AlfalfaGEDB | MS.gene062424

M. sativa cultivar XinJiangDaYe / MS.gene062424

KEGG: K00028
GO: GO:0000166 GO:0003674 GO:0005488 GO:0036094 GO:0048037 GO:0050662 GO:0051287 GO:0097159 GO:1901265 GO:1901363
PFAM: PF03949.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062424.t1	XP_003627605.1	92.9	168	12	0	16	183	414	581	2.30E-81	312.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062424.t1	Q8L7K9	78.6	168	36	0	16	183	417	584	8.3e-72	271.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062424.t1	G7LBU3	92.9	168	12	0	16	183	414	581	1.7e-81	312.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056174	MS.gene062424	0.803209	3.89E-49	-1.69E-46
MS.gene058294	MS.gene062424	0.801398	9.18E-49	-1.69E-46
MS.gene059584	MS.gene062424	0.851104	1.10E-60	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062424.t1	MTR_8g032040	92.857	168	12	0	16	183	414	581	6.00e-107	323
MS.gene062424.t1	MTR_7g076650	80.952	168	32	0	16	183	411	578	2.86e-92	285
MS.gene062424.t1	MTR_7g076650	66.019	206	32	1	16	183	411	616	1.07e-84	267
MS.gene062424.t1	MTR_8g009500	60.795	176	69	0	8	183	335	510	9.99e-74	236
MS.gene062424.t1	MTR_8g009500	60.795	176	69	0	8	183	429	604	7.06e-73	236
MS.gene062424.t1	MTR_7g076650	86.111	108	15	0	16	123	411	518	2.56e-60	200
MS.gene062424.t1	MTR_8g102620	46.053	152	80	2	16	167	416	565	6.01e-34	130
MS.gene062424.t1	MTR_3g110330	42.442	172	95	3	16	187	403	570	2.33e-30	120
MS.gene062424.t1	MTR_8g014390	40.437	183	105	3	5	187	402	580	7.34e-30	119
MS.gene062424.t1	MTR_2g101040	39.344	183	107	3	5	187	489	667	3.42e-29	117

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062424.t1	AT4G00570	78.571	168	36	0	16	183	417	584	1.62e-89	278
MS.gene062424.t1	AT2G13560	62.500	176	66	0	8	183	424	599	1.34e-75	243
MS.gene062424.t1	AT5G25880	44.767	172	91	3	16	187	413	580	5.45e-37	139
MS.gene062424.t1	AT5G25880	48.592	142	71	2	16	157	413	552	4.01e-35	133
MS.gene062424.t1	AT2G19900	44.509	173	90	4	16	187	307	474	2.88e-32	124
MS.gene062424.t1	AT2G19900	44.509	173	90	4	16	187	406	573	4.45e-32	125
MS.gene062424.t1	AT5G11670	43.023	172	94	3	16	187	413	580	9.52e-30	118
MS.gene062424.t1	AT1G79750	40.698	172	98	3	16	187	471	638	5.65e-28	114
MS.gene062424.t1	AT1G65430	43.810	105	13	1	186	244	310	414	6.83e-18	84.3
MS.gene062424.t1	AT2G31510	46.000	100	8	1	191	244	252	351	9.04e-17	80.9

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Find 67 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGTGTTAGATGGTTA+AGG	0.302490	7.3:+75241887	MS.gene062424:CDS
AAAAGTACACAACCACACAC+TGG	0.665666	7.3:+75241736	MS.gene062424:CDS
AAACTGTGTTAGATGGTTAA+GGG	0.396058	7.3:+75241888	MS.gene062424:CDS
AACTGTGTTAGATGGTTAAG+GGG	0.536559	7.3:+75241889	MS.gene062424:CDS
AATCGCCAGTCCTTTCACCA+TGG	0.713996	7.3:-75241017	None:intergenic
ACAAACAAACCATTCAAGGT+GGG	0.617272	7.3:-75239536	None:intergenic
ACAACTTAAGTTCATAACCG+AGG	0.683597	7.3:+75241480	MS.gene062424:CDS
ACATGCTATGCCTTGGTGCA+TGG	0.548401	7.3:+75240989	MS.gene062424:CDS
ACTGTGTTAGATGGTTAAGG+GGG	0.600365	7.3:+75241890	MS.gene062424:CDS
ACTTAAGTTGTGACTCAGGT+TGG	0.567637	7.3:-75241468	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTGTGTTAGATGGTTA+AGG	+	chr7.3:75241887-75241906	MS.gene062424:CDS	30.0%
AAACTGTGTTAGATGGTTAA+GGG	+	chr7.3:75241888-75241907	MS.gene062424:CDS	30.0%
AACCTTCTTAACCTAAAGAT+CGG	-	chr7.3:75239252-75239271	None:intergenic	30.0%
GAACATTACCTATTAACAGA+TGG	-	chr7.3:75240381-75240400	None:intergenic	30.0%
GACTTAAAAACTGTGTTAGA+TGG	+	chr7.3:75241881-75241900	MS.gene062424:CDS	30.0%
GTTCACAGTAGTTGAAATTA+TGG	+	chr7.3:75240704-75240723	MS.gene062424:intron	30.0%
TAATAGTTGAGATTCAGTTC+AGG	+	chr7.3:75239454-75239473	MS.gene062424:intron	30.0%
TCCATCTGCCAATAAAAAAA+AGG	-	chr7.3:75241427-75241446	None:intergenic	30.0%
TCCTTGTAAAATATCTTCCT+CGG	-	chr7.3:75240349-75240368	None:intergenic	30.0%
TTACATTTCAGAATTGGGTT+GGG	+	chr7.3:75239944-75239963	MS.gene062424:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	75239021	75241954	75239021	ID=MS.gene062424
chr7.3	mRNA	75239021	75241954	75239021	ID=MS.gene062424.t1;Parent=MS.gene062424
chr7.3	exon	75239021	75239069	75239021	ID=MS.gene062424.t1.exon1;Parent=MS.gene062424.t1
chr7.3	CDS	75239021	75239069	75239021	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75239291	75239325	75239291	ID=MS.gene062424.t1.exon2;Parent=MS.gene062424.t1
chr7.3	CDS	75239291	75239325	75239291	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75239476	75239548	75239476	ID=MS.gene062424.t1.exon3;Parent=MS.gene062424.t1
chr7.3	CDS	75239476	75239548	75239476	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75239643	75239729	75239643	ID=MS.gene062424.t1.exon4;Parent=MS.gene062424.t1
chr7.3	CDS	75239643	75239729	75239643	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75239821	75239875	75239821	ID=MS.gene062424.t1.exon5;Parent=MS.gene062424.t1
chr7.3	CDS	75239821	75239875	75239821	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75239955	75240026	75239955	ID=MS.gene062424.t1.exon6;Parent=MS.gene062424.t1
chr7.3	CDS	75239955	75240026	75239955	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75240329	75240391	75240329	ID=MS.gene062424.t1.exon7;Parent=MS.gene062424.t1
chr7.3	CDS	75240329	75240391	75240329	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75240787	75240901	75240787	ID=MS.gene062424.t1.exon8;Parent=MS.gene062424.t1
chr7.3	CDS	75240787	75240901	75240787	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75240973	75241083	75240973	ID=MS.gene062424.t1.exon9;Parent=MS.gene062424.t1
chr7.3	CDS	75240973	75241083	75240973	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75241442	75241513	75241442	ID=MS.gene062424.t1.exon10;Parent=MS.gene062424.t1
chr7.3	CDS	75241442	75241513	75241442	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75241711	75241776	75241711	ID=MS.gene062424.t1.exon11;Parent=MS.gene062424.t1
chr7.3	CDS	75241711	75241776	75241711	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1
chr7.3	exon	75241853	75241954	75241853	ID=MS.gene062424.t1.exon12;Parent=MS.gene062424.t1
chr7.3	CDS	75241853	75241954	75241853	ID=cds.MS.gene062424.t1;Parent=MS.gene062424.t1

Gene Sequence

>MS.gene062424

ATGATGTCTAGAGATGATTCTAGAAGATTTTCTGCAGATCAAGCTTTAGGTATGATATAACAAACTGTTAATTTCAGATTTTGATTAATTAAATTGATTATGAATTAGTATTTGATTATTGATTTTAATTAACTTTATGAATTAGTAGTGAAAATACTAATGTTGATTTTAATTAACTAATGATGCTAGTTTTAAATACTAATGTTGATCCTTTACTTGTTGAACCTACCGATCTTTAGGTTAAGAAGGTTAAGCCACACGTGCTCCTAGGTTTGTCTGGTGTTGGTGGTATTTTCAACAAGGAGGTAATTACCTTCTACACTTGCATTTCTATCATGTCATATTGAAAATATATTAAATAATTAAATTTAATAAATATCTTTGACAGCTTCGGTTACTGTATGAACTTTTTTTTAGTTGAGTTCTTAAAATTTAATAGTTGAGATTCAGTTCAGGTGCTTAAGGCAATGAGAGAATCCGTTTCAACAAAGCCTGCTATCTTTGCCATGTCTAATCCCACCTTGAATGGTTTGTTTGTACAAAGCTTATTAAGCATTCACATTTCCATAGTAACATTTACACTTTTTTTAACTCATTTTTGTTTGTCTATTACGGGGGACAGCCGAGTGCACTGCTATTGATGCTTTTAATCATGTTGGGGAACATATGGTGTTTGCCAGTGGAAGCCCTTTCGAAAATGTAGATTTTGGTAATTACCTCTTTCATTAGTTTTTATTATATCGTGTACAGTTCACATTTTCAATAGATAATTGTTTTTGTGCAATTAAAATTTTGTGTAGGTGATGGGAAAGTAGGCTATGTAAATCAAGCAAACAATATGTACCTTTTCCCAGGGTATGGCATGCTTTTTTGGATTTTATTTACTTACAATTGAGTTATTGGTGTAACTTTTATTTTGAACTTTACATTTCAGAATTGGGTTGGGAACACTTCTGTCAGGTGCTCATCTTATTACAGATGGAATGTTGCAGGCAGCTGCTGAATGGTATGATGTTAATTTAGACAATTGTTTATTTTTTACTATATTGTGAGAGTATTTTTTACTATACTGTGAGAGTATTTTTTACTGCTCAAATTGTCAACATGATAACTCTATACACGACATGCACTCACCATAAGTTTTACAGCGCTTCTTATCATTCATGCTTTTCTTAAGCAAAGCATAAATGATCACCGTTTTGTTAATAATTTCATTGGATTAAATTGCAAATCCTACTTTATTTTTATATGTCACCTGTTGCATCTTGTCATATACATTGCTAACATTATGCATTTTCATTTTAACAGCCTTGCTTCATACATGGCCGAGGAAGATATTTTACAAGGAATTCTCTATCCATCTGTTAATAGGTAATGTTCCCTTTTCCCTCTGATTATTTTTTTTGTTTGTTCTGCTTTTCCAGTCTCTTTTATGACATGAACAGAACAAATGAAGTTAAAATTTTTTAATGAAACTAATGTGGTTTTCTGTATGTTATCTAGGATTTAGAAACCAAATATGATACCATATATCTAACAAAATATGTGTTCGCCCACTATCTCTTGTATAGTCGTTTACCAAATGTAAGAGCTTCGGATATGGTTAAAAAAACGTGTTCTCGACAACATTCTTATTAGAATAGAAAGTCTTGTTGATTTGGTTGTTTGTTGAGAAGCTAAAAAGTTCACAGTAGTTGAAATTATGGCCTGTTCTCAGTAGTTGAAATCTTGGTCTTCTCTTATTAACCCTACTTTGCGTACTGCAGCATACGAGATGTTACCGCAGAGGTTGGAGCTGCAGTGCTTCGAGCAGCAGTTGAAGAAGATCTGGCAGAGGGACATGGTGATGTCTGGCCTAGAGAACTTGCAAACATGTCAAAAGTATGCAACTTTTCCCAATTTATCAAAAGGATAGAGCCACATCTATGTCTCTTGAAGTCAGTACCTAATAGAGCACCTTTTATCTACACATGCTATGCCTTGGTGCATGGTCAGACCATGGTGAAAGGACTGGCGATTTCTACGCTTGCAATCGTTATGAAGCAGCTAAACAAGAGGGGGTGGTAAGAGACTTAGAAATACTCAACTTCTCTTGATTCAGTTTTCAGTATACTATTAAACCACATTCCCGAGACATATTTTTATAAAGATATCAGACTGTGAAAGAAATCTTGTTGTATGTAGTATGATGAAACTGAAAAAAGAAGAGAAATGGCAAAGAATTCATTAGAGAGGTACACACATTATTATGAGAAATCTTGTTTATTTTTTGATTATTTTTTGTTGGGTATTTGTTAATTTTGTTTCATGGTCTTTTGATTATTTTTTGTTGGGTATTTGTTAATTTTAGATGCTTTCAGATCAAAGACTTACAGTAAATCTTAATCTGTGTTCTTATGGTGTCCTTTTTTTTATTGGCAGATGGAGAAGCTTAGTGACACACAGTGCCAACCTGAGTCACAACTTAAGTTCATAACCGAGGCTTGGTTACAGGTAAATGAATGAATATTTGGTTATACCTTTGTTCAAAGTTTTTGAAATCGCGTCATTCAAAGGATTCATATCGAATGCGTTGAATGAACTGATGCAATGTGGAGTGAAAAAAACATTTGATTTTTAATAGGACAATCTATTCTTATGCATTGCTGACAGAAACTTGGAATATTAGATTATATTCTTATGCATTGCAGACAGAAACTTGGAATATTAGACGTGAAAAGTACACAACCACACACTGGTATCTCCTTGATACCAAGGTATCTCTCATATTTATTGTCTAACTGAGTTTTGGTGTTGTTAAAGTTTATACGGGGTTGATTTTTTTATTTTCAGAAAACAATGGAGCAAATGGTGGATAAGCGACTTAAAAACTGTGTTAGATGGTTAAGGGGGTTGAAGAAGAGTACATTAAAGAAAAATAAAAGTTTCAGATAG

Protein sequence

>MS.gene062424.t1

MMSRDDSRRFSADQALGLSGVGGIFNKEVLKAMRESVSTKPAIFAMSNPTLNAECTAIDAFNHVGEHMVFASGSPFENVDFGDGKVGYVNQANNMYLFPGIGLGTLLSGAHLITDGMLQAAAECLASYMAEEDILQGILYPSVNSIRDVTAEVGAAVLRAAVEEDLAEGHGDVWPRELANMSKSTFYLHMLCLGAWSDHGERTGDFYACNRYEAAKQEGVMEKLSDTQCQPESQLKFITEAWLQTETWNIRREKYTTTHWYLLDTKKTMEQMVDKRLKNCVRWLRGLKKSTLKKNKSFR