Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067527.t1 | XP_013459273.1 | 91.6 | 83 | 7 | 0 | 69 | 151 | 115 | 197 | 5.80E-33 | 150.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067527.t1 | A0A072UTZ0 | 91.6 | 83 | 7 | 0 | 69 | 151 | 115 | 197 | 4.2e-33 | 150.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054236 | MS.gene067527 | 0.801565 | 8.48E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene067527 | 0.810654 | 1.04E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene067527 | 0.800917 | 1.15E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067527.t1 | MTR_3g435250 | 91.566 | 83 | 7 | 0 | 69 | 151 | 115 | 197 | 2.31e-49 | 157 |
| MS.gene067527.t1 | MTR_3g435230 | 79.518 | 83 | 17 | 0 | 69 | 151 | 115 | 197 | 2.01e-41 | 137 |
| MS.gene067527.t1 | MTR_3g435200 | 68.675 | 83 | 26 | 0 | 69 | 151 | 113 | 195 | 6.43e-36 | 123 |
| MS.gene067527.t1 | MTR_3g435170 | 77.465 | 71 | 16 | 0 | 69 | 139 | 115 | 185 | 4.25e-34 | 118 |
| MS.gene067527.t1 | MTR_5g041930 | 55.422 | 83 | 37 | 0 | 69 | 151 | 120 | 202 | 2.21e-29 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067527.t1 | AT1G18720 | 60.000 | 90 | 36 | 0 | 60 | 149 | 113 | 202 | 8.58e-33 | 115 |
| MS.gene067527.t1 | AT1G18720 | 60.000 | 90 | 36 | 0 | 60 | 149 | 113 | 202 | 8.58e-33 | 115 |
| MS.gene067527.t1 | AT1G74440 | 58.974 | 78 | 32 | 0 | 67 | 144 | 120 | 197 | 8.83e-28 | 102 |
| MS.gene067527.t1 | AT1G74440 | 58.974 | 78 | 32 | 0 | 67 | 144 | 120 | 197 | 8.83e-28 | 102 |
Find 44 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCCTTCAATTCTTTGATAT+TGG | 0.256590 | 3.2:+26519625 | None:intergenic |
| TTTGTCTGAAATCCAGGATA+TGG | 0.368016 | 3.2:+26519668 | None:intergenic |
| TAGCTAGACTCTGCTCCTTT+AGG | 0.375909 | 3.2:-26520398 | MS.gene067527:CDS |
| GTGTTGGCTGCTCAGTTGAT+TGG | 0.382102 | 3.2:-26519982 | MS.gene067527:CDS |
| CTCAGACTCCATCTCGAATA+CGG | 0.422561 | 3.2:-26520422 | MS.gene067527:CDS |
| AGCCAATATCAAAGAATTGA+AGG | 0.427037 | 3.2:-26519627 | MS.gene067527:CDS |
| TTCACCTTTGTCTGAAATCC+AGG | 0.431535 | 3.2:+26519662 | None:intergenic |
| TATAATCTTTCAGGTTGTGT+TGG | 0.431542 | 3.2:-26519998 | MS.gene067527:intron |
| GGATGGACTGGAACATCTAT+TGG | 0.439686 | 3.2:-26519961 | MS.gene067527:CDS |
| ACACGCAACAATCAAGATAA+TGG | 0.443180 | 3.2:+26520530 | None:intergenic |
| GTTCATATCCAATGGATGAT+TGG | 0.446343 | 3.2:+26519690 | None:intergenic |
| ATTGGATATGAACCATATCC+TGG | 0.460320 | 3.2:-26519680 | MS.gene067527:CDS |
| TATTGGTCATGGCGTGTTTG+AGG | 0.463494 | 3.2:-26519944 | MS.gene067527:intron |
| TGGCTGCTCAGTTGATTGGA+TGG | 0.484630 | 3.2:-26519978 | MS.gene067527:CDS |
| CAAAAGCGCCACGGCTAATG+AGG | 0.486119 | 3.2:-26520470 | MS.gene067527:CDS |
| ACCTCAAGAATCACGAAGAA+AGG | 0.495722 | 3.2:+26519792 | None:intergenic |
| GCTCAGTTGATTGGATGGAC+TGG | 0.499989 | 3.2:-26519973 | MS.gene067527:CDS |
| CGATTGGATGACCTTGCTCA+AGG | 0.509060 | 3.2:-26519828 | MS.gene067527:CDS |
| GAAACGTGTGTCAACTCGAT+TGG | 0.511678 | 3.2:-26519844 | MS.gene067527:CDS |
| GCCTTTCTTCGTGATTCTTG+AGG | 0.516103 | 3.2:-26519793 | MS.gene067527:intron |
| TAGGTCCTCCAATCATCCAT+TGG | 0.519762 | 3.2:-26519698 | MS.gene067527:CDS |
| ATACACCCACACATTGCATA+AGG | 0.531468 | 3.2:+26520503 | None:intergenic |
| CCTCCTGTCATGACTCCTAA+AGG | 0.532466 | 3.2:+26520383 | None:intergenic |
| TCAGACTCCATCTCGAATAC+GGG | 0.539823 | 3.2:-26520421 | MS.gene067527:CDS |
| CTCTTCTAGAGATTTACTCT+CGG | 0.555374 | 3.2:-26520358 | MS.gene067527:intron |
| AGGATATGGTTCATATCCAA+TGG | 0.559473 | 3.2:+26519682 | None:intergenic |
| CTAGCTACCCGTATTCGAGA+TGG | 0.580045 | 3.2:+26520414 | None:intergenic |
| CATATCCAATGGATGATTGG+AGG | 0.588211 | 3.2:+26519693 | None:intergenic |
| GTTCATCCTTATGCAATGTG+TGG | 0.592080 | 3.2:-26520509 | MS.gene067527:CDS |
| TTCAGACAAAGGTGAAGGCA+AGG | 0.592728 | 3.2:-26519655 | MS.gene067527:CDS |
| GAGAGTAAATCTCTAGAAGA+GGG | 0.596002 | 3.2:+26520360 | None:intergenic |
| GCTCCTTTAGGAGTCATGAC+AGG | 0.609411 | 3.2:-26520386 | MS.gene067527:CDS |
| CGCAACAATCAAGATAATGG+TGG | 0.613144 | 3.2:+26520533 | None:intergenic |
| TTCATCCTTATGCAATGTGT+GGG | 0.617017 | 3.2:-26520508 | MS.gene067527:CDS |
| CGAGAGTAAATCTCTAGAAG+AGG | 0.617070 | 3.2:+26520359 | None:intergenic |
| ACTGGAACATCTATTGGTCA+TGG | 0.617255 | 3.2:-26519955 | MS.gene067527:CDS |
| TGGATTTCAGACAAAGGTGA+AGG | 0.621966 | 3.2:-26519660 | MS.gene067527:CDS |
| CGTATTCGAGATGGAGTCTG+AGG | 0.625280 | 3.2:+26520423 | None:intergenic |
| AGGTCTTGCCTCATTAGCCG+TGG | 0.638460 | 3.2:+26520462 | None:intergenic |
| AAGACCTAGTGAAAATGCAA+TGG | 0.638726 | 3.2:-26520446 | MS.gene067527:CDS |
| GCAACAATCAAGATAATGGT+GGG | 0.643515 | 3.2:+26520534 | None:intergenic |
| CCTTTAGGAGTCATGACAGG+AGG | 0.670470 | 3.2:-26520383 | MS.gene067527:CDS |
| TGAGCGAGTCAAAAGCGCCA+CGG | 0.688410 | 3.2:-26520479 | MS.gene067527:CDS |
| ATATCCTGGATTTCAGACAA+AGG | 0.705635 | 3.2:-26519666 | MS.gene067527:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GACTATTTTAATTTGTTTGT+AGG | - | chr3.2:26520424-26520443 | MS.gene067527:CDS | 20.0% |
| ! | CAATTGTTGTGCTTTAATTA+TGG | - | chr3.2:26520118-26520137 | MS.gene067527:intron | 25.0% |
| ! | TTGCTTTCAAAATGAAAACA+AGG | - | chr3.2:26520399-26520418 | MS.gene067527:CDS | 25.0% |
| !! | ATTATGGACTATAATCTTTC+AGG | - | chr3.2:26520134-26520153 | MS.gene067527:intron | 25.0% |
| !! | TCGTTTTACTCACTTATATA+TGG | + | chr3.2:26519958-26519977 | None:intergenic | 25.0% |
| ACAAAAGATGTTTATGACGA+AGG | - | chr3.2:26520064-26520083 | MS.gene067527:intron | 30.0% | |
| AGCCAATATCAAAGAATTGA+AGG | - | chr3.2:26520514-26520533 | MS.gene067527:CDS | 30.0% | |
| ATGTTAGGCTACATTCATTA+TGG | - | chr3.2:26519892-26519911 | MS.gene067527:intron | 30.0% | |
| GTCCTTCAATTCTTTGATAT+TGG | + | chr3.2:26520519-26520538 | None:intergenic | 30.0% | |
| TACTCACTTATATATGGATG+TGG | + | chr3.2:26519952-26519971 | None:intergenic | 30.0% | |
| TGTAACAGCTTAAATTGAGA+CGG | + | chr3.2:26519851-26519870 | None:intergenic | 30.0% | |
| TGTTAGGCTACATTCATTAT+GGG | - | chr3.2:26519893-26519912 | MS.gene067527:intron | 30.0% | |
| TTAAATTGAGACGGACAAAT+TGG | + | chr3.2:26519842-26519861 | None:intergenic | 30.0% | |
| !! | TATAATCTTTCAGGTTGTGT+TGG | - | chr3.2:26520143-26520162 | MS.gene067527:intron | 30.0% |
| AAGACCTAGTGAAAATGCAA+TGG | - | chr3.2:26519695-26519714 | MS.gene067527:CDS | 35.0% | |
| ACACGCAACAATCAAGATAA+TGG | + | chr3.2:26519614-26519633 | None:intergenic | 35.0% | |
| AGGATATGGTTCATATCCAA+TGG | + | chr3.2:26520462-26520481 | None:intergenic | 35.0% | |
| ATATCCTGGATTTCAGACAA+AGG | - | chr3.2:26520475-26520494 | MS.gene067527:CDS | 35.0% | |
| ATTGGATATGAACCATATCC+TGG | - | chr3.2:26520461-26520480 | MS.gene067527:CDS | 35.0% | |
| CCATTAAAAAACCTTGAGCA+AGG | + | chr3.2:26520327-26520346 | None:intergenic | 35.0% | |
| GTTCATATCCAATGGATGAT+TGG | + | chr3.2:26520454-26520473 | None:intergenic | 35.0% | |
| TTCATCCTTATGCAATGTGT+GGG | - | chr3.2:26519633-26519652 | MS.gene067527:CDS | 35.0% | |
| ! | CTCTTCTAGAGATTTACTCT+CGG | - | chr3.2:26519783-26519802 | MS.gene067527:intron | 35.0% |
| ! | GAGAGTAAATCTCTAGAAGA+GGG | + | chr3.2:26519784-26519803 | None:intergenic | 35.0% |
| ! | GCAACAATCAAGATAATGGT+GGG | + | chr3.2:26519610-26519629 | None:intergenic | 35.0% |
| ! | TTTGTCTGAAATCCAGGATA+TGG | + | chr3.2:26520476-26520495 | None:intergenic | 35.0% |
| !!! | CCTTGCTCAAGGTTTTTTAA+TGG | - | chr3.2:26520324-26520343 | MS.gene067527:intron | 35.0% |
| AAATTGGCAACAAGCTTGAC+AGG | + | chr3.2:26519826-26519845 | None:intergenic | 40.0% | |
| ACCTCAAGAATCACGAAGAA+AGG | + | chr3.2:26520352-26520371 | None:intergenic | 40.0% | |
| ACTGGAACATCTATTGGTCA+TGG | - | chr3.2:26520186-26520205 | MS.gene067527:intron | 40.0% | |
| ATACACCCACACATTGCATA+AGG | + | chr3.2:26519641-26519660 | None:intergenic | 40.0% | |
| CATATCCAATGGATGATTGG+AGG | + | chr3.2:26520451-26520470 | None:intergenic | 40.0% | |
| CTACATTCATTATGGGAGAG+GGG | - | chr3.2:26519900-26519919 | MS.gene067527:intron | 40.0% | |
| GCTACATTCATTATGGGAGA+GGG | - | chr3.2:26519899-26519918 | MS.gene067527:intron | 40.0% | |
| GTTCATCCTTATGCAATGTG+TGG | - | chr3.2:26519632-26519651 | MS.gene067527:CDS | 40.0% | |
| GTTTGTCATAGTGCTTGAAC+TGG | - | chr3.2:26520376-26520395 | MS.gene067527:CDS | 40.0% | |
| TCATGTGACGATGTGATGTT+AGG | - | chr3.2:26519877-26519896 | MS.gene067527:intron | 40.0% | |
| TGGATTTCAGACAAAGGTGA+AGG | - | chr3.2:26520481-26520500 | MS.gene067527:CDS | 40.0% | |
| ! | CGAGAGTAAATCTCTAGAAG+AGG | + | chr3.2:26519785-26519804 | None:intergenic | 40.0% |
| ! | CGCAACAATCAAGATAATGG+TGG | + | chr3.2:26519611-26519630 | None:intergenic | 40.0% |
| ! | TTCACCTTTGTCTGAAATCC+AGG | + | chr3.2:26520482-26520501 | None:intergenic | 40.0% |
| !! | TGCTTTTCAGTGATGGAACA+AGG | + | chr3.2:26520228-26520247 | None:intergenic | 40.0% |
| CTCAGACTCCATCTCGAATA+CGG | - | chr3.2:26519719-26519738 | MS.gene067527:intron | 45.0% | |
| GAAACGTGTGTCAACTCGAT+TGG | - | chr3.2:26520297-26520316 | MS.gene067527:intron | 45.0% | |
| GCCTTTCTTCGTGATTCTTG+AGG | - | chr3.2:26520348-26520367 | MS.gene067527:intron | 45.0% | |
| GGCTACATTCATTATGGGAG+AGG | - | chr3.2:26519898-26519917 | MS.gene067527:intron | 45.0% | |
| TAGCTAGACTCTGCTCCTTT+AGG | - | chr3.2:26519743-26519762 | MS.gene067527:intron | 45.0% | |
| TAGGTCCTCCAATCATCCAT+TGG | - | chr3.2:26520443-26520462 | MS.gene067527:CDS | 45.0% | |
| TATTGGTCATGGCGTGTTTG+AGG | - | chr3.2:26520197-26520216 | MS.gene067527:intron | 45.0% | |
| TCAGACTCCATCTCGAATAC+GGG | - | chr3.2:26519720-26519739 | MS.gene067527:intron | 45.0% | |
| TTCAGACAAAGGTGAAGGCA+AGG | - | chr3.2:26520486-26520505 | MS.gene067527:CDS | 45.0% | |
| ! | GAGGCCATTGCATTTTCACT+AGG | + | chr3.2:26519702-26519721 | None:intergenic | 45.0% |
| ! | TCCATCACTGAAAAGCACGA+CGG | - | chr3.2:26520231-26520250 | MS.gene067527:intron | 45.0% |
| !! | GGATGGACTGGAACATCTAT+TGG | - | chr3.2:26520180-26520199 | MS.gene067527:intron | 45.0% |
| CCTCCTGTCATGACTCCTAA+AGG | + | chr3.2:26519761-26519780 | None:intergenic | 50.0% | |
| CGATTGGATGACCTTGCTCA+AGG | - | chr3.2:26520313-26520332 | MS.gene067527:intron | 50.0% | |
| CGTATTCGAGATGGAGTCTG+AGG | + | chr3.2:26519721-26519740 | None:intergenic | 50.0% | |
| CTAGCTACCCGTATTCGAGA+TGG | + | chr3.2:26519730-26519749 | None:intergenic | 50.0% | |
| GCAACAAGCTTGACAGGTGA+GGG | + | chr3.2:26519820-26519839 | None:intergenic | 50.0% | |
| GCTCAGTTGATTGGATGGAC+TGG | - | chr3.2:26520168-26520187 | MS.gene067527:intron | 50.0% | |
| TGGCTGCTCAGTTGATTGGA+TGG | - | chr3.2:26520163-26520182 | MS.gene067527:intron | 50.0% | |
| ! | CCTTTAGGAGTCATGACAGG+AGG | - | chr3.2:26519758-26519777 | MS.gene067527:intron | 50.0% |
| ! | GCTCCTTTAGGAGTCATGAC+AGG | - | chr3.2:26519755-26519774 | MS.gene067527:intron | 50.0% |
| ! | TCATTATGGGAGAGGGGTAC+TGG | - | chr3.2:26519906-26519925 | MS.gene067527:intron | 50.0% |
| ! | TCCGTCGTGCTTTTCAGTGA+TGG | + | chr3.2:26520235-26520254 | None:intergenic | 50.0% |
| !! | CATTATGGGAGAGGGGTACT+GGG | - | chr3.2:26519907-26519926 | MS.gene067527:intron | 50.0% |
| !! | GTGTTGGCTGCTCAGTTGAT+TGG | - | chr3.2:26520159-26520178 | MS.gene067527:intron | 50.0% |
| AGGTCTTGCCTCATTAGCCG+TGG | + | chr3.2:26519682-26519701 | None:intergenic | 55.0% | |
| GGCAACAAGCTTGACAGGTG+AGG | + | chr3.2:26519821-26519840 | None:intergenic | 55.0% | |
| !! | CAAAAGCGCCACGGCTAATG+AGG | - | chr3.2:26519671-26519690 | MS.gene067527:CDS | 55.0% |
| !! | TGAGCGAGTCAAAAGCGCCA+CGG | - | chr3.2:26519662-26519681 | MS.gene067527:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 26519601 | 26520562 | 26519601 | ID=MS.gene067527 |
| chr3.2 | mRNA | 26519601 | 26520562 | 26519601 | ID=MS.gene067527.t1;Parent=MS.gene067527 |
| chr3.2 | exon | 26520359 | 26520562 | 26520359 | ID=MS.gene067527.t1.exon1;Parent=MS.gene067527.t1 |
| chr3.2 | CDS | 26520359 | 26520562 | 26520359 | ID=cds.MS.gene067527.t1;Parent=MS.gene067527.t1 |
| chr3.2 | exon | 26519945 | 26520007 | 26519945 | ID=MS.gene067527.t1.exon2;Parent=MS.gene067527.t1 |
| chr3.2 | CDS | 26519945 | 26520007 | 26519945 | ID=cds.MS.gene067527.t1;Parent=MS.gene067527.t1 |
| chr3.2 | exon | 26519794 | 26519865 | 26519794 | ID=MS.gene067527.t1.exon3;Parent=MS.gene067527.t1 |
| chr3.2 | CDS | 26519794 | 26519865 | 26519794 | ID=cds.MS.gene067527.t1;Parent=MS.gene067527.t1 |
| chr3.2 | exon | 26519601 | 26519717 | 26519601 | ID=MS.gene067527.t1.exon4;Parent=MS.gene067527.t1 |
| chr3.2 | CDS | 26519601 | 26519717 | 26519601 | ID=cds.MS.gene067527.t1;Parent=MS.gene067527.t1 |
| Gene Sequence |
| Protein sequence |
