Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060845.t1 | RHN58493.1 | 67 | 221 | 40 | 4 | 1 | 213 | 66 | 261 | 6.40E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060845.t1 | A0A396I179 | 67.0 | 221 | 40 | 4 | 1 | 213 | 66 | 261 | 4.6e-43 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060843 | MS.gene060845 | 0.877074 | 8.41E-69 | -1.69E-46 |
| MS.gene060844 | MS.gene060845 | 0.831594 | 1.53E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060845.t1 | MTR_4g009870 | 90.312 | 320 | 10 | 2 | 149 | 448 | 1 | 319 | 0.0 | 552 |
| MS.gene060845.t1 | MTR_4g009870 | 33.811 | 349 | 185 | 9 | 60 | 402 | 59 | 367 | 3.08e-26 | 109 |
| MS.gene060845.t1 | MTR_4g009870 | 87.333 | 300 | 9 | 2 | 149 | 448 | 1 | 271 | 9.40e-177 | 498 |
| MS.gene060845.t1 | MTR_4g009870 | 29.863 | 365 | 200 | 6 | 60 | 423 | 10 | 319 | 7.34e-25 | 105 |
| MS.gene060845.t1 | MTR_4g116170 | 56.092 | 476 | 156 | 5 | 1 | 448 | 65 | 515 | 9.08e-152 | 443 |
| MS.gene060845.t1 | MTR_4g009873 | 68.142 | 113 | 26 | 3 | 1 | 103 | 66 | 178 | 2.25e-37 | 135 |
| MS.gene060845.t1 | MTR_8g099415 | 25.714 | 385 | 255 | 11 | 63 | 433 | 115 | 482 | 1.07e-12 | 70.5 |
| MS.gene060845.t1 | MTR_8g099415 | 25.714 | 385 | 255 | 11 | 63 | 433 | 115 | 482 | 1.27e-12 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060845.t1 | AT1G15740 | 60.377 | 477 | 135 | 6 | 1 | 448 | 73 | 524 | 7.88e-165 | 477 |
| MS.gene060845.t1 | AT4G23840 | 25.117 | 426 | 195 | 10 | 132 | 433 | 62 | 487 | 4.57e-16 | 80.9 |
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATGCTTGGATTCACTTTC+AGG | 0.068243 | 8.4:-23198936 | MS.gene060845:intron |
| GTCACAGTAATTAAGATTTA+AGG | 0.141721 | 8.4:+23201849 | None:intergenic |
| ATCTCTGAAGTCTCTTAATT+TGG | 0.152359 | 8.4:-23196234 | MS.gene060845:CDS |
| TGTAGTAAAGTCACTGATTT+TGG | 0.168024 | 8.4:-23199581 | MS.gene060845:CDS |
| GCGTCTGATGTCACTGATTT+TGG | 0.168253 | 8.4:-23201907 | MS.gene060845:CDS |
| GAAGCTTGCCCTCTTAAATT+TGG | 0.198047 | 8.4:-23198979 | MS.gene060845:CDS |
| CTCACTGACCTTGATTTGTT+TGG | 0.245633 | 8.4:-23195482 | MS.gene060845:CDS |
| TTAAGCAGGATTGACAAAAT+TGG | 0.248392 | 8.4:-23197618 | MS.gene060845:intron |
| CTTCTCTGGTCTTGTTAACT+TGG | 0.285104 | 8.4:-23200441 | MS.gene060845:CDS |
| CCAGTCCAGCATCAGTAATT+TGG | 0.287315 | 8.4:+23196204 | None:intergenic |
| TCCGATATGAAGCCTTTATC+AGG | 0.299738 | 8.4:-23200074 | MS.gene060845:intron |
| AATGGCCTAGAGCATTTATC+AGG | 0.312464 | 8.4:-23196866 | MS.gene060845:intron |
| TAACTTGGTCAAGTTGGATT+TGG | 0.325759 | 8.4:-23200426 | MS.gene060845:CDS |
| CCAGTCCATGATCTGAAATT+TGG | 0.332242 | 8.4:+23201827 | None:intergenic |
| GAGAGATGTCCTGGGATTCA+TGG | 0.355408 | 8.4:-23200404 | MS.gene060845:CDS |
| TTTGCTGATGCAGGTTTGAC+TGG | 0.365454 | 8.4:-23195506 | MS.gene060845:intron |
| TTGGATTTGGAGAGATGTCC+TGG | 0.400413 | 8.4:-23200413 | MS.gene060845:CDS |
| AACCTTGATTCATGTAAGAT+TGG | 0.403335 | 8.4:-23197587 | MS.gene060845:CDS |
| TGAGACTTTGACAATCTTGA+AGG | 0.404945 | 8.4:+23201875 | None:intergenic |
| AGATGCAACAAACCTGATAA+AGG | 0.406288 | 8.4:+23200062 | None:intergenic |
| CCAAATTACTGATGCTGGAC+TGG | 0.409005 | 8.4:-23196204 | MS.gene060845:CDS |
| TCTTGTAACATCTGCATGCT+TGG | 0.409080 | 8.4:-23198949 | MS.gene060845:CDS |
| TAAATCATATAGGCTCTGTT+TGG | 0.417581 | 8.4:-23202454 | MS.gene060845:CDS |
| TTTCTGACAGAGGGTGTGAT+AGG | 0.424800 | 8.4:-23198050 | MS.gene060845:CDS |
| TGGAGGCACTGTTCATTTCA+AGG | 0.427185 | 8.4:-23200384 | MS.gene060845:CDS |
| CAAGGAATGAGTGCCTTCTC+TGG | 0.429753 | 8.4:-23200455 | MS.gene060845:CDS |
| CATAACAAAGACAAAATTGC+AGG | 0.429924 | 8.4:+23201778 | None:intergenic |
| GACACCCTTCCAAATTTAAG+AGG | 0.430139 | 8.4:+23198970 | None:intergenic |
| GGCACTGTTCATTTCAAGGT+TGG | 0.436961 | 8.4:-23200380 | MS.gene060845:intron |
| TATATGTAATGTAATATTCC+AGG | 0.437860 | 8.4:-23200531 | MS.gene060845:CDS |
| TGTGACCAAATTTCAGATCA+TGG | 0.438486 | 8.4:-23201832 | MS.gene060845:CDS |
| ACCTGATAAAGGCTTCATAT+CGG | 0.443237 | 8.4:+23200073 | None:intergenic |
| AGATTCAAGTTTGACAGTGC+AGG | 0.445204 | 8.4:+23198087 | None:intergenic |
| GCTCTCCAGGTAAATCATAT+AGG | 0.456594 | 8.4:-23202464 | MS.gene060845:CDS |
| AAAGTTGAAAGTATTAAACT+TGG | 0.469983 | 8.4:-23197865 | MS.gene060845:CDS |
| AAAACTGTGGAGTTAATTGC+TGG | 0.470627 | 8.4:-23194759 | None:intergenic |
| ATTCAAGAATCTGAGGTCCT+TGG | 0.471185 | 8.4:-23194883 | MS.gene060845:CDS |
| GCTTACCAAATTACTGATGC+TGG | 0.472779 | 8.4:-23196209 | MS.gene060845:CDS |
| CAGAAAGATCTACGAGAGTA+AGG | 0.475496 | 8.4:+23201929 | None:intergenic |
| TCTTGTTAACTTGGTCAAGT+TGG | 0.476241 | 8.4:-23200432 | MS.gene060845:CDS |
| ATCACAGAACAGCAACCTTA+CGG | 0.480947 | 8.4:-23194784 | MS.gene060845:CDS |
| ATTTCGAGATTGTGCTCTCC+AGG | 0.486045 | 8.4:-23202477 | MS.gene060845:CDS |
| GAATTGTCTGATACTGAAGT+TGG | 0.487539 | 8.4:-23196893 | MS.gene060845:CDS |
| GCGCTAGGATCACAGATGTT+GGG | 0.491382 | 8.4:-23195460 | MS.gene060845:CDS |
| ACCTTGATTTGTTTGGCGCT+AGG | 0.494505 | 8.4:-23195475 | MS.gene060845:CDS |
| TCTAAAACTCAAACTTGTCA+AGG | 0.501200 | 8.4:+23200499 | None:intergenic |
| TCATAAGCAATTGATCTGCT+TGG | 0.506112 | 8.4:-23196915 | MS.gene060845:CDS |
| GGCGCTAGGATCACAGATGT+TGG | 0.508377 | 8.4:-23195461 | MS.gene060845:CDS |
| CACCAATCTTACATGAATCA+AGG | 0.510275 | 8.4:+23197585 | None:intergenic |
| TGATGAAGGATTGGTGAACT+TGG | 0.515944 | 8.4:-23197564 | MS.gene060845:CDS |
| GATTCAAGTTTGACAGTGCA+GGG | 0.517023 | 8.4:+23198088 | None:intergenic |
| CTTGCCCTCTTAAATTTGGA+AGG | 0.517960 | 8.4:-23198975 | MS.gene060845:CDS |
| CAGAGCCTATATGATTTACC+TGG | 0.523877 | 8.4:+23202459 | None:intergenic |
| CATGGGAACACATACTTGCA+AGG | 0.524260 | 8.4:+23196177 | None:intergenic |
| TCCTAGCGCCAAACAAATCA+AGG | 0.529517 | 8.4:+23195474 | None:intergenic |
| TCATGTAAGATTGGTGATGA+AGG | 0.532453 | 8.4:-23197578 | MS.gene060845:CDS |
| GAAGGATTGGTGAACTTGGC+AGG | 0.535990 | 8.4:-23197560 | MS.gene060845:intron |
| TCAAACCTGATAAATGCTCT+AGG | 0.548354 | 8.4:+23196861 | None:intergenic |
| AAGTTGAAAGTATTAAACTT+GGG | 0.557845 | 8.4:-23197864 | MS.gene060845:CDS |
| GCATGTTTAGCTCATTTGAA+AGG | 0.558223 | 8.4:-23197822 | MS.gene060845:intron |
| TTGTCAAGGTTTGACAGACC+TGG | 0.561427 | 8.4:+23200513 | None:intergenic |
| ATGGGAACACATACTTGCAA+GGG | 0.563020 | 8.4:+23196178 | None:intergenic |
| CAAGTTAACAAGACCAGAGA+AGG | 0.565092 | 8.4:+23200442 | None:intergenic |
| TAAGATTGGTGATGAAGGAT+TGG | 0.567788 | 8.4:-23197573 | MS.gene060845:CDS |
| AGCGGTGGATTGACTGATGC+TGG | 0.575698 | 8.4:-23194852 | MS.gene060845:CDS |
| TTGCCCTCTTAAATTTGGAA+GGG | 0.576199 | 8.4:-23198974 | MS.gene060845:CDS |
| AACTTGGGATTTAATGACAT+AGG | 0.577016 | 8.4:-23197849 | MS.gene060845:CDS |
| AATGATTCAATATCTGCTCA+AGG | 0.581564 | 8.4:-23200473 | MS.gene060845:CDS |
| CCAAATTTCAGATCATGGAC+TGG | 0.582601 | 8.4:-23201827 | MS.gene060845:CDS |
| AAACAGAATTCAAGAATCTG+AGG | 0.586780 | 8.4:-23194890 | MS.gene060845:intron |
| ACAGTGCCTCCATGAATCCC+AGG | 0.590376 | 8.4:+23200395 | None:intergenic |
| TGGATTTGGAGAGATGTCCT+GGG | 0.594319 | 8.4:-23200412 | MS.gene060845:CDS |
| GTGGTTTAAGGGAAATTGTG+TGG | 0.604223 | 8.4:-23196263 | MS.gene060845:intron |
| ACACCCTTCCAAATTTAAGA+GGG | 0.605875 | 8.4:+23198971 | None:intergenic |
| GATGCAATATTTCTGACAGA+GGG | 0.606799 | 8.4:-23198059 | MS.gene060845:CDS |
| ATTTGCTGACTTATATCCCG+TGG | 0.610485 | 8.4:+23202551 | None:intergenic |
| TGGAATCTCTCAATATGAAG+TGG | 0.616270 | 8.4:-23200115 | MS.gene060845:CDS |
| TCCACCGCTGCATATTTCCA+AGG | 0.622364 | 8.4:+23194866 | None:intergenic |
| CATGGACTGGAGTGTATCAG+TGG | 0.628538 | 8.4:-23201814 | MS.gene060845:CDS |
| GATACTGAAGTTGGAAACAA+TGG | 0.628892 | 8.4:-23196884 | MS.gene060845:CDS |
| TAAGTCATACACTCAAAATG+TGG | 0.632257 | 8.4:+23202426 | None:intergenic |
| TCCTTGGAAATATGCAGCGG+TGG | 0.643300 | 8.4:-23194867 | MS.gene060845:CDS |
| AGATGCAATATTTCTGACAG+AGG | 0.657280 | 8.4:-23198060 | MS.gene060845:CDS |
| CAACCTTACGGACAAAACTG+TGG | 0.677276 | 8.4:-23194772 | MS.gene060845:CDS |
| AGGTCCTTGGAAATATGCAG+CGG | 0.704864 | 8.4:-23194870 | MS.gene060845:CDS |
| AGATGTCCTGGGATTCATGG+AGG | 0.711361 | 8.4:-23200401 | MS.gene060845:CDS |
| CAAACAAATCAAGGTCAGTG+AGG | 0.728596 | 8.4:+23195483 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 23194760 | 23202606 | 23194760 | ID=MS.gene060845 |
| chr8.4 | mRNA | 23194760 | 23202606 | 23194760 | ID=MS.gene060845.t1;Parent=MS.gene060845 |
| chr8.4 | exon | 23202438 | 23202606 | 23202438 | ID=MS.gene060845.t1.exon1;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23202438 | 23202606 | 23202438 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23201790 | 23201958 | 23201790 | ID=MS.gene060845.t1.exon2;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23201790 | 23201958 | 23201790 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23200381 | 23200577 | 23200381 | ID=MS.gene060845.t1.exon3;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23200381 | 23200577 | 23200381 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23200075 | 23200149 | 23200075 | ID=MS.gene060845.t1.exon4;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23200075 | 23200149 | 23200075 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23199564 | 23199635 | 23199564 | ID=MS.gene060845.t1.exon5;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23199564 | 23199635 | 23199564 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23198937 | 23199008 | 23198937 | ID=MS.gene060845.t1.exon6;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23198937 | 23199008 | 23198937 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23198043 | 23198114 | 23198043 | ID=MS.gene060845.t1.exon7;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23198043 | 23198114 | 23198043 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23197823 | 23197894 | 23197823 | ID=MS.gene060845.t1.exon8;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23197823 | 23197894 | 23197823 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23197561 | 23197632 | 23197561 | ID=MS.gene060845.t1.exon9;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23197561 | 23197632 | 23197561 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23196867 | 23196938 | 23196867 | ID=MS.gene060845.t1.exon10;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23196867 | 23196938 | 23196867 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23196192 | 23196275 | 23196192 | ID=MS.gene060845.t1.exon11;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23196192 | 23196275 | 23196192 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23195444 | 23195515 | 23195444 | ID=MS.gene060845.t1.exon12;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23195444 | 23195515 | 23195444 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| chr8.4 | exon | 23194760 | 23194906 | 23194760 | ID=MS.gene060845.t1.exon13;Parent=MS.gene060845.t1 |
| chr8.4 | CDS | 23194760 | 23194906 | 23194760 | ID=cds.MS.gene060845.t1;Parent=MS.gene060845.t1 |
| Gene Sequence |
| Protein sequence |
