Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061462.t1 | XP_003620577.2 | 92.2 | 77 | 6 | 0 | 1 | 77 | 281 | 357 | 5.90E-31 | 143.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061462.t1 | Q9SL78 | 36.9 | 111 | 50 | 3 | 1 | 91 | 277 | 387 | 5.6e-06 | 52.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061462.t1 | G7KNZ9 | 92.2 | 77 | 6 | 0 | 1 | 77 | 281 | 357 | 4.3e-31 | 143.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057102 | MS.gene061462 | 0.812238 | 4.69E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene061462 | -0.817215 | 3.70E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061462.t1 | MTR_6g087150 | 92.208 | 77 | 6 | 0 | 1 | 77 | 281 | 357 | 2.10e-45 | 151 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAATGCTTCATCAAGTAAT+AGG | 0.316134 | 6.1:+77535864 | MS.gene061462:CDS |
| AGGAAAGAAGGAGGAAGCTT+TGG | 0.319725 | 6.1:+77536032 | MS.gene061462:CDS |
| TCAGCTGTTCTTCTTGCAAT+TGG | 0.337125 | 6.1:-77536058 | None:intergenic |
| AACTTTAATTTCTAGATATG+TGG | 0.431601 | 6.1:+77536343 | MS.gene061462:intron |
| GATTATCCTCGAGGTTCAGC+AGG | 0.433165 | 6.1:+77536185 | MS.gene061462:CDS |
| ACTCCACGCGGTGGGAGCCT+TGG | 0.440403 | 6.1:+77536394 | MS.gene061462:CDS |
| TCTTGAAAATAATCTTAAAG+AGG | 0.469161 | 6.1:+77536012 | MS.gene061462:CDS |
| ATAATCTTAAAGAGGAAAGA+AGG | 0.482860 | 6.1:+77536020 | MS.gene061462:CDS |
| CTCCCACCGCGTGGAGTCTG+AGG | 0.523289 | 6.1:-77536388 | None:intergenic |
| TTGCAAGAAGAACAGCTGAA+TGG | 0.523336 | 6.1:+77536062 | MS.gene061462:CDS |
| AGATCAGTGTCAGAAATCAC+TGG | 0.561549 | 6.1:+77535932 | MS.gene061462:CDS |
| AAGTAATAGGTTCAACAACA+TGG | 0.572303 | 6.1:+77535877 | MS.gene061462:CDS |
| AAACTGTGCTCGAGTGTCCA+AGG | 0.585029 | 6.1:-77536411 | None:intergenic |
| CTCCTCAGACTCCACGCGGT+GGG | 0.586815 | 6.1:+77536386 | MS.gene061462:CDS |
| CCTCCTCAGACTCCACGCGG+TGG | 0.601133 | 6.1:+77536385 | MS.gene061462:CDS |
| ATCTTAAAGAGGAAAGAAGG+AGG | 0.605571 | 6.1:+77536023 | MS.gene061462:CDS |
| CCACCGCGTGGAGTCTGAGG+AGG | 0.614425 | 6.1:-77536385 | None:intergenic |
| TGTCCAAGGCTCCCACCGCG+TGG | 0.644858 | 6.1:-77536397 | None:intergenic |
| ATTATCCTCGAGGTTCAGCA+GGG | 0.647182 | 6.1:+77536186 | MS.gene061462:CDS |
| TCTGACCCTGCTGAACCTCG+AGG | 0.684689 | 6.1:-77536191 | None:intergenic |
| AACTCATAGAATGCTTACAG+AGG | 0.693639 | 6.1:+77536225 | MS.gene061462:CDS |
| TATCCTCCTCAGACTCCACG+CGG | 0.752468 | 6.1:+77536382 | MS.gene061462:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATATCTAGAAATTAAAGT+TGG | - | chr6.1:77536344-77536363 | None:intergenic | 20.0% |
| !! | TCAAAAATTCAAGATTATGA+TGG | + | chr6.1:77535902-77535921 | MS.gene061462:CDS | 20.0% |
| !! | TCTTGAAAATAATCTTAAAG+AGG | + | chr6.1:77536012-77536031 | MS.gene061462:CDS | 20.0% |
| !!! | AACTTTAATTTCTAGATATG+TGG | + | chr6.1:77536343-77536362 | MS.gene061462:intron | 20.0% |
| ! | ATAATCTTAAAGAGGAAAGA+AGG | + | chr6.1:77536020-77536039 | MS.gene061462:CDS | 25.0% |
| ! | TTAATGCTTCATCAAGTAAT+AGG | + | chr6.1:77535864-77535883 | MS.gene061462:CDS | 25.0% |
| !!! | AGATTTTGACATGAAGAAAA+GGG | + | chr6.1:77535970-77535989 | MS.gene061462:CDS | 25.0% |
| !!! | TTCTTTAGGTACTTACTTTT+AGG | + | chr6.1:77536163-77536182 | MS.gene061462:intron | 25.0% |
| AAGTAATAGGTTCAACAACA+TGG | + | chr6.1:77535877-77535896 | MS.gene061462:CDS | 30.0% | |
| GGTTTGTCAATAGAGAAAAA+AGG | + | chr6.1:77536083-77536102 | MS.gene061462:intron | 30.0% | |
| GTACCTAAAGAAAGTGAAAA+TGG | - | chr6.1:77536155-77536174 | None:intergenic | 30.0% | |
| ! | AATACATGATTTGTACAACG+AGG | + | chr6.1:77536250-77536269 | MS.gene061462:intron | 30.0% |
| ! | AGTCCATTTTCACTTTCTTT+AGG | + | chr6.1:77536149-77536168 | MS.gene061462:intron | 30.0% |
| ! | GTAAGCATTCTATGAGTTTT+AGG | - | chr6.1:77536223-77536242 | None:intergenic | 30.0% |
| !! | GAGATTTTGACATGAAGAAA+AGG | + | chr6.1:77535969-77535988 | MS.gene061462:CDS | 30.0% |
| AACTCATAGAATGCTTACAG+AGG | + | chr6.1:77536225-77536244 | MS.gene061462:CDS | 35.0% | |
| ATCTTAAAGAGGAAAGAAGG+AGG | + | chr6.1:77536023-77536042 | MS.gene061462:CDS | 35.0% | |
| !!! | TACTTTTAGGATTATCCTCG+AGG | + | chr6.1:77536176-77536195 | MS.gene061462:CDS | 35.0% |
| AGATCAGTGTCAGAAATCAC+TGG | + | chr6.1:77535932-77535951 | MS.gene061462:CDS | 40.0% | |
| TCAGCTGTTCTTCTTGCAAT+TGG | - | chr6.1:77536061-77536080 | None:intergenic | 40.0% | |
| TGAGCTGAATTCTTATCGAC+TGG | + | chr6.1:77536317-77536336 | MS.gene061462:intron | 40.0% | |
| TTGCAAGAAGAACAGCTGAA+TGG | + | chr6.1:77536062-77536081 | MS.gene061462:CDS | 40.0% | |
| AGGAAAGAAGGAGGAAGCTT+TGG | + | chr6.1:77536032-77536051 | MS.gene061462:CDS | 45.0% | |
| ATTATCCTCGAGGTTCAGCA+GGG | + | chr6.1:77536186-77536205 | MS.gene061462:CDS | 45.0% | |
| AAACTGTGCTCGAGTGTCCA+AGG | - | chr6.1:77536414-77536433 | None:intergenic | 50.0% | |
| GATTATCCTCGAGGTTCAGC+AGG | + | chr6.1:77536185-77536204 | MS.gene061462:CDS | 50.0% | |
| TATCCTCCTCAGACTCCACG+CGG | + | chr6.1:77536382-77536401 | MS.gene061462:CDS | 55.0% | |
| TCTGACCCTGCTGAACCTCG+AGG | - | chr6.1:77536194-77536213 | None:intergenic | 60.0% | |
| CTCCTCAGACTCCACGCGGT+GGG | + | chr6.1:77536386-77536405 | MS.gene061462:CDS | 65.0% | |
| ACTCCACGCGGTGGGAGCCT+TGG | + | chr6.1:77536394-77536413 | MS.gene061462:CDS | 70.0% | |
| CCACCGCGTGGAGTCTGAGG+AGG | - | chr6.1:77536388-77536407 | None:intergenic | 70.0% | |
| CCTCCTCAGACTCCACGCGG+TGG | + | chr6.1:77536385-77536404 | MS.gene061462:CDS | 70.0% | |
| CTCCCACCGCGTGGAGTCTG+AGG | - | chr6.1:77536391-77536410 | None:intergenic | 70.0% | |
| TGTCCAAGGCTCCCACCGCG+TGG | - | chr6.1:77536400-77536419 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 77535860 | 77536435 | 77535860 | ID=MS.gene061462 |
| chr6.1 | mRNA | 77535860 | 77536435 | 77535860 | ID=MS.gene061462.t1;Parent=MS.gene061462 |
| chr6.1 | exon | 77535860 | 77536083 | 77535860 | ID=MS.gene061462.t1.exon1;Parent=MS.gene061462.t1 |
| chr6.1 | CDS | 77535860 | 77536083 | 77535860 | ID=cds.MS.gene061462.t1;Parent=MS.gene061462.t1 |
| chr6.1 | exon | 77536171 | 77536246 | 77536171 | ID=MS.gene061462.t1.exon2;Parent=MS.gene061462.t1 |
| chr6.1 | CDS | 77536171 | 77536246 | 77536171 | ID=cds.MS.gene061462.t1;Parent=MS.gene061462.t1 |
| chr6.1 | exon | 77536358 | 77536435 | 77536358 | ID=MS.gene061462.t1.exon3;Parent=MS.gene061462.t1 |
| chr6.1 | CDS | 77536358 | 77536435 | 77536358 | ID=cds.MS.gene061462.t1;Parent=MS.gene061462.t1 |
| Gene Sequence |
| Protein sequence |
