Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066719.t1 | XP_024631911.1 | 93.4 | 181 | 11 | 1 | 52 | 231 | 321 | 501 | 6.10E-90 | 340.5 |
| MS.gene066719.t1 | XP_024631911.1 | 86.4 | 44 | 6 | 0 | 2 | 45 | 3 | 46 | 8.70E-12 | 80.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066719.t1 | A0A3Q7XGP6 | 73.1 | 182 | 44 | 2 | 51 | 231 | 41 | 218 | 7.5e-66 | 260.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050719 | MS.gene066719 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene050721 | MS.gene066719 | 0.803525 | 3.34E-49 | -1.69E-46 |
| MS.gene055603 | MS.gene066719 | 0.807144 | 5.83E-50 | -1.69E-46 |
| MS.gene059774 | MS.gene066719 | 0.800789 | 1.22E-48 | -1.69E-46 |
| MS.gene060697 | MS.gene066719 | 0.802157 | 6.41E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066719.t1 | MTR_0125s0060 | 93.750 | 48 | 3 | 0 | 184 | 231 | 1 | 48 | 1.30e-25 | 97.4 |
| MS.gene066719.t1 | MTR_2g035110 | 79.167 | 48 | 10 | 0 | 184 | 231 | 1 | 48 | 1.04e-18 | 79.7 |
| MS.gene066719.t1 | MTR_4g029740 | 39.560 | 91 | 53 | 2 | 57 | 146 | 112 | 201 | 8.44e-13 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 53 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCCATCTGGTTTCCCTTT+TGG | 0.170423 | 4.2:-21002058 | None:intergenic |
| TGAAACAGTTGGAGGTTAAA+TGG | 0.204653 | 4.2:+21001786 | MS.gene066719:CDS |
| AATTGACAATGATATTTATA+TGG | 0.247121 | 4.2:+21002263 | MS.gene066719:CDS |
| AACAAGAAGGGGCGCATATT+TGG | 0.259894 | 4.2:+21002303 | MS.gene066719:CDS |
| TCTAAGCACTCTAGCAGATA+TGG | 0.281662 | 4.2:-21001894 | None:intergenic |
| TCCAAAATCAAAACCTTCAT+TGG | 0.305327 | 4.2:+21001107 | MS.gene066719:CDS |
| AGAGGCAGGGGAAGAGGTTA+AGG | 0.348872 | 4.2:+21001261 | MS.gene066719:CDS |
| TTGAGCCGATTCTCCTCATA+TGG | 0.367579 | 4.2:-21001006 | None:intergenic |
| CTATGACTAATAAACAAAAT+AGG | 0.370881 | 4.2:+21001834 | MS.gene066719:CDS |
| AAGGGGCGCATATTTGGATT+AGG | 0.373630 | 4.2:+21002309 | MS.gene066719:CDS |
| GATATTTATATGGAAATTGT+TGG | 0.391812 | 4.2:+21002273 | MS.gene066719:CDS |
| AGAGAATCATAGAGTGATAA+AGG | 0.396522 | 4.2:+21001038 | MS.gene066719:CDS |
| GTTTCTCAAACGTTTGAGTT+TGG | 0.397323 | 4.2:-21002197 | None:intergenic |
| GACAAACTTCATAGACCAAA+AGG | 0.414225 | 4.2:+21002043 | MS.gene066719:CDS |
| GAAACAGTTGGAGGTTAAAT+GGG | 0.415071 | 4.2:+21001787 | MS.gene066719:CDS |
| GTGCGTAGTGGTCATGATAA+AGG | 0.417897 | 4.2:+21001743 | MS.gene066719:CDS |
| ACAAGCAGAGGCAGGGGAAG+AGG | 0.419127 | 4.2:+21001255 | MS.gene066719:CDS |
| GACCACTACGCACCTTGAAA+AGG | 0.438590 | 4.2:-21001733 | None:intergenic |
| CAAAATAGGCAGTCTAAAGT+TGG | 0.454140 | 4.2:+21001848 | MS.gene066719:CDS |
| GTTTCATTAAACCACCCATC+TGG | 0.467331 | 4.2:-21002070 | None:intergenic |
| AGATTATCACTGACCAATGA+AGG | 0.469208 | 4.2:-21001120 | None:intergenic |
| CACCACCATATGAGGAGAAT+CGG | 0.469337 | 4.2:+21001001 | MS.gene066719:CDS |
| TGTCAGGTACAAGCAGAGGC+AGG | 0.470125 | 4.2:+21001247 | MS.gene066719:intron |
| TTAGAGAGTGGAATTGGATA+GGG | 0.471643 | 4.2:+21001997 | MS.gene066719:intron |
| CGTCTCTCTCAAGATCTTCA+CGG | 0.472964 | 4.2:+21001075 | MS.gene066719:CDS |
| TTTAGAGAGTGGAATTGGAT+AGG | 0.476342 | 4.2:+21001996 | MS.gene066719:intron |
| TTAAATGGGAAGAAGAGAAA+TGG | 0.501205 | 4.2:+21001801 | MS.gene066719:CDS |
| TCAGGTACAAGCAGAGGCAG+GGG | 0.505090 | 4.2:+21001249 | MS.gene066719:intron |
| ACCACTACGCACCTTGAAAA+GGG | 0.509216 | 4.2:-21001732 | None:intergenic |
| TGTTGGAGGCATTAACAAGA+AGG | 0.515610 | 4.2:+21002290 | MS.gene066719:CDS |
| ATTTATATGGAAATTGTTGG+AGG | 0.522832 | 4.2:+21002276 | MS.gene066719:CDS |
| TCATTGTCAATTTCTTCAGT+AGG | 0.525205 | 4.2:-21002252 | None:intergenic |
| GTCAGGTACAAGCAGAGGCA+GGG | 0.534453 | 4.2:+21001248 | MS.gene066719:intron |
| GTGGTCATGATAAAGGTGAA+TGG | 0.542038 | 4.2:+21001750 | MS.gene066719:CDS |
| AGCGATGACACCACCATATG+AGG | 0.554141 | 4.2:+21000993 | None:intergenic |
| GTTGGAGGCATTAACAAGAA+GGG | 0.555037 | 4.2:+21002291 | MS.gene066719:CDS |
| ACAAACTTCATAGACCAAAA+GGG | 0.556595 | 4.2:+21002044 | MS.gene066719:CDS |
| TGGTGACAACTTGAAACAGT+TGG | 0.561040 | 4.2:+21001775 | MS.gene066719:CDS |
| AGACCAAAAGGGAAACCAGA+TGG | 0.568337 | 4.2:+21002055 | MS.gene066719:CDS |
| GACCAAAAGGGAAACCAGAT+GGG | 0.568542 | 4.2:+21002056 | MS.gene066719:CDS |
| TTGGAGGCATTAACAAGAAG+GGG | 0.580609 | 4.2:+21002292 | MS.gene066719:CDS |
| CATGATAAAGGTGAATGGAT+TGG | 0.585265 | 4.2:+21001755 | MS.gene066719:CDS |
| TCGTAAATCTCAAACCATGT+TGG | 0.585577 | 4.2:-21002022 | None:intergenic |
| TGACAACTTGAAACAGTTGG+AGG | 0.589439 | 4.2:+21001778 | MS.gene066719:CDS |
| AGCCGATTCTCCTCATATGG+TGG | 0.602554 | 4.2:-21001003 | None:intergenic |
| GTTTGTAGGCTAAATTCAAG+TGG | 0.608599 | 4.2:+21001642 | MS.gene066719:intron |
| AAAAGAGCTTTAATAGTAGG+GGG | 0.614401 | 4.2:+21001690 | MS.gene066719:CDS |
| TAGATGTCAGGTACAAGCAG+AGG | 0.622801 | 4.2:+21001243 | MS.gene066719:intron |
| GGACACAATGTGCACAGTGG+AGG | 0.650149 | 4.2:+21001869 | MS.gene066719:CDS |
| AATTGGATAGGGAACCAACA+TGG | 0.651250 | 4.2:+21002008 | MS.gene066719:CDS |
| CAAAAGGGAAACCAGATGGG+TGG | 0.669221 | 4.2:+21002059 | MS.gene066719:CDS |
| TTTGAGAAACACTCTCAAAG+CGG | 0.702666 | 4.2:+21002210 | MS.gene066719:CDS |
| GTTGGACACAATGTGCACAG+TGG | 0.740896 | 4.2:+21001866 | MS.gene066719:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTATGATTTCTTATAGT+TGG | + | chr4.2:21001400-21001419 | MS.gene066719:intron | 15.0% |
| !! | AATTGACAATGATATTTATA+TGG | + | chr4.2:21002263-21002282 | MS.gene066719:CDS | 15.0% |
| !! | TCATATCATATAAATCAAAT+AGG | - | chr4.2:21001547-21001566 | None:intergenic | 15.0% |
| !! | ACAAATGTAGTTACATAAAT+AGG | + | chr4.2:21001342-21001361 | MS.gene066719:intron | 20.0% |
| !! | CATAATTTCACAATATTCAT+TGG | - | chr4.2:21001388-21001407 | None:intergenic | 20.0% |
| !! | CTATGACTAATAAACAAAAT+AGG | + | chr4.2:21001834-21001853 | MS.gene066719:CDS | 20.0% |
| !! | GATATTTATATGGAAATTGT+TGG | + | chr4.2:21002273-21002292 | MS.gene066719:CDS | 20.0% |
| !!! | TAAAAAGAGCTTTAATAGTA+GGG | + | chr4.2:21001688-21001707 | MS.gene066719:CDS | 20.0% |
| !!! | TTAAAAAGAGCTTTAATAGT+AGG | + | chr4.2:21001687-21001706 | MS.gene066719:CDS | 20.0% |
| ! | AATTTCCTATCATGTAGAAT+AGG | + | chr4.2:21001309-21001328 | MS.gene066719:intron | 25.0% |
| ! | ATGAAGCTATGTATTTGAAA+AGG | - | chr4.2:21001611-21001630 | None:intergenic | 25.0% |
| ! | ATTTATATGGAAATTGTTGG+AGG | + | chr4.2:21002276-21002295 | MS.gene066719:CDS | 25.0% |
| ! | TATTTCCTATTCTACATGAT+AGG | - | chr4.2:21001317-21001336 | None:intergenic | 25.0% |
| !! | AAAAAGAGCTTTAATAGTAG+GGG | + | chr4.2:21001689-21001708 | MS.gene066719:CDS | 25.0% |
| !! | ATTAAAAAATGCCCTTTTCA+AGG | + | chr4.2:21001721-21001740 | MS.gene066719:CDS | 25.0% |
| !!! | CATACTAATTTGTTCTTTGT+TGG | + | chr4.2:21001967-21001986 | MS.gene066719:intron | 25.0% |
| !!! | CATGACATTTTTTTGTTTGT+AGG | + | chr4.2:21001628-21001647 | MS.gene066719:intron | 25.0% |
| !!! | TAATAGTAACTCTTTTTTGC+AGG | + | chr4.2:21002167-21002186 | MS.gene066719:intron | 25.0% |
| ACAAACTTCATAGACCAAAA+GGG | + | chr4.2:21002044-21002063 | MS.gene066719:CDS | 30.0% | |
| AGAGAATCATAGAGTGATAA+AGG | + | chr4.2:21001038-21001057 | MS.gene066719:CDS | 30.0% | |
| ATTAGTCCTAGTAGAGTATA+TGG | - | chr4.2:21001197-21001216 | None:intergenic | 30.0% | |
| TCATTGTCAATTTCTTCAGT+AGG | - | chr4.2:21002255-21002274 | None:intergenic | 30.0% | |
| TCCAAAATCAAAACCTTCAT+TGG | + | chr4.2:21001107-21001126 | MS.gene066719:CDS | 30.0% | |
| TTAAATGGGAAGAAGAGAAA+TGG | + | chr4.2:21001801-21001820 | MS.gene066719:CDS | 30.0% | |
| ! | AAAAGAGCTTTAATAGTAGG+GGG | + | chr4.2:21001690-21001709 | MS.gene066719:CDS | 30.0% |
| ! | ATGAAGCTTCATTGTTTCTT+AGG | + | chr4.2:21001570-21001589 | MS.gene066719:intron | 30.0% |
| ! | TAGAGTGCTTAGAAAAAGAA+TGG | + | chr4.2:21001904-21001923 | MS.gene066719:CDS | 30.0% |
| !! | ATGTGTTGTTTATAGATGTC+AGG | + | chr4.2:21001231-21001250 | MS.gene066719:intron | 30.0% |
| !!! | ACCAATGAAGGTTTTGATTT+TGG | - | chr4.2:21001111-21001130 | None:intergenic | 30.0% |
| !!! | TATGTTTTAGAGAGTGGAAT+TGG | + | chr4.2:21001991-21002010 | MS.gene066719:intron | 30.0% |
| AAAAGAGCAGCAATTCCAAT+AGG | - | chr4.2:21001522-21001541 | None:intergenic | 35.0% | |
| AGATTATCACTGACCAATGA+AGG | - | chr4.2:21001123-21001142 | None:intergenic | 35.0% | |
| CATGATAAAGGTGAATGGAT+TGG | + | chr4.2:21001755-21001774 | MS.gene066719:CDS | 35.0% | |
| GAAACAGTTGGAGGTTAAAT+GGG | + | chr4.2:21001787-21001806 | MS.gene066719:CDS | 35.0% | |
| GACAAACTTCATAGACCAAA+AGG | + | chr4.2:21002043-21002062 | MS.gene066719:CDS | 35.0% | |
| GTTTGTAGGCTAAATTCAAG+TGG | + | chr4.2:21001642-21001661 | MS.gene066719:intron | 35.0% | |
| TATGTGCAACATGATCCTAT+TGG | + | chr4.2:21001504-21001523 | MS.gene066719:intron | 35.0% | |
| TCGTAAATCTCAAACCATGT+TGG | - | chr4.2:21002025-21002044 | None:intergenic | 35.0% | |
| TGAAACAGTTGGAGGTTAAA+TGG | + | chr4.2:21001786-21001805 | MS.gene066719:CDS | 35.0% | |
| TTAGAGAGTGGAATTGGATA+GGG | + | chr4.2:21001997-21002016 | MS.gene066719:intron | 35.0% | |
| TTGAGTTTCAATTTGAGTGC+TGG | + | chr4.2:21001171-21001190 | MS.gene066719:intron | 35.0% | |
| TTTAGAGAGTGGAATTGGAT+AGG | + | chr4.2:21001996-21002015 | MS.gene066719:intron | 35.0% | |
| TTTGAGAAACACTCTCAAAG+CGG | + | chr4.2:21002210-21002229 | MS.gene066719:CDS | 35.0% | |
| ! | CAAAATAGGCAGTCTAAAGT+TGG | + | chr4.2:21001848-21001867 | MS.gene066719:CDS | 35.0% |
| ! | GTTTCTCAAACGTTTGAGTT+TGG | - | chr4.2:21002200-21002219 | None:intergenic | 35.0% |
| !!! | AGGTTTTGATTTTGGAGAAG+TGG | - | chr4.2:21001103-21001122 | None:intergenic | 35.0% |
| !!! | GTTGGTTATGTTTTAGAGAG+TGG | + | chr4.2:21001985-21002004 | MS.gene066719:intron | 35.0% |
| AATTGGATAGGGAACCAACA+TGG | + | chr4.2:21002008-21002027 | MS.gene066719:CDS | 40.0% | |
| CTTTAATAGTAGGGGGTCTT+TGG | + | chr4.2:21001697-21001716 | MS.gene066719:CDS | 40.0% | |
| GTGGTCATGATAAAGGTGAA+TGG | + | chr4.2:21001750-21001769 | MS.gene066719:CDS | 40.0% | |
| GTTTCATTAAACCACCCATC+TGG | - | chr4.2:21002073-21002092 | None:intergenic | 40.0% | |
| TCTAAGCACTCTAGCAGATA+TGG | - | chr4.2:21001897-21001916 | None:intergenic | 40.0% | |
| TGACAACTTGAAACAGTTGG+AGG | + | chr4.2:21001778-21001797 | MS.gene066719:CDS | 40.0% | |
| TGGTGACAACTTGAAACAGT+TGG | + | chr4.2:21001775-21001794 | MS.gene066719:CDS | 40.0% | |
| !! | GTTGGAGGCATTAACAAGAA+GGG | + | chr4.2:21002291-21002310 | MS.gene066719:CDS | 40.0% |
| !! | TGTTGGAGGCATTAACAAGA+AGG | + | chr4.2:21002290-21002309 | MS.gene066719:CDS | 40.0% |
| !! | TTGGAGGCATTAACAAGAAG+GGG | + | chr4.2:21002292-21002311 | MS.gene066719:CDS | 40.0% |
| ACCACTACGCACCTTGAAAA+GGG | - | chr4.2:21001735-21001754 | None:intergenic | 45.0% | |
| AGACCAAAAGGGAAACCAGA+TGG | + | chr4.2:21002055-21002074 | MS.gene066719:CDS | 45.0% | |
| CACCACCATATGAGGAGAAT+CGG | + | chr4.2:21001001-21001020 | MS.gene066719:CDS | 45.0% | |
| CGTCTCTCTCAAGATCTTCA+CGG | + | chr4.2:21001075-21001094 | MS.gene066719:CDS | 45.0% | |
| GACCAAAAGGGAAACCAGAT+GGG | + | chr4.2:21002056-21002075 | MS.gene066719:CDS | 45.0% | |
| GTGCGTAGTGGTCATGATAA+AGG | + | chr4.2:21001743-21001762 | MS.gene066719:CDS | 45.0% | |
| TAGATGTCAGGTACAAGCAG+AGG | + | chr4.2:21001243-21001262 | MS.gene066719:intron | 45.0% | |
| TGCTGGCCATATACTCTACT+AGG | + | chr4.2:21001188-21001207 | MS.gene066719:intron | 45.0% | |
| ! | AACAAGAAGGGGCGCATATT+TGG | + | chr4.2:21002303-21002322 | MS.gene066719:CDS | 45.0% |
| ! | AAGGGGCGCATATTTGGATT+AGG | + | chr4.2:21002309-21002328 | MS.gene066719:CDS | 45.0% |
| ! | TTGAGCCGATTCTCCTCATA+TGG | - | chr4.2:21001009-21001028 | None:intergenic | 45.0% |
| CAAAAGGGAAACCAGATGGG+TGG | + | chr4.2:21002059-21002078 | MS.gene066719:CDS | 50.0% | |
| GACCACTACGCACCTTGAAA+AGG | - | chr4.2:21001736-21001755 | None:intergenic | 50.0% | |
| GTTGGACACAATGTGCACAG+TGG | + | chr4.2:21001866-21001885 | MS.gene066719:CDS | 50.0% | |
| ! | AGCCGATTCTCCTCATATGG+TGG | - | chr4.2:21001006-21001025 | None:intergenic | 50.0% |
| !! | CACCCATCTGGTTTCCCTTT+TGG | - | chr4.2:21002061-21002080 | None:intergenic | 50.0% |
| AGAGGCAGGGGAAGAGGTTA+AGG | + | chr4.2:21001261-21001280 | MS.gene066719:CDS | 55.0% | |
| GGACACAATGTGCACAGTGG+AGG | + | chr4.2:21001869-21001888 | MS.gene066719:CDS | 55.0% | |
| GTCAGGTACAAGCAGAGGCA+GGG | + | chr4.2:21001248-21001267 | MS.gene066719:intron | 55.0% | |
| TCAGGTACAAGCAGAGGCAG+GGG | + | chr4.2:21001249-21001268 | MS.gene066719:intron | 55.0% | |
| TGTCAGGTACAAGCAGAGGC+AGG | + | chr4.2:21001247-21001266 | MS.gene066719:intron | 55.0% | |
| !! | GCCCTTTTCAAGGTGCGTAG+TGG | + | chr4.2:21001731-21001750 | MS.gene066719:CDS | 55.0% |
| ACAAGCAGAGGCAGGGGAAG+AGG | + | chr4.2:21001255-21001274 | MS.gene066719:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 21000997 | 21002338 | 21000997 | ID=MS.gene066719 |
| chr4.2 | mRNA | 21000997 | 21002338 | 21000997 | ID=MS.gene066719.t1;Parent=MS.gene066719 |
| chr4.2 | exon | 21000997 | 21001128 | 21000997 | ID=MS.gene066719.t1.exon1;Parent=MS.gene066719.t1 |
| chr4.2 | CDS | 21000997 | 21001128 | 21000997 | ID=cds.MS.gene066719.t1;Parent=MS.gene066719.t1 |
| chr4.2 | exon | 21001253 | 21001282 | 21001253 | ID=MS.gene066719.t1.exon2;Parent=MS.gene066719.t1 |
| chr4.2 | CDS | 21001253 | 21001282 | 21001253 | ID=cds.MS.gene066719.t1;Parent=MS.gene066719.t1 |
| chr4.2 | exon | 21001650 | 21001925 | 21001650 | ID=MS.gene066719.t1.exon3;Parent=MS.gene066719.t1 |
| chr4.2 | CDS | 21001650 | 21001925 | 21001650 | ID=cds.MS.gene066719.t1;Parent=MS.gene066719.t1 |
| chr4.2 | exon | 21002001 | 21002108 | 21002001 | ID=MS.gene066719.t1.exon4;Parent=MS.gene066719.t1 |
| chr4.2 | CDS | 21002001 | 21002108 | 21002001 | ID=cds.MS.gene066719.t1;Parent=MS.gene066719.t1 |
| chr4.2 | exon | 21002189 | 21002338 | 21002189 | ID=MS.gene066719.t1.exon5;Parent=MS.gene066719.t1 |
| chr4.2 | CDS | 21002189 | 21002338 | 21002189 | ID=cds.MS.gene066719.t1;Parent=MS.gene066719.t1 |
| Gene Sequence |
| Protein sequence |
