AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065274.t1 MTR_2g078630 90.909 99 9 0 21 119 157 255 3.51e-52 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATGATAGGAAGGAGAAAT+TGG 0.280630 2.2:+16109289 MS.gene065274:CDS
TCTTCGACTTAAACTTCCTT+CGG 0.309919 2.2:-16109389 None:intergenic
TGGAGGTTGTGGAGGATGAT+AGG 0.370455 2.2:+16109276 MS.gene065274:CDS
GGTTGATAAGAAAGATGAAT+TGG 0.382241 2.2:+16109256 None:intergenic
CTTCGACTTAAACTTCCTTC+GGG 0.398868 2.2:-16109388 None:intergenic
AGGGATGAGAATGCTGCTAC+AGG 0.407439 2.2:+16109591 MS.gene065274:CDS
TTGGAGGTTGTGGAGGGTGA+TGG 0.423876 2.2:+16109308 MS.gene065274:CDS
GAAAGATGAATTGGAGGTTG+TGG 0.426899 2.2:+16109265 None:intergenic
TCATGAGCCAATTCAGTCTC+TGG 0.427658 2.2:-16109552 None:intergenic
GATGGGAAAGGAGGAATTAG+AGG 0.454067 2.2:+16109326 MS.gene065274:CDS
TTCGGTTCCAGAGACTGAAT+TGG 0.458005 2.2:+16109545 MS.gene065274:CDS
GAAGGAGAAATTGGAGGTTG+TGG 0.462683 2.2:+16109298 MS.gene065274:CDS
TGGAGGTTGTGGAGGGTGAT+GGG 0.473652 2.2:+16109309 MS.gene065274:CDS
AGTGGAGCTTGTAGTTGTAC+TGG 0.482159 2.2:+16109503 MS.gene065274:CDS
GTGGAGGTTGATAATAAAGA+GGG 0.483649 2.2:+16109351 MS.gene065274:CDS
GGAGAAATTGGAGGTTGTGG+AGG 0.484384 2.2:+16109301 MS.gene065274:CDS
GGTTGTGGAGGATGATAGGA+AGG 0.491215 2.2:+16109280 MS.gene065274:CDS
AAAGGAGGAATTAGAGGTTG+TGG 0.506664 2.2:+16109332 MS.gene065274:CDS
CTTGAGGAAAATCAATGTCC+AGG 0.507160 2.2:+16109453 MS.gene065274:CDS
AAATCAATGTCCAGGGAGGT+TGG 0.510515 2.2:+16109461 MS.gene065274:CDS
GTGGAGGGTGATGGGAAAGG+AGG 0.531045 2.2:+16109317 MS.gene065274:CDS
CACAGGTGAGGAGAGTGCTT+CGG 0.532940 2.2:+16109527 MS.gene065274:CDS
TGATAGGAAGGAGAAATTGG+AGG 0.540799 2.2:+16109292 MS.gene065274:CDS
GAGAAATTGGAGGTTGTGGA+GGG 0.553893 2.2:+16109302 MS.gene065274:CDS
ATGAGAATCTCATAGATTCG+AGG 0.554232 2.2:+16109571 MS.gene065274:CDS
TGATAAGAAAGATGAATTGG+AGG 0.554759 2.2:+16109259 None:intergenic
GAGGAGAGTTGAAGCGACAA+AGG 0.555553 2.2:+16109419 MS.gene065274:CDS
GGAGGAATTAGAGGTTGTGG+AGG 0.562541 2.2:+16109335 MS.gene065274:CDS
TTACTCTCATCCAACCTCCC+TGG 0.565421 2.2:-16109471 None:intergenic
GTTGTGGAGGGTGATGGGAA+AGG 0.569706 2.2:+16109314 MS.gene065274:CDS
TGTGGAGGTTGATAATAAAG+AGG 0.575325 2.2:+16109350 MS.gene065274:CDS
TGAGAGTAAGAGTCAAGAAG+TGG 0.581346 2.2:+16109485 MS.gene065274:CDS
AGGAGAGTTGAAGCGACAAA+GGG 0.583432 2.2:+16109420 MS.gene065274:CDS
GGGATGAGAATGCTGCTACA+GGG 0.589542 2.2:+16109592 MS.gene065274:CDS
TGAGAATCTCATAGATTCGA+GGG 0.593640 2.2:+16109572 MS.gene065274:CDS
TGGAGGTTGATAATAAAGAG+GGG 0.599796 2.2:+16109352 MS.gene065274:CDS
AGATGAATTGGAGGTTGTGG+AGG 0.600338 2.2:+16109268 None:intergenic
AAGCGACAAAGGGTGATCAA+AGG 0.604108 2.2:+16109430 MS.gene065274:CDS
AAAGGGTGATCAAAGGCTTG+AGG 0.616565 2.2:+16109437 MS.gene065274:CDS
TTGAGGAAAATCAATGTCCA+GGG 0.618979 2.2:+16109454 MS.gene065274:CDS
GAATGCTGCTACAGGGAACG+AGG 0.643524 2.2:+16109599 MS.gene065274:CDS
CTTGTAGTTGTACTGGACAC+AGG 0.655379 2.2:+16109510 MS.gene065274:CDS
AGTTGTACTGGACACAGGTG+AGG 0.676260 2.2:+16109515 MS.gene065274:CDS
TTAAGTCGAAGAAAAGAACG+AGG 0.697476 2.2:+16109400 MS.gene065274:CDS
AGGAAAATCAATGTCCAGGG+AGG 0.716242 2.2:+16109457 MS.gene065274:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GGTTTAAATATTTTACCCGA+AGG + chr2.2:16109373-16109392 MS.gene065274:CDS 30.0%
ATGAGAATCTCATAGATTCG+AGG + chr2.2:16109571-16109590 MS.gene065274:CDS 35.0%
TCTTCGACTTAAACTTCCTT+CGG - chr2.2:16109392-16109411 None:intergenic 35.0%
TGAGAATCTCATAGATTCGA+GGG + chr2.2:16109572-16109591 MS.gene065274:CDS 35.0%
TTAAGTCGAAGAAAAGAACG+AGG + chr2.2:16109400-16109419 MS.gene065274:CDS 35.0%
TTGAGGAAAATCAATGTCCA+GGG + chr2.2:16109454-16109473 MS.gene065274:CDS 35.0%
!! GTGGAGGTTGATAATAAAGA+GGG + chr2.2:16109351-16109370 MS.gene065274:CDS 35.0%
!! TGGAGGTTGATAATAAAGAG+GGG + chr2.2:16109352-16109371 MS.gene065274:CDS 35.0%
!! TGTGGAGGTTGATAATAAAG+AGG + chr2.2:16109350-16109369 MS.gene065274:CDS 35.0%
AAAGGAGGAATTAGAGGTTG+TGG + chr2.2:16109332-16109351 MS.gene065274:CDS 40.0%
CTTCGACTTAAACTTCCTTC+GGG - chr2.2:16109391-16109410 None:intergenic 40.0%
CTTGAGGAAAATCAATGTCC+AGG + chr2.2:16109453-16109472 MS.gene065274:CDS 40.0%
GGATGATAGGAAGGAGAAAT+TGG + chr2.2:16109289-16109308 MS.gene065274:CDS 40.0%
TGAGAGTAAGAGTCAAGAAG+TGG + chr2.2:16109485-16109504 MS.gene065274:CDS 40.0%
TGATAGGAAGGAGAAATTGG+AGG + chr2.2:16109292-16109311 MS.gene065274:CDS 40.0%
AAAGGGTGATCAAAGGCTTG+AGG + chr2.2:16109437-16109456 MS.gene065274:CDS 45.0%
AAATCAATGTCCAGGGAGGT+TGG + chr2.2:16109461-16109480 MS.gene065274:CDS 45.0%
AAGCGACAAAGGGTGATCAA+AGG + chr2.2:16109430-16109449 MS.gene065274:CDS 45.0%
AGGAAAATCAATGTCCAGGG+AGG + chr2.2:16109457-16109476 MS.gene065274:CDS 45.0%
AGGAGAGTTGAAGCGACAAA+GGG + chr2.2:16109420-16109439 MS.gene065274:CDS 45.0%
AGTGGAGCTTGTAGTTGTAC+TGG + chr2.2:16109503-16109522 MS.gene065274:CDS 45.0%
CTTGTAGTTGTACTGGACAC+AGG + chr2.2:16109510-16109529 MS.gene065274:CDS 45.0%
GAAGGAGAAATTGGAGGTTG+TGG + chr2.2:16109298-16109317 MS.gene065274:CDS 45.0%
GAGAAATTGGAGGTTGTGGA+GGG + chr2.2:16109302-16109321 MS.gene065274:CDS 45.0%
GATGGGAAAGGAGGAATTAG+AGG + chr2.2:16109326-16109345 MS.gene065274:CDS 45.0%
TCATGAGCCAATTCAGTCTC+TGG - chr2.2:16109555-16109574 None:intergenic 45.0%
TTCGGTTCCAGAGACTGAAT+TGG + chr2.2:16109545-16109564 MS.gene065274:CDS 45.0%
AGGGATGAGAATGCTGCTAC+AGG + chr2.2:16109591-16109610 MS.gene065274:CDS 50.0%
AGTTGTACTGGACACAGGTG+AGG + chr2.2:16109515-16109534 MS.gene065274:CDS 50.0%
GAGGAGAGTTGAAGCGACAA+AGG + chr2.2:16109419-16109438 MS.gene065274:CDS 50.0%
GGAGAAATTGGAGGTTGTGG+AGG + chr2.2:16109301-16109320 MS.gene065274:CDS 50.0%
GGAGGAATTAGAGGTTGTGG+AGG + chr2.2:16109335-16109354 MS.gene065274:CDS 50.0%
GGGATGAGAATGCTGCTACA+GGG + chr2.2:16109592-16109611 MS.gene065274:CDS 50.0%
GGTTGTGGAGGATGATAGGA+AGG + chr2.2:16109280-16109299 MS.gene065274:CDS 50.0%
TGGAGGTTGTGGAGGATGAT+AGG + chr2.2:16109276-16109295 MS.gene065274:CDS 50.0%
TTACTCTCATCCAACCTCCC+TGG - chr2.2:16109474-16109493 None:intergenic 50.0%
GAATGCTGCTACAGGGAACG+AGG + chr2.2:16109599-16109618 MS.gene065274:CDS 55.0%
TGGAGGTTGTGGAGGGTGAT+GGG + chr2.2:16109309-16109328 MS.gene065274:CDS 55.0%
TTGGAGGTTGTGGAGGGTGA+TGG + chr2.2:16109308-16109327 MS.gene065274:CDS 55.0%
!! CACAGGTGAGGAGAGTGCTT+CGG + chr2.2:16109527-16109546 MS.gene065274:CDS 55.0%
!! GTTGTGGAGGGTGATGGGAA+AGG + chr2.2:16109314-16109333 MS.gene065274:CDS 55.0%
!! GTGGAGGGTGATGGGAAAGG+AGG + chr2.2:16109317-16109336 MS.gene065274:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 16109270 16109632 16109270 ID=MS.gene065274
chr2.2 mRNA 16109270 16109632 16109270 ID=MS.gene065274.t1;Parent=MS.gene065274
chr2.2 exon 16109270 16109632 16109270 ID=MS.gene065274.t1.exon1;Parent=MS.gene065274.t1
chr2.2 CDS 16109270 16109632 16109270 ID=cds.MS.gene065274.t1;Parent=MS.gene065274.t1
Gene Sequence

>MS.gene065274

ATGAATTGGAGGTTGTGGAGGATGATAGGAAGGAGAAATTGGAGGTTGTGGAGGGTGATGGGAAAGGAGGAATTAGAGGTTGTGGAGGTTGATAATAAAGAGGGGTTTAAATATTTTACCCGAAGGAAGTTTAAGTCGAAGAAAAGAACGAGGAGAGTTGAAGCGACAAAGGGTGATCAAAGGCTTGAGGAAAATCAATGTCCAGGGAGGTTGGATGAGAGTAAGAGTCAAGAAGTGGAGCTTGTAGTTGTACTGGACACAGGTGAGGAGAGTGCTTCGGTTCCAGAGACTGAATTGGCTCATGAGAATCTCATAGATTCGAGGGATGAGAATGCTGCTACAGGGAACGAGGAGAAAAATTGA
Protein sequence

>MS.gene065274.t1

MNWRLWRMIGRRNWRLWRVMGKEELEVVEVDNKEGFKYFTRRKFKSKKRTRRVEATKGDQRLEENQCPGRLDESKSQEVELVVVLDTGEESASVPETELAHENLIDSRDENAATGNEEKN