AlfalfaGEDB | MS.gene062700

M. sativa cultivar XinJiangDaYe / MS.gene062700

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062700.t1	XP_013466070.1	100	188	0	0	1	188	1	188	5.20E-103	383.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062700.t1	O49929	86.7	188	25	0	1	188	1	188	7.8e-92	337.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062700.t1	A0A072VDK2	100.0	188	0	0	1	188	1	188	3.7e-103	383.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054638	MS.gene062700	0.830773	2.43E-55	-1.69E-46
MS.gene057684	MS.gene062700	0.806731	7.13E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062700.t1	MTR_1g022050	100.000	188	0	0	1	188	1	188	1.08e-139	388

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062700.t1	AT1G45170	69.149	188	57	1	1	188	2	188	2.87e-96	278
MS.gene062700.t1	AT5G42960	67.021	188	60	2	1	188	3	188	1.17e-93	272
MS.gene062700.t1	AT1G45170	53.191	188	41	2	1	188	2	142	3.03e-62	191

Find 52 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCGAATTATGGTGTTGAATC+TGG	0.218241	1.4:+2347118	MS.gene062700:CDS
AGGGGATAACAGAACAATTT+TGG	0.234866	1.4:+2347057	MS.gene062700:CDS
AATAAGGTTTCAGCGAATTA+TGG	0.297969	1.4:+2347106	MS.gene062700:CDS
CAATTGTAGTCAAACCCTTA+TGG	0.311402	1.4:-2347168	None:intergenic
TTCAATTTATGAACACAGTT+AGG	0.317886	1.4:+2346993	MS.gene062700:CDS
GGCGACGGAGGCTGCGGCGG+CGG	0.338930	1.4:-2346736	None:intergenic
AACAATGAAAGCCGCTGTTA+AGG	0.348424	1.4:+2346689	None:intergenic
GCTGTTTCGAGGAAAGTTTA+TGG	0.364076	1.4:+2347229	MS.gene062700:CDS
ACAATGAAAGCCGCTGTTAA+GGG	0.378379	1.4:+2346690	None:intergenic
TGTGTGGGACCCATCGAATA+AGG	0.383360	1.4:+2347090	MS.gene062700:CDS
CGACTACAACGTCCCCAAAA+AGG	0.387317	1.4:+2346872	MS.gene062700:CDS
CAGACCACGAGTAAGAAGTT+AGG	0.393154	1.4:+2347277	MS.gene062700:CDS
ATTAACGGCCCAAGCTTAAC+CGG	0.393393	1.4:+2346810	MS.gene062700:CDS
ACAGACGCTACTTTCATTAA+CGG	0.400228	1.4:+2346795	MS.gene062700:CDS
GCCTCCGTCGCCTTCAACGC+CGG	0.402791	1.4:+2346747	MS.gene062700:CDS
TTTGACATCGCCGGCGTTGA+AGG	0.416806	1.4:-2346757	None:intergenic
TAACCTACATACACAGCAAA+GGG	0.443140	1.4:+2347038	MS.gene062700:CDS
GAAGTATAGCTATGTCCATA+AGG	0.446086	1.4:+2347153	MS.gene062700:CDS
TCAATTTATGAACACAGTTA+GGG	0.463428	1.4:+2346994	MS.gene062700:CDS
AGGACTAAGCCGGTTAAGCT+TGG	0.467012	1.4:-2346819	None:intergenic
GTAGTCGATACTGAAGGAAC+CGG	0.468560	1.4:-2346856	None:intergenic
AAGTATAGCTATGTCCATAA+GGG	0.470434	1.4:+2347154	MS.gene062700:CDS
GGACTAAGCCGGTTAAGCTT+GGG	0.473049	1.4:-2346818	None:intergenic
CGAATTATGGTGTTGAATCT+GGG	0.476174	1.4:+2347119	MS.gene062700:CDS
AGTTAGGGTTTGAGTGGTCG+AGG	0.482159	1.4:+2347293	MS.gene062700:CDS
ATCCCCTTTGCTGTGTATGT+AGG	0.496851	1.4:-2347041	None:intergenic
AGACCACGAGTAAGAAGTTA+GGG	0.497058	1.4:+2347278	MS.gene062700:CDS
TTCGCTGAAACCTTATTCGA+TGG	0.499631	1.4:-2347100	None:intergenic
GTTGAAGGCGACGGAGGCTG+CGG	0.500163	1.4:-2346742	None:intergenic
GTCGAGGAATTCGAAACAAA+GGG	0.505059	1.4:+2347309	MS.gene062700:CDS
TTCTACGGCGAGGACTAAGC+CGG	0.514419	1.4:-2346829	None:intergenic
TTATGGTGATGACTCATTGA+AGG	0.523909	1.4:+2347246	MS.gene062700:CDS
GAAGGCGACGGAGGCTGCGG+CGG	0.533980	1.4:-2346739	None:intergenic
TGCTCGGAGTTTGACATCGC+CGG	0.539580	1.4:-2346766	None:intergenic
TGTATGTAGGTTAAATTCAA+CGG	0.544412	1.4:-2347028	None:intergenic
TCGCTGAAACCTTATTCGAT+GGG	0.554144	1.4:-2347099	None:intergenic
TTCTTAGCAACATCATAACT+AGG	0.560239	1.4:-2347193	None:intergenic
TTTGGACGGAACATTTGTGT+GGG	0.592902	1.4:+2347075	MS.gene062700:CDS
ATCGCCGGCGTTGAAGGCGA+CGG	0.596432	1.4:-2346751	None:intergenic
GTCGTATTTGCCCTTAACAG+CGG	0.599655	1.4:-2346700	None:intergenic
TAGCGTCTGTTATCGATGCT+CGG	0.602349	1.4:-2346782	None:intergenic
GGTCGAGGAATTCGAAACAA+AGG	0.607936	1.4:+2347308	MS.gene062700:CDS
TAGTCGATACTGAAGGAACC+GGG	0.608857	1.4:-2346855	None:intergenic
TTAACCTACATACACAGCAA+AGG	0.618005	1.4:+2347037	MS.gene062700:CDS
GCCGGCGTTGAAGGCGACGG+AGG	0.618807	1.4:-2346748	None:intergenic
GTAAGAAGTTAGGGTTTGAG+TGG	0.628064	1.4:+2347287	MS.gene062700:CDS
TGATGTTGCTAAGAATGCGT+GGG	0.641670	1.4:+2347201	MS.gene062700:CDS
AAACCCTAACTTCTTACTCG+TGG	0.657874	1.4:-2347281	None:intergenic
ATGATGTTGCTAAGAATGCG+TGG	0.662892	1.4:+2347200	MS.gene062700:CDS
GACGTTGTAGTCGATACTGA+AGG	0.665017	1.4:-2346862	None:intergenic
AACCTACATACACAGCAAAG+GGG	0.729150	1.4:+2347039	MS.gene062700:CDS
TCGAGGAATTCGAAACAAAG+GGG	0.745078	1.4:+2347310	MS.gene062700:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAAATAAATAAATTGAAC+CGG	-	chr1.4:2346935-2346954	None:intergenic	10.0%
!!!	TTTTTTTTGTTTACCTTTTT+GGG	-	chr1.4:2346888-2346907	None:intergenic	15.0%
!!!	TTTTTTTTTGTTTACCTTTT+TGG	-	chr1.4:2346889-2346908	None:intergenic	15.0%
!!!	TTTTTTTGTTTACCTTTTTG+GGG	-	chr1.4:2346887-2346906	None:intergenic	20.0%
!	ATAAATAAATTGAACCGGTT+TGG	-	chr1.4:2346930-2346949	None:intergenic	25.0%
!	TCAATTTATGAACACAGTTA+GGG	+	chr1.4:2346994-2347013	MS.gene062700:CDS	25.0%
!	TGTATGTAGGTTAAATTCAA+CGG	-	chr1.4:2347031-2347050	None:intergenic	25.0%
!	TTCAATTTATGAACACAGTT+AGG	+	chr1.4:2346993-2347012	MS.gene062700:CDS	25.0%
!!!	TTTTTGTTTAATTTTGGTGC+AGG	+	chr1.4:2346961-2346980	MS.gene062700:intron	25.0%
	AAGTATAGCTATGTCCATAA+GGG	+	chr1.4:2347154-2347173	MS.gene062700:CDS	30.0%
	AATAAGGTTTCAGCGAATTA+TGG	+	chr1.4:2347106-2347125	MS.gene062700:CDS	30.0%
	TTCTTAGCAACATCATAACT+AGG	-	chr1.4:2347196-2347215	None:intergenic	30.0%
!	ATCCATTTTCTAAACCAAAC+CGG	+	chr1.4:2346913-2346932	MS.gene062700:intron	30.0%
!	GATAACAGAACAATTTTGGA+CGG	+	chr1.4:2347061-2347080	MS.gene062700:CDS	30.0%
!!	TAATTTTGGTGCAGGATTTT+AGG	+	chr1.4:2346969-2346988	MS.gene062700:intron	30.0%
	CAATTGTAGTCAAACCCTTA+TGG	-	chr1.4:2347171-2347190	None:intergenic	35.0%
	GAAGTATAGCTATGTCCATA+AGG	+	chr1.4:2347153-2347172	MS.gene062700:CDS	35.0%
	TAACCTACATACACAGCAAA+GGG	+	chr1.4:2347038-2347057	MS.gene062700:CDS	35.0%
	TTAACCTACATACACAGCAA+AGG	+	chr1.4:2347037-2347056	MS.gene062700:CDS	35.0%
!	ACAGACGCTACTTTCATTAA+CGG	+	chr1.4:2346795-2346814	MS.gene062700:CDS	35.0%
!	AGGGGATAACAGAACAATTT+TGG	+	chr1.4:2347057-2347076	MS.gene062700:CDS	35.0%
!	CGAATTATGGTGTTGAATCT+GGG	+	chr1.4:2347119-2347138	MS.gene062700:CDS	35.0%
!	TTATGGTGATGACTCATTGA+AGG	+	chr1.4:2347246-2347265	MS.gene062700:CDS	35.0%
!!	AACCGGTTTGGTTTAGAAAA+TGG	-	chr1.4:2346918-2346937	None:intergenic	35.0%
!!	GAAACAAAGGGGTTGTTTTA+AGG	+	chr1.4:2347321-2347340	MS.gene062700:CDS	35.0%
	AAACCCTAACTTCTTACTCG+TGG	-	chr1.4:2347284-2347303	None:intergenic	40.0%
	AACCTACATACACAGCAAAG+GGG	+	chr1.4:2347039-2347058	MS.gene062700:CDS	40.0%
	AGACCACGAGTAAGAAGTTA+GGG	+	chr1.4:2347278-2347297	MS.gene062700:CDS	40.0%
	ATGATGTTGCTAAGAATGCG+TGG	+	chr1.4:2347200-2347219	MS.gene062700:CDS	40.0%
	GTAAGAAGTTAGGGTTTGAG+TGG	+	chr1.4:2347287-2347306	MS.gene062700:CDS	40.0%
	GTCGAGGAATTCGAAACAAA+GGG	+	chr1.4:2347309-2347328	MS.gene062700:CDS	40.0%
	TCGAGGAATTCGAAACAAAG+GGG	+	chr1.4:2347310-2347329	MS.gene062700:CDS	40.0%
	TCGCTGAAACCTTATTCGAT+GGG	-	chr1.4:2347102-2347121	None:intergenic	40.0%
	TGATGTTGCTAAGAATGCGT+GGG	+	chr1.4:2347201-2347220	MS.gene062700:CDS	40.0%
	TTCGCTGAAACCTTATTCGA+TGG	-	chr1.4:2347103-2347122	None:intergenic	40.0%
	TTTGGACGGAACATTTGTGT+GGG	+	chr1.4:2347075-2347094	MS.gene062700:CDS	40.0%
!	GCGAATTATGGTGTTGAATC+TGG	+	chr1.4:2347118-2347137	MS.gene062700:CDS	40.0%
!	GCTGTTTCGAGGAAAGTTTA+TGG	+	chr1.4:2347229-2347248	MS.gene062700:CDS	40.0%
!	TTTTGGACGGAACATTTGTG+TGG	+	chr1.4:2347074-2347093	MS.gene062700:CDS	40.0%
	ATTAACGGCCCAAGCTTAAC+CGG	+	chr1.4:2346810-2346829	MS.gene062700:CDS	45.0%
	CAGACCACGAGTAAGAAGTT+AGG	+	chr1.4:2347277-2347296	MS.gene062700:CDS	45.0%
	GACGTTGTAGTCGATACTGA+AGG	-	chr1.4:2346865-2346884	None:intergenic	45.0%
	GGTCGAGGAATTCGAAACAA+AGG	+	chr1.4:2347308-2347327	MS.gene062700:CDS	45.0%
	GTAGTCGATACTGAAGGAAC+CGG	-	chr1.4:2346859-2346878	None:intergenic	45.0%
	GTCGTATTTGCCCTTAACAG+CGG	-	chr1.4:2346703-2346722	None:intergenic	45.0%
	TAGCGTCTGTTATCGATGCT+CGG	-	chr1.4:2346785-2346804	None:intergenic	45.0%
	TAGTCGATACTGAAGGAACC+GGG	-	chr1.4:2346858-2346877	None:intergenic	45.0%
!	ATCCCCTTTGCTGTGTATGT+AGG	-	chr1.4:2347044-2347063	None:intergenic	45.0%
!	GAAGGAACCGGGTTTTTCTA+CGG	-	chr1.4:2346847-2346866	None:intergenic	45.0%
!!!	TATTTATTTTTGTTTAATTT+TGG	+	chr1.4:2346955-2346974	MS.gene062700:intron	5.0%
	AGGACTAAGCCGGTTAAGCT+TGG	-	chr1.4:2346822-2346841	None:intergenic	50.0%
	AGTTAGGGTTTGAGTGGTCG+AGG	+	chr1.4:2347293-2347312	MS.gene062700:CDS	50.0%
	CGACTACAACGTCCCCAAAA+AGG	+	chr1.4:2346872-2346891	MS.gene062700:CDS	50.0%
	GGACTAAGCCGGTTAAGCTT+GGG	-	chr1.4:2346821-2346840	None:intergenic	50.0%
	TGTGTGGGACCCATCGAATA+AGG	+	chr1.4:2347090-2347109	MS.gene062700:CDS	50.0%
!!!	CGTGGGATTTTGCTGTTTCG+AGG	+	chr1.4:2347218-2347237	MS.gene062700:CDS	50.0%
	GTCCTCGCCGTAGAAAAACC+CGG	+	chr1.4:2346837-2346856	MS.gene062700:CDS	55.0%
	TGCTCGGAGTTTGACATCGC+CGG	-	chr1.4:2346769-2346788	None:intergenic	55.0%
	TTCTACGGCGAGGACTAAGC+CGG	-	chr1.4:2346832-2346851	None:intergenic	55.0%
!	AACCGGGTTTTTCTACGGCG+AGG	-	chr1.4:2346842-2346861	None:intergenic	55.0%
!	TTTGACATCGCCGGCGTTGA+AGG	-	chr1.4:2346760-2346779	None:intergenic	55.0%
	GTTGAAGGCGACGGAGGCTG+CGG	-	chr1.4:2346745-2346764	None:intergenic	65.0%
!	ATCGCCGGCGTTGAAGGCGA+CGG	-	chr1.4:2346754-2346773	None:intergenic	65.0%
	GCCTCCGTCGCCTTCAACGC+CGG	+	chr1.4:2346747-2346766	MS.gene062700:CDS	70.0%
	GAAGGCGACGGAGGCTGCGG+CGG	-	chr1.4:2346742-2346761	None:intergenic	75.0%
!	GCCGGCGTTGAAGGCGACGG+AGG	-	chr1.4:2346751-2346770	None:intergenic	75.0%
!	GGCGACGGAGGCTGCGGCGG+CGG	-	chr1.4:2346739-2346758	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	2346693	2347354	2346693	ID=MS.gene062700
chr1.4	mRNA	2346693	2347354	2346693	ID=MS.gene062700.t1;Parent=MS.gene062700
chr1.4	exon	2346693	2346893	2346693	ID=MS.gene062700.t1.exon1;Parent=MS.gene062700.t1
chr1.4	CDS	2346693	2346893	2346693	ID=cds.MS.gene062700.t1;Parent=MS.gene062700.t1
chr1.4	exon	2346983	2347354	2346983	ID=MS.gene062700.t1.exon2;Parent=MS.gene062700.t1
chr1.4	CDS	2346983	2347354	2346983	ID=cds.MS.gene062700.t1;Parent=MS.gene062700.t1

Gene Sequence

>MS.gene062700

ATGAAAGCCGCTGTTAAGGGCAAATACGACAATGACAAAAACGCCGCCGCCGCAGCCTCCGTCGCCTTCAACGCCGGCGATGTCAAACTCCGAGCATCGATAACAGACGCTACTTTCATTAACGGCCCAAGCTTAACCGGCTTAGTCCTCGCCGTAGAAAAACCCGGTTCCTTCAGTATCGACTACAACGTCCCCAAAAAGGTAAACAAAAAAAAATTCAATCCATTTTCTAAACCAAACCGGTTCAATTTATTTATTTATTTATTTATTTTTGTTTAATTTTGGTGCAGGATTTTAGGTTTCAATTTATGAACACAGTTAGGGTTGCTGATAAACCGTTGAATTTAACCTACATACACAGCAAAGGGGATAACAGAACAATTTTGGACGGAACATTTGTGTGGGACCCATCGAATAAGGTTTCAGCGAATTATGGTGTTGAATCTGGGAATTGTAGATTGAAGTATAGCTATGTCCATAAGGGTTTGACTACAATTGAACCTAGTTATGATGTTGCTAAGAATGCGTGGGATTTTGCTGTTTCGAGGAAAGTTTATGGTGATGACTCATTGAAGGCGACGTATCAGACCACGAGTAAGAAGTTAGGGTTTGAGTGGTCGAGGAATTCGAAACAAAGGGGTTGTTTTAAGGTTAGTTGTTGA

Protein sequence

>MS.gene062700.t1

MKAAVKGKYDNDKNAAAAASVAFNAGDVKLRASITDATFINGPSLTGLVLAVEKPGSFSIDYNVPKKDFRFQFMNTVRVADKPLNLTYIHSKGDNRTILDGTFVWDPSNKVSANYGVESGNCRLKYSYVHKGLTTIEPSYDVAKNAWDFAVSRKVYGDDSLKATYQTTSKKLGFEWSRNSKQRGCFKVSC