AlfalfaGEDB | MS.gene06529

M. sativa cultivar XinJiangDaYe / MS.gene06529

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06529.t1	XP_013461513.1	96.2	312	11	1	1	312	1	311	1.10E-158	569.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06529.t1	O22873	48.8	330	111	10	5	290	32	347	1.9e-58	227.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06529.t1	A0A072V2G2	96.2	312	11	1	1	312	1	311	7.9e-159	569.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene06529.t1	TF	bZIP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049400	MS.gene06529	0.979777	1.02E-148	-1.69E-46
MS.gene050133	MS.gene06529	0.805286	1.44E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06529.t1	MTR_3g094020	98.397	312	4	1	1	312	1	311	0.0	629
MS.gene06529.t1	MTR_5g075390	52.366	317	131	8	7	312	51	358	4.53e-94	283
MS.gene06529.t1	MTR_3g467120	46.705	349	134	11	7	312	38	377	2.82e-87	266
MS.gene06529.t1	MTR_2g099050	43.056	288	89	10	7	231	72	347	1.15e-50	173
MS.gene06529.t1	MTR_6g034945	48.624	218	87	6	7	201	28	243	8.20e-48	162
MS.gene06529.t1	MTR_4g072090	69.492	118	36	0	90	207	341	458	2.21e-46	164
MS.gene06529.t1	MTR_4g072090	66.981	106	35	0	90	195	341	446	2.54e-38	141
MS.gene06529.t1	MTR_1g023690	51.923	156	73	1	92	247	370	523	1.55e-29	118
MS.gene06529.t1	MTR_2g090900	46.809	94	50	0	108	201	190	283	6.09e-18	83.2
MS.gene06529.t1	MTR_2g090900	46.809	94	50	0	108	201	190	283	1.09e-17	82.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06529.t1	AT2G40620	51.057	331	98	11	5	288	32	345	8.79e-84	257
MS.gene06529.t1	AT1G06850	51.082	231	86	4	78	301	116	326	1.47e-68	217
MS.gene06529.t1	AT2G31370	46.275	255	86	7	7	215	58	307	1.04e-53	180
MS.gene06529.t1	AT2G31370	46.275	255	86	7	7	215	58	307	1.26e-53	180
MS.gene06529.t1	AT2G31370	46.275	255	86	7	7	215	58	307	1.26e-53	180
MS.gene06529.t1	AT2G31370	46.275	255	86	7	7	215	58	307	1.26e-53	180
MS.gene06529.t1	AT2G31370	46.275	255	86	7	7	215	58	307	1.26e-53	180
MS.gene06529.t1	AT2G31370	46.275	255	86	7	7	215	58	307	1.26e-53	180
MS.gene06529.t1	AT2G31370	46.275	255	86	7	7	215	58	307	1.26e-53	180
MS.gene06529.t1	AT1G06070	45.200	250	94	6	7	217	73	318	1.24e-51	176

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Find 97 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATACCGAGTTGAAGCTT+CGG	0.538764	3.2:+78820336	MS.gene06529:CDS
AAACTGAGGCTGGTGGGGAC+TGG	0.421487	3.2:-78821583	None:intergenic
AAAGACCTCTTGGCGCGTTT+AGG	0.396524	3.2:-78819401	None:intergenic
AACATGGTCCAAACGATCTT+AGG	0.241223	3.2:+78821517	MS.gene06529:CDS
ACAGAAACGGAGGGACCATC+AGG	0.491647	3.2:-78821708	None:intergenic
ACATGGTCCAAACGATCTTA+GGG	0.515048	3.2:+78821518	MS.gene06529:CDS
ACCCACTTCATATGATTTGT+CGG	0.433055	3.2:+78821611	MS.gene06529:CDS
ACGGCGGTGACCGGCATTAG+CGG	0.537421	3.2:-78819279	None:intergenic
ACTGGATCCATTAGATGAGA+GGG	0.595904	3.2:-78821565	None:intergenic
AGAGAGGAAAGCTTGTTATG+TGG	0.577168	3.2:+78819536	MS.gene06529:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTCCTTATATCAGTGGT+GGG	+	chr3.2:78820234-78820253	MS.gene06529:intron	30.0%
AGTCTCTTAAGTTTGATAGA+TGG	+	chr3.2:78820566-78820585	MS.gene06529:intron	30.0%
ATGAAAATGTTGCATTTGCA+TGG	-	chr3.2:78821544-78821563	None:intergenic	30.0%
ATGTACAACGATTTGAAAAC+TGG	-	chr3.2:78821237-78821256	None:intergenic	30.0%
ATGTTGTCAAGTGATTCTAT+CGG	+	chr3.2:78821636-78821655	MS.gene06529:CDS	30.0%
CAATTACAAACTAAATGAGC+TGG	+	chr3.2:78820839-78820858	MS.gene06529:intron	30.0%
CACTGAATTAATGTAAGCAT+CGG	+	chr3.2:78820514-78820533	MS.gene06529:intron	30.0%
CATATGAAGATTCTGTTCAA+GGG	+	chr3.2:78819978-78819997	MS.gene06529:intron	30.0%
GAAGTAGTGATAAATGAACA+TGG	-	chr3.2:78821157-78821176	None:intergenic	30.0%
GGCCTTCAAAAAAAAAAAGA+GGG	+	chr3.2:78820054-78820073	MS.gene06529:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	78819074	78821755	78819074	ID=MS.gene06529
chr3.2	mRNA	78819074	78821755	78819074	ID=MS.gene06529.t1;Parent=MS.gene06529
chr3.2	exon	78819074	78819417	78819074	ID=MS.gene06529.t1.exon1;Parent=MS.gene06529.t1
chr3.2	CDS	78819074	78819417	78819074	ID=cds.MS.gene06529.t1;Parent=MS.gene06529.t1
chr3.2	exon	78819500	78819632	78819500	ID=MS.gene06529.t1.exon2;Parent=MS.gene06529.t1
chr3.2	CDS	78819500	78819632	78819500	ID=cds.MS.gene06529.t1;Parent=MS.gene06529.t1
chr3.2	exon	78820311	78820395	78820311	ID=MS.gene06529.t1.exon3;Parent=MS.gene06529.t1
chr3.2	CDS	78820311	78820395	78820311	ID=cds.MS.gene06529.t1;Parent=MS.gene06529.t1
chr3.2	exon	78821379	78821755	78821379	ID=MS.gene06529.t1.exon4;Parent=MS.gene06529.t1
chr3.2	CDS	78821379	78821755	78821379	ID=cds.MS.gene06529.t1;Parent=MS.gene06529.t1

Gene Sequence

>MS.gene06529

ATGTCGTCGTTCCCGTTCCACCGTCGATCTCAATCGGAGGTACATTTCCGAATCCCCGACGATTTCGACCTCGAAATGGATCCCTTCGACTTCGACGCTTCACCGCTCCAGTTCCAAGATCCACCACCACCGCAACAACACGACGATCTCCTTTCCTCTTACATCGATTCCGATAATTCCGGATCCAAACTCAATTCTCCTACCGCCGCTAATGCCGGTCACCGCCGTAGCAATTCCGCCGATACATCTTCTTCCTTGTTGTCTGAAGGAATCGAATCTAAGAAAGCTATGTCTCCTGATAAACTTGCTCAATTGTGGACCGTTGATCCTAAACGCGCCAAGAGGTCTTTCTTTTATTGCAATTTTCATTTTGTTTTTTTGTTTATTGAATTGAACTTGTTTTTATAGATCGTGGATTTGATTCAGGATTCTGGCGAATCGTCAATCTGCGGCGAGGTCGAAAGAGAGGAAAGCTTGTTATGTGGTTGAGCTTGAAAGGAAAATTCATACTCTTCAAACTGAAGCAACTACTCTTTCTGCTCAATTAAATCTTTTCCAGGTTATTACTCATCATTGCCTAAGTTGAATCAAAAATATTATGATGTATAGTTTAAATATACAAATATGAGAGATAATAATGAATTTCAACATGATTATATATTTGTTGTGAAGCTTAAATGGGCTGTGTAGTTAATGTCAAGAATAGTAAGTGATAAATCCGATCAGAGAGGTAGGTTGAAATGGAGAGAAGCGTCTGTAGCGATGAGATTAGGGGTGGTATTTGCAAGCACTCTGACGCTTAAGTTAGGGAGAGAGCAAATAAGGGAATCTGAAGAGATAATAATGAATTTCAACATGATTATATATTTGTTGTGGCCATTAATGAGGGTTGGGACGTTATGGTCATATGAAGATTCTGTTCAAGGGAATCTGTGTGTGGGAACGTCGAATTGGACTCCACCATATTCACAGGTGGAGTGGGCCTTCAAAAAAAAAAAGAGGGAGTCTCTAAGTCCAATCCAGAACAGTTACCCTATCCTTTGGTTATTTTTTTTTTTTTTCCATTGGGCTATTGGTTTGCTTTTCACTCTTGTGGCAGTTCATTTTTCAAAATGGACATGCCTTTGTTTCCTTTAATTGAACTTGGTTTCCAGTTTGTTAAATTCCTTATATCAGTGGTGGGCTTTCATTTTATGTTCTGTGAAACTTATTCATTCAAAAACAACATCTTTTTCAGAGAGATACAACTGGTCTGTCAAGTGAAAATACCGAGTTGAAGCTTCGGTTACAAACCATGGAGCAACAAGCTAAGCTATGTGATGGTACGTCTTAGCCATGCAAATATGCTACACTTTATTTCCTTGTTCTTTCTTGTTTTGCTGGTTTTTGTTGTTGTTGTTTTCCATCAGTATAATATGAAAGGCCATTAACTTAGTTTAACACTGAATTAATGTAAGCATCGGTAATTTAACTTTGACTTAGTTGTAATTTTAGTCTCTTAAGTTTGATAGATGGGTGATTTTATTTCCTATAGTTTCTGTTAGAATATGCGAAGATATCTTTCATTTGTTTATATTATGTTAGAGGATATAAGTTATCTTGTAGTATCGGTTTCTAATCCTCATACTTTTCAATTCAATGAACTCCGTCCAAACATTAATGTTTTGTCTATTTATTAATATTTGAGCCTACGTGGATTATGGTTTGTGACATGTCAACTGTCTCAGATGACATGGTAGTCCATTTAAAGGATAAATGCTGTATCCAATTACAAACTAAATGAGCTGGCATTGACGTCTGACGACAACAGCCTCGTATGACATGTAATTTTTTTGTGCCATGCCATGTCAGCTCAAGTCGTTGCATCATTACAAGATAGCATTCATTATAGCTTACATTCTTAGTAATTGGATGGTATATTAACACTTGGTCTAGACTTCATTCATTTGGAAAGTGTGGGTGACTTGGCTTAAACAATCGAAACTGTAGAAAAGATACTACAGTGATTGAAGCCTTTCGGTTGACATCTTATTAATTTAGCTCAATTTATATCACTTAATATATTATTTAATGTTGAAAACCATGTTCATTTATCACTACTTCAACTAAGGTTAAACCAATGAACCATGAATCAAAACCTTCACTACTTCAATGACTAGTCCAGTTTTCAAATCGTTGTACATGATCAATAACCTAAAAGTAGTGTTTCTGTTTTGTTAAAATTTCAGCTTATATGTGACATACAATTCATTTACATTACTCAAACTTCCAGTTTTAAAAATAATAATTATTATAAAATTTACAGCTCTAAATGAAGCATTGAAGAACGAAGTTGATAGGCTCAAGCTTGCTACTGGAGAGGTACCAACCCATGCTGATACCTATGGTTTGGGAATGCATCAATTTTCATATTCTCAAGCTTCATCCTTCATGCACCAGCCACAACATGGTCCAAACGATCTTAGGGCCATGCAAATGCAACATTTTCATTCCCTCTCATCTAATGGATCCAGTCCCCACCAGCCTCAGTTTGATCTACCCACTTCATATGATTTGTCGGAAATGTTGTCAAGTGATTCTATCGGCCAATTTCAGGGGCTGGACATTGGCAACAGGGTTTCTCATGTTCTGATGCCTGATGGTCCCTCCGTTTCTGTTAATAAAACCAACAACGCCTTTTGA

Protein sequence

>MS.gene06529.t1

MSSFPFHRRSQSEVHFRIPDDFDLEMDPFDFDASPLQFQDPPPPQQHDDLLSSYIDSDNSGSKLNSPTAANAGHRRSNSADTSSSLLSEGIESKKAMSPDKLAQLWTVDPKRAKRILANRQSAARSKERKACYVVELERKIHTLQTEATTLSAQLNLFQRDTTGLSSENTELKLRLQTMEQQAKLCDALNEALKNEVDRLKLATGEVPTHADTYGLGMHQFSYSQASSFMHQPQHGPNDLRAMQMQHFHSLSSNGSSPHQPQFDLPTSYDLSEMLSSDSIGQFQGLDIGNRVSHVLMPDGPSVSVNKTNNAF