AlfalfaGEDB | MS.gene061959

M. sativa cultivar XinJiangDaYe / MS.gene061959

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0008270 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043167 GO:0043169 GO:0043565 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00320.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061959.t1	XP_003619010.2	87.5	200	13	2	1	200	74	261	1.50E-89	339

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061959.t1	O49743	80.9	68	13	0	130	197	157	224	1.7e-28	127.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061959.t1	G7KQ55	87.5	200	13	2	1	200	74	261	1.1e-89	339.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene061959.t1	TF	C2C2-GATA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053488	MS.gene061959	-0.803042	4.21E-49	-1.69E-46
MS.gene058840	MS.gene061959	-0.804805	1.81E-49	-1.69E-46
MS.gene06074	MS.gene061959	-0.815311	9.87E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061959.t1	MTR_6g032830	91.000	200	6	2	1	200	74	261	8.40e-131	368
MS.gene061959.t1	MTR_7g085410	57.561	205	49	6	1	199	84	256	7.65e-69	211
MS.gene061959.t1	MTR_1g094930	80.000	65	13	0	131	195	147	211	1.27e-34	122
MS.gene061959.t1	MTR_4g071590	81.538	65	12	0	131	195	211	275	1.61e-34	124
MS.gene061959.t1	MTR_4g471590	81.538	65	12	0	131	195	211	275	1.61e-34	124
MS.gene061959.t1	MTR_1g094930	80.000	65	13	0	131	195	242	306	4.91e-34	123
MS.gene061959.t1	MTR_1g006650	77.612	67	15	0	131	197	234	300	9.56e-34	123
MS.gene061959.t1	MTR_2g094600	77.143	70	16	0	131	200	216	285	1.06e-33	122
MS.gene061959.t1	MTR_8g076340	73.333	75	18	1	121	195	210	282	1.40e-33	121
MS.gene061959.t1	MTR_8g013950	73.913	69	18	0	129	197	224	292	2.81e-33	122
MS.gene061959.t1	MTR_1g116010	70.270	74	22	0	123	196	156	229	4.52e-33	119
MS.gene061959.t1	MTR_3g109760	68.750	80	20	1	118	197	199	273	5.46e-33	120
MS.gene061959.t1	MTR_3g117380	78.462	65	14	0	131	195	229	293	1.99e-32	119
MS.gene061959.t1	MTR_1g046680	76.471	68	16	0	130	197	171	238	3.31e-32	116
MS.gene061959.t1	MTR_5g021340	80.000	65	13	0	131	195	300	364	3.71e-32	119
MS.gene061959.t1	MTR_4g058910	76.923	65	15	0	131	195	314	378	4.91e-31	117
MS.gene061959.t1	MTR_5g007600	72.059	68	19	0	130	197	258	325	5.32e-31	115
MS.gene061959.t1	MTR_5g007600	68.657	67	21	0	130	196	156	222	8.43e-29	109
MS.gene061959.t1	MTR_7g446110	57.576	66	28	0	127	192	183	248	4.26e-21	87.8
MS.gene061959.t1	MTR_7g445950	56.452	62	27	0	131	192	120	181	3.85e-19	81.3
MS.gene061959.t1	MTR_7g445980	58.730	63	25	1	131	192	33	95	8.49e-19	77.8
MS.gene061959.t1	MTR_7g446070	54.839	62	28	0	131	192	132	193	1.21e-18	80.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061959.t1	AT4G32890	45.652	184	44	8	14	197	136	263	5.37e-37	130
MS.gene061959.t1	AT3G60530	79.710	69	14	0	129	197	156	224	2.65e-36	127
MS.gene061959.t1	AT2G45050	41.436	181	60	3	17	197	111	245	1.55e-35	125
MS.gene061959.t1	AT5G25830	38.265	196	90	5	2	197	121	285	5.84e-35	126
MS.gene061959.t1	AT3G24050	75.000	68	17	0	130	197	193	260	2.40e-34	123
MS.gene061959.t1	AT5G66320	63.953	86	31	0	110	195	228	313	6.18e-34	123
MS.gene061959.t1	AT5G66320	63.953	86	31	0	110	195	228	313	6.18e-34	123
MS.gene061959.t1	AT3G54810	67.470	83	26	1	113	195	212	293	3.80e-33	121
MS.gene061959.t1	AT3G54810	67.470	83	26	1	113	195	212	293	3.80e-33	121
MS.gene061959.t1	AT3G51080	81.538	65	12	0	131	195	221	285	5.77e-33	120
MS.gene061959.t1	AT4G36240	72.464	69	19	0	131	199	164	232	6.88e-33	118
MS.gene061959.t1	AT1G08010	71.212	66	19	0	130	195	219	284	2.14e-32	119
MS.gene061959.t1	AT1G08010	71.212	66	19	0	130	195	219	284	2.14e-32	119
MS.gene061959.t1	AT1G08010	71.212	66	19	0	130	195	219	284	2.14e-32	119
MS.gene061959.t1	AT4G34680	73.846	65	17	0	131	195	180	244	2.15e-31	115
MS.gene061959.t1	AT4G34680	73.846	65	17	0	131	195	180	244	2.15e-31	115
MS.gene061959.t1	AT1G08000	57.778	90	34	1	106	195	197	282	2.41e-31	116
MS.gene061959.t1	AT1G08000	57.778	90	34	1	106	195	197	282	2.41e-31	116
MS.gene061959.t1	AT2G28340	42.105	133	71	2	68	197	128	257	1.52e-27	105
MS.gene061959.t1	AT3G45170	61.538	65	25	0	133	197	117	181	4.81e-26	99.8
MS.gene061959.t1	AT5G49300	46.296	54	22	1	113	166	25	71	6.09e-11	58.5

Find 34 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTGTTGAAACAAGCTTAT+TGG	0.158008	6.2:+20951405	MS.gene061959:CDS
CAGCTCCTAGTACCAAAATT+TGG	0.286985	6.2:+20951336	MS.gene061959:CDS
ATGTGAATGTGACCAAATTT+TGG	0.288556	6.2:-20951348	None:intergenic
CATGCATTGCATAGTGTCTT+TGG	0.309772	6.2:-20951701	None:intergenic
ATGGTCCTGCCCTCCATTGT+GGG	0.318440	6.2:-20951673	None:intergenic
TGAGTGCACCTTCTTGCATT+TGG	0.340927	6.2:-20951632	None:intergenic
ACAATGGAGGGCAGGACCAT+TGG	0.400380	6.2:+20951676	MS.gene061959:CDS
AATGGTCCTGCCCTCCATTG+TGG	0.428822	6.2:-20951674	None:intergenic
GAGAATGCAATCTATTAAGT+AGG	0.465726	6.2:+20951832	MS.gene061959:CDS
CAATGGAGGGCAGGACCATT+GGG	0.466961	6.2:+20951677	MS.gene061959:CDS
CGGAGCAATGAGTTCGCTGT+CGG	0.489222	6.2:-20951432	None:intergenic
GTAGGTTGTTGCCAGAGTAT+AGG	0.492955	6.2:+20951744	MS.gene061959:CDS
CATAGTGTCTTTGGTCCCAA+TGG	0.499888	6.2:-20951692	None:intergenic
TGCAAGTAGCTAACAAAAGT+AGG	0.538933	6.2:-20951779	None:intergenic
CTCTTCGCTGGCCTATACTC+TGG	0.539921	6.2:-20951755	None:intergenic
AGGACCCCACAATGGAGGGC+AGG	0.548944	6.2:+20951668	MS.gene061959:CDS
TGGTCCTGCCCTCCATTGTG+GGG	0.559124	6.2:-20951672	None:intergenic
AACATATTCCAAATGCAAGA+AGG	0.563153	6.2:+20951624	MS.gene061959:CDS
AAGCTTGTTTCAACAAAGGA+GGG	0.570788	6.2:-20951400	None:intergenic
AGAATGCAATCTATTAAGTA+GGG	0.572327	6.2:+20951833	MS.gene061959:CDS
TAAGCTTGTTTCAACAAAGG+AGG	0.573541	6.2:-20951401	None:intergenic
CATTCACATCTTATAAGTGA+TGG	0.584037	6.2:+20951362	MS.gene061959:CDS
TGTGGTGTGAGGTACAAGTC+TGG	0.585109	6.2:+20951722	MS.gene061959:CDS
CAATAAGCTTGTTTCAACAA+AGG	0.588114	6.2:-20951404	None:intergenic
ACAAAGGACCCCACAATGGA+GGG	0.595325	6.2:+20951664	MS.gene061959:CDS
GTGTGAGGTACAAGTCTGGT+AGG	0.597780	6.2:+20951726	MS.gene061959:CDS
GCACTCATTGTTTGTCACAA+AGG	0.603068	6.2:+20951648	MS.gene061959:CDS
CACAAAGGACCCCACAATGG+AGG	0.605327	6.2:+20951663	MS.gene061959:CDS
ACAAAAGTAGGACTCTTCGC+TGG	0.624466	6.2:-20951767	None:intergenic
AAAGTCTCTGCAGTTGCGTG+CGG	0.637759	6.2:+20951467	MS.gene061959:CDS
TGATGTTAATGATGATGATG+AGG	0.646926	6.2:+20951601	MS.gene061959:CDS
TGTCACAAAGGACCCCACAA+TGG	0.681006	6.2:+20951660	MS.gene061959:CDS
TATGCAATGCATGTGGTGTG+AGG	0.734829	6.2:+20951711	MS.gene061959:CDS
AAGACACTATGCAATGCATG+TGG	0.764250	6.2:+20951704	MS.gene061959:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAAGATTGATTTTGTTGTTG+AGG	-	chr6.2:20951263-20951282	None:intergenic	25.0%
	AACATATTCCAAATGCAAGA+AGG	+	chr6.2:20951624-20951643	MS.gene061959:CDS	30.0%
	CATTCACATCTTATAAGTGA+TGG	+	chr6.2:20951362-20951381	MS.gene061959:CDS	30.0%
	CTTTGTTGAAACAAGCTTAT+TGG	+	chr6.2:20951405-20951424	MS.gene061959:CDS	30.0%
!	ATGTGAATGTGACCAAATTT+TGG	-	chr6.2:20951351-20951370	None:intergenic	30.0%
!	CAATAAGCTTGTTTCAACAA+AGG	-	chr6.2:20951407-20951426	None:intergenic	30.0%
!	TGATGTTAATGATGATGATG+AGG	+	chr6.2:20951601-20951620	MS.gene061959:CDS	30.0%
!!	GAGACTTTTTGCTCATTTTT+CGG	-	chr6.2:20951455-20951474	None:intergenic	30.0%
!!!	TTGCAAAATTTTGTTGTTCC+TGG	+	chr6.2:20951284-20951303	MS.gene061959:CDS	30.0%
	ATCAGACAGAAGCAAAAAGA+AGG	+	chr6.2:20951492-20951511	MS.gene061959:CDS	35.0%
	TCAGACAGAAGCAAAAAGAA+GGG	+	chr6.2:20951493-20951512	MS.gene061959:CDS	35.0%
	TGCAAGTAGCTAACAAAAGT+AGG	-	chr6.2:20951782-20951801	None:intergenic	35.0%
!	AAGCTTGTTTCAACAAAGGA+GGG	-	chr6.2:20951403-20951422	None:intergenic	35.0%
!	GAAAGAGAAAGAGAGTTTTG+AGG	+	chr6.2:20951538-20951557	MS.gene061959:CDS	35.0%
!	TAAGCTTGTTTCAACAAAGG+AGG	-	chr6.2:20951404-20951423	None:intergenic	35.0%
!!	TGTGACCAAATTTTGGTACT+AGG	-	chr6.2:20951344-20951363	None:intergenic	35.0%
	AAAAAGAAGGGTGAAGAGAG+TGG	+	chr6.2:20951505-20951524	MS.gene061959:CDS	40.0%
	AAGACACTATGCAATGCATG+TGG	+	chr6.2:20951704-20951723	MS.gene061959:CDS	40.0%
	CAGCTCCTAGTACCAAAATT+TGG	+	chr6.2:20951336-20951355	MS.gene061959:CDS	40.0%
	CATGCATTGCATAGTGTCTT+TGG	-	chr6.2:20951704-20951723	None:intergenic	40.0%
	GCACTCATTGTTTGTCACAA+AGG	+	chr6.2:20951648-20951667	MS.gene061959:CDS	40.0%
	GGTGAAGAGAGTGGTAAAAA+TGG	+	chr6.2:20951514-20951533	MS.gene061959:CDS	40.0%
!!!	GAGAAAGAGAGTTTTGAGGT+TGG	+	chr6.2:20951542-20951561	MS.gene061959:CDS	40.0%
	ACAAAAGTAGGACTCTTCGC+TGG	-	chr6.2:20951770-20951789	None:intergenic	45.0%
	CATAGTGTCTTTGGTCCCAA+TGG	-	chr6.2:20951695-20951714	None:intergenic	45.0%
	GTAGGTTGTTGCCAGAGTAT+AGG	+	chr6.2:20951744-20951763	MS.gene061959:CDS	45.0%
	TATGCAATGCATGTGGTGTG+AGG	+	chr6.2:20951711-20951730	MS.gene061959:CDS	45.0%
	TGAGTGCACCTTCTTGCATT+TGG	-	chr6.2:20951635-20951654	None:intergenic	45.0%
!!	CTCTTGCTTCTTGCTTTTCC+AGG	-	chr6.2:20951305-20951324	None:intergenic	45.0%
	AAAGTCTCTGCAGTTGCGTG+CGG	+	chr6.2:20951467-20951486	MS.gene061959:CDS	50.0%
	ACAAAGGACCCCACAATGGA+GGG	+	chr6.2:20951664-20951683	MS.gene061959:CDS	50.0%
	GTGTGAGGTACAAGTCTGGT+AGG	+	chr6.2:20951726-20951745	MS.gene061959:CDS	50.0%
	TGTCACAAAGGACCCCACAA+TGG	+	chr6.2:20951660-20951679	MS.gene061959:CDS	50.0%
	TGTGGTGTGAGGTACAAGTC+TGG	+	chr6.2:20951722-20951741	MS.gene061959:CDS	50.0%
	AATGGTCCTGCCCTCCATTG+TGG	-	chr6.2:20951677-20951696	None:intergenic	55.0%
	ACAATGGAGGGCAGGACCAT+TGG	+	chr6.2:20951676-20951695	MS.gene061959:CDS	55.0%
	ATGGTCCTGCCCTCCATTGT+GGG	-	chr6.2:20951676-20951695	None:intergenic	55.0%
	CAATGGAGGGCAGGACCATT+GGG	+	chr6.2:20951677-20951696	MS.gene061959:CDS	55.0%
	CACAAAGGACCCCACAATGG+AGG	+	chr6.2:20951663-20951682	MS.gene061959:CDS	55.0%
	CGGAGCAATGAGTTCGCTGT+CGG	-	chr6.2:20951435-20951454	None:intergenic	55.0%
!	CTCTTCGCTGGCCTATACTC+TGG	-	chr6.2:20951758-20951777	None:intergenic	55.0%
	TGGTCCTGCCCTCCATTGTG+GGG	-	chr6.2:20951675-20951694	None:intergenic	60.0%
	AGGACCCCACAATGGAGGGC+AGG	+	chr6.2:20951668-20951687	MS.gene061959:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	20951251	20951853	20951251	ID=MS.gene061959
chr6.2	mRNA	20951251	20951853	20951251	ID=MS.gene061959.t1;Parent=MS.gene061959
chr6.2	exon	20951251	20951853	20951251	ID=MS.gene061959.t1.exon1;Parent=MS.gene061959.t1
chr6.2	CDS	20951251	20951853	20951251	ID=cds.MS.gene061959.t1;Parent=MS.gene061959.t1

Gene Sequence

>MS.gene061959

ATGCAAAAACCTCAACAACAAAATCAATCTTATTTGCAAAATTTTGTTGTTCCTGGAAAAGCAAGAAGCAAGAGAAAAAGACTTTCAGCTCCTAGTACCAAAATTTGGTCACATTCACATCTTATAAGTGATGGTAATTTGATTTCTGACCCTCCTTTGTTGAAACAAGCTTATTGGTTAGCCGACAGCGAACTCATTGCTCCGAAAAATGAGCAAAAAGTCTCTGCAGTTGCGTGCGGAGATCAGACAGAAGCAAAAAGAAGGGTGAAGAGAGTGGTAAAAATGGAGAAAGAGAAAGAGAGTTTTGAGGTTGGAATTACTCAAGTTAAGCATAGTGAGAATAATAATGATGATGTTAATGATGATGATGAGGAACATATTCCAAATGCAAGAAGGTGCACTCATTGTTTGTCACAAAGGACCCCACAATGGAGGGCAGGACCATTGGGACCAAAGACACTATGCAATGCATGTGGTGTGAGGTACAAGTCTGGTAGGTTGTTGCCAGAGTATAGGCCAGCGAAGAGTCCTACTTTTGTTAGCTACTTGCATTCAAATTCACACAAGAAAGTTCTTGAGATGAGAATGCAATCTATTAAGTAG

Protein sequence

>MS.gene061959.t1

MQKPQQQNQSYLQNFVVPGKARSKRKRLSAPSTKIWSHSHLISDGNLISDPPLLKQAYWLADSELIAPKNEQKVSAVACGDQTEAKRRVKRVVKMEKEKESFEVGITQVKHSENNNDDVNDDDEEHIPNARRCTHCLSQRTPQWRAGPLGPKTLCNACGVRYKSGRLLPEYRPAKSPTFVSYLHSNSHKKVLEMRMQSIK