AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06074


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06074.t1 MTR_8g099495 99.048 105 1 0 1 105 175 279 1.99e-73 225
MS.gene06074.t1 MTR_8g099515 43.011 93 48 3 17 104 174 266 2.62e-16 74.7
MS.gene06074.t1 MTR_3g091200 31.897 116 65 4 3 104 145 260 7.97e-16 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06074.t1 AT5G53110 29.412 102 61 2 14 104 157 258 3.74e-11 59.7

Find 42 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGTCCTCAATACTTCATT+TGG 0.314741 4.1:+4582670 None:intergenic
AAGAGACGAATTGGTTGTTT+TGG 0.346013 4.1:-4583983 MS.gene06074:CDS
GAGGAAGATGTGGATTGGTT+GGG 0.360207 4.1:-4583893 MS.gene06074:CDS
ATCTTCCACTTTCACCAAGC+TGG 0.369581 4.1:+4582743 None:intergenic
CTTTCAAGGAAAGTCAAGTA+TGG 0.374180 4.1:-4582958 MS.gene06074:CDS
GTATGGTTTAAGCCTAGGTT+TGG 0.409546 4.1:-4582941 MS.gene06074:CDS
TATGGTTTAAGCCTAGGTTT+GGG 0.424366 4.1:-4582940 MS.gene06074:CDS
GCTTCACAATTTCCACAATC+AGG 0.434686 4.1:+4583921 None:intergenic
TCTTCCACTTTCACCAAGCT+GGG 0.464098 4.1:+4582744 None:intergenic
AGCCTAGGTTTGGGAATACC+TGG 0.477958 4.1:-4582931 MS.gene06074:CDS
GGAGGAAGATGTGGATTGGT+TGG 0.478263 4.1:-4583894 MS.gene06074:CDS
TACCGGTGGAAGAGACGAAT+TGG 0.478685 4.1:-4583992 MS.gene06074:CDS
GTCAAGTATGGTTTAAGCCT+AGG 0.480880 4.1:-4582946 MS.gene06074:CDS
GTCTGCATGTAGGTCTTTCA+AGG 0.481860 4.1:-4582972 MS.gene06074:intron
AGGAAGATGTGGATTGGTTG+GGG 0.491695 4.1:-4583892 MS.gene06074:CDS
AAAACCCAGCTTGGTGAAAG+TGG 0.493961 4.1:-4582748 MS.gene06074:CDS
TTGGGAATACCTGGACTCCT+AGG 0.494340 4.1:-4582922 MS.gene06074:intron
ATTGACTATTTCACCTTGGG+TGG 0.497251 4.1:+4583836 None:intergenic
CAAATGTTGTCACTTGGCCT+AGG 0.507133 4.1:+4582718 None:intergenic
GATGTCCACGGTGTAGCAAA+AGG 0.511204 4.1:+4584044 None:intergenic
GAACATGAATTATTCGATTG+TGG 0.526227 4.1:-4584084 None:intergenic
TATTTCGTCAGCTTTGATAC+CGG 0.536087 4.1:-4584009 MS.gene06074:CDS
AACCAATTCGTCTCTTCCAC+CGG 0.549752 4.1:+4583990 None:intergenic
ACAATTAGACCTAGGAGTCC+AGG 0.561924 4.1:+4582913 None:intergenic
TTCAAACATACCGTTGCAGT+GGG 0.575801 4.1:-4583949 MS.gene06074:CDS
TGTTATGATCTTCCCACCCA+AGG 0.578358 4.1:-4583849 MS.gene06074:intron
ATCAGCCAAATGAAGTATTG+AGG 0.579109 4.1:-4582675 None:intergenic
AGATACCCTAAAACCCAGCT+TGG 0.586664 4.1:-4582757 MS.gene06074:CDS
ATTCAAACATACCGTTGCAG+TGG 0.592135 4.1:-4583950 MS.gene06074:CDS
CAGTGGGATGATCCTGATTG+TGG 0.594321 4.1:-4583933 MS.gene06074:CDS
TTCGTCAGCTTTGATACCGG+TGG 0.597171 4.1:-4584006 MS.gene06074:CDS
TTGACTATTTCACCTTGGGT+GGG 0.599087 4.1:+4583837 None:intergenic
GTGAAAGTGGAAGATTGCCT+AGG 0.611829 4.1:-4582735 MS.gene06074:CDS
ATAGAGCAAATGTTGTCACT+TGG 0.619697 4.1:+4582712 None:intergenic
AATTCACATAATGATGTCCA+CGG 0.636628 4.1:+4584032 None:intergenic
TCAGGATCATCCCACTGCAA+CGG 0.649351 4.1:+4583939 None:intergenic
GAAGCACGTGGAGGAAGATG+TGG 0.650882 4.1:-4583903 MS.gene06074:CDS
ACGTGGAGGAAGATGTGGAT+TGG 0.656368 4.1:-4583898 MS.gene06074:CDS
GTCCACGGTGTAGCAAAAGG+AGG 0.695275 4.1:+4584047 None:intergenic
GTCCAGGTATTCCCAAACCT+AGG 0.704709 4.1:+4582929 None:intergenic
TGTGGAAATTGTGAAGCACG+TGG 0.742242 4.1:-4583915 MS.gene06074:CDS
GGAAATTGTGAAGCACGTGG+AGG 0.769055 4.1:-4583912 MS.gene06074:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATATCAAAATAGATA+CGG - chr4.1:4583074-4583093 MS.gene06074:intron 10.0%
!! AAAAAACATGAAGTTTAATT+TGG - chr4.1:4583181-4583200 MS.gene06074:intron 15.0%
!! AACAAGTTATGTAATTAATT+AGG + chr4.1:4583733-4583752 None:intergenic 15.0%
!!! TTTTGATATTTTTTTTTCCA+AGG + chr4.1:4583068-4583087 None:intergenic 15.0%
!! AAAATCAAATCAAACAAATG+CGG - chr4.1:4583223-4583242 MS.gene06074:intron 20.0%
!! ACATTTGATTCAAATGTATT+TGG + chr4.1:4583366-4583385 None:intergenic 20.0%
!!! TCATGTTTTTTTTTATCTTG+CGG + chr4.1:4583172-4583191 None:intergenic 20.0%
!!! TGCAAATTTAGATCATATTT+TGG - chr4.1:4582968-4582987 MS.gene06074:intron 20.0%
!!! TTTGGTTTTCTTTTAACTTT+TGG - chr4.1:4582986-4583005 MS.gene06074:intron 20.0%
! TGTAATTAATTAGGATCCTT+AGG + chr4.1:4583724-4583743 None:intergenic 25.0%
!!! AATAGTTGTGTTGTTTTCAT+TGG + chr4.1:4583462-4583481 None:intergenic 25.0%
!!! AGTTTCAATTTGGATTAGTT+CGG - chr4.1:4583395-4583414 MS.gene06074:intron 25.0%
!!! ATAGTTGTGTTGTTTTCATT+GGG + chr4.1:4583461-4583480 None:intergenic 25.0%
!!! ATTTTTTTGTGTGTTTAGCT+CGG - chr4.1:4583279-4583298 MS.gene06074:intron 25.0%
!!! TTTGGTTTTGTTTGATTTTG+AGG - chr4.1:4583129-4583148 MS.gene06074:intron 25.0%
!!! TTTTTTTTCCAAGGACTATT+TGG + chr4.1:4583059-4583078 None:intergenic 25.0%
AACTAAACGATTGTGTTTCA+TGG + chr4.1:4583509-4583528 None:intergenic 30.0%
AAGACCCATAACAAAAATTG+GGG + chr4.1:4583962-4583981 None:intergenic 30.0%
AATTCACATAATGATGTCCA+CGG + chr4.1:4582728-4582747 None:intergenic 30.0%
AGAAATATCCAAATAGTCCT+TGG - chr4.1:4583048-4583067 MS.gene06074:intron 30.0%
ATGTAAACACAATTAGACCT+AGG + chr4.1:4583855-4583874 None:intergenic 30.0%
CAAGACCCATAACAAAAATT+GGG + chr4.1:4583963-4583982 None:intergenic 30.0%
TAGGTCTAATTGTGTTTACA+TGG - chr4.1:4583854-4583873 MS.gene06074:CDS 30.0%
TATGCAAAAAGAAGAACACA+AGG - chr4.1:4583881-4583900 MS.gene06074:CDS 30.0%
TCAAGACCCATAACAAAAAT+TGG + chr4.1:4583964-4583983 None:intergenic 30.0%
TGAAACACAATCGTTTAGTT+TGG - chr4.1:4583509-4583528 MS.gene06074:intron 30.0%
TTGAAAATTCAGTGCTTGTT+TGG + chr4.1:4583921-4583940 None:intergenic 30.0%
! ATCATTGACTATTTCACCTT+GGG + chr4.1:4582927-4582946 None:intergenic 30.0%
! GAAACACAATCGTTTAGTTT+GGG - chr4.1:4583510-4583529 MS.gene06074:intron 30.0%
! TTCTTTTCCGTTGTTCTTTT+GGG - chr4.1:4583762-4583781 MS.gene06074:intron 30.0%
! TTTCTTTTCCGTTGTTCTTT+TGG - chr4.1:4583761-4583780 MS.gene06074:intron 30.0%
!! TCGTTTTTGCAGTTTCAATT+TGG - chr4.1:4583385-4583404 MS.gene06074:intron 30.0%
!!! ATTCATAGTGCATTTTGGTT+TGG - chr4.1:4583111-4583130 MS.gene06074:intron 30.0%
!!! GTTTTTCTATTGTGTTGGTT+TGG - chr4.1:4583424-4583443 MS.gene06074:intron 30.0%
!!! TAGTTGTGTTGTTTTCATTG+GGG + chr4.1:4583460-4583479 None:intergenic 30.0%
AATCTCCACTATAAGTCCTA+AGG - chr4.1:4583705-4583724 MS.gene06074:intron 35.0%
AGACCCATAACAAAAATTGG+GGG + chr4.1:4583961-4583980 None:intergenic 35.0%
ATAGAGCAAATGTTGTCACT+TGG + chr4.1:4584048-4584067 None:intergenic 35.0%
CAAATCAAACAAATGCGGTT+TGG - chr4.1:4583228-4583247 MS.gene06074:intron 35.0%
CTTTCAAGGAAAGTCAAGTA+TGG - chr4.1:4583799-4583818 MS.gene06074:intron 35.0%
TATGGTTTAAGCCTAGGTTT+GGG - chr4.1:4583817-4583836 MS.gene06074:intron 35.0%
! AAGAGACGAATTGGTTGTTT+TGG - chr4.1:4582774-4582793 MS.gene06074:intron 35.0%
! CATCATTGACTATTTCACCT+TGG + chr4.1:4582928-4582947 None:intergenic 35.0%
! TATTTCGTCAGCTTTGATAC+CGG - chr4.1:4582748-4582767 MS.gene06074:CDS 35.0%
! TCTTTTCCGTTGTTCTTTTG+GGG - chr4.1:4583763-4583782 MS.gene06074:intron 35.0%
!! GAGGTATTCATAGTGCATTT+TGG - chr4.1:4583106-4583125 MS.gene06074:intron 35.0%
!! TTTTGTTATGGGTCTTGACA+GGG - chr4.1:4583966-4583985 MS.gene06074:CDS 35.0%
!! TTTTTGTTATGGGTCTTGAC+AGG - chr4.1:4583965-4583984 MS.gene06074:CDS 35.0%
!!! AGTTGTGTTGTTTTCATTGG+GGG + chr4.1:4583459-4583478 None:intergenic 35.0%
AGGATCCTTAGGACTTATAG+TGG + chr4.1:4583713-4583732 None:intergenic 40.0%
ATTCAAACATACCGTTGCAG+TGG - chr4.1:4582807-4582826 MS.gene06074:intron 40.0%
ATTGACTATTTCACCTTGGG+TGG + chr4.1:4582924-4582943 None:intergenic 40.0%
GACCCATAACAAAAATTGGG+GGG + chr4.1:4583960-4583979 None:intergenic 40.0%
GCAAATTGAACTGAACCATG+CGG - chr4.1:4583320-4583339 MS.gene06074:intron 40.0%
GCATGGTTCAGTTCAATTTG+CGG + chr4.1:4583321-4583340 None:intergenic 40.0%
GCTTCACAATTTCCACAATC+AGG + chr4.1:4582839-4582858 None:intergenic 40.0%
GTATGGTTTAAGCCTAGGTT+TGG - chr4.1:4583816-4583835 MS.gene06074:intron 40.0%
GTCAAGTATGGTTTAAGCCT+AGG - chr4.1:4583811-4583830 MS.gene06074:intron 40.0%
GTCTACATTTGCAACTACAG+CGG - chr4.1:4583532-4583551 MS.gene06074:intron 40.0%
TTCAAACATACCGTTGCAGT+GGG - chr4.1:4582808-4582827 MS.gene06074:intron 40.0%
TTGACTATTTCACCTTGGGT+GGG + chr4.1:4582923-4582942 None:intergenic 40.0%
! TCGCGGTTTTTCTATTGTGT+TGG - chr4.1:4583419-4583438 MS.gene06074:intron 40.0%
! TTTCACCAAGCTGGGTTTTA+GGG + chr4.1:4584008-4584027 None:intergenic 40.0%
!! ATTTGGATTAGTTCGGTTCG+CGG - chr4.1:4583402-4583421 MS.gene06074:intron 40.0%
!! CAAACAAATGCGGTTTGGTT+TGG - chr4.1:4583233-4583252 MS.gene06074:intron 40.0%
!! CACCCCCCAATTTTTGTTAT+GGG - chr4.1:4583955-4583974 MS.gene06074:CDS 40.0%
!!! CTTTTTTTTACTCAGCCGCA+TGG + chr4.1:4583338-4583357 None:intergenic 40.0%
AACCAATTCGTCTCTTCCAC+CGG + chr4.1:4582770-4582789 None:intergenic 45.0%
ACAATTAGACCTAGGAGTCC+AGG + chr4.1:4583847-4583866 None:intergenic 45.0%
AGATACCCTAAAACCCAGCT+TGG - chr4.1:4584000-4584019 MS.gene06074:CDS 45.0%
ATAGATACGGCGAGAACTAG+AGG - chr4.1:4583087-4583106 MS.gene06074:intron 45.0%
ATCTTCCACTTTCACCAAGC+TGG + chr4.1:4584017-4584036 None:intergenic 45.0%
GTCTGCATGTAGGTCTTTCA+AGG - chr4.1:4583785-4583804 MS.gene06074:intron 45.0%
GTGAAAGTGGAAGATTGCCT+AGG - chr4.1:4584022-4584041 MS.gene06074:CDS 45.0%
TCTTCCACTTTCACCAAGCT+GGG + chr4.1:4584016-4584035 None:intergenic 45.0%
TGCAGACCCCAAAAGAACAA+CGG + chr4.1:4583772-4583791 None:intergenic 45.0%
TGTGGAAATTGTGAAGCACG+TGG - chr4.1:4582842-4582861 MS.gene06074:intron 45.0%
TGTTATGATCTTCCCACCCA+AGG - chr4.1:4582908-4582927 MS.gene06074:intron 45.0%
! AAAACCCAGCTTGGTGAAAG+TGG - chr4.1:4584009-4584028 MS.gene06074:CDS 45.0%
! CAAATGTTGTCACTTGGCCT+AGG + chr4.1:4584042-4584061 None:intergenic 45.0%
! CTTTCACCAAGCTGGGTTTT+AGG + chr4.1:4584009-4584028 None:intergenic 45.0%
! GCACCCCCCAATTTTTGTTA+TGG - chr4.1:4583954-4583973 MS.gene06074:CDS 45.0%
!! AGGAAGATGTGGATTGGTTG+GGG - chr4.1:4582865-4582884 MS.gene06074:intron 45.0%
!! GAGGAAGATGTGGATTGGTT+GGG - chr4.1:4582864-4582883 MS.gene06074:intron 45.0%
!! GCGGTTTGGTTTGGTTGTTA+CGG - chr4.1:4583242-4583261 MS.gene06074:intron 45.0%
!!! TTCTTTTGGGGTCTGCATGT+AGG - chr4.1:4583775-4583794 MS.gene06074:intron 45.0%
ACGTGGAGGAAGATGTGGAT+TGG - chr4.1:4582859-4582878 MS.gene06074:intron 50.0%
AGCCTAGGTTTGGGAATACC+TGG - chr4.1:4583826-4583845 MS.gene06074:intron 50.0%
CAGTGGGATGATCCTGATTG+TGG - chr4.1:4582824-4582843 MS.gene06074:intron 50.0%
GATGTCCACGGTGTAGCAAA+AGG + chr4.1:4582716-4582735 None:intergenic 50.0%
GGAAATTGTGAAGCACGTGG+AGG - chr4.1:4582845-4582864 MS.gene06074:intron 50.0%
GTCCAGGTATTCCCAAACCT+AGG + chr4.1:4583831-4583850 None:intergenic 50.0%
TACCGGTGGAAGAGACGAAT+TGG - chr4.1:4582765-4582784 MS.gene06074:CDS 50.0%
TCAGGATCATCCCACTGCAA+CGG + chr4.1:4582821-4582840 None:intergenic 50.0%
TTGGGAATACCTGGACTCCT+AGG - chr4.1:4583835-4583854 MS.gene06074:intron 50.0%
! ATCCTCCTTTTGCTACACCG+TGG - chr4.1:4582708-4582727 MS.gene06074:CDS 50.0%
! TGGGGGGTGCGTCATAATAA+AGG + chr4.1:4583944-4583963 None:intergenic 50.0%
! TTCGTCAGCTTTGATACCGG+TGG - chr4.1:4582751-4582770 MS.gene06074:CDS 50.0%
!! GGAGGAAGATGTGGATTGGT+TGG - chr4.1:4582863-4582882 MS.gene06074:intron 50.0%
GAAGCACGTGGAGGAAGATG+TGG - chr4.1:4582854-4582873 MS.gene06074:intron 55.0%
GTCCACGGTGTAGCAAAAGG+AGG + chr4.1:4582713-4582732 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 4582677 4584102 4582677 ID=MS.gene06074
chr4.1 mRNA 4582677 4584102 4582677 ID=MS.gene06074.t1;Parent=MS.gene06074
chr4.1 exon 4583850 4584102 4583850 ID=MS.gene06074.t1.exon1;Parent=MS.gene06074.t1
chr4.1 CDS 4583850 4584102 4583850 ID=cds.MS.gene06074.t1;Parent=MS.gene06074.t1
chr4.1 exon 4582923 4582982 4582923 ID=MS.gene06074.t1.exon2;Parent=MS.gene06074.t1
chr4.1 CDS 4582923 4582982 4582923 ID=cds.MS.gene06074.t1;Parent=MS.gene06074.t1
chr4.1 exon 4582677 4582792 4582677 ID=MS.gene06074.t1.exon3;Parent=MS.gene06074.t1
chr4.1 CDS 4582677 4582792 4582677 ID=cds.MS.gene06074.t1;Parent=MS.gene06074.t1
Gene Sequence

>MS.gene06074

ATGAATTATTCGATTGTGGCTATGATGTCTGATCCTCCTTTTGCTACACCGTGGACATCATTATGTGAATTTATTTCGTCAGCTTTGATACCGGTGGAAGAGACGAATTGGTTGTTTTGGACTGATTATTATTCAAACATACCGTTGCAGTGGGATGATCCTGATTGTGGAAATTGTGAAGCACGTGGAGGAAGATGTGGATTGGTTGGGGATGATGCTCTTCGTCTTGCTTGTTATGATCTTCCCACCCAAGGTGAAATAGTCAATGATGTTTTATTTTCACTCATTTCTTGCAAATTTAGATCATATTTTGGTTTTCTTTTAACTTTTGGCGAATTCTTATTTTTAGACTTTTTTTTTTCTTACTTATTAGAAATATCCAAATAGTCCTTGGAAAAAAAAATATCAAAATAGATACGGCGAGAACTAGAGGTATTCATAGTGCATTTTGGTTTGGTTTTGTTTGATTTTGAGGTTAAAAAGTCATATTAACCGCAAGATAAAAAAAAACATGAAGTTTAATTTGGTCAGTTGACTTTTAAAATGAAAATCAAATCAAACAAATGCGGTTTGGTTTGGTTGTTACGGTTTTTTGCGACACAATTTTTTTGTGTGTTTAGCTCGGTTGCTAAAATAAAAAACCGCAAATTGAACTGAACCATGCGGCTGAGTAAAAAAAAGAAGATCCAAATACATTTGAATCAAATGTCGTTTTTGCAGTTTCAATTTGGATTAGTTCGGTTCGCGGTTTTTCTATTGTGTTGGTTTGGTTTTGAACACCCCCAATGAAAACAACACAACTATTCATAGAAGACTGAAGAAAACACAACCATGAAACACAATCGTTTAGTTTGGGTCTACATTTGCAACTACAGCGGATTTTTTTTTTGAAAATTTTTGTACAAAAAATTATCTTGTTTAAATGTTTATGAGAAAATAAAATAAAAATTATTGATTAAAAGATATTATTTTTTGCTCTAATTAACTCAACTTTTTTTCTTACACAAGAAAATTAACAACTGACTTAGAATCTCCACTATAAGTCCTAAGGATCCTAATTAATTACATAACTTGTTTTTTTCTTTTTCTTTTCCGTTGTTCTTTTGGGGTCTGCATGTAGGTCTTTCAAGGAAAGTCAAGTATGGTTTAAGCCTAGGTTTGGGAATACCTGGACTCCTAGGTCTAATTGTGTTTACATGGATGTTATGCAAAAAGAAGAACACAAGGAATCAAGTACAACGCCAAACAAGCACTGAATTTTCAACCTTTATTATGACGCACCCCCCAATTTTTGTTATGGGTCTTGACAGGGCAACAATCGAAAGATACCCTAAAACCCAGCTTGGTGAAAGTGGAAGATTGCCTAGGCCAAGTGACAACATTTGCTCTATATGTCTTTGTGAGTATCAGCCAAATGAAGTATTGA

Protein sequence

>MS.gene06074.t1

MNYSIVAMMSDPPFATPWTSLCEFISSALIPVEETNWLFWTDYYSNIPLQWDDPDCGNCEARGGRCGLVGDDALRLACYDLPTQGLSRKVKYGLSLGLGIPGLLGQQSKDTLKPSLVKVEDCLGQVTTFALYVFVSISQMKY