Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065591.t1 | AES94950.1 | 83.2 | 107 | 7 | 2 | 1 | 107 | 1 | 96 | 3.30E-38 | 167.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065591.t1 | G7KDJ9 | 83.2 | 107 | 7 | 2 | 1 | 107 | 1 | 96 | 2.4e-38 | 167.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054892 | MS.gene065591 | 0.812371 | 4.38E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene065591 | 0.813961 | 1.96E-51 | -1.69E-46 |
| MS.gene057155 | MS.gene065591 | 0.816789 | 4.61E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065591.t1 | MTR_5g020110 | 85.047 | 107 | 5 | 2 | 1 | 107 | 1 | 96 | 1.42e-57 | 172 |
| MS.gene065591.t1 | MTR_5g020090 | 67.273 | 110 | 25 | 2 | 1 | 107 | 1 | 102 | 1.40e-46 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAACACAAGAAGAATAAA+AGG | 0.259149 | 5.3:-11511062 | MS.gene065591:CDS |
| CGCCACCTCCTCCGCCTCGT+TGG | 0.367228 | 5.3:+11510990 | None:intergenic |
| ATATTCTTCATTTGTCTCCA+TGG | 0.393537 | 5.3:+11511083 | None:intergenic |
| CTCCTCCGCCTCGTTGGGAC+TGG | 0.400046 | 5.3:+11510996 | None:intergenic |
| AAGAAGAATAAAAGGGGTAT+TGG | 0.401583 | 5.3:-11511054 | MS.gene065591:CDS |
| GGAGGAGGTGGCGGTGAAGA+AGG | 0.405166 | 5.3:-11510980 | MS.gene065591:CDS |
| GCCACCTCCTCCGCCTCGTT+GGG | 0.405210 | 5.3:+11510991 | None:intergenic |
| TCCCAACGAGGCGGAGGAGG+TGG | 0.427668 | 5.3:-11510992 | MS.gene065591:CDS |
| ATCAGTCACCACCCTTGAAC+TGG | 0.429303 | 5.3:+11510913 | None:intergenic |
| TCGGAATGTGTTAAAGTTGG+TGG | 0.447025 | 5.3:-11511150 | MS.gene065591:CDS |
| CAGTCCCAACGAGGCGGAGG+AGG | 0.461103 | 5.3:-11510995 | MS.gene065591:CDS |
| TGAACACAAGAAGAATAAAA+GGG | 0.482825 | 5.3:-11511061 | MS.gene065591:CDS |
| ATCATCAGAATCTGTAACTT+CGG | 0.488685 | 5.3:-11511169 | MS.gene065591:CDS |
| CAACGAGGCGGAGGAGGTGG+CGG | 0.501673 | 5.3:-11510989 | MS.gene065591:CDS |
| TTCCAGTCCCAACGAGGCGG+AGG | 0.517997 | 5.3:-11510998 | MS.gene065591:CDS |
| AGGAGAAGAAACGTTCGTCT+TGG | 0.533201 | 5.3:-11510960 | MS.gene065591:CDS |
| TATGAAAGACTCTCACTTGG+TGG | 0.550639 | 5.3:-11511126 | MS.gene065591:CDS |
| ACTTCGGAATGTGTTAAAGT+TGG | 0.563777 | 5.3:-11511153 | MS.gene065591:CDS |
| GTTAGGTTGTCAAGATCAGA+AGG | 0.595454 | 5.3:-11511201 | MS.gene065591:CDS |
| TGAAAGACTCTCACAAACCA+TGG | 0.604467 | 5.3:-11511100 | MS.gene065591:CDS |
| AACGCTGGCCAGTTCAAGGG+TGG | 0.630598 | 5.3:-11510921 | MS.gene065591:CDS |
| GAACACAAGAAGAATAAAAG+GGG | 0.652443 | 5.3:-11511060 | MS.gene065591:CDS |
| GAGAATTTCCAGTCCCAACG+AGG | 0.655349 | 5.3:-11511004 | MS.gene065591:CDS |
| GGTTATGAAAGACTCTCACT+TGG | 0.659276 | 5.3:-11511129 | MS.gene065591:CDS |
| AATTTCCAGTCCCAACGAGG+CGG | 0.689181 | 5.3:-11511001 | MS.gene065591:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAACACAAGAAGAATAAA+AGG | - | chr5.3:11511075-11511094 | MS.gene065591:CDS | 25.0% |
| ! | TGAACACAAGAAGAATAAAA+GGG | - | chr5.3:11511076-11511095 | MS.gene065591:CDS | 25.0% |
| !!! | TGGAATTTAGAAAAAGTGTT+AGG | - | chr5.3:11510919-11510938 | MS.gene065591:CDS | 25.0% |
| ATATTCTTCATTTGTCTCCA+TGG | + | chr5.3:11511057-11511076 | None:intergenic | 30.0% | |
| ATCATCAGAATCTGTAACTT+CGG | - | chr5.3:11510968-11510987 | MS.gene065591:CDS | 30.0% | |
| GAACACAAGAAGAATAAAAG+GGG | - | chr5.3:11511077-11511096 | MS.gene065591:CDS | 30.0% | |
| ! | AAGAAGAATAAAAGGGGTAT+TGG | - | chr5.3:11511083-11511102 | MS.gene065591:CDS | 30.0% |
| ACTTCGGAATGTGTTAAAGT+TGG | - | chr5.3:11510984-11511003 | MS.gene065591:CDS | 35.0% | |
| GGTTATGAAAGACTCTCACT+TGG | - | chr5.3:11511008-11511027 | MS.gene065591:CDS | 40.0% | |
| TATGAAAGACTCTCACTTGG+TGG | - | chr5.3:11511011-11511030 | MS.gene065591:CDS | 40.0% | |
| TCGGAATGTGTTAAAGTTGG+TGG | - | chr5.3:11510987-11511006 | MS.gene065591:CDS | 40.0% | |
| TGAAAGACTCTCACAAACCA+TGG | - | chr5.3:11511037-11511056 | MS.gene065591:CDS | 40.0% | |
| ! | GTTAGGTTGTCAAGATCAGA+AGG | - | chr5.3:11510936-11510955 | MS.gene065591:CDS | 40.0% |
| AAAAACGCTGGCCAGTTCAA+GGG | - | chr5.3:11511213-11511232 | MS.gene065591:CDS | 45.0% | |
| AGGAGAAGAAACGTTCGTCT+TGG | - | chr5.3:11511177-11511196 | MS.gene065591:CDS | 45.0% | |
| TTCCAGATCCACAAAAACGC+TGG | - | chr5.3:11511201-11511220 | MS.gene065591:CDS | 45.0% | |
| AATTTCCAGTCCCAACGAGG+CGG | - | chr5.3:11511136-11511155 | MS.gene065591:CDS | 50.0% | |
| CAAAAACGCTGGCCAGTTCA+AGG | - | chr5.3:11511212-11511231 | MS.gene065591:CDS | 50.0% | |
| GAGAATTTCCAGTCCCAACG+AGG | - | chr5.3:11511133-11511152 | MS.gene065591:CDS | 50.0% | |
| ! | TGAACTGGCCAGCGTTTTTG+TGG | + | chr5.3:11511212-11511231 | None:intergenic | 50.0% |
| ! | GGCCAGCGTTTTTGTGGATC+TGG | + | chr5.3:11511206-11511225 | None:intergenic | 55.0% |
| AACGCTGGCCAGTTCAAGGG+TGG | - | chr5.3:11511216-11511235 | MS.gene065591:CDS | 60.0% | |
| TTCCAGTCCCAACGAGGCGG+AGG | - | chr5.3:11511139-11511158 | MS.gene065591:CDS | 65.0% | |
| ! | GGAGGAGGTGGCGGTGAAGA+AGG | - | chr5.3:11511157-11511176 | MS.gene065591:CDS | 65.0% |
| CAACGAGGCGGAGGAGGTGG+CGG | - | chr5.3:11511148-11511167 | MS.gene065591:CDS | 70.0% | |
| CAGTCCCAACGAGGCGGAGG+AGG | - | chr5.3:11511142-11511161 | MS.gene065591:CDS | 70.0% | |
| CTCCTCCGCCTCGTTGGGAC+TGG | + | chr5.3:11511144-11511163 | None:intergenic | 70.0% | |
| GCCACCTCCTCCGCCTCGTT+GGG | + | chr5.3:11511149-11511168 | None:intergenic | 70.0% | |
| TCCCAACGAGGCGGAGGAGG+TGG | - | chr5.3:11511145-11511164 | MS.gene065591:CDS | 70.0% | |
| CGCCACCTCCTCCGCCTCGT+TGG | + | chr5.3:11511150-11511169 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 11510918 | 11511241 | 11510918 | ID=MS.gene065591 |
| chr5.3 | mRNA | 11510918 | 11511241 | 11510918 | ID=MS.gene065591.t1;Parent=MS.gene065591 |
| chr5.3 | exon | 11510918 | 11511241 | 11510918 | ID=MS.gene065591.t1.exon1;Parent=MS.gene065591.t1 |
| chr5.3 | CDS | 11510918 | 11511241 | 11510918 | ID=cds.MS.gene065591.t1;Parent=MS.gene065591.t1 |
| Gene Sequence |
| Protein sequence |
