AlfalfaGEDB | MS.gene058712

M. sativa cultivar XinJiangDaYe / MS.gene058712

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12799.10 PF13855.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058712.t1	GAU47049.1	53.5	492	109	7	4	495	5	376	2.70E-50	209.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058712.t1	O80809	50.0	202	101	0	294	495	518	719	6.9e-15	83.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058712.t1	A0A2Z6PKZ2	53.5	492	109	7	4	495	5	376	1.9e-50	209.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052611	MS.gene058712	0.807364	5.24E-50	-1.69E-46
MS.gene058712	MS.gene066083	0.811702	6.13E-51	-1.69E-46
MS.gene058712	MS.gene51180	0.882313	1.16E-70	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene00013	MS.gene058712	PPI
MS.gene02153	MS.gene058712	PPI
MS.gene02723	MS.gene058712	PPI
MS.gene029081	MS.gene058712	PPI
MS.gene044334	MS.gene058712	PPI
MS.gene046120	MS.gene058712	PPI
MS.gene047988	MS.gene058712	PPI
MS.gene25695	MS.gene058712	PPI
MS.gene70469	MS.gene058712	PPI
MS.gene71307	MS.gene058712	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058712.t1	MTR_7g079550	76.726	507	96	5	6	496	219	719	0.0	733
MS.gene058712.t1	MTR_7g079550	51.896	422	151	9	1	412	119	498	2.54e-112	348
MS.gene058712.t1	MTR_7g079550	31.548	336	172	10	85	412	93	378	5.76e-21	97.1
MS.gene058712.t1	MTR_7g079550	30.882	272	157	7	163	414	72	332	7.66e-19	90.1
MS.gene058712.t1	MTR_1g101250	36.034	469	257	15	3	431	604	1069	2.16e-54	197
MS.gene058712.t1	MTR_1g101250	29.116	498	279	12	3	446	359	836	3.57e-32	131
MS.gene058712.t1	MTR_1g101250	28.231	503	227	13	3	417	264	720	1.66e-30	126
MS.gene058712.t1	MTR_1g101250	27.470	506	264	15	3	416	153	647	1.35e-24	108
MS.gene058712.t1	MTR_1g101250	26.549	565	306	18	17	480	118	674	2.45e-23	104
MS.gene058712.t1	MTR_1g101250	25.000	484	257	17	43	427	87	563	1.91e-14	76.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058712.t1	AT1G65380	52.372	506	226	5	3	496	218	720	1.66e-160	473
MS.gene058712.t1	AT1G65380	39.430	421	203	7	2	412	122	500	2.44e-72	243
MS.gene058712.t1	AT1G65380	30.655	336	204	8	96	409	121	449	1.10e-25	111
MS.gene058712.t1	AT1G65380	27.358	318	166	7	1	274	315	611	3.86e-21	97.4
MS.gene058712.t1	AT1G65380	32.168	286	148	9	145	414	79	334	1.24e-16	83.6
MS.gene058712.t1	AT5G49290	31.934	548	300	17	2	483	125	665	3.28e-49	180
MS.gene058712.t1	AT5G49290	26.464	427	243	11	52	412	3	424	1.42e-16	83.2
MS.gene058712.t1	AT5G49290	23.981	417	199	12	96	412	2	400	5.53e-14	75.1
MS.gene058712.t1	AT5G49290	31.934	548	300	17	2	483	340	880	1.30e-48	180
MS.gene058712.t1	AT5G49290	30.841	428	241	15	2	415	94	480	1.28e-25	111

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Find 105 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAACTTCCTTGCTTTGA+TGG	0.256134	7.4:-26371685	None:intergenic
AAGAAAATCCCTACAGATAT+TGG	0.330681	7.4:-26371730	None:intergenic
AAGCAAGAGAAAGATACCTG+AGG	0.704950	7.4:-26371057	None:intergenic
AATCTTGAAAGATTACATCT+TGG	0.461585	7.4:+26370191	MS.gene058712:CDS
AATGAGAATCCATCTTTGGG+AGG	0.590436	7.4:+26370134	MS.gene058712:CDS
ACCATTCCATTCAGTATTGT+TGG	0.357668	7.4:+26370809	MS.gene058712:intron
ACCTTTGTAGCTTCAACAAA+AGG	0.437628	7.4:-26371235	None:intergenic
ACTTATCAAGGAATCATTTG+AGG	0.507955	7.4:+26370486	MS.gene058712:CDS
AGACAAATTCAACACAGTTA+AGG	0.426793	7.4:-26370399	None:intergenic
AGATGTATCAAATCAAGTTT+TGG	0.352820	7.4:-26371833	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATAACTTCCTTGCTTTGA+TGG	-	chr7.4:26371688-26371707	None:intergenic	30.0%
AAGAAAATCCCTACAGATAT+TGG	-	chr7.4:26371733-26371752	None:intergenic	30.0%
ACTTATCAAGGAATCATTTG+AGG	+	chr7.4:26370486-26370505	MS.gene058712:CDS	30.0%
AGACAAATTCAACACAGTTA+AGG	-	chr7.4:26370402-26370421	None:intergenic	30.0%
CTACCCTTCAAATTAATATC+AGG	-	chr7.4:26371178-26371197	None:intergenic	30.0%
CTTAGTCATAATAGGTTTCA+TGG	+	chr7.4:26370059-26370078	MS.gene058712:CDS	30.0%
GACTAAGATCAATAACTCTA+AGG	-	chr7.4:26370046-26370065	None:intergenic	30.0%
TATGATCTTTCATCGATGTT+CGG	+	chr7.4:26371313-26371332	MS.gene058712:CDS	30.0%
TGAAAATTAGCAATACAAGC+AGG	-	chr7.4:26370449-26370468	None:intergenic	30.0%
TGACTCATTTGAACTTATCA+AGG	+	chr7.4:26370474-26370493	MS.gene058712:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	26370038	26371843	26370038	ID=MS.gene058712
chr7.4	mRNA	26370038	26371843	26370038	ID=MS.gene058712.t1;Parent=MS.gene058712
chr7.4	exon	26370038	26370507	26370038	ID=MS.gene058712.t1.exon1;Parent=MS.gene058712.t1
chr7.4	CDS	26370038	26370507	26370038	ID=cds.MS.gene058712.t1;Parent=MS.gene058712.t1
chr7.4	exon	26370823	26371843	26370823	ID=MS.gene058712.t1.exon2;Parent=MS.gene058712.t1
chr7.4	CDS	26370823	26371843	26370823	ID=cds.MS.gene058712.t1;Parent=MS.gene058712.t1

Gene Sequence

>MS.gene058712

ATGAACCTTAGAGTTATTGATCTTAGTCATAATAGGTTTCATGGTGGAATCCCAAACTCTTTCATGAGGCTTAAGCATCTCACTGAGCTTGTTTTGAATGAGAATCCATCTTTGGGAGGTTTATTGCCTTTTTGGTTAGGTAACTTCTCTGTGAATCTTGAAAGATTACATCTTGGTTATTGTTCATTCAGTGGAAGTATACCTGAGAGTTTGCTTTATTTGAAGTCTCTTAAGTATTTGGACCTTGGAAGCAATTTATTGTCTGGTAATCTTGTTGATTTTCAGCAGTCTTTGGTTCTTCTGAACCTTGGTTCCAATCAGTTTACAGGTACTTTGCCTTGTTTTGCAACTTCAGTTCAGTCCTTAACTGTGTTGAATTTGTCTAACAATTCTATTGTGGGGGGTTTACCTGCTTGTATTGCTAATTTTCAAGCTTTGACTCATTTGAACTTATCAAGGAATCATTTGAGGTATAGAATATATTCAAGGCTTGTGTTCTCAGAGAAACTTGTTGTTTTGGATTTGAGTAATAATGAGTTGTCTGGTCCTATTCCTAGTAAAATTGCAGAGACCACAGAGAAACTTGGTCTTGTTTTTCTTGACCTGTCTCACAATCAATTCTCTGGTGAAATTCCGTTGAAAATTACTGAGTTGAAAAGCTTGCAGGCTTTGTTCCTTTCTCACAATCTTCTTTCTGGAGAAATTCCTGCTAGAATTGGAAATTTGACTTATCTTCAAGTCATTGATATTTCACACAACTCTTTGTCTGGTACCATTCCATTCAGTATTGTTGGGTGCTTTCAGTTGTATGCTTTAATACTTAATAATAACAATCTTTCTGGTGTGATTCAACCGGAGTTTGACGCGTTGGATATCTTGAGAATACTAGATATAAGCAACAATAGGTTTTCCGGGGCTATTCCACTTACTTTGGCTGGTTGTAAATCTTTGGAGATTGTAGATTTTAGTTCCAATGATCTTTCTGGATCCTTGAACGATGCAATAACCAAATGGACAAACCTCAGGTATCTTTCTCTTGCTTGGAACAAATTCGATGGAAACTTGCCTAGTTGGTTGTTCGCATTTCAAGCTATTGAAACAATGGATTTGTCACACAACAAGTTTTCTGGCTTTATACCTGATATTAATTTGAAGGGTAGCTTATTATTTAACACAAGGAATATTACTGTTAAAGAGCCTTTTGTTGAAGCTACAAAGGTGTTTGAACCAAGAGTTTCAGTAGCTGTTTCTGATAGCAATCAACTCAGTTTCACTTATGATCTTTCATCGATGTTCGGAATCGATCTATCCAATAACTTGTTGCATGGTGAGATTCCAAGGGGCTTATTTGGCTTAGCTGGCTTAGAATATCTGAATTTGTCAAACAATTTTCTCAATGGACAGCTTCCTGGTTTGCAGAAAATGCAGAGTTTGAAAGCTATAGATTTGTCGCATAATTCCCTATCAGGACATATCCCGGGAAACATTTCCAGCCTTCAAGATCTTACCATCTTGAATCTGTCTTACAACTGTTTCTCTGGATATGTTCCTCAGAAGCAAGGTTATGGAAGATTTCCTGGTGCATTTGCTGGAAATCCAGATTTATGCTTGGAATCTCCCAGTGGAGTGTGTGAGGATGGGAGAATTCCATCAAAGCAAGGAAGTTATTTTAGGGAAGATAAGATGGATGGACCAATATCTGTAGGGATTTTCTTTATCAGTGCTTTTGTTAGTTTTGATTTTGGTGTTGTTGTTCTATTCTGTTCTGCTCGAACAAGAAAGTACATTCTCCAAACCAAAACTTGA

Protein sequence

>MS.gene058712.t1

MNLRVIDLSHNRFHGGIPNSFMRLKHLTELVLNENPSLGGLLPFWLGNFSVNLERLHLGYCSFSGSIPESLLYLKSLKYLDLGSNLLSGNLVDFQQSLVLLNLGSNQFTGTLPCFATSVQSLTVLNLSNNSIVGGLPACIANFQALTHLNLSRNHLSIVGCFQLYALILNNNNLSGVIQPEFDALDILRILDISNNRFSGAIPLTLAGCKSLEIVDFSSNDLSGSLNDAITKWTNLRYLSLAWNKFDGNLPSWLFAFQAIETMDLSHNKFSGFIPDINLKGSLLFNTRNITVKEPFVEATKVFEPRVSVAVSDSNQLSFTYDLSSMFGIDLSNNLLHGEIPRGLFGLAGLEYLNLSNNFLNGQLPGLQKMQSLKAIDLSHNSLSGHIPGNISSLQDLTILNLSYNCFSGYVPQKQGYGRFPGAFAGNPDLCLESPSGVCEDGRIPSKQGSYFREDKMDGPISVGIFFISAFVSFDFGVVVLFCSARTRKYILQTKT