Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064184.t1 | XP_028762943.1 | 46 | 348 | 72 | 3 | 1 | 232 | 1 | 348 | 3.70E-71 | 278.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064184.t1 | A0A1U8PVE2 | 49.8 | 269 | 84 | 2 | 1 | 218 | 1 | 269 | 1.4e-64 | 255.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049531 | MS.gene064184 | 0.809488 | 1.85E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene064184 | 0.80456 | 2.04E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene064184 | 0.801961 | 7.03E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene064184 | 0.857992 | 1.12E-62 | -1.69E-46 |
| MS.gene059584 | MS.gene064184 | 0.811022 | 8.62E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064184.t1 | MTR_7g062720 | 59.740 | 154 | 56 | 1 | 1 | 154 | 1 | 148 | 2.34e-62 | 192 |
| MS.gene064184.t1 | MTR_5g061150 | 66.372 | 113 | 38 | 0 | 20 | 132 | 10 | 122 | 3.25e-47 | 153 |
| MS.gene064184.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 152 | 232 | 5 | 80 | 1.06e-23 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGCTCTTAATATTTC+TGG | 0.080746 | 4.2:-56208261 | MS.gene064184:CDS |
| TTCGGCATTTAAATGAATTT+TGG | 0.166319 | 4.2:+56208209 | None:intergenic |
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 4.2:-56207366 | MS.gene064184:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 4.2:-56207374 | MS.gene064184:CDS |
| ATGGTTATATTTGCGTTTAC+AGG | 0.259438 | 4.2:-56207986 | MS.gene064184:CDS |
| AAATTGTCCCAAAGATCTTT+TGG | 0.267031 | 4.2:+56208063 | None:intergenic |
| TATTTCTGGAAATAATTATC+TGG | 0.282933 | 4.2:-56208247 | MS.gene064184:CDS |
| TAGAAGATCCAAAAGATCTT+TGG | 0.283634 | 4.2:-56208071 | MS.gene064184:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 4.2:+56207212 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 4.2:+56207397 | None:intergenic |
| TGAAGATGGTTATGGTAATA+TGG | 0.347497 | 4.2:-56207314 | MS.gene064184:CDS |
| CTGGAAATAATTATCTGGCT+TGG | 0.347827 | 4.2:-56208242 | MS.gene064184:CDS |
| TGGAAATAATTATCTGGCTT+GGG | 0.353242 | 4.2:-56208241 | MS.gene064184:CDS |
| AACCATTCATTACGAGCATT+AGG | 0.386252 | 4.2:+56208003 | None:intergenic |
| TTTGGGACAATTTAAAAGAA+AGG | 0.395342 | 4.2:-56208053 | MS.gene064184:CDS |
| AGAAGATCCAAAAGATCTTT+GGG | 0.400056 | 4.2:-56208070 | MS.gene064184:CDS |
| TGCTATCGTTGTGGAGGAAA+AGG | 0.414829 | 4.2:-56207445 | MS.gene064184:CDS |
| CCTTCGTCACCACCTTCATA+AGG | 0.433246 | 4.2:-56208118 | MS.gene064184:CDS |
| ATTAACACTGTGTGGAGAAA+AGG | 0.440407 | 4.2:-56207911 | MS.gene064184:intron |
| CAAAACAACTGAACAACAAA+AGG | 0.454927 | 4.2:-56208157 | MS.gene064184:CDS |
| AATTGTATCTCCATGCCCTT+CGG | 0.460742 | 4.2:+56208191 | None:intergenic |
| AGAAATCAGCAGCATCTAAG+TGG | 0.464577 | 4.2:+56207284 | None:intergenic |
| AATGTTGTAAAGAATGATAT+TGG | 0.471241 | 4.2:-56207496 | MS.gene064184:CDS |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 4.2:-56207365 | MS.gene064184:CDS |
| CATTTAAATGCCGAAGGGCA+TGG | 0.494088 | 4.2:-56208201 | MS.gene064184:CDS |
| GAAAATGATGAAGATGGTTA+TGG | 0.498501 | 4.2:-56207322 | MS.gene064184:CDS |
| GTTGTGGAGGAAAAGGTCAT+TGG | 0.504176 | 4.2:-56207438 | MS.gene064184:CDS |
| TTCCTAATGCTCGTAATGAA+TGG | 0.506622 | 4.2:-56208005 | MS.gene064184:CDS |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 4.2:-56207230 | MS.gene064184:CDS |
| CATGGAGATACAATTAAAGA+AGG | 0.533844 | 4.2:-56208183 | MS.gene064184:CDS |
| AAAATTCATTTAAATGCCGA+AGG | 0.537172 | 4.2:-56208207 | MS.gene064184:CDS |
| GCATGCGAAAATGATGAAGA+TGG | 0.557414 | 4.2:-56207328 | MS.gene064184:CDS |
| GTCTGTAATCGAATATAACT+CGG | 0.574961 | 4.2:-56207956 | MS.gene064184:CDS |
| AACTGAACAACAAAAGGCTA+AGG | 0.582581 | 4.2:-56208151 | MS.gene064184:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 4.2:-56207454 | MS.gene064184:CDS |
| ATTCATTACGAGCATTAGGA+AGG | 0.589984 | 4.2:+56208007 | None:intergenic |
| CCTTATGAAGGTGGTGACGA+AGG | 0.598722 | 4.2:+56208118 | None:intergenic |
| AGTATTTGCTATCGTTGTGG+AGG | 0.599695 | 4.2:-56207451 | MS.gene064184:CDS |
| GAAATCAGCAGCATCTAAGT+GGG | 0.621669 | 4.2:+56207285 | None:intergenic |
| AAATTCATTTAAATGCCGAA+GGG | 0.631745 | 4.2:-56208206 | MS.gene064184:CDS |
| GTGGTGACGAAGGAATATCA+TGG | 0.663314 | 4.2:+56208128 | None:intergenic |
| ACTTCTCTATTAACACTGTG+TGG | 0.668278 | 4.2:-56207919 | MS.gene064184:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | - | chr4.2:56208014-56208033 | MS.gene064184:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | - | chr4.2:56208145-56208164 | MS.gene064184:CDS | 20.0% |
| !!! | TATTTCTGGAAATAATTATC+TGG | - | chr4.2:56207263-56207282 | MS.gene064184:CDS | 20.0% |
| ! | AAAATTCATTTAAATGCCGA+AGG | - | chr4.2:56207303-56207322 | MS.gene064184:CDS | 25.0% |
| ! | AAATTCATTTAAATGCCGAA+GGG | - | chr4.2:56207304-56207323 | MS.gene064184:CDS | 25.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | - | chr4.2:56208144-56208163 | MS.gene064184:CDS | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | + | chr4.2:56208116-56208135 | None:intergenic | 25.0% |
| !! | TATTTTCATCTCTAATGAGA+TGG | + | chr4.2:56208277-56208296 | None:intergenic | 25.0% |
| !! | TTACAACATTTTTCATCTTC+TGG | + | chr4.2:56208004-56208023 | None:intergenic | 25.0% |
| !! | TTCGGCATTTAAATGAATTT+TGG | + | chr4.2:56207304-56207323 | None:intergenic | 25.0% |
| !! | TTTGGGACAATTTAAAAGAA+AGG | - | chr4.2:56207457-56207476 | MS.gene064184:CDS | 25.0% |
| !!! | CATTTTTTAGATCCTTATGA+AGG | + | chr4.2:56207407-56207426 | None:intergenic | 25.0% |
| !!! | GAGAAAATGTGTTTTTGTTT+TGG | + | chr4.2:56207980-56207999 | None:intergenic | 25.0% |
| AGAAGATCCAAAAGATCTTT+GGG | - | chr4.2:56207440-56207459 | MS.gene064184:CDS | 30.0% | |
| ATGGTTATATTTGCGTTTAC+AGG | - | chr4.2:56207524-56207543 | MS.gene064184:intron | 30.0% | |
| CAAAACAACTGAACAACAAA+AGG | - | chr4.2:56207353-56207372 | MS.gene064184:CDS | 30.0% | |
| CATGGAGATACAATTAAAGA+AGG | - | chr4.2:56207327-56207346 | MS.gene064184:CDS | 30.0% | |
| CATTAGAGATGAAAATATCC+AGG | - | chr4.2:56208280-56208299 | MS.gene064184:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | - | chr4.2:56208136-56208155 | MS.gene064184:CDS | 30.0% | |
| GAAAATGATGAAGATGGTTA+TGG | - | chr4.2:56208188-56208207 | MS.gene064184:CDS | 30.0% | |
| GTCTGTAATCGAATATAACT+CGG | - | chr4.2:56207554-56207573 | MS.gene064184:intron | 30.0% | |
| TACATTAGATGCATGAAAAG+TGG | + | chr4.2:56207658-56207677 | None:intergenic | 30.0% | |
| TAGAAGATCCAAAAGATCTT+TGG | - | chr4.2:56207439-56207458 | MS.gene064184:CDS | 30.0% | |
| TGAAGATGGTTATGGTAATA+TGG | - | chr4.2:56208196-56208215 | MS.gene064184:CDS | 30.0% | |
| ! | AAATTGTCCCAAAGATCTTT+TGG | + | chr4.2:56207450-56207469 | None:intergenic | 30.0% |
| ! | AAGATCTTTTGGATCTTCTA+TGG | + | chr4.2:56207439-56207458 | None:intergenic | 30.0% |
| ! | GCTTTTGATGAAAAATCACT+CGG | - | chr4.2:56207764-56207783 | MS.gene064184:intron | 30.0% |
| ! | TGGAAATAATTATCTGGCTT+GGG | - | chr4.2:56207269-56207288 | MS.gene064184:CDS | 30.0% |
| ! | TTTGAGGCTCTTAATATTTC+TGG | - | chr4.2:56207249-56207268 | MS.gene064184:CDS | 30.0% |
| ! | TTTTTAGATCCTTATGAAGG+TGG | + | chr4.2:56207404-56207423 | None:intergenic | 30.0% |
| !! | GGTTTTAATCGTAATCACAA+TGG | - | chr4.2:56207930-56207949 | MS.gene064184:CDS | 30.0% |
| !!! | ACCTTTTAAAACCCTTTTCA+CGG | + | chr4.2:56207698-56207717 | None:intergenic | 30.0% |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | + | chr4.2:56208139-56208158 | None:intergenic | 30.0% |
| AACCATTCATTACGAGCATT+AGG | + | chr4.2:56207510-56207529 | None:intergenic | 35.0% | |
| AACTGAACAACAAAAGGCTA+AGG | - | chr4.2:56207359-56207378 | MS.gene064184:CDS | 35.0% | |
| ACTTCTCTATTAACACTGTG+TGG | - | chr4.2:56207591-56207610 | MS.gene064184:intron | 35.0% | |
| ATTAACACTGTGTGGAGAAA+AGG | - | chr4.2:56207599-56207618 | MS.gene064184:intron | 35.0% | |
| ATTCATTACGAGCATTAGGA+AGG | + | chr4.2:56207506-56207525 | None:intergenic | 35.0% | |
| CTGGAAATAATTATCTGGCT+TGG | - | chr4.2:56207268-56207287 | MS.gene064184:CDS | 35.0% | |
| TTCCTAATGCTCGTAATGAA+TGG | - | chr4.2:56207505-56207524 | MS.gene064184:CDS | 35.0% | |
| ! | AGATGGTCAATCTTTTCATC+AGG | + | chr4.2:56208260-56208279 | None:intergenic | 35.0% |
| ! | CCTCAAAATCAAGTTTTGCA+AGG | + | chr4.2:56207236-56207255 | None:intergenic | 35.0% |
| ! | CCTTGCAAAACTTGATTTTG+AGG | - | chr4.2:56207233-56207252 | MS.gene064184:CDS | 35.0% |
| ! | CTTTTGGATCTTCTATGGTA+AGG | + | chr4.2:56207434-56207453 | None:intergenic | 35.0% |
| !!! | ACCGTGAAAAGGGTTTTAAA+AGG | - | chr4.2:56207694-56207713 | MS.gene064184:intron | 35.0% |
| AATTGTATCTCCATGCCCTT+CGG | + | chr4.2:56207322-56207341 | None:intergenic | 40.0% | |
| AGAAATCAGCAGCATCTAAG+TGG | + | chr4.2:56208229-56208248 | None:intergenic | 40.0% | |
| CAGCAACAATACCGTGAAAA+GGG | - | chr4.2:56207684-56207703 | MS.gene064184:intron | 40.0% | |
| CTTAATATCATGCCTGCTTG+TGG | - | chr4.2:56207725-56207744 | MS.gene064184:intron | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | + | chr4.2:56208228-56208247 | None:intergenic | 40.0% | |
| GATCACTATGGACAAAATCG+TGG | - | chr4.2:56207837-56207856 | MS.gene064184:intron | 40.0% | |
| GCATGCGAAAATGATGAAGA+TGG | - | chr4.2:56208182-56208201 | MS.gene064184:CDS | 40.0% | |
| GTAGCAACACATGATCACTA+TGG | - | chr4.2:56207825-56207844 | MS.gene064184:intron | 40.0% | |
| ! | GAAGGATGACAGTTTTCTGA+TGG | + | chr4.2:56207488-56207507 | None:intergenic | 40.0% |
| ! | GTGTTGCTACATTCACTTCT+GGG | + | chr4.2:56207815-56207834 | None:intergenic | 40.0% |
| ! | TATTTTGTTCTGCCACAAGC+AGG | + | chr4.2:56207740-56207759 | None:intergenic | 40.0% |
| ! | TGTGTTGCTACATTCACTTC+TGG | + | chr4.2:56207816-56207835 | None:intergenic | 40.0% |
| !! | AGTATTTGCTATCGTTGTGG+AGG | - | chr4.2:56208059-56208078 | MS.gene064184:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | - | chr4.2:56208056-56208075 | MS.gene064184:CDS | 40.0% |
| CATTTAAATGCCGAAGGGCA+TGG | - | chr4.2:56207309-56207328 | MS.gene064184:CDS | 45.0% | |
| GCAGCAACAATACCGTGAAA+AGG | - | chr4.2:56207683-56207702 | MS.gene064184:intron | 45.0% | |
| GTTGTGGAGGAAAAGGTCAT+TGG | - | chr4.2:56208072-56208091 | MS.gene064184:CDS | 45.0% | |
| TATGGACAAAATCGTGGTCG+TGG | - | chr4.2:56207843-56207862 | MS.gene064184:intron | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | - | chr4.2:56208065-56208084 | MS.gene064184:CDS | 45.0% | |
| TTTCACGGTATTGTTGCTGC+AGG | + | chr4.2:56207683-56207702 | None:intergenic | 45.0% | |
| ! | GCTACATTCACTTCTGGGAA+TGG | + | chr4.2:56207810-56207829 | None:intergenic | 45.0% |
| ! | GTGGTGACGAAGGAATATCA+TGG | + | chr4.2:56207385-56207404 | None:intergenic | 45.0% |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | + | chr4.2:56208105-56208124 | None:intergenic | 45.0% |
| ACGTGGTCGTGGTTATGGAA+GGG | - | chr4.2:56207896-56207915 | MS.gene064184:intron | 50.0% | |
| CCTTCGTCACCACCTTCATA+AGG | - | chr4.2:56207392-56207411 | MS.gene064184:CDS | 50.0% | |
| ! | CCTTATGAAGGTGGTGACGA+AGG | + | chr4.2:56207395-56207414 | None:intergenic | 50.0% |
| ! | TATGGAAGGGGACGTGGTTT+TGG | - | chr4.2:56207909-56207928 | MS.gene064184:intron | 50.0% |
| CGTGGTCGTGGTTATGGAAG+GGG | - | chr4.2:56207897-56207916 | MS.gene064184:intron | 55.0% | |
| GACGTGGTCGTGGTTATGGA+AGG | - | chr4.2:56207895-56207914 | MS.gene064184:intron | 55.0% | |
| ! | GGGAATGGAGCACTACCAGT+GGG | + | chr4.2:56207795-56207814 | None:intergenic | 55.0% |
| ! | TGGGAATGGAGCACTACCAG+TGG | + | chr4.2:56207796-56207815 | None:intergenic | 55.0% |
| CAAAATCGTGGTCGTGGCCG+TGG | - | chr4.2:56207849-56207868 | MS.gene064184:intron | 60.0% | |
| CACGTCCACGACCATGACCA+CGG | + | chr4.2:56207869-56207888 | None:intergenic | 60.0% | |
| CGTGGACGTGGTCGTGGTTA+TGG | - | chr4.2:56207891-56207910 | MS.gene064184:intron | 60.0% | |
| CGTGGTTATGGAAGGGGACG+TGG | - | chr4.2:56207903-56207922 | MS.gene064184:intron | 60.0% | |
| ! | AATCACTCGGCTCGTCCCAC+TGG | - | chr4.2:56207777-56207796 | MS.gene064184:intron | 60.0% |
| CATGGTCGTGGACGTGGACG+TGG | - | chr4.2:56207873-56207892 | MS.gene064184:intron | 65.0% | |
| CGTGGTCATGGTCGTGGACG+TGG | - | chr4.2:56207867-56207886 | MS.gene064184:intron | 65.0% | |
| CGTGGACGTGGACGTGGACG+TGG | - | chr4.2:56207879-56207898 | MS.gene064184:intron | 70.0% | |
| CGTGGACGTGGACGTGGTCG+TGG | - | chr4.2:56207885-56207904 | MS.gene064184:intron | 70.0% | |
| CGTGGCCGTGGTCATGGTCG+TGG | - | chr4.2:56207861-56207880 | MS.gene064184:intron | 70.0% | |
| CGTGGTCGTGGCCGTGGTCA+TGG | - | chr4.2:56207855-56207874 | MS.gene064184:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 56207225 | 56208307 | 56207225 | ID=MS.gene064184 |
| chr4.2 | mRNA | 56207225 | 56208307 | 56207225 | ID=MS.gene064184.t1;Parent=MS.gene064184 |
| chr4.2 | exon | 56207912 | 56208307 | 56207912 | ID=MS.gene064184.t1.exon1;Parent=MS.gene064184.t1 |
| chr4.2 | CDS | 56207912 | 56208307 | 56207912 | ID=cds.MS.gene064184.t1;Parent=MS.gene064184.t1 |
| chr4.2 | exon | 56207225 | 56207527 | 56207225 | ID=MS.gene064184.t1.exon2;Parent=MS.gene064184.t1 |
| chr4.2 | CDS | 56207225 | 56207527 | 56207225 | ID=cds.MS.gene064184.t1;Parent=MS.gene064184.t1 |
| Gene Sequence |
| Protein sequence |
