AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066242


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066242.t1 MTR_1g099320 96.970 297 9 0 1 297 1 297 0.0 591
MS.gene066242.t1 MTR_1g099350 71.141 298 78 4 1 297 1 291 1.15e-149 421
MS.gene066242.t1 MTR_0027s0260 67.007 294 91 4 5 297 10 298 1.78e-139 395
MS.gene066242.t1 MTR_8g055940 67.586 290 87 4 5 293 10 293 6.70e-137 389
MS.gene066242.t1 MTR_1g099310 65.333 300 97 7 1 297 1 296 1.17e-135 386
MS.gene066242.t1 MTR_5g043550 63.605 294 102 3 1 293 1 290 7.31e-132 376
MS.gene066242.t1 MTR_1g099290 63.194 288 100 6 11 295 12 296 1.19e-123 365
MS.gene066242.t1 MTR_7g116350 61.644 292 96 2 7 297 8 284 4.43e-123 371
MS.gene066242.t1 MTR_8g467650 61.938 289 106 3 7 293 8 294 3.74e-122 352
MS.gene066242.t1 MTR_2g102020 55.732 314 121 5 1 297 1 313 5.24e-120 348
MS.gene066242.t1 MTR_7g116850 55.892 297 125 5 5 297 32 326 7.89e-113 329
MS.gene066242.t1 MTR_2g040740 55.634 284 118 4 14 293 16 295 1.50e-104 308
MS.gene066242.t1 MTR_2g040830 53.684 285 123 5 14 293 16 296 4.76e-98 291
MS.gene066242.t1 MTR_2g040690 52.982 285 125 5 14 293 18 298 1.35e-97 290
MS.gene066242.t1 MTR_6g023340 50.000 286 134 7 18 297 20 302 5.28e-93 278
MS.gene066242.t1 MTR_2g040650 48.421 285 134 6 14 293 16 292 7.04e-86 260
MS.gene066242.t1 MTR_2g040870 49.057 159 77 2 16 174 15 169 4.44e-41 140
MS.gene066242.t1 MTR_2g040880 52.212 113 49 3 186 293 1 113 1.82e-35 125
MS.gene066242.t1 MTR_2g040890 42.683 164 80 6 12 166 8 166 3.91e-35 125
MS.gene066242.t1 MTR_2g040850 45.139 144 70 2 8 145 10 150 5.74e-31 116
MS.gene066242.t1 MTR_2g040900 46.774 124 60 4 180 297 18 141 4.90e-28 106
MS.gene066242.t1 MTR_4g077310 53.933 89 37 4 207 293 3 89 2.31e-23 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066242.t1 AT5G24090 64.000 300 104 3 1 297 4 302 1.35e-142 404

Find 59 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTTTGATTCCTTTATTT+TGG 0.131017 1.1:-71501381 None:intergenic
TTGTGAATTCATGGAATAAA+TGG 0.242224 1.1:+71501770 MS.gene066242:CDS
AACTTGGTTCCACCGAAAAT+TGG 0.244758 1.1:+71501560 MS.gene066242:CDS
CTTTCAATTGGTGGTGGAAT+TGG 0.287500 1.1:+71501406 MS.gene066242:CDS
AGGTTCCTTCGTTGCCATTT+TGG 0.290608 1.1:-71501201 None:intergenic
GCAATCTATTGGGGCCAAAA+TGG 0.300822 1.1:+71501187 MS.gene066242:CDS
GGGTATAGCACTTCTATTAT+TGG 0.304491 1.1:+71501967 MS.gene066242:CDS
TGACCTGCGAGATTCATTTC+AGG 0.305610 1.1:-71501301 None:intergenic
TAACTAACCTTGTGAATTCA+TGG 0.311751 1.1:+71501761 MS.gene066242:CDS
CTTAAAGGGTATAGTAGATA+TGG 0.312136 1.1:+71501601 MS.gene066242:CDS
AATCCACCTTGTCAATATAA+TGG 0.323098 1.1:+71501733 MS.gene066242:CDS
CTGGTGGCATAGCAATCTAT+TGG 0.352724 1.1:+71501176 MS.gene066242:CDS
ATCAAAGATTGCCAAAATAA+AGG 0.357615 1.1:+71501370 MS.gene066242:CDS
GACCGTTACCGAATTTGTTT+AGG 0.359199 1.1:-71501273 None:intergenic
TTGGGCACTGCACTTGAAAC+AGG 0.362436 1.1:+71501676 MS.gene066242:CDS
TGGTGGCATAGCAATCTATT+GGG 0.384033 1.1:+71501177 MS.gene066242:CDS
GAAAATTGGCAACACCTTGC+AGG 0.393402 1.1:+71501574 MS.gene066242:CDS
ATAGCTTTCCTAAACAAATT+CGG 0.403876 1.1:+71501265 MS.gene066242:CDS
TGCCGGAATAAAACCACTTC+CGG 0.411953 1.1:-71501851 None:intergenic
GGTTGCGCATGCTTCGGATA+AGG 0.415599 1.1:-71501221 None:intergenic
CTACTCTTTATCTTCAATTG+AGG 0.422344 1.1:+71501432 MS.gene066242:CDS
AAGTCATCTTCAAGACCATT+AGG 0.427426 1.1:+71501502 MS.gene066242:CDS
TTATCCGAAGCATGCGCAAC+CGG 0.438403 1.1:+71501223 MS.gene066242:CDS
TCTACAGCGGCGGCCGGAAG+TGG 0.441284 1.1:+71501838 MS.gene066242:CDS
TCTAAAACAGCATCACCTAA+TGG 0.445847 1.1:-71501517 None:intergenic
CCGGCATCTACAGCGGCGGC+CGG 0.451172 1.1:+71501832 MS.gene066242:CDS
AGGTTTCATGATTTGCAGAC+TGG 0.456803 1.1:+71501946 MS.gene066242:CDS
GTGCATGAATTTGGAAGTGA+TGG 0.468935 1.1:-71501331 None:intergenic
GCAACAAGAGTGAGGAATAG+AGG 0.475574 1.1:-71501142 None:intergenic
TGGACTAGCACTGTTCCAGC+TGG 0.478277 1.1:+71501790 MS.gene066242:CDS
CTTGTTGCAACTTCCCATGC+TGG 0.478479 1.1:+71501157 MS.gene066242:CDS
TATGGAAGAAAAGTGTATCT+TGG 0.479714 1.1:+71501619 MS.gene066242:CDS
AGTATGGAGGAGTGATGCTT+TGG 0.487463 1.1:+71501920 MS.gene066242:CDS
GACCTGCGAGATTCATTTCA+GGG 0.492299 1.1:-71501300 None:intergenic
GATTGCTATGCCACCAGCAT+GGG 0.506152 1.1:-71501170 None:intergenic
CCGGCCGCCGCTGTAGATGC+CGG 0.519785 1.1:-71501832 None:intergenic
TCTGAAGTCAACACATCTGC+CGG 0.536946 1.1:-71501868 None:intergenic
TTATCTGGAATTGGACATTG+AGG 0.543911 1.1:-71501649 None:intergenic
GGTTTCATGATTTGCAGACT+GGG 0.544792 1.1:+71501947 MS.gene066242:CDS
GAGGAGTGATGCTTTGGTCA+AGG 0.544888 1.1:+71501926 MS.gene066242:CDS
TTATGGAACACTTTCTTGGG+TGG 0.560429 1.1:+71501478 MS.gene066242:CDS
ACCCCTGAAATGAATCTCGC+AGG 0.567411 1.1:+71501298 MS.gene066242:CDS
TTCCTAAACAAATTCGGTAA+CGG 0.579160 1.1:+71501271 MS.gene066242:CDS
AGGGCTGCCGGCATCTACAG+CGG 0.585171 1.1:+71501825 MS.gene066242:CDS
TGGGGCCAAAATGGCAACGA+AGG 0.591522 1.1:+71501196 MS.gene066242:CDS
AGATTGCTATGCCACCAGCA+TGG 0.592121 1.1:-71501171 None:intergenic
TTTCCATTATATTGACAAGG+TGG 0.592596 1.1:-71501736 None:intergenic
GTTGCAACTTCCCATGCTGG+TGG 0.594762 1.1:+71501160 MS.gene066242:CDS
GGACTAGCACTGTTCCAGCT+GGG 0.601285 1.1:+71501791 MS.gene066242:CDS
GCTGCCGGCATCTACAGCGG+CGG 0.604171 1.1:+71501828 MS.gene066242:CDS
TTAGGAAAGCTATGTTAACA+TGG 0.604430 1.1:-71501255 None:intergenic
ATTTATTCCATGAATTCACA+AGG 0.612674 1.1:-71501768 None:intergenic
ACCCTTTAAGAAAACCTGCA+AGG 0.616480 1.1:-71501588 None:intergenic
ACCTGCGAGATTCATTTCAG+GGG 0.619333 1.1:-71501299 None:intergenic
ATGTTTCCATTATATTGACA+AGG 0.620396 1.1:-71501739 None:intergenic
TGCATGAATTTGGAAGTGAT+GGG 0.620811 1.1:-71501330 None:intergenic
TAGGAAAGCTATGTTAACAT+GGG 0.626283 1.1:-71501254 None:intergenic
GGGAAGTTGCAACAAGAGTG+AGG 0.646970 1.1:-71501150 None:intergenic
GGTGGCATAGCAATCTATTG+GGG 0.654630 1.1:+71501178 MS.gene066242:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAGAAGTCAAAAAAGTA+TGG + chr1.1:71501904-71501923 MS.gene066242:CDS 20.0%
!!! AAACTTTGATTCCTTTATTT+TGG - chr1.1:71501384-71501403 None:intergenic 20.0%
!!! ATAGATTTTGATATTGAACT+TGG + chr1.1:71501544-71501563 MS.gene066242:CDS 20.0%
!!! ATCAAAGTTTTACTTTCAAT+TGG + chr1.1:71501394-71501413 MS.gene066242:CDS 20.0%
!!! GAAACGTTTCAAAATTTTTA+TGG + chr1.1:71501461-71501480 MS.gene066242:CDS 20.0%
! ATAGCTTTCCTAAACAAATT+CGG + chr1.1:71501265-71501284 MS.gene066242:CDS 25.0%
! ATCAAAGATTGCCAAAATAA+AGG + chr1.1:71501370-71501389 MS.gene066242:CDS 25.0%
! ATGTTTCCATTATATTGACA+AGG - chr1.1:71501742-71501761 None:intergenic 25.0%
! ATTTATTCCATGAATTCACA+AGG - chr1.1:71501771-71501790 None:intergenic 25.0%
! TTGTGAATTCATGGAATAAA+TGG + chr1.1:71501770-71501789 MS.gene066242:CDS 25.0%
!! AAAGTTTTACTTTCAATTGG+TGG + chr1.1:71501397-71501416 MS.gene066242:CDS 25.0%
!! ATTTTTATGGAACACTTTCT+TGG + chr1.1:71501474-71501493 MS.gene066242:CDS 25.0%
!! CCAATTCCAGATAAATTTTT+GGG + chr1.1:71501658-71501677 MS.gene066242:CDS 25.0%
!! TCCAATTCCAGATAAATTTT+TGG + chr1.1:71501657-71501676 MS.gene066242:CDS 25.0%
!! TTTTTATGGAACACTTTCTT+GGG + chr1.1:71501475-71501494 MS.gene066242:CDS 25.0%
!!! TTTTTTGACTTCTTAATCAC+AGG - chr1.1:71501901-71501920 None:intergenic 25.0%
AATCCACCTTGTCAATATAA+TGG + chr1.1:71501733-71501752 MS.gene066242:CDS 30.0%
CTACTCTTTATCTTCAATTG+AGG + chr1.1:71501432-71501451 MS.gene066242:CDS 30.0%
TAACTAACCTTGTGAATTCA+TGG + chr1.1:71501761-71501780 MS.gene066242:CDS 30.0%
TAGGAAAGCTATGTTAACAT+GGG - chr1.1:71501257-71501276 None:intergenic 30.0%
TTAGGAAAGCTATGTTAACA+TGG - chr1.1:71501258-71501277 None:intergenic 30.0%
TTCCTAAACAAATTCGGTAA+CGG + chr1.1:71501271-71501290 MS.gene066242:CDS 30.0%
TTTCCATTATATTGACAAGG+TGG - chr1.1:71501739-71501758 None:intergenic 30.0%
! AAGAAGTCAAAAAAGTATGG+AGG + chr1.1:71501907-71501926 MS.gene066242:CDS 30.0%
! CCCAAAAATTTATCTGGAAT+TGG - chr1.1:71501661-71501680 None:intergenic 30.0%
! CTGAATTTTGTGCATGAATT+TGG - chr1.1:71501343-71501362 None:intergenic 30.0%
! CTTAAAGGGTATAGTAGATA+TGG + chr1.1:71501601-71501620 MS.gene066242:CDS 30.0%
! TATGGAAGAAAAGTGTATCT+TGG + chr1.1:71501619-71501638 MS.gene066242:CDS 30.0%
!!! AGGTCTTTTTGACTTTGTTT+GGG + chr1.1:71501696-71501715 MS.gene066242:CDS 30.0%
AAGTCATCTTCAAGACCATT+AGG + chr1.1:71501502-71501521 MS.gene066242:CDS 35.0%
GGGTATAGCACTTCTATTAT+TGG + chr1.1:71501967-71501986 MS.gene066242:CDS 35.0%
TGCATGAATTTGGAAGTGAT+GGG - chr1.1:71501333-71501352 None:intergenic 35.0%
! ACCTTGCAGGTTTTCTTAAA+GGG + chr1.1:71501587-71501606 MS.gene066242:CDS 35.0%
! GTTTTACTTTCAATTGGTGG+TGG + chr1.1:71501400-71501419 MS.gene066242:CDS 35.0%
! TCTAAAACAGCATCACCTAA+TGG - chr1.1:71501520-71501539 None:intergenic 35.0%
! TTATCTGGAATTGGACATTG+AGG - chr1.1:71501652-71501671 None:intergenic 35.0%
!! CAGCTGGGAAGATATTTTTA+GGG + chr1.1:71501806-71501825 MS.gene066242:CDS 35.0%
!!! CAGGTCTTTTTGACTTTGTT+TGG + chr1.1:71501695-71501714 MS.gene066242:CDS 35.0%
!!! GTTAACATGGGAGTATTTTC+CGG - chr1.1:71501245-71501264 None:intergenic 35.0%
!!! TTAGGTGATGCTGTTTTAGA+TGG + chr1.1:71501520-71501539 MS.gene066242:CDS 35.0%
AACTTGGTTCCACCGAAAAT+TGG + chr1.1:71501560-71501579 MS.gene066242:CDS 40.0%
CCTAAAAATATCTTCCCAGC+TGG - chr1.1:71501808-71501827 None:intergenic 40.0%
GCAGTGCCCAAAAATTTATC+TGG - chr1.1:71501667-71501686 None:intergenic 40.0%
GTGCATGAATTTGGAAGTGA+TGG - chr1.1:71501334-71501353 None:intergenic 40.0%
TGGTGGCATAGCAATCTATT+GGG + chr1.1:71501177-71501196 MS.gene066242:CDS 40.0%
TTATGGAACACTTTCTTGGG+TGG + chr1.1:71501478-71501497 MS.gene066242:CDS 40.0%
! ACCCTTTAAGAAAACCTGCA+AGG - chr1.1:71501591-71501610 None:intergenic 40.0%
! AGGTTTCATGATTTGCAGAC+TGG + chr1.1:71501946-71501965 MS.gene066242:CDS 40.0%
! CACCTTGCAGGTTTTCTTAA+AGG + chr1.1:71501586-71501605 MS.gene066242:CDS 40.0%
! CCAGCTGGGAAGATATTTTT+AGG + chr1.1:71501805-71501824 MS.gene066242:CDS 40.0%
! GACCGTTACCGAATTTGTTT+AGG - chr1.1:71501276-71501295 None:intergenic 40.0%
! GGTTTCATGATTTGCAGACT+GGG + chr1.1:71501947-71501966 MS.gene066242:CDS 40.0%
!! CTTTCAATTGGTGGTGGAAT+TGG + chr1.1:71501406-71501425 MS.gene066242:CDS 40.0%
!!! GAAGATATTTTTAGGGCTGC+CGG + chr1.1:71501813-71501832 MS.gene066242:CDS 40.0%
!!! TGCAAGGTGTTGCCAATTTT+CGG - chr1.1:71501575-71501594 None:intergenic 40.0%
ACCTGCGAGATTCATTTCAG+GGG - chr1.1:71501302-71501321 None:intergenic 45.0%
AGTATGGAGGAGTGATGCTT+TGG + chr1.1:71501920-71501939 MS.gene066242:CDS 45.0%
CTGGTGGCATAGCAATCTAT+TGG + chr1.1:71501176-71501195 MS.gene066242:CDS 45.0%
GAAAATTGGCAACACCTTGC+AGG + chr1.1:71501574-71501593 MS.gene066242:CDS 45.0%
GACCTGCGAGATTCATTTCA+GGG - chr1.1:71501303-71501322 None:intergenic 45.0%
GCAACAAGAGTGAGGAATAG+AGG - chr1.1:71501145-71501164 None:intergenic 45.0%
GCAATCTATTGGGGCCAAAA+TGG + chr1.1:71501187-71501206 MS.gene066242:CDS 45.0%
GGTGGCATAGCAATCTATTG+GGG + chr1.1:71501178-71501197 MS.gene066242:CDS 45.0%
TGACCTGCGAGATTCATTTC+AGG - chr1.1:71501304-71501323 None:intergenic 45.0%
TGCCGGAATAAAACCACTTC+CGG - chr1.1:71501854-71501873 None:intergenic 45.0%
! TCTGAAGTCAACACATCTGC+CGG - chr1.1:71501871-71501890 None:intergenic 45.0%
!! AGGTTCCTTCGTTGCCATTT+TGG - chr1.1:71501204-71501223 None:intergenic 45.0%
!!! AAGGTGTTGCCAATTTTCGG+TGG - chr1.1:71501572-71501591 None:intergenic 45.0%
ACCCCTGAAATGAATCTCGC+AGG + chr1.1:71501298-71501317 MS.gene066242:CDS 50.0%
AGATTGCTATGCCACCAGCA+TGG - chr1.1:71501174-71501193 None:intergenic 50.0%
CTTGTTGCAACTTCCCATGC+TGG + chr1.1:71501157-71501176 MS.gene066242:CDS 50.0%
GAGGAGTGATGCTTTGGTCA+AGG + chr1.1:71501926-71501945 MS.gene066242:CDS 50.0%
GATTGCTATGCCACCAGCAT+GGG - chr1.1:71501173-71501192 None:intergenic 50.0%
GGGAAGTTGCAACAAGAGTG+AGG - chr1.1:71501153-71501172 None:intergenic 50.0%
TTATCCGAAGCATGCGCAAC+CGG + chr1.1:71501223-71501242 MS.gene066242:CDS 50.0%
! TTGGGCACTGCACTTGAAAC+AGG + chr1.1:71501676-71501695 MS.gene066242:CDS 50.0%
! TTTTCCGGTTGCGCATGCTT+CGG - chr1.1:71501230-71501249 None:intergenic 50.0%
!!! GGCCGGAAGTGGTTTTATTC+CGG + chr1.1:71501849-71501868 MS.gene066242:CDS 50.0%
GGTTGCGCATGCTTCGGATA+AGG - chr1.1:71501224-71501243 None:intergenic 55.0%
GTTGCAACTTCCCATGCTGG+TGG + chr1.1:71501160-71501179 MS.gene066242:CDS 55.0%
TGGGGCCAAAATGGCAACGA+AGG + chr1.1:71501196-71501215 MS.gene066242:CDS 55.0%
! GGACTAGCACTGTTCCAGCT+GGG + chr1.1:71501791-71501810 MS.gene066242:CDS 55.0%
!! TGGACTAGCACTGTTCCAGC+TGG + chr1.1:71501790-71501809 MS.gene066242:CDS 55.0%
AGGGCTGCCGGCATCTACAG+CGG + chr1.1:71501825-71501844 MS.gene066242:CDS 65.0%
GCTGCCGGCATCTACAGCGG+CGG + chr1.1:71501828-71501847 MS.gene066242:CDS 70.0%
TCTACAGCGGCGGCCGGAAG+TGG + chr1.1:71501838-71501857 MS.gene066242:CDS 70.0%
CCGGCATCTACAGCGGCGGC+CGG + chr1.1:71501832-71501851 MS.gene066242:CDS 75.0%
CCGGCCGCCGCTGTAGATGC+CGG - chr1.1:71501835-71501854 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 71501106 71501999 71501106 ID=MS.gene066242
chr1.1 mRNA 71501106 71501999 71501106 ID=MS.gene066242.t1;Parent=MS.gene066242
chr1.1 exon 71501106 71501999 71501106 ID=MS.gene066242.t1.exon1;Parent=MS.gene066242.t1
chr1.1 CDS 71501106 71501999 71501106 ID=cds.MS.gene066242.t1;Parent=MS.gene066242.t1
Gene Sequence

>MS.gene066242

ATGGCAAAAAACTCTCACACTTTGTTGTCTCTTATTCCTCTATTCCTCACTCTTGTTGCAACTTCCCATGCTGGTGGCATAGCAATCTATTGGGGCCAAAATGGCAACGAAGGAACCTTATCCGAAGCATGCGCAACCGGAAAATACTCCCATGTTAACATAGCTTTCCTAAACAAATTCGGTAACGGTCAAACCCCTGAAATGAATCTCGCAGGTCACTGCAACCCATCACTTCCAAATTCATGCACAAAATTCAGCTCAGAAATCAAAGATTGCCAAAATAAAGGAATCAAAGTTTTACTTTCAATTGGTGGTGGAATTGGAAGCTACTCTTTATCTTCAATTGAGGATGCAAGAAACGTTTCAAAATTTTTATGGAACACTTTCTTGGGTGGCAAGTCATCTTCAAGACCATTAGGTGATGCTGTTTTAGATGGAATAGATTTTGATATTGAACTTGGTTCCACCGAAAATTGGCAACACCTTGCAGGTTTTCTTAAAGGGTATAGTAGATATGGAAGAAAAGTGTATCTTGGTGCAGCTCCTCAATGTCCAATTCCAGATAAATTTTTGGGCACTGCACTTGAAACAGGTCTTTTTGACTTTGTTTGGGTACAATTTTACAATAATCCACCTTGTCAATATAATGGAAACATAACTAACCTTGTGAATTCATGGAATAAATGGACTAGCACTGTTCCAGCTGGGAAGATATTTTTAGGGCTGCCGGCATCTACAGCGGCGGCCGGAAGTGGTTTTATTCCGGCAGATGTGTTGACTTCAGAAATTTTACCTGTGATTAAGAAGTCAAAAAAGTATGGAGGAGTGATGCTTTGGTCAAGGTTTCATGATTTGCAGACTGGGTATAGCACTTCTATTATTGGAAGCGTGTGA

Protein sequence

>MS.gene066242.t1

MAKNSHTLLSLIPLFLTLVATSHAGGIAIYWGQNGNEGTLSEACATGKYSHVNIAFLNKFGNGQTPEMNLAGHCNPSLPNSCTKFSSEIKDCQNKGIKVLLSIGGGIGSYSLSSIEDARNVSKFLWNTFLGGKSSSRPLGDAVLDGIDFDIELGSTENWQHLAGFLKGYSRYGRKVYLGAAPQCPIPDKFLGTALETGLFDFVWVQFYNNPPCQYNGNITNLVNSWNKWTSTVPAGKIFLGLPASTAAAGSGFIPADVLTSEILPVIKKSKKYGGVMLWSRFHDLQTGYSTSIIGSV