Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052355.t1 | XP_013456605.1 | 100 | 111 | 0 | 0 | 7 | 117 | 101 | 211 | 2.90E-56 | 227.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052355.t1 | A0A072UMM0 | 100.0 | 111 | 0 | 0 | 7 | 117 | 101 | 211 | 2.1e-56 | 227.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052355 | MS.gene055358 | 0.804959 | 1.68E-49 | -1.69E-46 |
| MS.gene052355 | MS.gene066083 | 0.81106 | 8.45E-51 | -1.69E-46 |
| MS.gene052355 | MS.gene75304 | 0.82222 | 2.66E-53 | -1.69E-46 |
| MS.gene052355 | MS.gene91807 | 0.805717 | 1.17E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052355.t1 | MTR_4g077250 | 100.000 | 111 | 0 | 0 | 7 | 117 | 101 | 211 | 8.54e-79 | 231 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052355.t1 | AT5G17210 | 60.714 | 112 | 37 | 3 | 7 | 117 | 39 | 144 | 5.10e-42 | 135 |
| MS.gene052355.t1 | AT5G17210 | 60.714 | 112 | 37 | 3 | 7 | 117 | 104 | 209 | 1.77e-41 | 136 |
Find 34 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTTGCAAGTGTTGTATT+TGG | 0.176742 | 4.4:-33405646 | MS.gene052355:CDS |
| TGTCTATTACATCACCTTAA+TGG | 0.223570 | 4.4:-33405620 | MS.gene052355:CDS |
| TTTGACAACATAACAGTAAT+AGG | 0.318480 | 4.4:+33405699 | None:intergenic |
| TCACCTTTCATATTCATTCA+TGG | 0.326961 | 4.4:+33406782 | None:intergenic |
| ATGGCTATGGTCCCTTGATT+AGG | 0.386220 | 4.4:+33405556 | None:intergenic |
| GTCAAACCTGGAGTGTTTGC+TGG | 0.390827 | 4.4:-33405682 | MS.gene052355:CDS |
| CTAGTTTGAGTTGGAATTTG+TGG | 0.407106 | 4.4:+33405526 | None:intergenic |
| AAGAACGCTAGAACTCCTTT+TGG | 0.431048 | 4.4:-33405592 | MS.gene052355:CDS |
| TACTGTTATGTTGTCAAACC+TGG | 0.437460 | 4.4:-33405694 | MS.gene052355:CDS |
| GCATTTCTGCTGTTGCTAAC+CGG | 0.443363 | 4.4:-33405769 | MS.gene052355:CDS |
| AGCGTTCTTTCCCTCCATTA+AGG | 0.457539 | 4.4:+33405606 | None:intergenic |
| GATTCCTCATATCCTAATCA+AGG | 0.463814 | 4.4:-33405568 | MS.gene052355:CDS |
| AACTCAAACTAGTCAAGATC+CGG | 0.487221 | 4.4:-33405515 | MS.gene052355:CDS |
| TTGTGGCTGTCCCATGGCTA+TGG | 0.501751 | 4.4:+33405543 | None:intergenic |
| GGTCCCTTGATTAGGATATG+AGG | 0.503319 | 4.4:+33405564 | None:intergenic |
| GTGTCTTCATGCACAAATAC+CGG | 0.503435 | 4.4:+33405496 | None:intergenic |
| GGATCTTGACTAGTTTGAGT+TGG | 0.505716 | 4.4:+33405517 | None:intergenic |
| TGGAATTTGTGGCTGTCCCA+TGG | 0.520604 | 4.4:+33405537 | None:intergenic |
| GCGCCACCAGCAAACACTCC+AGG | 0.542603 | 4.4:+33405676 | None:intergenic |
| TCCATCATGTGAACTGTCGC+CGG | 0.554011 | 4.4:+33405467 | None:intergenic |
| GGATATGAGGAATCACCAAA+AGG | 0.562291 | 4.4:+33405577 | None:intergenic |
| TGCATGAAGACACCTACATC+CGG | 0.563881 | 4.4:-33405486 | MS.gene052355:CDS |
| CTATTACATCACCTTAATGG+AGG | 0.567068 | 4.4:-33405617 | MS.gene052355:CDS |
| TAACCATGAATGAATATGAA+AGG | 0.572172 | 4.4:-33406785 | None:intergenic |
| TGTGAACTGTCGCCGGATGT+AGG | 0.589100 | 4.4:+33405474 | None:intergenic |
| TCCGGCGACAGTTCACATGA+TGG | 0.590189 | 4.4:-33405468 | None:intergenic |
| TAATCAAGGGACCATAGCCA+TGG | 0.594106 | 4.4:-33405554 | MS.gene052355:CDS |
| GGTGAAGAGAGTGTATACTT+TGG | 0.619338 | 4.4:-33405724 | MS.gene052355:CDS |
| TATTACATCACCTTAATGGA+GGG | 0.620550 | 4.4:-33405616 | MS.gene052355:CDS |
| ATTCCTCATATCCTAATCAA+GGG | 0.620663 | 4.4:-33405567 | MS.gene052355:CDS |
| TTGATCATTCAATGCAGCAC+CGG | 0.632758 | 4.4:+33405750 | None:intergenic |
| AATCAAGGGACCATAGCCAT+GGG | 0.657477 | 4.4:-33405553 | MS.gene052355:CDS |
| AAACCTGGAGTGTTTGCTGG+TGG | 0.681637 | 4.4:-33405679 | MS.gene052355:CDS |
| GCTGCATTGAATGATCAACG+CGG | 0.916633 | 4.4:-33405745 | MS.gene052355:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGTAGAAGATATATAAT+AGG | + | chr4.4:33406413-33406432 | None:intergenic | 15.0% |
| !!! | TGCTATATACTAATATTTTT+TGG | + | chr4.4:33406178-33406197 | None:intergenic | 15.0% |
| !! | ATTAAAAATCTATACTGATG+AGG | - | chr4.4:33405945-33405964 | MS.gene052355:intron | 20.0% |
| !! | ATTATAATCTCTATTCAAAC+AGG | - | chr4.4:33406444-33406463 | MS.gene052355:intron | 20.0% |
| !!! | ACTTTTTTTACTCTATCATT+TGG | - | chr4.4:33406006-33406025 | MS.gene052355:intron | 20.0% |
| !!! | ATTAAGTTGTTTATCCAAAT+AGG | + | chr4.4:33406303-33406322 | None:intergenic | 20.0% |
| !!! | CTTTTTTTACTCTATCATTT+GGG | - | chr4.4:33406007-33406026 | MS.gene052355:intron | 20.0% |
| !!! | TTAAGTTGTTTATCCAAATA+GGG | + | chr4.4:33406302-33406321 | None:intergenic | 20.0% |
| !!! | TTTTTTTACTCTATCATTTG+GGG | - | chr4.4:33406008-33406027 | MS.gene052355:intron | 20.0% |
| ! | AACTTATTAGTAGACATTGA+AGG | - | chr4.4:33405822-33405841 | MS.gene052355:intron | 25.0% |
| ! | CTAAAATGTTAACATGAACA+TGG | + | chr4.4:33405918-33405937 | None:intergenic | 25.0% |
| ! | TTAATTAAGTGATTGTGTGT+TGG | - | chr4.4:33406318-33406337 | MS.gene052355:intron | 25.0% |
| ! | TTTGACAACATAACAGTAAT+AGG | + | chr4.4:33406555-33406574 | None:intergenic | 25.0% |
| !! | CTTAAATGTACATTTGCTTT+TGG | - | chr4.4:33405871-33405890 | MS.gene052355:intron | 25.0% |
| AAGTTACATAGTCATAGCAA+TGG | + | chr4.4:33405507-33405526 | None:intergenic | 30.0% | |
| ATGACTATGTAACTTCTTCA+GGG | - | chr4.4:33405512-33405531 | MS.gene052355:CDS | 30.0% | |
| ATGTTCAAAAAACCAGTGAT+AGG | + | chr4.4:33406047-33406066 | None:intergenic | 30.0% | |
| ATTCCTCATATCCTAATCAA+GGG | - | chr4.4:33406684-33406703 | MS.gene052355:intron | 30.0% | |
| ATTGTCATAAACGTTTCAAC+TGG | - | chr4.4:33405635-33405654 | MS.gene052355:CDS | 30.0% | |
| CATCAGAAGTTTATTCAAAC+TGG | + | chr4.4:33406101-33406120 | None:intergenic | 30.0% | |
| TATGACTATGTAACTTCTTC+AGG | - | chr4.4:33405511-33405530 | MS.gene052355:CDS | 30.0% | |
| TATTACATCACCTTAATGGA+GGG | - | chr4.4:33406635-33406654 | MS.gene052355:intron | 30.0% | |
| TGTCTATTACATCACCTTAA+TGG | - | chr4.4:33406631-33406650 | MS.gene052355:intron | 30.0% | |
| TTACCATGATAAAACAAAGC+AGG | + | chr4.4:33405720-33405739 | None:intergenic | 30.0% | |
| TTGTCATAAACGTTTCAACT+GGG | - | chr4.4:33405636-33405655 | MS.gene052355:CDS | 30.0% | |
| !! | TTGTTTGTTATAGTCCTGTT+TGG | - | chr4.4:33405757-33405776 | MS.gene052355:CDS | 30.0% |
| AAACACTTGTATGTTCACAC+GGG | - | chr4.4:33406240-33406259 | MS.gene052355:intron | 35.0% | |
| AACTCAAACTAGTCAAGATC+CGG | - | chr4.4:33406736-33406755 | MS.gene052355:intron | 35.0% | |
| AACTTCTGATGAAACACTCA+CGG | - | chr4.4:33406110-33406129 | MS.gene052355:intron | 35.0% | |
| ACTAACATCAAAGTCAGACT+AGG | - | chr4.4:33406372-33406391 | MS.gene052355:intron | 35.0% | |
| CAAACACTTGTATGTTCACA+CGG | - | chr4.4:33406239-33406258 | MS.gene052355:intron | 35.0% | |
| CTATTACATCACCTTAATGG+AGG | - | chr4.4:33406634-33406653 | MS.gene052355:intron | 35.0% | |
| GAGGTTTACATGAATATGCA+TGG | - | chr4.4:33405964-33405983 | MS.gene052355:intron | 35.0% | |
| GATTCCTCATATCCTAATCA+AGG | - | chr4.4:33406683-33406702 | MS.gene052355:intron | 35.0% | |
| TACTGTTATGTTGTCAAACC+TGG | - | chr4.4:33406557-33406576 | MS.gene052355:intron | 35.0% | |
| TGTGAACATACAAGTGTTTG+TGG | + | chr4.4:33406239-33406258 | None:intergenic | 35.0% | |
| ! | CAACTTGTTTATCCTATCAC+TGG | - | chr4.4:33406032-33406051 | MS.gene052355:intron | 35.0% |
| ! | GGAAAATTTAGAATCGTTGC+TGG | + | chr4.4:33406157-33406176 | None:intergenic | 35.0% |
| ! | TCTCTTGCAAGTGTTGTATT+TGG | - | chr4.4:33406605-33406624 | MS.gene052355:intron | 35.0% |
| !! | CTAGTTTGAGTTGGAATTTG+TGG | + | chr4.4:33406728-33406747 | None:intergenic | 35.0% |
| ATGATAAAACAAAGCAGGCC+AGG | + | chr4.4:33405715-33405734 | None:intergenic | 40.0% | |
| CATTTCCGTTGGTGTAAGTA+TGG | - | chr4.4:33406263-33406282 | MS.gene052355:intron | 40.0% | |
| GGATATGAGGAATCACCAAA+AGG | + | chr4.4:33406677-33406696 | None:intergenic | 40.0% | |
| GTGTCTTCATGCACAAATAC+CGG | + | chr4.4:33406758-33406777 | None:intergenic | 40.0% | |
| GTGTGTTGGATTTCAGAACT+TGG | - | chr4.4:33406332-33406351 | MS.gene052355:intron | 40.0% | |
| GTTTATCCAAATAGGGTCCA+AGG | + | chr4.4:33406295-33406314 | None:intergenic | 40.0% | |
| TGATAAAACAAAGCAGGCCA+GGG | + | chr4.4:33405714-33405733 | None:intergenic | 40.0% | |
| TTGATCATTCAATGCAGCAC+CGG | + | chr4.4:33406504-33406523 | None:intergenic | 40.0% | |
| ! | AAGAACGCTAGAACTCCTTT+TGG | - | chr4.4:33406659-33406678 | MS.gene052355:intron | 40.0% |
| ! | GGTGAAGAGAGTGTATACTT+TGG | - | chr4.4:33406527-33406546 | MS.gene052355:intron | 40.0% |
| !! | GGATCTTGACTAGTTTGAGT+TGG | + | chr4.4:33406737-33406756 | None:intergenic | 40.0% |
| !!! | TGGCCTGCTTTGTTTTATCA+TGG | - | chr4.4:33405714-33405733 | MS.gene052355:CDS | 40.0% |
| AATCAAGGGACCATAGCCAT+GGG | - | chr4.4:33406698-33406717 | MS.gene052355:intron | 45.0% | |
| AGCGTTCTTTCCCTCCATTA+AGG | + | chr4.4:33406648-33406667 | None:intergenic | 45.0% | |
| ATAGTCCTGTTTGGCTATGC+TGG | - | chr4.4:33405766-33405785 | MS.gene052355:CDS | 45.0% | |
| ATGGCTATGGTCCCTTGATT+AGG | + | chr4.4:33406698-33406717 | None:intergenic | 45.0% | |
| CAAGGCCATACTTACACCAA+CGG | + | chr4.4:33406271-33406290 | None:intergenic | 45.0% | |
| CACAGATCCCTGATTCCAAT+TGG | - | chr4.4:33405681-33405700 | MS.gene052355:CDS | 45.0% | |
| GAACATGTGATGTGTGTGTG+TGG | - | chr4.4:33406061-33406080 | MS.gene052355:intron | 45.0% | |
| GATCCCTGATTCCAATTGGA+CGG | - | chr4.4:33405685-33405704 | MS.gene052355:CDS | 45.0% | |
| GCATTTCTGCTGTTGCTAAC+CGG | - | chr4.4:33406482-33406501 | MS.gene052355:intron | 45.0% | |
| GCTGCATTGAATGATCAACG+CGG | - | chr4.4:33406506-33406525 | MS.gene052355:intron | 45.0% | |
| GGTCCCTTGATTAGGATATG+AGG | + | chr4.4:33406690-33406709 | None:intergenic | 45.0% | |
| TAATCAAGGGACCATAGCCA+TGG | - | chr4.4:33406697-33406716 | MS.gene052355:intron | 45.0% | |
| TGCATGAAGACACCTACATC+CGG | - | chr4.4:33406765-33406784 | MS.gene052355:intron | 45.0% | |
| TGGATTTCAGAACTTGGTCG+TGG | - | chr4.4:33406338-33406357 | MS.gene052355:intron | 45.0% | |
| !! | CGAGGATATGTGCACTGATT+TGG | + | chr4.4:33405560-33405579 | None:intergenic | 45.0% |
| AGAGCTGCAGTTAGACCAAG+AGG | + | chr4.4:33405596-33405615 | None:intergenic | 50.0% | |
| CCACCGTCCAATTGGAATCA+GGG | + | chr4.4:33405691-33405710 | None:intergenic | 50.0% | |
| CGTTGGTGTAAGTATGGCCT+TGG | - | chr4.4:33406269-33406288 | MS.gene052355:intron | 50.0% | |
| CTCTACCAGCATAGCCAAAC+AGG | + | chr4.4:33405774-33405793 | None:intergenic | 50.0% | |
| GTTAGACCAAGAGGTAGAGC+TGG | + | chr4.4:33405587-33405606 | None:intergenic | 50.0% | |
| TGGAATTTGTGGCTGTCCCA+TGG | + | chr4.4:33406717-33406736 | None:intergenic | 50.0% | |
| ! | AAACCTGGAGTGTTTGCTGG+TGG | - | chr4.4:33406572-33406591 | MS.gene052355:intron | 50.0% |
| ! | GTCAAACCTGGAGTGTTTGC+TGG | - | chr4.4:33406569-33406588 | MS.gene052355:intron | 50.0% |
| ! | TGTAAGTATGGCCTTGGCCT+TGG | - | chr4.4:33406275-33406294 | MS.gene052355:intron | 50.0% |
| AGAGGTAGAGCTGGACTTCG+AGG | + | chr4.4:33405578-33405597 | None:intergenic | 55.0% | |
| CCAAATAGGGTCCAAGGCCA+AGG | + | chr4.4:33406289-33406308 | None:intergenic | 55.0% | |
| CCCTGATTCCAATTGGACGG+TGG | - | chr4.4:33405688-33405707 | MS.gene052355:CDS | 55.0% | |
| CGAAGTCCAGCTCTACCTCT+TGG | - | chr4.4:33405578-33405597 | MS.gene052355:CDS | 55.0% | |
| GCCACCGTCCAATTGGAATC+AGG | + | chr4.4:33405692-33405711 | None:intergenic | 55.0% | |
| GTTCACACGGGCATTTCCGT+TGG | - | chr4.4:33406252-33406271 | MS.gene052355:intron | 55.0% | |
| TGTGAACTGTCGCCGGATGT+AGG | + | chr4.4:33406780-33406799 | None:intergenic | 55.0% | |
| TTGTGGCTGTCCCATGGCTA+TGG | + | chr4.4:33406711-33406730 | None:intergenic | 55.0% | |
| !! | CCTTGGCCTTGGACCCTATT+TGG | - | chr4.4:33406286-33406305 | MS.gene052355:intron | 55.0% |
| TTCCAATTGGACGGTGGCCC+TGG | - | chr4.4:33405694-33405713 | MS.gene052355:CDS | 60.0% | |
| GCGCCACCAGCAAACACTCC+AGG | + | chr4.4:33406578-33406597 | None:intergenic | 65.0% | |
| GGCCAGGGCCACCGTCCAAT+TGG | + | chr4.4:33405699-33405718 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 33405471 | 33406802 | 33405471 | ID=MS.gene052355 |
| chr4.4 | mRNA | 33405471 | 33406802 | 33405471 | ID=MS.gene052355.t1;Parent=MS.gene052355 |
| chr4.4 | exon | 33406786 | 33406802 | 33406786 | ID=MS.gene052355.t1.exon1;Parent=MS.gene052355.t1 |
| chr4.4 | CDS | 33406786 | 33406802 | 33406786 | ID=cds.MS.gene052355.t1;Parent=MS.gene052355.t1 |
| chr4.4 | exon | 33405471 | 33405807 | 33405471 | ID=MS.gene052355.t1.exon2;Parent=MS.gene052355.t1 |
| chr4.4 | CDS | 33405471 | 33405807 | 33405471 | ID=cds.MS.gene052355.t1;Parent=MS.gene052355.t1 |
| Gene Sequence |
| Protein sequence |
