AlfalfaGEDB | MS.gene066021

M. sativa cultivar XinJiangDaYe / MS.gene066021

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066021.t1	XP_003590815.1	85.2	142	20	1	1	142	1	141	7.60E-59	236.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066021.t1	G7I4T0	85.2	142	20	1	1	142	1	141	5.5e-59	236.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene066021	0.810229	1.28E-50	-1.69E-46
MS.gene051699	MS.gene066021	0.808015	3.81E-50	-1.69E-46
MS.gene053963	MS.gene066021	0.807962	3.91E-50	-1.69E-46
MS.gene056272	MS.gene066021	0.802725	4.90E-49	-1.69E-46
MS.gene056776	MS.gene066021	0.806197	9.24E-50	-1.69E-46
MS.gene058279	MS.gene066021	0.80568	1.19E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066021.t1	MTR_1g075530	85.211	142	20	1	1	142	1	141	1.26e-85	246
MS.gene066021.t1	MTR_1g069640	35.897	156	60	3	13	140	11	154	5.30e-21	83.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066021.t1	AT3G23440	38.686	137	53	4	3	139	1	106	2.52e-22	85.5
MS.gene066021.t1	AT5G44060	40.187	107	50	2	33	139	56	148	3.10e-18	76.3
MS.gene066021.t1	AT1G04000	35.849	106	48	3	35	139	64	150	1.38e-13	64.3

Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGCTTTCTCGTATTGCTGCC+AGG	0.346363	1.2:-58175693	None:intergenic
GGTTCACAATTCCCTTTATG+TGG	0.400824	1.2:-58176013	None:intergenic
TTGACCAAAGGGACTCTACT+TGG	0.410604	1.2:+58175795	MS.gene066021:CDS
AGAAAGGAGCATGAGACTTA+TGG	0.415300	1.2:+58175957	MS.gene066021:CDS
GACGGATGGGACCCACATAA+AGG	0.419954	1.2:+58176001	MS.gene066021:CDS
CTGTCCAAGTAGAGTCCCTT+TGG	0.427421	1.2:-58175799	None:intergenic
AAAGCCTCGGATGAAGAGTT+GGG	0.458598	1.2:+58175921	MS.gene066021:CDS
GACAGAGTCAAAATCAGTTT+CGG	0.462867	1.2:-58175850	None:intergenic
TTGATCATCGTAATCTAAAC+CGG	0.471787	1.2:+58175826	MS.gene066021:CDS
GATGAAGAGTTGGGGCGAGC+TGG	0.476104	1.2:+58175930	MS.gene066021:CDS
GGGGCGAGCTGGAGTGAGAA+AGG	0.488214	1.2:+58175941	MS.gene066021:CDS
CCTTCCAACCGGCTTCTAGC+TGG	0.488781	1.2:+58175753	MS.gene066021:CDS
ACGGATGGGACCCACATAAA+GGG	0.499941	1.2:+58176002	MS.gene066021:CDS
GTGACTCTGACTCATCATCA+TGG	0.501827	1.2:-58175640	None:intergenic
TGAACCCCACACAGCTCTCT+AGG	0.505854	1.2:+58176030	MS.gene066021:CDS
TAAAGCCTCGGATGAAGAGT+TGG	0.507788	1.2:+58175920	MS.gene066021:CDS
ACATTCTGAAGCTGATAAGC+TGG	0.508021	1.2:+58175719	MS.gene066021:CDS
AGTCAAAATCAGTTTCGGTC+CGG	0.512043	1.2:-58175845	None:intergenic
GTTCACAATTCCCTTTATGT+GGG	0.514980	1.2:-58176012	None:intergenic
ATAAGCCGATACCTTCCAAC+CGG	0.524893	1.2:+58175742	MS.gene066021:CDS
CAAGATAGCCAGCTAGAAGC+CGG	0.533977	1.2:-58175761	None:intergenic
TGACTCTGACTCATCATCAT+GGG	0.534674	1.2:-58175639	None:intergenic
GCAAACATTGTGAAGACGGA+TGG	0.548487	1.2:+58175987	MS.gene066021:CDS
ATCCACCTAGAGAGCTGTGT+GGG	0.552677	1.2:-58176035	None:intergenic
AGAAGCCGGTTGGAAGGTAT+CGG	0.561487	1.2:-58175747	None:intergenic
CCAGCTAGAAGCCGGTTGGA+AGG	0.573128	1.2:-58175753	None:intergenic
TCTCGTAGGATGTTGCCACC+TGG	0.581097	1.2:+58175675	MS.gene066021:CDS
ATAGCCAGCTAGAAGCCGGT+TGG	0.585352	1.2:-58175757	None:intergenic
GCATGAGACTTATGGTGAGG+TGG	0.623580	1.2:+58175965	MS.gene066021:CDS
CAAACATTGTGAAGACGGAT+GGG	0.630487	1.2:+58175988	MS.gene066021:CDS
TCGCCCCAACTCTTCATCCG+AGG	0.631269	1.2:-58175925	None:intergenic
TTACTCGGGAGCTAAAGCCT+CGG	0.634204	1.2:+58175908	MS.gene066021:CDS
ACAATCCTCACAATTCTCGT+AGG	0.649237	1.2:+58175661	MS.gene066021:CDS
GGTGGCAAACATTGTGAAGA+CGG	0.666154	1.2:+58175983	MS.gene066021:CDS
AAGCCTCGGATGAAGAGTTG+GGG	0.667128	1.2:+58175922	MS.gene066021:CDS
ACCCCACACAGCTCTCTAGG+TGG	0.680192	1.2:+58176033	MS.gene066021:CDS
TTTCTCGTATTGCTGCCAGG+TGG	0.689552	1.2:-58175690	None:intergenic
GGAGCATGAGACTTATGGTG+AGG	0.701766	1.2:+58175962	MS.gene066021:CDS
TCCACCTAGAGAGCTGTGTG+GGG	0.707423	1.2:-58176034	None:intergenic
AACATCCTACGAGAATTGTG+AGG	0.714756	1.2:-58175666	None:intergenic
AATCCACCTAGAGAGCTGTG+TGG	0.780127	1.2:-58176036	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCATCGTAATCTAAAC+CGG	+	chr1.2:58175826-58175845	MS.gene066021:CDS	30.0%
	GACAGAGTCAAAATCAGTTT+CGG	-	chr1.2:58175853-58175872	None:intergenic	35.0%
	GTTCACAATTCCCTTTATGT+GGG	-	chr1.2:58176015-58176034	None:intergenic	35.0%
!	ACGACAGAGTCAATTTTACT+CGG	+	chr1.2:58175893-58175912	MS.gene066021:CDS	35.0%
!	CTCATGAGTTTTTGACCAAA+GGG	+	chr1.2:58175784-58175803	MS.gene066021:CDS	35.0%
	AACATCCTACGAGAATTGTG+AGG	-	chr1.2:58175669-58175688	None:intergenic	40.0%
	ACAATCCTCACAATTCTCGT+AGG	+	chr1.2:58175661-58175680	MS.gene066021:CDS	40.0%
	AGTCAAAATCAGTTTCGGTC+CGG	-	chr1.2:58175848-58175867	None:intergenic	40.0%
	CAAACATTGTGAAGACGGAT+GGG	+	chr1.2:58175988-58176007	MS.gene066021:CDS	40.0%
	GGTTCACAATTCCCTTTATG+TGG	-	chr1.2:58176016-58176035	None:intergenic	40.0%
!	AGAAAGGAGCATGAGACTTA+TGG	+	chr1.2:58175957-58175976	MS.gene066021:CDS	40.0%
!	CGACAGAGTCAATTTTACTC+GGG	+	chr1.2:58175894-58175913	MS.gene066021:CDS	40.0%
!	GCTCATGAGTTTTTGACCAA+AGG	+	chr1.2:58175783-58175802	MS.gene066021:CDS	40.0%
!!	ACATTCTGAAGCTGATAAGC+TGG	+	chr1.2:58175719-58175738	MS.gene066021:CDS	40.0%
!!!	ACTGATTTTGACTCTGTCGT+TGG	+	chr1.2:58175855-58175874	MS.gene066021:CDS	40.0%
	AAAGCCTCGGATGAAGAGTT+GGG	+	chr1.2:58175921-58175940	MS.gene066021:CDS	45.0%
	ATAAGCCGATACCTTCCAAC+CGG	+	chr1.2:58175742-58175761	MS.gene066021:CDS	45.0%
	GCAAACATTGTGAAGACGGA+TGG	+	chr1.2:58175987-58176006	MS.gene066021:CDS	45.0%
	GGTGGCAAACATTGTGAAGA+CGG	+	chr1.2:58175983-58176002	MS.gene066021:CDS	45.0%
	TAAAGCCTCGGATGAAGAGT+TGG	+	chr1.2:58175920-58175939	MS.gene066021:CDS	45.0%
	TTGACCAAAGGGACTCTACT+TGG	+	chr1.2:58175795-58175814	MS.gene066021:CDS	45.0%
	AAGCCTCGGATGAAGAGTTG+GGG	+	chr1.2:58175922-58175941	MS.gene066021:CDS	50.0%
	AATCCACCTAGAGAGCTGTG+TGG	-	chr1.2:58176039-58176058	None:intergenic	50.0%
	ACGGATGGGACCCACATAAA+GGG	+	chr1.2:58176002-58176021	MS.gene066021:CDS	50.0%
	ATCCACCTAGAGAGCTGTGT+GGG	-	chr1.2:58176038-58176057	None:intergenic	50.0%
	CAAGATAGCCAGCTAGAAGC+CGG	-	chr1.2:58175764-58175783	None:intergenic	50.0%
	CTGTCCAAGTAGAGTCCCTT+TGG	-	chr1.2:58175802-58175821	None:intergenic	50.0%
	GCATGAGACTTATGGTGAGG+TGG	+	chr1.2:58175965-58175984	MS.gene066021:CDS	50.0%
	GGAGCATGAGACTTATGGTG+AGG	+	chr1.2:58175962-58175981	MS.gene066021:CDS	50.0%
	TTACTCGGGAGCTAAAGCCT+CGG	+	chr1.2:58175908-58175927	MS.gene066021:CDS	50.0%
	TTTCTCGTATTGCTGCCAGG+TGG	-	chr1.2:58175693-58175712	None:intergenic	50.0%
!	AGAAGCCGGTTGGAAGGTAT+CGG	-	chr1.2:58175750-58175769	None:intergenic	50.0%
	ATAGCCAGCTAGAAGCCGGT+TGG	-	chr1.2:58175760-58175779	None:intergenic	55.0%
	CGCTTTCTCGTATTGCTGCC+AGG	-	chr1.2:58175696-58175715	None:intergenic	55.0%
	GACGGATGGGACCCACATAA+AGG	+	chr1.2:58176001-58176020	MS.gene066021:CDS	55.0%
	TCCACCTAGAGAGCTGTGTG+GGG	-	chr1.2:58176037-58176056	None:intergenic	55.0%
	TCTCGTAGGATGTTGCCACC+TGG	+	chr1.2:58175675-58175694	MS.gene066021:CDS	55.0%
	TGAACCCCACACAGCTCTCT+AGG	+	chr1.2:58176030-58176049	MS.gene066021:CDS	55.0%
	ACCCCACACAGCTCTCTAGG+TGG	+	chr1.2:58176033-58176052	MS.gene066021:CDS	60.0%
	CCAGCTAGAAGCCGGTTGGA+AGG	-	chr1.2:58175756-58175775	None:intergenic	60.0%
	GATGAAGAGTTGGGGCGAGC+TGG	+	chr1.2:58175930-58175949	MS.gene066021:CDS	60.0%
	TCGCCCCAACTCTTCATCCG+AGG	-	chr1.2:58175928-58175947	None:intergenic	60.0%
!	CCTTCCAACCGGCTTCTAGC+TGG	+	chr1.2:58175753-58175772	MS.gene066021:CDS	60.0%
	GGGGCGAGCTGGAGTGAGAA+AGG	+	chr1.2:58175941-58175960	MS.gene066021:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	58175642	58176070	58175642	ID=MS.gene066021
chr1.2	mRNA	58175642	58176070	58175642	ID=MS.gene066021.t1;Parent=MS.gene066021
chr1.2	exon	58175642	58176070	58175642	ID=MS.gene066021.t1.exon1;Parent=MS.gene066021.t1
chr1.2	CDS	58175642	58176070	58175642	ID=cds.MS.gene066021.t1;Parent=MS.gene066021.t1

Gene Sequence

>MS.gene066021

ATGATGATGAGTCAGAGTCACAATCCTCACAATTCTCGTAGGATGTTGCCACCTGGCAGCAATACGAGAAAGCGCAAACATTCTGAAGCTGATAAGCTGGATAAGCCGATACCTTCCAACCGGCTTCTAGCTGGCTATCTTGCTCATGAGTTTTTGACCAAAGGGACTCTACTTGGACAGAAGTTTGATCATCGTAATCTAAACCGGACCGAAACTGATTTTGACTCTGTCGTTGGCTCAGCTGAACAGCAACGACAGAGTCAATTTTACTCGGGAGCTAAAGCCTCGGATGAAGAGTTGGGGCGAGCTGGAGTGAGAAAGGAGCATGAGACTTATGGTGAGGTGGCAAACATTGTGAAGACGGATGGGACCCACATAAAGGGAATTGTGAACCCCACACAGCTCTCTAGGTGGATTCACAAATCATGA

Protein sequence

>MS.gene066021.t1

MMMSQSHNPHNSRRMLPPGSNTRKRKHSEADKLDKPIPSNRLLAGYLAHEFLTKGTLLGQKFDHRNLNRTETDFDSVVGSAEQQRQSQFYSGAKASDEELGRAGVRKEHETYGEVANIVKTDGTHIKGIVNPTQLSRWIHKS