Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066083.t1 | XP_013470155.1 | 100 | 191 | 0 | 0 | 1 | 191 | 664 | 854 | 3.40E-102 | 380.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066083.t1 | O23617 | 68.5 | 184 | 56 | 1 | 6 | 187 | 669 | 852 | 3.2e-69 | 262.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066083.t1 | A0A072VRW8 | 100.0 | 191 | 0 | 0 | 1 | 191 | 664 | 854 | 2.4e-102 | 380.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052355 | MS.gene066083 | 0.81106 | 8.45E-51 | -1.69E-46 |
| MS.gene058712 | MS.gene066083 | 0.811702 | 6.13E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGTCTTCCAAGTAATATT+TGG | 0.281526 | 1.3:+74632716 | None:intergenic |
| GAATTTGCAGACCGAGATTT+CGG | 0.306462 | 1.3:-74633584 | MS.gene066083:CDS |
| TTGCTTAATGAGGAGATAAC+TGG | 0.325875 | 1.3:+74632625 | None:intergenic |
| CCAAACCAGAGCACTCTCTT+TGG | 0.342936 | 1.3:+74633613 | None:intergenic |
| GGTTTAACTTCCACAATGTA+TGG | 0.356426 | 1.3:+74633485 | None:intergenic |
| TCTGTATATAACTGCATAAC+TGG | 0.363196 | 1.3:+74633662 | None:intergenic |
| AAGCTTCCCATTCTTCAGTA+TGG | 0.363738 | 1.3:+74633726 | None:intergenic |
| AGATCAGATGAGGACATGTT+TGG | 0.364020 | 1.3:-74632819 | MS.gene066083:CDS |
| CCAATGCATAGAACAAAATC+TGG | 0.378049 | 1.3:+74632849 | None:intergenic |
| TTTGTTGCAGGGTGTGAGTA+AGG | 0.391370 | 1.3:-74632922 | MS.gene066083:intron |
| ATCTGTTTCCAGTCAAAATC+AGG | 0.395840 | 1.3:+74633692 | None:intergenic |
| TAAGGAACTTCTTGATCATC+TGG | 0.414096 | 1.3:-74633549 | MS.gene066083:CDS |
| GATCAGATGAGGACATGTTT+GGG | 0.414515 | 1.3:-74632818 | MS.gene066083:CDS |
| TCTGATAACAATGCAACAAA+AGG | 0.423959 | 1.3:-74632880 | MS.gene066083:CDS |
| CCAAAGAGAGTGCTCTGGTT+TGG | 0.428765 | 1.3:-74633613 | MS.gene066083:CDS |
| AAGTAATATTTGGCTTTGCT+TGG | 0.430940 | 1.3:+74632726 | None:intergenic |
| AGCAGTTTGTTCAGAAGCAT+TGG | 0.467175 | 1.3:+74632662 | None:intergenic |
| GACAAGCCATACTGAAGAAT+GGG | 0.468184 | 1.3:-74633732 | MS.gene066083:CDS |
| CAAGGAAACACGTCCGCAAT+GGG | 0.469994 | 1.3:+74632765 | None:intergenic |
| TTAGAAAAGCTTGCTTAATG+AGG | 0.484009 | 1.3:+74632615 | None:intergenic |
| AAGCAAAGCCAAATATTACT+TGG | 0.502945 | 1.3:-74632724 | MS.gene066083:CDS |
| GACGTGTTTCCTTGCACCGT+TGG | 0.505796 | 1.3:-74632756 | MS.gene066083:CDS |
| GGACAAGCCATACTGAAGAA+TGG | 0.510330 | 1.3:-74633733 | MS.gene066083:CDS |
| CTGATAACAATGCAACAAAA+GGG | 0.514427 | 1.3:-74632879 | MS.gene066083:CDS |
| TTGTTGCAGGGTGTGAGTAA+GGG | 0.516408 | 1.3:-74632921 | MS.gene066083:intron |
| CTTGACATGAACCGAAATCT+CGG | 0.524650 | 1.3:+74633573 | None:intergenic |
| CATTGTGGAAGTTAAACCTC+AGG | 0.529575 | 1.3:-74633480 | MS.gene066083:intron |
| GAGCCTGTTTCAGTTAAAAG+TGG | 0.534096 | 1.3:-74633509 | MS.gene066083:CDS |
| GCAAGGAAACACGTCCGCAA+TGG | 0.535831 | 1.3:+74632764 | None:intergenic |
| TTTCGGTTCATGTCAAGCTA+AGG | 0.551647 | 1.3:-74633567 | MS.gene066083:CDS |
| GTTTAACTTCCACAATGTAT+GGG | 0.566536 | 1.3:+74633486 | None:intergenic |
| TGCTTAATGAGGAGATAACT+GGG | 0.586765 | 1.3:+74632626 | None:intergenic |
| AGAGTCCAAAGAGAGTGCTC+TGG | 0.593330 | 1.3:-74633618 | MS.gene066083:CDS |
| TAAAAGTGGCCCATACATTG+TGG | 0.597276 | 1.3:-74633495 | MS.gene066083:CDS |
| TTGGGGTAATAACAAGTGCA+AGG | 0.603403 | 1.3:-74632800 | MS.gene066083:CDS |
| TGGGGTAATAACAAGTGCAA+GGG | 0.633008 | 1.3:-74632799 | MS.gene066083:CDS |
| TGGTGATGACAGATCAGATG+AGG | 0.641223 | 1.3:-74632829 | MS.gene066083:CDS |
| GGCATCTCTATCGCCCATTG+CGG | 0.645098 | 1.3:-74632778 | MS.gene066083:CDS |
| TTATATACAGAAACTACTGA+TGG | 0.662521 | 1.3:-74633650 | MS.gene066083:CDS |
| ATCAGATGAGGACATGTTTG+GGG | 0.667558 | 1.3:-74632817 | MS.gene066083:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAATGATTAATAATACTA+TGG | - | chr1.3:74633308-74633327 | MS.gene066083:intron | 15.0% |
| !! | GATCTTTAGTTAAAATAAAA+AGG | - | chr1.3:74633007-74633026 | MS.gene066083:intron | 15.0% |
| !! | ATTAAGAAACAGACATTTAT+CGG | + | chr1.3:74633073-74633092 | None:intergenic | 20.0% |
| !! | TTGAAGTTAATTCTAATCTT+TGG | - | chr1.3:74633335-74633354 | MS.gene066083:intron | 20.0% |
| !! | TTTGTATACTACTTATTGTT+GGG | - | chr1.3:74632977-74632996 | MS.gene066083:intron | 20.0% |
| ! | AGTTCATGATCTTAGAAATA+AGG | + | chr1.3:74633384-74633403 | None:intergenic | 25.0% |
| ! | ATAAAAAGGATACTTGAATC+TGG | - | chr1.3:74633021-74633040 | MS.gene066083:intron | 25.0% |
| ! | GTTTGTATACTACTTATTGT+TGG | - | chr1.3:74632976-74632995 | MS.gene066083:intron | 25.0% |
| ! | TTATATACAGAAACTACTGA+TGG | - | chr1.3:74632711-74632730 | MS.gene066083:CDS | 25.0% |
| !!! | ACTTTTTTATTACTAACCTG+AGG | + | chr1.3:74632900-74632919 | None:intergenic | 25.0% |
| AAGCAAAGCCAAATATTACT+TGG | - | chr1.3:74633637-74633656 | MS.gene066083:CDS | 30.0% | |
| CTGATAACAATGCAACAAAA+GGG | - | chr1.3:74633482-74633501 | MS.gene066083:CDS | 30.0% | |
| TCATGATCTTAGAAATAAGG+AGG | + | chr1.3:74633381-74633400 | None:intergenic | 30.0% | |
| TCGTAGATCAAAGAAAATAG+CGG | + | chr1.3:74633258-74633277 | None:intergenic | 30.0% | |
| TCTGATAACAATGCAACAAA+AGG | - | chr1.3:74633481-74633500 | MS.gene066083:CDS | 30.0% | |
| TCTGTATATAACTGCATAAC+TGG | + | chr1.3:74632702-74632721 | None:intergenic | 30.0% | |
| TGTGTCTTCCAAGTAATATT+TGG | + | chr1.3:74633648-74633667 | None:intergenic | 30.0% | |
| ! | GTTTAACTTCCACAATGTAT+GGG | + | chr1.3:74632878-74632897 | None:intergenic | 30.0% |
| ! | TGGTCATGAATTCTTTTGAA+GGG | - | chr1.3:74633355-74633374 | MS.gene066083:intron | 30.0% |
| ! | TTGGTCATGAATTCTTTTGA+AGG | - | chr1.3:74633354-74633373 | MS.gene066083:intron | 30.0% |
| !! | AAGTAATATTTGGCTTTGCT+TGG | + | chr1.3:74633638-74633657 | None:intergenic | 30.0% |
| ATCTGTTTCCAGTCAAAATC+AGG | + | chr1.3:74632672-74632691 | None:intergenic | 35.0% | |
| CCAATGCATAGAACAAAATC+TGG | + | chr1.3:74633515-74633534 | None:intergenic | 35.0% | |
| TAAGGAACTTCTTGATCATC+TGG | - | chr1.3:74632812-74632831 | MS.gene066083:CDS | 35.0% | |
| ! | CACTTGACATTTTTGTTGCA+GGG | - | chr1.3:74633428-74633447 | MS.gene066083:intron | 35.0% |
| ! | CCAGATTTTGTTCTATGCAT+TGG | - | chr1.3:74633512-74633531 | MS.gene066083:CDS | 35.0% |
| ! | GGTTTAACTTCCACAATGTA+TGG | + | chr1.3:74632879-74632898 | None:intergenic | 35.0% |
| !! | TTTGCTTGGTTTTTGACCAA+CGG | + | chr1.3:74633624-74633643 | None:intergenic | 35.0% |
| AAGCTTCCCATTCTTCAGTA+TGG | + | chr1.3:74632638-74632657 | None:intergenic | 40.0% | |
| AGATCAGATGAGGACATGTT+TGG | - | chr1.3:74633542-74633561 | MS.gene066083:CDS | 40.0% | |
| ATCAGATGAGGACATGTTTG+GGG | - | chr1.3:74633544-74633563 | MS.gene066083:CDS | 40.0% | |
| CATTGTGGAAGTTAAACCTC+AGG | - | chr1.3:74632881-74632900 | MS.gene066083:CDS | 40.0% | |
| CCTGCAACAAAAATGTCAAG+TGG | + | chr1.3:74633430-74633449 | None:intergenic | 40.0% | |
| CTTGACATGAACCGAAATCT+CGG | + | chr1.3:74632791-74632810 | None:intergenic | 40.0% | |
| GAATTTGCAGACCGAGATTT+CGG | - | chr1.3:74632777-74632796 | MS.gene066083:CDS | 40.0% | |
| GACAAGCCATACTGAAGAAT+GGG | - | chr1.3:74632629-74632648 | MS.gene066083:CDS | 40.0% | |
| GAGCCTGTTTCAGTTAAAAG+TGG | - | chr1.3:74632852-74632871 | MS.gene066083:CDS | 40.0% | |
| GATCAGATGAGGACATGTTT+GGG | - | chr1.3:74633543-74633562 | MS.gene066083:CDS | 40.0% | |
| TAAAAGTGGCCCATACATTG+TGG | - | chr1.3:74632866-74632885 | MS.gene066083:CDS | 40.0% | |
| TGATCTACGATTGACAACAC+TGG | - | chr1.3:74633268-74633287 | MS.gene066083:intron | 40.0% | |
| TGGGGTAATAACAAGTGCAA+GGG | - | chr1.3:74633562-74633581 | MS.gene066083:CDS | 40.0% | |
| TTGGGGTAATAACAAGTGCA+AGG | - | chr1.3:74633561-74633580 | MS.gene066083:CDS | 40.0% | |
| TTTCGGTTCATGTCAAGCTA+AGG | - | chr1.3:74632794-74632813 | MS.gene066083:CDS | 40.0% | |
| ! | AAGACACAAGCGAGATTTTG+AGG | - | chr1.3:74633660-74633679 | MS.gene066083:CDS | 40.0% |
| ! | AGCAGTTTGTTCAGAAGCAT+TGG | + | chr1.3:74633702-74633721 | None:intergenic | 40.0% |
| ! | CCACTTGACATTTTTGTTGC+AGG | - | chr1.3:74633427-74633446 | MS.gene066083:intron | 40.0% |
| !! | GAGATTTTGAGGATGTTGCA+AGG | - | chr1.3:74633671-74633690 | MS.gene066083:CDS | 40.0% |
| !! | TTTCTGTGCCTGATTTTGAC+TGG | - | chr1.3:74632661-74632680 | MS.gene066083:CDS | 40.0% |
| AGAACCATGTTACGCTAGCA+TGG | - | chr1.3:74633047-74633066 | MS.gene066083:intron | 45.0% | |
| ATAGTAGCGCTACAACGTAG+TGG | - | chr1.3:74633119-74633138 | MS.gene066083:intron | 45.0% | |
| TGCTAGCGTAACATGGTTCT+AGG | + | chr1.3:74633047-74633066 | None:intergenic | 45.0% | |
| TTGTTGCAGGGTGTGAGTAA+GGG | - | chr1.3:74633440-74633459 | MS.gene066083:intron | 45.0% | |
| TTTGTTGCAGGGTGTGAGTA+AGG | - | chr1.3:74633439-74633458 | MS.gene066083:intron | 45.0% | |
| ! | TGGTGATGACAGATCAGATG+AGG | - | chr1.3:74633532-74633551 | MS.gene066083:CDS | 45.0% |
| !! | CAACACTGGCTTGAAGTAGT+TGG | - | chr1.3:74633282-74633301 | MS.gene066083:intron | 45.0% |
| !!! | GGGCCACTTTTAACTGAAAC+AGG | + | chr1.3:74632858-74632877 | None:intergenic | 45.0% |
| CAAGGAAACACGTCCGCAAT+GGG | + | chr1.3:74633599-74633618 | None:intergenic | 50.0% | |
| ! | CCAAACCAGAGCACTCTCTT+TGG | + | chr1.3:74632751-74632770 | None:intergenic | 50.0% |
| ! | GGTTTTTGACCAACGGTGCA+AGG | + | chr1.3:74633617-74633636 | None:intergenic | 50.0% |
| !! | AGAGTCCAAAGAGAGTGCTC+TGG | - | chr1.3:74632743-74632762 | MS.gene066083:CDS | 50.0% |
| !! | CCAAAGAGAGTGCTCTGGTT+TGG | - | chr1.3:74632748-74632767 | MS.gene066083:CDS | 50.0% |
| GACGTGTTTCCTTGCACCGT+TGG | - | chr1.3:74633605-74633624 | MS.gene066083:CDS | 55.0% | |
| GCAAGGAAACACGTCCGCAA+TGG | + | chr1.3:74633600-74633619 | None:intergenic | 55.0% | |
| GGCATCTCTATCGCCCATTG+CGG | - | chr1.3:74633583-74633602 | MS.gene066083:CDS | 55.0% | |
| TCGGCCATGCTAGCGTAACA+TGG | + | chr1.3:74633054-74633073 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 74632629 | 74633754 | 74632629 | ID=MS.gene066083 |
| chr1.3 | mRNA | 74632629 | 74633754 | 74632629 | ID=MS.gene066083.t1;Parent=MS.gene066083 |
| chr1.3 | exon | 74633481 | 74633754 | 74633481 | ID=MS.gene066083.t1.exon1;Parent=MS.gene066083.t1 |
| chr1.3 | CDS | 74633481 | 74633754 | 74633481 | ID=cds.MS.gene066083.t1;Parent=MS.gene066083.t1 |
| chr1.3 | exon | 74632629 | 74632934 | 74632629 | ID=MS.gene066083.t1.exon2;Parent=MS.gene066083.t1 |
| chr1.3 | CDS | 74632629 | 74632934 | 74632629 | ID=cds.MS.gene066083.t1;Parent=MS.gene066083.t1 |
| Gene Sequence |
| Protein sequence |
