AlfalfaGEDB | MS.gene049531

M. sativa cultivar XinJiangDaYe / MS.gene049531

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049531.t1	RHN67982.1	84.7	144	21	1	2	144	22	165	1.00E-58	236.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049531.t1	A0A396IQX0	84.7	144	21	1	2	144	22	165	7.3e-59	236.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049531	MS.gene055058	0.804081	2.56E-49	-1.69E-46
MS.gene049531	MS.gene058515	0.808584	2.88E-50	-1.69E-46
MS.gene049531	MS.gene059272	0.802106	6.57E-49	-1.69E-46
MS.gene049531	MS.gene064184	0.809488	1.85E-50	-1.69E-46
MS.gene049531	MS.gene068453	0.817009	4.12E-52	-1.69E-46
MS.gene049531	MS.gene072397	-0.804951	1.69E-49	-1.69E-46
MS.gene049531	MS.gene073139	0.803104	4.09E-49	-1.69E-46
MS.gene049531	MS.gene09348	0.804671	1.93E-49	-1.69E-46
MS.gene049531	MS.gene22267	0.804202	2.42E-49	-1.69E-46
MS.gene049531	MS.gene26062	0.801676	8.05E-49	-1.69E-46
MS.gene049531	MS.gene31283	0.813365	2.66E-51	-1.69E-46
MS.gene049531	MS.gene38011	0.806855	6.72E-50	-1.69E-46
MS.gene049531	MS.gene41113	0.817524	3.15E-52	-1.69E-46
MS.gene049531	MS.gene41114	0.804394	2.21E-49	-1.69E-46
MS.gene049531	MS.gene43372	0.812005	5.27E-51	-1.69E-46
MS.gene049531	MS.gene51189	0.80277	4.79E-49	-1.69E-46
MS.gene049531	MS.gene53704	0.825588	4.32E-54	-1.69E-46
MS.gene049531	MS.gene58686	0.801679	8.04E-49	-1.69E-46
MS.gene049531	MS.gene61742	0.81679	4.61E-52	-1.69E-46
MS.gene049531	MS.gene64567	0.831018	2.12E-55	-1.69E-46
MS.gene049531	MS.gene66682	0.812117	4.98E-51	-1.69E-46
MS.gene049531	MS.gene67984	0.80586	1.09E-49	-1.69E-46
MS.gene049531	MS.gene68999	0.805232	1.47E-49	-1.69E-46
MS.gene049531	MS.gene69102	0.813267	2.79E-51	-1.69E-46
MS.gene049531	MS.gene69594	0.805391	1.37E-49	-1.69E-46
MS.gene049531	MS.gene71430	0.81247	4.17E-51	-1.69E-46
MS.gene049531	MS.gene78916	0.812518	4.07E-51	-1.69E-46
MS.gene049531	MS.gene82793	0.802633	5.11E-49	-1.69E-46
MS.gene049531	MS.gene82962	0.821998	3.00E-53	-1.69E-46
MS.gene049531	MS.gene85694	0.817888	2.61E-52	-1.69E-46
MS.gene049531	MS.gene98550	0.8035	3.38E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049531.t1	MTR_3g062400	78.472	144	30	1	2	144	134	277	1.56e-75	226
MS.gene049531.t1	MTR_3g451150	81.944	144	25	1	2	144	1721	1864	2.76e-71	232
MS.gene049531.t1	MTR_3g452250	81.944	144	25	1	2	144	1721	1864	2.76e-71	232
MS.gene049531.t1	MTR_3g062180	85.271	129	18	1	2	129	500	628	3.50e-70	222
MS.gene049531.t1	MTR_0922s0020	74.306	144	35	2	2	144	55	197	9.17e-70	209
MS.gene049531.t1	MTR_3g062420	82.963	135	22	1	11	144	1792	1926	1.10e-68	224
MS.gene049531.t1	MTR_3g062140	85.271	129	18	1	2	129	1618	1746	1.23e-67	221
MS.gene049531.t1	MTR_3g435040	78.322	143	30	1	3	144	1468	1610	4.49e-67	220
MS.gene049531.t1	MTR_3g435040	78.322	143	30	1	3	144	1466	1608	4.54e-67	220
MS.gene049531.t1	MTR_3g435040	78.322	143	30	1	3	144	1686	1828	5.48e-67	219
MS.gene049531.t1	MTR_3g435040	78.322	143	30	1	3	144	1683	1825	5.54e-67	219
MS.gene049531.t1	MTR_3g435040	78.322	143	30	1	3	144	1407	1549	5.55e-67	219
MS.gene049531.t1	MTR_2g049960	66.242	157	38	2	3	144	152	308	1.88e-63	197
MS.gene049531.t1	MTR_3g027360	75.000	116	28	1	2	116	163	278	8.84e-58	181
MS.gene049531.t1	MTR_3g062320	65.605	157	39	2	3	144	1442	1598	1.26e-57	193
MS.gene049531.t1	MTR_3g062340	66.879	157	37	2	3	144	1778	1934	1.82e-57	192
MS.gene049531.t1	MTR_3g466750	63.924	158	42	2	1	143	1695	1852	7.19e-57	191
MS.gene049531.t1	MTR_3g464920	66.667	150	42	2	3	144	1584	1733	4.19e-55	186
MS.gene049531.t1	MTR_1147s0010	66.207	145	39	3	1	144	22	157	1.29e-54	169
MS.gene049531.t1	MTR_3g063010	62.195	164	40	2	3	144	1631	1794	1.49e-54	184
MS.gene049531.t1	MTR_3g062010	64.138	145	37	2	2	131	1087	1231	1.76e-53	181
MS.gene049531.t1	MTR_3g464870	58.537	164	46	2	3	144	1706	1869	3.25e-51	174
MS.gene049531.t1	MTR_3g464950	58.896	163	45	2	3	143	1617	1779	3.44e-51	174
MS.gene049531.t1	MTR_3g062980	60.897	156	39	2	3	144	543	690	2.13e-50	171
MS.gene049531.t1	MTR_3g464810	69.600	125	37	1	3	126	1725	1849	4.94e-50	171
MS.gene049531.t1	MTR_3g464840	60.510	157	42	3	3	144	1562	1713	5.76e-50	171
MS.gene049531.t1	MTR_3g069220	73.563	87	23	0	3	89	43	129	2.95e-42	137
MS.gene049531.t1	MTR_7g026240	59.375	128	36	3	33	144	197	324	1.16e-40	139
MS.gene049531.t1	MTR_3g048690	65.254	118	16	2	2	119	1178	1270	2.11e-40	144
MS.gene049531.t1	MTR_2g451270	50.955	157	34	3	3	144	108	236	5.96e-40	134
MS.gene049531.t1	MTR_2g023000	57.724	123	28	2	2	123	311	410	2.89e-33	120
MS.gene049531.t1	MTR_3g062130	47.020	151	28	4	3	138	302	415	4.35e-30	112
MS.gene049531.t1	MTR_3g466550	56.579	76	18	2	84	144	22	97	2.63e-18	75.5
MS.gene049531.t1	MTR_2g450490	56.579	76	18	2	84	144	180	255	3.09e-17	75.9
MS.gene049531.t1	MTR_3g063320	56.923	65	13	2	95	144	60	124	6.53e-12	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 24 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCATATTTCAAGTACTTCT+TGG	0.215722	3.2:-57560255	None:intergenic
GTCTTCCTCTGTAGCTGTTT+TGG	0.251316	3.2:+57560616	MS.gene049531:CDS
CTAACACATCCAACACACTA+TGG	0.343716	3.2:-57560406	None:intergenic
CATCCAACACACTATGGGTT+TGG	0.445633	3.2:-57560400	None:intergenic
GCTTGAGAAACTTGTAAGCC+TGG	0.453653	3.2:-57560524	None:intergenic
TAGTTCAGTCAGGCAGTTGT+AGG	0.463660	3.2:-57560366	None:intergenic
AATGTGGATTTGATAATGAT+TGG	0.466807	3.2:+57560474	MS.gene049531:CDS
CATAGTGTGTTGGATGTGTT+AGG	0.467513	3.2:+57560407	MS.gene049531:CDS
GAAATTTCAATAGTTCAGTC+AGG	0.486190	3.2:-57560376	None:intergenic
TTTGATAATGATTGGTTGGA+TGG	0.487302	3.2:+57560482	MS.gene049531:CDS
GTTGAAAAGCGTGCTTTCCC+AGG	0.489166	3.2:+57560506	MS.gene049531:CDS
CTCACTGAGTAATAGATACA+AGG	0.493355	3.2:+57560337	MS.gene049531:CDS
TGGATTTGATAATGATTGGT+TGG	0.500907	3.2:+57560478	MS.gene049531:CDS
GTTTCCTTTAAAAGAACAGA+AGG	0.527263	3.2:-57560314	None:intergenic
ATACAAGATGTGCGCAAATG+TGG	0.550948	3.2:+57560458	MS.gene049531:CDS
TAACACATCCAACACACTAT+GGG	0.560461	3.2:-57560405	None:intergenic
CTTGAGAAACTTGTAAGCCT+GGG	0.564858	3.2:-57560523	None:intergenic
TCTCCAAACCCATAGTGTGT+TGG	0.584472	3.2:+57560397	MS.gene049531:CDS
GGAAACTATCATTCAAACAG+AGG	0.587359	3.2:+57560637	MS.gene049531:CDS
CTTGTATCTATTACTCAGTG+AGG	0.604691	3.2:-57560336	None:intergenic
GCACATCTTGACTCAACATG+TGG	0.636747	3.2:+57560574	MS.gene049531:CDS
AGTTTCCAAAACAGCTACAG+AGG	0.652548	3.2:-57560621	None:intergenic
CATCTTGACTCAACATGTGG+AGG	0.658303	3.2:+57560577	MS.gene049531:CDS
TCACTGAGTAATAGATACAA+GGG	0.673241	3.2:+57560338	MS.gene049531:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATGTGGATTTGATAATGAT+TGG	+	chr3.2:57560474-57560493	MS.gene049531:CDS	25.0%
	GAAATTTCAATAGTTCAGTC+AGG	-	chr3.2:57560379-57560398	None:intergenic	30.0%
	TCACTGAGTAATAGATACAA+GGG	+	chr3.2:57560338-57560357	MS.gene049531:CDS	30.0%
!	CTCATATTTCAAGTACTTCT+TGG	-	chr3.2:57560258-57560277	None:intergenic	30.0%
!	GTTTCCTTTAAAAGAACAGA+AGG	-	chr3.2:57560317-57560336	None:intergenic	30.0%
!!	TGGATTTGATAATGATTGGT+TGG	+	chr3.2:57560478-57560497	MS.gene049531:CDS	30.0%
!!	TTTGATAATGATTGGTTGGA+TGG	+	chr3.2:57560482-57560501	MS.gene049531:CDS	30.0%
	AGCTGCATTGCAAAAATTGT+CGG	+	chr3.2:57560544-57560563	MS.gene049531:CDS	35.0%
	CTCACTGAGTAATAGATACA+AGG	+	chr3.2:57560337-57560356	MS.gene049531:CDS	35.0%
	CTTGTATCTATTACTCAGTG+AGG	-	chr3.2:57560339-57560358	None:intergenic	35.0%
	GGAAACTATCATTCAAACAG+AGG	+	chr3.2:57560637-57560656	MS.gene049531:CDS	35.0%
	TAACACATCCAACACACTAT+GGG	-	chr3.2:57560408-57560427	None:intergenic	35.0%
!	GCATCCTTCTGTTCTTTTAA+AGG	+	chr3.2:57560310-57560329	MS.gene049531:CDS	35.0%
	AGTTTCCAAAACAGCTACAG+AGG	-	chr3.2:57560624-57560643	None:intergenic	40.0%
	ATACAAGATGTGCGCAAATG+TGG	+	chr3.2:57560458-57560477	MS.gene049531:CDS	40.0%
	CATAGTGTGTTGGATGTGTT+AGG	+	chr3.2:57560407-57560426	MS.gene049531:CDS	40.0%
	CTAACACATCCAACACACTA+TGG	-	chr3.2:57560409-57560428	None:intergenic	40.0%
	CTTGAGAAACTTGTAAGCCT+GGG	-	chr3.2:57560526-57560545	None:intergenic	40.0%
	CATCCAACACACTATGGGTT+TGG	-	chr3.2:57560403-57560422	None:intergenic	45.0%
	CATCTTGACTCAACATGTGG+AGG	+	chr3.2:57560577-57560596	MS.gene049531:CDS	45.0%
	GCACATCTTGACTCAACATG+TGG	+	chr3.2:57560574-57560593	MS.gene049531:CDS	45.0%
	GCTTGAGAAACTTGTAAGCC+TGG	-	chr3.2:57560527-57560546	None:intergenic	45.0%
	TAGTTCAGTCAGGCAGTTGT+AGG	-	chr3.2:57560369-57560388	None:intergenic	45.0%
	TCTCCAAACCCATAGTGTGT+TGG	+	chr3.2:57560397-57560416	MS.gene049531:CDS	45.0%
!	GTCTTCCTCTGTAGCTGTTT+TGG	+	chr3.2:57560616-57560635	MS.gene049531:CDS	45.0%
!	GTTGAAAAGCGTGCTTTCCC+AGG	+	chr3.2:57560506-57560525	MS.gene049531:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	57560230	57560664	57560230	ID=MS.gene049531
chr3.2	mRNA	57560230	57560664	57560230	ID=MS.gene049531.t1;Parent=MS.gene049531
chr3.2	exon	57560230	57560664	57560230	ID=MS.gene049531.t1.exon1;Parent=MS.gene049531.t1
chr3.2	CDS	57560230	57560664	57560230	ID=cds.MS.gene049531.t1;Parent=MS.gene049531.t1

Gene Sequence

>MS.gene049531

ATGAGGGAGCTTAAGCAACTAGTCTCCAAGAAGTACTTGAAATATGAGAACTTGTCTTTGTTGACTGATTTCTTTGTTAAGCATCCTTCTGTTCTTTTAAAGGAAACCTCACTGAGTAATAGATACAAGGGTTATGCCTACAACTGCCTGACTGAACTATTGAAATTTCTCCAAACCCATAGTGTGTTGGATGTGTTAGGTTCAAGTCACTCTGAATTTGTTGAGTTAATACAAGATGTGCGCAAATGTGGATTTGATAATGATTGGTTGGATGGTGTTGAAAAGCGTGCTTTCCCAGGCTTACAAGTTTCTCAAGCTGCATTGCAAAAATTGTCGGACTCAAAGCACATCTTGACTCAACATGTGGAGGATCTTAAGCATCAATTGTCTTCCTCTGTAGCTGTTTTGGAAACTATCATTCAAACAGAGGCATAA

Protein sequence

>MS.gene049531.t1

MRELKQLVSKKYLKYENLSLLTDFFVKHPSVLLKETSLSNRYKGYAYNCLTELLKFLQTHSVLDVLGSSHSEFVELIQDVRKCGFDNDWLDGVEKRAFPGLQVSQAALQKLSDSKHILTQHVEDLKHQLSSSVAVLETIIQTEA