AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055603


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055603.t1 MTR_4g065050 71.034 145 42 0 26 170 10 154 6.93e-69 206
MS.gene055603.t1 MTR_4g065050 67.797 118 38 0 39 156 1 118 1.05e-52 164
MS.gene055603.t1 MTR_8g089030 52.747 91 39 3 17 104 36 125 1.30e-17 79.7
MS.gene055603.t1 MTR_8g089030 48.101 79 39 2 28 104 470 548 5.40e-11 60.5
MS.gene055603.t1 MTR_8g089030 52.747 91 39 3 17 104 36 125 1.53e-17 79.3
MS.gene055603.t1 MTR_8g089030 48.101 79 39 2 28 104 470 548 6.18e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGGTCCATTATGTAAAA+TGG 0.330791 4.3:+38906632 MS.gene055603:CDS
GGAAGAGGATATGTCTCTTC+AGG 0.339040 4.3:+38906494 MS.gene055603:CDS
GAACAACCGGAGCATCTTGC+AGG 0.385771 4.3:+38906615 MS.gene055603:CDS
TGCATTAATTGCGGAACAAC+CGG 0.397610 4.3:+38906602 MS.gene055603:CDS
GAAGAAACGGATCATATTCC+AGG 0.409810 4.3:+38906723 MS.gene055603:CDS
TGAATTGATTGCAGCCGAAT+TGG 0.427925 4.3:+38906473 MS.gene055603:CDS
TCTTCAGGATTCATTGAGAA+AGG 0.440370 4.3:+38906509 MS.gene055603:CDS
AGACATATCCTCTTCCAATT+CGG 0.468675 4.3:-38906487 None:intergenic
TGAATTTATTGAGGAAGAAA+CGG 0.471592 4.3:+38906710 MS.gene055603:CDS
CAATATTGATGAATTTATTG+AGG 0.493374 4.3:+38906701 MS.gene055603:CDS
TTCCGAGAAGTCCATGGAAA+AGG 0.505472 4.3:+38906231 MS.gene055603:CDS
AGAAGAGAATAATAAGCAAC+CGG 0.507208 4.3:+38906364 MS.gene055603:CDS
AATGGACCTGCAAGATGCTC+CGG 0.528367 4.3:-38906621 None:intergenic
ATTTGTCAATGAATTGTACA+TGG 0.532546 4.3:+38906791 MS.gene055603:CDS
GATTGCAGCCGAATTGGAAG+AGG 0.533785 4.3:+38906479 MS.gene055603:CDS
CAACATTTCCGAGAAGTCCA+TGG 0.547363 4.3:+38906225 MS.gene055603:CDS
GTCAATGAATTGTACATGGA+TGG 0.549535 4.3:+38906795 MS.gene055603:CDS
AAGGTATGCTGCTTTGTATG+TGG 0.584948 4.3:+38906824 MS.gene055603:CDS
TGTACATGGATGGACAAGAA+AGG 0.594997 4.3:+38906805 MS.gene055603:CDS
GTACATGAAAGCGAGAAAAG+AGG 0.599519 4.3:+38906665 MS.gene055603:CDS
GGAGATGGATGCATTAATTG+CGG 0.639286 4.3:+38906593 MS.gene055603:CDS
TTGTATGTGGAGAAGTCTAG+TGG 0.643539 4.3:+38906837 MS.gene055603:CDS
TGTATGTGGAGAAGTCTAGT+GGG 0.662396 4.3:+38906838 MS.gene055603:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACTGTAAGAAGTTAAA+AGG - chr4.3:38906907-38906926 None:intergenic 20.0%
!! AAAACTGTAAGAAGTTAAAA+GGG - chr4.3:38906906-38906925 None:intergenic 20.0%
!! CAATATTGATGAATTTATTG+AGG + chr4.3:38906701-38906720 MS.gene055603:CDS 20.0%
!!! AAACATTTTTTTTCAAAAGC+AGG - chr4.3:38906305-38906324 None:intergenic 20.0%
! AAAAAAAATGTTTCTTGCTG+TGG + chr4.3:38906312-38906331 MS.gene055603:intron 25.0%
! ATTTGTCAATGAATTGTACA+TGG + chr4.3:38906791-38906810 MS.gene055603:CDS 25.0%
! TGAATTTATTGAGGAAGAAA+CGG + chr4.3:38906710-38906729 MS.gene055603:CDS 25.0%
!! AAAAAAATGTTTCTTGCTGT+GGG + chr4.3:38906313-38906332 MS.gene055603:intron 25.0%
!! TTAGTGACCATTTTACATAA+TGG - chr4.3:38906642-38906661 None:intergenic 25.0%
AACTTGAATTTGGCTGTAAA+TGG + chr4.3:38906389-38906408 MS.gene055603:intron 30.0%
AGAAGAGAATAATAAGCAAC+CGG + chr4.3:38906364-38906383 MS.gene055603:CDS 30.0%
! ACAGTTTTTCTTGTCACTAT+AGG + chr4.3:38906918-38906937 MS.gene055603:intron 30.0%
!! TACTATTTTGAAAGAGGAGA+TGG + chr4.3:38906578-38906597 MS.gene055603:CDS 30.0%
!! TTACGCTACTATTTTGAAAG+AGG + chr4.3:38906572-38906591 MS.gene055603:CDS 30.0%
AAAAACCCTAAAAGAGAACG+AGG + chr4.3:38906548-38906567 MS.gene055603:CDS 35.0%
AGACATATCCTCTTCCAATT+CGG - chr4.3:38906490-38906509 None:intergenic 35.0%
GAATTTGGCTGTAAATGGAT+GGG + chr4.3:38906394-38906413 MS.gene055603:intron 35.0%
GTCAATGAATTGTACATGGA+TGG + chr4.3:38906795-38906814 MS.gene055603:CDS 35.0%
TCTTCAGGATTCATTGAGAA+AGG + chr4.3:38906509-38906528 MS.gene055603:CDS 35.0%
TGAATTTGGCTGTAAATGGA+TGG + chr4.3:38906393-38906412 MS.gene055603:intron 35.0%
TGCAGGTCCATTATGTAAAA+TGG + chr4.3:38906632-38906651 MS.gene055603:CDS 35.0%
TGCTTACTAGAAACTGTTTG+TGG + chr4.3:38906436-38906455 MS.gene055603:intron 35.0%
! ACAGCCAAATTCAAGTTTAC+CGG - chr4.3:38906386-38906405 None:intergenic 35.0%
! ATCTCTATTTTCAATGCACC+TGG - chr4.3:38906744-38906763 None:intergenic 35.0%
! GTAAACCTCGTTCTCTTTTA+GGG - chr4.3:38906556-38906575 None:intergenic 35.0%
GAAGAAACGGATCATATTCC+AGG + chr4.3:38906723-38906742 MS.gene055603:CDS 40.0%
GCAACCGGTAAACTTGAATT+TGG + chr4.3:38906379-38906398 MS.gene055603:intron 40.0%
GGAGATGGATGCATTAATTG+CGG + chr4.3:38906593-38906612 MS.gene055603:CDS 40.0%
GTACATGAAAGCGAGAAAAG+AGG + chr4.3:38906665-38906684 MS.gene055603:CDS 40.0%
TGAATTGATTGCAGCCGAAT+TGG + chr4.3:38906473-38906492 MS.gene055603:CDS 40.0%
TGCATTAATTGCGGAACAAC+CGG + chr4.3:38906602-38906621 MS.gene055603:CDS 40.0%
TGTACATGGATGGACAAGAA+AGG + chr4.3:38906805-38906824 MS.gene055603:CDS 40.0%
TGTATGTGGAGAAGTCTAGT+GGG + chr4.3:38906838-38906857 MS.gene055603:CDS 40.0%
TTGTATGTGGAGAAGTCTAG+TGG + chr4.3:38906837-38906856 MS.gene055603:CDS 40.0%
! CGTAAACCTCGTTCTCTTTT+AGG - chr4.3:38906557-38906576 None:intergenic 40.0%
! CTAGTGGGTAAGTTATACAC+TGG + chr4.3:38906853-38906872 MS.gene055603:intron 40.0%
!! AAGGTATGCTGCTTTGTATG+TGG + chr4.3:38906824-38906843 MS.gene055603:CDS 40.0%
CAACATTTCCGAGAAGTCCA+TGG + chr4.3:38906225-38906244 MS.gene055603:CDS 45.0%
GGAAGAGGATATGTCTCTTC+AGG + chr4.3:38906494-38906513 MS.gene055603:CDS 45.0%
TTCCGAGAAGTCCATGGAAA+AGG + chr4.3:38906231-38906250 MS.gene055603:CDS 45.0%
! CACCTTTTCCATGGACTTCT+CGG - chr4.3:38906236-38906255 None:intergenic 45.0%
! TGTATGTCGCACCTTTTCCA+TGG - chr4.3:38906245-38906264 None:intergenic 45.0%
AATGGACCTGCAAGATGCTC+CGG - chr4.3:38906624-38906643 None:intergenic 50.0%
GATTGCAGCCGAATTGGAAG+AGG + chr4.3:38906479-38906498 MS.gene055603:CDS 50.0%
! CTTGCTGTGGGCAAAACTGA+AGG + chr4.3:38906325-38906344 MS.gene055603:intron 50.0%
! GAACAACCGGAGCATCTTGC+AGG + chr4.3:38906615-38906634 MS.gene055603:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 38906217 38906982 38906217 ID=MS.gene055603
chr4.3 mRNA 38906217 38906982 38906217 ID=MS.gene055603.t1;Parent=MS.gene055603
chr4.3 exon 38906217 38906252 38906217 ID=MS.gene055603.t1.exon1;Parent=MS.gene055603.t1
chr4.3 CDS 38906217 38906252 38906217 ID=cds.MS.gene055603.t1;Parent=MS.gene055603.t1
chr4.3 exon 38906347 38906385 38906347 ID=MS.gene055603.t1.exon2;Parent=MS.gene055603.t1
chr4.3 CDS 38906347 38906385 38906347 ID=cds.MS.gene055603.t1;Parent=MS.gene055603.t1
chr4.3 exon 38906465 38906859 38906465 ID=MS.gene055603.t1.exon3;Parent=MS.gene055603.t1
chr4.3 CDS 38906465 38906859 38906465 ID=cds.MS.gene055603.t1;Parent=MS.gene055603.t1
chr4.3 exon 38906940 38906982 38906940 ID=MS.gene055603.t1.exon4;Parent=MS.gene055603.t1
chr4.3 CDS 38906940 38906982 38906940 ID=cds.MS.gene055603.t1;Parent=MS.gene055603.t1
Gene Sequence

>MS.gene055603

ATGAAGTTCAACATTTCCGAGAAGTCCATGGAAAAGGTGCGACATACATATATATGTTTTATTTATATGTTTAAGATCATTTGTACCTGCTTTTGAAAAAAAATGTTTCTTGCTGTGGGCAAAACTGAAGGAAAATGATAAATGTGTAGAAGAGAATAATAAGCAACCGGTAAACTTGAATTTGGCTGTAAATGGATGGGTTTTTCTTATTTTGTTAACTGCTTACTAGAAACTGTTTGTGGCTGCAGCAAATTGATGAATTGATTGCAGCCGAATTGGAAGAGGATATGTCTCTTCAGGATTCATTGAGAAAGGTTAATGACTTGTTTATAAAAACCCTAAAAGAGAACGAGGTTTACGCTACTATTTTGAAAGAGGAGATGGATGCATTAATTGCGGAACAACCGGAGCATCTTGCAGGTCCATTATGTAAAATGGTCACTAAAATGTACATGAAAGCGAGAAAAGAGGTCAGTGAATTGTACAATATTGATGAATTTATTGAGGAAGAAACGGATCATATTCCAGGTGCATTGAAAATAGAGATGATTAAATCGTACATGAAATTGCAAAAATTTGTCAATGAATTGTACATGGATGGACAAGAAAGGTATGCTGCTTTGTATGTGGAGAAGTCTAGTGGGTAAGTTATACACTGGATTTGTGTTGTGAGTAATTCTAGTTTTCCCTTTTAACTTCTTACAGTTTTTCTTGTCACTATAGGCTAATCAAAGACAAAACAGAAAAGCAAGACAGAAAGCCATAG

Protein sequence

>MS.gene055603.t1

MKFNISEKSMEKENDKCVEENNKQPQIDELIAAELEEDMSLQDSLRKVNDLFIKTLKENEVYATILKEEMDALIAEQPEHLAGPLCKMVTKMYMKARKEVSELYNIDEFIEEETDHIPGALKIEMIKSYMKLQKFVNELYMDGQERYAALYVEKSSGLIKDKTEKQDRKP