AlfalfaGEDB | MS.gene06310

M. sativa cultivar XinJiangDaYe / MS.gene06310

KEGG: K17362
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06310.t1	RHN70040.1	82.4	68	12	0	1	68	1	68	3.60E-23	117.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06310.t1	G7J2P5	82.4	68	12	0	1	68	1	68	2.6e-23	117.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049194	MS.gene06310	0.802017	6.85E-49	-1.69E-46
MS.gene053074	MS.gene06310	0.811904	5.55E-51	-1.69E-46
MS.gene055359	MS.gene06310	0.801986	6.95E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06310.t1	MTR_3g098180	82.353	68	12	0	1	68	1	68	2.17e-35	118
MS.gene06310.t1	MTR_3g098160	65.753	73	17	2	1	68	1	70	9.65e-25	90.5
MS.gene06310.t1	MTR_3g098160	65.753	73	17	2	1	68	1	70	2.59e-24	90.5
MS.gene06310.t1	MTR_3g098170	74.576	59	12	1	4	62	9	64	5.22e-23	86.3
MS.gene06310.t1	MTR_3g098170	74.576	59	12	1	4	62	9	64	6.95e-23	86.7
MS.gene06310.t1	MTR_3g098130	69.492	59	18	0	4	62	9	67	1.48e-22	85.9
MS.gene06310.t1	MTR_3g098130	69.492	59	18	0	4	62	47	105	2.66e-22	86.3
MS.gene06310.t1	MTR_3g098120	58.209	67	26	1	2	68	48	112	1.23e-19	79.3
MS.gene06310.t1	MTR_3g098140	70.370	54	15	1	15	68	9	61	5.11e-18	74.3
MS.gene06310.t1	MTR_3g098100	59.322	59	21	2	5	62	2	58	1.65e-14	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06310.t1	AT1G04290	54.237	59	26	1	5	62	2	60	3.82e-13	61.6
MS.gene06310.t1	AT1G04290	49.231	65	32	1	5	68	2	66	5.22e-13	60.5

Find 33 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAATTTCCTCCGAAGTTTC+TGG	0.138608	3.1:-77860028	MS.gene06310:CDS
TGAATTAACTTGCAGAATTC+AGG	0.239073	3.1:-77859819	MS.gene06310:intron
GTTTCTGGAACATCTAATCT+TGG	0.344011	3.1:-77860013	MS.gene06310:CDS
GAAAGGAGAAGCGTCGTTAA+AGG	0.351047	3.1:-77860061	MS.gene06310:CDS
GCTGGATTTCAGTTGGATAC+TGG	0.394930	3.1:-77859729	MS.gene06310:CDS
AAGAGGTACTTGGAGGAGAA+AGG	0.429137	3.1:-77860078	MS.gene06310:CDS
CGCTTGGATCTCATTGAGCC+TGG	0.445136	3.1:-77859982	MS.gene06310:CDS
AATCTTGGGTGGCTTACGCT+TGG	0.453352	3.1:-77859998	MS.gene06310:CDS
TCAATCGCAATTCCCGCCGC+TGG	0.459843	3.1:-77859747	MS.gene06310:CDS
TTTCTGGAACATCTAATCTT+GGG	0.471271	3.1:-77860012	MS.gene06310:CDS
GGAATCAGTGAAGAGGTACT+TGG	0.484190	3.1:-77860088	MS.gene06310:CDS
TCCCGCCGCTGGATTTCAGT+TGG	0.485139	3.1:-77859736	MS.gene06310:CDS
CATCATTACTCTGGTGGATG+TGG	0.494972	3.1:-77859775	MS.gene06310:CDS
CACATCCACCAGAGTAATGA+TGG	0.509226	3.1:+77859776	None:intergenic
CTGGAACATCTAATCTTGGG+TGG	0.511525	3.1:-77860009	MS.gene06310:CDS
ATTGAGAAAACGACACGGCC+AGG	0.517820	3.1:+77859964	None:intergenic
TCCAACTGAAATCCAGCGGC+GGG	0.545347	3.1:+77859735	None:intergenic
ATTAGATGTTCCAGAAACTT+CGG	0.547234	3.1:+77860018	None:intergenic
TGGTGCCATCATTACTCTGG+TGG	0.548725	3.1:-77859781	MS.gene06310:CDS
CCTTGGTGCCATCATTACTC+TGG	0.553351	3.1:-77859784	MS.gene06310:CDS
ATTACTCTGGTGGATGTGGT+AGG	0.556926	3.1:-77859771	MS.gene06310:CDS
ATCAGTGAAGAGGTACTTGG+AGG	0.578355	3.1:-77860085	MS.gene06310:CDS
TCAGGTACGTACTTGCACCT+TGG	0.609189	3.1:-77859801	MS.gene06310:CDS
CAGATTTGGAATCAGTGAAG+AGG	0.627422	3.1:-77860095	MS.gene06310:CDS
ATCCAACTGAAATCCAGCGG+CGG	0.646543	3.1:+77859734	None:intergenic
AGATGTTCCAGAAACTTCGG+AGG	0.661872	3.1:+77860021	None:intergenic
AGAGTACGAACGAGTAAACG+TGG	0.662463	3.1:+77859931	None:intergenic
CCAGAGTAATGATGGCACCA+AGG	0.663656	3.1:+77859784	None:intergenic
GAGTACGAACGAGTAAACGT+GGG	0.664966	3.1:+77859932	None:intergenic
AGTACGAACGAGTAAACGTG+GGG	0.701776	3.1:+77859933	None:intergenic
AGTATCCAACTGAAATCCAG+CGG	0.715519	3.1:+77859731	None:intergenic
TGTTCATTGAGAAAACGACA+CGG	0.782322	3.1:+77859959	None:intergenic
GTACGAACGAGTAAACGTGG+GGG	0.814375	3.1:+77859934	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATTAGATGTTCCAGAAACTT+CGG	+	chr3.1:77859807-77859826	None:intergenic	30.0%
!!	TGAATTAACTTGCAGAATTC+AGG	-	chr3.1:77860003-77860022	MS.gene06310:CDS	30.0%
!!	TTTCTGGAACATCTAATCTT+GGG	-	chr3.1:77859810-77859829	MS.gene06310:intron	30.0%
	TAAATTTCCTCCGAAGTTTC+TGG	-	chr3.1:77859794-77859813	MS.gene06310:CDS	35.0%
	TGTTCATTGAGAAAACGACA+CGG	+	chr3.1:77859866-77859885	None:intergenic	35.0%
!!	GTTTCTGGAACATCTAATCT+TGG	-	chr3.1:77859809-77859828	MS.gene06310:intron	35.0%
	AGTATCCAACTGAAATCCAG+CGG	+	chr3.1:77860094-77860113	None:intergenic	40.0%
!	CAGATTTGGAATCAGTGAAG+AGG	-	chr3.1:77859727-77859746	MS.gene06310:CDS	40.0%
	AGAGTACGAACGAGTAAACG+TGG	+	chr3.1:77859894-77859913	None:intergenic	45.0%
	AGATGTTCCAGAAACTTCGG+AGG	+	chr3.1:77859804-77859823	None:intergenic	45.0%
	AGTACGAACGAGTAAACGTG+GGG	+	chr3.1:77859892-77859911	None:intergenic	45.0%
	CACATCCACCAGAGTAATGA+TGG	+	chr3.1:77860049-77860068	None:intergenic	45.0%
	CTGGAACATCTAATCTTGGG+TGG	-	chr3.1:77859813-77859832	MS.gene06310:intron	45.0%
	GAGTACGAACGAGTAAACGT+GGG	+	chr3.1:77859893-77859912	None:intergenic	45.0%
	GCTGGATTTCAGTTGGATAC+TGG	-	chr3.1:77860093-77860112	MS.gene06310:CDS	45.0%
!	ATTACTCTGGTGGATGTGGT+AGG	-	chr3.1:77860051-77860070	MS.gene06310:CDS	45.0%
!	CATCATTACTCTGGTGGATG+TGG	-	chr3.1:77860047-77860066	MS.gene06310:CDS	45.0%
!!	AAGAGGTACTTGGAGGAGAA+AGG	-	chr3.1:77859744-77859763	MS.gene06310:CDS	45.0%
!!	ATCAGTGAAGAGGTACTTGG+AGG	-	chr3.1:77859737-77859756	MS.gene06310:CDS	45.0%
!!	GAAAGGAGAAGCGTCGTTAA+AGG	-	chr3.1:77859761-77859780	MS.gene06310:CDS	45.0%
!!	GGAATCAGTGAAGAGGTACT+TGG	-	chr3.1:77859734-77859753	MS.gene06310:CDS	45.0%
	AATCTTGGGTGGCTTACGCT+TGG	-	chr3.1:77859824-77859843	MS.gene06310:intron	50.0%
	ATCCAACTGAAATCCAGCGG+CGG	+	chr3.1:77860091-77860110	None:intergenic	50.0%
	ATTGAGAAAACGACACGGCC+AGG	+	chr3.1:77859861-77859880	None:intergenic	50.0%
	CCAGAGTAATGATGGCACCA+AGG	+	chr3.1:77860041-77860060	None:intergenic	50.0%
	GTACGAACGAGTAAACGTGG+GGG	+	chr3.1:77859891-77859910	None:intergenic	50.0%
	TCAGGTACGTACTTGCACCT+TGG	-	chr3.1:77860021-77860040	MS.gene06310:CDS	50.0%
!!	CCTTGGTGCCATCATTACTC+TGG	-	chr3.1:77860038-77860057	MS.gene06310:CDS	50.0%
!!	TGGTGCCATCATTACTCTGG+TGG	-	chr3.1:77860041-77860060	MS.gene06310:CDS	50.0%
	CGCTTGGATCTCATTGAGCC+TGG	-	chr3.1:77859840-77859859	MS.gene06310:intron	55.0%
	TCCAACTGAAATCCAGCGGC+GGG	+	chr3.1:77860090-77860109	None:intergenic	55.0%
	TCAATCGCAATTCCCGCCGC+TGG	-	chr3.1:77860075-77860094	MS.gene06310:CDS	60.0%
	TCCCGCCGCTGGATTTCAGT+TGG	-	chr3.1:77860086-77860105	MS.gene06310:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	77859717	77860127	77859717	ID=MS.gene06310
chr3.1	mRNA	77859717	77860127	77859717	ID=MS.gene06310.t1;Parent=MS.gene06310
chr3.1	exon	77859938	77860127	77859938	ID=MS.gene06310.t1.exon1;Parent=MS.gene06310.t1
chr3.1	CDS	77859938	77860127	77859938	ID=cds.MS.gene06310.t1;Parent=MS.gene06310.t1
chr3.1	exon	77859717	77859826	77859717	ID=MS.gene06310.t1.exon2;Parent=MS.gene06310.t1
chr3.1	CDS	77859717	77859826	77859717	ID=cds.MS.gene06310.t1;Parent=MS.gene06310.t1

Gene Sequence

>MS.gene06310

ATGGAAAAAACAGATTTGGAATCAGTGAAGAGGTACTTGGAGGAGAAAGGAGAAGCGTCGTTAAAGGTAGATGATGATAAATTTCCTCCGAAGTTTCTGGAACATCTAATCTTGGGTGGCTTACGCTTGGATCTCATTGAGCCTGGCCGTGTCGTTTTCTCAATGAACATACCCCCACGTTTACTCGTTCGTACTCTCTTCAATCTTCTTATTCTTCTTCTTCTTCTTCTTCATCATCATATTTTTTATGATGAATTTTGTTGTTGTTTTGAGCTGAATTTATTAATGAATTAACTTGCAGAATTCAGGTACGTACTTGCACCTTGGTGCCATCATTACTCTGGTGGATGTGGTAGGTTCAATCGCAATTCCCGCCGCTGGATTTCAGTTGGATACTGGTACTTCCGTTGA

Protein sequence

>MS.gene06310.t1

MEKTDLESVKRYLEEKGEASLKVDDDKFPPKFLEHLILGGLRLDLIEPGRVVFSMNIPPRLLVQFRYVLAPWCHHYSGGCGRFNRNSRRWISVGYWYFR