Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067187.t1 | XP_013466773.1 | 95.3 | 232 | 11 | 0 | 1 | 232 | 1 | 232 | 2.60E-121 | 444.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067187.t1 | A0A072VHB3 | 95.3 | 232 | 11 | 0 | 1 | 232 | 1 | 232 | 1.9e-121 | 444.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene051997 | MS.gene067187 | PPI |
| MS.gene85263 | MS.gene067187 | PPI |
| MS.gene032192 | MS.gene067187 | PPI |
| MS.gene98743 | MS.gene067187 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067187.t1 | MTR_1g035700 | 95.259 | 232 | 11 | 0 | 1 | 232 | 1 | 232 | 1.78e-168 | 467 |
| MS.gene067187.t1 | MTR_1g035700 | 95.259 | 232 | 11 | 0 | 1 | 232 | 1 | 232 | 1.25e-167 | 467 |
| MS.gene067187.t1 | MTR_1g035700 | 92.469 | 239 | 11 | 1 | 1 | 232 | 1 | 239 | 8.25e-166 | 461 |
| MS.gene067187.t1 | MTR_4g074120 | 60.426 | 235 | 87 | 4 | 1 | 232 | 1 | 232 | 2.93e-92 | 273 |
| MS.gene067187.t1 | MTR_4g074120 | 60.426 | 235 | 87 | 4 | 1 | 232 | 1 | 232 | 2.01e-91 | 272 |
| MS.gene067187.t1 | MTR_2g099155 | 44.000 | 150 | 76 | 4 | 88 | 232 | 38 | 184 | 2.41e-31 | 116 |
| MS.gene067187.t1 | MTR_2g099155 | 44.079 | 152 | 77 | 4 | 86 | 232 | 36 | 184 | 2.49e-31 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067187.t1 | AT1G60670 | 48.039 | 204 | 81 | 8 | 37 | 234 | 1 | 185 | 2.52e-45 | 152 |
| MS.gene067187.t1 | AT1G60670 | 48.039 | 204 | 81 | 8 | 37 | 234 | 1 | 185 | 8.60e-45 | 152 |
| MS.gene067187.t1 | AT1G60670 | 48.039 | 204 | 81 | 8 | 37 | 234 | 1 | 185 | 9.28e-45 | 152 |
| MS.gene067187.t1 | AT1G60670 | 48.020 | 202 | 80 | 8 | 37 | 232 | 1 | 183 | 4.88e-44 | 148 |
| MS.gene067187.t1 | AT1G60670 | 46.269 | 201 | 84 | 6 | 37 | 234 | 1 | 180 | 4.07e-43 | 147 |
| MS.gene067187.t1 | AT1G10820 | 45.918 | 196 | 97 | 5 | 37 | 232 | 1 | 187 | 2.90e-41 | 142 |
| MS.gene067187.t1 | AT1G10820 | 53.103 | 145 | 64 | 3 | 88 | 232 | 21 | 161 | 7.38e-37 | 130 |
| MS.gene067187.t1 | AT3G07565 | 38.710 | 186 | 94 | 3 | 58 | 240 | 41 | 209 | 1.56e-35 | 128 |
| MS.gene067187.t1 | AT3G07565 | 38.503 | 187 | 94 | 4 | 58 | 240 | 41 | 210 | 9.00e-34 | 123 |
| MS.gene067187.t1 | AT3G07565 | 38.547 | 179 | 89 | 4 | 58 | 232 | 41 | 202 | 7.14e-33 | 121 |
| MS.gene067187.t1 | AT3G07565 | 37.838 | 148 | 73 | 2 | 58 | 202 | 41 | 172 | 2.53e-28 | 107 |
| MS.gene067187.t1 | AT1G68160 | 37.339 | 233 | 123 | 6 | 1 | 232 | 3 | 213 | 3.30e-22 | 93.2 |
Find 68 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCCAATGGGAAATTATTT+TGG | 0.109896 | 1.2:+20768844 | MS.gene067187:CDS |
| CACCTTGAAAGTAGATAAAT+TGG | 0.239951 | 1.2:-20778961 | None:intergenic |
| GCATTATACATTATACTTAC+TGG | 0.245013 | 1.2:+20779898 | MS.gene067187:CDS |
| GGGTTGAATCATGATACTTC+TGG | 0.256622 | 1.2:+20768865 | MS.gene067187:CDS |
| CTTCTTGTTGATTCTGTCCC+TGG | 0.270784 | 1.2:+20768973 | MS.gene067187:CDS |
| TGTTGATGAACAGTATAAAT+TGG | 0.341813 | 1.2:+20769032 | MS.gene067187:CDS |
| AGTATCCATATCTATTTCTT+CGG | 0.342122 | 1.2:+20779955 | MS.gene067187:CDS |
| ATATGAATTCATCTTTGAAT+TGG | 0.346328 | 1.2:+20768770 | MS.gene067187:CDS |
| GTATCCATATCTATTTCTTC+GGG | 0.346582 | 1.2:+20779956 | MS.gene067187:CDS |
| ATGATGTATTCGGGGAATTC+AGG | 0.349298 | 1.2:+20768892 | MS.gene067187:CDS |
| TTCCAATTTATCTACTTTCA+AGG | 0.352868 | 1.2:+20778959 | MS.gene067187:CDS |
| TTCTGGGATGATGATGTATT+CGG | 0.353610 | 1.2:+20768882 | MS.gene067187:CDS |
| CACGAATACGTGGCCACACT+TGG | 0.381306 | 1.2:-20773080 | None:intergenic |
| TTCGTCTGTTATAAGTCAAC+CGG | 0.391697 | 1.2:+20768930 | MS.gene067187:CDS |
| CTGTTCGTGATGTTGCTTTG+AGG | 0.405905 | 1.2:+20771902 | MS.gene067187:CDS |
| TTTCCCATTGGAACCATCTC+AGG | 0.412066 | 1.2:-20768835 | None:intergenic |
| TGATCCCGTGAAGCGACTCT+TGG | 0.424126 | 1.2:+20778905 | MS.gene067187:CDS |
| AGTCAACCGGGGATTGCGTC+TGG | 0.427021 | 1.2:+20768943 | MS.gene067187:CDS |
| GGAATTCAGGCGTTGTTAGT+AGG | 0.427819 | 1.2:+20768905 | MS.gene067187:CDS |
| TCTGGGATGATGATGTATTC+GGG | 0.440187 | 1.2:+20768883 | MS.gene067187:CDS |
| GGTTGAATCATGATACTTCT+GGG | 0.449509 | 1.2:+20768866 | MS.gene067187:CDS |
| TAAGCCCGAAGAAATAGATA+TGG | 0.453256 | 1.2:-20779960 | None:intergenic |
| CAACGGATACCATATGATAA+TGG | 0.454642 | 1.2:+20773128 | MS.gene067187:CDS |
| CATGCAATATCATTTGAATC+TGG | 0.456790 | 1.2:+20768793 | MS.gene067187:CDS |
| TGTTAAAGAAGGTCAACAAT+AGG | 0.460190 | 1.2:+20772322 | MS.gene067187:CDS |
| ATTGAAGCATGATGCAGGTT+TGG | 0.461747 | 1.2:+20768996 | MS.gene067187:CDS |
| CCTGCATCATGCTTCAATCC+AGG | 0.467679 | 1.2:-20768991 | None:intergenic |
| GCCACACTTGGGGTTAGAAC+TGG | 0.480931 | 1.2:-20773068 | None:intergenic |
| GTGATGTTGCTTTGAGGTGT+AGG | 0.481867 | 1.2:+20771908 | MS.gene067187:CDS |
| TTCGTGTGTATCACAACCTA+TGG | 0.484014 | 1.2:+20773097 | MS.gene067187:CDS |
| TTATACTGACCATTATCATA+TGG | 0.485193 | 1.2:-20773137 | None:intergenic |
| ATGGCAGCAGAGTCAAATAC+TGG | 0.485703 | 1.2:+20768733 | MS.gene067187:CDS |
| TGCTCCAAGAGTCGCTTCAC+GGG | 0.489073 | 1.2:-20778909 | None:intergenic |
| TGTTAGTAGCTTGCCTGAGA+TGG | 0.491391 | 1.2:+20768822 | MS.gene067187:CDS |
| AACCCAAAATAATTTCCCAT+TGG | 0.495621 | 1.2:-20768847 | None:intergenic |
| ACTGGCTGCATACACTGCTT+TGG | 0.504226 | 1.2:-20773050 | None:intergenic |
| TTGCTCCAAGAGTCGCTTCA+CGG | 0.512660 | 1.2:-20778910 | None:intergenic |
| ACGAATACGTGGCCACACTT+GGG | 0.513683 | 1.2:-20773079 | None:intergenic |
| TGCTTTGGTGATGAAACCAT+TGG | 0.521864 | 1.2:-20773035 | None:intergenic |
| AGAAGAGCACATGTTAAAGA+AGG | 0.529829 | 1.2:+20772311 | MS.gene067187:CDS |
| TTACTGGATGCACTCGCTCA+TGG | 0.531884 | 1.2:+20779914 | MS.gene067187:CDS |
| CCTGGATTGAAGCATGATGC+AGG | 0.532478 | 1.2:+20768991 | MS.gene067187:CDS |
| CTTGCCTGAGATGGTTCCAA+TGG | 0.539348 | 1.2:+20768831 | MS.gene067187:CDS |
| GAACAGCCAGACGCAATCCC+CGG | 0.541451 | 1.2:-20768949 | None:intergenic |
| TTCACGTACCTCATGATACT+CGG | 0.542623 | 1.2:-20771853 | None:intergenic |
| ATCCGTTGATTTCTGTCCAT+AGG | 0.552463 | 1.2:-20773113 | None:intergenic |
| AACAGTATAAATTGGAGGAG+GGG | 0.559930 | 1.2:+20769040 | MS.gene067187:CDS |
| TCTTCTTCAGGATAAGCCAA+TGG | 0.562507 | 1.2:+20773019 | MS.gene067187:intron |
| TCATGGCAACTTGTCAAGTG+TGG | 0.564987 | 1.2:+20779931 | MS.gene067187:CDS |
| CGAATACGTGGCCACACTTG+GGG | 0.565432 | 1.2:-20773078 | None:intergenic |
| TGAACAGTATAAATTGGAGG+AGG | 0.569871 | 1.2:+20769038 | MS.gene067187:CDS |
| TTGCCTGAGATGGTTCCAAT+GGG | 0.573001 | 1.2:+20768832 | MS.gene067187:CDS |
| CTGCATCATGCTTCAATCCA+GGG | 0.583717 | 1.2:-20768990 | None:intergenic |
| CATGGCAACTTGTCAAGTGT+GGG | 0.584793 | 1.2:+20779932 | MS.gene067187:CDS |
| TGATGAACAGTATAAATTGG+AGG | 0.605084 | 1.2:+20769035 | MS.gene067187:CDS |
| GAACAGTATAAATTGGAGGA+GGG | 0.605834 | 1.2:+20769039 | MS.gene067187:CDS |
| TCGTCTGTTATAAGTCAACC+GGG | 0.612438 | 1.2:+20768931 | MS.gene067187:CDS |
| ATGCAGGTTTGGCTGCTGAG+TGG | 0.614045 | 1.2:+20769007 | MS.gene067187:CDS |
| ATGTTGCTTTGAGGTGTAGG+TGG | 0.619616 | 1.2:+20771911 | MS.gene067187:CDS |
| AGTATAATGTATAATGCATG+AGG | 0.627142 | 1.2:-20779892 | None:intergenic |
| AAAGAAGGTCAACAATAGGA+AGG | 0.632079 | 1.2:+20772326 | MS.gene067187:CDS |
| GCCAGTTCTAACCCCAAGTG+TGG | 0.637597 | 1.2:+20773067 | MS.gene067187:CDS |
| GCAATATCATTTGAATCTGG+TGG | 0.655444 | 1.2:+20768796 | MS.gene067187:CDS |
| TTGTGATACACACGAATACG+TGG | 0.661179 | 1.2:-20773090 | None:intergenic |
| CTGGGATGATGATGTATTCG+GGG | 0.667463 | 1.2:+20768884 | MS.gene067187:CDS |
| AACCTATGGACAGAAATCAA+CGG | 0.673847 | 1.2:+20773111 | MS.gene067187:CDS |
| CTGATGAGCCGAGTATCATG+AGG | 0.688164 | 1.2:+20771845 | MS.gene067187:CDS |
| CGTCTGTTATAAGTCAACCG+GGG | 0.721896 | 1.2:+20768932 | MS.gene067187:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 20768733 | 20779996 | 20768733 | ID=MS.gene067187 |
| chr1.2 | mRNA | 20768733 | 20779996 | 20768733 | ID=MS.gene067187.t1;Parent=MS.gene067187 |
| chr1.2 | exon | 20768733 | 20769070 | 20768733 | ID=MS.gene067187.t1.exon1;Parent=MS.gene067187.t1 |
| chr1.2 | CDS | 20768733 | 20769070 | 20768733 | ID=cds.MS.gene067187.t1;Parent=MS.gene067187.t1 |
| chr1.2 | exon | 20771840 | 20771939 | 20771840 | ID=MS.gene067187.t1.exon2;Parent=MS.gene067187.t1 |
| chr1.2 | CDS | 20771840 | 20771939 | 20771840 | ID=cds.MS.gene067187.t1;Parent=MS.gene067187.t1 |
| chr1.2 | exon | 20772294 | 20772347 | 20772294 | ID=MS.gene067187.t1.exon3;Parent=MS.gene067187.t1 |
| chr1.2 | CDS | 20772294 | 20772347 | 20772294 | ID=cds.MS.gene067187.t1;Parent=MS.gene067187.t1 |
| chr1.2 | exon | 20773029 | 20773149 | 20773029 | ID=MS.gene067187.t1.exon4;Parent=MS.gene067187.t1 |
| chr1.2 | CDS | 20773029 | 20773149 | 20773029 | ID=cds.MS.gene067187.t1;Parent=MS.gene067187.t1 |
| chr1.2 | exon | 20778898 | 20778980 | 20778898 | ID=MS.gene067187.t1.exon5;Parent=MS.gene067187.t1 |
| chr1.2 | CDS | 20778898 | 20778980 | 20778898 | ID=cds.MS.gene067187.t1;Parent=MS.gene067187.t1 |
| chr1.2 | exon | 20779892 | 20779996 | 20779892 | ID=MS.gene067187.t1.exon6;Parent=MS.gene067187.t1 |
| chr1.2 | CDS | 20779892 | 20779996 | 20779892 | ID=cds.MS.gene067187.t1;Parent=MS.gene067187.t1 |
| Gene Sequence |
| Protein sequence |
