Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06065.t1 | XP_013447184.1 | 94.7 | 95 | 5 | 0 | 1 | 95 | 1 | 95 | 2.80E-41 | 177.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06065.t1 | O64481 | 46.6 | 58 | 30 | 1 | 37 | 93 | 65 | 122 | 3.9e-07 | 55.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06065.t1 | A0A072TVK3 | 94.7 | 95 | 5 | 0 | 1 | 95 | 1 | 95 | 2.0e-41 | 177.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051789 | MS.gene06065 | 0.812326 | 4.48E-51 | -1.69E-46 |
| MS.gene06065 | MS.gene069581 | 0.818317 | 2.09E-52 | -1.69E-46 |
| MS.gene06065 | MS.gene32978 | 0.801348 | 9.39E-49 | -1.69E-46 |
| MS.gene06065 | MS.gene34106 | 0.803864 | 2.84E-49 | -1.69E-46 |
| MS.gene06065 | MS.gene62720 | 0.801099 | 1.06E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06065.t1 | MTR_8g099385 | 94.737 | 95 | 5 | 0 | 1 | 95 | 1 | 95 | 9.78e-62 | 182 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06065.t1 | AT2G19170 | 46.552 | 58 | 30 | 1 | 37 | 93 | 65 | 122 | 8.48e-11 | 57.0 |
| MS.gene06065.t1 | AT2G19170 | 46.552 | 58 | 30 | 1 | 37 | 93 | 65 | 122 | 8.48e-11 | 57.0 |
Find 9 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAAAGAAAAGTTCACTTT+TGG | 0.321241 | 4.1:-4703337 | None:intergenic |
| GATATCTTCTTGTAGCTTCC+TGG | 0.375321 | 4.1:-4703596 | None:intergenic |
| GCTCATCATGCTTTCTACTA+AGG | 0.476413 | 4.1:-4703553 | None:intergenic |
| TTAGCAAGCACTCTAGAGCC+AGG | 0.504266 | 4.1:+4703578 | MS.gene06065:CDS |
| ACGAGCGACTTCGAACTAAT+CGG | 0.522782 | 4.1:+4703382 | MS.gene06065:CDS |
| ATTACACATTACTTGATCCT+CGG | 0.550610 | 4.1:-4703657 | None:intergenic |
| AATGTACTCAGATCTGCAAA+AGG | 0.568112 | 4.1:+4703800 | MS.gene06065:CDS |
| TGCAAAAGGAGTGAGAATTG+TGG | 0.605047 | 4.1:+4703814 | MS.gene06065:CDS |
| TTTAATTAGAACTAAGAGTG+GGG | 0.607456 | 4.1:+4703511 | MS.gene06065:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAACAAGATAATTAAGAAA+AGG | - | chr4.1:4703765-4703784 | None:intergenic | 15.0% |
| !! | TGTTACAAATAAAAAAACAA+AGG | - | chr4.1:4703426-4703445 | None:intergenic | 15.0% |
| !! | ATAAAATTGTACTTTATACG+AGG | - | chr4.1:4703687-4703706 | None:intergenic | 20.0% |
| !! | TGATATTTACACATATTATG+TGG | + | chr4.1:4703726-4703745 | MS.gene06065:intron | 20.0% |
| !!! | TTTTAATTAGAACTAAGAGT+GGG | + | chr4.1:4703510-4703529 | MS.gene06065:intron | 20.0% |
| ! | TAAGAAAAGGAAAACATCAT+AGG | - | chr4.1:4703752-4703771 | None:intergenic | 25.0% |
| ! | TTTAATTAGAACTAAGAGTG+GGG | + | chr4.1:4703511-4703530 | MS.gene06065:intron | 25.0% |
| !!! | CTTTTAATTAGAACTAAGAG+TGG | + | chr4.1:4703509-4703528 | MS.gene06065:intron | 25.0% |
| ATTACACATTACTTGATCCT+CGG | - | chr4.1:4703660-4703679 | None:intergenic | 30.0% | |
| ! | ATCTTGTTATGCATTCAAAC+AGG | + | chr4.1:4703775-4703794 | MS.gene06065:intron | 30.0% |
| ! | GACAAAGAAAAGTTCACTTT+TGG | - | chr4.1:4703340-4703359 | None:intergenic | 30.0% |
| !!! | TATTGTTGATGGTTTTACAG+TGG | + | chr4.1:4703628-4703647 | MS.gene06065:CDS | 30.0% |
| !!! | TCTTCTTTTGCTATTGTTGA+TGG | + | chr4.1:4703617-4703636 | MS.gene06065:CDS | 30.0% |
| AATGTACTCAGATCTGCAAA+AGG | + | chr4.1:4703800-4703819 | MS.gene06065:CDS | 35.0% | |
| ! | TTTTGCAGATCTGAGTACAT+TGG | - | chr4.1:4703801-4703820 | None:intergenic | 35.0% |
| GATATCTTCTTGTAGCTTCC+TGG | - | chr4.1:4703599-4703618 | None:intergenic | 40.0% | |
| GCTCATCATGCTTTCTACTA+AGG | - | chr4.1:4703556-4703575 | None:intergenic | 40.0% | |
| TGCAAAAGGAGTGAGAATTG+TGG | + | chr4.1:4703814-4703833 | MS.gene06065:CDS | 40.0% | |
| ! | TTTTACAGTGGAAATCACCG+AGG | + | chr4.1:4703640-4703659 | MS.gene06065:CDS | 40.0% |
| ACGAGCGACTTCGAACTAAT+CGG | + | chr4.1:4703382-4703401 | MS.gene06065:CDS | 45.0% | |
| !! | TTAGCAAGCACTCTAGAGCC+AGG | + | chr4.1:4703578-4703597 | MS.gene06065:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 4703327 | 4703859 | 4703327 | ID=MS.gene06065 |
| chr4.1 | mRNA | 4703327 | 4703859 | 4703327 | ID=MS.gene06065.t1;Parent=MS.gene06065 |
| chr4.1 | exon | 4703327 | 4703403 | 4703327 | ID=MS.gene06065.t1.exon1;Parent=MS.gene06065.t1 |
| chr4.1 | CDS | 4703327 | 4703403 | 4703327 | ID=cds.MS.gene06065.t1;Parent=MS.gene06065.t1 |
| chr4.1 | exon | 4703520 | 4703667 | 4703520 | ID=MS.gene06065.t1.exon2;Parent=MS.gene06065.t1 |
| chr4.1 | CDS | 4703520 | 4703667 | 4703520 | ID=cds.MS.gene06065.t1;Parent=MS.gene06065.t1 |
| chr4.1 | exon | 4703797 | 4703859 | 4703797 | ID=MS.gene06065.t1.exon3;Parent=MS.gene06065.t1 |
| chr4.1 | CDS | 4703797 | 4703859 | 4703797 | ID=cds.MS.gene06065.t1;Parent=MS.gene06065.t1 |
| Gene Sequence |
| Protein sequence |
