AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062300.t1 MTR_5g092570 96.107 745 29 0 1 745 1 745 0.0 1482
MS.gene062300.t1 MTR_3g051300 67.600 750 224 4 1 744 1 737 0.0 1029
MS.gene062300.t1 MTR_2g102180 51.469 783 337 7 1 744 1 779 0.0 800
MS.gene062300.t1 MTR_4g012050 50.828 785 343 8 1 746 1 781 0.0 770
MS.gene062300.t1 MTR_6g033955 34.954 432 267 4 4 427 10 435 1.05e-81 274
MS.gene062300.t1 MTR_4g063710 35.831 427 265 4 4 427 10 430 1.61e-81 273
MS.gene062300.t1 MTR_7g099680 36.066 427 264 4 4 427 10 430 4.03e-81 272
MS.gene062300.t1 MTR_7g024390 35.831 427 265 4 4 427 10 430 6.60e-81 271
MS.gene062300.t1 MTR_4g130540 31.751 611 370 14 4 582 9 604 3.47e-80 270
MS.gene062300.t1 MTR_4g063720 35.363 427 267 4 4 427 10 430 8.70e-80 269
MS.gene062300.t1 MTR_6g033760 35.681 426 265 4 4 426 10 429 3.21e-79 266
MS.gene062300.t1 MTR_7g024580 35.363 427 267 4 4 427 10 430 3.29e-78 265
MS.gene062300.t1 MTR_6g033820 34.086 443 276 5 4 436 10 446 8.57e-78 264
MS.gene062300.t1 MTR_8g099945 29.349 661 374 16 2 646 37 620 1.07e-75 258
MS.gene062300.t1 MTR_3g081170 27.307 802 520 21 2 743 25 823 1.71e-75 262
MS.gene062300.t1 MTR_8g099795 29.141 652 372 15 2 637 37 614 2.08e-75 257
MS.gene062300.t1 MTR_6g033870 34.028 432 270 5 4 426 11 436 3.10e-75 257
MS.gene062300.t1 MTR_6g033850 34.118 425 271 4 4 425 10 428 8.52e-75 256
MS.gene062300.t1 MTR_7g026030 34.033 429 271 6 4 427 10 431 6.49e-73 249
MS.gene062300.t1 MTR_8g032650 34.186 430 271 6 4 428 10 432 1.40e-72 249
MS.gene062300.t1 MTR_2g437440 34.186 430 271 6 4 428 10 432 3.26e-72 247
MS.gene062300.t1 MTR_2g038260 32.944 428 278 5 4 427 10 432 4.26e-72 247
MS.gene062300.t1 MTR_2g032550 32.990 485 294 9 4 463 11 489 1.26e-71 246
MS.gene062300.t1 MTR_7g451380 32.136 501 314 10 4 491 10 497 8.90e-71 243
MS.gene062300.t1 MTR_7g025770 33.255 427 275 7 2 424 8 428 1.17e-70 243
MS.gene062300.t1 MTR_7g026090 32.383 491 284 10 4 462 10 484 1.23e-70 243
MS.gene062300.t1 MTR_7g078190 33.721 430 274 7 4 428 10 433 1.53e-70 243
MS.gene062300.t1 MTR_7g025720 34.186 430 271 6 4 428 10 432 1.63e-70 243
MS.gene062300.t1 MTR_2g017730 30.375 507 316 9 4 489 9 499 2.72e-70 242
MS.gene062300.t1 MTR_7g025760 31.546 485 298 8 4 462 10 486 3.05e-70 242
MS.gene062300.t1 MTR_5g066800 32.955 440 274 7 4 428 10 443 6.18e-70 241
MS.gene062300.t1 MTR_7g025740 33.412 425 273 6 4 424 10 428 7.80e-70 242
MS.gene062300.t1 MTR_7g025790 33.645 428 274 5 4 427 10 431 7.85e-70 241
MS.gene062300.t1 MTR_7g024030 33.102 432 279 5 4 431 10 435 1.74e-69 240
MS.gene062300.t1 MTR_7g029415 33.100 429 278 5 4 428 17 440 5.06e-69 239
MS.gene062300.t1 MTR_7g021440 33.333 429 274 7 4 427 10 431 1.13e-68 238
MS.gene062300.t1 MTR_7g026170 33.100 429 278 5 4 428 10 433 8.74e-68 236
MS.gene062300.t1 MTR_7g078210 32.093 430 280 7 4 428 10 432 1.11e-67 235
MS.gene062300.t1 MTR_2g032580 31.635 471 301 8 4 462 10 471 2.08e-67 234
MS.gene062300.t1 MTR_7g026110 33.409 440 272 10 4 434 10 437 5.04e-67 233
MS.gene062300.t1 MTR_7g025620 32.028 434 283 6 2 430 8 434 7.18e-67 233
MS.gene062300.t1 MTR_4g079590 31.702 429 283 5 4 428 10 432 1.66e-66 232
MS.gene062300.t1 MTR_2g010020 31.373 510 297 10 3 491 56 533 2.43e-66 233
MS.gene062300.t1 MTR_2g010020 31.373 510 297 10 3 491 57 534 2.50e-66 233
MS.gene062300.t1 MTR_2g040370 34.828 379 243 2 4 381 1030 1405 2.71e-66 240
MS.gene062300.t1 MTR_2g040370 32.240 366 243 3 4 367 1414 1776 9.16e-56 208
MS.gene062300.t1 MTR_2g040370 34.828 379 243 2 4 381 1030 1405 3.83e-66 239
MS.gene062300.t1 MTR_2g040370 32.240 366 243 3 4 367 1414 1776 1.22e-55 207
MS.gene062300.t1 MTR_7g026130 31.935 429 285 5 4 428 10 435 8.59e-66 232
MS.gene062300.t1 MTR_2g022050 33.023 430 276 6 4 428 10 432 2.20e-65 229
MS.gene062300.t1 MTR_7g025820 30.247 486 303 8 4 462 10 486 9.67e-65 226
MS.gene062300.t1 MTR_4g092920 32.333 433 267 7 3 428 53 466 1.22e-64 228
MS.gene062300.t1 MTR_7g025630 33.100 429 263 6 4 428 10 418 9.24e-64 224
MS.gene062300.t1 MTR_2g040160 33.676 389 254 2 4 391 1110 1495 1.28e-63 232
MS.gene062300.t1 MTR_7g011590 31.469 429 272 5 4 428 10 420 1.22e-62 221
MS.gene062300.t1 MTR_2g038270 30.208 480 313 8 3 469 9 479 1.31e-62 221
MS.gene062300.t1 MTR_2g040260 33.162 389 256 2 4 391 1039 1424 1.32e-62 229
MS.gene062300.t1 MTR_7g026140 30.536 429 273 6 4 428 10 417 6.72e-62 219
MS.gene062300.t1 MTR_8g103930 31.674 442 263 11 4 429 12 430 1.30e-60 216
MS.gene062300.t1 MTR_2g032160 33.424 368 239 4 4 367 10 375 2.92e-60 211
MS.gene062300.t1 MTR_7g025260 30.580 448 275 8 42 462 20 458 1.02e-59 213
MS.gene062300.t1 MTR_4g027145 31.293 441 282 6 4 428 10 445 1.36e-59 213
MS.gene062300.t1 MTR_7g451360 32.918 401 234 5 4 400 10 379 7.40e-59 209
MS.gene062300.t1 MTR_2g040350 33.249 394 255 3 3 391 1038 1428 8.03e-57 211
MS.gene062300.t1 MTR_2g040350 33.249 394 255 3 3 391 820 1210 1.61e-56 210
MS.gene062300.t1 MTR_2g040220 32.741 394 260 5 4 394 1019 1410 2.87e-56 209
MS.gene062300.t1 MTR_1g017130 29.535 430 253 7 4 428 10 394 3.24e-56 203
MS.gene062300.t1 MTR_6g033800 33.236 343 222 3 84 425 1 337 5.39e-55 199
MS.gene062300.t1 MTR_1g077230 36.000 275 171 3 4 275 10 282 2.81e-52 183
MS.gene062300.t1 MTR_7g035155 29.439 428 241 6 4 428 10 379 4.76e-52 191
MS.gene062300.t1 MTR_2g005690 27.949 551 354 10 3 528 71 603 7.96e-52 192
MS.gene062300.t1 MTR_7g074460 30.000 430 271 8 4 428 10 414 9.46e-50 184
MS.gene062300.t1 MTR_7g007000 28.037 428 256 6 4 428 10 388 8.59e-49 182
MS.gene062300.t1 MTR_4g063720 30.211 427 218 12 4 427 10 359 1.19e-47 178
MS.gene062300.t1 MTR_6g034005 31.728 353 227 6 74 424 2 342 7.96e-45 172
MS.gene062300.t1 MTR_4g092920 30.769 351 218 6 84 428 1 332 2.24e-44 168
MS.gene062300.t1 MTR_1g041565 27.970 404 233 8 2 401 4 353 5.13e-41 158
MS.gene062300.t1 MTR_7g026080 34.894 235 150 3 4 236 10 243 2.95e-39 146
MS.gene062300.t1 MTR_6g033810 29.677 310 166 3 100 406 30 290 5.53e-34 132
MS.gene062300.t1 MTR_4g088515 30.242 248 152 3 4 249 277 505 5.71e-31 129
MS.gene062300.t1 MTR_2g031670 31.818 220 149 1 23 242 53 271 5.96e-31 125
MS.gene062300.t1 MTR_1207s0010 36.872 179 111 2 100 278 1 177 7.85e-30 116
MS.gene062300.t1 MTR_5g097190 32.000 175 106 3 4 176 159 322 2.59e-22 100
MS.gene062300.t1 MTR_7g026180 38.136 118 73 0 70 187 23 140 2.54e-20 93.6
MS.gene062300.t1 MTR_4g115780 25.328 458 291 15 4 429 141 579 3.18e-20 96.3
MS.gene062300.t1 MTR_5g006180 24.330 448 285 16 4 429 30 445 1.71e-19 93.2
MS.gene062300.t1 MTR_6g478100 26.906 223 148 3 30 252 10 217 2.13e-19 90.5
MS.gene062300.t1 MTR_6g033910 29.310 232 106 4 44 273 3 178 2.77e-19 86.7
MS.gene062300.t1 MTR_7g026050 31.138 167 112 3 238 401 1 167 6.47e-18 86.7
MS.gene062300.t1 MTR_4g107920 23.831 449 283 19 4 425 21 437 1.68e-13 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062300.t1 AT1G11660 61.714 747 266 6 1 744 1 730 0.0 934
MS.gene062300.t1 AT1G11660 61.714 747 266 6 1 744 1 730 0.0 934
MS.gene062300.t1 AT1G11660 61.714 747 266 6 1 744 1 730 0.0 934
MS.gene062300.t1 AT1G11660 60.282 710 262 6 38 744 1 693 0.0 866
MS.gene062300.t1 AT1G79930 51.403 784 339 7 1 746 1 780 0.0 803
MS.gene062300.t1 AT1G79930 51.531 784 338 7 1 746 1 780 0.0 803
MS.gene062300.t1 AT1G79920 53.467 649 267 6 1 618 1 645 0.0 702
MS.gene062300.t1 AT1G79920 53.621 649 266 6 1 618 1 645 0.0 699
MS.gene062300.t1 AT1G79920 53.621 649 266 6 1 618 1 645 0.0 699
MS.gene062300.t1 AT1G79920 53.621 649 266 6 1 618 1 645 0.0 699
MS.gene062300.t1 AT1G16030 35.831 427 265 4 4 427 9 429 3.14e-82 275
MS.gene062300.t1 AT5G02500 35.129 427 268 4 4 427 10 430 1.64e-79 268
MS.gene062300.t1 AT5G42020 29.056 678 390 17 2 663 36 638 5.76e-79 267
MS.gene062300.t1 AT5G42020 28.909 678 391 16 2 663 41 643 1.03e-78 266
MS.gene062300.t1 AT3G09440 35.597 427 266 4 4 427 10 430 1.17e-78 266
MS.gene062300.t1 AT3G09440 35.597 427 266 4 4 427 10 430 1.17e-78 266
MS.gene062300.t1 AT3G09440 35.597 427 266 4 4 427 10 430 1.17e-78 266
MS.gene062300.t1 AT3G09440 35.597 427 266 4 4 427 10 430 1.17e-78 266
MS.gene062300.t1 AT3G12580 35.129 427 268 4 4 427 10 430 1.75e-78 265
MS.gene062300.t1 AT5G02500 36.412 379 239 2 4 381 10 387 4.76e-77 258
MS.gene062300.t1 AT1G56410 34.426 427 271 4 4 427 10 430 5.20e-77 260
MS.gene062300.t1 AT5G02490 34.660 427 270 4 4 427 10 430 8.09e-77 261
MS.gene062300.t1 AT4G16660 26.884 796 518 20 2 739 25 814 4.74e-76 263
MS.gene062300.t1 AT4G16660 26.884 796 518 21 2 739 49 838 7.42e-76 263
MS.gene062300.t1 AT5G42020 34.571 431 268 6 2 428 36 456 2.23e-74 253
MS.gene062300.t1 AT5G28540 34.571 431 268 6 2 428 36 456 3.44e-74 254
MS.gene062300.t1 AT1G09080 34.419 430 269 5 2 428 41 460 1.54e-72 249
MS.gene062300.t1 AT1G09080 34.419 430 269 5 2 428 51 470 2.52e-72 249
MS.gene062300.t1 AT5G09590 33.025 433 264 7 3 428 59 472 2.37e-64 228
MS.gene062300.t1 AT4G37910 33.506 385 237 5 3 380 54 426 1.49e-62 223
MS.gene062300.t1 AT4G37910 33.506 385 237 5 3 380 54 426 1.49e-62 223
MS.gene062300.t1 AT5G49910 29.860 499 323 9 3 492 80 560 3.64e-55 202
MS.gene062300.t1 AT4G24280 30.588 510 303 11 3 491 80 559 5.21e-53 196
MS.gene062300.t1 AT2G32120 24.161 447 290 15 4 429 31 449 8.47e-22 100
MS.gene062300.t1 AT2G32120 24.161 447 290 15 4 429 31 449 8.47e-22 100

Find 135 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATATCCTGCTGCATTAAT+TGG 0.189410 5.1:-73967882 None:intergenic
CCATTTGATTCTGCACAAAT+TGG 0.200089 5.1:-73966622 None:intergenic
CTTGAGATACTGTCGATTTC+GGG 0.225683 5.1:-73965324 None:intergenic
CTTGGAATGGGTTGGCCTTT+TGG 0.230653 5.1:-73966655 None:intergenic
GAATCTTGCAACAGGATTAT+TGG 0.236459 5.1:+73966059 MS.gene062300:CDS
GCTGTTTCGGATTGTGTTAT+TGG 0.250913 5.1:+73965553 MS.gene062300:CDS
TCAACAAATGCAACATAAAT+AGG 0.268832 5.1:-73965724 None:intergenic
ATGTTGCATTTGTTGATATA+GGG 0.270095 5.1:+73965731 MS.gene062300:CDS
TCTCAAGTCAAGAGACTAAT+AGG 0.278061 5.1:+73965340 MS.gene062300:CDS
ACTTGAGATACTGTCGATTT+CGG 0.285988 5.1:-73965325 None:intergenic
AACATCCCTTGCTTCAGAAT+TGG 0.304791 5.1:-73967389 None:intergenic
GACTATAGCTGAAAATAATC+TGG 0.305513 5.1:+73965525 MS.gene062300:CDS
TTCTAAAACTCTTACACTTC+AGG 0.306898 5.1:+73966678 MS.gene062300:CDS
AGAGCTCTGATCCAGTATTT+TGG 0.311661 5.1:+73969045 MS.gene062300:CDS
TAATCTGGGGTCTGCTGTTT+CGG 0.313875 5.1:+73965540 MS.gene062300:CDS
TGACAATGCCATGCAAATTT+AGG 0.316149 5.1:-73967207 None:intergenic
GTCCCCTTTGGTACTTCATC+TGG 0.321615 5.1:-73966739 None:intergenic
CGTCTACGACGTGAGGAATA+AGG 0.322267 5.1:+73967948 MS.gene062300:CDS
GAGGAAAGTCCAATAGAAAA+AGG 0.323306 5.1:-73966589 None:intergenic
TCGGTTGAGTTAGTTGGTTC+AGG 0.333440 5.1:+73966147 MS.gene062300:CDS
TCAAGAATTCCATCTATAAC+TGG 0.334779 5.1:+73966171 MS.gene062300:CDS
TGAGTTGAAATGAGTGTAGT+AGG 0.338312 5.1:+73965139 None:intergenic
TATGTTGCATTTGTTGATAT+AGG 0.339071 5.1:+73965730 MS.gene062300:CDS
GACCGTTGATAAGATTTATT+CGG 0.353826 5.1:+73966128 MS.gene062300:CDS
TGCAAGTCAGTAAAGTATAA+TGG 0.358286 5.1:-73965580 None:intergenic
TTAAGGTCACTTTGAACATC+AGG 0.383646 5.1:-73965379 None:intergenic
CCGCGTCTGCCAATGCTTTG+TGG 0.386645 5.1:-73966101 None:intergenic
ACTGCAACTGGTCTTGGATA+TGG 0.387050 5.1:+73965670 MS.gene062300:CDS
GTTAATAATCCAGTTATAGA+TGG 0.390468 5.1:-73966180 None:intergenic
TCTGAACATTGAGTGTTTGA+TGG 0.391960 5.1:+73965993 MS.gene062300:CDS
GATTGCACTGCAACTGGTCT+TGG 0.392875 5.1:+73965664 MS.gene062300:CDS
ACCCCTGCTGTCGTCTGCTT+CGG 0.393509 5.1:+73965250 MS.gene062300:CDS
AATACTGGATCAGAGCTCTT+AGG 0.395679 5.1:-73969041 None:intergenic
CAAGCTACCAGAGATTTGTT+AGG 0.398032 5.1:+73968810 MS.gene062300:CDS
AAAAGACAAGAGGAACTCTT+TGG 0.399396 5.1:+73967918 MS.gene062300:CDS
ATATCCCTTGACCAAAATAC+TGG 0.400246 5.1:-73969056 None:intergenic
GAAAAGATAAGGCCAACATA+AGG 0.406128 5.1:+73967782 MS.gene062300:CDS
ATTCATGATTGCACTGCAAC+TGG 0.407995 5.1:+73965658 MS.gene062300:CDS
AGCTTCCGTGACTTCAGCCT+TGG 0.410602 5.1:-73967845 None:intergenic
CTCAGTCTCTAGCAGGCTCC+TGG 0.413544 5.1:-73968595 None:intergenic
TCCGAAAATTAGTTGCTTCA+CGG 0.413586 5.1:+73966765 MS.gene062300:CDS
GGGTAGTAATTTGCTCCGTT+TGG 0.417319 5.1:+73966699 MS.gene062300:CDS
CTTGATGCGGCAACAATTGT+GGG 0.417591 5.1:+73965616 MS.gene062300:CDS
ACTCACTTGCATTCAGTGTT+CGG 0.423314 5.1:-73966227 None:intergenic
AGCATTGCTAATATTTGAAC+CGG 0.425319 5.1:-73965487 None:intergenic
TCAAAGATCCGGTGAAAGTA+AGG 0.425329 5.1:+73967162 MS.gene062300:CDS
ATAATTATGCATTTATGAAA+AGG 0.427176 5.1:-73969130 None:intergenic
TCCTTGTACCGGTTCTCGAT+TGG 0.427655 5.1:-73968771 None:intergenic
AACTCCCGCCTTACTTTCAC+CGG 0.428179 5.1:-73967170 None:intergenic
AACATAAATAGGATCTCCTT+GGG 0.428347 5.1:-73965713 None:intergenic
CTAATGCCAGAGCGTGCAAT+AGG 0.430405 5.1:+73965914 MS.gene062300:CDS
GAATTTGAGAATCTTGCAAC+AGG 0.431706 5.1:+73966051 MS.gene062300:CDS
TCTCCGAAGCAGACGACAGC+AGG 0.435715 5.1:-73965253 None:intergenic
GATTCACTTGGAATACTTGA+AGG 0.440096 5.1:+73965447 MS.gene062300:CDS
AGTTCTGAGTGCAAACTTAG+AGG 0.443479 5.1:+73965966 MS.gene062300:CDS
CAACATAAATAGGATCTCCT+TGG 0.445317 5.1:-73965714 None:intergenic
TCCACAAGAAAATAAAGAAC+TGG 0.453448 5.1:+73968869 MS.gene062300:CDS
TCTATTGCAGCATTTGAAGC+TGG 0.455621 5.1:+73965772 MS.gene062300:CDS
CTATTGCAGCATTTGAAGCT+GGG 0.458304 5.1:+73965773 MS.gene062300:CDS
TCTAAAACTCTTACACTTCA+GGG 0.460042 5.1:+73966679 MS.gene062300:CDS
ATTTATTCGGTTGAGTTAGT+TGG 0.462887 5.1:+73966141 MS.gene062300:CDS
TTGTGGGGTTGAAGCCTTTG+AGG 0.467596 5.1:+73965632 MS.gene062300:CDS
TTTCTTCTTCATCCTTGTAC+CGG 0.473985 5.1:-73968782 None:intergenic
CTTTCACCGGATCTTTGAAA+AGG 0.475612 5.1:-73967157 None:intergenic
AAAAGATAAGGCCAACATAA+GGG 0.476060 5.1:+73967783 MS.gene062300:CDS
TCTTGATGCGGCAACAATTG+TGG 0.479142 5.1:+73965615 MS.gene062300:CDS
ATTGGACTTTCCTCGGATGA+AGG 0.482476 5.1:+73966598 MS.gene062300:CDS
ACTATAGCTGAAAATAATCT+GGG 0.490081 5.1:+73965526 MS.gene062300:CDS
ACGCAGCCTATTGCACGCTC+TGG 0.492570 5.1:-73965920 None:intergenic
AGACGACAGCAGGGGTTTCG+CGG 0.494442 5.1:-73965243 None:intergenic
CACAAAGCATTGGCAGACGC+GGG 0.495853 5.1:+73966102 MS.gene062300:CDS
GAGTTGAAATGAGTGTAGTA+GGG 0.498308 5.1:+73965140 None:intergenic
CCACAAAGCATTGGCAGACG+CGG 0.501135 5.1:+73966101 MS.gene062300:CDS
AATGCAACCTAACAAATCTC+TGG 0.506367 5.1:-73968817 None:intergenic
TGGTGGATCCAATCGAGAAC+CGG 0.506796 5.1:+73968763 MS.gene062300:CDS
AAAACTAGAAGATCTAAAAC+AGG 0.506886 5.1:+73968668 MS.gene062300:CDS
AGAACGCCAATTAATGCAGC+AGG 0.511677 5.1:+73967876 MS.gene062300:CDS
AAGAGAAGGATGGCATATCC+AGG 0.512112 5.1:+73968577 MS.gene062300:CDS
ATCAATCCAATTCTGAAGCA+AGG 0.513410 5.1:+73967383 MS.gene062300:CDS
CCAATTTGTGCAGAATCAAA+TGG 0.513496 5.1:+73966622 MS.gene062300:CDS
TTGCTGTAGCGAAGCAAGGT+GGG 0.527005 5.1:+73965191 MS.gene062300:CDS
CCATACACGTTCTCACTCAC+TGG 0.529441 5.1:-73967814 None:intergenic
GCCATTCCTCAGTCTCTAGC+AGG 0.534750 5.1:-73968602 None:intergenic
AGAATTGACCTAAATTTGCA+TGG 0.541563 5.1:+73967199 MS.gene062300:CDS
TGATGGTACAAGAAAAGATA+AGG 0.545253 5.1:+73967771 MS.gene062300:CDS
TCTGCCAATGCTTTGTGGCA+AGG 0.546595 5.1:-73966096 None:intergenic
TATTCCTTGCCACAAAGCAT+TGG 0.548281 5.1:+73966092 MS.gene062300:CDS
TCCAATCGAGAACCGGTACA+AGG 0.564187 5.1:+73968770 MS.gene062300:CDS
CCAGTGAGTGAGAACGTGTA+TGG 0.565004 5.1:+73967814 MS.gene062300:CDS
ATTCGCAAGTGAACAAGAGA+AGG 0.565617 5.1:+73968563 MS.gene062300:CDS
TATAGGGCACTGTGATACTC+AGG 0.566653 5.1:+73965747 MS.gene062300:CDS
GAACCTTCAAAAGTCTCGAA+AGG 0.570209 5.1:-73965409 None:intergenic
TCAATCCAATTCTGAAGCAA+GGG 0.574093 5.1:+73967384 MS.gene062300:CDS
AATCCAGATGAAGTACCAAA+GGG 0.577178 5.1:+73966736 MS.gene062300:CDS
TAAGGTCACTTTGAACATCA+GGG 0.580856 5.1:-73965378 None:intergenic
AGATCCGGTGAAAGTAAGGC+GGG 0.582264 5.1:+73967166 MS.gene062300:CDS
AGTGTAGTAGGGTTTGACAT+TGG 0.582938 5.1:+73965151 MS.gene062300:CDS
TAGCATAGAATGCTTCCAAA+CGG 0.583326 5.1:-73966714 None:intergenic
ATGTTAGTGGATTTATCAAG+AGG 0.583828 5.1:+73966025 MS.gene062300:CDS
CAAAGTATCAATGAGAAGTG+TGG 0.591810 5.1:+73967448 MS.gene062300:CDS
AAGTCAAGAGACTAATAGGA+AGG 0.593673 5.1:+73965344 MS.gene062300:CDS
CTCCGAAGCAGACGACAGCA+GGG 0.601158 5.1:-73965252 None:intergenic
AATGACCAAGGCTGAAGTCA+CGG 0.602995 5.1:+73967840 MS.gene062300:CDS
AACCGAATAAATCTTATCAA+CGG 0.608952 5.1:-73966130 None:intergenic
TAATCCAGATGAAGTACCAA+AGG 0.614435 5.1:+73966735 MS.gene062300:CDS
CAATCATGAATCAACCTCAA+AGG 0.626617 5.1:-73965646 None:intergenic
TCAGTTGATCGAAGATCGTG+TGG 0.631614 5.1:+73967336 MS.gene062300:intron
TGGAGTTAACAAAAGACAAG+AGG 0.632388 5.1:+73967908 MS.gene062300:CDS
GCCTGCTAGAGACTGAGGAA+TGG 0.638921 5.1:+73968601 MS.gene062300:CDS
TGGTACATGAACCCTTATGT+TGG 0.639214 5.1:-73967794 None:intergenic
GCAAGTGAACAAGAGAAGGA+TGG 0.642555 5.1:+73968567 MS.gene062300:CDS
GAGTTCTCTCTTAAATAGAG+AGG 0.643935 5.1:-73966202 None:intergenic
TGCTGTAGCGAAGCAAGGTG+GGG 0.645053 5.1:+73965192 MS.gene062300:CDS
CAGGAGCCTGCTAGAGACTG+AGG 0.645244 5.1:+73968596 MS.gene062300:CDS
TGTCGAGCATCGCATGTCTG+CGG 0.653671 5.1:+73968839 MS.gene062300:CDS
ATCAAACACTCAATGTTCAG+AGG 0.653757 5.1:-73965991 None:intergenic
GTGATTGCTGTAGCGAAGCA+AGG 0.661230 5.1:+73965187 MS.gene062300:CDS
AGTGCAATAAAGCAGAACAA+TGG 0.662448 5.1:+73968985 MS.gene062300:CDS
GGAGACTCATAAGTTTACAC+CGG 0.668038 5.1:+73965468 MS.gene062300:CDS
TCCGAAGCAGACGACAGCAG+GGG 0.672114 5.1:-73965251 None:intergenic
AGTCTTACGTCTACGACGTG+AGG 0.672698 5.1:+73967941 MS.gene062300:CDS
AAGATCCGGTGAAAGTAAGG+CGG 0.672893 5.1:+73967165 MS.gene062300:CDS
ATTGCTGTAGCGAAGCAAGG+TGG 0.680658 5.1:+73965190 MS.gene062300:CDS
AATGCAGCAGGATATAACAA+TGG 0.684049 5.1:+73967888 MS.gene062300:CDS
GTGAGTGAGAACGTGTATGG+TGG 0.684557 5.1:+73967817 MS.gene062300:CDS
ACAAATTGGACCTTCATCCG+AGG 0.687049 5.1:-73966608 None:intergenic
GAGACGAGCGTATCTTGATG+CGG 0.688738 5.1:+73965603 MS.gene062300:CDS
CTATAGCTGAAAATAATCTG+GGG 0.693850 5.1:+73965527 MS.gene062300:CDS
TTTCTTCAACTTCTCACACG+CGG 0.699317 5.1:-73965944 None:intergenic
GCAAGTGAGTGTGTAGCTCG+TGG 0.714591 5.1:+73966240 MS.gene062300:CDS
TTGATGCGGCAACAATTGTG+GGG 0.715157 5.1:+73965617 MS.gene062300:CDS
ACATAAATAGGATCTCCTTG+GGG 0.731223 5.1:-73965712 None:intergenic
CGTGTATGGTGGAATGACCA+AGG 0.733526 5.1:+73967828 MS.gene062300:CDS
ATCCAGATGAAGTACCAAAG+GGG 0.745954 5.1:+73966737 MS.gene062300:CDS
AACATACTTACAGAACGACG+TGG 0.752245 5.1:-73967475 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTATGTAATATATATCATA+TGG - chr5.1:73966357-73966376 None:intergenic 15.0%
!! TATCTTTATGAAATTAAACT+TGG - chr5.1:73966474-73966493 None:intergenic 15.0%
!!! GAGATATTACATAAATTTTA+TGG - chr5.1:73966542-73966561 None:intergenic 15.0%
!!! GATTTACTGATTTATATTTT+CGG + chr5.1:73966855-73966874 MS.gene062300:intron 15.0%
!!! TTTTTTTTGTCATCTTAATT+TGG + chr5.1:73966969-73966988 MS.gene062300:intron 15.0%
!! AAAGCCATGTATTAAATAAT+AGG - chr5.1:73968458-73968477 None:intergenic 20.0%
!! AAGATGACAAAAAAAAAACT+CGG - chr5.1:73966965-73966984 None:intergenic 20.0%
!! ACATTGAAGAGATTAAAATT+AGG - chr5.1:73967262-73967281 None:intergenic 20.0%
!! AGATATTCCATTTAAAAAAG+GGG - chr5.1:73967510-73967529 None:intergenic 20.0%
!! GAGATATTCCATTTAAAAAA+GGG - chr5.1:73967511-73967530 None:intergenic 20.0%
!! GTAATATATATCATATGGAT+AGG - chr5.1:73966352-73966371 None:intergenic 20.0%
!! TATGCCTATTATTTAATACA+TGG + chr5.1:73968451-73968470 MS.gene062300:intron 20.0%
!! TCAGATAAAATTTAGCTATA+CGG - chr5.1:73967578-73967597 None:intergenic 20.0%
!! TGAGATATTCCATTTAAAAA+AGG - chr5.1:73967512-73967531 None:intergenic 20.0%
!! TGTATAGAATAAAGAGAAAT+AGG - chr5.1:73966438-73966457 None:intergenic 20.0%
!!! ATTACATAAATTTTATGGAG+AGG - chr5.1:73966537-73966556 None:intergenic 20.0%
!!! GTAATATCTCTTATTTATTC+AGG + chr5.1:73966552-73966571 MS.gene062300:intron 20.0%
! AACCGAATAAATCTTATCAA+CGG - chr5.1:73966133-73966152 None:intergenic 25.0%
! AATAAGGGGAAAATATAGAA+GGG - chr5.1:73966889-73966908 None:intergenic 25.0%
! AATGTGGTTACATAGTAATA+AGG + chr5.1:73967041-73967060 MS.gene062300:intron 25.0%
! ACTATAGCTGAAAATAATCT+GGG + chr5.1:73965526-73965545 MS.gene062300:CDS 25.0%
! ATAGGCATAAAAGATAAAGA+AGG - chr5.1:73968440-73968459 None:intergenic 25.0%
! ATCTTAATAAGATACAGACA+TGG + chr5.1:73967983-73968002 MS.gene062300:intron 25.0%
! CAACAGAAAATACTACAAAA+AGG - chr5.1:73966505-73966524 None:intergenic 25.0%
! CTGATTCATGAATAAATGTA+AGG + chr5.1:73968323-73968342 MS.gene062300:intron 25.0%
! GGTTATATCAGTGATTAAAA+AGG + chr5.1:73967062-73967081 MS.gene062300:intron 25.0%
! GTTAATAATCCAGTTATAGA+TGG - chr5.1:73966183-73966202 None:intergenic 25.0%
! TAAGCATTATGACAATGTAT+AGG - chr5.1:73967105-73967124 None:intergenic 25.0%
! TATCAAATGAGAATGAGAAA+AGG + chr5.1:73968254-73968273 MS.gene062300:intron 25.0%
! TCAACAAATGCAACATAAAT+AGG - chr5.1:73965727-73965746 None:intergenic 25.0%
! TTAAACTAAGATAGTGAAAC+TGG + chr5.1:73968346-73968365 MS.gene062300:intron 25.0%
! TTTCAAATGTGAATGAGAAA+AGG + chr5.1:73968089-73968108 MS.gene062300:intron 25.0%
!! AAAACTAGAAGATCTAAAAC+AGG + chr5.1:73968668-73968687 MS.gene062300:CDS 25.0%
!! ATGTTGCATTTGTTGATATA+GGG + chr5.1:73965731-73965750 MS.gene062300:CDS 25.0%
!! GATTCTATTCCTTTTTCTAT+TGG + chr5.1:73966580-73966599 MS.gene062300:CDS 25.0%
!! TATGTTGCATTTGTTGATAT+AGG + chr5.1:73965730-73965749 MS.gene062300:CDS 25.0%
!!! CAATGATGAATTGATTTTCA+GGG + chr5.1:73968289-73968308 MS.gene062300:intron 25.0%
!!! TCAATGATGAATTGATTTTC+AGG + chr5.1:73968288-73968307 MS.gene062300:intron 25.0%
AAAAGATAAGGCCAACATAA+GGG + chr5.1:73967783-73967802 MS.gene062300:CDS 30.0%
AACATAAATAGGATCTCCTT+GGG - chr5.1:73965716-73965735 None:intergenic 30.0%
AGAATTGACCTAAATTTGCA+TGG + chr5.1:73967199-73967218 MS.gene062300:CDS 30.0%
AGCATTGCTAATATTTGAAC+CGG - chr5.1:73965490-73965509 None:intergenic 30.0%
ATAAGGGGAAAATATAGAAG+GGG - chr5.1:73966888-73966907 None:intergenic 30.0%
ATGACAAAAAAAAAACTCGG+AGG - chr5.1:73966962-73966981 None:intergenic 30.0%
ATGGATGAAAAAGATGTTAG+TGG + chr5.1:73966012-73966031 MS.gene062300:CDS 30.0%
ATTGCAGATATGATTCATGT+TGG + chr5.1:73967704-73967723 MS.gene062300:intron 30.0%
ATTTATTCGGTTGAGTTAGT+TGG + chr5.1:73966141-73966160 MS.gene062300:CDS 30.0%
CTATAGCTGAAAATAATCTG+GGG + chr5.1:73965527-73965546 MS.gene062300:CDS 30.0%
GAATAAGGGGAAAATATAGA+AGG - chr5.1:73966890-73966909 None:intergenic 30.0%
GACTATAGCTGAAAATAATC+TGG + chr5.1:73965525-73965544 MS.gene062300:CDS 30.0%
GATCAACTGAAGACAAATAA+AGG - chr5.1:73967326-73967345 None:intergenic 30.0%
GATGCAATACTTTGTTAACT+TGG + chr5.1:73966403-73966422 MS.gene062300:intron 30.0%
GTATGTTCTATATTTCCTGA+TGG + chr5.1:73968484-73968503 MS.gene062300:intron 30.0%
TAAATTATATGCAGTCTCTC+AGG + chr5.1:73967611-73967630 MS.gene062300:intron 30.0%
TAGAAAAAGGAATAGAATCC+TGG - chr5.1:73966579-73966598 None:intergenic 30.0%
TATTCTTTGACACGAAAAAC+AGG - chr5.1:73966294-73966313 None:intergenic 30.0%
TCAAGAATTCCATCTATAAC+TGG + chr5.1:73966171-73966190 MS.gene062300:CDS 30.0%
TCCACAAGAAAATAAAGAAC+TGG + chr5.1:73968869-73968888 MS.gene062300:CDS 30.0%
TGATGGTACAAGAAAAGATA+AGG + chr5.1:73967771-73967790 MS.gene062300:CDS 30.0%
TGCAAGTCAGTAAAGTATAA+TGG - chr5.1:73965583-73965602 None:intergenic 30.0%
TTATATCCTGCTGCATTAAT+TGG - chr5.1:73967885-73967904 None:intergenic 30.0%
TTATTAAATACCAGTCCATC+AGG - chr5.1:73968502-73968521 None:intergenic 30.0%
! ACATTTTGCAGCAAAGTTTA+AGG + chr5.1:73965867-73965886 MS.gene062300:CDS 30.0%
! ATTTTGTTCTGTCTCAGAAA+TGG - chr5.1:73967419-73967438 None:intergenic 30.0%
! GACCGTTGATAAGATTTATT+CGG + chr5.1:73966128-73966147 MS.gene062300:CDS 30.0%
! TATAAAACAGATTTTCCCCA+AGG + chr5.1:73965697-73965716 MS.gene062300:CDS 30.0%
! TCAACACTTTTACATTACGA+AGG - chr5.1:73968231-73968250 None:intergenic 30.0%
! TCTAAAACTCTTACACTTCA+GGG + chr5.1:73966679-73966698 MS.gene062300:CDS 30.0%
! TCTCGAAAGGAAACATTTTA+AGG - chr5.1:73965399-73965418 None:intergenic 30.0%
! TTCTTGTTGTGTTTCATGAA+AGG + chr5.1:73966924-73966943 MS.gene062300:intron 30.0%
! TTTTCGTGTCAAAGAATATG+AGG + chr5.1:73966296-73966315 MS.gene062300:CDS 30.0%
! TTTTGTTCTGTCTCAGAAAT+GGG - chr5.1:73967418-73967437 None:intergenic 30.0%
! TTTTTCTATTGGACTTTCCT+CGG + chr5.1:73966591-73966610 MS.gene062300:CDS 30.0%
!! ATGTTAGTGGATTTATCAAG+AGG + chr5.1:73966025-73966044 MS.gene062300:CDS 30.0%
!! TTCATATCTAGCTTTGACTT+TGG + chr5.1:73968040-73968059 MS.gene062300:intron 30.0%
!! TTCTAAAACTCTTACACTTC+AGG + chr5.1:73966678-73966697 MS.gene062300:CDS 30.0%
!!! AAGAGTTTTAGAACTTGGAA+TGG - chr5.1:73966671-73966690 None:intergenic 30.0%
!!! ACCAGTTCTTTATTTTCTTG+TGG - chr5.1:73968873-73968892 None:intergenic 30.0%
!!! AGAGTTTTAGAACTTGGAAT+GGG - chr5.1:73966670-73966689 None:intergenic 30.0%
!!! AGTGTAAGAGTTTTAGAACT+TGG - chr5.1:73966676-73966695 None:intergenic 30.0%
!!! TGATGAACCCCTTTTTTAAA+TGG + chr5.1:73967500-73967519 MS.gene062300:intron 30.0%
AAAAGACAAGAGGAACTCTT+TGG + chr5.1:73967918-73967937 MS.gene062300:CDS 35.0%
AAGTCAAGAGACTAATAGGA+AGG + chr5.1:73965344-73965363 MS.gene062300:CDS 35.0%
AATGCAACCTAACAAATCTC+TGG - chr5.1:73968820-73968839 None:intergenic 35.0%
AATGCAGCAGGATATAACAA+TGG + chr5.1:73967888-73967907 MS.gene062300:CDS 35.0%
ACATAAATAGGATCTCCTTG+GGG - chr5.1:73965715-73965734 None:intergenic 35.0%
AGCAGGAAAGAAACTGAATA+AGG - chr5.1:73966905-73966924 None:intergenic 35.0%
AGTGCAATAAAGCAGAACAA+TGG + chr5.1:73968985-73969004 MS.gene062300:CDS 35.0%
ATATCCCTTGACCAAAATAC+TGG - chr5.1:73969059-73969078 None:intergenic 35.0%
ATCAAACACTCAATGTTCAG+AGG - chr5.1:73965994-73966013 None:intergenic 35.0%
CAACATAAATAGGATCTCCT+TGG - chr5.1:73965717-73965736 None:intergenic 35.0%
CAATCATGAATCAACCTCAA+AGG - chr5.1:73965649-73965668 None:intergenic 35.0%
CAGGAAAGAAACTGAATAAG+GGG - chr5.1:73966903-73966922 None:intergenic 35.0%
CATTCTACACTGTATATAGC+TGG + chr5.1:73968743-73968762 MS.gene062300:intron 35.0%
CCAATTTGTGCAGAATCAAA+TGG + chr5.1:73966622-73966641 MS.gene062300:CDS 35.0%
CCATTTGATTCTGCACAAAT+TGG - chr5.1:73966625-73966644 None:intergenic 35.0%
GAAAAGATAAGGCCAACATA+AGG + chr5.1:73967782-73967801 MS.gene062300:CDS 35.0%
GAATCTTGCAACAGGATTAT+TGG + chr5.1:73966059-73966078 MS.gene062300:CDS 35.0%
GAATTTGAGAATCTTGCAAC+AGG + chr5.1:73966051-73966070 MS.gene062300:CDS 35.0%
GACTTGATGTAGCAAATGTT+AGG + chr5.1:73968146-73968165 MS.gene062300:intron 35.0%
GAGGAAAGTCCAATAGAAAA+AGG - chr5.1:73966592-73966611 None:intergenic 35.0%
GAGTTCTCTCTTAAATAGAG+AGG - chr5.1:73966205-73966224 None:intergenic 35.0%
GATTCACTTGGAATACTTGA+AGG + chr5.1:73965447-73965466 MS.gene062300:CDS 35.0%
GCAGGAAAGAAACTGAATAA+GGG - chr5.1:73966904-73966923 None:intergenic 35.0%
GTTCTACAGAGAAAGAAATG+TGG + chr5.1:73967025-73967044 MS.gene062300:intron 35.0%
TAGCATAGAATGCTTCCAAA+CGG - chr5.1:73966717-73966736 None:intergenic 35.0%
TCCGAAAATTAGTTGCTTCA+CGG + chr5.1:73966765-73966784 MS.gene062300:CDS 35.0%
TCTCAAGTCAAGAGACTAAT+AGG + chr5.1:73965340-73965359 MS.gene062300:CDS 35.0%
TCTGAACATTGAGTGTTTGA+TGG + chr5.1:73965993-73966012 MS.gene062300:CDS 35.0%
TGACAATGCCATGCAAATTT+AGG - chr5.1:73967210-73967229 None:intergenic 35.0%
TGGAGTTAACAAAAGACAAG+AGG + chr5.1:73967908-73967927 MS.gene062300:CDS 35.0%
TGTACCATCAGACTGTAAAA+AGG - chr5.1:73967761-73967780 None:intergenic 35.0%
TTTCTTCTTCATCCTTGTAC+CGG - chr5.1:73968785-73968804 None:intergenic 35.0%
! AATCCAGATGAAGTACCAAA+GGG + chr5.1:73966736-73966755 MS.gene062300:CDS 35.0%
! AATGGTGTACTTTTCCCAAA+AGG + chr5.1:73966640-73966659 MS.gene062300:CDS 35.0%
! ACCGTGAAGCAACTAATTTT+CGG - chr5.1:73966769-73966788 None:intergenic 35.0%
! ATGCTTTTGACAGAAGCTTA+GGG + chr5.1:73965815-73965834 MS.gene062300:CDS 35.0%
! ATTGGTCCTTTTCAAAGATC+CGG + chr5.1:73967151-73967170 MS.gene062300:CDS 35.0%
! CAAAGTATCAATGAGAAGTG+TGG + chr5.1:73967448-73967467 MS.gene062300:CDS 35.0%
! CTTGTTCACTTGCGAATTTT+TGG - chr5.1:73968560-73968579 None:intergenic 35.0%
! GAGAGAAGCAACGATTTTTA+GGG + chr5.1:73965272-73965291 MS.gene062300:CDS 35.0%
! GTCATCTTAATTTGGCTGTT+TGG + chr5.1:73966977-73966996 MS.gene062300:intron 35.0%
! TAAGGTCACTTTGAACATCA+GGG - chr5.1:73965381-73965400 None:intergenic 35.0%
! TAATCCAGATGAAGTACCAA+AGG + chr5.1:73966735-73966754 MS.gene062300:CDS 35.0%
! TCTCTTATTTATTCAGGTCC+AGG + chr5.1:73966558-73966577 MS.gene062300:intron 35.0%
! TTAAGGTCACTTTGAACATC+AGG - chr5.1:73965382-73965401 None:intergenic 35.0%
! TTCTATATTTCCTGATGGAC+TGG + chr5.1:73968489-73968508 MS.gene062300:intron 35.0%
! TTTCCTTTCGAGACTTTTGA+AGG + chr5.1:73965406-73965425 MS.gene062300:CDS 35.0%
!! ACTTGAGATACTGTCGATTT+CGG - chr5.1:73965328-73965347 None:intergenic 35.0%
!! ATCAATCCAATTCTGAAGCA+AGG + chr5.1:73967383-73967402 MS.gene062300:CDS 35.0%
!! TCAATCCAATTCTGAAGCAA+GGG + chr5.1:73967384-73967403 MS.gene062300:CDS 35.0%
!! TGAATTGATTTTCAGGGACT+TGG + chr5.1:73968295-73968314 MS.gene062300:intron 35.0%
!!! TGATCCAGTATTTTGGTCAA+GGG + chr5.1:73969052-73969071 MS.gene062300:CDS 35.0%
!!! TTTTAGAACTTGGAATGGGT+TGG - chr5.1:73966666-73966685 None:intergenic 35.0%
AACATACTTACAGAACGACG+TGG - chr5.1:73967478-73967497 None:intergenic 40.0%
AACATCCCTTGCTTCAGAAT+TGG - chr5.1:73967392-73967411 None:intergenic 40.0%
AATACTGGATCAGAGCTCTT+AGG - chr5.1:73969044-73969063 None:intergenic 40.0%
ACTCACTTGCATTCAGTGTT+CGG - chr5.1:73966230-73966249 None:intergenic 40.0%
AGATAGTGAAACTGGTGTCT+AGG + chr5.1:73968354-73968373 MS.gene062300:intron 40.0%
AGGACCAATCTGAATAGAAG+CGG - chr5.1:73967140-73967159 None:intergenic 40.0%
AGTGTAGTAGGGTTTGACAT+TGG + chr5.1:73965151-73965170 MS.gene062300:CDS 40.0%
ATTCATGATTGCACTGCAAC+TGG + chr5.1:73965658-73965677 MS.gene062300:CDS 40.0%
ATTCGCAAGTGAACAAGAGA+AGG + chr5.1:73968563-73968582 MS.gene062300:CDS 40.0%
CATGAAACACAACAAGAAGC+AGG - chr5.1:73966922-73966941 None:intergenic 40.0%
GAACCTTCAAAAGTCTCGAA+AGG - chr5.1:73965412-73965431 None:intergenic 40.0%
GAAGTATAGCACAGGAAACA+TGG - chr5.1:73968716-73968735 None:intergenic 40.0%
GATAGTGAAACTGGTGTCTA+GGG + chr5.1:73968355-73968374 MS.gene062300:intron 40.0%
GCACAAGATGATCGAAATTC+TGG - chr5.1:73966825-73966844 None:intergenic 40.0%
GGAGACTCATAAGTTTACAC+CGG + chr5.1:73965468-73965487 MS.gene062300:CDS 40.0%
GGGAAAATATAGAAGGGGAT+GGG - chr5.1:73966883-73966902 None:intergenic 40.0%
TATTCCTTGCCACAAAGCAT+TGG + chr5.1:73966092-73966111 MS.gene062300:CDS 40.0%
TCAAAGATCCGGTGAAAGTA+AGG + chr5.1:73967162-73967181 MS.gene062300:CDS 40.0%
TCTACACTGTATATAGCTGG+TGG + chr5.1:73968746-73968765 MS.gene062300:intron 40.0%
TGGTACATGAACCCTTATGT+TGG - chr5.1:73967797-73967816 None:intergenic 40.0%
TTTCTTCAACTTCTCACACG+CGG - chr5.1:73965947-73965966 None:intergenic 40.0%
! ATCCAGATGAAGTACCAAAG+GGG + chr5.1:73966737-73966756 MS.gene062300:CDS 40.0%
! CAAGCTACCAGAGATTTGTT+AGG + chr5.1:73968810-73968829 MS.gene062300:CDS 40.0%
! CATGCCTTTTTACAGTCTGA+TGG + chr5.1:73967754-73967773 MS.gene062300:intron 40.0%
! CATGCTTTTGACAGAAGCTT+AGG + chr5.1:73965814-73965833 MS.gene062300:CDS 40.0%
! CTATTGCAGCATTTGAAGCT+GGG + chr5.1:73965773-73965792 MS.gene062300:CDS 40.0%
! CTTTCACCGGATCTTTGAAA+AGG - chr5.1:73967160-73967179 None:intergenic 40.0%
! GAGAACAAGAAGTATAGCAC+AGG - chr5.1:73968724-73968743 None:intergenic 40.0%
! GCTGTTTCGGATTGTGTTAT+TGG + chr5.1:73965553-73965572 MS.gene062300:CDS 40.0%
! GGAGAGAAGCAACGATTTTT+AGG + chr5.1:73965271-73965290 MS.gene062300:CDS 40.0%
! TCTATTGCAGCATTTGAAGC+TGG + chr5.1:73965772-73965791 MS.gene062300:CDS 40.0%
! TGCTTTTGACAGAAGCTTAG+GGG + chr5.1:73965816-73965835 MS.gene062300:CDS 40.0%
! TTTGCCGCTTCTATTCAGAT+TGG + chr5.1:73967133-73967152 MS.gene062300:intron 40.0%
!! ACTTTTGAAGGTTCAGATGG+TGG + chr5.1:73965418-73965437 MS.gene062300:CDS 40.0%
!! AGAGCTCTGATCCAGTATTT+TGG + chr5.1:73969045-73969064 MS.gene062300:CDS 40.0%
!! AGTTCTGAGTGCAAACTTAG+AGG + chr5.1:73965966-73965985 MS.gene062300:CDS 40.0%
!! CTTGAGATACTGTCGATTTC+GGG - chr5.1:73965327-73965346 None:intergenic 40.0%
!! CTTTAGAACTTGTGCTGCAA+AGG + chr5.1:73966796-73966815 MS.gene062300:intron 40.0%
!! GAGACTTTTGAAGGTTCAGA+TGG + chr5.1:73965415-73965434 MS.gene062300:CDS 40.0%
!!! CTGATCCAGTATTTTGGTCA+AGG + chr5.1:73969051-73969070 MS.gene062300:CDS 40.0%
!!! TGGTGGCATTTTGATTCACT+TGG + chr5.1:73965435-73965454 MS.gene062300:CDS 40.0%
AAGAGAAGGATGGCATATCC+AGG + chr5.1:73968577-73968596 MS.gene062300:CDS 45.0%
AAGATCCGGTGAAAGTAAGG+CGG + chr5.1:73967165-73967184 MS.gene062300:CDS 45.0%
AATGACCAAGGCTGAAGTCA+CGG + chr5.1:73967840-73967859 MS.gene062300:CDS 45.0%
ACAAATTGGACCTTCATCCG+AGG - chr5.1:73966611-73966630 None:intergenic 45.0%
AGAACGCCAATTAATGCAGC+AGG + chr5.1:73967876-73967895 MS.gene062300:CDS 45.0%
AGCACAGGAAACATGGAGAT+CGG - chr5.1:73968709-73968728 None:intergenic 45.0%
ATTGGACTTTCCTCGGATGA+AGG + chr5.1:73966598-73966617 MS.gene062300:CDS 45.0%
CTTGATGCGGCAACAATTGT+GGG + chr5.1:73965616-73965635 MS.gene062300:CDS 45.0%
GCAAGTGAACAAGAGAAGGA+TGG + chr5.1:73968567-73968586 MS.gene062300:CDS 45.0%
GGGGAAAATATAGAAGGGGA+TGG - chr5.1:73966884-73966903 None:intergenic 45.0%
TCAGTTGATCGAAGATCGTG+TGG + chr5.1:73967336-73967355 MS.gene062300:intron 45.0%
TCTTGATGCGGCAACAATTG+TGG + chr5.1:73965615-73965634 MS.gene062300:CDS 45.0%
TTGATGCGGCAACAATTGTG+GGG + chr5.1:73965617-73965636 MS.gene062300:CDS 45.0%
! ATTTTCGGAGATGTCCCCTT+TGG - chr5.1:73966754-73966773 None:intergenic 45.0%
! GCTTTTGACAGAAGCTTAGG+GGG + chr5.1:73965817-73965836 MS.gene062300:CDS 45.0%
! TAATCTGGGGTCTGCTGTTT+CGG + chr5.1:73965540-73965559 MS.gene062300:CDS 45.0%
! TCGGTTGAGTTAGTTGGTTC+AGG + chr5.1:73966147-73966166 MS.gene062300:CDS 45.0%
!! ACTGCAACTGGTCTTGGATA+TGG + chr5.1:73965670-73965689 MS.gene062300:CDS 45.0%
!! GGGTAGTAATTTGCTCCGTT+TGG + chr5.1:73966699-73966718 MS.gene062300:CDS 45.0%
!! TATAGGGCACTGTGATACTC+AGG + chr5.1:73965747-73965766 MS.gene062300:CDS 45.0%
!!! CAACGATTTTTAGGGTCTGC+TGG + chr5.1:73965280-73965299 MS.gene062300:CDS 45.0%
!!! TTGGAATGGGTTGGCCTTTT+GGG - chr5.1:73966657-73966676 None:intergenic 45.0%
AACTCCCGCCTTACTTTCAC+CGG - chr5.1:73967173-73967192 None:intergenic 50.0%
AGATCCGGTGAAAGTAAGGC+GGG + chr5.1:73967166-73967185 MS.gene062300:CDS 50.0%
AGGGGGTAGAGACTTTGATG+AGG + chr5.1:73965834-73965853 MS.gene062300:CDS 50.0%
AGTCTTACGTCTACGACGTG+AGG + chr5.1:73967941-73967960 MS.gene062300:CDS 50.0%
ATTGCTGTAGCGAAGCAAGG+TGG + chr5.1:73965190-73965209 MS.gene062300:CDS 50.0%
CCAGTGAGTGAGAACGTGTA+TGG + chr5.1:73967814-73967833 MS.gene062300:CDS 50.0%
CCATACACGTTCTCACTCAC+TGG - chr5.1:73967817-73967836 None:intergenic 50.0%
CGTCTACGACGTGAGGAATA+AGG + chr5.1:73967948-73967967 MS.gene062300:CDS 50.0%
CTAATGCCAGAGCGTGCAAT+AGG + chr5.1:73965914-73965933 MS.gene062300:CDS 50.0%
GAGACGAGCGTATCTTGATG+CGG + chr5.1:73965603-73965622 MS.gene062300:CDS 50.0%
GTCCCCTTTGGTACTTCATC+TGG - chr5.1:73966742-73966761 None:intergenic 50.0%
GTGAGTGAGAACGTGTATGG+TGG + chr5.1:73967817-73967836 MS.gene062300:CDS 50.0%
GTGATTGCTGTAGCGAAGCA+AGG + chr5.1:73965187-73965206 MS.gene062300:CDS 50.0%
TCCAATCGAGAACCGGTACA+AGG + chr5.1:73968770-73968789 MS.gene062300:CDS 50.0%
TCTGCCAATGCTTTGTGGCA+AGG - chr5.1:73966099-73966118 None:intergenic 50.0%
TTGCTGTAGCGAAGCAAGGT+GGG + chr5.1:73965191-73965210 MS.gene062300:CDS 50.0%
TTGTGGGGTTGAAGCCTTTG+AGG + chr5.1:73965632-73965651 MS.gene062300:CDS 50.0%
! CGTGTATGGTGGAATGACCA+AGG + chr5.1:73967828-73967847 MS.gene062300:CDS 50.0%
! GATTGCACTGCAACTGGTCT+TGG + chr5.1:73965664-73965683 MS.gene062300:CDS 50.0%
! TGGTGGATCCAATCGAGAAC+CGG + chr5.1:73968763-73968782 MS.gene062300:CDS 50.0%
!! TCCTTGTACCGGTTCTCGAT+TGG - chr5.1:73968774-73968793 None:intergenic 50.0%
!!! CTTGGAATGGGTTGGCCTTT+TGG - chr5.1:73966658-73966677 None:intergenic 50.0%
AGCTTCCGTGACTTCAGCCT+TGG - chr5.1:73967848-73967867 None:intergenic 55.0%
GCAAGTGAGTGTGTAGCTCG+TGG + chr5.1:73966240-73966259 MS.gene062300:CDS 55.0%
GCCATTCCTCAGTCTCTAGC+AGG - chr5.1:73968605-73968624 None:intergenic 55.0%
GCCTGCTAGAGACTGAGGAA+TGG + chr5.1:73968601-73968620 MS.gene062300:CDS 55.0%
TGCTGTAGCGAAGCAAGGTG+GGG + chr5.1:73965192-73965211 MS.gene062300:CDS 55.0%
! TGTCGAGCATCGCATGTCTG+CGG + chr5.1:73968839-73968858 MS.gene062300:CDS 55.0%
!! CACAAAGCATTGGCAGACGC+GGG + chr5.1:73966102-73966121 MS.gene062300:CDS 55.0%
!! CCACAAAGCATTGGCAGACG+CGG + chr5.1:73966101-73966120 MS.gene062300:CDS 55.0%
ACCCCTGCTGTCGTCTGCTT+CGG + chr5.1:73965250-73965269 MS.gene062300:CDS 60.0%
ACGCAGCCTATTGCACGCTC+TGG - chr5.1:73965923-73965942 None:intergenic 60.0%
AGACGACAGCAGGGGTTTCG+CGG - chr5.1:73965246-73965265 None:intergenic 60.0%
CAGGAGCCTGCTAGAGACTG+AGG + chr5.1:73968596-73968615 MS.gene062300:CDS 60.0%
CCGCGTCTGCCAATGCTTTG+TGG - chr5.1:73966104-73966123 None:intergenic 60.0%
CTCAGTCTCTAGCAGGCTCC+TGG - chr5.1:73968598-73968617 None:intergenic 60.0%
CTCCGAAGCAGACGACAGCA+GGG - chr5.1:73965255-73965274 None:intergenic 60.0%
TCCGAAGCAGACGACAGCAG+GGG - chr5.1:73965254-73965273 None:intergenic 60.0%
TCTCCGAAGCAGACGACAGC+AGG - chr5.1:73965256-73965275 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 73965148 73969148 73965148 ID=MS.gene062300
chr5.1 mRNA 73965148 73969148 73965148 ID=MS.gene062300.t1;Parent=MS.gene062300
chr5.1 exon 73965148 73966317 73965148 ID=MS.gene062300.t1.exon1;Parent=MS.gene062300.t1
chr5.1 CDS 73965148 73966317 73965148 ID=cds.MS.gene062300.t1;Parent=MS.gene062300.t1
chr5.1 exon 73966574 73966786 73966574 ID=MS.gene062300.t1.exon2;Parent=MS.gene062300.t1
chr5.1 CDS 73966574 73966786 73966574 ID=cds.MS.gene062300.t1;Parent=MS.gene062300.t1
chr5.1 exon 73967151 73967246 73967151 ID=MS.gene062300.t1.exon3;Parent=MS.gene062300.t1
chr5.1 CDS 73967151 73967246 73967151 ID=cds.MS.gene062300.t1;Parent=MS.gene062300.t1
chr5.1 exon 73967340 73967486 73967340 ID=MS.gene062300.t1.exon4;Parent=MS.gene062300.t1
chr5.1 CDS 73967340 73967486 73967340 ID=cds.MS.gene062300.t1;Parent=MS.gene062300.t1
chr5.1 exon 73967769 73967969 73967769 ID=MS.gene062300.t1.exon5;Parent=MS.gene062300.t1
chr5.1 CDS 73967769 73967969 73967769 ID=cds.MS.gene062300.t1;Parent=MS.gene062300.t1
chr5.1 exon 73968543 73968689 73968543 ID=MS.gene062300.t1.exon6;Parent=MS.gene062300.t1
chr5.1 CDS 73968543 73968689 73968543 ID=cds.MS.gene062300.t1;Parent=MS.gene062300.t1
chr5.1 exon 73968762 73968890 73968762 ID=MS.gene062300.t1.exon7;Parent=MS.gene062300.t1
chr5.1 CDS 73968762 73968890 73968762 ID=cds.MS.gene062300.t1;Parent=MS.gene062300.t1
chr5.1 exon 73968975 73969148 73968975 ID=MS.gene062300.t1.exon8;Parent=MS.gene062300.t1
chr5.1 CDS 73968975 73969148 73968975 ID=cds.MS.gene062300.t1;Parent=MS.gene062300.t1
Gene Sequence

>MS.gene062300

ATGAGTGTAGTAGGGTTTGACATTGGTAATGAGAATTGTGTGATTGCTGTAGCGAAGCAAGGTGGGGTTGATGTTTTGTTGAATGATGAATCGAACCGCGAAACCCCTGCTGTCGTCTGCTTCGGAGAGAAGCAACGATTTTTAGGGTCTGCTGGCGCTGCTTCTGCTATGATGCACCCGAAATCGACAGTATCTCAAGTCAAGAGACTAATAGGAAGGAGATTTGATGACCCTGATGTTCAAAGTGACCTTAAAATGTTTCCTTTCGAGACTTTTGAAGGTTCAGATGGTGGCATTTTGATTCACTTGGAATACTTGAAGGAGACTCATAAGTTTACACCGGTTCAAATATTAGCAATGCTCTTTGCTCACTTGAAGACTATAGCTGAAAATAATCTGGGGTCTGCTGTTTCGGATTGTGTTATTGGAGTTCCATTATACTTTACTGACTTGCAGAGACGAGCGTATCTTGATGCGGCAACAATTGTGGGGTTGAAGCCTTTGAGGTTGATTCATGATTGCACTGCAACTGGTCTTGGATATGGTGTTTATAAAACAGATTTTCCCCAAGGAGATCCTATTTATGTTGCATTTGTTGATATAGGGCACTGTGATACTCAGGTTTCTATTGCAGCATTTGAAGCTGGGAAAATGAAGATACTTTCACATGCTTTTGACAGAAGCTTAGGGGGTAGAGACTTTGATGAGGTTCTATTTAGACATTTTGCAGCAAAGTTTAAGGAACAGTACGAAATTGATGTGTATTCTAATGCCAGAGCGTGCAATAGGCTGCGTGCCGCGTGTGAGAAGTTGAAGAAAGTTCTGAGTGCAAACTTAGAGGCACCTCTGAACATTGAGTGTTTGATGGATGAAAAAGATGTTAGTGGATTTATCAAGAGGAATGAATTTGAGAATCTTGCAACAGGATTATTGGATAGAATTTGTATTCCTTGCCACAAAGCATTGGCAGACGCGGGCTTGACCGTTGATAAGATTTATTCGGTTGAGTTAGTTGGTTCAGGTTCAAGAATTCCATCTATAACTGGATTATTAACCTCTCTATTTAAGAGAGAACTCAGCCGAACACTGAATGCAAGTGAGTGTGTAGCTCGTGGTTGTGCTCTTCAGTGTGCAATGCTCAGTCCTGTTTTTCGTGTCAAAGAATATGAGGTTTGAATTTAAACAAAGCACTGATGCTTATCCTATCCATATGATATATATTACATACTTGATTTTTGAGTTTATAGATTCTAGAGATGCAATACTTTGTTAACTTGGTTTAGATTACCTATTTCTCTTTATTCTATACAAATTGAGATATAGCCAAGTTTAATTTCATAAAGATATTATACATCCTTTTTGTAGTATTTTCTGTTGTCTTGTATTCCTCTCCATAAAATTTATGTAATATCTCTTATTTATTCAGGTCCAGGATTCTATTCCTTTTTCTATTGGACTTTCCTCGGATGAAGGTCCAATTTGTGCAGAATCAAATGGTGTACTTTTCCCAAAAGGCCAACCCATTCCAAGTTCTAAAACTCTTACACTTCAGGGTAGTAATTTGCTCCGTTTGGAAGCATTCTATGCTAATCCAGATGAAGTACCAAAGGGGACATCTCCGAAAATTAGTTGCTTCACGGTAAGTGTTCTTTAGAACTTGTGCTGCAAAGGTGTCCAGAATTTCGATCATCTTGTGCATATTTCTGTGATTTACTGATTTATATTTTCGGCTCCCATCCCCTTCTATATTTTCCCCTTATTCAGTTTCTTTCCTGCTTCTTGTTGTGTTTCATGAAAGGCTTGTAGTGATGCCTCCGAGTTTTTTTTTTGTCATCTTAATTTGGCTGTTTGGCACTCAAAAATTCTTATATAGTGACGTTCTACAGAGAAAGAAATGTGGTTACATAGTAATAAGGTTATATCAGTGATTAAAAAGGTTGATGTATTCTGCATGCCTATACATTGTCATAATGCTTATACATTTGTTTGCCGCTTCTATTCAGATTGGTCCTTTTCAAAGATCCGGTGAAAGTAAGGCGGGAGTTGAAGTTAGAATTGACCTAAATTTGCATGGCATTGTCAGTATTGAATCATCTACAGTAAGTAAGAAGCCTAATTTTAATCTCTTCAATGTTACATCATCAGTTCTTTTAGAGATCTAGCTACATACATGAACCTTTATTTGTCTTCAGTTGATCGAAGATCGTGTGGAAGATTCTGTTACAACGCGTGAGTATCAATCCAATTCTGAAGCAAGGGATGTTGAACCCATTTCTGAGACAGAACAAAATGATAATGAACAAAGTATCAATGAGAAGTGTGGATCTCCACGTCGTTCTGTAAGTATGTTGTTGATGAACCCCTTTTTTAAATGGAATATCTCAAATGTTAATGATTTCTTGCAAATCATTCTTCAAACATGAAAAACCGTATAGCTAAATTTTATCTGATCATTTATGATGTTAAATTATATGCAGTCTCTCAGGCTATAACAACTGCTCTTTAATATATCTGATAATCATATATGTTTATTTTTATTTTGTGAAGATTGTTTTGATTGCAGATATGATTCATGTTGGTGATTTGAGTAAATAAAGTGTTACTATCATGCCTTTTTACAGTCTGATGGTACAAGAAAAGATAAGGCCAACATAAGGGTTCATGTACCAGTGAGTGAGAACGTGTATGGTGGAATGACCAAGGCTGAAGTCACGGAAGCTCAAGAAAAAGAACGCCAATTAATGCAGCAGGATATAACAATGGAGTTAACAAAAGACAAGAGGAACTCTTTGGAGTCTTACGTCTACGACGTGAGGAATAAGGTATAGATTCAATATCTTAATAAGATACAGACATGGTTTTTGTGACTAGATAATTTCTATTGTCACACATTTCATATCTAGCTTTGACTTTTGCTATGATAATATGAAAGTATTGAATGTTTCAAATGTGAATGAGAAAAGGCTGATGTATATTCAATGATGATTTCGTTTTCAGTGACTTGATGTAGCAAATGTTAGGTAAAACTAAGATAATGAAGTTAGTGCAGTCACACATTTCATATCTAGCTTTGACTTTGGCCTTCGTAATGTAAAAGTGTTGAATGTATCAAATGAGAATGAGAAAAGGCTTCTGTATATTCAATGATGAATTGATTTTCAGGGACTTGGCTGTTCTGATTCATGAATAAATGTAAGGTTAAACTAAGATAGTGAAACTGGTGTCTAGGGATATTGTTTTATTCTGCTTTGTATCAAATCTGCATATGAAATGAAATTAAAATCTTTATCCTTCTTTATCTTTTATGCCTATTATTTAATACATGGCTTTATGTGTGTATGTTCTATATTTCCTGATGGACTGGTATTTAATAATTTGTGCATTTTTATGAATAGCTCTTCAACGAATACCAAAAATTCGCAAGTGAACAAGAGAAGGATGGCATATCCAGGAGCCTGCTAGAGACTGAGGAATGGCTTTACAGTGAACGCGATGATGAAACAGTACATGCTTATTTTGCAAAACTAGAAGATCTAAAACAGGTTATCTTTTTGTTCTCCGATCTCCATGTTTCCTGTGCTATACTTCTTGTTCTCATTCTACACTGTATATAGCTGGTGGATCCAATCGAGAACCGGTACAAGGATGAAGAAGAAAGAGTGCAAGCTACCAGAGATTTGTTAGGTTGCATTGTCGAGCATCGCATGTCTGCGGATTCTCTTCCACAAGAAAATAAAGAACTGGTACATTAAATAAGAGAATAAATATTATTTTGAGATGCAGCTCTTAATATCAGCTTTCAAGTAACAACAACTTTCATGTTTTAGATAATCGATGAGTGCAATAAAGCAGAACAATGGCTAAGACAAAAGACGCAACAACAAGATGCTTTGCCTAAGAGCTCTGATCCAGTATTTTGGTCAAGGGATATCAATAGCAAGACACAAGATTTGAACTTGTATGATTTTACTCTTCTGTTTTTCCTTTTCATAAATGCATAA
Protein sequence

>MS.gene062300.t1

MSVVGFDIGNENCVIAVAKQGGVDVLLNDESNRETPAVVCFGEKQRFLGSAGAASAMMHPKSTVSQVKRLIGRRFDDPDVQSDLKMFPFETFEGSDGGILIHLEYLKETHKFTPVQILAMLFAHLKTIAENNLGSAVSDCVIGVPLYFTDLQRRAYLDAATIVGLKPLRLIHDCTATGLGYGVYKTDFPQGDPIYVAFVDIGHCDTQVSIAAFEAGKMKILSHAFDRSLGGRDFDEVLFRHFAAKFKEQYEIDVYSNARACNRLRAACEKLKKVLSANLEAPLNIECLMDEKDVSGFIKRNEFENLATGLLDRICIPCHKALADAGLTVDKIYSVELVGSGSRIPSITGLLTSLFKRELSRTLNASECVARGCALQCAMLSPVFRVKEYEVQDSIPFSIGLSSDEGPICAESNGVLFPKGQPIPSSKTLTLQGSNLLRLEAFYANPDEVPKGTSPKISCFTIGPFQRSGESKAGVEVRIDLNLHGIVSIESSTLIEDRVEDSVTTREYQSNSEARDVEPISETEQNDNEQSINEKCGSPRRSSDGTRKDKANIRVHVPVSENVYGGMTKAEVTEAQEKERQLMQQDITMELTKDKRNSLESYVYDVRNKLFNEYQKFASEQEKDGISRSLLETEEWLYSERDDETVHAYFAKLEDLKQLVDPIENRYKDEEERVQATRDLLGCIVEHRMSADSLPQENKELIIDECNKAEQWLRQKTQQQDALPKSSDPVFWSRDINSKTQDLNLYDFTLLFFLFINA