AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050762


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050762.t1 MTR_7g111900 96.835 158 2 2 1 157 1 156 1.56e-106 301
MS.gene050762.t1 MTR_7g103200 85.065 154 21 1 1 154 1 152 4.69e-92 264
MS.gene050762.t1 MTR_1g084200 71.233 146 42 0 1 146 1 146 4.93e-73 216
MS.gene050762.t1 MTR_2g019480 49.254 134 68 0 10 143 14 147 9.26e-49 155
MS.gene050762.t1 MTR_2g046150 47.917 144 74 1 2 145 6 148 5.70e-40 132
MS.gene050762.t1 MTR_4g122930 47.059 153 77 2 1 151 1 151 6.37e-39 129
MS.gene050762.t1 MTR_2g091210 45.528 123 67 0 17 139 12 134 8.12e-34 116
MS.gene050762.t1 MTR_2g091215 53.636 110 51 0 28 137 23 132 1.01e-32 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050762.t1 AT1G56580 66.250 160 48 3 1 154 1 160 1.35e-68 205
MS.gene050762.t1 AT1G09310 65.868 167 43 3 1 154 1 166 3.69e-68 204
MS.gene050762.t1 AT5G46230 47.692 130 68 0 12 141 14 143 3.25e-46 147
MS.gene050762.t1 AT4G24130 45.185 135 74 0 9 143 13 147 4.06e-39 130
MS.gene050762.t1 AT1G30020 43.571 140 77 1 13 150 15 154 8.88e-37 124
MS.gene050762.t1 AT5G49600 46.897 145 67 3 3 138 1 144 4.37e-35 120

Find 0 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACACACAAATTCGAAAAGAT+TGG + 51765:14874-14893 MS.gene050762:CDS 30.0%
CAATTTCAAAAGCAGAAACA+GGG - 51765:15080-15099 MS.gene050762:intergenic 30.0%
CACACAAATTCGAAAAGATT+GGG + 51765:14875-14894 MS.gene050762:CDS 30.0%
TCAATGGTAAAAGACCATTT+GGG - 51765:14786-14805 MS.gene050762:intergenic 30.0%
TCAATTTCAAAAGCAGAAAC+AGG - 51765:15081-15100 MS.gene050762:intergenic 30.0%
TTCAATGGTAAAAGACCATT+TGG - 51765:14787-14806 MS.gene050762:intergenic 30.0%
! AAACTTCTAAAAAGTCCAGA+AGG - 51765:15057-15076 MS.gene050762:intergenic 30.0%
! TTGAATGTGATTTTACCAGT+TGG - 51765:15030-15049 MS.gene050762:intergenic 30.0%
!! AAACCAAAGAGCTTTTGATT+TGG + 51765:14971-14990 MS.gene050762:CDS 30.0%
!!! AACCAAAGAGCTTTTGATTT+GGG + 51765:14972-14991 MS.gene050762:CDS 30.0%
AAAGAAATCTCTCTCCCAAA+TGG + 51765:14769-14788 MS.gene050762:CDS 35.0%
AACCCAAATCAAAAGCTCTT+TGG - 51765:14977-14996 MS.gene050762:intergenic 35.0%
ACTTCAGTGATGTCTTTCAA+TGG - 51765:14802-14821 MS.gene050762:intergenic 35.0%
AGAAATCAAATCCAAATCCG+AGG + 51765:14699-14718 MS.gene050762:CDS 35.0%
ATCACATTCAAAACACCTTC+TGG + 51765:15039-15058 MS.gene050762:CDS 35.0%
GAAGAGAAAGAGAAATCAAG+TGG + 51765:15102-15121 MS.gene050762:CDS 35.0%
GGTAAGATCAAGAAACTGAA+CGG + 51765:14943-14962 MS.gene050762:CDS 35.0%
TTGAAAGACATCACTGAAGT+TGG + 51765:14802-14821 MS.gene050762:CDS 35.0%
! AATGTGATTTTACCAGTTGG+TGG - 51765:15027-15046 MS.gene050762:intergenic 35.0%
! GATTTCTTTGAGAAGCTCTT+TGG - 51765:14758-14777 MS.gene050762:intergenic 35.0%
! TATATTGATGATCCACCAAC+TGG + 51765:15012-15031 MS.gene050762:CDS 35.0%
!!! ACAGAGAAACAGGTTTTGTT+TGG + 51765:14830-14849 MS.gene050762:CDS 35.0%
GTCACTGCTTACGTTGAAAA+TGG + 51765:14922-14941 MS.gene050762:CDS 40.0%
GTTGGTTACAACAGAGAAAC+AGG + 51765:14820-14839 MS.gene050762:CDS 40.0%
TTCAACGTAAGCAGTGACTT+CGG - 51765:14920-14939 MS.gene050762:intergenic 40.0%
!! ATCAAGTGGTGCTACTGTTA+AGG + 51765:15116-15135 MS.gene050762:CDS 40.0%
ACTTCGGTAGCATAGGAAAC+AGG - 51765:14904-14923 MS.gene050762:intergenic 45.0%
TCCAAATCCGAGGTTTACCA+TGG + 51765:14709-14728 MS.gene050762:CDS 45.0%
TCCATGGTAAACCTCGGATT+TGG - 51765:14713-14732 MS.gene050762:intergenic 45.0%
TTCGTCTCCATGGTAAACCT+CGG - 51765:14719-14738 MS.gene050762:intergenic 45.0%
AGCAGTGACTTCGGTAGCAT+AGG - 51765:14911-14930 MS.gene050762:intergenic 50.0%
CCATGGAGACGAAATGTGTC+AGG + 51765:14726-14745 MS.gene050762:CDS 50.0%
CCTGACACATTTCGTCTCCA+TGG - 51765:14729-14748 MS.gene050762:intergenic 50.0%


Chromosome Type Strat End Strand Name
51765 gene 14682 15152 14682 ID=MS.gene050762
51765 mRNA 14682 15152 14682 ID=MS.gene050762.t1;Parent=MS.gene050762
51765 exon 14682 15152 14682 ID=MS.gene050762.t1.exon1;Parent=MS.gene050762.t1
51765 CDS 14682 15152 14682 ID=cds.MS.gene050762.t1;Parent=MS.gene050762.t1
Gene Sequence

>MS.gene050762

ATGTCGTTGGTAACAGAAGAAATCAAATCCAAATCCGAGGTTTACCATGGAGACGAAATGTGTCAGGTGAAATCCAAAGAGCTTCTCAAAGAAATCTCTCTCCCAAATGGTCTTTTACCATTGAAAGACATCACTGAAGTTGGTTACAACAGAGAAACAGGTTTTGTTTGGCTGAAACAGAAGAAAAGCATAACACACAAATTCGAAAAGATTGGGAAACCTGTTTCCTATGCTACCGAAGTCACTGCTTACGTTGAAAATGGTAAGATCAAGAAACTGAACGGTGTTAAAACCAAAGAGCTTTTGATTTGGGTTACTTTGTGTGATATTTATATTGATGATCCACCAACTGGTAAAATCACATTCAAAACACCTTCTGGACTTTTTAGAAGTTTCCCTGTTTCTGCTTTTGAAATTGAAGAAGAGAAAGAGAAATCAAGTGGTGCTACTGTTAAGGAAGCTGCTAACAAG

Protein sequence

>MS.gene050762.t1

MSLVTEEIKSKSEVYHGDEMCQVKSKELLKEISLPNGLLPLKDITEVGYNRETGFVWLKQKKSITHKFEKIGKPVSYATEVTAYVENGKIKKLNGVKTKELLIWVTLCDIYIDDPPTGKITFKTPSGLFRSFPVSAFEIEEEKEKSSGATVKEAANK