AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063557

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTGTGAATAATATTATTT+TGG 0.138672 5.2:+14555239 None:intergenic
CCAAATCTTTGTATTGTTAC+TGG 0.215233 5.2:-14556042 MS.gene063557:intron
TTGGTCAAGGTTGCTGATTT+TGG 0.233442 5.2:-14554755 MS.gene063557:CDS
CACCAACTGCTGCTTGTAAT+GGG 0.280382 5.2:+14553725 None:intergenic
GAAAAGGCTGGTCTCTCCTT+TGG 0.340978 5.2:+14553526 None:intergenic
AACTGAAAACATCTGCCTTC+TGG 0.342226 5.2:+14553926 None:intergenic
CCAGTAACAATACAAAGATT+TGG 0.347163 5.2:+14556042 None:intergenic
AAGAATGTTGTTCAGTTCAT+TGG 0.353356 5.2:-14556081 MS.gene063557:CDS
TGTAGTAGTTCAGAAATTCT+TGG 0.367558 5.2:+14553568 None:intergenic
ACATCTGCCTTCTGGTCATA+TGG 0.369157 5.2:+14553934 None:intergenic
GGAGTGATGACAGCTGAAAC+TGG 0.383152 5.2:-14554707 MS.gene063557:CDS
ACACCAACTGCTGCTTGTAA+TGG 0.418160 5.2:+14553724 None:intergenic
GTGCAAATAGTTCATTCCCT+TGG 0.427844 5.2:+14555261 None:intergenic
GCCAGAGTGCAAACTCAGTC+TGG 0.441380 5.2:-14554728 MS.gene063557:CDS
AGTTCATTGGTGCTTGTACT+CGG 0.441416 5.2:-14556068 MS.gene063557:CDS
AATTTCAATTATTTCAGAAA+AGG 0.452807 5.2:+14553510 None:intergenic
ACCAAAATAATATTATTCAC+AGG 0.461164 5.2:-14555240 MS.gene063557:CDS
CTGAACTACTACAGCGGTGC+TGG 0.464205 5.2:-14553558 MS.gene063557:CDS
CATTCCCTTGGAAACATCAA+TGG 0.470441 5.2:+14555273 None:intergenic
TCAATTATTTCAGAAAAGGC+TGG 0.482656 5.2:+14553514 None:intergenic
TCTCCAACACATAGCAAAAG+AGG 0.496421 5.2:-14553488 MS.gene063557:intron
TAAATTGCTATTAGGGTCTA+CGG 0.513896 5.2:-14553615 MS.gene063557:intron
AATGGGGTCAAGTAAGAGTA+AGG 0.518427 5.2:+14553742 None:intergenic
AGTAGCCATTGATGTTTCCA+AGG 0.531408 5.2:-14555278 MS.gene063557:CDS
CAAGACTGCCAATCTTCTGA+TGG 0.535133 5.2:-14555212 MS.gene063557:CDS
AGCAGTTGGTGTGGTGCAGA+AGG 0.540517 5.2:-14553713 MS.gene063557:intron
CTGAAACTGGAACATACCGC+TGG 0.555978 5.2:-14554694 MS.gene063557:CDS
ATGGGTATCAATATCAGTGA+TGG 0.582525 5.2:+14553297 None:intergenic
TTCCTCTCATCACTTAGGCG+TGG 0.584086 5.2:-14553320 MS.gene063557:CDS
TCCAGACTGAGTTTGCACTC+TGG 0.592327 5.2:+14554727 None:intergenic
GTTTCAGAGTTTATGTCGAG+AGG 0.597271 5.2:-14555361 MS.gene063557:intron
GTAACAATACAAAGATTTGG+AGG 0.600790 5.2:+14556045 None:intergenic
TCAGAAGATTGGCAGTCTTG+AGG 0.601551 5.2:+14555215 None:intergenic
ATACCGCTGGATGGCTCCTG+AGG 0.602142 5.2:-14554681 MS.gene063557:intron
AACTGGAACATACCGCTGGA+TGG 0.602404 5.2:-14554690 MS.gene063557:CDS
ACCCCATTACAAGCAGCAGT+TGG 0.604156 5.2:-14553727 MS.gene063557:CDS
TATCCTTCAACAGTTGGTCA+AGG 0.605155 5.2:-14554768 MS.gene063557:intron
CCAAAATAATATTATTCACA+GGG 0.607549 5.2:-14555239 MS.gene063557:CDS
GTAGCCATTGATGTTTCCAA+GGG 0.609070 5.2:-14555277 MS.gene063557:CDS
ACCAACTGCTGCTTGTAATG+GGG 0.611610 5.2:+14553726 None:intergenic
ATTACAAGCAGCAGTTGGTG+TGG 0.628172 5.2:-14553722 MS.gene063557:CDS
GTGCTGGCAACAAGATCCAA+AGG 0.636417 5.2:-14553542 MS.gene063557:CDS
ACGGTTTCCTCTCATCACTT+AGG 0.644133 5.2:-14553325 MS.gene063557:CDS
ACACAAACCATATGACCAGA+AGG 0.644562 5.2:-14553941 MS.gene063557:CDS
CATAAAGATAAGTCATCACA+CGG 0.667424 5.2:-14553344 MS.gene063557:CDS
GAATTTCTGAACTACTACAG+CGG 0.681521 5.2:-14553564 MS.gene063557:CDS
GGCCACGCCTAAGTGATGAG+AGG 0.683467 5.2:+14553318 None:intergenic
ATACCTCAGGAGCCATCCAG+CGG 0.707173 5.2:+14554678 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAAATAGAATATATAA+TGG + chr5.2:14555633-14555652 None:intergenic 10.0%
!!! ATGTTAATGTTTTTTTTATA+TGG - chr5.2:14555080-14555099 MS.gene063557:intron 10.0%
!!! ATTTTATTTTAAATGTATCA+GGG - chr5.2:14554865-14554884 MS.gene063557:intron 10.0%
!!! TATTTTATTTTAAATGTATC+AGG - chr5.2:14554864-14554883 MS.gene063557:intron 10.0%
!! AAAAAAGATTTATTCTTTCT+GGG - chr5.2:14555195-14555214 MS.gene063557:intron 15.0%
!! AATTTCAATTATTTCAGAAA+AGG + chr5.2:14555897-14555916 None:intergenic 15.0%
!! TAAAAAAGATTTATTCTTTC+TGG - chr5.2:14555194-14555213 MS.gene063557:intron 15.0%
!! TAACATCATAAAAAAAAAAG+AGG - chr5.2:14555947-14555966 MS.gene063557:intron 15.0%
!!! TTTTATTTTAAATGTATCAG+GGG - chr5.2:14554866-14554885 MS.gene063557:intron 15.0%
!!! TTTTTTTCTTTTCATAATTG+AGG - chr5.2:14555170-14555189 MS.gene063557:intron 15.0%
!! AACAAATTCTATAATCAACT+AGG + chr5.2:14554431-14554450 None:intergenic 20.0%
!! ACCAAAATAATATTATTCAC+AGG - chr5.2:14554164-14554183 MS.gene063557:intron 20.0%
!! AGAAAGAAAAAAAACAGAAA+AGG + chr5.2:14555377-14555396 None:intergenic 20.0%
!! ATATTTGATTCACATTGTTT+GGG - chr5.2:14555103-14555122 MS.gene063557:intron 20.0%
!! ATGTTAAGATTTGTGTAATT+AGG - chr5.2:14553756-14553775 MS.gene063557:intron 20.0%
!! CAAATATCTATAAACGATAT+TGG + chr5.2:14553636-14553655 None:intergenic 20.0%
!! CAATATCGTTTATAGATATT+TGG - chr5.2:14553634-14553653 MS.gene063557:intron 20.0%
!! CCAAAATAATATTATTCACA+GGG - chr5.2:14554165-14554184 MS.gene063557:intron 20.0%
!! GAAACAGAAAATTGTTAAAT+AGG + chr5.2:14556011-14556030 None:intergenic 20.0%
!! TATTAATTGCTAAGATGATT+TGG - chr5.2:14553971-14553990 MS.gene063557:intron 20.0%
!! TTATCCTAATCTCTTAAATT+AGG - chr5.2:14554974-14554993 MS.gene063557:intron 20.0%
!!! AATAAAATGTTGAGATGTTT+TGG + chr5.2:14554596-14554615 None:intergenic 20.0%
!!! ATAAAATGTTGAGATGTTTT+GGG + chr5.2:14554595-14554614 None:intergenic 20.0%
!!! ATATCAAACAAATTCTAGAA+AGG + chr5.2:14553601-14553620 None:intergenic 20.0%
!!! GCTTATATGATAATGATATT+TGG - chr5.2:14555348-14555367 MS.gene063557:CDS 20.0%
!!! TATCAAACAAATTCTAGAAA+GGG + chr5.2:14553600-14553619 None:intergenic 20.0%
!!! TTTATTTTAAATGTATCAGG+GGG - chr5.2:14554867-14554886 MS.gene063557:intron 20.0%
! AAGTAAAATGCAAGTAAAAC+TGG - chr5.2:14554915-14554934 MS.gene063557:intron 25.0%
! AATAACAATGAAAATGCAAC+AGG + chr5.2:14554264-14554283 None:intergenic 25.0%
! AATCTCTTAAATTAGGTTCT+TGG - chr5.2:14554981-14555000 MS.gene063557:intron 25.0%
! AGAACCTAATTTAAGAGATT+AGG + chr5.2:14554981-14555000 None:intergenic 25.0%
! ATAATTTACTACTAGAACTG+TGG - chr5.2:14554464-14554483 MS.gene063557:intron 25.0%
! GATATTTGATTCACATTGTT+TGG - chr5.2:14555102-14555121 MS.gene063557:intron 25.0%
!! AATTCTTGGATGTGTATTTT+TGG + chr5.2:14555825-14555844 None:intergenic 25.0%
!! ATTCTTCTGCTTGATATTTT+GGG - chr5.2:14554771-14554790 MS.gene063557:intron 25.0%
!! ATTCTTGGATGTGTATTTTT+GGG + chr5.2:14555824-14555843 None:intergenic 25.0%
!! CCCTGTGAATAATATTATTT+TGG + chr5.2:14554168-14554187 None:intergenic 25.0%
!! GTTTCATACTTTAGTTCAAA+GGG - chr5.2:14553426-14553445 MS.gene063557:intron 25.0%
!! TATTCTTCTGCTTGATATTT+TGG - chr5.2:14554770-14554789 MS.gene063557:intron 25.0%
!! TGTTGCATTTTCATTGTTAT+TGG - chr5.2:14554263-14554282 MS.gene063557:intron 25.0%
!! TTTCAATATTTTGTGAACTC+CGG + chr5.2:14554949-14554968 None:intergenic 25.0%
!!! ACCATGGACAATATTTTTAA+GGG + chr5.2:14555011-14555030 None:intergenic 25.0%
!!! AGAATATATAATGGATTAGC+AGG + chr5.2:14555624-14555643 None:intergenic 25.0%
!!! TTACTTCTGTTGGTAATATT+TGG - chr5.2:14554295-14554314 MS.gene063557:intron 25.0%
!!! TTATTTTAAATGTATCAGGG+GGG - chr5.2:14554868-14554887 MS.gene063557:intron 25.0%
AAGAATGTTGTTCAGTTCAT+TGG - chr5.2:14553323-14553342 MS.gene063557:CDS 30.0%
AATCAACTAGGAATTGAACA+TGG + chr5.2:14554419-14554438 None:intergenic 30.0%
ACCCTTAAAAATATTGTCCA+TGG - chr5.2:14555007-14555026 MS.gene063557:intron 30.0%
ACTCATATCAGTTAGAGATA+CGG - chr5.2:14553845-14553864 MS.gene063557:intron 30.0%
AGCATCACAGTTGAATTATT+AGG + chr5.2:14554012-14554031 None:intergenic 30.0%
AGGCAATACATTAGTGATAT+AGG + chr5.2:14554361-14554380 None:intergenic 30.0%
AGTAAAACTGGTATTCAAAC+CGG - chr5.2:14554927-14554946 MS.gene063557:intron 30.0%
ATACTCGATGCAATACAAAT+AGG + chr5.2:14553952-14553971 None:intergenic 30.0%
ATCAACTAGGAATTGAACAT+GGG + chr5.2:14554418-14554437 None:intergenic 30.0%
ATGCAATACAAATAGGAACT+TGG + chr5.2:14553945-14553964 None:intergenic 30.0%
ATTGTTAAATAGGCACAACT+TGG + chr5.2:14556001-14556020 None:intergenic 30.0%
CAATAAAATTATCCGCAAAG+AGG + chr5.2:14555729-14555748 None:intergenic 30.0%
CAATGTAGTCTTTAATGCTT+GGG - chr5.2:14553869-14553888 MS.gene063557:intron 30.0%
CATAAAGATAAGTCATCACA+CGG - chr5.2:14556060-14556079 MS.gene063557:CDS 30.0%
CCAAATCTTTGTATTGTTAC+TGG - chr5.2:14553362-14553381 MS.gene063557:CDS 30.0%
CCAGTAACAATACAAAGATT+TGG + chr5.2:14553365-14553384 None:intergenic 30.0%
GTAACAATACAAAGATTTGG+AGG + chr5.2:14553362-14553381 None:intergenic 30.0%
TAACCTTCAAGTCAAATCAA+CGG + chr5.2:14555439-14555458 None:intergenic 30.0%
TCAATTATTTCAGAAAAGGC+TGG + chr5.2:14555893-14555912 None:intergenic 30.0%
TGGGAAAGTAAAAAATGCAA+TGG + chr5.2:14554399-14554418 None:intergenic 30.0%
TGTAGTAGTTCAGAAATTCT+TGG + chr5.2:14555839-14555858 None:intergenic 30.0%
TTACACACAATAACTCTATG+TGG - chr5.2:14555764-14555783 MS.gene063557:intron 30.0%
TTCAAGAAAAATCATACCTC+AGG + chr5.2:14554742-14554761 None:intergenic 30.0%
TTGTATTGTTACTGGTAAGT+AGG - chr5.2:14553370-14553389 MS.gene063557:intron 30.0%
TTTGTATTCTTTCCTCTTTG+CGG - chr5.2:14555714-14555733 MS.gene063557:intron 30.0%
! ATGTGGCTTAAATTGCTATT+AGG - chr5.2:14555781-14555800 MS.gene063557:intron 30.0%
! CGTTTCATACTTTAGTTCAA+AGG - chr5.2:14553425-14553444 MS.gene063557:intron 30.0%
! TAAATTGCTATTAGGGTCTA+CGG - chr5.2:14555789-14555808 MS.gene063557:intron 30.0%
! TGTGGCTTAAATTGCTATTA+GGG - chr5.2:14555782-14555801 MS.gene063557:intron 30.0%
! TTTTAAATTCTGCTTCACCA+TGG + chr5.2:14555027-14555046 None:intergenic 30.0%
! TTTTCTGTTTCATGTTTGTC+AGG - chr5.2:14556020-14556039 MS.gene063557:intron 30.0%
!! ATCCAAACTCTAAAACTTCA+AGG + chr5.2:14555320-14555339 None:intergenic 30.0%
!! CACCATGGACAATATTTTTA+AGG + chr5.2:14555012-14555031 None:intergenic 30.0%
!! GTCTATGTTTTTACTTCTGT+TGG - chr5.2:14554285-14554304 MS.gene063557:intron 30.0%
!!! ATGGCTACTTTTAGTAAAGA+TGG + chr5.2:14554115-14554134 None:intergenic 30.0%
!!! TGTGTATTTTTGGGAATTGT+AGG + chr5.2:14555815-14555834 None:intergenic 30.0%
AAAAACATACACTCATGCTC+AGG + chr5.2:14553722-14553741 None:intergenic 35.0%
AGTATCAATGTGTGCATTCA+TGG + chr5.2:14554240-14554259 None:intergenic 35.0%
ATATCATTGTGCAAAGCGAT+TGG - chr5.2:14554796-14554815 MS.gene063557:intron 35.0%
CCAAGCATTAAAGACTACAT+TGG + chr5.2:14553871-14553890 None:intergenic 35.0%
CCAATGTAGTCTTTAATGCT+TGG - chr5.2:14553868-14553887 MS.gene063557:intron 35.0%
GAATTTCTGAACTACTACAG+CGG - chr5.2:14555840-14555859 MS.gene063557:intron 35.0%
TATCATTGTGCAAAGCGATT+GGG - chr5.2:14554797-14554816 MS.gene063557:intron 35.0%
TATTGCCTATCTCGATTGTT+GGG - chr5.2:14554373-14554392 MS.gene063557:intron 35.0%
! AAGGCAGATGTTTTCAGTTT+TGG - chr5.2:14555482-14555501 MS.gene063557:intron 35.0%
! ATTTTCGTCCATCAGAAGAT+TGG + chr5.2:14554203-14554222 None:intergenic 35.0%
! CAAACAAATTCTAGAAAGGG+AGG + chr5.2:14553597-14553616 None:intergenic 35.0%
! GATTTTCTGCACAGACAAAA+GGG - chr5.2:14554076-14554095 MS.gene063557:intron 35.0%
! GGCAAAGTATTTACATTGCT+TGG - chr5.2:14553655-14553674 MS.gene063557:intron 35.0%
! GGTGAAGCAGAATTTAAAAC+TGG - chr5.2:14555028-14555047 MS.gene063557:intron 35.0%
! TGATTTTCTGCACAGACAAA+AGG - chr5.2:14554075-14554094 MS.gene063557:intron 35.0%
!! GTTTGGATATTTTTCAGCAG+TGG - chr5.2:14554825-14554844 MS.gene063557:intron 35.0%
!! TTACCTCTTTTGCTATGTGT+TGG + chr5.2:14555922-14555941 None:intergenic 35.0%
!! TTTCCGTTGATTTGACTTGA+AGG - chr5.2:14555433-14555452 MS.gene063557:intron 35.0%
!!! TGCCTTGAAGTTTTAGAGTT+TGG - chr5.2:14555315-14555334 MS.gene063557:CDS 35.0%
AAAGCGATTGGGTGATAGTT+TGG - chr5.2:14554808-14554827 MS.gene063557:intron 40.0%
AACTGAAAACATCTGCCTTC+TGG + chr5.2:14555481-14555500 None:intergenic 40.0%
AATGCATTACATGCTTGGCA+AGG + chr5.2:14555227-14555246 None:intergenic 40.0%
AATGGGGTCAAGTAAGAGTA+AGG + chr5.2:14555665-14555684 None:intergenic 40.0%
ACAAGGCTTCCAACATTGTA+GGG + chr5.2:14553568-14553587 None:intergenic 40.0%
ACACAAACCATATGACCAGA+AGG - chr5.2:14555463-14555482 MS.gene063557:intron 40.0%
AGAGCATATCCTTCAACAGT+TGG - chr5.2:14554630-14554649 MS.gene063557:intron 40.0%
AGTAGCCATTGATGTTTCCA+AGG - chr5.2:14554126-14554145 MS.gene063557:intron 40.0%
ATGTATGCAGTATCCACCAT+CGG + chr5.2:14553531-14553550 None:intergenic 40.0%
CATTCCCTTGGAAACATCAA+TGG + chr5.2:14554134-14554153 None:intergenic 40.0%
GTAGCCATTGATGTTTCCAA+GGG - chr5.2:14554127-14554146 MS.gene063557:intron 40.0%
GTATTGCCTATCTCGATTGT+TGG - chr5.2:14554372-14554391 MS.gene063557:intron 40.0%
GTGATATAGGATTGCAACTG+AGG + chr5.2:14554348-14554367 None:intergenic 40.0%
GTGCAAATAGTTCATTCCCT+TGG + chr5.2:14554146-14554165 None:intergenic 40.0%
GTTTCAGAGTTTATGTCGAG+AGG - chr5.2:14554043-14554062 MS.gene063557:intron 40.0%
TATCCTTCAACAGTTGGTCA+AGG - chr5.2:14554636-14554655 MS.gene063557:intron 40.0%
TCTCCAACACATAGCAAAAG+AGG - chr5.2:14555916-14555935 MS.gene063557:intron 40.0%
TTACTACACTGTTACTGCGT+CGG - chr5.2:14553479-14553498 MS.gene063557:intron 40.0%
TTGTTACTGGTAAGTAGGAG+AGG - chr5.2:14553375-14553394 MS.gene063557:intron 40.0%
! AGTTCATTGGTGCTTGTACT+CGG - chr5.2:14553336-14553355 MS.gene063557:CDS 40.0%
! ATCTGAAGCATGGACAAATG+TGG + chr5.2:14553786-14553805 None:intergenic 40.0%
! TCTGAAGCATGGACAAATGT+GGG + chr5.2:14553785-14553804 None:intergenic 40.0%
! TTCGAGATGAGTTGCAGTTT+TGG + chr5.2:14554508-14554527 None:intergenic 40.0%
!!! CAGTTTTGGAATAGCACTCT+GGG - chr5.2:14555496-14555515 MS.gene063557:intron 40.0%
!!! TCAGTTTTGGAATAGCACTC+TGG - chr5.2:14555495-14555514 MS.gene063557:intron 40.0%
!!! TGGTCAAGGTTGCTGATTTT+GGG - chr5.2:14554650-14554669 MS.gene063557:intron 40.0%
!!! TTGGTCAAGGTTGCTGATTT+TGG - chr5.2:14554649-14554668 MS.gene063557:intron 40.0%
AACCCCAAAAGCTAGCTCAA+TGG + chr5.2:14553903-14553922 None:intergenic 45.0%
AAGCCCAAAAGCTAGCTCAA+GGG + chr5.2:14553692-14553711 None:intergenic 45.0%
AATGGCCCAACAATCGAGAT+AGG + chr5.2:14554381-14554400 None:intergenic 45.0%
ACATCTGCCTTCTGGTCATA+TGG + chr5.2:14555473-14555492 None:intergenic 45.0%
ACCCCAAAAGCTAGCTCAAT+GGG + chr5.2:14553902-14553921 None:intergenic 45.0%
ACGGTTTCCTCTCATCACTT+AGG - chr5.2:14556079-14556098 MS.gene063557:CDS 45.0%
CAACCTTGACCAACTGTTGA+AGG + chr5.2:14554642-14554661 None:intergenic 45.0%
CAAGACTGCCAATCTTCTGA+TGG - chr5.2:14554192-14554211 MS.gene063557:intron 45.0%
CACAAGGCTTCCAACATTGT+AGG + chr5.2:14553569-14553588 None:intergenic 45.0%
CTTCCAACATTGTAGGGTGT+GGG + chr5.2:14553562-14553581 None:intergenic 45.0%
GCGACAATGCATTACATGCT+TGG + chr5.2:14555232-14555251 None:intergenic 45.0%
GTAACCTTGTCCTTGCATCA+AGG + chr5.2:14553463-14553482 None:intergenic 45.0%
TCAAGTAAGAGTAAGGCAGC+TGG + chr5.2:14555658-14555677 None:intergenic 45.0%
TCAGAAGATTGGCAGTCTTG+AGG + chr5.2:14554192-14554211 None:intergenic 45.0%
TGTCCATGCTTCAGATGTCT+AGG - chr5.2:14553790-14553809 MS.gene063557:intron 45.0%
! ACACCAACTGCTGCTTGTAA+TGG + chr5.2:14555683-14555702 None:intergenic 45.0%
! ACCAACTGCTGCTTGTAATG+GGG + chr5.2:14555681-14555700 None:intergenic 45.0%
! ATTACAAGCAGCAGTTGGTG+TGG - chr5.2:14555682-14555701 MS.gene063557:intron 45.0%
! CACCAACTGCTGCTTGTAAT+GGG + chr5.2:14555682-14555701 None:intergenic 45.0%
!!! ACCCATTGAGCTAGCTTTTG+GGG - chr5.2:14553898-14553917 MS.gene063557:CDS 45.0%
!!! CACCCATTGAGCTAGCTTTT+GGG - chr5.2:14553897-14553916 MS.gene063557:CDS 45.0%
!!! CTAGCTTTTGGGGTTGAGTT+GGG - chr5.2:14553908-14553927 MS.gene063557:CDS 45.0%
!!! GGTCAAGGTTGCTGATTTTG+GGG - chr5.2:14554651-14554670 MS.gene063557:intron 45.0%
!! AAGAAAAAAAAAAAAAAAAA+AGG + chr5.2:14555160-14555179 None:intergenic 5.0%
AAAAGCTAGCTCAAGGGGTG+AGG + chr5.2:14553686-14553705 None:intergenic 50.0%
AACTGGAACATACCGCTGGA+TGG - chr5.2:14554714-14554733 MS.gene063557:CDS 50.0%
ACACTGTTACTGCGTCGGAA+TGG - chr5.2:14553484-14553503 MS.gene063557:intron 50.0%
ACCCCATTACAAGCAGCAGT+TGG - chr5.2:14555677-14555696 MS.gene063557:intron 50.0%
AGAAAGGGAGGTTGTGCACA+AGG + chr5.2:14553585-14553604 None:intergenic 50.0%
AGCCCAAAAGCTAGCTCAAG+GGG + chr5.2:14553691-14553710 None:intergenic 50.0%
AGGCCATACACCTTAGTGTG+AGG - chr5.2:14553810-14553829 MS.gene063557:intron 50.0%
CAACATTGTAGGGTGTGGGA+GGG + chr5.2:14553558-14553577 None:intergenic 50.0%
CAAGCCCAAAAGCTAGCTCA+AGG + chr5.2:14553693-14553712 None:intergenic 50.0%
CATCAAACCCCCTCACACTA+AGG + chr5.2:14553823-14553842 None:intergenic 50.0%
CCAAAAGCTAGCTCAATGGG+TGG + chr5.2:14553899-14553918 None:intergenic 50.0%
CCATACACCTTAGTGTGAGG+GGG - chr5.2:14553813-14553832 MS.gene063557:intron 50.0%
CTGAAACTGGAACATACCGC+TGG - chr5.2:14554710-14554729 MS.gene063557:CDS 50.0%
GAAGCATGGACAAATGTGGG+TGG + chr5.2:14553782-14553801 None:intergenic 50.0%
GCCATACACCTTAGTGTGAG+GGG - chr5.2:14553812-14553831 MS.gene063557:intron 50.0%
GCTTCCAACATTGTAGGGTG+TGG + chr5.2:14553563-14553582 None:intergenic 50.0%
GGAGTGATGACAGCTGAAAC+TGG - chr5.2:14554697-14554716 MS.gene063557:CDS 50.0%
GGCCATACACCTTAGTGTGA+GGG - chr5.2:14553811-14553830 MS.gene063557:intron 50.0%
GGTTTAGCAACCTCTTCCGA+TGG - chr5.2:14553512-14553531 MS.gene063557:CDS 50.0%
TCCAGACTGAGTTTGCACTC+TGG + chr5.2:14554680-14554699 None:intergenic 50.0%
TTAGCAACCTCTTCCGATGG+TGG - chr5.2:14553515-14553534 MS.gene063557:CDS 50.0%
TTCCTCTCATCACTTAGGCG+TGG - chr5.2:14556084-14556103 MS.gene063557:CDS 50.0%
! GTGCTGGCAACAAGATCCAA+AGG - chr5.2:14555862-14555881 MS.gene063557:intron 50.0%
! TGGCCTAGACATCTGAAGCA+TGG + chr5.2:14553796-14553815 None:intergenic 50.0%
!! CTGGGAGCTTTTAACTGGAG+AGG - chr5.2:14555514-14555533 MS.gene063557:intron 50.0%
!! GAAAAGGCTGGTCTCTCCTT+TGG + chr5.2:14555881-14555900 None:intergenic 50.0%
!! GCACTCTGGGAGCTTTTAAC+TGG - chr5.2:14555509-14555528 MS.gene063557:intron 50.0%
!!! CACCCCTTGAGCTAGCTTTT+GGG - chr5.2:14553686-14553705 MS.gene063557:intron 50.0%
!!! CCACCCATTGAGCTAGCTTT+TGG - chr5.2:14553896-14553915 MS.gene063557:CDS 50.0%
!!! CTTGAGCTAGCTTTTGGGCT+TGG - chr5.2:14553691-14553710 MS.gene063557:intron 50.0%
!!! GCTAGCTTTTGGGGTTGAGT+TGG - chr5.2:14553907-14553926 MS.gene063557:CDS 50.0%
!!! TCACCCCTTGAGCTAGCTTT+TGG - chr5.2:14553685-14553704 MS.gene063557:intron 50.0%
AGGGGGGAAGACTTTGTGTG+GGG - chr5.2:14554884-14554903 MS.gene063557:intron 55.0%
ATACCTCAGGAGCCATCCAG+CGG + chr5.2:14554729-14554748 None:intergenic 55.0%
CAGGGGGGAAGACTTTGTGT+GGG - chr5.2:14554883-14554902 MS.gene063557:intron 55.0%
CCAACATTGTAGGGTGTGGG+AGG + chr5.2:14553559-14553578 None:intergenic 55.0%
CCCCCTCACACTAAGGTGTA+TGG + chr5.2:14553816-14553835 None:intergenic 55.0%
CCTCCCACACCCTACAATGT+TGG - chr5.2:14553556-14553575 MS.gene063557:CDS 55.0%
CGAGCCTTGATGCAAGGACA+AGG - chr5.2:14553456-14553475 MS.gene063557:intron 55.0%
CTGAACTACTACAGCGGTGC+TGG - chr5.2:14555846-14555865 MS.gene063557:intron 55.0%
GCAGTATCCACCATCGGAAG+AGG + chr5.2:14553525-14553544 None:intergenic 55.0%
GCCAGAGTGCAAACTCAGTC+TGG - chr5.2:14554676-14554695 MS.gene063557:intron 55.0%
TCAGGGGGGAAGACTTTGTG+TGG - chr5.2:14554882-14554901 MS.gene063557:intron 55.0%
! AGCAGTTGGTGTGGTGCAGA+AGG - chr5.2:14555691-14555710 MS.gene063557:intron 55.0%
! TACTGCGTCGGAATGGACTC+TGG - chr5.2:14553491-14553510 MS.gene063557:CDS 55.0%
ATACCGCTGGATGGCTCCTG+AGG - chr5.2:14554723-14554742 MS.gene063557:CDS 60.0%
GAGCTGCGAGCCTTGATGCA+AGG - chr5.2:14553450-14553469 MS.gene063557:intron 60.0%
GGCCACGCCTAAGTGATGAG+AGG + chr5.2:14556089-14556108 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 14553310 14556116 14553310 ID=MS.gene063557
chr5.2 mRNA 14553310 14556116 14553310 ID=MS.gene063557.t1;Parent=MS.gene063557
chr5.2 exon 14556043 14556116 14556043 ID=MS.gene063557.t1.exon1;Parent=MS.gene063557.t1
chr5.2 CDS 14556043 14556116 14556043 ID=cds.MS.gene063557.t1;Parent=MS.gene063557.t1
chr5.2 exon 14555201 14555376 14555201 ID=MS.gene063557.t1.exon2;Parent=MS.gene063557.t1
chr5.2 CDS 14555201 14555376 14555201 ID=cds.MS.gene063557.t1;Parent=MS.gene063557.t1
chr5.2 exon 14554682 14554777 14554682 ID=MS.gene063557.t1.exon3;Parent=MS.gene063557.t1
chr5.2 CDS 14554682 14554777 14554682 ID=cds.MS.gene063557.t1;Parent=MS.gene063557.t1
chr5.2 exon 14553891 14553971 14553891 ID=MS.gene063557.t1.exon4;Parent=MS.gene063557.t1
chr5.2 CDS 14553891 14553971 14553891 ID=cds.MS.gene063557.t1;Parent=MS.gene063557.t1
chr5.2 exon 14553714 14553767 14553714 ID=MS.gene063557.t1.exon5;Parent=MS.gene063557.t1
chr5.2 CDS 14553714 14553767 14553714 ID=cds.MS.gene063557.t1;Parent=MS.gene063557.t1
chr5.2 exon 14553489 14553623 14553489 ID=MS.gene063557.t1.exon6;Parent=MS.gene063557.t1
chr5.2 CDS 14553489 14553623 14553489 ID=cds.MS.gene063557.t1;Parent=MS.gene063557.t1
chr5.2 exon 14553310 14553384 14553310 ID=MS.gene063557.t1.exon7;Parent=MS.gene063557.t1
chr5.2 CDS 14553310 14553384 14553310 ID=cds.MS.gene063557.t1;Parent=MS.gene063557.t1
Gene Sequence

>MS.gene063557

GAAGATTCGACACAAGAATGTTGTTCAGTTCATTGGTGCTTGTACTCGGCCTCCAAATCTTTGTATTGTTACTGGTAAGTAGGAGAGGATTTTGAGAACTTAAGATTTATGTTTACGTTTCATACTTTAGTTCAAAGGGTGAGCTGCGAGCCTTGATGCAAGGACAAGGTTACTACACTGTTACTGCGTCGGAATGGACTCTGGTTTAGCAACCTCTTCCGATGGTGGATACTGCATACATTTGACCCTCCCACACCCTACAATGTTGGAAGCCTTGTGCACAACCTCCCTTTCTAGAATTTGTTTGATATCTCTGATGCTATCCAATATCGTTTATAGATATTTGGCAAAGTATTTACATTGCTTGGACAATCCTCACCCCTTGAGCTAGCTTTTGGGCTTGGACAGTCCTGAGCATGAGTGTATGTTTTTTCTATAAGCAATGTATGTTAAGATTTGTGTAATTAGGCCACCCACATTTGTCCATGCTTCAGATGTCTAGGCCATACACCTTAGTGTGAGGGGGTTTGATGAAACTCATATCAGTTAGAGATACGGCCAATGTAGTCTTTAATGCTTGGGCAATCCACCCATTGAGCTAGCTTTTGGGGTTGAGTTGGGACTAAGAATCTCCAAGTTCCTATTTGTATTGCATCGAGTATATTAATTGCTAAGATGATTTGGTTCGTTGTTCAATAACCTAATAATTCAACTGTGATGCTTATGCGATCATGTTTCAGAGTTTATGTCGAGAGGAAGCTTATATGATTTTCTGCACAGACAAAAGGGTGTGTTTAAGCTTCCATCTTTACTAAAAGTAGCCATTGATGTTTCCAAGGGAATGAACTATTTGCACCAAAATAATATTATTCACAGGGACCTCAAGACTGCCAATCTTCTGATGGACGAAAATGAAGTAAGTAGACTCCATGAATGCACACATTGATACTTCCTGTTGCATTTTCATTGTTATTGGTCTATGTTTTTACTTCTGTTGGTAATATTTGGTTACAAATAAATCTATTAATCTTATTTCCTCAGTTGCAATCCTATATCACTAATGTATTGCCTATCTCGATTGTTGGGCCATTGCATTTTTTACTTTCCCATGTTCAATTCCTAGTTGATTATAGAATTTGTTAATCGATTGTGTGATAATTTACTACTAGAACTGTGGCTCTAATTCATTGATAAACCAAAACTGCAACTCATCTCGAATTAGATTAACTGCAGTTTTATAGTTTGTATAATTAAGTTTAAGATGAGCAGTTGCATAATAAGACCCAAAACATCTCAACATTTTATTTAATATGTACTCAAAGAGCATATCCTTCAACAGTTGGTCAAGGTTGCTGATTTTGGGGTTGCCAGAGTGCAAACTCAGTCTGGAGTGATGACAGCTGAAACTGGAACATACCGCTGGATGGCTCCTGAGGTATGATTTTTCTTGAACTTGATTTTATTCTTCTGCTTGATATTTTGGGTGATATCATTGTGCAAAGCGATTGGGTGATAGTTTGGATATTTTTCAGCAGTGGAAATCGTGTGTTTTTTTATTTTATTTTAAATGTATCAGGGGGGAAGACTTTGTGTGGGGAACAAGCTAAGTAAAATGCAAGTAAAACTGGTATTCAAACCGGAGTTCACAAAATATTGAAAAGTTTTTATCCTAATCTCTTAAATTAGGTTCTTGGTTTACCCTTAAAAATATTGTCCATGGTGAAGCAGAATTTAAAACTGGTAAAGAAAATAATATACTCGTTGCGATTTATGTTAATGTTTTTTTTATATGGATATTTGATTCACATTGTTTGGGCATTCAACTTACAGTTCAATAACAGTTTTATCCTTTTTTTTTTTTTTTTTTCTTTTCATAATTGAGGTTAAAAAAGATTTATTCTTTCTGGGCAATTTCCTTGCCAAGCATGTAATGCATTGTCGCATTATCAGAAAAAATGTAATGCATTGACACTTATTTTTTAGTTCAACTCACATTCATCAAGCATGCCTTGAAGTTTTAGAGTTTGGATTTCAAAGTGCTTATATGATAATGATATTTGGATTCCTTTTCTGTTTTTTTTCTTTCTTTCTTTCTTTAGCTGTTTGATATTGCTTCACATTTCTTTCCGTTGATTTGACTTGAAGGTTATTGAACACAAACCATATGACCAGAAGGCAGATGTTTTCAGTTTTGGAATAGCACTCTGGGAGCTTTTAACTGGAGAGGTGAGCATTTTTGTCTCAAGCAGCAAGCTTTGCTCTGCTTCATCTTTTTATTCGTTTCTTGTCTCAAACGTTGATCAGTCTTCTACCTGCTAATCCATTATATATTCTATTTTTTGTAACCAGCTGCCTTACTCTTACTTGACCCCATTACAAGCAGCAGTTGGTGTGGTGCAGAAGGTTTGTATTCTTTCCTCTTTGCGGATAATTTTATTGAAACATTTTTCTTGTTTACACACAATAACTCTATGTGGCTTAAATTGCTATTAGGGTCTACGGCCTACAATTCCCAAAAATACACATCCAAGAATTTCTGAACTACTACAGCGGTGCTGGCAACAAGATCCAAAGGAGAGACCAGCCTTTTCTGAAATAATTGAAATTCTCCAACACATAGCAAAAGAGGTAAACTATTAACATCATAAAAAAAAAAGAGGTTAAGTATTAAAGTTTTGAAAAATAGCACCAAGTTGTGCCTATTTAACAATTTTCTGTTTCATGTTTGTCAGGTTAATGATGTAGATCGACATAAAGATAAGTCATCACACGGTTTCCTCTCATCACTTAGGCGTGGCCATCACTGA
Protein sequence

>MS.gene063557.t1

EDSTQECCSVHWCLYSASKSLYCYUVYVERKLIUFSAQTKGCVUASIFTKSSHUCFQGNELFAPKUYYSQGPQDCQSSDGRKUIGQGCUFWGCQSANSVWSDDSUNWNIPLDGSUGYUTQTIUPEGRCFQFWNSTLGAFNWRAALLLLDPITSSSWCGAEGSTAYNSQKYTSKNFUTTTAVLATRSKGETSLFUNNUNSPTHSKRGUUCRSTURUVITRFPLITUAWPSL