AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060478


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060478.t1 MTR_8g024250 93.950 281 5 1 1 269 1 281 0.0 536
MS.gene060478.t1 MTR_7g118270 63.784 185 67 0 84 268 44 228 4.42e-89 264
MS.gene060478.t1 MTR_7g106050 37.013 154 97 0 90 243 24 177 7.49e-30 112
MS.gene060478.t1 MTR_4g051880 34.507 142 85 1 110 243 45 186 4.10e-22 91.7
MS.gene060478.t1 MTR_3g092790 33.333 150 99 1 109 258 49 197 6.37e-22 90.9
MS.gene060478.t1 MTR_2g086790 30.233 172 107 3 84 243 17 187 1.76e-20 87.4
MS.gene060478.t1 MTR_1g057420 30.464 151 105 0 105 255 39 189 2.51e-20 86.7
MS.gene060478.t1 MTR_2g011350 25.949 158 113 3 97 252 35 190 3.23e-15 72.8
MS.gene060478.t1 MTR_5g087760 26.705 176 117 6 83 247 60 234 9.71e-13 66.6
MS.gene060478.t1 MTR_4g119428 25.658 152 109 3 96 245 65 214 6.29e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060478.t1 AT4G01410 49.462 186 94 0 83 268 41 226 7.97e-57 182
MS.gene060478.t1 AT3G44220 29.609 179 123 1 93 268 27 205 9.78e-25 98.6
MS.gene060478.t1 AT3G52470 30.058 173 119 2 84 255 17 188 9.82e-25 98.6
MS.gene060478.t1 AT5G53730 33.557 149 97 1 97 243 38 186 1.80e-24 97.8
MS.gene060478.t1 AT2G35960 30.968 155 107 0 101 255 36 190 9.63e-24 95.9
MS.gene060478.t1 AT5G22200 32.692 156 105 0 105 260 43 198 3.07e-23 94.7
MS.gene060478.t1 AT5G06330 35.220 159 93 7 105 257 39 193 4.00e-23 94.4
MS.gene060478.t1 AT3G11660 33.735 166 101 7 83 243 16 177 9.84e-23 93.2
MS.gene060478.t1 AT4G09590 29.814 161 113 0 87 247 23 183 1.02e-22 93.2
MS.gene060478.t1 AT2G35970 30.435 161 112 0 87 247 23 183 3.51e-22 91.7
MS.gene060478.t1 AT5G22870 27.933 179 122 2 72 244 9 186 8.22e-17 77.4
MS.gene060478.t1 AT4G05220 24.405 168 125 2 87 254 47 212 7.92e-16 75.1
MS.gene060478.t1 AT1G61760 27.374 179 127 2 87 264 45 221 1.91e-15 73.9
MS.gene060478.t1 AT2G35980 27.848 158 113 1 90 246 45 202 3.86e-13 67.4
MS.gene060478.t1 AT5G06320 29.787 188 117 6 88 268 51 230 5.88e-11 61.2

Find 69 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACGTGGTTGTTCCATTTC+AGG 0.182704 8.1:-76036190 None:intergenic
GTGGGTCAGATTCCTCTTAT+TGG 0.186108 8.1:+76036838 MS.gene060478:CDS
TGTCGTGGTTGTTCCATTTC+GGG 0.198552 8.1:-76036232 None:intergenic
GCATTATAATCCTCATCTTA+TGG 0.245398 8.1:+76036371 MS.gene060478:CDS
TTGTCGTGGTTGTTCCATTT+CGG 0.261861 8.1:-76036233 None:intergenic
CATATGCAGTGTTCACGTTT+TGG 0.295207 8.1:-76036322 None:intergenic
TTCAAAACATCCACCGAAAC+TGG 0.309717 8.1:-76036667 None:intergenic
TTGGTGTTGGATGAGAATTA+TGG 0.323767 8.1:+76036694 MS.gene060478:CDS
TGGTGTTGGATGAGAATTAT+GGG 0.333006 8.1:+76036695 MS.gene060478:CDS
TAAATGGATACACGTTTGTT+TGG 0.340333 8.1:-76036514 None:intergenic
GGAAAGCCTGTCAAAGTAAA+TGG 0.359178 8.1:-76036530 None:intergenic
TGTGAATGGGGTTGTTGGTT+AGG 0.359389 8.1:-76036118 None:intergenic
TATGCTTCCATCGCTTTACT+TGG 0.363370 8.1:+76036594 MS.gene060478:CDS
AGGCGTGCCGGTTCCAGTTT+CGG 0.370573 8.1:+76036654 MS.gene060478:CDS
GTCACAAGTGCTGCAATCTA+TGG 0.378150 8.1:+76036427 MS.gene060478:CDS
ACAATCTTCTTCCTCGTACT+TGG 0.399240 8.1:+76036349 MS.gene060478:CDS
AGCATATGATGAGGCGTAAT+CGG 0.403211 8.1:-76036577 None:intergenic
ACCGGCACGCCTCCTAAAAC+TGG 0.405172 8.1:-76036643 None:intergenic
GCCAAGTGTGATTTATTGTT+GGG 0.416075 8.1:+76036799 MS.gene060478:CDS
TGCAATTTACAATCTTCATA+AGG 0.420084 8.1:+76036488 MS.gene060478:CDS
GGCAGGTTGAGGTGGAAAAC+TGG 0.421371 8.1:+76036745 MS.gene060478:CDS
GGATGAGAATTATGGGGTGG+TGG 0.422334 8.1:+76036702 MS.gene060478:CDS
AACCATACCTTTGTGTTCCA+TGG 0.429303 8.1:-76036275 None:intergenic
TGCCAAGTGTGATTTATTGT+TGG 0.432416 8.1:+76036798 MS.gene060478:CDS
ACGTCGTCAATACCCTGAAA+TGG 0.435785 8.1:+76036177 MS.gene060478:CDS
GTGTATCCATTTACTTTGAC+AGG 0.438624 8.1:+76036524 MS.gene060478:CDS
CATGTATCCACATCACAAAC+TGG 0.441248 8.1:-76036865 None:intergenic
TTATTGTTGGGTTTGAAGAA+AGG 0.446899 8.1:+76036811 MS.gene060478:CDS
GACGTGGTTGTTCCATTTCA+GGG 0.447247 8.1:-76036189 None:intergenic
GATGAGAATTATGGGGTGGT+GGG 0.453278 8.1:+76036703 MS.gene060478:CDS
ACGTCATCAATATCCCGAAA+TGG 0.462255 8.1:+76036219 MS.gene060478:CDS
GATGTTGTGAATGGGGTTGT+TGG 0.473428 8.1:-76036123 None:intergenic
TGAAACTTATATTTCAAGGC+AGG 0.482813 8.1:+76036728 MS.gene060478:CDS
GGTGTGAAACTTATATTTCA+AGG 0.486868 8.1:+76036724 MS.gene060478:CDS
CAAACTGGAGCACCAATAAG+AGG 0.488904 8.1:-76036850 None:intergenic
ACATCCACCGAAACTGGAAC+CGG 0.491068 8.1:-76036661 None:intergenic
TGCATTGAGATTGACATGTC+TGG 0.499930 8.1:-76036469 None:intergenic
GGTTTGAAGAAAGGTCTTGT+GGG 0.505112 8.1:+76036820 MS.gene060478:CDS
TTATATTTCAAGGCAGGTTG+AGG 0.505575 8.1:+76036734 MS.gene060478:CDS
AGGGAAATGATGTTGTGAAT+GGG 0.518707 8.1:-76036131 None:intergenic
TAGGGAAATGATGTTGTGAA+TGG 0.522309 8.1:-76036132 None:intergenic
ACCATACCTTTGTGTTCCAT+GGG 0.526647 8.1:-76036274 None:intergenic
GGGTTTGAAGAAAGGTCTTG+TGG 0.531873 8.1:+76036819 MS.gene060478:CDS
TGACGACGTTGTTGTTGTTG+TGG 0.538741 8.1:-76036163 None:intergenic
TCCCATGGAACACAAAGGTA+TGG 0.547214 8.1:+76036273 MS.gene060478:CDS
GGTGTTGGATGAGAATTATG+GGG 0.556537 8.1:+76036696 MS.gene060478:CDS
TATTTCAAGGCAGGTTGAGG+TGG 0.558650 8.1:+76036737 MS.gene060478:CDS
TGCTTCTCCAAGTAAAGCGA+TGG 0.561546 8.1:-76036601 None:intergenic
TGGTGCTCCAGTTTGTGATG+TGG 0.562878 8.1:+76036858 MS.gene060478:CDS
GGGAAATGATGTTGTGAATG+GGG 0.563425 8.1:-76036130 None:intergenic
ACCCAACAATAAATCACACT+TGG 0.575443 8.1:-76036800 None:intergenic
TGATGTCGTTGTTGTTGTCG+TGG 0.587065 8.1:-76036247 None:intergenic
TATGATGAGGCGTAATCGGA+TGG 0.604145 8.1:-76036573 None:intergenic
CGTGCCGGTTCCAGTTTCGG+TGG 0.607622 8.1:+76036657 MS.gene060478:CDS
ACAACGACATCAATATCCCA+TGG 0.615164 8.1:+76036258 MS.gene060478:CDS
GCACTTGTGACTGTGATGTG+AGG 0.630907 8.1:-76036415 None:intergenic
TGATGACGTTGTTGTTGACG+TGG 0.649666 8.1:-76036205 None:intergenic
GGTATGCAAGCCATAAGATG+AGG 0.669740 8.1:-76036381 None:intergenic
ATGGTTTCTATATGACACAA+AGG 0.671270 8.1:-76036554 None:intergenic
AGCGATGGAAGCATATGATG+AGG 0.674282 8.1:-76036586 None:intergenic
GGATTATAATGCCAAGTACG+AGG 0.674453 8.1:-76036360 None:intergenic
CAATATCCCATGGAACACAA+AGG 0.680881 8.1:+76036268 MS.gene060478:CDS
GTTGGATGAGAATTATGGGG+TGG 0.688698 8.1:+76036699 MS.gene060478:CDS
GGAGAAGCATGAAACCGTGT+CGG 0.692283 8.1:+76036615 MS.gene060478:CDS
TAAAACTGGTGATACCGACA+CGG 0.699605 8.1:-76036629 None:intergenic
ATTGAGATTGACATGTCTGG+TGG 0.701504 8.1:-76036466 None:intergenic
GTTTCTATATGACACAAAGG+CGG 0.718743 8.1:-76036551 None:intergenic
GCATTAGGTACTCTATAACG+AGG 0.741839 8.1:-76036298 None:intergenic
CTGGTGGTGAAGTTGCATTG+AGG 0.741850 8.1:-76036450 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGCAATTTACAATCTTCATA+AGG + chr8.1:76036488-76036507 MS.gene060478:CDS 25.0%
ATGGTTTCTATATGACACAA+AGG - chr8.1:76036557-76036576 None:intergenic 30.0%
GCATTATAATCCTCATCTTA+TGG + chr8.1:76036371-76036390 MS.gene060478:CDS 30.0%
GGTGTGAAACTTATATTTCA+AGG + chr8.1:76036724-76036743 MS.gene060478:CDS 30.0%
TAAATGGATACACGTTTGTT+TGG - chr8.1:76036517-76036536 None:intergenic 30.0%
TGAAACTTATATTTCAAGGC+AGG + chr8.1:76036728-76036747 MS.gene060478:CDS 30.0%
TTATTGTTGGGTTTGAAGAA+AGG + chr8.1:76036811-76036830 MS.gene060478:CDS 30.0%
ACCCAACAATAAATCACACT+TGG - chr8.1:76036803-76036822 None:intergenic 35.0%
AGGGAAATGATGTTGTGAAT+GGG - chr8.1:76036134-76036153 None:intergenic 35.0%
GCCAAGTGTGATTTATTGTT+GGG + chr8.1:76036799-76036818 MS.gene060478:CDS 35.0%
GTGTATCCATTTACTTTGAC+AGG + chr8.1:76036524-76036543 MS.gene060478:CDS 35.0%
GTTTCTATATGACACAAAGG+CGG - chr8.1:76036554-76036573 None:intergenic 35.0%
TAGGGAAATGATGTTGTGAA+TGG - chr8.1:76036135-76036154 None:intergenic 35.0%
TGCCAAGTGTGATTTATTGT+TGG + chr8.1:76036798-76036817 MS.gene060478:CDS 35.0%
TTATATTTCAAGGCAGGTTG+AGG + chr8.1:76036734-76036753 MS.gene060478:CDS 35.0%
! GTTGTTGTGGCATTTTGTTA+GGG - chr8.1:76036153-76036172 None:intergenic 35.0%
! TGGTGTTGGATGAGAATTAT+GGG + chr8.1:76036695-76036714 MS.gene060478:CDS 35.0%
! TGTTGTTGTGGCATTTTGTT+AGG - chr8.1:76036154-76036173 None:intergenic 35.0%
!! TTGGTGTTGGATGAGAATTA+TGG + chr8.1:76036694-76036713 MS.gene060478:CDS 35.0%
!!! TGTTTTGAATGGTTTGGTGT+TGG + chr8.1:76036681-76036700 MS.gene060478:CDS 35.0%
AACCATACCTTTGTGTTCCA+TGG - chr8.1:76036278-76036297 None:intergenic 40.0%
ACAACGACATCAATATCCCA+TGG + chr8.1:76036258-76036277 MS.gene060478:CDS 40.0%
ACAATCTTCTTCCTCGTACT+TGG + chr8.1:76036349-76036368 MS.gene060478:CDS 40.0%
ACCATACCTTTGTGTTCCAT+GGG - chr8.1:76036277-76036296 None:intergenic 40.0%
ACGTCATCAATATCCCGAAA+TGG + chr8.1:76036219-76036238 MS.gene060478:CDS 40.0%
AGCATATGATGAGGCGTAAT+CGG - chr8.1:76036580-76036599 None:intergenic 40.0%
ATTGAGATTGACATGTCTGG+TGG - chr8.1:76036469-76036488 None:intergenic 40.0%
CAATATCCCATGGAACACAA+AGG + chr8.1:76036268-76036287 MS.gene060478:CDS 40.0%
CATGTATCCACATCACAAAC+TGG - chr8.1:76036868-76036887 None:intergenic 40.0%
GCATTAGGTACTCTATAACG+AGG - chr8.1:76036301-76036320 None:intergenic 40.0%
GGAAAGCCTGTCAAAGTAAA+TGG - chr8.1:76036533-76036552 None:intergenic 40.0%
GGATTATAATGCCAAGTACG+AGG - chr8.1:76036363-76036382 None:intergenic 40.0%
GGGAAATGATGTTGTGAATG+GGG - chr8.1:76036133-76036152 None:intergenic 40.0%
TAAAACTGGTGATACCGACA+CGG - chr8.1:76036632-76036651 None:intergenic 40.0%
TATGCTTCCATCGCTTTACT+TGG + chr8.1:76036594-76036613 MS.gene060478:CDS 40.0%
TGCATTGAGATTGACATGTC+TGG - chr8.1:76036472-76036491 None:intergenic 40.0%
TTCAAAACATCCACCGAAAC+TGG - chr8.1:76036670-76036689 None:intergenic 40.0%
TTGTCGTGGTTGTTCCATTT+CGG - chr8.1:76036236-76036255 None:intergenic 40.0%
! CATATGCAGTGTTCACGTTT+TGG - chr8.1:76036325-76036344 None:intergenic 40.0%
! GGTGTTGGATGAGAATTATG+GGG + chr8.1:76036696-76036715 MS.gene060478:CDS 40.0%
! GGTTTGAAGAAAGGTCTTGT+GGG + chr8.1:76036820-76036839 MS.gene060478:CDS 40.0%
!! ACTGTGATGTGAGGTTTTGA+GGG - chr8.1:76036409-76036428 None:intergenic 40.0%
!! GGTGGATGTTTTGAATGGTT+TGG + chr8.1:76036675-76036694 MS.gene060478:CDS 40.0%
!! GTTTCGGTGGATGTTTTGAA+TGG + chr8.1:76036670-76036689 MS.gene060478:CDS 40.0%
!!! TGTTCACGTTTTGGAGCATT+AGG - chr8.1:76036316-76036335 None:intergenic 40.0%
ACGTCGTCAATACCCTGAAA+TGG + chr8.1:76036177-76036196 MS.gene060478:CDS 45.0%
GACGTGGTTGTTCCATTTCA+GGG - chr8.1:76036192-76036211 None:intergenic 45.0%
GATGAGAATTATGGGGTGGT+GGG + chr8.1:76036703-76036722 MS.gene060478:CDS 45.0%
GATGTTGTGAATGGGGTTGT+TGG - chr8.1:76036126-76036145 None:intergenic 45.0%
GGGTTTGAAGAAAGGTCTTG+TGG + chr8.1:76036819-76036838 MS.gene060478:CDS 45.0%
GGTATGCAAGCCATAAGATG+AGG - chr8.1:76036384-76036403 None:intergenic 45.0%
GTGGGTCAGATTCCTCTTAT+TGG + chr8.1:76036838-76036857 MS.gene060478:CDS 45.0%
GTTGGATGAGAATTATGGGG+TGG + chr8.1:76036699-76036718 MS.gene060478:CDS 45.0%
TATGATGAGGCGTAATCGGA+TGG - chr8.1:76036576-76036595 None:intergenic 45.0%
TCCCATGGAACACAAAGGTA+TGG + chr8.1:76036273-76036292 MS.gene060478:CDS 45.0%
TGACGTGGTTGTTCCATTTC+AGG - chr8.1:76036193-76036212 None:intergenic 45.0%
TGCTTCTCCAAGTAAAGCGA+TGG - chr8.1:76036604-76036623 None:intergenic 45.0%
TGTCGTGGTTGTTCCATTTC+GGG - chr8.1:76036235-76036254 None:intergenic 45.0%
! AGCGATGGAAGCATATGATG+AGG - chr8.1:76036589-76036608 None:intergenic 45.0%
! CAAACTGGAGCACCAATAAG+AGG - chr8.1:76036853-76036872 None:intergenic 45.0%
! GACTGTGATGTGAGGTTTTG+AGG - chr8.1:76036410-76036429 None:intergenic 45.0%
! GTCACAAGTGCTGCAATCTA+TGG + chr8.1:76036427-76036446 MS.gene060478:CDS 45.0%
! TATTTCAAGGCAGGTTGAGG+TGG + chr8.1:76036737-76036756 MS.gene060478:CDS 45.0%
! TGACGACGTTGTTGTTGTTG+TGG - chr8.1:76036166-76036185 None:intergenic 45.0%
! TGATGACGTTGTTGTTGACG+TGG - chr8.1:76036208-76036227 None:intergenic 45.0%
! TGATGTCGTTGTTGTTGTCG+TGG - chr8.1:76036250-76036269 None:intergenic 45.0%
! TGTGAATGGGGTTGTTGGTT+AGG - chr8.1:76036121-76036140 None:intergenic 45.0%
!! GTGTCGGTATCACCAGTTTT+AGG + chr8.1:76036631-76036650 MS.gene060478:CDS 45.0%
!!! TCGGTATCACCAGTTTTAGG+AGG + chr8.1:76036634-76036653 MS.gene060478:CDS 45.0%
!!! TGATGTGAGGTTTTGAGGGA+TGG - chr8.1:76036405-76036424 None:intergenic 45.0%
ACATCCACCGAAACTGGAAC+CGG - chr8.1:76036664-76036683 None:intergenic 50.0%
GCACTTGTGACTGTGATGTG+AGG - chr8.1:76036418-76036437 None:intergenic 50.0%
GGATGAGAATTATGGGGTGG+TGG + chr8.1:76036702-76036721 MS.gene060478:CDS 50.0%
TGGTGCTCCAGTTTGTGATG+TGG + chr8.1:76036858-76036877 MS.gene060478:CDS 50.0%
! CTGGTGGTGAAGTTGCATTG+AGG - chr8.1:76036453-76036472 None:intergenic 50.0%
! GGAGAAGCATGAAACCGTGT+CGG + chr8.1:76036615-76036634 MS.gene060478:CDS 50.0%
! GGCAGGTTGAGGTGGAAAAC+TGG + chr8.1:76036745-76036764 MS.gene060478:CDS 55.0%
!!! ACCAGTTTTAGGAGGCGTGC+CGG + chr8.1:76036642-76036661 MS.gene060478:CDS 55.0%
ACCGGCACGCCTCCTAAAAC+TGG - chr8.1:76036646-76036665 None:intergenic 60.0%
!! AGGCGTGCCGGTTCCAGTTT+CGG + chr8.1:76036654-76036673 MS.gene060478:CDS 60.0%
!! CGTGCCGGTTCCAGTTTCGG+TGG + chr8.1:76036657-76036676 MS.gene060478:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 76036079 76036888 76036079 ID=MS.gene060478
chr8.1 mRNA 76036079 76036888 76036079 ID=MS.gene060478.t1;Parent=MS.gene060478
chr8.1 exon 76036079 76036888 76036079 ID=MS.gene060478.t1.exon1;Parent=MS.gene060478.t1
chr8.1 CDS 76036079 76036888 76036079 ID=cds.MS.gene060478.t1;Parent=MS.gene060478.t1
Gene Sequence

>MS.gene060478

ATGGAAGACGAAATAATGCATATAGAACGTCAAGAAGATCCTAACCAACAACCCCATTCACAACATCATTTCCCTAACAAAATGCCACAACAACAACAACGTCGTCAATACCCTGAAATGGAACAACCACGTCAACAACAACGTCATCAATATCCCGAAATGGAACAACCACGACAACAACAACGACATCAATATCCCATGGAACACAAAGGTATGGTTCCTCGTTATAGAGTACCTAATGCTCCAAAACGTGAACACTGCATATGCATCACAATCTTCTTCCTCGTACTTGGCATTATAATCCTCATCTTATGGCTTGCATACCATCCCTCAAAACCTCACATCACAGTCACAAGTGCTGCAATCTATGGCCTCAATGCAACTTCACCACCAGACATGTCAATCTCAATGCAATTTACAATCTTCATAAGGAATCCAAACAAACGTGTATCCATTTACTTTGACAGGCTTTCCGCCTTTGTGTCATATAGAAACCATCCGATTACGCCTCATCATATGCTTCCATCGCTTTACTTGGAGAAGCATGAAACCGTGTCGGTATCACCAGTTTTAGGAGGCGTGCCGGTTCCAGTTTCGGTGGATGTTTTGAATGGTTTGGTGTTGGATGAGAATTATGGGGTGGTGGGTGTGAAACTTATATTTCAAGGCAGGTTGAGGTGGAAAACTGGTGAGATAAAATCTGCACATTATAATATGTATGCCAAGTGTGATTTATTGTTGGGTTTGAAGAAAGGTCTTGTGGGTCAGATTCCTCTTATTGGTGCTCCAGTTTGTGATGTGGATACATGA

Protein sequence

>MS.gene060478.t1

MEDEIMHIERQEDPNQQPHSQHHFPNKMPQQQQRRQYPEMEQPRQQQRHQYPEMEQPRQQQRHQYPMEHKGMVPRYRVPNAPKREHCICITIFFLVLGIIILILWLAYHPSKPHITVTSAAIYGLNATSPPDMSISMQFTIFIRNPNKRVSIYFDRLSAFVSYRNHPITPHHMLPSLYLEKHETVSVSPVLGGVPVPVSVDVLNGLVLDENYGVVGVKLIFQGRLRWKTGEIKSAHYNMYAKCDLLLGLKKGLVGQIPLIGAPVCDVDT