AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050763


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050763.t1 MTR_7g111900 96.835 158 2 2 1 157 1 156 1.49e-106 301
MS.gene050763.t1 MTR_7g103200 85.065 154 21 1 1 154 1 152 4.75e-92 264
MS.gene050763.t1 MTR_1g084200 71.233 146 42 0 1 146 1 146 8.47e-73 216
MS.gene050763.t1 MTR_2g019480 49.254 134 68 0 10 143 14 147 1.21e-48 155
MS.gene050763.t1 MTR_2g046150 46.053 152 81 1 2 153 6 156 9.19e-40 132
MS.gene050763.t1 MTR_4g122930 47.059 153 77 2 1 151 1 151 8.99e-39 129
MS.gene050763.t1 MTR_2g091210 45.528 123 67 0 17 139 12 134 7.99e-34 116
MS.gene050763.t1 MTR_2g091215 53.636 110 51 0 28 137 23 132 9.21e-33 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050763.t1 AT1G09310 64.000 175 49 3 1 162 1 174 1.20e-68 206
MS.gene050763.t1 AT1G56580 67.500 160 46 4 1 154 1 160 1.51e-68 206
MS.gene050763.t1 AT5G46230 47.692 130 68 0 12 141 14 143 4.80e-46 147
MS.gene050763.t1 AT4G24130 45.185 135 74 0 9 143 13 147 5.62e-39 130
MS.gene050763.t1 AT1G30020 43.571 140 77 1 13 150 15 154 1.11e-36 124
MS.gene050763.t1 AT5G49600 46.207 145 68 4 3 138 1 144 8.12e-35 120

Find 0 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATTAATAATAAGTTGA+TGG + 51763:8798-8817 MS.gene050763:intron 10.0%
!!! ATTTTCTAGATTATTTATGT+AGG + 51763:8832-8851 MS.gene050763:intron 15.0%
!!! TTTTCTAGATTATTTATGTA+GGG + 51763:8833-8852 MS.gene050763:intron 15.0%
!!! ATCTATATTGATTTATGATG+AGG + 51763:8740-8759 MS.gene050763:intron 20.0%
ACACACAAATTCGAAAAGAT+TGG + 51763:8406-8425 MS.gene050763:CDS 30.0%
CAATTTCAAAAGCAGAAACA+GGG - 51763:8612-8631 None:intergenic 30.0%
CACACAAATTCGAAAAGATT+GGG + 51763:8407-8426 MS.gene050763:CDS 30.0%
TAGATTATTTATGTAGGGCA+GGG + 51763:8838-8857 MS.gene050763:intron 30.0%
TCAATGGTAAAAGACCATTT+GGG - 51763:8318-8337 None:intergenic 30.0%
TCAATTTCAAAAGCAGAAAC+AGG - 51763:8613-8632 None:intergenic 30.0%
TTCAATGGTAAAAGACCATT+TGG - 51763:8319-8338 None:intergenic 30.0%
! AAACTTCTAAAAAGTCCAGA+AGG - 51763:8589-8608 None:intergenic 30.0%
! ATTGATTTATGATGAGGATG+AGG + 51763:8746-8765 MS.gene050763:intron 30.0%
! TTGAATGTGATTTTACCAGT+TGG - 51763:8562-8581 None:intergenic 30.0%
!! AAACCAAAGAGCTTTTGATT+TGG + 51763:8503-8522 MS.gene050763:CDS 30.0%
!!! AACCAAAGAGCTTTTGATTT+GGG + 51763:8504-8523 MS.gene050763:CDS 30.0%
AAAGAAATCTCTCTCCCAAA+TGG + 51763:8301-8320 MS.gene050763:CDS 35.0%
AACCCAAATCAAAAGCTCTT+TGG - 51763:8509-8528 None:intergenic 35.0%
ACTTCAGTGATGTCTTTCAA+TGG - 51763:8334-8353 None:intergenic 35.0%
AGAAATCAAATCCAAATCCG+AGG + 51763:8231-8250 MS.gene050763:CDS 35.0%
ATCACATTCAAAACACCTTC+TGG + 51763:8571-8590 MS.gene050763:CDS 35.0%
CTAGATTATTTATGTAGGGC+AGG + 51763:8837-8856 MS.gene050763:intron 35.0%
GAAGAGAAAGAGAAATCAAG+TGG + 51763:8634-8653 MS.gene050763:CDS 35.0%
GGTAAGATCAAGAAACTGAA+CGG + 51763:8475-8494 MS.gene050763:CDS 35.0%
TTGAAAGACATCACTGAAGT+TGG + 51763:8334-8353 MS.gene050763:CDS 35.0%
! AATGTGATTTTACCAGTTGG+TGG - 51763:8559-8578 None:intergenic 35.0%
! GATTTCTTTGAGAAGCTCTT+TGG - 51763:8290-8309 None:intergenic 35.0%
! TATATTGATGATCCACCAAC+TGG + 51763:8544-8563 MS.gene050763:CDS 35.0%
!!! ACAGAGAAACAGGTTTTGTT+TGG + 51763:8362-8381 MS.gene050763:CDS 35.0%
GTCACTGCTTACGTTGAAAA+TGG + 51763:8454-8473 MS.gene050763:CDS 40.0%
GTTGGTTACAACAGAGAAAC+AGG + 51763:8352-8371 MS.gene050763:CDS 40.0%
TGTTAAGGAAGCTGCTAACA+AGG + 51763:8663-8682 MS.gene050763:CDS 40.0%
TTATGATGAGGATGAGGATG+TGG + 51763:8752-8771 MS.gene050763:intron 40.0%
TTCAACGTAAGCAGTGACTT+CGG - 51763:8452-8471 None:intergenic 40.0%
!! ATCAAGTGGTGCTACTGTTA+AGG + 51763:8648-8667 MS.gene050763:CDS 40.0%
ACTTCGGTAGCATAGGAAAC+AGG - 51763:8436-8455 None:intergenic 45.0%
TCCAAATCCGAGGTTTACCA+TGG + 51763:8241-8260 MS.gene050763:CDS 45.0%
TCCATGGTAAACCTCGGATT+TGG - 51763:8245-8264 None:intergenic 45.0%
TTCGTCTCCATGGTAAACCT+CGG - 51763:8251-8270 None:intergenic 45.0%
! TACTGCTGCTGGTGTTAAAG+AGG + 51763:8705-8724 MS.gene050763:intron 45.0%
AGCAGTGACTTCGGTAGCAT+AGG - 51763:8443-8462 None:intergenic 50.0%
CCATGGAGACGAAATGTGTC+AGG + 51763:8258-8277 MS.gene050763:CDS 50.0%
CCTGACACATTTCGTCTCCA+TGG - 51763:8261-8280 None:intergenic 50.0%
ACTGCTGCTGCTACTGCTGC+TGG + 51763:8694-8713 MS.gene050763:intron 60.0%


Chromosome Type Strat End Strand Name
51763 gene 8214 8889 8214 ID=MS.gene050763
51763 mRNA 8214 8889 8214 ID=MS.gene050763.t1;Parent=MS.gene050763
51763 exon 8214 8684 8214 ID=MS.gene050763.t1.exon1;Parent=MS.gene050763.t1
51763 CDS 8214 8684 8214 ID=cds.MS.gene050763.t1;Parent=MS.gene050763.t1
51763 exon 8854 8889 8854 ID=MS.gene050763.t1.exon2;Parent=MS.gene050763.t1
51763 CDS 8854 8889 8854 ID=cds.MS.gene050763.t1;Parent=MS.gene050763.t1
Gene Sequence

>MS.gene050763

ATGTCGTTGGTAACAGAAGAAATCAAATCCAAATCCGAGGTTTACCATGGAGACGAAATGTGTCAGGTGAAATCCAAAGAGCTTCTCAAAGAAATCTCTCTCCCAAATGGTCTTTTACCATTGAAAGACATCACTGAAGTTGGTTACAACAGAGAAACAGGTTTTGTTTGGCTGAAACAGAAGAAAAGCATAACACACAAATTCGAAAAGATTGGGAAACCTGTTTCCTATGCTACCGAAGTCACTGCTTACGTTGAAAATGGTAAGATCAAGAAACTGAACGGTGTTAAAACCAAAGAGCTTTTGATTTGGGTTACTTTGTGTGATATTTATATTGATGATCCACCAACTGGTAAAATCACATTCAAAACACCTTCTGGACTTTTTAGAAGTTTCCCTGTTTCTGCTTTTGAAATTGAAGAAGAGAAAGAGAAATCAAGTGGTGCTACTGTTAAGGAAGCTGCTAACAAGGTCAATGAAACTGCTGCTGCTACTGCTGCTGGTGTTAAAGAGGTCTAAGTTATAGATCTATATTGATTTATGATGAGGATGAGGATGTGGTTGCTGCTGCTATTAAAATTTCAATTAATTAATAATAAGTTGATGGATTATGTCACTATTTTCTAGATTATTTATGTAGGGCAGGGCAGTTTCAAATCTTAGCTGTGCTATTTGA

Protein sequence

>MS.gene050763.t1

MSLVTEEIKSKSEVYHGDEMCQVKSKELLKEISLPNGLLPLKDITEVGYNRETGFVWLKQKKSITHKFEKIGKPVSYATEVTAYVENGKIKKLNGVKTKELLIWVTLCDIYIDDPPTGKITFKTPSGLFRSFPVSAFEIEEEKEKSSGATVKEAANKGRAVSNLSCAI