Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065951.t1 | XP_013443646.1 | 95.9 | 170 | 5 | 2 | 1 | 169 | 62 | 230 | 1.30E-76 | 295.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065951.t1 | Q38847 | 46.7 | 169 | 73 | 2 | 1 | 169 | 62 | 213 | 9.3e-28 | 124.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065951.t1 | A0A072TKE2 | 95.9 | 170 | 5 | 2 | 1 | 169 | 62 | 230 | 9.1e-77 | 295.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055311 | MS.gene065951 | 0.801856 | 7.39E-49 | -1.69E-46 |
| MS.gene059171 | MS.gene065951 | 0.803993 | 2.67E-49 | -1.69E-46 |
| MS.gene060463 | MS.gene065951 | 0.800105 | 1.68E-48 | -1.69E-46 |
| MS.gene060465 | MS.gene065951 | 0.809276 | 2.05E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGACACCACATTGCAATT+AGG | 0.257094 | 4.1:+58540916 | MS.gene065951:CDS |
| CAGACACCACATTGCAATTA+GGG | 0.281450 | 4.1:+58540917 | MS.gene065951:CDS |
| AAGAACTCCTTTGTAGATAA+TGG | 0.328915 | 4.1:+58540844 | MS.gene065951:CDS |
| TACTTCCGAAGGAAGATTCT+AGG | 0.353361 | 4.1:+58539382 | MS.gene065951:CDS |
| CTTATCTGGATTGGGAATAA+AGG | 0.357344 | 4.1:+58539583 | MS.gene065951:CDS |
| GGAATTACTCATGGAACAAC+TGG | 0.394806 | 4.1:+58539943 | MS.gene065951:CDS |
| CTGTTGGGAAAGGACTTATC+TGG | 0.395296 | 4.1:+58539569 | MS.gene065951:CDS |
| GGGAAAGGACTTATCTGGAT+TGG | 0.415856 | 4.1:+58539574 | MS.gene065951:CDS |
| GGCATAACATGTTCTGTCAT+TGG | 0.418254 | 4.1:-58540790 | None:intergenic |
| GGAAAGGACTTATCTGGATT+GGG | 0.419728 | 4.1:+58539575 | MS.gene065951:CDS |
| GAATGAAACTTTGCGAAGAC+AGG | 0.464677 | 4.1:+58540641 | MS.gene065951:CDS |
| CCAGGAGCAGAAGGCTATGC+TGG | 0.465487 | 4.1:+58540617 | MS.gene065951:intron |
| ACTGGAGCAGTCTCGAATAC+AGG | 0.492606 | 4.1:+58539961 | MS.gene065951:CDS |
| TGAGCAGTTTCTAGTCAAGC+TGG | 0.492674 | 4.1:-58540878 | None:intergenic |
| TGTGACTATAGACGACTGTT+GGG | 0.503182 | 4.1:+58539554 | MS.gene065951:intron |
| AAGGACCTTCCTCTCCTTCA+CGG | 0.505318 | 4.1:-58539648 | None:intergenic |
| TTGTGACTATAGACGACTGT+TGG | 0.511744 | 4.1:+58539553 | MS.gene065951:intron |
| ACCATCCTAGAATCTTCCTT+CGG | 0.523873 | 4.1:-58539387 | None:intergenic |
| TTCTTCAGAGAGTGCATCAG+TGG | 0.529288 | 4.1:+58539236 | MS.gene065951:CDS |
| TTATTCTTTCCAGGAGCAGA+AGG | 0.530019 | 4.1:+58540608 | MS.gene065951:intron |
| CTTACAGGAGGAATTACTCA+TGG | 0.556408 | 4.1:+58539934 | MS.gene065951:intron |
| TGGCGAAACTGAAGATAAGA+TGG | 0.563353 | 4.1:-58540811 | None:intergenic |
| TTATCTTCAGTTTCGCCATG+TGG | 0.585861 | 4.1:+58540816 | MS.gene065951:CDS |
| GAGCAGTTTCTAGTCAAGCT+GGG | 0.589240 | 4.1:-58540877 | None:intergenic |
| CTATAGACGACTGTTGGGAA+AGG | 0.593110 | 4.1:+58539559 | MS.gene065951:intron |
| AGGAGTTCTTTCTTTCCACA+TGG | 0.598924 | 4.1:-58540831 | None:intergenic |
| CTTAACACCATTATCTACAA+AGG | 0.604124 | 4.1:-58540851 | None:intergenic |
| ATCATCCGTGAAGGAGAGGA+AGG | 0.607253 | 4.1:+58539643 | MS.gene065951:CDS |
| GATTATCATCCGTGAAGGAG+AGG | 0.609896 | 4.1:+58539639 | MS.gene065951:CDS |
| TTCAGAGAGTGCATCAGTGG+AGG | 0.615743 | 4.1:+58539239 | MS.gene065951:CDS |
| CGAGGGATTATCATCCGTGA+AGG | 0.619271 | 4.1:+58539634 | MS.gene065951:CDS |
| TCCGAAGGAAGATTCTAGGA+TGG | 0.625002 | 4.1:+58539386 | MS.gene065951:CDS |
| CTAGAGCAGCAAATTAACGA+GGG | 0.653295 | 4.1:+58539617 | MS.gene065951:CDS |
| TGTTCATTAGATACTTCCGA+AGG | 0.661567 | 4.1:+58539371 | MS.gene065951:intron |
| TCTAGAGCAGCAAATTAACG+AGG | 0.688872 | 4.1:+58539616 | MS.gene065951:CDS |
| AGTGGAGGAATATAAAACAG+AGG | 0.706403 | 4.1:+58539254 | MS.gene065951:CDS |
| ATAATACCCTAATTGCAATG+TGG | 0.720756 | 4.1:-58540923 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATATCAAGAAAAATACA+TGG | - | chr4.1:58540208-58540227 | None:intergenic | 20.0% |
| !! | TAAAACAGAAAAAACAAAAG+AGG | - | chr4.1:58540353-58540372 | None:intergenic | 20.0% |
| !!! | TTAATACGATTTTAATCTTC+TGG | + | chr4.1:58540676-58540695 | MS.gene065951:intron | 20.0% |
| ! | AAAGACGAAAAAATAGGAAA+GGG | - | chr4.1:58539681-58539700 | None:intergenic | 25.0% |
| ! | AAATCAAAAAGGAGAAGAAA+AGG | - | chr4.1:58540384-58540403 | None:intergenic | 25.0% |
| ! | AAATCAGTAGTTAACATTTG+AGG | + | chr4.1:58539991-58540010 | MS.gene065951:intron | 25.0% |
| ! | AATCAAAAAGGAGAAGAAAA+GGG | - | chr4.1:58540383-58540402 | None:intergenic | 25.0% |
| ! | GGATAAGAAAGAAATCAAAA+AGG | - | chr4.1:58540395-58540414 | None:intergenic | 25.0% |
| ! | TTGCAATGACATATAAATTC+TGG | + | chr4.1:58540169-58540188 | MS.gene065951:intron | 25.0% |
| !! | TTGTTTGATTTATTCTTTCC+AGG | + | chr4.1:58540599-58540618 | MS.gene065951:intron | 25.0% |
| AAGAACTCCTTTGTAGATAA+TGG | + | chr4.1:58540844-58540863 | MS.gene065951:CDS | 30.0% | |
| AATAGGAAAGGGTTAAGATA+AGG | - | chr4.1:58539670-58539689 | None:intergenic | 30.0% | |
| AGAAATATTGCCTACCAAAT+AGG | - | chr4.1:58540126-58540145 | None:intergenic | 30.0% | |
| AGTTAGAACAATTGTTATGC+AGG | - | chr4.1:58539349-58539368 | None:intergenic | 30.0% | |
| ATCAAGAAAAATACATGGAC+TGG | - | chr4.1:58540203-58540222 | None:intergenic | 30.0% | |
| CTTAACACCATTATCTACAA+AGG | - | chr4.1:58540854-58540873 | None:intergenic | 30.0% | |
| GAAAGACGAAAAAATAGGAA+AGG | - | chr4.1:58539682-58539701 | None:intergenic | 30.0% | |
| ! | AAACCACAACTTAATTTTGG+TGG | - | chr4.1:58539870-58539889 | None:intergenic | 30.0% |
| ! | AAATCTGATAATGCTAAAGC+AGG | - | chr4.1:58540270-58540289 | None:intergenic | 30.0% |
| ! | ACTTTGAGAATAAGCCTATT+TGG | + | chr4.1:58540109-58540128 | MS.gene065951:intron | 30.0% |
| ! | CAGAAACCACAACTTAATTT+TGG | - | chr4.1:58539873-58539892 | None:intergenic | 30.0% |
| !! | AACCACAACTTAATTTTGGT+GGG | - | chr4.1:58539869-58539888 | None:intergenic | 30.0% |
| !!! | TTAACATTTGAGGTTTTGAG+TGG | + | chr4.1:58540001-58540020 | MS.gene065951:intron | 30.0% |
| AACAGGCAATTTGTAAAGGA+TGG | + | chr4.1:58539779-58539798 | MS.gene065951:intron | 35.0% | |
| AGTGGAGGAATATAAAACAG+AGG | + | chr4.1:58539254-58539273 | MS.gene065951:CDS | 35.0% | |
| ATTTGCATTACTGCGCAATA+AGG | + | chr4.1:58539505-58539524 | MS.gene065951:intron | 35.0% | |
| CGCAGTAATGCAAATACAAA+TGG | - | chr4.1:58539500-58539519 | None:intergenic | 35.0% | |
| GAAACAAACTGACAAAACAG+AGG | - | chr4.1:58540456-58540475 | None:intergenic | 35.0% | |
| GAACAGATGTCAAGGATTAA+AGG | - | chr4.1:58539915-58539934 | None:intergenic | 35.0% | |
| GGGAAGAAAGACGAAAAAAT+AGG | - | chr4.1:58539687-58539706 | None:intergenic | 35.0% | |
| TCGAAAAAGCAAAGAACAGA+AGG | - | chr4.1:58540416-58540435 | None:intergenic | 35.0% | |
| TGAGAATAAGCCTATTTGGT+AGG | + | chr4.1:58540113-58540132 | MS.gene065951:intron | 35.0% | |
| TGTTCATTAGATACTTCCGA+AGG | + | chr4.1:58539371-58539390 | MS.gene065951:intron | 35.0% | |
| ! | CTTATCTGGATTGGGAATAA+AGG | + | chr4.1:58539583-58539602 | MS.gene065951:CDS | 35.0% |
| !! | ACCACAACTTAATTTTGGTG+GGG | - | chr4.1:58539868-58539887 | None:intergenic | 35.0% |
| !! | GTGGTTATTGGCTTAGTTAT+AGG | + | chr4.1:58540020-58540039 | MS.gene065951:intron | 35.0% |
| !!! | TTGAGGTTTTGAGTGGTTAT+TGG | + | chr4.1:58540008-58540027 | MS.gene065951:intron | 35.0% |
| ACCATCCTAGAATCTTCCTT+CGG | - | chr4.1:58539390-58539409 | None:intergenic | 40.0% | |
| AGGAGTTCTTTCTTTCCACA+TGG | - | chr4.1:58540834-58540853 | None:intergenic | 40.0% | |
| CCTTTACAAATTGCCTGTTG+CGG | - | chr4.1:58539778-58539797 | None:intergenic | 40.0% | |
| CTAGAGCAGCAAATTAACGA+GGG | + | chr4.1:58539617-58539636 | MS.gene065951:CDS | 40.0% | |
| CTGACAAAACAGAGGAAAAG+AGG | - | chr4.1:58540448-58540467 | None:intergenic | 40.0% | |
| CTTACAGGAGGAATTACTCA+TGG | + | chr4.1:58539934-58539953 | MS.gene065951:intron | 40.0% | |
| CTTCAATCTGCAGTAGAGAT+TGG | - | chr4.1:58540753-58540772 | None:intergenic | 40.0% | |
| GAACAATTGTTATGCAGGCA+AGG | - | chr4.1:58539344-58539363 | None:intergenic | 40.0% | |
| GAATGAAACTTTGCGAAGAC+AGG | + | chr4.1:58540641-58540660 | MS.gene065951:CDS | 40.0% | |
| GATCGTATACGAGAAGAGAA+AGG | - | chr4.1:58540482-58540501 | None:intergenic | 40.0% | |
| GGAAAGGACTTATCTGGATT+GGG | + | chr4.1:58539575-58539594 | MS.gene065951:CDS | 40.0% | |
| GGAATTACTCATGGAACAAC+TGG | + | chr4.1:58539943-58539962 | MS.gene065951:CDS | 40.0% | |
| GGCATAACATGTTCTGTCAT+TGG | - | chr4.1:58540793-58540812 | None:intergenic | 40.0% | |
| TACTTCCGAAGGAAGATTCT+AGG | + | chr4.1:58539382-58539401 | MS.gene065951:CDS | 40.0% | |
| TCAGACACCACATTGCAATT+AGG | + | chr4.1:58540916-58540935 | MS.gene065951:CDS | 40.0% | |
| TCCTGTAAGAACAGATGTCA+AGG | - | chr4.1:58539923-58539942 | None:intergenic | 40.0% | |
| TCCTTGACATCTGTTCTTAC+AGG | + | chr4.1:58539919-58539938 | MS.gene065951:intron | 40.0% | |
| TGGCGAAACTGAAGATAAGA+TGG | - | chr4.1:58540814-58540833 | None:intergenic | 40.0% | |
| TGTGACTATAGACGACTGTT+GGG | + | chr4.1:58539554-58539573 | MS.gene065951:intron | 40.0% | |
| TTATCTTCAGTTTCGCCATG+TGG | + | chr4.1:58540816-58540835 | MS.gene065951:CDS | 40.0% | |
| TTATTCTTTCCAGGAGCAGA+AGG | + | chr4.1:58540608-58540627 | MS.gene065951:intron | 40.0% | |
| TTCTCTCTTCTGTGAAGCAA+GGG | + | chr4.1:58540538-58540557 | MS.gene065951:intron | 40.0% | |
| TTGACATCTGTTCTTACAGG+AGG | + | chr4.1:58539922-58539941 | MS.gene065951:intron | 40.0% | |
| TTGTGACTATAGACGACTGT+TGG | + | chr4.1:58539553-58539572 | MS.gene065951:intron | 40.0% | |
| ! | ACCCCACCAAAATTAAGTTG+TGG | + | chr4.1:58539864-58539883 | MS.gene065951:intron | 40.0% |
| ! | TCTAGAGCAGCAAATTAACG+AGG | + | chr4.1:58539616-58539635 | MS.gene065951:CDS | 40.0% |
| CCGCAACAGGCAATTTGTAA+AGG | + | chr4.1:58539775-58539794 | MS.gene065951:intron | 45.0% | |
| CTATAGACGACTGTTGGGAA+AGG | + | chr4.1:58539559-58539578 | MS.gene065951:intron | 45.0% | |
| CTGTTGGGAAAGGACTTATC+TGG | + | chr4.1:58539569-58539588 | MS.gene065951:CDS | 45.0% | |
| CTTCTCTCTTCTGTGAAGCA+AGG | + | chr4.1:58540537-58540556 | MS.gene065951:intron | 45.0% | |
| CTTCTGTGAAGCAAGGGAAA+GGG | + | chr4.1:58540544-58540563 | MS.gene065951:intron | 45.0% | |
| GATATTTGACAACGCTGGTG+AGG | - | chr4.1:58539713-58539732 | None:intergenic | 45.0% | |
| GATTATCATCCGTGAAGGAG+AGG | + | chr4.1:58539639-58539658 | MS.gene065951:CDS | 45.0% | |
| GGGAAAGGACTTATCTGGAT+TGG | + | chr4.1:58539574-58539593 | MS.gene065951:CDS | 45.0% | |
| GTCAAATATCAGCCATGCCA+CGG | + | chr4.1:58539723-58539742 | MS.gene065951:intron | 45.0% | |
| TCTTCTGTGAAGCAAGGGAA+AGG | + | chr4.1:58540543-58540562 | MS.gene065951:intron | 45.0% | |
| TGGCTGATATTTGACAACGC+TGG | - | chr4.1:58539718-58539737 | None:intergenic | 45.0% | |
| TTGCAGATGGCAGACCATTT+CGG | - | chr4.1:58539831-58539850 | None:intergenic | 45.0% | |
| ! | CAGAATTTGACTTCCGCAAC+AGG | + | chr4.1:58539762-58539781 | MS.gene065951:intron | 45.0% |
| ! | GCAGTGTTATCGATTGCAGA+TGG | - | chr4.1:58539844-58539863 | None:intergenic | 45.0% |
| ! | TCCGAAGGAAGATTCTAGGA+TGG | + | chr4.1:58539386-58539405 | MS.gene065951:CDS | 45.0% |
| ! | TTCTTCAGAGAGTGCATCAG+TGG | + | chr4.1:58539236-58539255 | MS.gene065951:CDS | 45.0% |
| !! | GAGCAGTTTCTAGTCAAGCT+GGG | - | chr4.1:58540880-58540899 | None:intergenic | 45.0% |
| !! | TGAGCAGTTTCTAGTCAAGC+TGG | - | chr4.1:58540881-58540900 | None:intergenic | 45.0% |
| AAGGACCTTCCTCTCCTTCA+CGG | - | chr4.1:58539651-58539670 | None:intergenic | 50.0% | |
| ACTGGAGCAGTCTCGAATAC+AGG | + | chr4.1:58539961-58539980 | MS.gene065951:CDS | 50.0% | |
| ATCATCCGTGAAGGAGAGGA+AGG | + | chr4.1:58539643-58539662 | MS.gene065951:CDS | 50.0% | |
| ATTTGACAACGCTGGTGAGG+TGG | - | chr4.1:58539710-58539729 | None:intergenic | 50.0% | |
| CATGCCACGGCTTATTGCAT+GGG | + | chr4.1:58539736-58539755 | MS.gene065951:intron | 50.0% | |
| CGAGGGATTATCATCCGTGA+AGG | + | chr4.1:58539634-58539653 | MS.gene065951:CDS | 50.0% | |
| GGATTCCAGGCTTTCCGAAA+TGG | + | chr4.1:58539814-58539833 | MS.gene065951:intron | 50.0% | |
| TTTGACAACGCTGGTGAGGT+GGG | - | chr4.1:58539709-58539728 | None:intergenic | 50.0% | |
| ! | TTCAGAGAGTGCATCAGTGG+AGG | + | chr4.1:58539239-58539258 | MS.gene065951:CDS | 50.0% |
| AAAGCCTGGAATCCGCCATG+GGG | - | chr4.1:58539808-58539827 | None:intergenic | 55.0% | |
| CCATGCAATAAGCCGTGGCA+TGG | - | chr4.1:58539738-58539757 | None:intergenic | 55.0% | |
| CCATGCCACGGCTTATTGCA+TGG | + | chr4.1:58539735-58539754 | MS.gene065951:intron | 55.0% | |
| GAAAGCCTGGAATCCGCCAT+GGG | - | chr4.1:58539809-58539828 | None:intergenic | 55.0% | |
| GCAGACCATTTCGGAAAGCC+TGG | - | chr4.1:58539822-58539841 | None:intergenic | 55.0% | |
| TGTAAAGGATGGCAGCCCCA+TGG | + | chr4.1:58539790-58539809 | MS.gene065951:intron | 55.0% | |
| TTGACAACGCTGGTGAGGTG+GGG | - | chr4.1:58539708-58539727 | None:intergenic | 55.0% | |
| AAAGGATGGCAGCCCCATGG+CGG | + | chr4.1:58539793-58539812 | MS.gene065951:intron | 60.0% | |
| CCAGCATAGCCTTCTGCTCC+TGG | - | chr4.1:58540620-58540639 | None:intergenic | 60.0% | |
| CCAGGAGCAGAAGGCTATGC+TGG | + | chr4.1:58540617-58540636 | MS.gene065951:intron | 60.0% | |
| GGAAAGCCTGGAATCCGCCA+TGG | - | chr4.1:58539810-58539829 | None:intergenic | 60.0% | |
| GTCGCCCATGCAATAAGCCG+TGG | - | chr4.1:58539743-58539762 | None:intergenic | 60.0% | |
| TGACAACGCTGGTGAGGTGG+GGG | - | chr4.1:58539707-58539726 | None:intergenic | 60.0% | |
| !! | GCAGCCCCATGGCGGATTCC+AGG | + | chr4.1:58539801-58539820 | MS.gene065951:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 58539197 | 58540938 | 58539197 | ID=MS.gene065951 |
| chr4.1 | mRNA | 58539197 | 58540938 | 58539197 | ID=MS.gene065951.t1;Parent=MS.gene065951 |
| chr4.1 | exon | 58539197 | 58539275 | 58539197 | ID=MS.gene065951.t1.exon1;Parent=MS.gene065951.t1 |
| chr4.1 | CDS | 58539197 | 58539275 | 58539197 | ID=cds.MS.gene065951.t1;Parent=MS.gene065951.t1 |
| chr4.1 | exon | 58539381 | 58539454 | 58539381 | ID=MS.gene065951.t1.exon2;Parent=MS.gene065951.t1 |
| chr4.1 | CDS | 58539381 | 58539454 | 58539381 | ID=cds.MS.gene065951.t1;Parent=MS.gene065951.t1 |
| chr4.1 | exon | 58539565 | 58539664 | 58539565 | ID=MS.gene065951.t1.exon3;Parent=MS.gene065951.t1 |
| chr4.1 | CDS | 58539565 | 58539664 | 58539565 | ID=cds.MS.gene065951.t1;Parent=MS.gene065951.t1 |
| chr4.1 | exon | 58539941 | 58539982 | 58539941 | ID=MS.gene065951.t1.exon4;Parent=MS.gene065951.t1 |
| chr4.1 | CDS | 58539941 | 58539982 | 58539941 | ID=cds.MS.gene065951.t1;Parent=MS.gene065951.t1 |
| chr4.1 | exon | 58540621 | 58540662 | 58540621 | ID=MS.gene065951.t1.exon5;Parent=MS.gene065951.t1 |
| chr4.1 | CDS | 58540621 | 58540662 | 58540621 | ID=cds.MS.gene065951.t1;Parent=MS.gene065951.t1 |
| chr4.1 | exon | 58540766 | 58540938 | 58540766 | ID=MS.gene065951.t1.exon6;Parent=MS.gene065951.t1 |
| chr4.1 | CDS | 58540766 | 58540938 | 58540766 | ID=cds.MS.gene065951.t1;Parent=MS.gene065951.t1 |
| Gene Sequence |
| Protein sequence |
