AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061723

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061723.t1 MTR_5g014710 64.968 314 108 1 90 403 47 358 5.88e-146 419
MS.gene061723.t1 MTR_4g092690 65.079 315 108 1 89 403 44 356 1.51e-141 407
MS.gene061723.t1 MTR_8g463760 60.121 331 126 3 77 403 15 343 1.86e-139 402
MS.gene061723.t1 MTR_8g463760 63.547 203 71 1 77 276 15 217 3.66e-88 268
MS.gene061723.t1 MTR_2g021700 72.549 51 14 0 256 306 52 102 4.04e-16 75.5
MS.gene061723.t1 MTR_2g045010 72.549 51 14 0 256 306 52 102 4.04e-16 75.5
MS.gene061723.t1 MTR_4g033285 73.333 45 12 0 256 300 52 96 1.45e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061723.t1 AT3G47520 77.309 379 76 4 20 398 23 391 0.0 573
MS.gene061723.t1 AT1G53240 64.537 313 109 1 91 403 31 341 2.71e-144 414
MS.gene061723.t1 AT2G22780 63.609 327 117 1 77 403 30 354 1.13e-143 413
MS.gene061723.t1 AT3G15020 63.259 313 113 1 91 403 31 341 1.47e-140 404
MS.gene061723.t1 AT5G09660 60.870 345 129 3 63 403 12 354 1.64e-135 392
MS.gene061723.t1 AT5G09660 64.762 315 109 1 89 403 21 333 3.87e-135 390
MS.gene061723.t1 AT5G09660 60.580 345 129 4 63 403 12 353 3.54e-133 386
MS.gene061723.t1 AT3G15020 64.338 272 97 0 91 362 31 302 9.82e-125 363
MS.gene061723.t1 AT5G09660 63.322 289 102 2 63 347 12 300 6.41e-117 345
MS.gene061723.t1 AT5G09660 68.605 258 81 0 90 347 22 279 2.05e-116 343
MS.gene061723.t1 AT3G53910 49.438 89 45 0 318 406 9 97 2.55e-26 102

Find 110 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTCAATCTAGTCCTTT+TGG 0.231181 8.1:+16679980 MS.gene061723:CDS
CAACACCCCTTCGAAAGTTT+TGG 0.243573 8.1:+16680330 MS.gene061723:CDS
GAAACACCAAAGAGCTTCTT+AGG 0.295270 8.1:-16680604 None:intergenic
GCAAGTTGCGGGTGAGGTTT+CGG 0.332641 8.1:-16680153 None:intergenic
GAGGAAGTGACCTCCCTGTT+TGG 0.346298 8.1:-16679964 None:intergenic
GTTAATTTCAACACCTTCTC+TGG 0.351785 8.1:+16680022 MS.gene061723:CDS
ACTATTGGAACCACCCAAAC+AGG 0.361628 8.1:+16679950 MS.gene061723:CDS
AGCGGCACAAGTTTCTTTGC+CGG 0.363971 8.1:-16680092 None:intergenic
TCGATGCTGGCCTTAAGTTC+TGG 0.369041 8.1:-16681084 None:intergenic
AGGAAGTGACCTCCCTGTTT+GGG 0.375677 8.1:-16679963 None:intergenic
TCTCATCAAGATGTCGCCTT+TGG 0.378753 8.1:+16680243 MS.gene061723:CDS
CTTGGTGCTGCAGGAGGGAT+TGG 0.382446 8.1:+16680205 MS.gene061723:CDS
ATAATATGAATAAAAGCATT+GGG 0.384767 8.1:-16680514 None:intergenic
TGAGTATGAGCAGAAGGCTT+TGG 0.385556 8.1:+16681050 MS.gene061723:CDS
CGAGTCATGCCGGGCTTCCT+AGG 0.395727 8.1:-16680424 None:intergenic
TATGATATTGCGAATGTTAA+GGG 0.396701 8.1:+16680283 MS.gene061723:CDS
TTTAGAATTTCAGCGGCAAT+AGG 0.400849 8.1:-16680562 None:intergenic
CTATGATATTGCGAATGTTA+AGG 0.401378 8.1:+16680282 MS.gene061723:CDS
TCCTACAAAGTAGCGGTTCT+TGG 0.402994 8.1:+16680187 MS.gene061723:CDS
GTTGAGGCGAAGGCTGGTGC+AGG 0.406244 8.1:+16680829 MS.gene061723:CDS
TGCCTGAGGCTGCAAGTTGC+GGG 0.408008 8.1:-16680164 None:intergenic
TCGTCTCTTCGTGCACTTGA+CGG 0.411087 8.1:+16680898 MS.gene061723:CDS
CAGTTGTTGGAGGTCATGCC+GGG 0.417388 8.1:+16680701 MS.gene061723:CDS
ACCAAGAACCGCTACTTTGT+AGG 0.417991 8.1:-16680188 None:intergenic
CACCCGCAACTTGCAGCCTC+AGG 0.423294 8.1:+16680162 MS.gene061723:CDS
CCGGCATGACCTCCAACAAC+TGG 0.427912 8.1:-16680700 None:intergenic
ACATCAATGCTGGTATAGTC+AGG 0.428901 8.1:+16680461 MS.gene061723:CDS
AGCACTTAAGCCAGAACTTA+AGG 0.432437 8.1:+16681074 MS.gene061723:CDS
TGAGAGATGGCATGGCTTTG+AGG 0.432465 8.1:-16680045 None:intergenic
TATGATCCTAAGAAGCTCTT+TGG 0.434339 8.1:+16680598 MS.gene061723:CDS
AGGGTCTGCTACTTTGTCAA+TGG 0.435055 8.1:+16680849 MS.gene061723:CDS
GGCTTCCTAGGAACACCGGC+AGG 0.435127 8.1:-16680412 None:intergenic
GAGGCTGCAAGTTGCGGGTG+AGG 0.436560 8.1:-16680159 None:intergenic
GATGTGGATGTTCCAGTTGT+TGG 0.440014 8.1:+16680688 MS.gene061723:CDS
GTAGCGGTTCTTGGTGCTGC+AGG 0.442098 8.1:+16680196 MS.gene061723:CDS
CTCAATCCTTGGAGATCAGT+TGG 0.450054 8.1:-16681027 None:intergenic
GATAATATGAATAAAAGCAT+TGG 0.454366 8.1:-16680515 None:intergenic
CCAGTTGTTGGAGGTCATGC+CGG 0.456311 8.1:+16680700 MS.gene061723:CDS
TTATCCCTGCCGGTGTTCCT+AGG 0.457807 8.1:+16680407 MS.gene061723:CDS
TTTCACTGATGAAGAAATTG+AGG 0.470058 8.1:+16680768 MS.gene061723:CDS
TTTGCCGGAGAAAGATGATT+CGG 0.474830 8.1:-16680077 None:intergenic
ATGCCTGAGGCTGCAAGTTG+CGG 0.479068 8.1:-16680165 None:intergenic
GCCGGTGTTCCTAGGAAGCC+CGG 0.487133 8.1:+16680415 MS.gene061723:CDS
GCTCATACTCACTCAATCCT+TGG 0.489832 8.1:-16681038 None:intergenic
GAATTGAATTTCAAACCAAA+AGG 0.499197 8.1:-16679995 None:intergenic
TGGTTTGAAATTCAATTCCC+AGG 0.499844 8.1:+16680000 MS.gene061723:CDS
CTTCAAGGGTGAAGATTGGT+AGG 0.501008 8.1:+16680983 MS.gene061723:CDS
TACCAGCATTGATGTTGAAA+AGG 0.504347 8.1:-16680453 None:intergenic
TTTGCTTCAAGGGTGAAGAT+TGG 0.507205 8.1:+16680979 MS.gene061723:CDS
GAGTTAGCAAATTGTTTGAA+AGG 0.510601 8.1:+16680370 MS.gene061723:CDS
AAGGTCGTCACGAGTCATGC+CGG 0.512854 8.1:-16680434 None:intergenic
AAACCAAAAGGACTAGATTG+AGG 0.513013 8.1:-16679983 None:intergenic
TCGCAATATCATAGAGATGC+AGG 0.515907 8.1:-16680273 None:intergenic
AGCAAATTGTTTGAAAGGTG+TGG 0.519677 8.1:+16680375 MS.gene061723:CDS
ATAGGAACTGTAGAGTTCAC+TGG 0.520893 8.1:-16680544 None:intergenic
CGCTGAAATTCTAAAACAGA+AGG 0.523131 8.1:+16680570 MS.gene061723:CDS
AGGAGGGATTGGTCAGCCAC+TGG 0.524866 8.1:+16680216 MS.gene061723:CDS
TGCTGGTATAGTCAGGGACT+TGG 0.529987 8.1:+16680468 MS.gene061723:CDS
GTGGATGTTCCAGTTGTTGG+AGG 0.532818 8.1:+16680691 MS.gene061723:CDS
GCGGTTCTTGGTGCTGCAGG+AGG 0.533048 8.1:+16680199 MS.gene061723:CDS
GTGAAATCCAAAACTTTCGA+AGG 0.537192 8.1:-16680337 None:intergenic
ACTGTCAGGATTCAAAATGC+TGG 0.538512 8.1:+16680796 MS.gene061723:CDS
AGGTCGTCACGAGTCATGCC+GGG 0.542713 8.1:-16680433 None:intergenic
AGGGTGAAGATTGGTAGGAA+AGG 0.542764 8.1:+16680988 MS.gene061723:CDS
AAGTGACCTCCCTGTTTGGG+TGG 0.544528 8.1:-16679960 None:intergenic
GAGAGGAAGAATTGTAATCC+CGG 0.547677 8.1:-16680719 None:intergenic
GACTCCGAATCATCTTTCTC+CGG 0.550000 8.1:+16680073 MS.gene061723:CDS
AGAAGGTGTTGAAATTAACC+TGG 0.550640 8.1:-16680018 None:intergenic
TTTCTACACTTGATGTTGTG+AGG 0.552915 8.1:+16680623 MS.gene061723:CDS
GAGGTTGTTGAGGCGAAGGC+TGG 0.553690 8.1:+16680823 MS.gene061723:CDS
ATTGAGTGAGTATGAGCAGA+AGG 0.555968 8.1:+16681044 MS.gene061723:CDS
AACTGAGGTTGTTGAGGCGA+AGG 0.556107 8.1:+16680819 MS.gene061723:CDS
TTAATTCCAACTGATCTCCA+AGG 0.558509 8.1:+16681021 MS.gene061723:CDS
CTTAAGGCCAGCATCGAGAA+GGG 0.563554 8.1:+16681090 MS.gene061723:CDS
TCATCAGTGAAACTCACTGA+GGG 0.567339 8.1:-16680757 None:intergenic
GATGTTGTTGTTATCCCTGC+CGG 0.571032 8.1:+16680397 MS.gene061723:CDS
AAATTGAGGAGCTAACTGTC+AGG 0.577890 8.1:+16680782 MS.gene061723:CDS
GCCGGGCTTCCTAGGAACAC+CGG 0.580002 8.1:-16680416 None:intergenic
ACTTAAGGCCAGCATCGAGA+AGG 0.580005 8.1:+16681089 MS.gene061723:CDS
GATTCAAAATGCTGGAACTG+AGG 0.580164 8.1:+16680804 MS.gene061723:CDS
CGGTTCTTGGTGCTGCAGGA+GGG 0.586824 8.1:+16680200 MS.gene061723:CDS
GAACCTGAGGTTGATTGATG+TGG 0.587092 8.1:+16680672 MS.gene061723:CDS
TTCTACACTTGATGTTGTGA+GGG 0.588106 8.1:+16680624 MS.gene061723:CDS
CATCCACATCAATCAACCTC+AGG 0.589306 8.1:-16680675 None:intergenic
TGAAATCCAAAACTTTCGAA+GGG 0.589668 8.1:-16680336 None:intergenic
GTCACATCTGAGAGATGGCA+TGG 0.591020 8.1:-16680053 None:intergenic
CATGGCTTTGAGGCCAGAGA+AGG 0.592005 8.1:-16680035 None:intergenic
TCGGAGTCACATCTGAGAGA+TGG 0.601214 8.1:-16680058 None:intergenic
TTGAGGCGAAGGCTGGTGCA+GGG 0.614890 8.1:+16680830 MS.gene061723:CDS
TGCTGGAACTGAGGTTGTTG+AGG 0.616411 8.1:+16680813 MS.gene061723:CDS
ATTGGGGCAATTATCTGCAA+CGG 0.623966 8.1:-16680497 None:intergenic
TTGCTCAGAAGAAGAACCTG+AGG 0.625392 8.1:+16680659 MS.gene061723:CDS
CATCAATGCTGGTATAGTCA+GGG 0.635474 8.1:+16680462 MS.gene061723:CDS
GCTTCCTAGGAACACCGGCA+GGG 0.635800 8.1:-16680411 None:intergenic
CTATTGGAACCACCCAAACA+GGG 0.635885 8.1:+16679951 MS.gene061723:CDS
AGATGCAGGTCGGAAACCAA+AGG 0.636274 8.1:-16680259 None:intergenic
TGCAAAAGTTGCACGCAGAG+CGG 0.636866 8.1:-16680110 None:intergenic
GAAGGTGTTGAAATTAACCT+GGG 0.642084 8.1:-16680017 None:intergenic
GCTGAAATTCTAAAACAGAA+GGG 0.647110 8.1:+16680571 MS.gene061723:CDS
TTAAGGCCAGCATCGAGAAG+GGG 0.649629 8.1:+16681091 MS.gene061723:CDS
TAAGGCCAGCATCGAGAAGG+GGG 0.655068 8.1:+16681092 MS.gene061723:CDS
TAATATGAATAAAAGCATTG+GGG 0.668981 8.1:-16680513 None:intergenic
GATTGGTAGGAAAGGAGTCG+AGG 0.670539 8.1:+16680996 MS.gene061723:CDS
TTCATCAGTGAAACTCACTG+AGG 0.681230 8.1:-16680758 None:intergenic
TCAGGCATCCTACAAAGTAG+CGG 0.690931 8.1:+16680180 MS.gene061723:CDS
GAAATCCAAAACTTTCGAAG+GGG 0.694170 8.1:-16680335 None:intergenic
AATATCATAGAGATGCAGGT+CGG 0.697174 8.1:-16680269 None:intergenic
GCTACTTTGTAGGATGCCTG+AGG 0.706674 8.1:-16680178 None:intergenic
ATCTTGATGAGAAGTGCCAG+TGG 0.724135 8.1:-16680232 None:intergenic
TTGGAACCACCCAAACAGGG+AGG 0.754881 8.1:+16679954 MS.gene061723:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATGAATAAAAGCATT+GGG - chr8.1:16680517-16680536 None:intergenic 15.0%
!!! GATAATATGAATAAAAGCAT+TGG - chr8.1:16680518-16680537 None:intergenic 20.0%
!!! TAATATGAATAAAAGCATTG+GGG - chr8.1:16680516-16680535 None:intergenic 20.0%
! GAATTGAATTTCAAACCAAA+AGG - chr8.1:16679998-16680017 None:intergenic 25.0%
! TATGATATTGCGAATGTTAA+GGG + chr8.1:16680283-16680302 MS.gene061723:CDS 25.0%
CTATGATATTGCGAATGTTA+AGG + chr8.1:16680282-16680301 MS.gene061723:CDS 30.0%
GAGTTAGCAAATTGTTTGAA+AGG + chr8.1:16680370-16680389 MS.gene061723:CDS 30.0%
TGAAATCCAAAACTTTCGAA+GGG - chr8.1:16680339-16680358 None:intergenic 30.0%
TTTCACTGATGAAGAAATTG+AGG + chr8.1:16680768-16680787 MS.gene061723:CDS 30.0%
!! GCTGAAATTCTAAAACAGAA+GGG + chr8.1:16680571-16680590 MS.gene061723:CDS 30.0%
!!! CTTCTGTTTTAGAATTTCAG+CGG - chr8.1:16680572-16680591 None:intergenic 30.0%
AAACCAAAAGGACTAGATTG+AGG - chr8.1:16679986-16680005 None:intergenic 35.0%
AATATCATAGAGATGCAGGT+CGG - chr8.1:16680272-16680291 None:intergenic 35.0%
AGCAAATTGTTTGAAAGGTG+TGG + chr8.1:16680375-16680394 MS.gene061723:CDS 35.0%
GAAATCCAAAACTTTCGAAG+GGG - chr8.1:16680338-16680357 None:intergenic 35.0%
GTGAAATCCAAAACTTTCGA+AGG - chr8.1:16680340-16680359 None:intergenic 35.0%
GTTAATTTCAACACCTTCTC+TGG + chr8.1:16680022-16680041 MS.gene061723:CDS 35.0%
TATGATCCTAAGAAGCTCTT+TGG + chr8.1:16680598-16680617 MS.gene061723:CDS 35.0%
TTAATTCCAACTGATCTCCA+AGG + chr8.1:16681021-16681040 MS.gene061723:CDS 35.0%
TTCACCCTTGAAGCAAAAAA+CGG - chr8.1:16680976-16680995 None:intergenic 35.0%
TTCTACACTTGATGTTGTGA+GGG + chr8.1:16680624-16680643 MS.gene061723:CDS 35.0%
TTTCTACACTTGATGTTGTG+AGG + chr8.1:16680623-16680642 MS.gene061723:CDS 35.0%
! TGGTTTGAAATTCAATTCCC+AGG + chr8.1:16680000-16680019 MS.gene061723:CDS 35.0%
! TTTAGAATTTCAGCGGCAAT+AGG - chr8.1:16680565-16680584 None:intergenic 35.0%
!! AGAAGGTGTTGAAATTAACC+TGG - chr8.1:16680021-16680040 None:intergenic 35.0%
!! CGCTGAAATTCTAAAACAGA+AGG + chr8.1:16680570-16680589 MS.gene061723:CDS 35.0%
!! GAAGGTGTTGAAATTAACCT+GGG - chr8.1:16680020-16680039 None:intergenic 35.0%
!! TACCAGCATTGATGTTGAAA+AGG - chr8.1:16680456-16680475 None:intergenic 35.0%
!!! GCATCAGCTACTTTTACTAT+TGG + chr8.1:16679935-16679954 MS.gene061723:CDS 35.0%
!!! TCGAAAGTTTTGGATTTCAC+AGG + chr8.1:16680340-16680359 MS.gene061723:CDS 35.0%
AAATTGAGGAGCTAACTGTC+AGG + chr8.1:16680782-16680801 MS.gene061723:CDS 40.0%
ACATCAATGCTGGTATAGTC+AGG + chr8.1:16680461-16680480 MS.gene061723:CDS 40.0%
ACTGTCAGGATTCAAAATGC+TGG + chr8.1:16680796-16680815 MS.gene061723:CDS 40.0%
AGCACTTAAGCCAGAACTTA+AGG + chr8.1:16681074-16681093 MS.gene061723:CDS 40.0%
ATAGGAACTGTAGAGTTCAC+TGG - chr8.1:16680547-16680566 None:intergenic 40.0%
ATTGAGTGAGTATGAGCAGA+AGG + chr8.1:16681044-16681063 MS.gene061723:CDS 40.0%
ATTGGGGCAATTATCTGCAA+CGG - chr8.1:16680500-16680519 None:intergenic 40.0%
CATCAATGCTGGTATAGTCA+GGG + chr8.1:16680462-16680481 MS.gene061723:CDS 40.0%
GAAACACCAAAGAGCTTCTT+AGG - chr8.1:16680607-16680626 None:intergenic 40.0%
GAGAGGAAGAATTGTAATCC+CGG - chr8.1:16680722-16680741 None:intergenic 40.0%
GATTCAAAATGCTGGAACTG+AGG + chr8.1:16680804-16680823 MS.gene061723:CDS 40.0%
TCATCAGTGAAACTCACTGA+GGG - chr8.1:16680760-16680779 None:intergenic 40.0%
TCGCAATATCATAGAGATGC+AGG - chr8.1:16680276-16680295 None:intergenic 40.0%
TGAAGCAAAAAACGGAAGGT+CGG - chr8.1:16680968-16680987 None:intergenic 40.0%
TTCATCAGTGAAACTCACTG+AGG - chr8.1:16680761-16680780 None:intergenic 40.0%
TTTGCCGGAGAAAGATGATT+CGG - chr8.1:16680080-16680099 None:intergenic 40.0%
TTTGCTTCAAGGGTGAAGAT+TGG + chr8.1:16680979-16680998 MS.gene061723:CDS 40.0%
! CGGTTTTCCTTTTGAGCTTT+GGG - chr8.1:16680136-16680155 None:intergenic 40.0%
! CTTCCTCAATCTAGTCCTTT+TGG + chr8.1:16679980-16679999 MS.gene061723:CDS 40.0%
! GACCTTTTCAACATCAATGC+TGG + chr8.1:16680451-16680470 MS.gene061723:CDS 40.0%
! TCGGTTTTCCTTTTGAGCTT+TGG - chr8.1:16680137-16680156 None:intergenic 40.0%
! TTTTGCACCCAAAGCTCAAA+AGG + chr8.1:16680126-16680145 MS.gene061723:CDS 40.0%
!! GGTTTTGTCTTTGACAAGAG+AGG - chr8.1:16680739-16680758 None:intergenic 40.0%
!!! ACCTTCCGTTTTTTGCTTCA+AGG + chr8.1:16680968-16680987 MS.gene061723:CDS 40.0%
!!! CCTTCCGTTTTTTGCTTCAA+GGG + chr8.1:16680969-16680988 MS.gene061723:CDS 40.0%
ACTATTGGAACCACCCAAAC+AGG + chr8.1:16679950-16679969 MS.gene061723:CDS 45.0%
AGGGTGAAGATTGGTAGGAA+AGG + chr8.1:16680988-16681007 MS.gene061723:CDS 45.0%
CATCCACATCAATCAACCTC+AGG - chr8.1:16680678-16680697 None:intergenic 45.0%
CCCTTGAAGCAAAAAACGGA+AGG - chr8.1:16680972-16680991 None:intergenic 45.0%
CTATTGGAACCACCCAAACA+GGG + chr8.1:16679951-16679970 MS.gene061723:CDS 45.0%
CTCAATCCTTGGAGATCAGT+TGG - chr8.1:16681030-16681049 None:intergenic 45.0%
CTTCAAGGGTGAAGATTGGT+AGG + chr8.1:16680983-16681002 MS.gene061723:CDS 45.0%
GACTCCGAATCATCTTTCTC+CGG + chr8.1:16680073-16680092 MS.gene061723:CDS 45.0%
GATGTGGATGTTCCAGTTGT+TGG + chr8.1:16680688-16680707 MS.gene061723:CDS 45.0%
GCTCATACTCACTCAATCCT+TGG - chr8.1:16681041-16681060 None:intergenic 45.0%
TCAGGCATCCTACAAAGTAG+CGG + chr8.1:16680180-16680199 MS.gene061723:CDS 45.0%
TCCTACAAAGTAGCGGTTCT+TGG + chr8.1:16680187-16680206 MS.gene061723:CDS 45.0%
TCTCATCAAGATGTCGCCTT+TGG + chr8.1:16680243-16680262 MS.gene061723:CDS 45.0%
TGAGTATGAGCAGAAGGCTT+TGG + chr8.1:16681050-16681069 MS.gene061723:CDS 45.0%
TTGCTCAGAAGAAGAACCTG+AGG + chr8.1:16680659-16680678 MS.gene061723:CDS 45.0%
! ACCAAGAACCGCTACTTTGT+AGG - chr8.1:16680191-16680210 None:intergenic 45.0%
! CAACACCCCTTCGAAAGTTT+TGG + chr8.1:16680330-16680349 MS.gene061723:CDS 45.0%
! GAACCTGAGGTTGATTGATG+TGG + chr8.1:16680672-16680691 MS.gene061723:CDS 45.0%
! GATGTTGTTGTTATCCCTGC+CGG + chr8.1:16680397-16680416 MS.gene061723:CDS 45.0%
!! AGGGTCTGCTACTTTGTCAA+TGG + chr8.1:16680849-16680868 MS.gene061723:CDS 45.0%
!! ATCTTGATGAGAAGTGCCAG+TGG - chr8.1:16680235-16680254 None:intergenic 45.0%
AACTGAGGTTGTTGAGGCGA+AGG + chr8.1:16680819-16680838 MS.gene061723:CDS 50.0%
AGATGCAGGTCGGAAACCAA+AGG - chr8.1:16680262-16680281 None:intergenic 50.0%
AGCGGCACAAGTTTCTTTGC+CGG - chr8.1:16680095-16680114 None:intergenic 50.0%
AGGAAGTGACCTCCCTGTTT+GGG - chr8.1:16679966-16679985 None:intergenic 50.0%
GATTGGTAGGAAAGGAGTCG+AGG + chr8.1:16680996-16681015 MS.gene061723:CDS 50.0%
GTCACATCTGAGAGATGGCA+TGG - chr8.1:16680056-16680075 None:intergenic 50.0%
GTGGATGTTCCAGTTGTTGG+AGG + chr8.1:16680691-16680710 MS.gene061723:CDS 50.0%
TCGGAGTCACATCTGAGAGA+TGG - chr8.1:16680061-16680080 None:intergenic 50.0%
TCGTCTCTTCGTGCACTTGA+CGG + chr8.1:16680898-16680917 MS.gene061723:CDS 50.0%
TGCAAAAGTTGCACGCAGAG+CGG - chr8.1:16680113-16680132 None:intergenic 50.0%
TGCTGGAACTGAGGTTGTTG+AGG + chr8.1:16680813-16680832 MS.gene061723:CDS 50.0%
TGCTGGTATAGTCAGGGACT+TGG + chr8.1:16680468-16680487 MS.gene061723:CDS 50.0%
! ACTTAAGGCCAGCATCGAGA+AGG + chr8.1:16681089-16681108 MS.gene061723:CDS 50.0%
! CTTAAGGCCAGCATCGAGAA+GGG + chr8.1:16681090-16681109 MS.gene061723:CDS 50.0%
! GCTACTTTGTAGGATGCCTG+AGG - chr8.1:16680181-16680200 None:intergenic 50.0%
! TGAGAGATGGCATGGCTTTG+AGG - chr8.1:16680048-16680067 None:intergenic 50.0%
! TTAAGGCCAGCATCGAGAAG+GGG + chr8.1:16681091-16681110 MS.gene061723:CDS 50.0%
!! TCGATGCTGGCCTTAAGTTC+TGG - chr8.1:16681087-16681106 None:intergenic 50.0%
AAGGTCGTCACGAGTCATGC+CGG - chr8.1:16680437-16680456 None:intergenic 55.0%
AAGTGACCTCCCTGTTTGGG+TGG - chr8.1:16679963-16679982 None:intergenic 55.0%
ATGCCTGAGGCTGCAAGTTG+CGG - chr8.1:16680168-16680187 None:intergenic 55.0%
CAGTTGTTGGAGGTCATGCC+GGG + chr8.1:16680701-16680720 MS.gene061723:CDS 55.0%
CCAGTTGTTGGAGGTCATGC+CGG + chr8.1:16680700-16680719 MS.gene061723:CDS 55.0%
GAGGAAGTGACCTCCCTGTT+TGG - chr8.1:16679967-16679986 None:intergenic 55.0%
GCAAGTTGCGGGTGAGGTTT+CGG - chr8.1:16680156-16680175 None:intergenic 55.0%
TTATCCCTGCCGGTGTTCCT+AGG + chr8.1:16680407-16680426 MS.gene061723:CDS 55.0%
TTGGAACCACCCAAACAGGG+AGG + chr8.1:16679954-16679973 MS.gene061723:CDS 55.0%
! TAAGGCCAGCATCGAGAAGG+GGG + chr8.1:16681092-16681111 MS.gene061723:CDS 55.0%
!! CATGGCTTTGAGGCCAGAGA+AGG - chr8.1:16680038-16680057 None:intergenic 55.0%
AGCAACCCCCTTCTCGATGC+TGG - chr8.1:16681100-16681119 None:intergenic 60.0%
AGGTCGTCACGAGTCATGCC+GGG - chr8.1:16680436-16680455 None:intergenic 60.0%
CCGGCATGACCTCCAACAAC+TGG - chr8.1:16680703-16680722 None:intergenic 60.0%
GAGGTTGTTGAGGCGAAGGC+TGG + chr8.1:16680823-16680842 MS.gene061723:CDS 60.0%
GCTTCCTAGGAACACCGGCA+GGG - chr8.1:16680414-16680433 None:intergenic 60.0%
TGCCTGAGGCTGCAAGTTGC+GGG - chr8.1:16680167-16680186 None:intergenic 60.0%
TTGAGGCGAAGGCTGGTGCA+GGG + chr8.1:16680830-16680849 MS.gene061723:CDS 60.0%
!! AGGAGGGATTGGTCAGCCAC+TGG + chr8.1:16680216-16680235 MS.gene061723:CDS 60.0%
!! CGGTTCTTGGTGCTGCAGGA+GGG + chr8.1:16680200-16680219 MS.gene061723:CDS 60.0%
!! CTTGGTGCTGCAGGAGGGAT+TGG + chr8.1:16680205-16680224 MS.gene061723:CDS 60.0%
!! GTAGCGGTTCTTGGTGCTGC+AGG + chr8.1:16680196-16680215 MS.gene061723:CDS 60.0%
CACCCGCAACTTGCAGCCTC+AGG + chr8.1:16680162-16680181 MS.gene061723:CDS 65.0%
CGAGTCATGCCGGGCTTCCT+AGG - chr8.1:16680427-16680446 None:intergenic 65.0%
GAGGCTGCAAGTTGCGGGTG+AGG - chr8.1:16680162-16680181 None:intergenic 65.0%
GCCGGGCTTCCTAGGAACAC+CGG - chr8.1:16680419-16680438 None:intergenic 65.0%
GCCGGTGTTCCTAGGAAGCC+CGG + chr8.1:16680415-16680434 MS.gene061723:CDS 65.0%
GGCTTCCTAGGAACACCGGC+AGG - chr8.1:16680415-16680434 None:intergenic 65.0%
GTTGAGGCGAAGGCTGGTGC+AGG + chr8.1:16680829-16680848 MS.gene061723:CDS 65.0%
!! GCGGTTCTTGGTGCTGCAGG+AGG + chr8.1:16680199-16680218 MS.gene061723:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 16679923 16681149 16679923 ID=MS.gene061723
chr8.1 mRNA 16679923 16681149 16679923 ID=MS.gene061723.t1;Parent=MS.gene061723
chr8.1 exon 16679923 16681149 16679923 ID=MS.gene061723.t1.exon1;Parent=MS.gene061723.t1
chr8.1 CDS 16679923 16681149 16679923 ID=cds.MS.gene061723.t1;Parent=MS.gene061723.t1
Gene Sequence

>MS.gene061723

ATGGCGGCAGCAGCATCAGCTACTTTTACTATTGGAACCACCCAAACAGGGAGGTCACTTCCTCAATCTAGTCCTTTTGGTTTGAAATTCAATTCCCAGGTTAATTTCAACACCTTCTCTGGCCTCAAAGCCATGCCATCTCTCAGATGTGACTCCGAATCATCTTTCTCCGGCAAAGAAACTTGTGCCGCTCTGCGTGCAACTTTTGCACCCAAAGCTCAAAAGGAAAACCGAAACCTCACCCGCAACTTGCAGCCTCAGGCATCCTACAAAGTAGCGGTTCTTGGTGCTGCAGGAGGGATTGGTCAGCCACTGGCACTTCTCATCAAGATGTCGCCTTTGGTTTCCGACCTGCATCTCTATGATATTGCGAATGTTAAGGGAGTTGCTGCTGATATCAGTCATTGCAACACCCCTTCGAAAGTTTTGGATTTCACAGGTGCTTCTGAGTTAGCAAATTGTTTGAAAGGTGTGGATGTTGTTGTTATCCCTGCCGGTGTTCCTAGGAAGCCCGGCATGACTCGTGACGACCTTTTCAACATCAATGCTGGTATAGTCAGGGACTTGGTCACTGCCGTTGCAGATAATTGCCCCAATGCTTTTATTCATATTATCAGTAATCCAGTGAACTCTACAGTTCCTATTGCCGCTGAAATTCTAAAACAGAAGGGTGTCTATGATCCTAAGAAGCTCTTTGGTGTTTCTACACTTGATGTTGTGAGGGCAAACACATTTGTTGCTCAGAAGAAGAACCTGAGGTTGATTGATGTGGATGTTCCAGTTGTTGGAGGTCATGCCGGGATTACAATTCTTCCTCTCTTGTCAAAGACAAAACCCTCAGTGAGTTTCACTGATGAAGAAATTGAGGAGCTAACTGTCAGGATTCAAAATGCTGGAACTGAGGTTGTTGAGGCGAAGGCTGGTGCAGGGTCTGCTACTTTGTCAATGGCTTATGCAGCAGCAAGATTTGTTGAATCGTCTCTTCGTGCACTTGACGGAGACGCTGATGTGTATGAGTGCTCATTCGTACAGTCAGATCTGACCGACCTTCCGTTTTTTGCTTCAAGGGTGAAGATTGGTAGGAAAGGAGTCGAGGCTTTAATTCCAACTGATCTCCAAGGATTGAGTGAGTATGAGCAGAAGGCTTTGGAAGCACTTAAGCCAGAACTTAAGGCCAGCATCGAGAAGGGGGTTGCTTTTGCTCAAAAGCAAACTGTTACTGCTTAG
Protein sequence

>MS.gene061723.t1

MAAAASATFTIGTTQTGRSLPQSSPFGLKFNSQVNFNTFSGLKAMPSLRCDSESSFSGKETCAALRATFAPKAQKENRNLTRNLQPQASYKVAVLGAAGGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPSKVLDFTGASELANCLKGVDVVVIPAGVPRKPGMTRDDLFNINAGIVRDLVTAVADNCPNAFIHIISNPVNSTVPIAAEILKQKGVYDPKKLFGVSTLDVVRANTFVAQKKNLRLIDVDVPVVGGHAGITILPLLSKTKPSVSFTDEEIEELTVRIQNAGTEVVEAKAGAGSATLSMAYAAARFVESSLRALDGDADVYECSFVQSDLTDLPFFASRVKIGRKGVEALIPTDLQGLSEYEQKALEALKPELKASIEKGVAFAQKQTVTA