Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067914.t1 | XP_024627753.1 | 66.4 | 128 | 12 | 1 | 1 | 97 | 1 | 128 | 5.90E-34 | 153.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067914.t1 | B7FH71 | 63.6 | 129 | 15 | 2 | 1 | 97 | 1 | 129 | 8.2e-30 | 139.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050363 | MS.gene067914 | 0.821766 | 3.39E-53 | -1.69E-46 |
| MS.gene051946 | MS.gene067914 | 0.807623 | 4.62E-50 | -1.69E-46 |
| MS.gene053549 | MS.gene067914 | 0.816785 | 4.62E-52 | -1.69E-46 |
| MS.gene055401 | MS.gene067914 | 0.800423 | 1.45E-48 | -1.69E-46 |
| MS.gene057014 | MS.gene067914 | 0.804601 | 2.00E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCCACCTGGGCCTGATGC+TGG | 0.216285 | 8.3:+33825375 | MS.gene067914:CDS |
| GGGGAAGATGTGTTTGGGCT+TGG | 0.239614 | 8.3:+33825420 | MS.gene067914:CDS |
| GGGAAGATGTGTTTGGGCTT+GGG | 0.240843 | 8.3:+33825421 | MS.gene067914:CDS |
| GTTTGTGATATTAGGTCTTT+TGG | 0.255388 | 8.3:-33825583 | None:intergenic |
| TGCTCTAGTCCGGGCCCACC+TGG | 0.276732 | 8.3:+33825363 | MS.gene067914:CDS |
| ATCAAATTGTTTGTGATATT+AGG | 0.276758 | 8.3:-33825591 | None:intergenic |
| GCTGGTGGGGAAGATGTGTT+TGG | 0.322107 | 8.3:+33825414 | MS.gene067914:CDS |
| AGCTCCTGGCTGCTCTAGTC+CGG | 0.331077 | 8.3:+33825353 | MS.gene067914:CDS |
| TGCTGGAATCTTTGTGTCCA+TGG | 0.342466 | 8.3:-33825274 | None:intergenic |
| GATGTGTTTGGGCTTGGGCC+TGG | 0.365493 | 8.3:+33825426 | MS.gene067914:CDS |
| CCTTCAACTTCTTCTTCTCC+AGG | 0.374999 | 8.3:-33825444 | None:intergenic |
| GCCCACCTGGGCCTGATGCT+GGG | 0.385695 | 8.3:+33825376 | MS.gene067914:CDS |
| GCAGAGGGTGACTCAGCTCC+TGG | 0.403105 | 8.3:+33825339 | MS.gene067914:CDS |
| CTATTAGAGCCTGGTGCTGG+TGG | 0.429246 | 8.3:+33825399 | MS.gene067914:CDS |
| GAAGTTGAAGGAGATATTGC+AGG | 0.434197 | 8.3:+33825456 | MS.gene067914:CDS |
| CTGGTGGGGAAGATGTGTTT+GGG | 0.437212 | 8.3:+33825415 | MS.gene067914:CDS |
| TGAAGGAGATATTGCAGGAG+AGG | 0.441071 | 8.3:+33825461 | MS.gene067914:CDS |
| GATATTGCAGGAGAGGTTGT+AGG | 0.444704 | 8.3:+33825468 | MS.gene067914:CDS |
| TATTAGAGCCTGGTGCTGGT+GGG | 0.447878 | 8.3:+33825400 | MS.gene067914:CDS |
| GGCTCTAATAGCCCAGCATC+AGG | 0.456286 | 8.3:-33825387 | None:intergenic |
| GCATCAGGCCCAGGTGGGCC+CGG | 0.462450 | 8.3:-33825372 | None:intergenic |
| GCTCCTGGCTGCTCTAGTCC+GGG | 0.489743 | 8.3:+33825354 | MS.gene067914:CDS |
| GGGCTATTAGAGCCTGGTGC+TGG | 0.490149 | 8.3:+33825396 | MS.gene067914:CDS |
| ATTAGAGCCTGGTGCTGGTG+GGG | 0.496717 | 8.3:+33825401 | MS.gene067914:CDS |
| GCTCTAGTCCGGGCCCACCT+GGG | 0.506944 | 8.3:+33825364 | MS.gene067914:CDS |
| GATGCTGGGCTATTAGAGCC+TGG | 0.508970 | 8.3:+33825390 | MS.gene067914:CDS |
| CCTGGAGAAGAAGAAGTTGA+AGG | 0.541963 | 8.3:+33825444 | MS.gene067914:CDS |
| AATAGCCCAGCATCAGGCCC+AGG | 0.552354 | 8.3:-33825381 | None:intergenic |
| AGCCCAGCATCAGGCCCAGG+TGG | 0.566917 | 8.3:-33825378 | None:intergenic |
| GGGCCCGGACTAGAGCAGCC+AGG | 0.581693 | 8.3:-33825357 | None:intergenic |
| AGTAACAGCAACAAAAGTGT+TGG | 0.609923 | 8.3:+33825302 | MS.gene067914:CDS |
| ACATCTTCCCCACCAGCACC+AGG | 0.610370 | 8.3:-33825408 | None:intergenic |
| GTGATAGAGAATGCAGCAGA+GGG | 0.615284 | 8.3:+33825324 | MS.gene067914:CDS |
| GCCCAGCATCAGGCCCAGGT+GGG | 0.625332 | 8.3:-33825377 | None:intergenic |
| GGTGATAGAGAATGCAGCAG+AGG | 0.711837 | 8.3:+33825323 | MS.gene067914:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAATTGTTTGTGATATT+AGG | - | chr8.3:33825594-33825613 | None:intergenic | 20.0% |
| !! | TCAAAAAACATATACAACAT+GGG | - | chr8.3:33825627-33825646 | None:intergenic | 20.0% |
| ! | CTCAAAAAACATATACAACA+TGG | - | chr8.3:33825628-33825647 | None:intergenic | 25.0% |
| !!! | CATGTTGTATATGTTTTTTG+AGG | + | chr8.3:33825626-33825645 | MS.gene067914:CDS | 25.0% |
| ! | ATTTGGTGATAATGATTGTG+TGG | + | chr8.3:33825524-33825543 | MS.gene067914:intron | 30.0% |
| ! | GTTTGTGATATTAGGTCTTT+TGG | - | chr8.3:33825586-33825605 | None:intergenic | 30.0% |
| ! | TTTGGTGATAATGATTGTGT+GGG | + | chr8.3:33825525-33825544 | MS.gene067914:intron | 30.0% |
| ! | AGTAACAGCAACAAAAGTGT+TGG | + | chr8.3:33825302-33825321 | MS.gene067914:CDS | 35.0% |
| GAAGTTGAAGGAGATATTGC+AGG | + | chr8.3:33825456-33825475 | MS.gene067914:CDS | 40.0% | |
| GATAATGATTGTGTGGGTGA+TGG | + | chr8.3:33825531-33825550 | MS.gene067914:intron | 40.0% | |
| ! | AGAGGTTGTAGGTGATTTTG+TGG | + | chr8.3:33825479-33825498 | MS.gene067914:intron | 40.0% |
| ! | GTTGTAGGTGATTTTGTGGA+AGG | + | chr8.3:33825483-33825502 | MS.gene067914:intron | 40.0% |
| CCTGGAGAAGAAGAAGTTGA+AGG | + | chr8.3:33825444-33825463 | MS.gene067914:CDS | 45.0% | |
| CCTTCAACTTCTTCTTCTCC+AGG | - | chr8.3:33825447-33825466 | None:intergenic | 45.0% | |
| GATATTGCAGGAGAGGTTGT+AGG | + | chr8.3:33825468-33825487 | MS.gene067914:CDS | 45.0% | |
| GTGATAGAGAATGCAGCAGA+GGG | + | chr8.3:33825324-33825343 | MS.gene067914:CDS | 45.0% | |
| TGAAGGAGATATTGCAGGAG+AGG | + | chr8.3:33825461-33825480 | MS.gene067914:CDS | 45.0% | |
| ! | GGTGATTTTGTGGAAGGTGA+TGG | + | chr8.3:33825489-33825508 | MS.gene067914:intron | 45.0% |
| !! | GTTGCTGTTACTGCTTTTGC+TGG | - | chr8.3:33825294-33825313 | None:intergenic | 45.0% |
| CTGGTGGGGAAGATGTGTTT+GGG | + | chr8.3:33825415-33825434 | MS.gene067914:CDS | 50.0% | |
| GGGAAGATGTGTTTGGGCTT+GGG | + | chr8.3:33825421-33825440 | MS.gene067914:CDS | 50.0% | |
| GGTGATAGAGAATGCAGCAG+AGG | + | chr8.3:33825323-33825342 | MS.gene067914:CDS | 50.0% | |
| ! | GATGGAGCTGGTGCTGAATT+TGG | + | chr8.3:33825507-33825526 | MS.gene067914:intron | 50.0% |
| !! | TATTAGAGCCTGGTGCTGGT+GGG | + | chr8.3:33825400-33825419 | MS.gene067914:CDS | 50.0% |
| !! | TTTGTGGAAGGTGATGGAGC+TGG | + | chr8.3:33825495-33825514 | MS.gene067914:intron | 50.0% |
| GATGCTGGGCTATTAGAGCC+TGG | + | chr8.3:33825390-33825409 | MS.gene067914:CDS | 55.0% | |
| GCTGGTGGGGAAGATGTGTT+TGG | + | chr8.3:33825414-33825433 | MS.gene067914:CDS | 55.0% | |
| GGGGAAGATGTGTTTGGGCT+TGG | + | chr8.3:33825420-33825439 | MS.gene067914:CDS | 55.0% | |
| ! | CTATTAGAGCCTGGTGCTGG+TGG | + | chr8.3:33825399-33825418 | MS.gene067914:CDS | 55.0% |
| ! | GATTGTGTGGGTGATGGTGC+AGG | + | chr8.3:33825537-33825556 | MS.gene067914:intron | 55.0% |
| ! | GGCTCTAATAGCCCAGCATC+AGG | - | chr8.3:33825390-33825409 | None:intergenic | 55.0% |
| !! | ATTAGAGCCTGGTGCTGGTG+GGG | + | chr8.3:33825401-33825420 | MS.gene067914:CDS | 55.0% |
| AATAGCCCAGCATCAGGCCC+AGG | - | chr8.3:33825384-33825403 | None:intergenic | 60.0% | |
| ACATCTTCCCCACCAGCACC+AGG | - | chr8.3:33825411-33825430 | None:intergenic | 60.0% | |
| AGCTCCTGGCTGCTCTAGTC+CGG | + | chr8.3:33825353-33825372 | MS.gene067914:CDS | 60.0% | |
| GATGTGTTTGGGCTTGGGCC+TGG | + | chr8.3:33825426-33825445 | MS.gene067914:CDS | 60.0% | |
| GGGCTATTAGAGCCTGGTGC+TGG | + | chr8.3:33825396-33825415 | MS.gene067914:CDS | 60.0% | |
| GCAGAGGGTGACTCAGCTCC+TGG | + | chr8.3:33825339-33825358 | MS.gene067914:CDS | 65.0% | |
| GCTCCTGGCTGCTCTAGTCC+GGG | + | chr8.3:33825354-33825373 | MS.gene067914:CDS | 65.0% | |
| AGCCCAGCATCAGGCCCAGG+TGG | - | chr8.3:33825381-33825400 | None:intergenic | 70.0% | |
| GCCCACCTGGGCCTGATGCT+GGG | + | chr8.3:33825376-33825395 | MS.gene067914:CDS | 70.0% | |
| GCCCAGCATCAGGCCCAGGT+GGG | - | chr8.3:33825380-33825399 | None:intergenic | 70.0% | |
| GCTCTAGTCCGGGCCCACCT+GGG | + | chr8.3:33825364-33825383 | MS.gene067914:CDS | 70.0% | |
| TGCTCTAGTCCGGGCCCACC+TGG | + | chr8.3:33825363-33825382 | MS.gene067914:CDS | 70.0% | |
| GCATCAGGCCCAGGTGGGCC+CGG | - | chr8.3:33825375-33825394 | None:intergenic | 75.0% | |
| GGCCCACCTGGGCCTGATGC+TGG | + | chr8.3:33825375-33825394 | MS.gene067914:CDS | 75.0% | |
| ! | GGGCCCGGACTAGAGCAGCC+AGG | - | chr8.3:33825360-33825379 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 33825276 | 33825665 | 33825276 | ID=MS.gene067914 |
| chr8.3 | mRNA | 33825276 | 33825665 | 33825276 | ID=MS.gene067914.t1;Parent=MS.gene067914 |
| chr8.3 | exon | 33825276 | 33825489 | 33825276 | ID=MS.gene067914.t1.exon1;Parent=MS.gene067914.t1 |
| chr8.3 | CDS | 33825276 | 33825489 | 33825276 | ID=cds.MS.gene067914.t1;Parent=MS.gene067914.t1 |
| chr8.3 | exon | 33825583 | 33825665 | 33825583 | ID=MS.gene067914.t1.exon2;Parent=MS.gene067914.t1 |
| chr8.3 | CDS | 33825583 | 33825665 | 33825583 | ID=cds.MS.gene067914.t1;Parent=MS.gene067914.t1 |
| Gene Sequence |
| Protein sequence |
