Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063364.t1 | AES66446.1 | 100 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 6.40E-30 | 139.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063364.t1 | G7IH42 | 100.0 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 4.6e-30 | 139.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049267 | MS.gene063364 | 0.84395 | 1.02E-58 | -1.69E-46 |
| MS.gene058840 | MS.gene063364 | 0.806734 | 7.12E-50 | -1.69E-46 |
| MS.gene060578 | MS.gene063364 | 0.801726 | 7.86E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063364.t1 | MTR_2g069400 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 7.62e-49 | 148 |
| MS.gene063364.t1 | MTR_2g069430 | 60.526 | 76 | 26 | 1 | 1 | 72 | 1 | 76 | 2.08e-18 | 71.6 |
| MS.gene063364.t1 | MTR_2g069420 | 58.228 | 79 | 25 | 3 | 1 | 72 | 1 | 78 | 2.85e-15 | 63.9 |
| MS.gene063364.t1 | MTR_4g091090 | 61.842 | 76 | 22 | 3 | 4 | 72 | 5 | 80 | 2.99e-15 | 63.9 |
| MS.gene063364.t1 | MTR_1g040735 | 53.333 | 75 | 26 | 3 | 6 | 72 | 63 | 136 | 3.06e-13 | 60.1 |
| MS.gene063364.t1 | MTR_5g042360 | 51.351 | 74 | 29 | 2 | 6 | 72 | 6 | 79 | 1.71e-12 | 57.0 |
| MS.gene063364.t1 | MTR_1g040775 | 51.351 | 74 | 29 | 2 | 6 | 72 | 6 | 79 | 1.71e-12 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063364.t1 | AT2G32190 | 45.205 | 73 | 37 | 2 | 1 | 72 | 1 | 71 | 1.37e-14 | 62.0 |
| MS.gene063364.t1 | AT2G32210 | 48.333 | 60 | 30 | 1 | 14 | 72 | 12 | 71 | 2.39e-14 | 61.6 |
| MS.gene063364.t1 | AT2G32200 | 44.444 | 63 | 31 | 2 | 14 | 72 | 46 | 108 | 1.24e-11 | 55.5 |
| MS.gene063364.t1 | AT2G32200 | 44.444 | 63 | 31 | 2 | 14 | 72 | 12 | 74 | 2.23e-11 | 53.9 |
Find 26 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGCAGGGGTGATGGTTTC+TGG | 0.154253 | 2.4:-26154095 | MS.gene063364:CDS |
| TTTAATTGGAACACTTTGTT+GGG | 0.371717 | 2.4:+26154122 | None:intergenic |
| AGCTGTTCCACCACCACCTA+TGG | 0.399384 | 2.4:-26154166 | MS.gene063364:CDS |
| ATTATGTTGTTGCTGTGCCC+TGG | 0.400888 | 2.4:-26153634 | MS.gene063364:CDS |
| ACTTTGTTGGGGATCATCCT+TGG | 0.445500 | 2.4:+26154134 | None:intergenic |
| TCGATCAGAGGCAACAATCC+AGG | 0.459282 | 2.4:+26153616 | None:intergenic |
| TTGTTGGGGATCATCCTTGG+AGG | 0.481114 | 2.4:+26154137 | None:intergenic |
| GGGTTGTTCTGACTCACTGC+TGG | 0.483166 | 2.4:+26154192 | None:intergenic |
| TTCCAATTAAAACAACTAGC+AGG | 0.486226 | 2.4:-26154110 | MS.gene063364:CDS |
| GGATAACCCATAGGTGGTGG+TGG | 0.506331 | 2.4:+26154159 | None:intergenic |
| AAAACAACTAGCAGGGGTGA+TGG | 0.528737 | 2.4:-26154102 | MS.gene063364:CDS |
| ATCAATCAACAACAATCTCC+TGG | 0.529469 | 2.4:-26154427 | MS.gene063364:intron |
| GGTGGTGGTGGAACAGCTTG+AGG | 0.543912 | 2.4:+26154171 | None:intergenic |
| CGATCAGAGGCAACAATCCA+GGG | 0.546345 | 2.4:+26153617 | None:intergenic |
| GTGGTGGTGGAACAGCTTGA+GGG | 0.555087 | 2.4:+26154172 | None:intergenic |
| GAGGGATAACCCATAGGTGG+TGG | 0.556470 | 2.4:+26154156 | None:intergenic |
| TCCAATTAAAACAACTAGCA+GGG | 0.562056 | 2.4:-26154109 | MS.gene063364:CDS |
| AGGGGTGATGGTTTCTGGAA+AGG | 0.573043 | 2.4:-26154090 | MS.gene063364:CDS |
| GCTGTTCCACCACCACCTAT+GGG | 0.592890 | 2.4:-26154165 | MS.gene063364:CDS |
| ACCTATGGGTTATCCCTCCA+AGG | 0.593969 | 2.4:-26154151 | MS.gene063364:CDS |
| GTGATGGTTTCTGGAAAGGA+TGG | 0.594474 | 2.4:-26154086 | MS.gene063364:intron |
| TGTTGGGGATCATCCTTGGA+GGG | 0.597882 | 2.4:+26154138 | None:intergenic |
| TTAATTGGAACACTTTGTTG+GGG | 0.602125 | 2.4:+26154123 | None:intergenic |
| CCAATTAAAACAACTAGCAG+GGG | 0.643519 | 2.4:-26154108 | MS.gene063364:CDS |
| TCCTTGGAGGGATAACCCAT+AGG | 0.667495 | 2.4:+26154150 | None:intergenic |
| TTGGAGGGATAACCCATAGG+TGG | 0.675232 | 2.4:+26154153 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAGTTATAGTTATTAATT+AGG | + | chr2.4:26154017-26154036 | None:intergenic | 10.0% |
| !! | ATAATTAAAAAAAACTTACC+AGG | + | chr2.4:26153653-26153672 | None:intergenic | 15.0% |
| !! | CATTTCTATAAGAAATAATT+TGG | - | chr2.4:26153790-26153809 | MS.gene063364:intron | 15.0% |
| !! | CAAAAAAATTAAACGATTCT+AGG | + | chr2.4:26153756-26153775 | None:intergenic | 20.0% |
| !! | CAACTGCAAAAAAAAAAAAA+AGG | + | chr2.4:26154399-26154418 | None:intergenic | 20.0% |
| !! | TATTTATGCTAAAAACAGTT+TGG | + | chr2.4:26154356-26154375 | None:intergenic | 20.0% |
| !!! | GCGATAACTTTTTTTTTTAA+TGG | + | chr2.4:26154219-26154238 | None:intergenic | 20.0% |
| ! | TTTAATTGGAACACTTTGTT+GGG | + | chr2.4:26153940-26153959 | None:intergenic | 25.0% |
| !! | TAACTTCTTTTCCTTTAACT+CGG | + | chr2.4:26154129-26154148 | None:intergenic | 25.0% |
| !!! | AGTTTTTTTCTCCTTTGTTA+GGG | + | chr2.4:26154169-26154188 | None:intergenic | 25.0% |
| !!! | TAGTTTTTTTCTCCTTTGTT+AGG | + | chr2.4:26154170-26154189 | None:intergenic | 25.0% |
| !!! | TTTTAATTGGAACACTTTGT+TGG | + | chr2.4:26153941-26153960 | None:intergenic | 25.0% |
| CTAGGCTTGAATATAAAACA+TGG | + | chr2.4:26153738-26153757 | None:intergenic | 30.0% | |
| GAAATTGAAAACCGAGTTAA+AGG | - | chr2.4:26154115-26154134 | MS.gene063364:CDS | 30.0% | |
| GTTAAAGGAAAAGAAGTTAG+AGG | - | chr2.4:26154130-26154149 | MS.gene063364:CDS | 30.0% | |
| TCCAATTAAAACAACTAGCA+GGG | - | chr2.4:26153950-26153969 | MS.gene063364:intron | 30.0% | |
| TTAATTGGAACACTTTGTTG+GGG | + | chr2.4:26153939-26153958 | None:intergenic | 30.0% | |
| TTCCAATTAAAACAACTAGC+AGG | - | chr2.4:26153949-26153968 | MS.gene063364:intron | 30.0% | |
| !!! | TTTTTTTTTTGCAGTTGTGC+TGG | - | chr2.4:26154402-26154421 | MS.gene063364:intron | 30.0% |
| ATCAATCAACAACAATCTCC+TGG | - | chr2.4:26153632-26153651 | MS.gene063364:CDS | 35.0% | |
| CCAATTAAAACAACTAGCAG+GGG | - | chr2.4:26153951-26153970 | MS.gene063364:intron | 35.0% | |
| CGAAATACACAACACAAGTT+AGG | + | chr2.4:26153828-26153847 | None:intergenic | 35.0% | |
| TTAGGTTCAAACCCTAACAA+AGG | - | chr2.4:26154155-26154174 | MS.gene063364:CDS | 35.0% | |
| !! | GAAAAGAAGTTAGAGGTGTT+AGG | - | chr2.4:26154137-26154156 | MS.gene063364:CDS | 35.0% |
| !!! | CCCCTGCTAGTTGTTTTAAT+TGG | + | chr2.4:26153954-26153973 | None:intergenic | 40.0% |
| AAAACAACTAGCAGGGGTGA+TGG | - | chr2.4:26153957-26153976 | MS.gene063364:intron | 45.0% | |
| ACTTTGTTGGGGATCATCCT+TGG | + | chr2.4:26153928-26153947 | None:intergenic | 45.0% | |
| ATTATGTTGTTGCTGTGCCC+TGG | - | chr2.4:26154425-26154444 | MS.gene063364:intron | 45.0% | |
| ! | GTGATGGTTTCTGGAAAGGA+TGG | - | chr2.4:26153973-26153992 | MS.gene063364:intron | 45.0% |
| TCCTTGGAGGGATAACCCAT+AGG | + | chr2.4:26153912-26153931 | None:intergenic | 50.0% | |
| TGTTGGGGATCATCCTTGGA+GGG | + | chr2.4:26153924-26153943 | None:intergenic | 50.0% | |
| TTGGAGGGATAACCCATAGG+TGG | + | chr2.4:26153909-26153928 | None:intergenic | 50.0% | |
| TTGTTGGGGATCATCCTTGG+AGG | + | chr2.4:26153925-26153944 | None:intergenic | 50.0% | |
| ! | ACCTATGGGTTATCCCTCCA+AGG | - | chr2.4:26153908-26153927 | MS.gene063364:intron | 50.0% |
| ! | AGGGGTGATGGTTTCTGGAA+AGG | - | chr2.4:26153969-26153988 | MS.gene063364:intron | 50.0% |
| AGCTGTTCCACCACCACCTA+TGG | - | chr2.4:26153893-26153912 | MS.gene063364:intron | 55.0% | |
| GAGGGATAACCCATAGGTGG+TGG | + | chr2.4:26153906-26153925 | None:intergenic | 55.0% | |
| GCTGTTCCACCACCACCTAT+GGG | - | chr2.4:26153894-26153913 | MS.gene063364:intron | 55.0% | |
| GGGTTGTTCTGACTCACTGC+TGG | + | chr2.4:26153870-26153889 | None:intergenic | 55.0% | |
| ! | CTAGCAGGGGTGATGGTTTC+TGG | - | chr2.4:26153964-26153983 | MS.gene063364:intron | 55.0% |
| ! | GGATAACCCATAGGTGGTGG+TGG | + | chr2.4:26153903-26153922 | None:intergenic | 55.0% |
| !! | GTGGTGGTGGAACAGCTTGA+GGG | + | chr2.4:26153890-26153909 | None:intergenic | 55.0% |
| !! | GGTGGTGGTGGAACAGCTTG+AGG | + | chr2.4:26153891-26153910 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 26153620 | 26154461 | 26153620 | ID=MS.gene063364 |
| chr2.4 | mRNA | 26153620 | 26154461 | 26153620 | ID=MS.gene063364.t1;Parent=MS.gene063364 |
| chr2.4 | exon | 26154428 | 26154461 | 26154428 | ID=MS.gene063364.t1.exon1;Parent=MS.gene063364.t1 |
| chr2.4 | CDS | 26154428 | 26154461 | 26154428 | ID=cds.MS.gene063364.t1;Parent=MS.gene063364.t1 |
| chr2.4 | exon | 26154087 | 26154225 | 26154087 | ID=MS.gene063364.t1.exon2;Parent=MS.gene063364.t1 |
| chr2.4 | CDS | 26154087 | 26154225 | 26154087 | ID=cds.MS.gene063364.t1;Parent=MS.gene063364.t1 |
| chr2.4 | exon | 26153620 | 26153665 | 26153620 | ID=MS.gene063364.t1.exon3;Parent=MS.gene063364.t1 |
| chr2.4 | CDS | 26153620 | 26153665 | 26153620 | ID=cds.MS.gene063364.t1;Parent=MS.gene063364.t1 |
| Gene Sequence |
| Protein sequence |
