Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06060.t1 | XP_013447177.1 | 93.4 | 181 | 12 | 0 | 1 | 181 | 1 | 181 | 6.50E-87 | 330.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06060.t1 | A0A072TUK3 | 93.4 | 181 | 12 | 0 | 1 | 181 | 1 | 181 | 4.7e-87 | 330.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene06060 | MS.gene42636 | 0.800714 | 1.27E-48 | -1.69E-46 |
| MS.gene06060 | MS.gene71259 | 0.817298 | 3.55E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06060.t1 | MTR_8g099345 | 93.370 | 181 | 12 | 0 | 1 | 181 | 1 | 181 | 1.91e-122 | 343 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06060.t1 | AT1G64355 | 75.556 | 135 | 26 | 3 | 32 | 162 | 43 | 174 | 5.91e-66 | 201 |
| MS.gene06060.t1 | AT1G64355 | 66.990 | 103 | 25 | 4 | 32 | 128 | 43 | 142 | 7.14e-37 | 125 |
| MS.gene06060.t1 | AT1G64355 | 63.158 | 95 | 28 | 3 | 32 | 122 | 43 | 134 | 1.56e-31 | 111 |
Find 43 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAGTTCCAGCTGGAATTTA+TGG | 0.258898 | 4.1:-4731604 | MS.gene06060:intron |
| GGTGAGGTTAGGAGAAGTTT+TGG | 0.272109 | 4.1:+4731766 | None:intergenic |
| TTGAAGGGTTGATAAGTTTA+GGG | 0.291880 | 4.1:+4731996 | None:intergenic |
| GTTGAAGGGTTGATAAGTTT+AGG | 0.306773 | 4.1:+4731995 | None:intergenic |
| CACTAACTTGTTCATTGTGT+TGG | 0.314311 | 4.1:-4731258 | MS.gene06060:CDS |
| TTAAATGAAAATCACGTTGC+AGG | 0.357937 | 4.1:+4731966 | None:intergenic |
| ACTAACTTGTTCATTGTGTT+GGG | 0.374119 | 4.1:-4731257 | MS.gene06060:CDS |
| TTGACAGCTAAGGTGCATTA+TGG | 0.386555 | 4.1:-4731338 | MS.gene06060:intron |
| TGCCCCTGGGTGTTGATCCA+TGG | 0.395407 | 4.1:-4731880 | MS.gene06060:CDS |
| AATCAGAAAAGCTGGAAATT+CGG | 0.416724 | 4.1:-4731222 | MS.gene06060:CDS |
| TCTCCTAACCTCACCCTCTT+CGG | 0.418605 | 4.1:-4731758 | MS.gene06060:CDS |
| AAACCGAAGAGGGTGAGGTT+AGG | 0.440423 | 4.1:+4731755 | None:intergenic |
| CGCGATGCTCTGTGAATCAA+TGG | 0.440579 | 4.1:+4731850 | None:intergenic |
| GATCGTGTGTGGATTGCCCC+TGG | 0.440978 | 4.1:-4731894 | MS.gene06060:CDS |
| TCTAATGTGGATTGGTTACA+TGG | 0.443829 | 4.1:-4731305 | MS.gene06060:CDS |
| CTTCTTTGTCTAATGTGGAT+TGG | 0.447286 | 4.1:-4731313 | MS.gene06060:CDS |
| TGGATTTGGTAATGAAGTAC+AGG | 0.449152 | 4.1:+4731786 | None:intergenic |
| ATGTGGATTGGTTACATGGT+GGG | 0.450371 | 4.1:-4731301 | MS.gene06060:CDS |
| TGGAACTTCTTTGTCTAATG+TGG | 0.464776 | 4.1:-4731318 | MS.gene06060:CDS |
| AGAGAGGGAATCAGAAAAGC+TGG | 0.466045 | 4.1:-4731230 | MS.gene06060:CDS |
| GCCCCTGGGTGTTGATCCAT+GGG | 0.473465 | 4.1:-4731879 | MS.gene06060:CDS |
| CGTTGCAGGAAGGTCGTTGA+AGG | 0.474175 | 4.1:+4731980 | None:intergenic |
| ATTGTGTTGGGTTTGAGAGA+GGG | 0.496509 | 4.1:-4731245 | MS.gene06060:CDS |
| GACTCACCATAAATTCCAGC+TGG | 0.525068 | 4.1:+4731598 | None:intergenic |
| AAGTACAGGAAACCAATGTA+AGG | 0.527193 | 4.1:+4731800 | None:intergenic |
| CATTGTGTTGGGTTTGAGAG+AGG | 0.529587 | 4.1:-4731246 | MS.gene06060:CDS |
| GTGCCCATGGATCAACACCC+AGG | 0.532974 | 4.1:+4731876 | None:intergenic |
| CAAGTTAGTGAGAGTGAGAA+GGG | 0.535305 | 4.1:+4731271 | None:intergenic |
| TGTGGATTGGTTACATGGTG+GGG | 0.539691 | 4.1:-4731300 | MS.gene06060:CDS |
| AGTAGAAACCGAAGAGGGTG+AGG | 0.547358 | 4.1:+4731750 | None:intergenic |
| TGCCCATGGATCAACACCCA+GGG | 0.560771 | 4.1:+4731877 | None:intergenic |
| AGCAACTAGACCTTCATCCC+AGG | 0.562545 | 4.1:+4731167 | None:intergenic |
| ATCGTGTGTGGATTGCCCCT+GGG | 0.578639 | 4.1:-4731893 | MS.gene06060:CDS |
| AATGTGGATTGGTTACATGG+TGG | 0.588190 | 4.1:-4731302 | MS.gene06060:CDS |
| ATGAAAATCACGTTGCAGGA+AGG | 0.588711 | 4.1:+4731970 | None:intergenic |
| GATTTACAGAGAGATACAAC+AGG | 0.591539 | 4.1:+4732019 | None:intergenic |
| ACAAGTTAGTGAGAGTGAGA+AGG | 0.592216 | 4.1:+4731270 | None:intergenic |
| TGAAGGGTTGATAAGTTTAG+GGG | 0.614651 | 4.1:+4731997 | None:intergenic |
| GTTGCAGGAAGGTCGTTGAA+GGG | 0.617837 | 4.1:+4731981 | None:intergenic |
| AAGAGAACAGTGATCGTGTG+TGG | 0.618841 | 4.1:-4731905 | MS.gene06060:CDS |
| GAATCAATGGAAGGTGCCCA+TGG | 0.621299 | 4.1:+4731863 | None:intergenic |
| ATGCTCTGTGAATCAATGGA+AGG | 0.666030 | 4.1:+4731854 | None:intergenic |
| GCCCATGGATCAACACCCAG+GGG | 0.698475 | 4.1:+4731878 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAATAAATAAATAAAA+AGG | + | chr4.1:4731591-4731610 | None:intergenic | 0.0% |
| !! | ATAAATAAATAAAAAGGAAA+AGG | + | chr4.1:4731585-4731604 | None:intergenic | 10.0% |
| !! | TAATTTAATCATTTCAAATG+TGG | + | chr4.1:4731840-4731859 | None:intergenic | 15.0% |
| !!! | CTCTAATTGAACTTTATTTT+CGG | + | chr4.1:4731657-4731676 | None:intergenic | 20.0% |
| ! | AAGAAAAGAAAACTAACTTG+TGG | + | chr4.1:4731515-4731534 | None:intergenic | 25.0% |
| ! | ATTACTTATGTTTAGTTGTG+TGG | - | chr4.1:4731856-4731875 | MS.gene06060:CDS | 25.0% |
| ! | TTGATTTATTATGATTCAGC+TGG | - | chr4.1:4731769-4731788 | MS.gene06060:CDS | 25.0% |
| ! | TTTAATTGTTCTGTTTGGTT+CGG | - | chr4.1:4731792-4731811 | MS.gene06060:CDS | 25.0% |
| !!! | TTTTCTTTGTTTCCTTACAT+TGG | - | chr4.1:4731406-4731425 | MS.gene06060:intron | 25.0% |
| AATCAGAAAAGCTGGAAATT+CGG | - | chr4.1:4731996-4732015 | MS.gene06060:CDS | 30.0% | |
| TTAAATGAAAATCACGTTGC+AGG | + | chr4.1:4731255-4731274 | None:intergenic | 30.0% | |
| !! | ACTAACTTGTTCATTGTGTT+GGG | - | chr4.1:4731961-4731980 | MS.gene06060:CDS | 30.0% |
| !! | TTGAAGGGTTGATAAGTTTA+GGG | + | chr4.1:4731225-4731244 | None:intergenic | 30.0% |
| !!! | CAATGTTTTAAAAACCGAAC+TGG | - | chr4.1:4731685-4731704 | MS.gene06060:intron | 30.0% |
| !!! | GGTTCGGTTTTTAGAATATT+GGG | - | chr4.1:4731808-4731827 | MS.gene06060:CDS | 30.0% |
| !!! | TGGTTCGGTTTTTAGAATAT+TGG | - | chr4.1:4731807-4731826 | MS.gene06060:CDS | 30.0% |
| AAGTACAGGAAACCAATGTA+AGG | + | chr4.1:4731421-4731440 | None:intergenic | 35.0% | |
| CAAAAAGTAGAAACCGAAGA+GGG | + | chr4.1:4731476-4731495 | None:intergenic | 35.0% | |
| CTTCTTTGTCTAATGTGGAT+TGG | - | chr4.1:4731905-4731924 | MS.gene06060:CDS | 35.0% | |
| GATTTACAGAGAGATACAAC+AGG | + | chr4.1:4731202-4731221 | None:intergenic | 35.0% | |
| TGGAAATTCGGAGAAAAACA+CGG | - | chr4.1:4732008-4732027 | MS.gene06060:CDS | 35.0% | |
| TGGAACTTCTTTGTCTAATG+TGG | - | chr4.1:4731900-4731919 | MS.gene06060:CDS | 35.0% | |
| ! | GCTGGTTTAATTGTTCTGTT+TGG | - | chr4.1:4731787-4731806 | MS.gene06060:CDS | 35.0% |
| !! | CACTAACTTGTTCATTGTGT+TGG | - | chr4.1:4731960-4731979 | MS.gene06060:CDS | 35.0% |
| !! | GTTGAAGGGTTGATAAGTTT+AGG | + | chr4.1:4731226-4731245 | None:intergenic | 35.0% |
| !! | TACTTTTTGCTCGCTTTTGT+TGG | - | chr4.1:4731487-4731506 | MS.gene06060:intron | 35.0% |
| !! | TCTAATGTGGATTGGTTACA+TGG | - | chr4.1:4731913-4731932 | MS.gene06060:CDS | 35.0% |
| !! | TGAAGGGTTGATAAGTTTAG+GGG | + | chr4.1:4731224-4731243 | None:intergenic | 35.0% |
| !! | TGGATTTGGTAATGAAGTAC+AGG | + | chr4.1:4731435-4731454 | None:intergenic | 35.0% |
| !!! | GTTAGGAGAAGTTTTGGATT+TGG | + | chr4.1:4731449-4731468 | None:intergenic | 35.0% |
| !!! | TTATTTTGAACAGTTCCAGC+TGG | - | chr4.1:4731605-4731624 | MS.gene06060:intron | 35.0% |
| AACCGAACTGGACATTAAAC+CGG | - | chr4.1:4731697-4731716 | MS.gene06060:intron | 40.0% | |
| ACAAGTTAGTGAGAGTGAGA+AGG | + | chr4.1:4731951-4731970 | None:intergenic | 40.0% | |
| ACAGTTCCAGCTGGAATTTA+TGG | - | chr4.1:4731614-4731633 | MS.gene06060:intron | 40.0% | |
| ATGAAAATCACGTTGCAGGA+AGG | + | chr4.1:4731251-4731270 | None:intergenic | 40.0% | |
| ATGCTCTGTGAATCAATGGA+AGG | + | chr4.1:4731367-4731386 | None:intergenic | 40.0% | |
| ATTGTGTTGGGTTTGAGAGA+GGG | - | chr4.1:4731973-4731992 | MS.gene06060:CDS | 40.0% | |
| CAAGTTAGTGAGAGTGAGAA+GGG | + | chr4.1:4731950-4731969 | None:intergenic | 40.0% | |
| GCAAAAAGTAGAAACCGAAG+AGG | + | chr4.1:4731477-4731496 | None:intergenic | 40.0% | |
| TTGACAGCTAAGGTGCATTA+TGG | - | chr4.1:4731880-4731899 | MS.gene06060:CDS | 40.0% | |
| !! | AATGTGGATTGGTTACATGG+TGG | - | chr4.1:4731916-4731935 | MS.gene06060:CDS | 40.0% |
| !! | ATGTGGATTGGTTACATGGT+GGG | - | chr4.1:4731917-4731936 | MS.gene06060:CDS | 40.0% |
| AACTGGACATTAAACCGGCA+TGG | - | chr4.1:4731702-4731721 | MS.gene06060:intron | 45.0% | |
| AAGAGAACAGTGATCGTGTG+TGG | - | chr4.1:4731313-4731332 | MS.gene06060:CDS | 45.0% | |
| AGAGAGGGAATCAGAAAAGC+TGG | - | chr4.1:4731988-4732007 | MS.gene06060:CDS | 45.0% | |
| CATTGTGTTGGGTTTGAGAG+AGG | - | chr4.1:4731972-4731991 | MS.gene06060:CDS | 45.0% | |
| CGAAGGAGGAGAAAAAACCT+GGG | - | chr4.1:4732034-4732053 | MS.gene06060:CDS | 45.0% | |
| GAAAAACACGGAATCGAAGG+AGG | - | chr4.1:4732020-4732039 | MS.gene06060:CDS | 45.0% | |
| GACTCACCATAAATTCCAGC+TGG | + | chr4.1:4731623-4731642 | None:intergenic | 45.0% | |
| GGAGAAAAAACCTGGGATGA+AGG | - | chr4.1:4732041-4732060 | MS.gene06060:CDS | 45.0% | |
| GGAGAAAAACACGGAATCGA+AGG | - | chr4.1:4732017-4732036 | MS.gene06060:CDS | 45.0% | |
| TCGAAGGAGGAGAAAAAACC+TGG | - | chr4.1:4732033-4732052 | MS.gene06060:CDS | 45.0% | |
| TGTATGCTGTTGAACCATGC+CGG | + | chr4.1:4731719-4731738 | None:intergenic | 45.0% | |
| ! | GGTGAGGTTAGGAGAAGTTT+TGG | + | chr4.1:4731455-4731474 | None:intergenic | 45.0% |
| !! | TGCCGGTTTAATGTCCAGTT+CGG | + | chr4.1:4731702-4731721 | None:intergenic | 45.0% |
| !! | TGTGGATTGGTTACATGGTG+GGG | - | chr4.1:4731918-4731937 | MS.gene06060:CDS | 45.0% |
| AAACCGAAGAGGGTGAGGTT+AGG | + | chr4.1:4731466-4731485 | None:intergenic | 50.0% | |
| AGTAGAAACCGAAGAGGGTG+AGG | + | chr4.1:4731471-4731490 | None:intergenic | 50.0% | |
| CGCGATGCTCTGTGAATCAA+TGG | + | chr4.1:4731371-4731390 | None:intergenic | 50.0% | |
| GTTGCAGGAAGGTCGTTGAA+GGG | + | chr4.1:4731240-4731259 | None:intergenic | 50.0% | |
| GTTGTGTGGCTTGACAGCTA+AGG | - | chr4.1:4731870-4731889 | MS.gene06060:CDS | 50.0% | |
| TCTCCTAACCTCACCCTCTT+CGG | - | chr4.1:4731460-4731479 | MS.gene06060:intron | 50.0% | |
| !! | GAATCAATGGAAGGTGCCCA+TGG | + | chr4.1:4731358-4731377 | None:intergenic | 50.0% |
| CGTTGCAGGAAGGTCGTTGA+AGG | + | chr4.1:4731241-4731260 | None:intergenic | 55.0% | |
| TGCCCATGGATCAACACCCA+GGG | + | chr4.1:4731344-4731363 | None:intergenic | 55.0% | |
| ! | ATCGTGTGTGGATTGCCCCT+GGG | - | chr4.1:4731325-4731344 | MS.gene06060:CDS | 55.0% |
| GCCCATGGATCAACACCCAG+GGG | + | chr4.1:4731343-4731362 | None:intergenic | 60.0% | |
| GTGCCCATGGATCAACACCC+AGG | + | chr4.1:4731345-4731364 | None:intergenic | 60.0% | |
| ! | GATCGTGTGTGGATTGCCCC+TGG | - | chr4.1:4731324-4731343 | MS.gene06060:CDS | 60.0% |
| !! | GCCCCTGGGTGTTGATCCAT+GGG | - | chr4.1:4731339-4731358 | MS.gene06060:intron | 60.0% |
| !! | TGCCCCTGGGTGTTGATCCA+TGG | - | chr4.1:4731338-4731357 | MS.gene06060:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 4731172 | 4732068 | 4731172 | ID=MS.gene06060 |
| chr4.1 | mRNA | 4731172 | 4732068 | 4731172 | ID=MS.gene06060.t1;Parent=MS.gene06060 |
| chr4.1 | exon | 4731723 | 4732068 | 4731723 | ID=MS.gene06060.t1.exon1;Parent=MS.gene06060.t1 |
| chr4.1 | CDS | 4731723 | 4732068 | 4731723 | ID=cds.MS.gene06060.t1;Parent=MS.gene06060.t1 |
| chr4.1 | exon | 4731605 | 4731622 | 4731605 | ID=MS.gene06060.t1.exon2;Parent=MS.gene06060.t1 |
| chr4.1 | CDS | 4731605 | 4731622 | 4731605 | ID=cds.MS.gene06060.t1;Parent=MS.gene06060.t1 |
| chr4.1 | exon | 4731172 | 4731353 | 4731172 | ID=MS.gene06060.t1.exon3;Parent=MS.gene06060.t1 |
| chr4.1 | CDS | 4731172 | 4731353 | 4731172 | ID=cds.MS.gene06060.t1;Parent=MS.gene06060.t1 |
| Gene Sequence |
| Protein sequence |
