Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061681.t1 | KEH40763.1 | 93.1 | 72 | 5 | 0 | 1 | 72 | 1 | 72 | 8.70E-27 | 129 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061681.t1 | A0A072VH50 | 93.1 | 72 | 5 | 0 | 1 | 72 | 1 | 72 | 6.3e-27 | 129.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050780 | MS.gene061681 | 0.804487 | 2.11E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene061681 | 0.804539 | 2.06E-49 | -1.69E-46 |
| MS.gene056091 | MS.gene061681 | 0.825144 | 5.50E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061681.t1 | MTR_1g034290 | 93.056 | 72 | 5 | 0 | 1 | 72 | 1 | 72 | 3.68e-44 | 136 |
| MS.gene061681.t1 | MTR_1g034270 | 76.389 | 72 | 12 | 1 | 1 | 72 | 1 | 67 | 5.16e-34 | 110 |
| MS.gene061681.t1 | MTR_4g068560 | 50.667 | 75 | 31 | 3 | 1 | 71 | 1 | 73 | 5.31e-19 | 73.2 |
| MS.gene061681.t1 | MTR_4g068453 | 42.105 | 76 | 33 | 3 | 1 | 71 | 1 | 70 | 1.85e-12 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTTGGATTTGGAAAATT+TGG | 0.091946 | 1.3:-19021268 | None:intergenic |
| CTCTTCTTCTAAACCATCTT+TGG | 0.198131 | 1.3:-19021283 | None:intergenic |
| TTCTAAACCATCTTTGGATT+TGG | 0.216462 | 1.3:-19021277 | None:intergenic |
| GTCAACAATATGTTTGCTTA+TGG | 0.258869 | 1.3:+19021374 | MS.gene061681:CDS |
| TTAGAAGAAGAGAAGGAAGT+TGG | 0.379164 | 1.3:+19021294 | MS.gene061681:CDS |
| TAGAACTTTCTCTTTGAGCT+TGG | 0.434544 | 1.3:-19021235 | None:intergenic |
| AGTTGGAAGGGAAGCTCAAA+AGG | 0.457096 | 1.3:+19021311 | MS.gene061681:CDS |
| AGAAGAGAAGGAAGTTGGAA+GGG | 0.471300 | 1.3:+19021299 | MS.gene061681:CDS |
| AAGAAGAGAAGGAAGTTGGA+AGG | 0.492250 | 1.3:+19021298 | MS.gene061681:CDS |
| AGATGGTTTAGAAGAAGAGA+AGG | 0.510389 | 1.3:+19021287 | MS.gene061681:CDS |
| ACATATTGTTGACTCAGATA+AGG | 0.525899 | 1.3:-19021364 | None:intergenic |
| TGTCGGCCATGGTAGAGGTA+TGG | 0.538180 | 1.3:+19021199 | MS.gene061681:CDS |
| GCCATGGTAGAGGTATGGAT+TGG | 0.607798 | 1.3:+19021204 | MS.gene061681:CDS |
| ATAAACAACATATGTTAGCA+AGG | 0.613097 | 1.3:-19021408 | None:intergenic |
| ACCAATCCATACCTCTACCA+TGG | 0.656584 | 1.3:-19021205 | None:intergenic |
| TGGAAGGGAAGCTCAAAAGG+AGG | 0.704527 | 1.3:+19021314 | MS.gene061681:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCTTTGGATTTGGAAAATT+TGG | - | chr1.3:19021271-19021290 | None:intergenic | 25.0% |
| !! | AAATTTTCCAAATCCAAAGA+TGG | + | chr1.3:19021270-19021289 | MS.gene061681:CDS | 25.0% |
| !!! | TTTGGAAAATTTGGTTTTGT+TGG | - | chr1.3:19021262-19021281 | None:intergenic | 25.0% |
| GTCAACAATATGTTTGCTTA+TGG | + | chr1.3:19021374-19021393 | MS.gene061681:CDS | 30.0% | |
| TTCTAAACCATCTTTGGATT+TGG | - | chr1.3:19021280-19021299 | None:intergenic | 30.0% | |
| !! | ACATATTGTTGACTCAGATA+AGG | - | chr1.3:19021367-19021386 | None:intergenic | 30.0% |
| AGATGGTTTAGAAGAAGAGA+AGG | + | chr1.3:19021287-19021306 | MS.gene061681:CDS | 35.0% | |
| CTCTTCTTCTAAACCATCTT+TGG | - | chr1.3:19021286-19021305 | None:intergenic | 35.0% | |
| TAGAACTTTCTCTTTGAGCT+TGG | - | chr1.3:19021238-19021257 | None:intergenic | 35.0% | |
| TTAGAAGAAGAGAAGGAAGT+TGG | + | chr1.3:19021294-19021313 | MS.gene061681:CDS | 35.0% | |
| AAGAAGAGAAGGAAGTTGGA+AGG | + | chr1.3:19021298-19021317 | MS.gene061681:CDS | 40.0% | |
| AGAAGAGAAGGAAGTTGGAA+GGG | + | chr1.3:19021299-19021318 | MS.gene061681:CDS | 40.0% | |
| ACCAATCCATACCTCTACCA+TGG | - | chr1.3:19021208-19021227 | None:intergenic | 45.0% | |
| AGTTGGAAGGGAAGCTCAAA+AGG | + | chr1.3:19021311-19021330 | MS.gene061681:CDS | 45.0% | |
| TGGAAGGGAAGCTCAAAAGG+AGG | + | chr1.3:19021314-19021333 | MS.gene061681:CDS | 50.0% | |
| ! | GCCATGGTAGAGGTATGGAT+TGG | + | chr1.3:19021204-19021223 | MS.gene061681:CDS | 50.0% |
| TGTCGGCCATGGTAGAGGTA+TGG | + | chr1.3:19021199-19021218 | MS.gene061681:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 19021198 | 19021416 | 19021198 | ID=MS.gene061681 |
| chr1.3 | mRNA | 19021198 | 19021416 | 19021198 | ID=MS.gene061681.t1;Parent=MS.gene061681 |
| chr1.3 | exon | 19021198 | 19021416 | 19021198 | ID=MS.gene061681.t1.exon1;Parent=MS.gene061681.t1 |
| chr1.3 | CDS | 19021198 | 19021416 | 19021198 | ID=cds.MS.gene061681.t1;Parent=MS.gene061681.t1 |
| Gene Sequence |
| Protein sequence |
