AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066826

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066826.t1 MTR_3g085570 97.849 372 8 0 1 372 1 372 0.0 759
MS.gene066826.t1 MTR_8g080370 75.200 375 88 2 1 372 16 388 0.0 589
MS.gene066826.t1 MTR_4g114110 30.634 284 177 9 30 304 76 348 2.32e-35 134
MS.gene066826.t1 MTR_8g085600 29.242 277 180 8 34 303 76 343 1.07e-29 119
MS.gene066826.t1 MTR_8g030940 27.946 297 172 10 31 306 21 296 1.11e-27 112
MS.gene066826.t1 MTR_7g095220 31.395 258 155 7 33 277 2 250 6.16e-27 109
MS.gene066826.t1 MTR_0105s0130 25.649 308 203 6 39 330 93 390 4.11e-24 103
MS.gene066826.t1 MTR_2g094093 25.305 328 218 8 30 344 15 328 2.05e-23 100
MS.gene066826.t1 MTR_4g108280 23.988 321 211 7 31 330 27 335 2.90e-23 100
MS.gene066826.t1 MTR_2g019230 27.044 318 211 8 3 309 27 334 4.10e-23 100
MS.gene066826.t1 MTR_4g022400 24.854 342 223 9 9 330 41 368 6.31e-23 99.8
MS.gene066826.t1 MTR_0345s0010 25.325 308 204 6 39 330 93 390 1.76e-22 98.6
MS.gene066826.t1 MTR_1330s0010 25.325 308 204 6 39 330 106 403 2.29e-22 98.2
MS.gene066826.t1 MTR_0105s0170 25.000 316 211 6 31 330 98 403 4.47e-22 97.4
MS.gene066826.t1 MTR_0105s0040 25.000 316 211 6 31 330 183 488 6.72e-22 97.4
MS.gene066826.t1 MTR_4g022260 25.000 308 204 7 39 330 75 371 1.03e-21 96.3
MS.gene066826.t1 MTR_0496s0010 25.649 308 203 7 39 330 75 372 1.07e-21 95.9
MS.gene066826.t1 MTR_0105s0020 25.159 314 197 8 39 330 93 390 1.44e-21 95.9
MS.gene066826.t1 MTR_5g039390 28.163 245 151 7 31 267 125 352 8.87e-21 94.0
MS.gene066826.t1 MTR_2g008440 24.798 371 205 12 30 327 38 407 5.50e-20 91.7
MS.gene066826.t1 MTR_8g468030 27.306 271 168 8 5 267 124 373 6.40e-19 88.6
MS.gene066826.t1 MTR_6g023805 24.615 325 196 12 36 330 57 362 7.47e-18 84.7
MS.gene066826.t1 MTR_3g073640 24.207 347 221 12 28 344 36 370 2.01e-17 84.0
MS.gene066826.t1 MTR_3g108860 26.064 188 119 4 29 213 11 181 4.70e-14 72.8
MS.gene066826.t1 MTR_8g085650 24.242 231 153 4 35 247 7 233 1.36e-13 71.6
MS.gene066826.t1 MTR_7g029290 26.638 229 156 4 16 241 36 255 2.60e-13 71.2
MS.gene066826.t1 MTR_2g012790 36.082 97 59 2 146 240 103 198 1.02e-12 68.9
MS.gene066826.t1 MTR_2g102370 26.606 218 125 6 35 226 3 211 2.68e-11 64.7
MS.gene066826.t1 MTR_7g070320 25.714 245 165 9 72 305 412 650 4.17e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066826.t1 AT1G67480 62.757 341 127 0 32 372 36 376 1.40e-164 465
MS.gene066826.t1 AT1G67480 62.757 341 127 0 32 372 36 376 1.40e-164 465
MS.gene066826.t1 AT1G67480 62.757 341 127 0 32 372 36 376 1.40e-164 465
MS.gene066826.t1 AT1G27420 40.169 356 188 10 27 370 2 344 2.71e-86 265
MS.gene066826.t1 AT1G55270 29.643 280 177 9 34 304 77 345 2.11e-32 126
MS.gene066826.t1 AT5G60570 26.625 323 210 8 35 344 49 357 2.52e-28 114
MS.gene066826.t1 AT5G60570 26.625 323 210 8 35 344 49 357 2.52e-28 114
MS.gene066826.t1 AT5G60570 26.625 323 210 8 35 344 49 357 2.52e-28 114
MS.gene066826.t1 AT1G16250 25.896 251 170 5 28 267 1 246 1.39e-26 109
MS.gene066826.t1 AT1G16250 25.896 251 170 5 28 267 1 246 1.39e-26 109
MS.gene066826.t1 AT3G63220 28.571 308 192 9 33 329 9 299 9.36e-25 103
MS.gene066826.t1 AT3G63220 28.571 308 192 9 33 329 9 299 9.36e-25 103
MS.gene066826.t1 AT3G63220 28.431 306 195 8 33 329 2 292 1.11e-24 103
MS.gene066826.t1 AT2G24540 23.620 326 190 7 5 306 11 301 5.04e-22 96.7
MS.gene066826.t1 AT3G61350 34.078 179 112 4 35 210 21 196 6.61e-22 95.9
MS.gene066826.t1 AT3G61350 34.078 179 112 4 35 210 21 196 6.61e-22 95.9
MS.gene066826.t1 AT1G30090 26.207 290 191 7 34 309 52 332 2.56e-21 94.7
MS.gene066826.t1 AT1G74510 28.070 228 141 6 47 267 107 318 1.16e-20 93.6
MS.gene066826.t1 AT1G74510 28.070 228 141 6 47 267 107 318 1.16e-20 93.6
MS.gene066826.t1 AT1G74510 28.070 228 141 6 47 267 107 318 1.16e-20 93.6
MS.gene066826.t1 AT1G55270 27.193 228 146 9 86 304 3 219 1.31e-19 88.6
MS.gene066826.t1 AT1G26930 27.143 210 144 2 31 240 67 267 3.00e-19 89.0
MS.gene066826.t1 AT3G27150 24.501 351 225 11 9 337 45 377 4.78e-19 88.6
MS.gene066826.t1 AT3G27150 24.501 351 225 11 9 337 45 377 4.78e-19 88.6
MS.gene066826.t1 AT1G22040 25.312 320 174 8 8 268 16 329 1.56e-18 87.4
MS.gene066826.t1 AT5G26960 26.277 274 157 9 31 277 39 294 1.85e-18 86.7
MS.gene066826.t1 AT2G02870 24.380 242 165 4 31 267 113 341 3.70e-17 83.2
MS.gene066826.t1 AT2G02870 24.380 242 165 4 31 267 113 341 3.70e-17 83.2
MS.gene066826.t1 AT2G02870 24.380 242 165 4 31 267 113 341 3.70e-17 83.2
MS.gene066826.t1 AT1G26930 28.934 197 123 4 31 223 67 250 4.31e-17 82.4
MS.gene066826.t1 AT1G80440 27.572 243 159 7 35 262 3 243 5.62e-17 81.6
MS.gene066826.t1 AT2G21950 25.347 288 186 8 30 303 15 287 7.96e-17 81.6
MS.gene066826.t1 AT1G14330 23.881 268 173 7 7 265 71 316 9.11e-17 81.6
MS.gene066826.t1 AT2G29830 28.718 195 126 4 29 218 23 209 4.11e-16 79.3
MS.gene066826.t1 AT4G03030 29.630 216 125 7 35 226 38 250 3.03e-15 77.0
MS.gene066826.t1 AT2G44130 26.103 272 157 8 35 267 19 285 3.51e-15 77.0
MS.gene066826.t1 AT3G46050 30.556 180 112 7 39 213 21 192 9.61e-15 75.1
MS.gene066826.t1 AT4G33900 27.979 193 124 5 39 217 15 206 1.49e-14 74.7
MS.gene066826.t1 AT1G15670 27.092 251 154 8 35 262 3 247 1.78e-13 71.2
MS.gene066826.t1 AT3G43710 26.570 207 140 5 39 237 29 231 1.39e-12 68.9
MS.gene066826.t1 AT4G39290 28.495 186 122 6 39 217 16 197 1.56e-12 68.6
MS.gene066826.t1 AT1G19460 22.222 333 211 12 5 314 24 331 1.99e-12 68.6
MS.gene066826.t1 AT2G29860 25.269 186 121 5 39 218 23 196 2.53e-12 66.6
MS.gene066826.t1 AT2G29600 26.244 221 137 9 35 240 57 266 4.73e-12 67.4
MS.gene066826.t1 AT5G03000 27.000 200 129 7 26 216 28 219 1.46e-11 65.5
MS.gene066826.t1 AT5G02980 27.869 183 120 4 33 213 11 183 1.64e-11 65.1
MS.gene066826.t1 AT1G19470 21.148 331 215 11 5 314 24 329 1.82e-11 65.5
MS.gene066826.t1 AT5G40680 24.779 339 214 14 17 330 43 365 6.77e-11 63.9

Find 122 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGAAAGCTGTTGCTATTTC+CGG 0.181747 3.4:+79903566 MS.gene066826:CDS
AAATATGTGTTTCACCAATT+TGG 0.248722 3.4:+79902413 None:intergenic
TATAACAATGTTTGATTAGT+AGG 0.249213 3.4:-79903600 None:intergenic
AATATGATTCTTACCTTAAC+AGG 0.252725 3.4:+79902886 MS.gene066826:CDS
TATGATCCAAACGCGGCTTC+GGG 0.274671 3.4:+79903621 MS.gene066826:CDS
AACGGCTTGGTTTATATTGC+TGG 0.291844 3.4:+79903144 MS.gene066826:CDS
GTTTATATTGCTGGAGGCTA+TGG 0.301729 3.4:+79903153 MS.gene066826:CDS
TCGCACCAGCTGTGCTTCTC+AGG 0.305793 3.4:-79903363 None:intergenic
TGTTCCCCGTTCCAACTTCC+CGG 0.306851 3.4:+79902566 MS.gene066826:CDS
ATATGATCCAAACGCGGCTT+CGG 0.322371 3.4:+79903620 MS.gene066826:CDS
TTTATCTTGATTGACCAAAT+TGG 0.328883 3.4:-79902427 None:intergenic
AGAAATGGAGGTTGTTTATT+CGG 0.328904 3.4:+79902607 MS.gene066826:CDS
TTGTTTAACCGTTGAGGCTC+TGG 0.356565 3.4:+79902479 MS.gene066826:CDS
CCGTTCCAACTTCCCGGCCA+TGG 0.360625 3.4:+79902572 MS.gene066826:CDS
GGTCCAGCAAAGGCTTCGTT+CGG 0.367698 3.4:+79902777 MS.gene066826:CDS
GTCCAGCAAAGGCTTCGTTC+GGG 0.376162 3.4:+79902778 MS.gene066826:CDS
AAAGGCTTCGTTCGGGGTTG+TGG 0.383631 3.4:+79902785 MS.gene066826:CDS
AGTGTCCACTTGTCAGTATC+TGG 0.384601 3.4:-79903216 None:intergenic
TCTGACCCCGAAGCCGCGTT+TGG 0.384699 3.4:-79903627 None:intergenic
GAAGTTAATTATACCTGTTA+AGG 0.389829 3.4:-79902899 None:intergenic
ACCCCGAACGAAGCCTTTGC+TGG 0.393719 3.4:-79902780 None:intergenic
CATGGCCGGGAAGTTGGAAC+GGG 0.404354 3.4:-79902571 None:intergenic
TTTATATTGCTGGAGGCTAT+GGG 0.405335 3.4:+79903154 MS.gene066826:CDS
ATTTGTGATGGTGCGAAAAT+CGG 0.408114 3.4:+79902638 MS.gene066826:CDS
TTAGTAGGTTCTTTCTCAAC+CGG 0.410021 3.4:-79903585 None:intergenic
CAATACTTTGCAACATCATC+AGG 0.414835 3.4:-79902534 None:intergenic
TTCGGGGTTGTGGTTCTTAA+CGG 0.416406 3.4:+79902795 MS.gene066826:CDS
TCAGGAAGCCCAGGTAGAAT+AGG 0.416413 3.4:-79902516 None:intergenic
ACCACCCATGGCCGGGAAGT+TGG 0.417943 3.4:-79902577 None:intergenic
ACACTTGAGCTCCCTGTCAA+TGG 0.420117 3.4:-79903519 None:intergenic
TAACGGAAAGCTTCTTATCA+TGG 0.427536 3.4:+79902812 MS.gene066826:CDS
CACTTGAGCTCCCTGTCAAT+GGG 0.435672 3.4:-79903518 None:intergenic
CAGGAAGCCCAGGTAGAATA+GGG 0.444377 3.4:-79902515 None:intergenic
CGTTCCAACTTCCCGGCCAT+GGG 0.444701 3.4:+79902573 MS.gene066826:CDS
GATTAAGAATGGCTGTGTTA+TGG 0.449162 3.4:+79903386 MS.gene066826:CDS
GGAAGGTCAAGCTTCACTAT+TGG 0.453050 3.4:+79903312 MS.gene066826:CDS
AACGAAGCCTTTGCTGGACC+AGG 0.455629 3.4:-79902774 None:intergenic
TGTTAAGGTAAGAATCATAT+TGG 0.455737 3.4:-79902884 None:intergenic
TTGCTGACACCACCCATGGC+CGG 0.456190 3.4:-79902585 None:intergenic
ATGGTACCCGTCCCATTGAC+AGG 0.457096 3.4:+79903507 MS.gene066826:CDS
GCCTTTGCTGGACCAGGCAT+TGG 0.462939 3.4:-79902768 None:intergenic
AAGCAAAGCAACCCCACCTT+GGG 0.472401 3.4:-79903257 None:intergenic
GAGCCTTCGTCGCCCAAGGT+GGG 0.473693 3.4:+79903245 MS.gene066826:CDS
GGTCACAGCTCATGCAGTAT+TGG 0.477022 3.4:+79903407 MS.gene066826:CDS
ACTTCAGACATAAGGCCATC+TGG 0.481593 3.4:+79903657 MS.gene066826:CDS
TGGGTGGTGTCAGCAAGAAA+TGG 0.482075 3.4:+79902592 MS.gene066826:CDS
TGCTGACACCACCCATGGCC+GGG 0.483995 3.4:-79902584 None:intergenic
GTGATGGTGCGAAAATCGGC+AGG 0.486945 3.4:+79902642 MS.gene066826:CDS
TGAAGGGAGGGAAAGCCATT+GGG 0.487056 3.4:+79902704 MS.gene066826:CDS
GGAAAGCCATTGGGAGGTCA+TGG 0.488773 3.4:+79902713 MS.gene066826:CDS
TTTGCTGGACCAGGCATTGG+TGG 0.494551 3.4:-79902765 None:intergenic
CTGGACCAGGCATTGGTGGA+AGG 0.496890 3.4:-79902761 None:intergenic
CAAGCAAAGCAACCCCACCT+TGG 0.497340 3.4:-79903258 None:intergenic
TGCTTAACGTTGGACAGTGA+AGG 0.500086 3.4:+79902687 MS.gene066826:CDS
GTGAAGGGAGGGAAAGCCAT+TGG 0.501961 3.4:+79902703 MS.gene066826:CDS
GCTGGCTATTCAGCTATTGA+AGG 0.502519 3.4:+79902834 MS.gene066826:CDS
GCTGGAGGCTATGGGGTGAA+TGG 0.504433 3.4:+79903162 MS.gene066826:CDS
CCATGGCCGGGAAGTTGGAA+CGG 0.504809 3.4:-79902572 None:intergenic
CGATCCCTTCCACCAATGCC+TGG 0.510965 3.4:+79902756 MS.gene066826:CDS
AGTGGCAAACTTCAGACATA+AGG 0.511223 3.4:+79903649 MS.gene066826:CDS
GCTTAACGTTGGACAGTGAA+GGG 0.511344 3.4:+79902688 MS.gene066826:CDS
GGAAAGCTTCTTATCATGGC+TGG 0.517692 3.4:+79902816 MS.gene066826:CDS
GTTATATATATCAACAAACT+TGG 0.518890 3.4:-79903340 None:intergenic
TCCAACTTCCCGGCCATGGG+TGG 0.522273 3.4:+79902576 MS.gene066826:CDS
AGCCTTCGTCGCCCAAGGTG+GGG 0.526676 3.4:+79903246 MS.gene066826:CDS
TGAAGATAAGCTCTATGTCA+TGG 0.531034 3.4:+79903287 MS.gene066826:CDS
GATGATAGCCCTATTCTACC+TGG 0.534891 3.4:+79902507 MS.gene066826:CDS
AGGTGGGGTTGCTTTGCTTG+TGG 0.538747 3.4:+79903261 MS.gene066826:CDS
ATAACCCTGAGAAGCACAGC+TGG 0.539110 3.4:+79903358 MS.gene066826:CDS
AACCCCACCTTGGGCGACGA+AGG 0.539939 3.4:-79903248 None:intergenic
ATCTTTATCCAGAGCCTCAA+CGG 0.544136 3.4:-79902487 None:intergenic
TCAGTCCTGAAAACAACTCA+TGG 0.545566 3.4:+79903481 MS.gene066826:CDS
GAGCTCCCTGTCAATGGGAC+GGG 0.546022 3.4:-79903513 None:intergenic
AGCTCTATGTCATGGGTGGA+AGG 0.548574 3.4:+79903295 MS.gene066826:CDS
CGCACCAGCTGTGCTTCTCA+GGG 0.550037 3.4:-79903362 None:intergenic
AGCTGGTGCGAGATTAAGAA+TGG 0.554469 3.4:+79903375 MS.gene066826:CDS
TTTCTTGCTGACACCACCCA+TGG 0.555041 3.4:-79902589 None:intergenic
TGGACCAGGCATTGGTGGAA+GGG 0.555255 3.4:-79902760 None:intergenic
TTTCGATTCGGGATACTGGA+CGG 0.557225 3.4:+79903546 MS.gene066826:CDS
CGGCAGGGTTGCTTGAGGAA+TGG 0.560230 3.4:+79902658 MS.gene066826:CDS
TGGGAGGTCATGGATAGTCT+TGG 0.562088 3.4:+79902723 MS.gene066826:CDS
CAATCATTGTTTAACCGTTG+AGG 0.563118 3.4:+79902473 MS.gene066826:CDS
GCAACATCATCAGGAAGCCC+AGG 0.567649 3.4:-79902525 None:intergenic
TTGTGCCGAAGTGAACGGCT+TGG 0.571035 3.4:+79903131 MS.gene066826:CDS
GAAGATAAGCTCTATGTCAT+GGG 0.576973 3.4:+79903288 MS.gene066826:CDS
AGAGCCTTCGTCGCCCAAGG+TGG 0.577508 3.4:+79903244 MS.gene066826:CDS
ACCAATGCCTGGTCCAGCAA+AGG 0.578481 3.4:+79902767 MS.gene066826:CDS
GTAAACCTCATCTGAGGCAA+CGG 0.585230 3.4:-79902862 None:intergenic
AACCGCCGTTGCCTCAGATG+AGG 0.590834 3.4:+79902857 MS.gene066826:CDS
TAGAGAGCCTTCGTCGCCCA+AGG 0.593325 3.4:+79903241 MS.gene066826:CDS
TTCGCTTGTGCCGAAGTGAA+CGG 0.596628 3.4:+79903126 MS.gene066826:CDS
TGAGCTCCCTGTCAATGGGA+CGG 0.598268 3.4:-79903514 None:intergenic
GGCTTGGTTTATATTGCTGG+AGG 0.598885 3.4:+79903147 MS.gene066826:CDS
ATGGCTCTATTGCTTAACGT+TGG 0.599337 3.4:+79902677 MS.gene066826:CDS
TTATATTGCTGGAGGCTATG+GGG 0.603244 3.4:+79903155 MS.gene066826:CDS
ATGATCCAAACGCGGCTTCG+GGG 0.605908 3.4:+79903622 MS.gene066826:CDS
AACGTTGGACAGTGAAGGGA+GGG 0.606186 3.4:+79902692 MS.gene066826:CDS
TCGGAGCAAAGAATTTGTGA+TGG 0.607451 3.4:+79902626 MS.gene066826:CDS
TTGACAGGGAGCTCAAGTGT+TGG 0.612609 3.4:+79903522 MS.gene066826:CDS
TCCAGCAAAGGCTTCGTTCG+GGG 0.616864 3.4:+79902779 MS.gene066826:CDS
ATGATAGCCCTATTCTACCT+GGG 0.619633 3.4:+79902508 MS.gene066826:CDS
CAGATTATCAAACATGAATG+TGG 0.621881 3.4:+79903092 MS.gene066826:CDS
GACTATCCATGACCTCCCAA+TGG 0.625423 3.4:-79902719 None:intergenic
GATAAGCTCTATGTCATGGG+TGG 0.626294 3.4:+79903291 MS.gene066826:CDS
CTTGTGCAGTGTAACAATGA+AGG 0.630215 3.4:+79903686 MS.gene066826:CDS
CTGCACAAGCAGAAACCAGA+TGG 0.632831 3.4:-79903672 None:intergenic
AAAATCGGCAGGGTTGCTTG+AGG 0.636803 3.4:+79902653 MS.gene066826:CDS
TGAAAACAACTCATGGACAA+TGG 0.637316 3.4:+79903488 MS.gene066826:CDS
TGGATCGTACATCTCAACAC+TGG 0.639843 3.4:-79903196 None:intergenic
ACGCGGCTTCGGGGTCAGAG+TGG 0.648594 3.4:+79903631 MS.gene066826:CDS
ACGATCCAGATACTGACAAG+TGG 0.649132 3.4:+79903211 MS.gene066826:CDS
TAACGTTGGACAGTGAAGGG+AGG 0.653317 3.4:+79902691 MS.gene066826:CDS
ATAAACCAAGCCGTTCACTT+CGG 0.657321 3.4:-79903136 None:intergenic
AGGGAGGGAAAGCCATTGGG+AGG 0.658191 3.4:+79902707 MS.gene066826:CDS
TGGTACCCGTCCCATTGACA+GGG 0.662034 3.4:+79903508 MS.gene066826:CDS
AACCTCATCTGAGGCAACGG+CGG 0.663566 3.4:-79902859 None:intergenic
TGGAACGGGGAACAAGTGCA+AGG 0.676362 3.4:-79902557 None:intergenic
ATGGCCGGGAAGTTGGAACG+GGG 0.676587 3.4:-79902570 None:intergenic
ATATTGGTAAACCTCATCTG+AGG 0.683760 3.4:-79902868 None:intergenic
TGATGGTGCGAAAATCGGCA+GGG 0.721787 3.4:+79902643 MS.gene066826:CDS
GTGGTGTCAGCAAGAAATGG+AGG 0.732895 3.4:+79902595 MS.gene066826:CDS
ATTGTTATATGATCCAAACG+CGG 0.751620 3.4:+79903614 MS.gene066826:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTTTCAAAAATTATGAAA+TGG - chr3.4:79903020-79903039 None:intergenic 15.0%
!! AAAATTATGAAATGGATGAT+AGG - chr3.4:79903012-79903031 None:intergenic 20.0%
!! GTTATATATATCAACAAACT+TGG - chr3.4:79903343-79903362 None:intergenic 20.0%
!! TATAACAATGTTTGATTAGT+AGG - chr3.4:79903603-79903622 None:intergenic 20.0%
! AATATGATTCTTACCTTAAC+AGG + chr3.4:79902886-79902905 MS.gene066826:CDS 25.0%
! GAAGTTAATTATACCTGTTA+AGG - chr3.4:79902902-79902921 None:intergenic 25.0%
! TACCATCAATTAAATTACGA+CGG - chr3.4:79902987-79903006 None:intergenic 25.0%
! TGTTAAGGTAAGAATCATAT+TGG - chr3.4:79902887-79902906 None:intergenic 25.0%
! TTTATCTTGATTGACCAAAT+TGG - chr3.4:79902430-79902449 None:intergenic 25.0%
AGAAATGGAGGTTGTTTATT+CGG + chr3.4:79902607-79902626 MS.gene066826:CDS 30.0%
ATTGTTATATGATCCAAACG+CGG + chr3.4:79903614-79903633 MS.gene066826:CDS 30.0%
CAGATTATCAAACATGAATG+TGG + chr3.4:79903092-79903111 MS.gene066826:CDS 30.0%
TTCTCAATTGCAATTGTAGT+TGG + chr3.4:79903067-79903086 MS.gene066826:intron 30.0%
!! AGAAGTTGTTTTGTATTGAG+TGG + chr3.4:79903433-79903452 MS.gene066826:CDS 30.0%
ATATTGGTAAACCTCATCTG+AGG - chr3.4:79902871-79902890 None:intergenic 35.0%
CAATACTTTGCAACATCATC+AGG - chr3.4:79902537-79902556 None:intergenic 35.0%
CAATCATTGTTTAACCGTTG+AGG + chr3.4:79902473-79902492 MS.gene066826:CDS 35.0%
CTCCGTCGTAATTTAATTGA+TGG + chr3.4:79902982-79903001 MS.gene066826:intron 35.0%
GAAGATAAGCTCTATGTCAT+GGG + chr3.4:79903288-79903307 MS.gene066826:CDS 35.0%
GATTAAGAATGGCTGTGTTA+TGG + chr3.4:79903386-79903405 MS.gene066826:CDS 35.0%
GTATTGAGTGGAAAAATCAG+CGG + chr3.4:79903445-79903464 MS.gene066826:CDS 35.0%
TAACGGAAAGCTTCTTATCA+TGG + chr3.4:79902812-79902831 MS.gene066826:CDS 35.0%
TGAAAACAACTCATGGACAA+TGG + chr3.4:79903488-79903507 MS.gene066826:CDS 35.0%
TGAAGATAAGCTCTATGTCA+TGG + chr3.4:79903287-79903306 MS.gene066826:CDS 35.0%
TTAGTAGGTTCTTTCTCAAC+CGG - chr3.4:79903588-79903607 None:intergenic 35.0%
TTTATATTGCTGGAGGCTAT+GGG + chr3.4:79903154-79903173 MS.gene066826:CDS 35.0%
! ATTTGTGATGGTGCGAAAAT+CGG + chr3.4:79902638-79902657 MS.gene066826:CDS 35.0%
!! ATTGTCCATGAGTTGTTTTC+AGG - chr3.4:79903489-79903508 None:intergenic 35.0%
!!! TCAAGTGTTGGTTTTCGATT+CGG + chr3.4:79903534-79903553 MS.gene066826:CDS 35.0%
AGTGGCAAACTTCAGACATA+AGG + chr3.4:79903649-79903668 MS.gene066826:CDS 40.0%
ATAAACCAAGCCGTTCACTT+CGG - chr3.4:79903139-79903158 None:intergenic 40.0%
ATCTTTATCCAGAGCCTCAA+CGG - chr3.4:79902490-79902509 None:intergenic 40.0%
ATGATAGCCCTATTCTACCT+GGG + chr3.4:79902508-79902527 MS.gene066826:CDS 40.0%
CTTGTGCAGTGTAACAATGA+AGG + chr3.4:79903686-79903705 MS.gene066826:CDS 40.0%
GTTTATATTGCTGGAGGCTA+TGG + chr3.4:79903153-79903172 MS.gene066826:CDS 40.0%
TCAGTCCTGAAAACAACTCA+TGG + chr3.4:79903481-79903500 MS.gene066826:CDS 40.0%
TCGGAGCAAAGAATTTGTGA+TGG + chr3.4:79902626-79902645 MS.gene066826:CDS 40.0%
TTATATTGCTGGAGGCTATG+GGG + chr3.4:79903155-79903174 MS.gene066826:CDS 40.0%
! ATGGCTCTATTGCTTAACGT+TGG + chr3.4:79902677-79902696 MS.gene066826:CDS 40.0%
!! AACGGCTTGGTTTATATTGC+TGG + chr3.4:79903144-79903163 MS.gene066826:CDS 40.0%
!!! CAAGTGTTGGTTTTCGATTC+GGG + chr3.4:79903535-79903554 MS.gene066826:CDS 40.0%
ACGATCCAGATACTGACAAG+TGG + chr3.4:79903211-79903230 MS.gene066826:CDS 45.0%
ACTTCAGACATAAGGCCATC+TGG + chr3.4:79903657-79903676 MS.gene066826:CDS 45.0%
AGCTGGTGCGAGATTAAGAA+TGG + chr3.4:79903375-79903394 MS.gene066826:CDS 45.0%
AGTGTCCACTTGTCAGTATC+TGG - chr3.4:79903219-79903238 None:intergenic 45.0%
ATATGATCCAAACGCGGCTT+CGG + chr3.4:79903620-79903639 MS.gene066826:CDS 45.0%
GATAAGCTCTATGTCATGGG+TGG + chr3.4:79903291-79903310 MS.gene066826:CDS 45.0%
GATGATAGCCCTATTCTACC+TGG + chr3.4:79902507-79902526 MS.gene066826:CDS 45.0%
GCTGGCTATTCAGCTATTGA+AGG + chr3.4:79902834-79902853 MS.gene066826:CDS 45.0%
GCTTAACGTTGGACAGTGAA+GGG + chr3.4:79902688-79902707 MS.gene066826:CDS 45.0%
GGAAAGCTTCTTATCATGGC+TGG + chr3.4:79902816-79902835 MS.gene066826:CDS 45.0%
GGAAGGTCAAGCTTCACTAT+TGG + chr3.4:79903312-79903331 MS.gene066826:CDS 45.0%
TGCTTAACGTTGGACAGTGA+AGG + chr3.4:79902687-79902706 MS.gene066826:CDS 45.0%
TGGATCGTACATCTCAACAC+TGG - chr3.4:79903199-79903218 None:intergenic 45.0%
TTCGGGGTTGTGGTTCTTAA+CGG + chr3.4:79902795-79902814 MS.gene066826:CDS 45.0%
TTGTTTAACCGTTGAGGCTC+TGG + chr3.4:79902479-79902498 MS.gene066826:CDS 45.0%
! GGCTTGGTTTATATTGCTGG+AGG + chr3.4:79903147-79903166 MS.gene066826:CDS 45.0%
! GTAAACCTCATCTGAGGCAA+CGG - chr3.4:79902865-79902884 None:intergenic 45.0%
!! CGGAAAGCTGTTGCTATTTC+CGG + chr3.4:79903566-79903585 MS.gene066826:CDS 45.0%
!! TTTCGATTCGGGATACTGGA+CGG + chr3.4:79903546-79903565 MS.gene066826:CDS 45.0%
!!! TGGTTTTCGATTCGGGATAC+TGG + chr3.4:79903542-79903561 MS.gene066826:CDS 45.0%
AAAATCGGCAGGGTTGCTTG+AGG + chr3.4:79902653-79902672 MS.gene066826:CDS 50.0%
AACGTTGGACAGTGAAGGGA+GGG + chr3.4:79902692-79902711 MS.gene066826:CDS 50.0%
AAGCAAAGCAACCCCACCTT+GGG - chr3.4:79903260-79903279 None:intergenic 50.0%
ACACTTGAGCTCCCTGTCAA+TGG - chr3.4:79903522-79903541 None:intergenic 50.0%
AGCTCTATGTCATGGGTGGA+AGG + chr3.4:79903295-79903314 MS.gene066826:CDS 50.0%
CACTTGAGCTCCCTGTCAAT+GGG - chr3.4:79903521-79903540 None:intergenic 50.0%
CAGGAAGCCCAGGTAGAATA+GGG - chr3.4:79902518-79902537 None:intergenic 50.0%
CTGCACAAGCAGAAACCAGA+TGG - chr3.4:79903675-79903694 None:intergenic 50.0%
GACTATCCATGACCTCCCAA+TGG - chr3.4:79902722-79902741 None:intergenic 50.0%
GGTCACAGCTCATGCAGTAT+TGG + chr3.4:79903407-79903426 MS.gene066826:CDS 50.0%
TAACGTTGGACAGTGAAGGG+AGG + chr3.4:79902691-79902710 MS.gene066826:CDS 50.0%
TATGATCCAAACGCGGCTTC+GGG + chr3.4:79903621-79903640 MS.gene066826:CDS 50.0%
TCAGGAAGCCCAGGTAGAAT+AGG - chr3.4:79902519-79902538 None:intergenic 50.0%
TGAAGGGAGGGAAAGCCATT+GGG + chr3.4:79902704-79902723 MS.gene066826:CDS 50.0%
TGGGAGGTCATGGATAGTCT+TGG + chr3.4:79902723-79902742 MS.gene066826:CDS 50.0%
TTCGCTTGTGCCGAAGTGAA+CGG + chr3.4:79903126-79903145 MS.gene066826:CDS 50.0%
TTGACAGGGAGCTCAAGTGT+TGG + chr3.4:79903522-79903541 MS.gene066826:CDS 50.0%
TTTCTTGCTGACACCACCCA+TGG - chr3.4:79902592-79902611 None:intergenic 50.0%
! ATAACCCTGAGAAGCACAGC+TGG + chr3.4:79903358-79903377 MS.gene066826:CDS 50.0%
! GTGGTGTCAGCAAGAAATGG+AGG + chr3.4:79902595-79902614 MS.gene066826:CDS 50.0%
! TGATGGTGCGAAAATCGGCA+GGG + chr3.4:79902643-79902662 MS.gene066826:CDS 50.0%
! TGGGTGGTGTCAGCAAGAAA+TGG + chr3.4:79902592-79902611 MS.gene066826:CDS 50.0%
AAAGGCTTCGTTCGGGGTTG+TGG + chr3.4:79902785-79902804 MS.gene066826:CDS 55.0%
AACGAAGCCTTTGCTGGACC+AGG - chr3.4:79902777-79902796 None:intergenic 55.0%
ACCAATGCCTGGTCCAGCAA+AGG + chr3.4:79902767-79902786 MS.gene066826:CDS 55.0%
ATGATCCAAACGCGGCTTCG+GGG + chr3.4:79903622-79903641 MS.gene066826:CDS 55.0%
ATGGTACCCGTCCCATTGAC+AGG + chr3.4:79903507-79903526 MS.gene066826:CDS 55.0%
CAAGCAAAGCAACCCCACCT+TGG - chr3.4:79903261-79903280 None:intergenic 55.0%
GCAACATCATCAGGAAGCCC+AGG - chr3.4:79902528-79902547 None:intergenic 55.0%
GGAAAGCCATTGGGAGGTCA+TGG + chr3.4:79902713-79902732 MS.gene066826:CDS 55.0%
GGTCCAGCAAAGGCTTCGTT+CGG + chr3.4:79902777-79902796 MS.gene066826:CDS 55.0%
GTCCAGCAAAGGCTTCGTTC+GGG + chr3.4:79902778-79902797 MS.gene066826:CDS 55.0%
GTGAAGGGAGGGAAAGCCAT+TGG + chr3.4:79902703-79902722 MS.gene066826:CDS 55.0%
TCCAGCAAAGGCTTCGTTCG+GGG + chr3.4:79902779-79902798 MS.gene066826:CDS 55.0%
TGAGCTCCCTGTCAATGGGA+CGG - chr3.4:79903517-79903536 None:intergenic 55.0%
TGGAACGGGGAACAAGTGCA+AGG - chr3.4:79902560-79902579 None:intergenic 55.0%
TGGTACCCGTCCCATTGACA+GGG + chr3.4:79903508-79903527 MS.gene066826:CDS 55.0%
TGTTCCCCGTTCCAACTTCC+CGG + chr3.4:79902566-79902585 MS.gene066826:CDS 55.0%
TTGTGCCGAAGTGAACGGCT+TGG + chr3.4:79903131-79903150 MS.gene066826:CDS 55.0%
! AACCTCATCTGAGGCAACGG+CGG - chr3.4:79902862-79902881 None:intergenic 55.0%
! AGGTGGGGTTGCTTTGCTTG+TGG + chr3.4:79903261-79903280 MS.gene066826:CDS 55.0%
! GTGATGGTGCGAAAATCGGC+AGG + chr3.4:79902642-79902661 MS.gene066826:CDS 55.0%
!! TGGACCAGGCATTGGTGGAA+GGG - chr3.4:79902763-79902782 None:intergenic 55.0%
!! TTTGCTGGACCAGGCATTGG+TGG - chr3.4:79902768-79902787 None:intergenic 55.0%
AACCGCCGTTGCCTCAGATG+AGG + chr3.4:79902857-79902876 MS.gene066826:CDS 60.0%
ACCCCGAACGAAGCCTTTGC+TGG - chr3.4:79902783-79902802 None:intergenic 60.0%
AGGGAGGGAAAGCCATTGGG+AGG + chr3.4:79902707-79902726 MS.gene066826:CDS 60.0%
ATGGCCGGGAAGTTGGAACG+GGG - chr3.4:79902573-79902592 None:intergenic 60.0%
CATGGCCGGGAAGTTGGAAC+GGG - chr3.4:79902574-79902593 None:intergenic 60.0%
CCATGGCCGGGAAGTTGGAA+CGG - chr3.4:79902575-79902594 None:intergenic 60.0%
CGATCCCTTCCACCAATGCC+TGG + chr3.4:79902756-79902775 MS.gene066826:CDS 60.0%
CGCACCAGCTGTGCTTCTCA+GGG - chr3.4:79903365-79903384 None:intergenic 60.0%
CGGCAGGGTTGCTTGAGGAA+TGG + chr3.4:79902658-79902677 MS.gene066826:CDS 60.0%
CGTTCCAACTTCCCGGCCAT+GGG + chr3.4:79902573-79902592 MS.gene066826:CDS 60.0%
GAGCTCCCTGTCAATGGGAC+GGG - chr3.4:79903516-79903535 None:intergenic 60.0%
GCCTTTGCTGGACCAGGCAT+TGG - chr3.4:79902771-79902790 None:intergenic 60.0%
GCTGGAGGCTATGGGGTGAA+TGG + chr3.4:79903162-79903181 MS.gene066826:CDS 60.0%
TAGAGAGCCTTCGTCGCCCA+AGG + chr3.4:79903241-79903260 MS.gene066826:CDS 60.0%
TCGCACCAGCTGTGCTTCTC+AGG - chr3.4:79903366-79903385 None:intergenic 60.0%
TTGCTGACACCACCCATGGC+CGG - chr3.4:79902588-79902607 None:intergenic 60.0%
!! CTGGACCAGGCATTGGTGGA+AGG - chr3.4:79902764-79902783 None:intergenic 60.0%
AACCCCACCTTGGGCGACGA+AGG - chr3.4:79903251-79903270 None:intergenic 65.0%
ACCACCCATGGCCGGGAAGT+TGG - chr3.4:79902580-79902599 None:intergenic 65.0%
AGAGCCTTCGTCGCCCAAGG+TGG + chr3.4:79903244-79903263 MS.gene066826:CDS 65.0%
AGCCTTCGTCGCCCAAGGTG+GGG + chr3.4:79903246-79903265 MS.gene066826:CDS 65.0%
CCGTTCCAACTTCCCGGCCA+TGG + chr3.4:79902572-79902591 MS.gene066826:CDS 65.0%
GAGCCTTCGTCGCCCAAGGT+GGG + chr3.4:79903245-79903264 MS.gene066826:CDS 65.0%
TCCAACTTCCCGGCCATGGG+TGG + chr3.4:79902576-79902595 MS.gene066826:CDS 65.0%
TCTGACCCCGAAGCCGCGTT+TGG - chr3.4:79903630-79903649 None:intergenic 65.0%
TGCTGACACCACCCATGGCC+GGG - chr3.4:79902587-79902606 None:intergenic 65.0%
ACGCGGCTTCGGGGTCAGAG+TGG + chr3.4:79903631-79903650 MS.gene066826:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 79902417 79903713 79902417 ID=MS.gene066826
chr3.4 mRNA 79902417 79903713 79902417 ID=MS.gene066826.t1;Parent=MS.gene066826
chr3.4 exon 79902417 79902907 79902417 ID=MS.gene066826.t1.exon1;Parent=MS.gene066826.t1
chr3.4 CDS 79902417 79902907 79902417 ID=cds.MS.gene066826.t1;Parent=MS.gene066826.t1
chr3.4 exon 79903086 79903713 79903086 ID=MS.gene066826.t1.exon2;Parent=MS.gene066826.t1
chr3.4 CDS 79903086 79903713 79903086 ID=cds.MS.gene066826.t1;Parent=MS.gene066826.t1
Gene Sequence

>MS.gene066826

ATGTGTTTCACCAATTTGGTCAATCAAGATAAATCAACTCATACGCGAAGCAAAAGCAATCATTGTTTAACCGTTGAGGCTCTGGATAAAGATGATAGCCCTATTCTACCTGGGCTTCCTGATGATGTTGCAAAGTATTGCCTTGCACTTGTTCCCCGTTCCAACTTCCCGGCCATGGGTGGTGTCAGCAAGAAATGGAGGTTGTTTATTCGGAGCAAAGAATTTGTGATGGTGCGAAAATCGGCAGGGTTGCTTGAGGAATGGCTCTATTGCTTAACGTTGGACAGTGAAGGGAGGGAAAGCCATTGGGAGGTCATGGATAGTCTTGGTCGCAAATGTCGATCCCTTCCACCAATGCCTGGTCCAGCAAAGGCTTCGTTCGGGGTTGTGGTTCTTAACGGAAAGCTTCTTATCATGGCTGGCTATTCAGCTATTGAAGGAACCGCCGTTGCCTCAGATGAGGTTTACCAATATGATTCTTACCTTAACAGGTATAATTAACTTCTCAAATGTGCATTCTATCTAGAAATCTTTAAGAGTTTATTATGTATATGATAAGTAAGTTCTCCGTCGTAATTTAATTGATGGTAGACCTATCATCCATTTCATAATTTTTGAAACATAATGTTCTAAGTTTTTGACTTGTTCTATTCTCAATTGCAATTGTAGTTGGAGCAGATTATCAAACATGAATGTGGCTCGTTATGACTTCGCTTGTGCCGAAGTGAACGGCTTGGTTTATATTGCTGGAGGCTATGGGGTGAATGGCGACAATCTCTCCAGTGTTGAGATGTACGATCCAGATACTGACAAGTGGACACTGATAGAGAGCCTTCGTCGCCCAAGGTGGGGTTGCTTTGCTTGTGGATTTGAAGATAAGCTCTATGTCATGGGTGGAAGGTCAAGCTTCACTATTGGAAACTCCAAGTTTGTTGATATATATAACCCTGAGAAGCACAGCTGGTGCGAGATTAAGAATGGCTGTGTTATGGTCACAGCTCATGCAGTATTGGAAAAGAAGTTGTTTTGTATTGAGTGGAAAAATCAGCGGAAGCTAGCAATATTCAGTCCTGAAAACAACTCATGGACAATGGTACCCGTCCCATTGACAGGGAGCTCAAGTGTTGGTTTTCGATTCGGGATACTGGACGGAAAGCTGTTGCTATTTCCGGTTGAGAAAGAACCTACTAATCAAACATTGTTATATGATCCAAACGCGGCTTCGGGGTCAGAGTGGCAAACTTCAGACATAAGGCCATCTGGTTTCTGCTTGTGCAGTGTAACAATGAAGGCATGA
Protein sequence

>MS.gene066826.t1

MCFTNLVNQDKSTHTRSKSNHCLTVEALDKDDSPILPGLPDDVAKYCLALVPRSNFPAMGGVSKKWRLFIRSKEFVMVRKSAGLLEEWLYCLTLDSEGRESHWEVMDSLGRKCRSLPPMPGPAKASFGVVVLNGKLLIMAGYSAIEGTAVASDEVYQYDSYLNSWSRLSNMNVARYDFACAEVNGLVYIAGGYGVNGDNLSSVEMYDPDTDKWTLIESLRRPRWGCFACGFEDKLYVMGGRSSFTIGNSKFVDIYNPEKHSWCEIKNGCVMVTAHAVLEKKLFCIEWKNQRKLAIFSPENNSWTMVPVPLTGSSSVGFRFGILDGKLLLFPVEKEPTNQTLLYDPNAASGSEWQTSDIRPSGFCLCSVTMKA