | 10965:20822-20841 | None:intergenic | 50.0% | |
| TACTGTCTTGGAGCATGGTG+GGG | + | 10965:22437-22456 | MS.gene98032:CDS | 50.0% | |
| ! | GGATGAATGAGTCGCTTTGG+GGG | - | 10965:20819-20838 | None:intergenic | 50.0% |
| ! | GGCAGACGACTCTGCATTTT+TGG | + | 10965:22372-22391 | MS.gene98032:CDS | 50.0% |
| ! | TGGAAGAGTGCGAAACCACT+CGG | + | 10965:21833-21852 | MS.gene98032:intron | 50.0% |
| CAGTCCGTCAGTGGTGAAGA+AGG | - | 10965:21490-21509 | None:intergenic | 55.0% | |
| CCGTTATCATCGTCCGACCA+AGG | - | 10965:20875-20894 | None:intergenic | 55.0% | |
| GGGGAGAAACGAAGAAGACG+AGG | - | 10965:20800-20819 | None:intergenic | 55.0% | |
| GTCTGCCCATATCAAGTCCC+TGG | - | 10965:22359-22378 | None:intergenic | 55.0% | |
| TCAGAAGACTTGACGCGACG+AGG | - | 10965:21537-21556 | None:intergenic | 55.0% | |
| !! | GACGGACTGATCCAAATCGG+AGG | + | 10965:21501-21520 | MS.gene98032:CDS | 55.0% |
| ! | TGTCTTGGAGCATGGTGGGG+AGG | + | 10965:22440-22459 | MS.gene98032:CDS | 60.0% |
| ! | TTGGAGCATGGTGGGGAGGT+AGG | + | 10965:22444-22463 | MS.gene98032:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10965 | gene | 20662 | 22479 | 20662 | ID=MS.gene98032 |
| 10965 | mRNA | 20662 | 22479 | 20662 | ID=MS.gene98032.t1;Parent=MS.gene98032 |
| 10965 | exon | 20662 | 20893 | 20662 | ID=MS.gene98032.t1.exon1;Parent=MS.gene98032.t1 |
| 10965 | CDS | 20662 | 20893 | 20662 | ID=cds.MS.gene98032.t1;Parent=MS.gene98032.t1 |
| 10965 | exon | 21457 | 21557 | 21457 | ID=MS.gene98032.t1.exon2;Parent=MS.gene98032.t1 |
| 10965 | CDS | 21457 | 21557 | 21457 | ID=cds.MS.gene98032.t1;Parent=MS.gene98032.t1 |
| 10965 | exon | 22166 | 22279 | 22166 | ID=MS.gene98032.t1.exon3;Parent=MS.gene98032.t1 |
| 10965 | CDS | 22166 | 22279 | 22166 | ID=cds.MS.gene98032.t1;Parent=MS.gene98032.t1 |
| 10965 | exon | 22360 | 22479 | 22360 | ID=MS.gene98032.t1.exon4;Parent=MS.gene98032.t1 |
| 10965 | CDS | 22360 | 22479 | 22360 | ID=cds.MS.gene98032.t1;Parent=MS.gene98032.t1 |
| Gene Sequence |
| Protein sequence |
