AlfalfaGEDB | MS.gene062141

M. sativa cultivar XinJiangDaYe / MS.gene062141

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062141.t1	XP_013463600.1	90.2	214	17	2	1	214	1	210	1.20E-95	359.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062141.t1	A0A072VHK0	90.2	214	17	2	1	214	1	210	8.5e-96	359.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056236	MS.gene062141	0.820615	6.25E-53	-1.69E-46
MS.gene058459	MS.gene062141	0.802635	5.11E-49	-1.69E-46
MS.gene059711	MS.gene062141	0.803198	3.91E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062141.t1	MTR_2g043710	89.720	214	18	2	1	214	1	210	3.49e-136	380
MS.gene062141.t1	MTR_4g124850	39.048	210	81	11	1	196	1	177	2.45e-30	110

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062141.t1	AT5G20790	36.139	202	111	8	5	200	4	193	3.31e-25	97.8
MS.gene062141.t1	AT3G43110	36.269	193	94	9	5	191	4	173	7.70e-25	96.7
MS.gene062141.t1	AT5G20790	37.097	124	68	4	5	125	4	120	4.38e-13	64.3

Find 59 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGGAAACGTGGTTAAATTT+TGG	0.094890	2.3:+46415900	MS.gene062141:CDS
TCACAATTCTCAACAACTTA+TGG	0.210366	2.3:+46415420	MS.gene062141:CDS
GGGCAATTGCCGGTTGTTAA+AGG	0.224855	2.3:+46415764	MS.gene062141:CDS
TCACCGCCGCTCTCAGCTTC+TGG	0.279525	2.3:+46415456	MS.gene062141:CDS
GATCAAGGTGTCTCAGTTTC+CGG	0.291224	2.3:+46415533	MS.gene062141:CDS
CAAGATTTAACGGAGCTTAA+TGG	0.294575	2.3:+46415881	MS.gene062141:CDS
CACCACTCACCTTCAACTTC+CGG	0.299818	2.3:-46415794	None:intergenic
TGAAACCGGGTCTAGTGATT+TGG	0.305691	2.3:-46415501	None:intergenic
GCCTTCAACTACTTAAACTT+TGG	0.352929	2.3:+46415392	MS.gene062141:CDS
GGCAATTGCCGGTTGTTAAA+GGG	0.397479	2.3:+46415765	MS.gene062141:CDS
ACCAAAGTTTAAGTAGTTGA+AGG	0.401290	2.3:-46415393	None:intergenic
GTCTAGTGATTTGGGACAAC+CGG	0.412402	2.3:-46415492	None:intergenic
TTCTGGAAGATTCGCTCATC+CGG	0.418852	2.3:+46415473	MS.gene062141:CDS
GACGGTGGGTTAACGTTTGT+TGG	0.419622	2.3:+46415923	MS.gene062141:CDS
TAATTGTTACGGGCAATTGC+CGG	0.423106	2.3:+46415754	MS.gene062141:CDS
AAGGCATTAAGGTTGAGAAA+TGG	0.428704	2.3:+46415830	MS.gene062141:CDS
GAAACCGGGTCTAGTGATTT+GGG	0.430187	2.3:-46415500	None:intergenic
GGTTCCCACCCTTTAACAAC+CGG	0.439245	2.3:-46415773	None:intergenic
AAGGTGAGTGGTGGAAATGT+TGG	0.461562	2.3:+46415804	MS.gene062141:CDS
TTCTCAACAACTTATGGACT+TGG	0.465068	2.3:+46415426	MS.gene062141:CDS
AAAAGACATACATCTGGAAC+TGG	0.465501	2.3:-46415972	None:intergenic
AAGAGTAGTAGTAATTGTTA+CGG	0.475526	2.3:+46415743	MS.gene062141:CDS
AGAGTAGTAGTAATTGTTAC+GGG	0.476807	2.3:+46415744	MS.gene062141:CDS
ATCCACATTGCATGTCCTCT+TGG	0.480252	2.3:-46415715	None:intergenic
TTCCGGTGTTACATCTCCGC+TGG	0.486135	2.3:+46415550	MS.gene062141:CDS
AATGTTGGGAGAAGGCATTA+AGG	0.487030	2.3:+46415819	MS.gene062141:CDS
TCATTCAAAAGACATACATC+TGG	0.498502	2.3:-46415978	None:intergenic
AGAAATGGAGAGAATGAGAA+TGG	0.506297	2.3:+46415845	MS.gene062141:CDS
AGACCCGGTTTCAGTAAAAC+CGG	0.515205	2.3:+46415511	MS.gene062141:CDS
ATGTTGACGGTGTGAGGAAG+GGG	0.516175	2.3:+46415675	MS.gene062141:CDS
GGTTGTTAAAGGGTGGGAAC+CGG	0.517768	2.3:+46415775	MS.gene062141:CDS
GAAACTGAGACACCTTGATC+CGG	0.523489	2.3:-46415530	None:intergenic
TGATGTTGACGGTGTGAGGA+AGG	0.525611	2.3:+46415673	MS.gene062141:CDS
AGGTGAGTGGTGGAAATGTT+GGG	0.538056	2.3:+46415805	MS.gene062141:CDS
GGGTGGGAACCGGAAGTTGA+AGG	0.538451	2.3:+46415785	MS.gene062141:CDS
GTTGCATCACAAATGAATCA+TGG	0.548908	2.3:+46415948	MS.gene062141:CDS
GTACACGTGTCAAGATTTAA+CGG	0.562622	2.3:+46415871	MS.gene062141:CDS
GATGTTGACGGTGTGAGGAA+GGG	0.574143	2.3:+46415674	MS.gene062141:CDS
ATGGAGAGAATGAGAATGGG+TGG	0.576348	2.3:+46415849	MS.gene062141:CDS
CGGTGGAAATTAAGACACTT+TGG	0.577700	2.3:-46415586	None:intergenic
GTTTCAGTAAAACCGGATCA+AGG	0.580100	2.3:+46415518	MS.gene062141:CDS
TTGTCCCAAATCACTAGACC+CGG	0.581843	2.3:+46415496	MS.gene062141:CDS
AATTGCCGGTTGTTAAAGGG+TGG	0.596569	2.3:+46415768	MS.gene062141:CDS
GAAATGGAGAGAATGAGAAT+GGG	0.611130	2.3:+46415846	MS.gene062141:CDS
TTGATGATGTTGACGGTGTG+AGG	0.619171	2.3:+46415669	MS.gene062141:CDS
ATTGCCGGTTGTTAAAGGGT+GGG	0.620883	2.3:+46415769	MS.gene062141:CDS
GTGGTGGAAATGTTGGGAGA+AGG	0.622094	2.3:+46415811	MS.gene062141:CDS
GAAATTAAGACACTTTGGTG+TGG	0.625936	2.3:-46415581	None:intergenic
CGGAAGTTGAAGGTGAGTGG+TGG	0.634071	2.3:+46415795	MS.gene062141:CDS
CTTGTTAGATTCCAACGTTG+AGG	0.649147	2.3:+46415364	MS.gene062141:CDS
AACCGGAAGTTGAAGGTGAG+TGG	0.654999	2.3:+46415792	MS.gene062141:CDS
CTTCCAGAAGCTGAGAGCGG+CGG	0.657609	2.3:-46415459	None:intergenic
AACGGAGCTTAATGGAAACG+TGG	0.662549	2.3:+46415889	MS.gene062141:CDS
TTCCAGCGGAGATGTAACAC+CGG	0.667046	2.3:-46415552	None:intergenic
CAACGTTGGAATCTAACAAG+AGG	0.669129	2.3:-46415361	None:intergenic
TACCAAGAGGACATGCAATG+TGG	0.670875	2.3:+46415713	MS.gene062141:CDS
GTTTACGTTGTATTACCAAG+AGG	0.673361	2.3:+46415700	MS.gene062141:CDS
GAAGGCTAAAGCCTCAACGT+TGG	0.676990	2.3:-46415375	None:intergenic
AATCTTCCAGAAGCTGAGAG+CGG	0.687123	2.3:-46415462	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGAGTAGTAGTAATTGTTA+CGG	+	chr2.3:46415743-46415762	MS.gene062141:CDS	25.0%
!!!	AATGATTTTGATGATGTTGA+CGG	+	chr2.3:46415662-46415681	MS.gene062141:CDS	25.0%
	ACCAAAGTTTAAGTAGTTGA+AGG	-	chr2.3:46415396-46415415	None:intergenic	30.0%
	AGAGTAGTAGTAATTGTTAC+GGG	+	chr2.3:46415744-46415763	MS.gene062141:CDS	30.0%
	TCACAATTCTCAACAACTTA+TGG	+	chr2.3:46415420-46415439	MS.gene062141:CDS	30.0%
	TCATTCAAAAGACATACATC+TGG	-	chr2.3:46415981-46416000	None:intergenic	30.0%
!	ATGGAAACGTGGTTAAATTT+TGG	+	chr2.3:46415900-46415919	MS.gene062141:CDS	30.0%
!	CGTTACATTTTCATCTGTAA+CGG	-	chr2.3:46415609-46415628	None:intergenic	30.0%
!	TGGAAACGTGGTTAAATTTT+GGG	+	chr2.3:46415901-46415920	MS.gene062141:CDS	30.0%
!!!	TCATTTGATGATGTTTTACG+AGG	-	chr2.3:46415647-46415666	None:intergenic	30.0%
	AGAAATGGAGAGAATGAGAA+TGG	+	chr2.3:46415845-46415864	MS.gene062141:CDS	35.0%
	CAAGATTTAACGGAGCTTAA+TGG	+	chr2.3:46415881-46415900	MS.gene062141:CDS	35.0%
	GAAATGGAGAGAATGAGAAT+GGG	+	chr2.3:46415846-46415865	MS.gene062141:CDS	35.0%
	GAAATTAAGACACTTTGGTG+TGG	-	chr2.3:46415584-46415603	None:intergenic	35.0%
	GCCTTCAACTACTTAAACTT+TGG	+	chr2.3:46415392-46415411	MS.gene062141:CDS	35.0%
	GTACACGTGTCAAGATTTAA+CGG	+	chr2.3:46415871-46415890	MS.gene062141:CDS	35.0%
	GTTGCATCACAAATGAATCA+TGG	+	chr2.3:46415948-46415967	MS.gene062141:CDS	35.0%
	GTTTACGTTGTATTACCAAG+AGG	+	chr2.3:46415700-46415719	MS.gene062141:CDS	35.0%
	TTCTCAACAACTTATGGACT+TGG	+	chr2.3:46415426-46415445	MS.gene062141:CDS	35.0%
!	AAAAGACATACATCTGGAAC+TGG	-	chr2.3:46415975-46415994	None:intergenic	35.0%
!	AAGGCATTAAGGTTGAGAAA+TGG	+	chr2.3:46415830-46415849	MS.gene062141:CDS	35.0%
!	TACATTTTCATCTGTAACGG+TGG	-	chr2.3:46415606-46415625	None:intergenic	35.0%
!!	AACGTGGTTAAATTTTGGGA+CGG	+	chr2.3:46415905-46415924	MS.gene062141:CDS	35.0%
	CAACGTTGGAATCTAACAAG+AGG	-	chr2.3:46415364-46415383	None:intergenic	40.0%
	CGGTGGAAATTAAGACACTT+TGG	-	chr2.3:46415589-46415608	None:intergenic	40.0%
	CTTGTTAGATTCCAACGTTG+AGG	+	chr2.3:46415364-46415383	MS.gene062141:CDS	40.0%
	GTTTCAGTAAAACCGGATCA+AGG	+	chr2.3:46415518-46415537	MS.gene062141:CDS	40.0%
	TAATTGTTACGGGCAATTGC+CGG	+	chr2.3:46415754-46415773	MS.gene062141:CDS	40.0%
!	TGATCCGGTTTTACTGAAAC+CGG	-	chr2.3:46415518-46415537	None:intergenic	40.0%
!!	AATGTTGGGAGAAGGCATTA+AGG	+	chr2.3:46415819-46415838	MS.gene062141:CDS	40.0%
!!!	TGGTGTGGTTTTATTTCCAG+CGG	-	chr2.3:46415569-46415588	None:intergenic	40.0%
!!!	TGGTTAAATTTTGGGACGGT+GGG	+	chr2.3:46415909-46415928	MS.gene062141:CDS	40.0%
	AACGGAGCTTAATGGAAACG+TGG	+	chr2.3:46415889-46415908	MS.gene062141:CDS	45.0%
	AATCTTCCAGAAGCTGAGAG+CGG	-	chr2.3:46415465-46415484	None:intergenic	45.0%
	AGACCCGGTTTCAGTAAAAC+CGG	+	chr2.3:46415511-46415530	MS.gene062141:CDS	45.0%
	ATCCACATTGCATGTCCTCT+TGG	-	chr2.3:46415718-46415737	None:intergenic	45.0%
	ATGGAGAGAATGAGAATGGG+TGG	+	chr2.3:46415849-46415868	MS.gene062141:CDS	45.0%
	GAAACCGGGTCTAGTGATTT+GGG	-	chr2.3:46415503-46415522	None:intergenic	45.0%
	GAAACTGAGACACCTTGATC+CGG	-	chr2.3:46415533-46415552	None:intergenic	45.0%
	TACCAAGAGGACATGCAATG+TGG	+	chr2.3:46415713-46415732	MS.gene062141:CDS	45.0%
	TGAAACCGGGTCTAGTGATT+TGG	-	chr2.3:46415504-46415523	None:intergenic	45.0%
	TTGTCCCAAATCACTAGACC+CGG	+	chr2.3:46415496-46415515	MS.gene062141:CDS	45.0%
!	AAGGTGAGTGGTGGAAATGT+TGG	+	chr2.3:46415804-46415823	MS.gene062141:CDS	45.0%
!	AATTGCCGGTTGTTAAAGGG+TGG	+	chr2.3:46415768-46415787	MS.gene062141:CDS	45.0%
!	AGGTGAGTGGTGGAAATGTT+GGG	+	chr2.3:46415805-46415824	MS.gene062141:CDS	45.0%
!	ATTGCCGGTTGTTAAAGGGT+GGG	+	chr2.3:46415769-46415788	MS.gene062141:CDS	45.0%
!	GATCAAGGTGTCTCAGTTTC+CGG	+	chr2.3:46415533-46415552	MS.gene062141:CDS	45.0%
!	GATCCGGTTTTACTGAAACC+GGG	-	chr2.3:46415517-46415536	None:intergenic	45.0%
!	GGCAATTGCCGGTTGTTAAA+GGG	+	chr2.3:46415765-46415784	MS.gene062141:CDS	45.0%
!	GTCTAGTGATTTGGGACAAC+CGG	-	chr2.3:46415495-46415514	None:intergenic	45.0%
!	TTCTGGAAGATTCGCTCATC+CGG	+	chr2.3:46415473-46415492	MS.gene062141:CDS	45.0%
!!	TTGATGATGTTGACGGTGTG+AGG	+	chr2.3:46415669-46415688	MS.gene062141:CDS	45.0%
!!!	GTGGTTAAATTTTGGGACGG+TGG	+	chr2.3:46415908-46415927	MS.gene062141:CDS	45.0%
	AACCGGAAGTTGAAGGTGAG+TGG	+	chr2.3:46415792-46415811	MS.gene062141:CDS	50.0%
	ATGTTGACGGTGTGAGGAAG+GGG	+	chr2.3:46415675-46415694	MS.gene062141:CDS	50.0%
	CACCACTCACCTTCAACTTC+CGG	-	chr2.3:46415797-46415816	None:intergenic	50.0%
	GAAGGCTAAAGCCTCAACGT+TGG	-	chr2.3:46415378-46415397	None:intergenic	50.0%
	GATGTTGACGGTGTGAGGAA+GGG	+	chr2.3:46415674-46415693	MS.gene062141:CDS	50.0%
	GGTTCCCACCCTTTAACAAC+CGG	-	chr2.3:46415776-46415795	None:intergenic	50.0%
	GGTTGTTAAAGGGTGGGAAC+CGG	+	chr2.3:46415775-46415794	MS.gene062141:CDS	50.0%
	TGATGTTGACGGTGTGAGGA+AGG	+	chr2.3:46415673-46415692	MS.gene062141:CDS	50.0%
	TTCCAGCGGAGATGTAACAC+CGG	-	chr2.3:46415555-46415574	None:intergenic	50.0%
!	GGGCAATTGCCGGTTGTTAA+AGG	+	chr2.3:46415764-46415783	MS.gene062141:CDS	50.0%
!	GTGGTGGAAATGTTGGGAGA+AGG	+	chr2.3:46415811-46415830	MS.gene062141:CDS	50.0%
!!	GACGGTGGGTTAACGTTTGT+TGG	+	chr2.3:46415923-46415942	MS.gene062141:CDS	50.0%
	CGGAAGTTGAAGGTGAGTGG+TGG	+	chr2.3:46415795-46415814	MS.gene062141:CDS	55.0%
!!	TTCCGGTGTTACATCTCCGC+TGG	+	chr2.3:46415550-46415569	MS.gene062141:CDS	55.0%
	CTTCCAGAAGCTGAGAGCGG+CGG	-	chr2.3:46415462-46415481	None:intergenic	60.0%
	GGGTGGGAACCGGAAGTTGA+AGG	+	chr2.3:46415785-46415804	MS.gene062141:CDS	60.0%
	TCACCGCCGCTCTCAGCTTC+TGG	+	chr2.3:46415456-46415475	MS.gene062141:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	46415356	46416000	46415356	ID=MS.gene062141
chr2.3	mRNA	46415356	46416000	46415356	ID=MS.gene062141.t1;Parent=MS.gene062141
chr2.3	exon	46415356	46416000	46415356	ID=MS.gene062141.t1.exon1;Parent=MS.gene062141.t1
chr2.3	CDS	46415356	46416000	46415356	ID=cds.MS.gene062141.t1;Parent=MS.gene062141.t1

Gene Sequence

>MS.gene062141

ATGAACCTCTTGTTAGATTCCAACGTTGAGGCTTTAGCCTTCAACTACTTAAACTTTGGTTTAATCACAATTCTCAACAACTTATGGACTTGGCTTGCTATCACCGCCGCTCTCAGCTTCTGGAAGATTCGCTCATCCGGTTGTCCCAAATCACTAGACCCGGTTTCAGTAAAACCGGATCAAGGTGTCTCAGTTTCCGGTGTTACATCTCCGCTGGAAATAAAACCACACCAAAGTGTCTTAATTTCCACCGTTACAGATGAAAATGTAACGTTGAAAACTGAGACACCTCGTAAAACATCATCAAATGATTTTGATGATGTTGACGGTGTGAGGAAGGGGAAGTTTACGTTGTATTACCAAGAGGACATGCAATGTGGATTCAACAAGAGTAGTAGTAATTGTTACGGGCAATTGCCGGTTGTTAAAGGGTGGGAACCGGAAGTTGAAGGTGAGTGGTGGAAATGTTGGGAGAAGGCATTAAGGTTGAGAAATGGAGAGAATGAGAATGGGTGGTACACGTGTCAAGATTTAACGGAGCTTAATGGAAACGTGGTTAAATTTTGGGACGGTGGGTTAACGTTTGTTGGTAGTTGCATCACAAATGAATCATGGTCCAGTTCCAGATGTATGTCTTTTGAATGA

Protein sequence

>MS.gene062141.t1

MNLLLDSNVEALAFNYLNFGLITILNNLWTWLAITAALSFWKIRSSGCPKSLDPVSVKPDQGVSVSGVTSPLEIKPHQSVLISTVTDENVTLKTETPRKTSSNDFDDVDGVRKGKFTLYYQEDMQCGFNKSSSNCYGQLPVVKGWEPEVEGEWWKCWEKALRLRNGENENGWYTCQDLTELNGNVVKFWDGGLTFVGSCITNESWSSSRCMSFE