Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063710.t1 | XP_013463184.1 | 79.6 | 186 | 34 | 2 | 1 | 185 | 1 | 183 | 8.70E-71 | 276.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063710.t1 | A0A072V5B6 | 79.6 | 186 | 34 | 2 | 1 | 185 | 1 | 183 | 6.2e-71 | 276.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052469 | MS.gene063710 | -0.813347 | 2.68E-51 | -1.69E-46 |
| MS.gene060717 | MS.gene063710 | -0.802229 | 6.19E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063710.t1 | MTR_2g436150 | 82.258 | 186 | 29 | 2 | 1 | 185 | 1 | 183 | 6.45e-106 | 301 |
| MS.gene063710.t1 | MTR_2g435810 | 82.258 | 186 | 29 | 2 | 1 | 185 | 1 | 183 | 6.45e-106 | 301 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063710.t1 | AT3G06840 | 51.020 | 196 | 76 | 7 | 1 | 185 | 1 | 187 | 1.73e-48 | 156 |
| MS.gene063710.t1 | AT5G49170 | 48.293 | 205 | 66 | 9 | 1 | 185 | 1 | 185 | 4.34e-44 | 145 |
Find 55 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTTCGGGCCACAATTTC+TGG | 0.043078 | 2.4:+55223922 | MS.gene063710:CDS |
| ATATACCTTACTTGAGTTTA+AGG | 0.160955 | 2.4:+55224061 | MS.gene063710:CDS |
| AGCTCGGGCTAAGGCCTTTC+GGG | 0.232026 | 2.4:+55223909 | MS.gene063710:CDS |
| CTTTCTTCTTCAAAAGGAAA+TGG | 0.274260 | 2.4:+55223833 | MS.gene063710:CDS |
| AAGCTCGGGCTAAGGCCTTT+CGG | 0.287446 | 2.4:+55223908 | MS.gene063710:CDS |
| CCTCAACATTTCAGTGACTA+TGG | 0.308915 | 2.4:+55223547 | MS.gene063710:CDS |
| CCTTTCGGGCCACAATTTCT+GGG | 0.320318 | 2.4:+55223923 | MS.gene063710:CDS |
| AGATTCACGTTTGTGCTTCA+TGG | 0.345486 | 2.4:-55223695 | None:intergenic |
| CGCCGGCGGCGTCAAAGTTC+CGG | 0.347476 | 2.4:-55224025 | None:intergenic |
| CTTGAAGTGTATGGAATCTA+TGG | 0.354127 | 2.4:-55223725 | None:intergenic |
| ATTTAAGTAACAAAGTAAAT+AGG | 0.355099 | 2.4:-55224132 | None:intergenic |
| GTGGGTCCACTACTCCTTAC+AGG | 0.363703 | 2.4:+55223971 | MS.gene063710:CDS |
| GAAATGTTGAGGGTCTGATA+TGG | 0.391377 | 2.4:-55223536 | None:intergenic |
| AATGCTCTTTCTTCTTCAAA+AGG | 0.392843 | 2.4:+55223827 | MS.gene063710:CDS |
| TATACCTTACTTGAGTTTAA+GGG | 0.394223 | 2.4:+55224062 | MS.gene063710:CDS |
| TTTCTTCTTCAAAAGGAAAT+GGG | 0.408788 | 2.4:+55223834 | MS.gene063710:CDS |
| CCTGAAAATAGTTGATTTGT+GGG | 0.429495 | 2.4:-55223576 | None:intergenic |
| ACCTGAAAATAGTTGATTTG+TGG | 0.442721 | 2.4:-55223577 | None:intergenic |
| CTACTCTCAGTCAAGTAAAC+AGG | 0.449601 | 2.4:-55223946 | None:intergenic |
| CAAAGTTCCGGCGAGTGGTC+CGG | 0.450141 | 2.4:-55224013 | None:intergenic |
| ACTTTGACGCCGCCGGCGAT+CGG | 0.485125 | 2.4:+55224030 | MS.gene063710:CDS |
| CGCCGGAACTTTGACGCCGC+CGG | 0.485779 | 2.4:+55224023 | MS.gene063710:CDS |
| CCGGCGAGTGGTCCGGAGGA+AGG | 0.503710 | 2.4:-55224006 | None:intergenic |
| AGCTCCCTTAAACTCAAGTA+AGG | 0.506363 | 2.4:-55224066 | None:intergenic |
| CCTCTGTTGCTGCTGTTCCA+TGG | 0.519512 | 2.4:-55224091 | None:intergenic |
| GCCGCCGGCGATCGGTGCGG+TGG | 0.523260 | 2.4:+55224038 | MS.gene063710:CDS |
| CCCAGAAATTGTGGCCCGAA+AGG | 0.523869 | 2.4:-55223923 | None:intergenic |
| CAATGATATTCATCAAGCTC+GGG | 0.527570 | 2.4:+55223894 | MS.gene063710:CDS |
| ACAATGATATTCATCAAGCT+CGG | 0.528614 | 2.4:+55223893 | MS.gene063710:CDS |
| GGCCTACTAGTAGTCCTGTA+AGG | 0.538444 | 2.4:-55223985 | None:intergenic |
| CATAGTCACTGAAATGTTGA+GGG | 0.543224 | 2.4:-55223546 | None:intergenic |
| CTCCTTACAGGACTACTAGT+AGG | 0.544863 | 2.4:+55223983 | MS.gene063710:CDS |
| TACTTGACTGAGAGTAGAAG+TGG | 0.545520 | 2.4:+55223952 | MS.gene063710:CDS |
| CCATAGTCACTGAAATGTTG+AGG | 0.547303 | 2.4:-55223547 | None:intergenic |
| AAATGTTGAGGGTCTGATAT+GGG | 0.548060 | 2.4:-55223535 | None:intergenic |
| GTAAACAGGCCCAGAAATTG+TGG | 0.553194 | 2.4:-55223932 | None:intergenic |
| CCTTCCTCCGGACCACTCGC+CGG | 0.558888 | 2.4:+55224006 | MS.gene063710:CDS |
| TATTCATCAAGCTCGGGCTA+AGG | 0.561484 | 2.4:+55223900 | MS.gene063710:CDS |
| CAATTTCAAAAGAAGAATCA+AGG | 0.563731 | 2.4:+55223759 | MS.gene063710:CDS |
| GGCGTCAAAGTTCCGGCGAG+TGG | 0.566894 | 2.4:-55224018 | None:intergenic |
| ATATCCACCGCACCGATCGC+CGG | 0.567737 | 2.4:-55224042 | None:intergenic |
| AGTAGTCCTGTAAGGAGTAG+TGG | 0.574794 | 2.4:-55223977 | None:intergenic |
| GAGTTTAAGGGAGCTTACCA+TGG | 0.580907 | 2.4:+55224074 | MS.gene063710:CDS |
| AATCAAAGAAGAAACGTTGG+TGG | 0.590435 | 2.4:+55223801 | MS.gene063710:CDS |
| GATGATCGTTACAATGATGA+TGG | 0.595872 | 2.4:+55223868 | MS.gene063710:CDS |
| ACTACTAGTAGGCCTTCCTC+CGG | 0.614378 | 2.4:+55223994 | MS.gene063710:CDS |
| TCCACCGCACCGATCGCCGG+CGG | 0.616643 | 2.4:-55224039 | None:intergenic |
| CCATGGAACAGCAGCAACAG+AGG | 0.617936 | 2.4:+55224091 | MS.gene063710:CDS |
| CAAAATCAAAGAAGAAACGT+TGG | 0.619265 | 2.4:+55223798 | MS.gene063710:CDS |
| AGTTCCGGCGAGTGGTCCGG+AGG | 0.639091 | 2.4:-55224010 | None:intergenic |
| GACGCCGCCGGCGATCGGTG+CGG | 0.647895 | 2.4:+55224035 | MS.gene063710:CDS |
| GCAACAGAGGATGTCTACCT+CGG | 0.665726 | 2.4:+55224104 | MS.gene063710:CDS |
| AAAGTAAATAGGCATAGCCG+AGG | 0.668649 | 2.4:-55224121 | None:intergenic |
| CATCATTGTAACGATCATCA+CGG | 0.676457 | 2.4:-55223864 | None:intergenic |
| ACTTGACTGAGAGTAGAAGT+GGG | 0.700833 | 2.4:+55223953 | MS.gene063710:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTCTATTTTCAAAACC+AGG | + | chr2.4:55223660-55223679 | MS.gene063710:intron | 20.0% |
| !!! | TTGATTCTTCTTTTGAAATT+GGG | - | chr2.4:55223760-55223779 | None:intergenic | 20.0% |
| ! | AATTAGAGAAAGAGAGTTAA+AGG | - | chr2.4:55223605-55223624 | None:intergenic | 25.0% |
| ! | ATATACCTTACTTGAGTTTA+AGG | + | chr2.4:55224061-55224080 | MS.gene063710:CDS | 25.0% |
| ! | ATTAGAGAAAGAGAGTTAAA+GGG | - | chr2.4:55223604-55223623 | None:intergenic | 25.0% |
| ! | CAATTTCAAAAGAAGAATCA+AGG | + | chr2.4:55223759-55223778 | MS.gene063710:CDS | 25.0% |
| ! | TATACCTTACTTGAGTTTAA+GGG | + | chr2.4:55224062-55224081 | MS.gene063710:CDS | 25.0% |
| ! | TTTCTTCTTCAAAAGGAAAT+GGG | + | chr2.4:55223834-55223853 | MS.gene063710:CDS | 25.0% |
| !!! | CTTGATTCTTCTTTTGAAAT+TGG | - | chr2.4:55223761-55223780 | None:intergenic | 25.0% |
| !!! | GTTTCTTCTTTGATTTTGTA+TGG | - | chr2.4:55223796-55223815 | None:intergenic | 25.0% |
| AATGCTCTTTCTTCTTCAAA+AGG | + | chr2.4:55223827-55223846 | MS.gene063710:CDS | 30.0% | |
| ACAATGATATTCATCAAGCT+CGG | + | chr2.4:55223893-55223912 | MS.gene063710:CDS | 30.0% | |
| CAAAATCAAAGAAGAAACGT+TGG | + | chr2.4:55223798-55223817 | MS.gene063710:CDS | 30.0% | |
| CTTTCTTCTTCAAAAGGAAA+TGG | + | chr2.4:55223833-55223852 | MS.gene063710:CDS | 30.0% | |
| ! | TTTTTGTAGCTTGAAGTGTA+TGG | - | chr2.4:55223737-55223756 | None:intergenic | 30.0% |
| !! | ACCTGAAAATAGTTGATTTG+TGG | - | chr2.4:55223580-55223599 | None:intergenic | 30.0% |
| !! | CCTGAAAATAGTTGATTTGT+GGG | - | chr2.4:55223579-55223598 | None:intergenic | 30.0% |
| AAATGTTGAGGGTCTGATAT+GGG | - | chr2.4:55223538-55223557 | None:intergenic | 35.0% | |
| AATCAAAGAAGAAACGTTGG+TGG | + | chr2.4:55223801-55223820 | MS.gene063710:CDS | 35.0% | |
| CAATGATATTCATCAAGCTC+GGG | + | chr2.4:55223894-55223913 | MS.gene063710:CDS | 35.0% | |
| CATAGTCACTGAAATGTTGA+GGG | - | chr2.4:55223549-55223568 | None:intergenic | 35.0% | |
| CATCATTGTAACGATCATCA+CGG | - | chr2.4:55223867-55223886 | None:intergenic | 35.0% | |
| CTTGAAGTGTATGGAATCTA+TGG | - | chr2.4:55223728-55223747 | None:intergenic | 35.0% | |
| GATGATCGTTACAATGATGA+TGG | + | chr2.4:55223868-55223887 | MS.gene063710:CDS | 35.0% | |
| ! | CCCACAAATCAACTATTTTC+AGG | + | chr2.4:55223576-55223595 | MS.gene063710:CDS | 35.0% |
| AAAGTAAATAGGCATAGCCG+AGG | - | chr2.4:55224124-55224143 | None:intergenic | 40.0% | |
| ACTTGACTGAGAGTAGAAGT+GGG | + | chr2.4:55223953-55223972 | MS.gene063710:CDS | 40.0% | |
| AGATTCACGTTTGTGCTTCA+TGG | - | chr2.4:55223698-55223717 | None:intergenic | 40.0% | |
| AGCTCCCTTAAACTCAAGTA+AGG | - | chr2.4:55224069-55224088 | None:intergenic | 40.0% | |
| CCATAGTCACTGAAATGTTG+AGG | - | chr2.4:55223550-55223569 | None:intergenic | 40.0% | |
| CCTCAACATTTCAGTGACTA+TGG | + | chr2.4:55223547-55223566 | MS.gene063710:CDS | 40.0% | |
| CTACTCTCAGTCAAGTAAAC+AGG | - | chr2.4:55223949-55223968 | None:intergenic | 40.0% | |
| GAAATGTTGAGGGTCTGATA+TGG | - | chr2.4:55223539-55223558 | None:intergenic | 40.0% | |
| GGGTCTGATATGGGAAAAAT+TGG | - | chr2.4:55223529-55223548 | None:intergenic | 40.0% | |
| TACTTGACTGAGAGTAGAAG+TGG | + | chr2.4:55223952-55223971 | MS.gene063710:CDS | 40.0% | |
| TCATGGCTTCTTCTAATACC+TGG | - | chr2.4:55223681-55223700 | None:intergenic | 40.0% | |
| AGTAGTCCTGTAAGGAGTAG+TGG | - | chr2.4:55223980-55223999 | None:intergenic | 45.0% | |
| CTCCTTACAGGACTACTAGT+AGG | + | chr2.4:55223983-55224002 | MS.gene063710:CDS | 45.0% | |
| GAGTTTAAGGGAGCTTACCA+TGG | + | chr2.4:55224074-55224093 | MS.gene063710:CDS | 45.0% | |
| GTAAACAGGCCCAGAAATTG+TGG | - | chr2.4:55223935-55223954 | None:intergenic | 45.0% | |
| TATTCATCAAGCTCGGGCTA+AGG | + | chr2.4:55223900-55223919 | MS.gene063710:CDS | 45.0% | |
| ACTACTAGTAGGCCTTCCTC+CGG | + | chr2.4:55223994-55224013 | MS.gene063710:CDS | 50.0% | |
| CCTTTCGGGCCACAATTTCT+GGG | + | chr2.4:55223923-55223942 | MS.gene063710:CDS | 50.0% | |
| GCAACAGAGGATGTCTACCT+CGG | + | chr2.4:55224104-55224123 | MS.gene063710:CDS | 50.0% | |
| GGCCTACTAGTAGTCCTGTA+AGG | - | chr2.4:55223988-55224007 | None:intergenic | 50.0% | |
| AAGCTCGGGCTAAGGCCTTT+CGG | + | chr2.4:55223908-55223927 | MS.gene063710:CDS | 55.0% | |
| CCATGGAACAGCAGCAACAG+AGG | + | chr2.4:55224091-55224110 | MS.gene063710:CDS | 55.0% | |
| CCCAGAAATTGTGGCCCGAA+AGG | - | chr2.4:55223926-55223945 | None:intergenic | 55.0% | |
| CCTCTGTTGCTGCTGTTCCA+TGG | - | chr2.4:55224094-55224113 | None:intergenic | 55.0% | |
| GCCTTTCGGGCCACAATTTC+TGG | + | chr2.4:55223922-55223941 | MS.gene063710:CDS | 55.0% | |
| GTGGGTCCACTACTCCTTAC+AGG | + | chr2.4:55223971-55223990 | MS.gene063710:CDS | 55.0% | |
| AGCTCGGGCTAAGGCCTTTC+GGG | + | chr2.4:55223909-55223928 | MS.gene063710:CDS | 60.0% | |
| CAAAGTTCCGGCGAGTGGTC+CGG | - | chr2.4:55224016-55224035 | None:intergenic | 60.0% | |
| ! | ATATCCACCGCACCGATCGC+CGG | - | chr2.4:55224045-55224064 | None:intergenic | 60.0% |
| ACTTTGACGCCGCCGGCGAT+CGG | + | chr2.4:55224030-55224049 | MS.gene063710:CDS | 65.0% | |
| GGCGTCAAAGTTCCGGCGAG+TGG | - | chr2.4:55224021-55224040 | None:intergenic | 65.0% | |
| AGTTCCGGCGAGTGGTCCGG+AGG | - | chr2.4:55224013-55224032 | None:intergenic | 70.0% | |
| CCTTCCTCCGGACCACTCGC+CGG | + | chr2.4:55224006-55224025 | MS.gene063710:CDS | 70.0% | |
| CGCCGGAACTTTGACGCCGC+CGG | + | chr2.4:55224023-55224042 | MS.gene063710:CDS | 70.0% | |
| CGCCGGCGGCGTCAAAGTTC+CGG | - | chr2.4:55224028-55224047 | None:intergenic | 70.0% | |
| CCGGCGAGTGGTCCGGAGGA+AGG | - | chr2.4:55224009-55224028 | None:intergenic | 75.0% | |
| ! | TCCACCGCACCGATCGCCGG+CGG | - | chr2.4:55224042-55224061 | None:intergenic | 75.0% |
| ! | GACGCCGCCGGCGATCGGTG+CGG | + | chr2.4:55224035-55224054 | MS.gene063710:CDS | 80.0% |
| ! | GCCGCCGGCGATCGGTGCGG+TGG | + | chr2.4:55224038-55224057 | MS.gene063710:CDS | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 55223511 | 55224152 | 55223511 | ID=MS.gene063710 |
| chr2.4 | mRNA | 55223511 | 55224152 | 55223511 | ID=MS.gene063710.t1;Parent=MS.gene063710 |
| chr2.4 | exon | 55223511 | 55223597 | 55223511 | ID=MS.gene063710.t1.exon1;Parent=MS.gene063710.t1 |
| chr2.4 | CDS | 55223511 | 55223597 | 55223511 | ID=cds.MS.gene063710.t1;Parent=MS.gene063710.t1 |
| chr2.4 | exon | 55223682 | 55224152 | 55223682 | ID=MS.gene063710.t1.exon2;Parent=MS.gene063710.t1 |
| chr2.4 | CDS | 55223682 | 55224152 | 55223682 | ID=cds.MS.gene063710.t1;Parent=MS.gene063710.t1 |
| Gene Sequence |
| Protein sequence |
