AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066022

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066022.t1 MTR_1g075540 93.662 142 9 0 1 142 1 142 2.26e-95 281
MS.gene066022.t1 MTR_1g075540 93.662 142 9 0 1 142 1 142 2.88e-95 280
MS.gene066022.t1 MTR_2g023850 54.930 142 64 0 1 142 1 142 3.54e-49 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066022.t1 AT4G14305 73.239 142 38 0 1 142 1 142 1.01e-76 233
MS.gene066022.t1 AT4G14305 72.414 116 32 0 27 142 15 130 1.50e-60 191
MS.gene066022.t1 AT4G04470 59.231 130 53 0 10 139 12 141 1.47e-51 169
MS.gene066022.t1 AT4G04470 59.231 130 53 0 10 139 12 141 1.47e-51 169

Find 87 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGTTGCACAGAAGCTTTC+TGG 0.182029 1.2:-58212192 MS.gene066022:CDS
TTTATTGCTGTAACAAATTC+TGG 0.185106 1.2:-58209478 MS.gene066022:CDS
AAAGTTTATCCATCAATAAA+TGG 0.290399 1.2:+58211732 None:intergenic
GGCACTTCCCACCTCCGTTC+AGG 0.305018 1.2:-58209235 MS.gene066022:CDS
TTCTTTGCTGATTCCAGATC+TGG 0.312941 1.2:-58209185 MS.gene066022:CDS
AGAATTTGTTACAGCAATAA+AGG 0.313308 1.2:+58209480 None:intergenic
GGCGGCGGCAGTGGCTGTTC+TGG 0.313368 1.2:+58209432 None:intergenic
GTGGCTGTTCTGGCCGGAAA+AGG 0.320658 1.2:+58209442 None:intergenic
GTGCCTGGAAAGTGCGATTC+TGG 0.324970 1.2:+58209411 None:intergenic
CGTCCGTATCGGCCTGCTTC+CGG 0.336358 1.2:-58209352 MS.gene066022:CDS
TGCGATTCTGGCGGCGGCAG+TGG 0.352418 1.2:+58209423 None:intergenic
CCGCCAGAATCGCACTTTCC+AGG 0.359728 1.2:-58209414 MS.gene066022:CDS
ACATTATCTCCATTTATTGA+TGG 0.376713 1.2:-58211741 MS.gene066022:CDS
TTCGCCTATGCAGGACCCTT+TGG 0.383790 1.2:-58211764 MS.gene066022:CDS
GCACTTTCCAGGCACTATTC+CGG 0.395344 1.2:-58209403 MS.gene066022:CDS
GTCAACAGTCTTATGCTGTT+TGG 0.407017 1.2:-58210089 MS.gene066022:CDS
GGATGGTGGTGTTATCTTGC+CGG 0.411098 1.2:+58209384 None:intergenic
TATTATGGCTTGATTATTGA+AGG 0.419845 1.2:-58211233 MS.gene066022:intron
AACTATTCAAATTGTTCCAT+TGG 0.421331 1.2:+58210066 None:intergenic
CAATGAAACTGTTGCTAAGA+AGG 0.429878 1.2:-58211693 MS.gene066022:intron
GCGGGCGCGTTGCGACGTTT+CGG 0.436145 1.2:+58209318 None:intergenic
GCACTTCCCACCTCCGTTCA+GGG 0.437899 1.2:-58209234 MS.gene066022:CDS
GTGTAGGGTTGTCTCTCAAT+AGG 0.445725 1.2:+58209586 None:intergenic
TGGCTGTTCTGGCCGGAAAA+GGG 0.445897 1.2:+58209443 None:intergenic
GGCAAACCGTGTTTCATCTC+CGG 0.447688 1.2:-58209513 MS.gene066022:CDS
CTCTATGGTTTCGCCTATGC+AGG 0.448355 1.2:-58211773 MS.gene066022:CDS
TCAGCAAAGAATTCCGAGTC+TGG 0.456990 1.2:+58209197 None:intergenic
AGCGGCACCCTGAACGGAGG+TGG 0.459182 1.2:+58209227 None:intergenic
ATTACTTCATCAGTTCTAGC+TGG 0.466658 1.2:-58212225 MS.gene066022:CDS
GCCGCTGCTCTCGCCAGACT+CGG 0.470170 1.2:-58209210 MS.gene066022:CDS
CGGCGCGTGGGAGTTTGCGC+TGG 0.475351 1.2:+58209278 None:intergenic
CTACCCTACTGTTCAGTTGA+CGG 0.475476 1.2:-58210130 MS.gene066022:CDS
GGAAAGTGCGATTCTGGCGG+CGG 0.478675 1.2:+58209417 None:intergenic
GTCTTATGCTGTTTGGCCAA+TGG 0.482021 1.2:-58210082 MS.gene066022:CDS
GCGGCAGTGGCTGTTCTGGC+CGG 0.492506 1.2:+58209436 None:intergenic
AGTTGACGGCGTGGAAGGTA+TGG 0.495019 1.2:-58210116 MS.gene066022:CDS
CTGACAAAGTCAACGATGCT+TGG 0.498624 1.2:-58212388 MS.gene066022:CDS
AGGACATGCAGTGGGTCCAC+CGG 0.501300 1.2:+58209554 None:intergenic
TGGACCCACTGCATGTCCTC+CGG 0.502547 1.2:-58209550 MS.gene066022:CDS
ACAGCTTTGTTGGTTCATGC+TGG 0.506633 1.2:-58209937 MS.gene066022:intron
TTTCCGGAAGCAGGCCGATA+CGG 0.508572 1.2:+58209349 None:intergenic
GTTGGTTTGCAGGAAGACCT+TGG 0.511703 1.2:-58210182 MS.gene066022:intron
AAATGGAGATAATGTCCAAA+GGG 0.531298 1.2:+58211749 None:intergenic
AGCAAATAACTAGTTCTCCA+TGG 0.534969 1.2:-58211277 MS.gene066022:CDS
CTAAGAAGCTTCAGTTCAGA+AGG 0.536401 1.2:-58212167 MS.gene066022:CDS
GAGAACTAGTTATTTGCTCA+AGG 0.536726 1.2:+58211282 None:intergenic
GCGCGTTGCGACGTTTCGGA+TGG 0.537025 1.2:+58209322 None:intergenic
TATCTTGCCGGAATAGTGCC+TGG 0.542579 1.2:+58209396 None:intergenic
AACCCTACACCGTGATTACC+CGG 0.543148 1.2:-58209573 MS.gene066022:CDS
TGTTCAGTTGACGGCGTGGA+AGG 0.546675 1.2:-58210121 MS.gene066022:CDS
GGACATGCAGTGGGTCCACC+GGG 0.548671 1.2:+58209555 None:intergenic
GAGAGCAGCGGCACCCTGAA+CGG 0.550318 1.2:+58209221 None:intergenic
ATCGTTGACTTTGTCAGACA+TGG 0.551719 1.2:+58212394 None:intergenic
CCACCGGGTAATCACGGTGT+AGG 0.553165 1.2:+58209570 None:intergenic
CCGCTTCGTCTCGTCCGTAT+CGG 0.555936 1.2:-58209363 MS.gene066022:CDS
AACTAGAGCACGTATTTCCG+TGG 0.556675 1.2:+58209142 None:intergenic
CACCGGGTAATCACGGTGTA+GGG 0.561996 1.2:+58209571 None:intergenic
CACGCCGTCAACTGAACAGT+AGG 0.562833 1.2:+58210126 None:intergenic
GAGGATGAAGCTTAAGCTGA+AGG 0.563374 1.2:+58212354 None:intergenic
GTGGGTCCACCGGGTAATCA+CGG 0.571604 1.2:+58209564 None:intergenic
TTGTTCATGACTATATTCCA+AGG 0.574186 1.2:+58210165 None:intergenic
ATGTCCAAAGGGTCCTGCAT+AGG 0.576071 1.2:+58211760 None:intergenic
TGTTACAGCAATAAAGGAGG+GGG 0.576189 1.2:+58209486 None:intergenic
GAGAGAAAAGAGACGCGTGC+GGG 0.576209 1.2:+58209300 None:intergenic
GTGGGAAGTGCCGTCACGCG+CGG 0.577634 1.2:+58209246 None:intergenic
GCGGCACCCTGAACGGAGGT+GGG 0.584124 1.2:+58209228 None:intergenic
TCCGAGTCTGGCGAGAGCAG+CGG 0.586196 1.2:+58209209 None:intergenic
GCTTCATCCTCTCAGAACCA+AGG 0.597074 1.2:-58212342 MS.gene066022:intron
TTTGTTACAGCAATAAAGGA+GGG 0.601768 1.2:+58209484 None:intergenic
ATTTGTTACAGCAATAAAGG+AGG 0.609053 1.2:+58209483 None:intergenic
GGAAGTGCCGTCACGCGCGG+TGG 0.612522 1.2:+58209249 None:intergenic
CCTGGAAAGTGCGATTCTGG+CGG 0.613637 1.2:+58209414 None:intergenic
TAAATGGAGATAATGTCCAA+AGG 0.616169 1.2:+58211748 None:intergenic
GGACGAGACGAAGCGGATGG+TGG 0.633886 1.2:+58209370 None:intergenic
CCGATACGGACGAGACGAAG+CGG 0.650773 1.2:+58209363 None:intergenic
TACGGACGAGACGAAGCGGA+TGG 0.652123 1.2:+58209367 None:intergenic
CTACTGTTCAGTTGACGGCG+TGG 0.655022 1.2:-58210125 MS.gene066022:CDS
AATGAAACCTTGGTTCTGAG+AGG 0.655224 1.2:+58212335 None:intergenic
TTGTTACAGCAATAAAGGAG+GGG 0.656342 1.2:+58209485 None:intergenic
ACGCCGTCAACTGAACAGTA+GGG 0.658501 1.2:+58210127 None:intergenic
TCTCACCGGAGGACATGCAG+TGG 0.664232 1.2:+58209545 None:intergenic
GGAGAGAAAAGAGACGCGTG+CGG 0.666973 1.2:+58209299 None:intergenic
AGCAGCGGCACCCTGAACGG+AGG 0.670997 1.2:+58209224 None:intergenic
CAAAGCTGTGAAATAAAACA+CGG 0.671747 1.2:+58209951 None:intergenic
CTCACCGGAGGACATGCAGT+GGG 0.672600 1.2:+58209546 None:intergenic
GGCAAACCGGAGATGAAACA+CGG 0.680583 1.2:+58209507 None:intergenic
CCTACACCGTGATTACCCGG+TGG 0.755491 1.2:-58209570 MS.gene066022:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAACATTTAAAATTATTAC+TGG + chr1.2:58209519-58209538 None:intergenic 10.0%
!! ATAATGATTATTGTAAACTT+AGG + chr1.2:58210653-58210672 None:intergenic 15.0%
!! ATGATTAAGATATTATCAAT+AGG + chr1.2:58211251-58211270 None:intergenic 15.0%
!! ATTTAGTGTTATATTGTATT+GGG + chr1.2:58209995-58210014 None:intergenic 15.0%
!! CAATTAATACTAAATATTAG+AGG + chr1.2:58210515-58210534 None:intergenic 15.0%
!! GAAAACATTTATAGAATAAA+AGG - chr1.2:58210807-58210826 MS.gene066022:intron 15.0%
!! TAATGATTATTGTAAACTTA+GGG + chr1.2:58210652-58210671 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + chr1.2:58209996-58210015 None:intergenic 15.0%
!! TCAAAATTATCAATGTTTAT+AGG - chr1.2:58210615-58210634 MS.gene066022:intron 15.0%
!!! AAGAAAAGTGTATAAATAAA+GGG + chr1.2:58210060-58210079 None:intergenic 15.0%
!!! TAAGAAAAGTGTATAAATAA+AGG + chr1.2:58210061-58210080 None:intergenic 15.0%
!! AAAATAGTAAAATGAAACCT+TGG + chr1.2:58209214-58209233 None:intergenic 20.0%
!! AAAGTGGAATATATTGTATT+GGG + chr1.2:58210036-58210055 None:intergenic 20.0%
!! AAAGTTTATCCATCAATAAA+TGG + chr1.2:58209807-58209826 None:intergenic 20.0%
!! AATTTAAGAAAGTAAGTACT+TGG + chr1.2:58210886-58210905 None:intergenic 20.0%
!! GAATGAAATATCATATTACT+AGG - chr1.2:58210227-58210246 MS.gene066022:intron 20.0%
!! TCATTATTTACAAGTCAATA+TGG - chr1.2:58210666-58210685 MS.gene066022:intron 20.0%
!! TTAACATTGAATTTAATTCC+CGG - chr1.2:58210924-58210943 MS.gene066022:intron 20.0%
!! TTAAGATATTATCAATAGGT+TGG + chr1.2:58211247-58211266 None:intergenic 20.0%
!!! ACAATGCTTAATCATTATTT+TGG + chr1.2:58211699-58211718 None:intergenic 20.0%
!!! ACATTGATAATTTTGAACTT+CGG + chr1.2:58210611-58210630 None:intergenic 20.0%
!!! AGAAAAGTGTATAAATAAAG+GGG + chr1.2:58210059-58210078 None:intergenic 20.0%
!!! CAATTTGAATAGTTAGTTTT+TGG - chr1.2:58211479-58211498 MS.gene066022:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + chr1.2:58209926-58209945 None:intergenic 20.0%
!!! TTTTTGTTCATGATGTATTA+TGG - chr1.2:58210288-58210307 MS.gene066022:intron 20.0%
! AACTATTCAAATTGTTCCAT+TGG + chr1.2:58211473-58211492 None:intergenic 25.0%
! ACATTATCTCCATTTATTGA+TGG - chr1.2:58209795-58209814 MS.gene066022:intron 25.0%
! CTGTAAATATATCATAGCTT+AGG - chr1.2:58211820-58211839 MS.gene066022:intron 25.0%
! GAAAGTGGAATATATTGTAT+TGG + chr1.2:58210037-58210056 None:intergenic 25.0%
! TATATAGAAGTAAGTAGCTT+GGG + chr1.2:58211847-58211866 None:intergenic 25.0%
! TATTATGGCTTGATTATTGA+AGG - chr1.2:58210303-58210322 MS.gene066022:intron 25.0%
! TTACCTTACAAGTATATGAA+GGG - chr1.2:58210852-58210871 MS.gene066022:intron 25.0%
! TTATATAGAAGTAAGTAGCT+TGG + chr1.2:58211848-58211867 None:intergenic 25.0%
! TTGTTCGCAATATCATATTA+AGG + chr1.2:58210780-58210799 None:intergenic 25.0%
! TTTATTGCTGTAACAAATTC+TGG - chr1.2:58212058-58212077 MS.gene066022:intron 25.0%
!! AGTTTGATAGTAGTTGAATT+TGG - chr1.2:58209703-58209722 MS.gene066022:intron 25.0%
!! TAGATGAAGTTTAAGCTTTT+TGG - chr1.2:58211164-58211183 MS.gene066022:intron 25.0%
!! TGAAATGAGTCAGTATTTTT+GGG + chr1.2:58209948-58209967 None:intergenic 25.0%
!! TTGAAATGAGTCAGTATTTT+TGG + chr1.2:58209949-58209968 None:intergenic 25.0%
!! TTTTCATAAAACAAGTACCA+GGG + chr1.2:58210199-58210218 None:intergenic 25.0%
!! TTTTTCATAAAACAAGTACC+AGG + chr1.2:58210200-58210219 None:intergenic 25.0%
!!! AGAATTTGTTACAGCAATAA+AGG + chr1.2:58212059-58212078 None:intergenic 25.0%
!!! ATTGATGGATAAACTTTTCA+AGG - chr1.2:58209810-58209829 MS.gene066022:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + chr1.2:58209927-58209946 None:intergenic 25.0%
!!! TTGAATAGTTAGTTTTTGGA+AGG - chr1.2:58211483-58211502 MS.gene066022:intron 25.0%
!!! TTGATGGATAAACTTTTCAA+GGG - chr1.2:58209811-58209830 MS.gene066022:intron 25.0%
!!! TTGTTTTTAGTTTTGATCCA+CGG - chr1.2:58212377-58212396 MS.gene066022:CDS 25.0%
AAATGGAGATAATGTCCAAA+GGG + chr1.2:58209790-58209809 None:intergenic 30.0%
AACCAAACTGAAATAGTGAA+GGG - chr1.2:58211053-58211072 MS.gene066022:intron 30.0%
ATATAGAAGTAAGTAGCTTG+GGG + chr1.2:58211846-58211865 None:intergenic 30.0%
ATGAACAAAAAGTTGTTCCA+TGG + chr1.2:58210279-58210298 None:intergenic 30.0%
CAAAGCTGTGAAATAAAACA+CGG + chr1.2:58211588-58211607 None:intergenic 30.0%
CTATTTATCCCTATAACCAA+AGG + chr1.2:58210137-58210156 None:intergenic 30.0%
CTTACCTTACAAGTATATGA+AGG - chr1.2:58210851-58210870 MS.gene066022:intron 30.0%
GATAGTAGTTGAATTTGGAT+GGG - chr1.2:58209708-58209727 MS.gene066022:intron 30.0%
TAAATGGAGATAATGTCCAA+AGG + chr1.2:58209791-58209810 None:intergenic 30.0%
TAGTATGTCCTTTGGTTATA+GGG - chr1.2:58210126-58210145 MS.gene066022:CDS 30.0%
TGATAGTAGTTGAATTTGGA+TGG - chr1.2:58209707-58209726 MS.gene066022:intron 30.0%
TGCTTTGTAATTAAAGTGTG+AGG - chr1.2:58211332-58211351 MS.gene066022:intron 30.0%
TGTATAAATAAAGGGGAAAG+TGG + chr1.2:58210052-58210071 None:intergenic 30.0%
TTCTGCTTAATTCCTAACTT+TGG - chr1.2:58210543-58210562 MS.gene066022:intron 30.0%
TTGTAATTAAAGTGTGAGGT+TGG - chr1.2:58211336-58211355 MS.gene066022:intron 30.0%
TTGTTCATGACTATATTCCA+AGG + chr1.2:58211374-58211393 None:intergenic 30.0%
TTTGTTACAGCAATAAAGGA+GGG + chr1.2:58212055-58212074 None:intergenic 30.0%
! AAAAGTTTTGCACCGAAATT+TGG + chr1.2:58210739-58210758 None:intergenic 30.0%
! AAAGTTTTGCACCGAAATTT+GGG + chr1.2:58210738-58210757 None:intergenic 30.0%
! ATTTGTTACAGCAATAAAGG+AGG + chr1.2:58212056-58212075 None:intergenic 30.0%
! CTTTTCTCTCTTATTCAACA+TGG - chr1.2:58211919-58211938 MS.gene066022:intron 30.0%
! GAATGTCAATTCTGTTTTCT+TGG + chr1.2:58210493-58210512 None:intergenic 30.0%
! TTAATTCCTAACTTTGGTTC+TGG - chr1.2:58210549-58210568 MS.gene066022:intron 30.0%
!! CAAATTATTTTGCAGCTCTA+TGG - chr1.2:58209748-58209767 MS.gene066022:intron 30.0%
!! TGATGGATAAACTTTTCAAG+GGG - chr1.2:58209812-58209831 MS.gene066022:intron 30.0%
!! TGTGTGTGTTTTGTTATGTT+AGG - chr1.2:58209286-58209305 MS.gene066022:CDS 30.0%
!!! GTTTTATTTCACAGCTTTGT+TGG - chr1.2:58211589-58211608 MS.gene066022:intron 30.0%
!!! GTTTTTTGAACATGCAGTTT+TGG - chr1.2:58211518-58211537 MS.gene066022:intron 30.0%
AATAAAACACGGAACTGCAA+AGG + chr1.2:58211577-58211596 None:intergenic 35.0%
ACATTGAATTTAATTCCCGG+AGG - chr1.2:58210927-58210946 MS.gene066022:intron 35.0%
AGAAAATGGGTCAGTTATTC+CGG + chr1.2:58209913-58209932 None:intergenic 35.0%
AGACCCTTCATATACTTGTA+AGG + chr1.2:58210858-58210877 None:intergenic 35.0%
AGACCTTCTATCCCAAATTT+CGG - chr1.2:58210724-58210743 MS.gene066022:intron 35.0%
ATAGTACCAGAACCAAAGTT+AGG + chr1.2:58210558-58210577 None:intergenic 35.0%
CAAAGTAGGTCAATTATACC+GGG - chr1.2:58210096-58210115 MS.gene066022:CDS 35.0%
CAATGAAACTGTTGCTAAGA+AGG - chr1.2:58209843-58209862 MS.gene066022:intron 35.0%
CCAAAGTAGGTCAATTATAC+CGG - chr1.2:58210095-58210114 MS.gene066022:CDS 35.0%
CTAAAAACAAAGACCAGATC+TGG + chr1.2:58212367-58212386 None:intergenic 35.0%
GAACCAAACTGAAATAGTGA+AGG - chr1.2:58211052-58211071 MS.gene066022:intron 35.0%
GAGAACTAGTTATTTGCTCA+AGG + chr1.2:58210257-58210276 None:intergenic 35.0%
GCTTACTCTCTAAGTTACTT+AGG - chr1.2:58211771-58211790 MS.gene066022:CDS 35.0%
GTAGTATGTCCTTTGGTTAT+AGG - chr1.2:58210125-58210144 MS.gene066022:CDS 35.0%
TCTGTGTGAAAAACCAAAGT+AGG - chr1.2:58210082-58210101 MS.gene066022:CDS 35.0%
TTGCCGAAAAAAATTCTCAC+CGG + chr1.2:58212008-58212027 None:intergenic 35.0%
TTGTTACAGCAATAAAGGAG+GGG + chr1.2:58212054-58212073 None:intergenic 35.0%
! AGCAAATAACTAGTTCTCCA+TGG - chr1.2:58210259-58210278 MS.gene066022:intron 35.0%
! ATGCCTCCAATCAAAAGTAT+CGG - chr1.2:58209576-58209595 MS.gene066022:CDS 35.0%
! ATTACTTCATCAGTTCTAGC+TGG - chr1.2:58209311-58209330 MS.gene066022:CDS 35.0%
! ATTCTGTTTTCTTGGTAGAG+AGG + chr1.2:58210485-58210504 None:intergenic 35.0%
! CAGAAGGATTCTTCTTTTCA+TGG - chr1.2:58209385-58209404 MS.gene066022:CDS 35.0%
! GTATTTTGTGTTGTGTGATC+AGG - chr1.2:58210156-58210175 MS.gene066022:CDS 35.0%
! TAGGTTGGAATTGTTTCATG+AGG + chr1.2:58211232-58211251 None:intergenic 35.0%
!! AAACTTTTCAAGGGGAAGAA+AGG - chr1.2:58209820-58209839 MS.gene066022:intron 35.0%
!!! ATAGCACCGATACTTTTGAT+TGG + chr1.2:58209585-58209604 None:intergenic 35.0%
CCGGTATAATTGACCTACTT+TGG + chr1.2:58210098-58210117 None:intergenic 40.0%
CTAAGAAGCTTCAGTTCAGA+AGG - chr1.2:58209369-58209388 MS.gene066022:CDS 40.0%
GAAAATGGGTCAGTTATTCC+GGG + chr1.2:58209912-58209931 None:intergenic 40.0%
GACCCTTCACTATTTCAGTT+TGG + chr1.2:58211058-58211077 None:intergenic 40.0%
TAAAACAAGTACCAGGGCTA+AGG + chr1.2:58210193-58210212 None:intergenic 40.0%
TAATTCACCCAACCAACGAT+AGG + chr1.2:58211544-58211563 None:intergenic 40.0%
TACAAAGCACAATGCAGTGA+AGG + chr1.2:58211321-58211340 None:intergenic 40.0%
TGTTACAGCAATAAAGGAGG+GGG + chr1.2:58212053-58212072 None:intergenic 40.0%
TTCCTTATATTGCTCTCCTC+CGG + chr1.2:58210946-58210965 None:intergenic 40.0%
! GTCAACAGTCTTATGCTGTT+TGG - chr1.2:58211447-58211466 MS.gene066022:intron 40.0%
! TGTTTTCTTGGTAGAGAGGT+GGG + chr1.2:58210481-58210500 None:intergenic 40.0%
! TTCTATGTTTTCCTTAGCCC+TGG - chr1.2:58210179-58210198 MS.gene066022:intron 40.0%
!! AATGAAACCTTGGTTCTGAG+AGG + chr1.2:58209204-58209223 None:intergenic 40.0%
!! CTTTTGATTGGAGGCATGTT+TGG + chr1.2:58209573-58209592 None:intergenic 40.0%
!! TTCTTTGCTGATTCCAGATC+TGG - chr1.2:58212351-58212370 MS.gene066022:CDS 40.0%
!!! CCTCCGGTGAGAATTTTTTT+CGG - chr1.2:58212002-58212021 MS.gene066022:intron 40.0%
CCGAAAAAAATTCTCACCGG+AGG + chr1.2:58212005-58212024 None:intergenic 45.0%
CTACCCTACTGTTCAGTTGA+CGG - chr1.2:58211406-58211425 MS.gene066022:intron 45.0%
CTGACAAAGTCAACGATGCT+TGG - chr1.2:58209148-58209167 MS.gene066022:CDS 45.0%
GAGGATGAAGCTTAAGCTGA+AGG + chr1.2:58209185-58209204 None:intergenic 45.0%
GCGAACATCAGACACAACAA+TGG - chr1.2:58209474-58209493 MS.gene066022:CDS 45.0%
GTAGGTCAATTATACCGGGA+CGG - chr1.2:58210100-58210119 MS.gene066022:CDS 45.0%
GTGTAGGGTTGTCTCTCAAT+AGG + chr1.2:58211953-58211972 None:intergenic 45.0%
TCAGCAAAGAATTCCGAGTC+TGG + chr1.2:58212342-58212361 None:intergenic 45.0%
TCCTTATATTGCTCTCCTCC+GGG + chr1.2:58210945-58210964 None:intergenic 45.0%
TGTTGCGCTATTCAGACACA+CGG - chr1.2:58211877-58211896 MS.gene066022:intron 45.0%
! CGTTTCGGATGGTGATTTTC+CGG + chr1.2:58212206-58212225 None:intergenic 45.0%
! CTGTTTTCTTGGTAGAGAGG+TGG + chr1.2:58210482-58210501 None:intergenic 45.0%
! GCACCGAAATTTGGGATAGA+AGG + chr1.2:58210730-58210749 None:intergenic 45.0%
! GGAACTGCAAAGGCATATAC+TGG + chr1.2:58211567-58211586 None:intergenic 45.0%
! GTCTTATGCTGTTTGGCCAA+TGG - chr1.2:58211454-58211473 MS.gene066022:intron 45.0%
! TGCTCGTTTGAACCCTTTTC+CGG - chr1.2:58212081-58212100 MS.gene066022:intron 45.0%
!! AAAGTGTGAGGTTGGTTTGC+AGG - chr1.2:58211344-58211363 MS.gene066022:intron 45.0%
!! ACAGCTTTGTTGGTTCATGC+TGG - chr1.2:58211599-58211618 MS.gene066022:intron 45.0%
!! AGTTGAATTTGGATGGGCTG+TGG - chr1.2:58209714-58209733 MS.gene066022:intron 45.0%
!! AGTTTTGGCCTATCGTTGGT+TGG - chr1.2:58211533-58211552 MS.gene066022:intron 45.0%
!! ATGCAGTTTTGGCCTATCGT+TGG - chr1.2:58211529-58211548 MS.gene066022:intron 45.0%
!! GCACCGATACTTTTGATTGG+AGG + chr1.2:58209582-58209601 None:intergenic 45.0%
!! GTTTTGGCCTATCGTTGGTT+GGG - chr1.2:58211534-58211553 MS.gene066022:intron 45.0%
AACCCTACACCGTGATTACC+CGG - chr1.2:58211963-58211982 MS.gene066022:intron 50.0%
ACGCCGTCAACTGAACAGTA+GGG + chr1.2:58211412-58211431 None:intergenic 50.0%
ATGGGTCAGTTATTCCGGGA+CGG + chr1.2:58209908-58209927 None:intergenic 50.0%
ATGTCCAAAGGGTCCTGCAT+AGG + chr1.2:58209779-58209798 None:intergenic 50.0%
CAATAAAGGAGGGGGCAAAC+CGG + chr1.2:58212045-58212064 None:intergenic 50.0%
CTCTATGGTTTCGCCTATGC+AGG - chr1.2:58209763-58209782 MS.gene066022:intron 50.0%
GACGGAGGTAGTATGTCCTT+TGG - chr1.2:58210118-58210137 MS.gene066022:CDS 50.0%
GCACTTTCCAGGCACTATTC+CGG - chr1.2:58212133-58212152 MS.gene066022:intron 50.0%
GCTGTTGCACAGAAGCTTTC+TGG - chr1.2:58209344-58209363 MS.gene066022:CDS 50.0%
GCTTCATCCTCTCAGAACCA+AGG - chr1.2:58209194-58209213 MS.gene066022:CDS 50.0%
GGCAAACCGGAGATGAAACA+CGG + chr1.2:58212032-58212051 None:intergenic 50.0%
GGCAAACCGTGTTTCATCTC+CGG - chr1.2:58212023-58212042 MS.gene066022:intron 50.0%
TATCTTGCCGGAATAGTGCC+TGG + chr1.2:58212143-58212162 None:intergenic 50.0%
TCCCGGAGGAGAGCAATATA+AGG - chr1.2:58210941-58210960 MS.gene066022:intron 50.0%
! GATGGTGATTTTCCGGAAGC+AGG + chr1.2:58212199-58212218 None:intergenic 50.0%
!! GGATGGTGGTGTTATCTTGC+CGG + chr1.2:58212155-58212174 None:intergenic 50.0%
!! GGTGGGTTACACAACACAAG+AGG + chr1.2:58210464-58210483 None:intergenic 50.0%
!! GTTGGTTTGCAGGAAGACCT+TGG - chr1.2:58211354-58211373 MS.gene066022:intron 50.0%
!! TTTGGTGCTCGACACGACAA+TGG + chr1.2:58209555-58209574 None:intergenic 50.0%
AGGACATACTACCTCCGTCC+CGG + chr1.2:58210117-58210136 None:intergenic 55.0%
AGTTGACGGCGTGGAAGGTA+TGG - chr1.2:58211420-58211439 MS.gene066022:intron 55.0%
CACCGGGTAATCACGGTGTA+GGG + chr1.2:58211968-58211987 None:intergenic 55.0%
CACGCCGTCAACTGAACAGT+AGG + chr1.2:58211413-58211432 None:intergenic 55.0%
CTACTGTTCAGTTGACGGCG+TGG - chr1.2:58211411-58211430 MS.gene066022:intron 55.0%
GAGAGAAAAGAGACGCGTGC+GGG + chr1.2:58212239-58212258 None:intergenic 55.0%
GGAGAGAAAAGAGACGCGTG+CGG + chr1.2:58212240-58212259 None:intergenic 55.0%
GGTCAATTATACCGGGACGG+AGG - chr1.2:58210103-58210122 MS.gene066022:CDS 55.0%
GTCAGTTATTCCGGGACGGA+GGG + chr1.2:58209904-58209923 None:intergenic 55.0%
TGGCTGTTCTGGCCGGAAAA+GGG + chr1.2:58212096-58212115 None:intergenic 55.0%
TGTTCAGTTGACGGCGTGGA+AGG - chr1.2:58211415-58211434 MS.gene066022:intron 55.0%
TTCGCCTATGCAGGACCCTT+TGG - chr1.2:58209772-58209791 MS.gene066022:intron 55.0%
TTTCCGGAAGCAGGCCGATA+CGG + chr1.2:58212190-58212209 None:intergenic 55.0%
! CCTGGAAAGTGCGATTCTGG+CGG + chr1.2:58212125-58212144 None:intergenic 55.0%
! GTGCCTGGAAAGTGCGATTC+TGG + chr1.2:58212128-58212147 None:intergenic 55.0%
AGGACATGCAGTGGGTCCAC+CGG + chr1.2:58211985-58212004 None:intergenic 60.0%
CCACCGGGTAATCACGGTGT+AGG + chr1.2:58211969-58211988 None:intergenic 60.0%
CCGATACGGACGAGACGAAG+CGG + chr1.2:58212176-58212195 None:intergenic 60.0%
CCGCCAGAATCGCACTTTCC+AGG - chr1.2:58212122-58212141 MS.gene066022:intron 60.0%
CCGCTTCGTCTCGTCCGTAT+CGG - chr1.2:58212173-58212192 MS.gene066022:CDS 60.0%
CCTACACCGTGATTACCCGG+TGG - chr1.2:58211966-58211985 MS.gene066022:intron 60.0%
CTCACCGGAGGACATGCAGT+GGG + chr1.2:58211993-58212012 None:intergenic 60.0%
GCACTTCCCACCTCCGTTCA+GGG - chr1.2:58212302-58212321 MS.gene066022:intron 60.0%
GGTCAGTTATTCCGGGACGG+AGG + chr1.2:58209905-58209924 None:intergenic 60.0%
GTCACGCGCGGTGGAAAAAG+CGG + chr1.2:58212281-58212300 None:intergenic 60.0%
GTGGCTGTTCTGGCCGGAAA+AGG + chr1.2:58212097-58212116 None:intergenic 60.0%
GTGGGTCCACCGGGTAATCA+CGG + chr1.2:58211975-58211994 None:intergenic 60.0%
GTGTACTACTCCCTCCGTCC+CGG - chr1.2:58209891-58209910 MS.gene066022:intron 60.0%
TACGGACGAGACGAAGCGGA+TGG + chr1.2:58212172-58212191 None:intergenic 60.0%
TCTCACCGGAGGACATGCAG+TGG + chr1.2:58211994-58212013 None:intergenic 60.0%
TGGACCCACTGCATGTCCTC+CGG - chr1.2:58211986-58212005 MS.gene066022:intron 60.0%
! GGAAAGTGCGATTCTGGCGG+CGG + chr1.2:58212122-58212141 None:intergenic 60.0%
!! GCTTTTTCCACCGCGCGTGA+CGG - chr1.2:58212280-58212299 MS.gene066022:intron 60.0%
CGGTGGAAAAAGCGGCGCGT+GGG + chr1.2:58212273-58212292 None:intergenic 65.0%
GAGAGCAGCGGCACCCTGAA+CGG + chr1.2:58212318-58212337 None:intergenic 65.0%
GCGCGTTGCGACGTTTCGGA+TGG + chr1.2:58212217-58212236 None:intergenic 65.0%
GGACATGCAGTGGGTCCACC+GGG + chr1.2:58211984-58212003 None:intergenic 65.0%
GGACGAGACGAAGCGGATGG+TGG + chr1.2:58212169-58212188 None:intergenic 65.0%
GGCACTTCCCACCTCCGTTC+AGG - chr1.2:58212301-58212320 MS.gene066022:intron 65.0%
TCCGAGTCTGGCGAGAGCAG+CGG + chr1.2:58212330-58212349 None:intergenic 65.0%
! CGTCCGTATCGGCCTGCTTC+CGG - chr1.2:58212184-58212203 MS.gene066022:CDS 65.0%
AGCAGCGGCACCCTGAACGG+AGG + chr1.2:58212315-58212334 None:intergenic 70.0%
AGCGGCACCCTGAACGGAGG+TGG + chr1.2:58212312-58212331 None:intergenic 70.0%
GCCGCTGCTCTCGCCAGACT+CGG - chr1.2:58212326-58212345 MS.gene066022:intron 70.0%
GCGGCACCCTGAACGGAGGT+GGG + chr1.2:58212311-58212330 None:intergenic 70.0%
GCGGCAGTGGCTGTTCTGGC+CGG + chr1.2:58212103-58212122 None:intergenic 70.0%
GCGGGCGCGTTGCGACGTTT+CGG + chr1.2:58212221-58212240 None:intergenic 70.0%
GCGGTGGAAAAAGCGGCGCG+TGG + chr1.2:58212274-58212293 None:intergenic 70.0%
TGCGATTCTGGCGGCGGCAG+TGG + chr1.2:58212116-58212135 None:intergenic 70.0%
!! GTGGGAAGTGCCGTCACGCG+CGG + chr1.2:58212293-58212312 None:intergenic 70.0%
CGGCGCGTGGGAGTTTGCGC+TGG + chr1.2:58212261-58212280 None:intergenic 75.0%
GGCGGCGGCAGTGGCTGTTC+TGG + chr1.2:58212107-58212126 None:intergenic 75.0%
!! GGAAGTGCCGTCACGCGCGG+TGG + chr1.2:58212290-58212309 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 58209144 58212414 58209144 ID=MS.gene066022
chr1.2 mRNA 58209144 58212414 58209144 ID=MS.gene066022.t1;Parent=MS.gene066022
chr1.2 exon 58212343 58212414 58212343 ID=MS.gene066022.t1.exon1;Parent=MS.gene066022.t1
chr1.2 CDS 58212343 58212414 58212343 ID=cds.MS.gene066022.t1;Parent=MS.gene066022.t1
chr1.2 exon 58212152 58212250 58212152 ID=MS.gene066022.t1.exon2;Parent=MS.gene066022.t1
chr1.2 CDS 58212152 58212250 58212152 ID=cds.MS.gene066022.t1;Parent=MS.gene066022.t1
chr1.2 exon 58211694 58211795 58211694 ID=MS.gene066022.t1.exon3;Parent=MS.gene066022.t1
chr1.2 CDS 58211694 58211795 58211694 ID=cds.MS.gene066022.t1;Parent=MS.gene066022.t1
chr1.2 exon 58211234 58211309 58211234 ID=MS.gene066022.t1.exon4;Parent=MS.gene066022.t1
chr1.2 CDS 58211234 58211309 58211234 ID=cds.MS.gene066022.t1;Parent=MS.gene066022.t1
chr1.2 exon 58210069 58210192 58210069 ID=MS.gene066022.t1.exon5;Parent=MS.gene066022.t1
chr1.2 CDS 58210069 58210192 58210069 ID=cds.MS.gene066022.t1;Parent=MS.gene066022.t1
chr1.2 exon 58209938 58209975 58209938 ID=MS.gene066022.t1.exon6;Parent=MS.gene066022.t1
chr1.2 CDS 58209938 58209975 58209938 ID=cds.MS.gene066022.t1;Parent=MS.gene066022.t1
chr1.2 exon 58209144 58209610 58209144 ID=MS.gene066022.t1.exon7;Parent=MS.gene066022.t1
chr1.2 CDS 58209144 58209610 58209144 ID=cds.MS.gene066022.t1;Parent=MS.gene066022.t1
Gene Sequence

>MS.gene066022

ATGTCTGACAAAGTCAACGATGCTTGGAACAAATATCTCCTTCAGCTTAAGCTTCATCCTCTCAGAACCAAGGTTTCATTTTACTATTTTTCTCATTTTCATTTCTAAGAGACTTTTATATTGTTGTTGAAAAATACAAACTTGTGTGTGTTTTGTTATGTTAGGCTATTACTTCATCAGTTCTAGCTGGTTTTAGTGATGCTGTTGCACAGAAGCTTTCTGGTGCTAAGAAGCTTCAGTTCAGAAGGATTCTTCTTTTCATGGTATATTACTTAATTGTTTCTTCTTTACAATTTCTTTACTTTCAATCTATGAAACACTGACACATATGCGAACATCAGACACAACAATGGTACTGACACGTCGATGATACCAGTAATAATTTTAAATGTTATCACATGTGTGTTACCATTGTCGTGTCGAGCACCAAACATGCCTCCAATCAAAAGTATCGGTGCTATAAAACTTTCAATCAGTGATCAATTTTTTAGAAGACAAAAAAATAATCTAAAAACATGTTTATTGCTTACTATGTTATAGATGAAGTATAAGTTATTATAGTTTGATAGTAGTTGAATTTGGATGGGCTGTGGAAATTTAAAATCAAATTATTTTGCAGCTCTATGGTTTCGCCTATGCAGGACCCTTTGGACATTATCTCCATTTATTGATGGATAAACTTTTCAAGGGGAAGAAAGGCAATGAAACTGTTGCTAAGAAGGTATATAGTTAAACTTGTTTTATTGTGTGTACTACTCCCTCCGTCCCGGAATAACTGACCCATTTTCTAAAAAAAAGTGTCCCAAAAATACTGACTCATTTCAATTTTCAATACAACTTTCATCATTCCCAATACAATATAACACTAAATAATACTCTTTTTATCATTCCCAATACAATATATTCCACTTTCCCCTTTATTTATACACTTTTCTTAATCTGTGTGAAAAACCAAAGTAGGTCAATTATACCGGGACGGAGGTAGTATGTCCTTTGGTTATAGGGATAAATAGTATTTTGTGTTGTGTGATCAGGTTCTATGTTTTCCTTAGCCCTGGTACTTGTTTTATGAAAAACACTAATGAATGAAATATCATATTACTAGGTGATCCTTGAGCAAATAACTAGTTCTCCATGGAACAACTTTTTGTTCATGATGTATTATGGCTTGATTATTGAAGGTATGATAATTCTCATTGTTAAAATTCTTATCATATATGTATGCTAGACATTAGTTTAGACAGTGATTTTGAAACATGTACATGAATAAAGATTAATTTCAGATTTTGTTGTAACTTGTAAAGTTGTAATTGAGTGCCTCTTGTGTTGTGTAACCCACCTCTCTACCAAGAAAACAGAATTGACATTCCTCTAATATTTAGTATTAATTGTTTCTTCTTTCTGCTTAATTCCTAACTTTGGTTCTGGTACTATTATGTTGCATTTGAAGTATGTATATGTAATCCGAAGTTCAAAATTATCAATGTTTATAGGTCAATTTGTAACCCTAAGTTTACAATAATCATTATTTACAAGTCAATATGGTGTCTTGATCAACAATTTGTTGCATTAGAAAATCTAGACCTTCTATCCCAAATTTCGGTGCAAAACTTTTCATTTTTATCAAACATAGCCTTAATATGATATTGCGAACAACAAATTAGAAAACATTTATAGAATAAAAGGTCTAACGATTCATGTATCAATCTTACCTTACAAGTATATGAAGGGTCTAACAGCCAAGTACTTACTTTCTTAAATTGTTCTGTTCAATTTAAACTTAACATTGAATTTAATTCCCGGAGGAGAGCAATATAAGGAACTATTTATGCTTGCTTGTGTTACAATCTAGTAGTTTTCATTCGTAAGAAAAACATAAAACTCTAGAAATCTTCACTAAGATAACTTGAACCAAACTGAAATAGTGAAGGGTCTAGAAAAGATAAAACGCATAAACAAATTGTGTGTGTAAGTTTCTTACGTCTCTTGTATAGTTTAATCAAAGAGATACATTGAATGATAGATGAAGTTTAAGCTTTTTGGAAAGCATGCTTCATATTTCGCTGATTTTTACTCACAGAATAGCCTCATGAAACAATTCCAACCTATTGATAATATCTTAATCATGTCACATAATAAAAGCATTTTTCAAATGAAATATACTAAATCAATCTCCTTCACTGCATTGTGCTTTGTAATTAAAGTGTGAGGTTGGTTTGCAGGAAGACCTTGGAATATAGTCATGAACAAAGTCAAAAATGACTACCCTACTGTTCAGTTGACGGCGTGGAAGGTATGGCAATGTCAACAGTCTTATGCTGTTTGGCCAATGGAACAATTTGAATAGTTAGTTTTTGGAAGGCGTAACTAATTAGTTTTTTGAACATGCAGTTTTGGCCTATCGTTGGTTGGGTGAATTACCAGTATATGCCTTTGCAGTTCCGTGTTTTATTTCACAGCTTTGTTGGTTCATGCTGGTGAGTTCTTGTATCTTTCAACTTTTTATTAATTATAATTCTTCAATGATGTTTTCATTTCATCTCTTAATTGTTCCAAAATAATGATTAAGCATTGTTGATATATAAATATATAGTAAATAATTATATTTATGCTCTAAATATATTTTAGCTTACTCTCTAAGTTACTTAGGATATAATATTATGATGAATATTCATTCTGTAAATATATCATAGCTTAGGCCCCAAGCTACTTACTTCTATATAATAAATCTTCTGTTGCGCTATTCAGACACACGGTATTTCATCATATGTCTCTCTTTTCTCTCTTATTCAACATGGTATCAGAGCCTATTGAGAGACAACCCTACACCGTGATTACCCGGTGGACCCACTGCATGTCCTCCGGTGAGAATTTTTTTCGGCAAACCGTGTTTCATCTCCGGTTTGCCCCCTCCTTTATTGCTGTAACAAATTCTGGTGCTCGTTTGAACCCTTTTCCGGCCAGAACAGCCACTGCCGCCGCCAGAATCGCACTTTCCAGGCACTATTCCGGCAAGATAACACCACCATCCGCTTCGTCTCGTCCGTATCGGCCTGCTTCCGGAAAATCACCATCCGAAACGTCGCAACGCGCCCGCACGCGTCTCTTTTCTCTCCAGCGCAAACTCCCACGCGCCGCTTTTTCCACCGCGCGTGACGGCACTTCCCACCTCCGTTCAGGGTGCCGCTGCTCTCGCCAGACTCGGAATTCTTTGCTGATTCCAGATCTGGTCTTTGTTTTTAGTTTTGATCCACGGAAATACGTGCTCTAG
Protein sequence

>MS.gene066022.t1

MSDKVNDAWNKYLLQLKLHPLRTKAITSSVLAGFSDAVAQKLSGAKKLQFRRILLFMLYGFAYAGPFGHYLHLLMDKLFKGKKGNETVAKKVILEQITSSPWNNFLFMMYYGLIIEGRPWNIVMNKVKNDYPTVQLTAWKVWQCQQSYAVWPMEQFEYSVFYFTALLVHAEPIERQPYTVITRWTHCMSSGENFFRQTVFHLRFAPSFIAVTNSGARLNPFPARTATAAARIALSRHYSGKITPPSASSRPYRPASGKSPSETSQRARTRLFSLQRKLPRAAFSTARDGTSHLRSGCRCSRQTRNSLLIPDLVFVFSFDPRKYVL