Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060717.t1 | XP_013467320.1 | 99.6 | 254 | 1 | 0 | 1 | 254 | 153 | 406 | 9.20E-140 | 506.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060717.t1 | Q03441 | 22.5 | 222 | 163 | 2 | 31 | 247 | 205 | 422 | 6.9e-11 | 69.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060717.t1 | A0A072VHW2 | 99.6 | 254 | 1 | 0 | 1 | 254 | 153 | 406 | 6.6e-140 | 506.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene060717 | 0.806357 | 8.56E-50 | -1.69E-46 |
| MS.gene050060 | MS.gene060717 | 0.830683 | 2.56E-55 | -1.69E-46 |
| MS.gene050209 | MS.gene060717 | 0.802551 | 5.32E-49 | -1.69E-46 |
| MS.gene052469 | MS.gene060717 | 0.869192 | 3.72E-66 | -1.69E-46 |
| MS.gene054810 | MS.gene060717 | -0.805816 | 1.11E-49 | -1.69E-46 |
| MS.gene059717 | MS.gene060717 | 0.836278 | 1.03E-56 | -1.69E-46 |
| MS.gene060717 | MS.gene061231 | 0.808475 | 3.04E-50 | -1.69E-46 |
| MS.gene060717 | MS.gene06260 | 0.81269 | 3.73E-51 | -1.69E-46 |
| MS.gene060717 | MS.gene063368 | 0.829797 | 4.21E-55 | -1.69E-46 |
| MS.gene060717 | MS.gene063710 | -0.802229 | 6.19E-49 | -1.69E-46 |
| MS.gene060717 | MS.gene065074 | -0.809033 | 2.31E-50 | -1.69E-46 |
| MS.gene060717 | MS.gene065846 | 0.823451 | 1.38E-53 | -1.69E-46 |
| MS.gene060717 | MS.gene067925 | 0.82904 | 6.43E-55 | -1.69E-46 |
| MS.gene060717 | MS.gene069869 | -0.824412 | 8.18E-54 | -1.69E-46 |
| MS.gene060717 | MS.gene071223 | 0.830728 | 2.49E-55 | -1.69E-46 |
| MS.gene060717 | MS.gene072070 | 0.810616 | 1.05E-50 | -1.69E-46 |
| MS.gene060717 | MS.gene073579 | 0.819983 | 8.72E-53 | -1.69E-46 |
| MS.gene060717 | MS.gene08083 | 0.812005 | 5.27E-51 | -1.69E-46 |
| MS.gene060717 | MS.gene20812 | 0.840177 | 1.02E-57 | -1.69E-46 |
| MS.gene060717 | MS.gene21013 | 0.810636 | 1.04E-50 | -1.69E-46 |
| MS.gene060717 | MS.gene21094 | 0.804253 | 2.36E-49 | -1.69E-46 |
| MS.gene060717 | MS.gene21238 | 0.803563 | 3.28E-49 | -1.69E-46 |
| MS.gene060717 | MS.gene25929 | 0.817069 | 3.99E-52 | -1.69E-46 |
| MS.gene060717 | MS.gene26624 | -0.81789 | 2.61E-52 | -1.69E-46 |
| MS.gene060717 | MS.gene26705 | -0.810741 | 9.91E-51 | -1.69E-46 |
| MS.gene060717 | MS.gene26817 | 0.807865 | 4.10E-50 | -1.69E-46 |
| MS.gene060717 | MS.gene30901 | 0.802467 | 5.53E-49 | -1.69E-46 |
| MS.gene060717 | MS.gene32793 | 0.803153 | 3.99E-49 | -1.69E-46 |
| MS.gene060717 | MS.gene36260 | 0.872136 | 4.01E-67 | -1.69E-46 |
| MS.gene060717 | MS.gene38024 | 0.824926 | 6.19E-54 | -1.69E-46 |
| MS.gene060717 | MS.gene38232 | 0.832613 | 8.56E-56 | -1.69E-46 |
| MS.gene060717 | MS.gene41649 | 0.811463 | 6.91E-51 | -1.69E-46 |
| MS.gene060717 | MS.gene48823 | 0.805453 | 1.33E-49 | -1.69E-46 |
| MS.gene060717 | MS.gene49436 | 0.814049 | 1.88E-51 | -1.69E-46 |
| MS.gene060717 | MS.gene51034 | 0.824038 | 1.00E-53 | -1.69E-46 |
| MS.gene060717 | MS.gene54795 | 0.819418 | 1.17E-52 | -1.69E-46 |
| MS.gene060717 | MS.gene59760 | 0.80835 | 3.23E-50 | -1.69E-46 |
| MS.gene060717 | MS.gene60931 | 0.802397 | 5.72E-49 | -1.69E-46 |
| MS.gene060717 | MS.gene61373 | 0.842704 | 2.20E-58 | -1.69E-46 |
| MS.gene060717 | MS.gene61544 | 0.859125 | 5.13E-63 | -1.69E-46 |
| MS.gene060717 | MS.gene68597 | 0.805263 | 1.45E-49 | -1.69E-46 |
| MS.gene060717 | MS.gene71924 | -0.800403 | 1.46E-48 | -1.69E-46 |
| MS.gene060717 | MS.gene74253 | -0.828901 | 6.95E-55 | -1.69E-46 |
| MS.gene060717 | MS.gene76250 | -0.800375 | 1.48E-48 | -1.69E-46 |
| MS.gene060717 | MS.gene76534 | -0.813641 | 2.31E-51 | -1.69E-46 |
| MS.gene060717 | MS.gene81254 | 0.840112 | 1.06E-57 | -1.69E-46 |
| MS.gene060717 | MS.gene85150 | -0.823927 | 1.06E-53 | -1.69E-46 |
| MS.gene060717 | MS.gene86339 | 0.848882 | 4.62E-60 | -1.69E-46 |
| MS.gene060717 | MS.gene89074 | 0.838108 | 3.50E-57 | -1.69E-46 |
| MS.gene060717 | MS.gene90899 | -0.832679 | 8.24E-56 | -1.69E-46 |
| MS.gene060717 | MS.gene94034 | 0.803645 | 3.16E-49 | -1.69E-46 |
| MS.gene060717 | MS.gene94217 | 0.822136 | 2.78E-53 | -1.69E-46 |
| MS.gene060717 | MS.gene98609 | 0.817394 | 3.37E-52 | -1.69E-46 |
| MS.gene060717 | MS.gene99889 | -0.812268 | 4.62E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060717.t1 | MTR_1g049180 | 99.606 | 254 | 1 | 0 | 1 | 254 | 153 | 406 | 0.0 | 525 |
| MS.gene060717.t1 | MTR_5g043180 | 66.800 | 250 | 81 | 2 | 2 | 250 | 160 | 408 | 4.96e-116 | 338 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060717.t1 | AT5G13610 | 75.600 | 250 | 61 | 0 | 1 | 250 | 152 | 401 | 1.10e-137 | 393 |
| MS.gene060717.t1 | AT1G69380 | 61.753 | 251 | 95 | 1 | 2 | 251 | 119 | 369 | 1.16e-108 | 318 |
Find 50 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACCATCCTTTCTCATTTC+AGG | 0.181892 | 1.3:-31663485 | None:intergenic |
| CTCCCATATCTGACCATATT+TGG | 0.221053 | 1.3:-31669113 | None:intergenic |
| ACCATATTTGGCATCTTTCC+AGG | 0.288418 | 1.3:-31669101 | None:intergenic |
| CCTGGTTCGTCTTTCTTGAA+TGG | 0.298986 | 1.3:+31663344 | MS.gene060717:CDS |
| CCACTTGTATTCTAGGTTGA+TGG | 0.321901 | 1.3:+31668122 | MS.gene060717:intron |
| AATTATGTAGTTCTCAAGTT+TGG | 0.333870 | 1.3:+31662220 | MS.gene060717:CDS |
| GTTGATGGAATTCGAACTAT+TGG | 0.339072 | 1.3:+31667973 | MS.gene060717:CDS |
| TACAAGAAATTCTTCAAAAC+AGG | 0.343403 | 1.3:+31669330 | MS.gene060717:CDS |
| ATGCCAAATATGGTCAGATA+TGG | 0.351572 | 1.3:+31669110 | MS.gene060717:CDS |
| AGTATTGCTCTTGATTACTA+TGG | 0.353838 | 1.3:+31668012 | MS.gene060717:CDS |
| ATGGCTATTTGAAAATTGTT+AGG | 0.362493 | 1.3:+31663441 | MS.gene060717:CDS |
| GTTCTCAAGTTTGGCAATCT+TGG | 0.366939 | 1.3:+31662229 | MS.gene060717:CDS |
| CGAACTATTGGTAGTGTACT+AGG | 0.382786 | 1.3:+31667985 | MS.gene060717:CDS |
| GTCAGATATGGGAGTATCTC+AGG | 0.391860 | 1.3:+31669122 | MS.gene060717:CDS |
| GCCTGGAAAGATGCCAAATA+TGG | 0.392620 | 1.3:+31669100 | MS.gene060717:CDS |
| TGCATCCATGTGCTCAAAGC+TGG | 0.404184 | 1.3:-31667913 | None:intergenic |
| TTACATAATGTTACAATACT+TGG | 0.404564 | 1.3:+31667948 | MS.gene060717:CDS |
| AATTTGCCTTGCCCACCAAC+TGG | 0.414032 | 1.3:-31668223 | None:intergenic |
| TGGCTCATTATTGCATTGAT+TGG | 0.416590 | 1.3:+31669371 | MS.gene060717:CDS |
| TTACAATACTTGGATGTTGA+TGG | 0.418643 | 1.3:+31667958 | MS.gene060717:CDS |
| CACATGGATGCAAGGAGGAC+TGG | 0.427102 | 1.3:+31667924 | MS.gene060717:CDS |
| TGATTTCAAGTTGAAATTTG+TGG | 0.429980 | 1.3:+31669180 | MS.gene060717:CDS |
| ATTGTTAGGAAGCATGCATC+TGG | 0.438001 | 1.3:+31663455 | MS.gene060717:CDS |
| TCTTTCTTGAATGGAACAAA+TGG | 0.446687 | 1.3:+31663353 | MS.gene060717:CDS |
| GTCCTCGAGCACGAGGTTGA+TGG | 0.465223 | 1.3:+31663422 | MS.gene060717:CDS |
| TTTGCTTCCTGAAATGAGAA+AGG | 0.519357 | 1.3:+31663478 | MS.gene060717:CDS |
| TTTGCAGATCAGATATCGCC+TGG | 0.524165 | 1.3:+31669083 | MS.gene060717:intron |
| TGCCAAATATGGTCAGATAT+GGG | 0.539187 | 1.3:+31669111 | MS.gene060717:CDS |
| TTGCTCTTGATTACTATGGC+CGG | 0.547854 | 1.3:+31668016 | MS.gene060717:CDS |
| TGGCAATCTTGGCGATTCCA+AGG | 0.548774 | 1.3:+31662240 | MS.gene060717:CDS |
| CTGGAAAATTTAAGATGCAA+AGG | 0.558742 | 1.3:+31668189 | MS.gene060717:CDS |
| TGTATTCTAGGTTGATGGAA+TGG | 0.567013 | 1.3:+31668127 | MS.gene060717:intron |
| CTCTATATGACATTGTTCAA+AGG | 0.569742 | 1.3:+31669411 | MS.gene060717:CDS |
| GCTTTGAGCACATGGATGCA+AGG | 0.583404 | 1.3:+31667916 | MS.gene060717:CDS |
| TCTTGATTACTATGGCCGGC+AGG | 0.586842 | 1.3:+31668020 | MS.gene060717:CDS |
| AATCGTGAGATGGAAGCGAC+TGG | 0.588881 | 1.3:+31668170 | MS.gene060717:CDS |
| GGAAATCTGATTTCCTTGAG+TGG | 0.592677 | 1.3:+31669351 | MS.gene060717:CDS |
| ATGCAATAATGAGCCACTCA+AGG | 0.594797 | 1.3:-31669364 | None:intergenic |
| GCTGATGTGATTCTAAAGCT+CGG | 0.595619 | 1.3:+31668254 | MS.gene060717:CDS |
| TTTCAAGTTGAAATTTGTGG+AGG | 0.601208 | 1.3:+31669183 | MS.gene060717:CDS |
| CCATTCAAGAAAGACGAACC+AGG | 0.605038 | 1.3:-31663344 | None:intergenic |
| TACAGACATAAATCGTGAGA+TGG | 0.607409 | 1.3:+31668160 | MS.gene060717:CDS |
| AGCCATCAACCTCGTGCTCG+AGG | 0.613037 | 1.3:-31663424 | None:intergenic |
| TGGAACAAATGGCTGTTACA+TGG | 0.615782 | 1.3:+31663364 | MS.gene060717:CDS |
| CTTCCTGAAATGAGAAAGGA+TGG | 0.617434 | 1.3:+31663482 | MS.gene060717:CDS |
| TAAAGCTCGGACTATTTGAG+AGG | 0.622265 | 1.3:+31668267 | MS.gene060717:CDS |
| GAGGACATTAAACAATACAA+TGG | 0.625297 | 1.3:-31663405 | None:intergenic |
| GTTTAATGTCCTCGAGCACG+AGG | 0.633334 | 1.3:+31663415 | MS.gene060717:CDS |
| TCAGATATGGGAGTATCTCA+GGG | 0.654645 | 1.3:+31669123 | MS.gene060717:CDS |
| TTGAGCACATGGATGCAAGG+AGG | 0.721239 | 1.3:+31667919 | MS.gene060717:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 31662214 | 31669451 | 31662214 | ID=MS.gene060717 |
| chr1.3 | mRNA | 31662214 | 31669451 | 31662214 | ID=MS.gene060717.t1;Parent=MS.gene060717 |
| chr1.3 | exon | 31662214 | 31662261 | 31662214 | ID=MS.gene060717.t1.exon1;Parent=MS.gene060717.t1 |
| chr1.3 | CDS | 31662214 | 31662261 | 31662214 | ID=cds.MS.gene060717.t1;Parent=MS.gene060717.t1 |
| chr1.3 | exon | 31663341 | 31663503 | 31663341 | ID=MS.gene060717.t1.exon2;Parent=MS.gene060717.t1 |
| chr1.3 | CDS | 31663341 | 31663503 | 31663341 | ID=cds.MS.gene060717.t1;Parent=MS.gene060717.t1 |
| chr1.3 | exon | 31667893 | 31668041 | 31667893 | ID=MS.gene060717.t1.exon3;Parent=MS.gene060717.t1 |
| chr1.3 | CDS | 31667893 | 31668041 | 31667893 | ID=cds.MS.gene060717.t1;Parent=MS.gene060717.t1 |
| chr1.3 | exon | 31668137 | 31668288 | 31668137 | ID=MS.gene060717.t1.exon4;Parent=MS.gene060717.t1 |
| chr1.3 | CDS | 31668137 | 31668288 | 31668137 | ID=cds.MS.gene060717.t1;Parent=MS.gene060717.t1 |
| chr1.3 | exon | 31669090 | 31669204 | 31669090 | ID=MS.gene060717.t1.exon5;Parent=MS.gene060717.t1 |
| chr1.3 | CDS | 31669090 | 31669204 | 31669090 | ID=cds.MS.gene060717.t1;Parent=MS.gene060717.t1 |
| chr1.3 | exon | 31669314 | 31669451 | 31669314 | ID=MS.gene060717.t1.exon6;Parent=MS.gene060717.t1 |
| chr1.3 | CDS | 31669314 | 31669451 | 31669314 | ID=cds.MS.gene060717.t1;Parent=MS.gene060717.t1 |
| Gene Sequence |
| Protein sequence |
