AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08516


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08516.t1 MTR_8g024250 97.173 283 6 1 1 283 1 281 0.0 563
MS.gene08516.t1 MTR_7g118270 63.784 185 67 0 98 282 44 228 3.77e-89 265
MS.gene08516.t1 MTR_7g106050 37.013 154 97 0 104 257 24 177 1.56e-29 111
MS.gene08516.t1 MTR_4g051880 34.507 142 85 1 124 257 45 186 7.47e-22 91.3
MS.gene08516.t1 MTR_3g092790 33.333 150 99 1 123 272 49 197 1.28e-21 90.5
MS.gene08516.t1 MTR_2g086790 30.233 172 107 3 98 257 17 187 3.36e-20 87.0
MS.gene08516.t1 MTR_1g057420 30.464 151 105 0 119 269 39 189 5.51e-20 86.3
MS.gene08516.t1 MTR_2g011350 25.949 158 113 3 111 266 35 190 5.09e-15 72.4
MS.gene08516.t1 MTR_5g087760 26.705 176 117 6 97 261 60 234 1.85e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08516.t1 AT4G01410 49.462 186 94 0 97 282 41 226 1.11e-56 182
MS.gene08516.t1 AT3G44220 29.609 179 123 1 107 282 27 205 2.01e-24 97.8
MS.gene08516.t1 AT3G52470 30.857 175 115 4 98 269 17 188 2.70e-24 97.8
MS.gene08516.t1 AT5G53730 33.557 149 97 1 111 257 38 186 4.41e-24 97.4
MS.gene08516.t1 AT2G35960 30.968 155 107 0 115 269 36 190 2.34e-23 95.1
MS.gene08516.t1 AT5G22200 32.692 156 105 0 119 274 43 198 5.26e-23 94.4
MS.gene08516.t1 AT5G06330 35.220 159 93 7 119 271 39 193 7.69e-23 94.0
MS.gene08516.t1 AT3G11660 33.735 166 101 7 97 257 16 177 1.73e-22 92.8
MS.gene08516.t1 AT4G09590 29.814 161 113 0 101 261 23 183 2.57e-22 92.4
MS.gene08516.t1 AT2G35970 30.435 161 112 0 101 261 23 183 9.57e-22 90.9
MS.gene08516.t1 AT5G22870 27.933 179 122 2 86 258 9 186 1.98e-16 76.6
MS.gene08516.t1 AT4G05220 24.405 168 125 2 101 268 47 212 1.45e-15 74.3
MS.gene08516.t1 AT1G61760 27.374 179 127 2 101 278 45 221 4.74e-15 73.2
MS.gene08516.t1 AT2G35980 27.848 158 113 1 104 260 45 202 8.66e-13 66.6

Find 73 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACGTGGTTGTTCCATTTC+AGG 0.182704 8.3:-68151626 None:intergenic
GTGGGTCAGATTCCTCTTAT+TGG 0.186108 8.3:+68152316 MS.gene08516:CDS
TGTCGTGGTTGTTCCATTTC+GGG 0.198552 8.3:-68151710 None:intergenic
TGACGTGGTTGTTCCATTTC+GGG 0.199740 8.3:-68151668 None:intergenic
GCATTATAATCCTCATCTTA+TGG 0.245398 8.3:+68151849 MS.gene08516:CDS
TTGTCGTGGTTGTTCCATTT+CGG 0.261861 8.3:-68151711 None:intergenic
TTGACGTGGTTGTTCCATTT+CGG 0.262171 8.3:-68151669 None:intergenic
CATATGCAGTGTTCACGTTT+TGG 0.295207 8.3:-68151800 None:intergenic
TTCAAAACATCCACCGAAAC+TGG 0.309717 8.3:-68152145 None:intergenic
TTGGTGTTGGATGAGAATTA+TGG 0.323767 8.3:+68152172 MS.gene08516:CDS
TGGTGTTGGATGAGAATTAT+GGG 0.333006 8.3:+68152173 MS.gene08516:CDS
TAAATGGATACACGTTTGTT+TGG 0.340333 8.3:-68151992 None:intergenic
GGAAAGCCTGTCAAAGTAAA+TGG 0.359178 8.3:-68152008 None:intergenic
TGTGAATGGGGTTGTTGGTT+AGG 0.359389 8.3:-68151554 None:intergenic
TATGCTTCCATCGCTTTACT+TGG 0.363370 8.3:+68152072 MS.gene08516:CDS
AGGCGTGCCGGTTCCAGTTT+CGG 0.370573 8.3:+68152132 MS.gene08516:CDS
GTCACAAGTGCTGCAATCTA+TGG 0.378150 8.3:+68151905 MS.gene08516:CDS
ACAATCTTCTTCCTCGTACT+TGG 0.399240 8.3:+68151827 MS.gene08516:CDS
AGCATATGATGAGGCGTAAT+CGG 0.403211 8.3:-68152055 None:intergenic
ACCGGCACGCCTCCTAAAAC+TGG 0.405172 8.3:-68152121 None:intergenic
GCCAAGTGTGATTTATTGTT+GGG 0.416075 8.3:+68152277 MS.gene08516:CDS
TGCAATTTACAATCTTCATA+AGG 0.420084 8.3:+68151966 MS.gene08516:CDS
GGCAGGTTGAGGTGGAAAAC+TGG 0.421371 8.3:+68152223 MS.gene08516:CDS
GGATGAGAATTATGGGGTGG+TGG 0.422334 8.3:+68152180 MS.gene08516:CDS
AACCATACCTTTGTGTTCCA+TGG 0.429303 8.3:-68151753 None:intergenic
TGCCAAGTGTGATTTATTGT+TGG 0.432416 8.3:+68152276 MS.gene08516:CDS
ACGTCGTCAATACCCTGAAA+TGG 0.435785 8.3:+68151613 MS.gene08516:CDS
GTGTATCCATTTACTTTGAC+AGG 0.438624 8.3:+68152002 MS.gene08516:CDS
TTATTGTTGGGTTTGAAGAA+AGG 0.446899 8.3:+68152289 MS.gene08516:CDS
GACGTGGTTGTTCCATTTCA+GGG 0.447247 8.3:-68151625 None:intergenic
GATGAGAATTATGGGGTGGT+GGG 0.453278 8.3:+68152181 MS.gene08516:CDS
TATGTATCCACATCACAAAC+TGG 0.461285 8.3:-68152343 None:intergenic
ACGTCATCAATATCCCGAAA+TGG 0.462255 8.3:+68151655 MS.gene08516:CDS
ACGTCATCAATATCCCGAAA+TGG 0.462255 8.3:+68151697 MS.gene08516:CDS
GATGTTGTGAATGGGGTTGT+TGG 0.473428 8.3:-68151559 None:intergenic
TGAAACTTATATTTCAAGGC+AGG 0.482813 8.3:+68152206 MS.gene08516:CDS
GGTGTGAAACTTATATTTCA+AGG 0.486868 8.3:+68152202 MS.gene08516:CDS
CAAACTGGAGCACCAATAAG+AGG 0.488904 8.3:-68152328 None:intergenic
ACATCCACCGAAACTGGAAC+CGG 0.491068 8.3:-68152139 None:intergenic
TGCATTGAGATTGACATGTC+TGG 0.499930 8.3:-68151947 None:intergenic
GGTTTGAAGAAAGGTCTTGT+GGG 0.505112 8.3:+68152298 MS.gene08516:CDS
TTATATTTCAAGGCAGGTTG+AGG 0.505575 8.3:+68152212 MS.gene08516:CDS
AGGGAAATGATGTTGTGAAT+GGG 0.518707 8.3:-68151567 None:intergenic
TAGGGAAATGATGTTGTGAA+TGG 0.522309 8.3:-68151568 None:intergenic
ACCATACCTTTGTGTTCCAT+GGG 0.526647 8.3:-68151752 None:intergenic
GGGTTTGAAGAAAGGTCTTG+TGG 0.531873 8.3:+68152297 MS.gene08516:CDS
TGACGACGTTGTTGTTGTTG+TGG 0.538741 8.3:-68151599 None:intergenic
TCCCATGGAACACAAAGGTA+TGG 0.547214 8.3:+68151751 MS.gene08516:CDS
GGTGTTGGATGAGAATTATG+GGG 0.556537 8.3:+68152174 MS.gene08516:CDS
TATTTCAAGGCAGGTTGAGG+TGG 0.558650 8.3:+68152215 MS.gene08516:CDS
TGCTTCTCCAAGTAAAGCGA+TGG 0.561546 8.3:-68152079 None:intergenic
TGGTGCTCCAGTTTGTGATG+TGG 0.562878 8.3:+68152336 MS.gene08516:CDS
GGGAAATGATGTTGTGAATG+GGG 0.563425 8.3:-68151566 None:intergenic
ACCCAACAATAAATCACACT+TGG 0.575443 8.3:-68152278 None:intergenic
TGATGTCGTTGTTGTTGTCG+TGG 0.587065 8.3:-68151725 None:intergenic
TATGATGAGGCGTAATCGGA+TGG 0.604145 8.3:-68152051 None:intergenic
CGTGCCGGTTCCAGTTTCGG+TGG 0.607622 8.3:+68152135 MS.gene08516:CDS
ACAACGACATCAATATCCCA+TGG 0.615164 8.3:+68151736 MS.gene08516:CDS
GCACTTGTGACTGTGATGTG+AGG 0.630907 8.3:-68151893 None:intergenic
TGATGACGTTGTTGTTGACG+TGG 0.649666 8.3:-68151641 None:intergenic
TGATGACGTTGTTGTTGACG+TGG 0.649666 8.3:-68151683 None:intergenic
GGTATGCAAGCCATAAGATG+AGG 0.669740 8.3:-68151859 None:intergenic
ATGGTTTCTATATGACACAA+AGG 0.671270 8.3:-68152032 None:intergenic
AGCGATGGAAGCATATGATG+AGG 0.674282 8.3:-68152064 None:intergenic
GGATTATAATGCCAAGTACG+AGG 0.674453 8.3:-68151838 None:intergenic
CAATATCCCATGGAACACAA+AGG 0.680881 8.3:+68151746 MS.gene08516:CDS
GTTGGATGAGAATTATGGGG+TGG 0.688698 8.3:+68152177 MS.gene08516:CDS
GGAGAAGCATGAAACCGTGT+CGG 0.692283 8.3:+68152093 MS.gene08516:CDS
TAAAACTGGTGATACCGACA+CGG 0.699605 8.3:-68152107 None:intergenic
ATTGAGATTGACATGTCTGG+TGG 0.701504 8.3:-68151944 None:intergenic
GTTTCTATATGACACAAAGG+CGG 0.718743 8.3:-68152029 None:intergenic
GCATTAGGTACTCTATAACG+AGG 0.741839 8.3:-68151776 None:intergenic
CTGGTGGTGAAGTTGCATTG+AGG 0.741850 8.3:-68151928 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGCAATTTACAATCTTCATA+AGG + chr8.3:68151966-68151985 MS.gene08516:CDS 25.0%
ATGGTTTCTATATGACACAA+AGG - chr8.3:68152035-68152054 None:intergenic 30.0%
GCATTATAATCCTCATCTTA+TGG + chr8.3:68151849-68151868 MS.gene08516:CDS 30.0%
GGTGTGAAACTTATATTTCA+AGG + chr8.3:68152202-68152221 MS.gene08516:CDS 30.0%
TAAATGGATACACGTTTGTT+TGG - chr8.3:68151995-68152014 None:intergenic 30.0%
TGAAACTTATATTTCAAGGC+AGG + chr8.3:68152206-68152225 MS.gene08516:CDS 30.0%
TTATTGTTGGGTTTGAAGAA+AGG + chr8.3:68152289-68152308 MS.gene08516:CDS 30.0%
ACCCAACAATAAATCACACT+TGG - chr8.3:68152281-68152300 None:intergenic 35.0%
AGGGAAATGATGTTGTGAAT+GGG - chr8.3:68151570-68151589 None:intergenic 35.0%
GCCAAGTGTGATTTATTGTT+GGG + chr8.3:68152277-68152296 MS.gene08516:CDS 35.0%
GTGTATCCATTTACTTTGAC+AGG + chr8.3:68152002-68152021 MS.gene08516:CDS 35.0%
GTTTCTATATGACACAAAGG+CGG - chr8.3:68152032-68152051 None:intergenic 35.0%
TAGGGAAATGATGTTGTGAA+TGG - chr8.3:68151571-68151590 None:intergenic 35.0%
TATGTATCCACATCACAAAC+TGG - chr8.3:68152346-68152365 None:intergenic 35.0%
TGCCAAGTGTGATTTATTGT+TGG + chr8.3:68152276-68152295 MS.gene08516:CDS 35.0%
TTATATTTCAAGGCAGGTTG+AGG + chr8.3:68152212-68152231 MS.gene08516:CDS 35.0%
! GTTGTTGTGGCATTTTGTTA+GGG - chr8.3:68151589-68151608 None:intergenic 35.0%
! TGGTGTTGGATGAGAATTAT+GGG + chr8.3:68152173-68152192 MS.gene08516:CDS 35.0%
! TGTTGTTGTGGCATTTTGTT+AGG - chr8.3:68151590-68151609 None:intergenic 35.0%
!! TTGGTGTTGGATGAGAATTA+TGG + chr8.3:68152172-68152191 MS.gene08516:CDS 35.0%
!!! TGTTTTGAATGGTTTGGTGT+TGG + chr8.3:68152159-68152178 MS.gene08516:CDS 35.0%
AACCATACCTTTGTGTTCCA+TGG - chr8.3:68151756-68151775 None:intergenic 40.0%
ACAACGACATCAATATCCCA+TGG + chr8.3:68151736-68151755 MS.gene08516:CDS 40.0%
ACAATCTTCTTCCTCGTACT+TGG + chr8.3:68151827-68151846 MS.gene08516:CDS 40.0%
ACCATACCTTTGTGTTCCAT+GGG - chr8.3:68151755-68151774 None:intergenic 40.0%
ACGTCATCAATATCCCGAAA+TGG + chr8.3:68151655-68151674 MS.gene08516:CDS 40.0%
AGCATATGATGAGGCGTAAT+CGG - chr8.3:68152058-68152077 None:intergenic 40.0%
ATTGAGATTGACATGTCTGG+TGG - chr8.3:68151947-68151966 None:intergenic 40.0%
CAATATCCCATGGAACACAA+AGG + chr8.3:68151746-68151765 MS.gene08516:CDS 40.0%
GCATTAGGTACTCTATAACG+AGG - chr8.3:68151779-68151798 None:intergenic 40.0%
GGAAAGCCTGTCAAAGTAAA+TGG - chr8.3:68152011-68152030 None:intergenic 40.0%
GGATTATAATGCCAAGTACG+AGG - chr8.3:68151841-68151860 None:intergenic 40.0%
GGGAAATGATGTTGTGAATG+GGG - chr8.3:68151569-68151588 None:intergenic 40.0%
TAAAACTGGTGATACCGACA+CGG - chr8.3:68152110-68152129 None:intergenic 40.0%
TATGCTTCCATCGCTTTACT+TGG + chr8.3:68152072-68152091 MS.gene08516:CDS 40.0%
TGCATTGAGATTGACATGTC+TGG - chr8.3:68151950-68151969 None:intergenic 40.0%
TTCAAAACATCCACCGAAAC+TGG - chr8.3:68152148-68152167 None:intergenic 40.0%
TTGACGTGGTTGTTCCATTT+CGG - chr8.3:68151630-68151649 None:intergenic 40.0%
TTGTCGTGGTTGTTCCATTT+CGG - chr8.3:68151714-68151733 None:intergenic 40.0%
! CATATGCAGTGTTCACGTTT+TGG - chr8.3:68151803-68151822 None:intergenic 40.0%
! GGTGTTGGATGAGAATTATG+GGG + chr8.3:68152174-68152193 MS.gene08516:CDS 40.0%
! GGTTTGAAGAAAGGTCTTGT+GGG + chr8.3:68152298-68152317 MS.gene08516:CDS 40.0%
!! ACTGTGATGTGAGGTTTTGA+GGG - chr8.3:68151887-68151906 None:intergenic 40.0%
!! GGTGGATGTTTTGAATGGTT+TGG + chr8.3:68152153-68152172 MS.gene08516:CDS 40.0%
!! GTTTCGGTGGATGTTTTGAA+TGG + chr8.3:68152148-68152167 MS.gene08516:CDS 40.0%
!!! TGTTCACGTTTTGGAGCATT+AGG - chr8.3:68151794-68151813 None:intergenic 40.0%
ACGTCGTCAATACCCTGAAA+TGG + chr8.3:68151613-68151632 MS.gene08516:CDS 45.0%
GACGTGGTTGTTCCATTTCA+GGG - chr8.3:68151628-68151647 None:intergenic 45.0%
GATGAGAATTATGGGGTGGT+GGG + chr8.3:68152181-68152200 MS.gene08516:CDS 45.0%
GATGTTGTGAATGGGGTTGT+TGG - chr8.3:68151562-68151581 None:intergenic 45.0%
GGGTTTGAAGAAAGGTCTTG+TGG + chr8.3:68152297-68152316 MS.gene08516:CDS 45.0%
GGTATGCAAGCCATAAGATG+AGG - chr8.3:68151862-68151881 None:intergenic 45.0%
GTGGGTCAGATTCCTCTTAT+TGG + chr8.3:68152316-68152335 MS.gene08516:CDS 45.0%
GTTGGATGAGAATTATGGGG+TGG + chr8.3:68152177-68152196 MS.gene08516:CDS 45.0%
TATGATGAGGCGTAATCGGA+TGG - chr8.3:68152054-68152073 None:intergenic 45.0%
TCCCATGGAACACAAAGGTA+TGG + chr8.3:68151751-68151770 MS.gene08516:CDS 45.0%
TGACGTGGTTGTTCCATTTC+AGG - chr8.3:68151629-68151648 None:intergenic 45.0%
TGACGTGGTTGTTCCATTTC+GGG - chr8.3:68151629-68151648 None:intergenic 45.0%
TGCTTCTCCAAGTAAAGCGA+TGG - chr8.3:68152082-68152101 None:intergenic 45.0%
TGTCGTGGTTGTTCCATTTC+GGG - chr8.3:68151713-68151732 None:intergenic 45.0%
! AGCGATGGAAGCATATGATG+AGG - chr8.3:68152067-68152086 None:intergenic 45.0%
! CAAACTGGAGCACCAATAAG+AGG - chr8.3:68152331-68152350 None:intergenic 45.0%
! GACTGTGATGTGAGGTTTTG+AGG - chr8.3:68151888-68151907 None:intergenic 45.0%
! GTCACAAGTGCTGCAATCTA+TGG + chr8.3:68151905-68151924 MS.gene08516:CDS 45.0%
! TATTTCAAGGCAGGTTGAGG+TGG + chr8.3:68152215-68152234 MS.gene08516:CDS 45.0%
! TGACGACGTTGTTGTTGTTG+TGG - chr8.3:68151602-68151621 None:intergenic 45.0%
! TGATGACGTTGTTGTTGACG+TGG - chr8.3:68151644-68151663 None:intergenic 45.0%
! TGATGTCGTTGTTGTTGTCG+TGG - chr8.3:68151728-68151747 None:intergenic 45.0%
! TGTGAATGGGGTTGTTGGTT+AGG - chr8.3:68151557-68151576 None:intergenic 45.0%
!! GTGTCGGTATCACCAGTTTT+AGG + chr8.3:68152109-68152128 MS.gene08516:CDS 45.0%
!!! TCGGTATCACCAGTTTTAGG+AGG + chr8.3:68152112-68152131 MS.gene08516:CDS 45.0%
!!! TGATGTGAGGTTTTGAGGGA+TGG - chr8.3:68151883-68151902 None:intergenic 45.0%
ACATCCACCGAAACTGGAAC+CGG - chr8.3:68152142-68152161 None:intergenic 50.0%
GCACTTGTGACTGTGATGTG+AGG - chr8.3:68151896-68151915 None:intergenic 50.0%
GGATGAGAATTATGGGGTGG+TGG + chr8.3:68152180-68152199 MS.gene08516:CDS 50.0%
TGGTGCTCCAGTTTGTGATG+TGG + chr8.3:68152336-68152355 MS.gene08516:CDS 50.0%
! CTGGTGGTGAAGTTGCATTG+AGG - chr8.3:68151931-68151950 None:intergenic 50.0%
! GGAGAAGCATGAAACCGTGT+CGG + chr8.3:68152093-68152112 MS.gene08516:CDS 50.0%
! GGCAGGTTGAGGTGGAAAAC+TGG + chr8.3:68152223-68152242 MS.gene08516:CDS 55.0%
!!! ACCAGTTTTAGGAGGCGTGC+CGG + chr8.3:68152120-68152139 MS.gene08516:CDS 55.0%
ACCGGCACGCCTCCTAAAAC+TGG - chr8.3:68152124-68152143 None:intergenic 60.0%
!! AGGCGTGCCGGTTCCAGTTT+CGG + chr8.3:68152132-68152151 MS.gene08516:CDS 60.0%
!! CGTGCCGGTTCCAGTTTCGG+TGG + chr8.3:68152135-68152154 MS.gene08516:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 68151515 68152366 68151515 ID=MS.gene08516
chr8.3 mRNA 68151515 68152366 68151515 ID=MS.gene08516.t1;Parent=MS.gene08516
chr8.3 exon 68151515 68152366 68151515 ID=MS.gene08516.t1.exon1;Parent=MS.gene08516.t1
chr8.3 CDS 68151515 68152366 68151515 ID=cds.MS.gene08516.t1;Parent=MS.gene08516.t1
Gene Sequence

>MS.gene08516

ATGGAAGACGAAATAATGCATATAGAACGTCAAGAAGATCCTAACCAACAACCCCATTCACAACATCATTTCCCTAACAAAATGCCACAACAACAACAACGTCGTCAATACCCTGAAATGGAACAACCACGTCAACAACAACGTCATCAATATCCCGAAATGGAACAACCACGTCAACAACAACGTCATCAATATCCCGAAATGGAACAACCACGACAACAACAACGACATCAATATCCCATGGAACACAAAGGTATGGTTCCTCGTTATAGAGTACCTAATGCTCCAAAACGTGAACACTGCATATGCATCACAATCTTCTTCCTCGTACTTGGCATTATAATCCTCATCTTATGGCTTGCATACCATCCCTCAAAACCTCACATCACAGTCACAAGTGCTGCAATCTATGGCCTCAATGCAACTTCACCACCAGACATGTCAATCTCAATGCAATTTACAATCTTCATAAGGAATCCAAACAAACGTGTATCCATTTACTTTGACAGGCTTTCCGCCTTTGTGTCATATAGAAACCATCCGATTACGCCTCATCATATGCTTCCATCGCTTTACTTGGAGAAGCATGAAACCGTGTCGGTATCACCAGTTTTAGGAGGCGTGCCGGTTCCAGTTTCGGTGGATGTTTTGAATGGTTTGGTGTTGGATGAGAATTATGGGGTGGTGGGTGTGAAACTTATATTTCAAGGCAGGTTGAGGTGGAAAACTGGTGAGATAAAATCTGCACATTATAATATGTATGCCAAGTGTGATTTATTGTTGGGTTTGAAGAAAGGTCTTGTGGGTCAGATTCCTCTTATTGGTGCTCCAGTTTGTGATGTGGATACATAA

Protein sequence

>MS.gene08516.t1

MEDEIMHIERQEDPNQQPHSQHHFPNKMPQQQQRRQYPEMEQPRQQQRHQYPEMEQPRQQQRHQYPEMEQPRQQQRHQYPMEHKGMVPRYRVPNAPKREHCICITIFFLVLGIIILILWLAYHPSKPHITVTSAAIYGLNATSPPDMSISMQFTIFIRNPNKRVSIYFDRLSAFVSYRNHPITPHHMLPSLYLEKHETVSVSPVLGGVPVPVSVDVLNGLVLDENYGVVGVKLIFQGRLRWKTGEIKSAHYNMYAKCDLLLGLKKGLVGQIPLIGAPVCDVDT