Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055401.t1 | AFK43360.1 | 93.5 | 199 | 9 | 1 | 1 | 195 | 1 | 199 | 8.00E-67 | 263.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055401.t1 | Q96316 | 49.2 | 124 | 61 | 1 | 1 | 124 | 1 | 122 | 2.8e-28 | 126.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055401.t1 | I3SSW8 | 93.5 | 199 | 9 | 1 | 1 | 195 | 1 | 199 | 5.8e-67 | 263.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050363 | MS.gene055401 | 0.828141 | 1.06E-54 | -1.69E-46 |
| MS.gene055401 | MS.gene056310 | 0.85195 | 6.35E-61 | -1.69E-46 |
| MS.gene055401 | MS.gene056311 | 0.810952 | 8.93E-51 | -1.69E-46 |
| MS.gene055401 | MS.gene056313 | 0.841603 | 4.30E-58 | -1.69E-46 |
| MS.gene055401 | MS.gene059517 | -0.80455 | 2.05E-49 | -1.69E-46 |
| MS.gene055401 | MS.gene064764 | 0.848718 | 5.13E-60 | -1.69E-46 |
| MS.gene055401 | MS.gene065465 | 0.807555 | 4.77E-50 | -1.69E-46 |
| MS.gene055401 | MS.gene067914 | 0.800423 | 1.45E-48 | -1.69E-46 |
| MS.gene055401 | MS.gene07448 | 0.805904 | 1.07E-49 | -1.69E-46 |
| MS.gene055401 | MS.gene09193 | -0.800168 | 1.64E-48 | -1.69E-46 |
| MS.gene055401 | MS.gene32061 | 0.827085 | 1.90E-54 | -1.69E-46 |
| MS.gene055401 | MS.gene38094 | 0.821411 | 4.10E-53 | -1.69E-46 |
| MS.gene055401 | MS.gene38097 | 0.809938 | 1.48E-50 | -1.69E-46 |
| MS.gene055401 | MS.gene38172 | 0.817331 | 3.49E-52 | -1.69E-46 |
| MS.gene055401 | MS.gene38173 | 0.838186 | 3.34E-57 | -1.69E-46 |
| MS.gene055401 | MS.gene38174 | 0.849478 | 3.15E-60 | -1.69E-46 |
| MS.gene055401 | MS.gene44720 | 0.843008 | 1.82E-58 | -1.69E-46 |
| MS.gene055401 | MS.gene44721 | 0.821912 | 3.14E-53 | -1.69E-46 |
| MS.gene055401 | MS.gene44722 | 0.836836 | 7.41E-57 | -1.69E-46 |
| MS.gene055401 | MS.gene44723 | 0.815623 | 8.41E-52 | -1.69E-46 |
| MS.gene055401 | MS.gene49885 | 0.809753 | 1.62E-50 | -1.69E-46 |
| MS.gene055401 | MS.gene49886 | 0.826992 | 2.00E-54 | -1.69E-46 |
| MS.gene055401 | MS.gene49887 | 0.815024 | 1.14E-51 | -1.69E-46 |
| MS.gene055401 | MS.gene60548 | 0.843664 | 1.22E-58 | -1.69E-46 |
| MS.gene055401 | MS.gene66720 | 0.803492 | 3.40E-49 | -1.69E-46 |
| MS.gene055401 | MS.gene69394 | 0.830913 | 2.25E-55 | -1.69E-46 |
| MS.gene055401 | MS.gene70354 | 0.829344 | 5.42E-55 | -1.69E-46 |
| MS.gene055401 | MS.gene70420 | 0.838261 | 3.20E-57 | -1.69E-46 |
| MS.gene055401 | MS.gene71525 | 0.827226 | 1.76E-54 | -1.69E-46 |
| MS.gene055401 | MS.gene71614 | 0.830683 | 2.56E-55 | -1.69E-46 |
| MS.gene055401 | MS.gene76146 | 0.807076 | 6.03E-50 | -1.69E-46 |
| MS.gene055401 | MS.gene77471 | 0.824223 | 9.07E-54 | -1.69E-46 |
| MS.gene055401 | MS.gene80092 | 0.817304 | 3.53E-52 | -1.69E-46 |
| MS.gene055401 | MS.gene86921 | 0.850437 | 1.70E-60 | -1.69E-46 |
| MS.gene055401 | MS.gene88816 | 0.808364 | 3.21E-50 | -1.69E-46 |
| MS.gene055401 | MS.gene91309 | 0.802646 | 5.08E-49 | -1.69E-46 |
| MS.gene055401 | MS.gene91973 | 0.829533 | 4.88E-55 | -1.69E-46 |
| MS.gene055401 | MS.gene91976 | 0.830778 | 2.42E-55 | -1.69E-46 |
| MS.gene055401 | MS.gene91977 | 0.829784 | 4.24E-55 | -1.69E-46 |
| MS.gene055401 | MS.gene96692 | 0.866228 | 3.32E-65 | -1.69E-46 |
| MS.gene055401 | MS.gene99141 | 0.830836 | 2.35E-55 | -1.69E-46 |
| MS.gene055401 | MS.gene99142 | 0.99852 | 1.54E-267 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055401.t1 | MTR_8g007020 | 88.718 | 195 | 14 | 1 | 1 | 195 | 1 | 187 | 5.67e-111 | 315 |
| MS.gene055401.t1 | MTR_8g007035 | 87.940 | 199 | 20 | 1 | 1 | 195 | 1 | 199 | 9.34e-105 | 300 |
| MS.gene055401.t1 | MTR_3g099580 | 56.303 | 119 | 49 | 2 | 2 | 120 | 5 | 120 | 1.25e-36 | 126 |
| MS.gene055401.t1 | MTR_3g099570 | 48.201 | 139 | 65 | 3 | 2 | 136 | 5 | 140 | 2.68e-34 | 120 |
| MS.gene055401.t1 | MTR_3g099540 | 49.231 | 130 | 62 | 3 | 2 | 130 | 5 | 131 | 2.20e-33 | 116 |
| MS.gene055401.t1 | MTR_3g099680 | 51.261 | 119 | 54 | 4 | 4 | 120 | 6 | 122 | 9.79e-31 | 112 |
| MS.gene055401.t1 | MTR_2g088990 | 39.726 | 146 | 83 | 3 | 8 | 152 | 11 | 152 | 1.18e-29 | 109 |
| MS.gene055401.t1 | MTR_5g006040 | 36.774 | 155 | 87 | 4 | 9 | 160 | 11 | 157 | 2.12e-29 | 107 |
| MS.gene055401.t1 | MTR_1g104800 | 34.211 | 152 | 95 | 3 | 11 | 159 | 12 | 161 | 5.14e-28 | 104 |
| MS.gene055401.t1 | MTR_3g099980 | 46.377 | 138 | 58 | 5 | 4 | 127 | 6 | 141 | 1.53e-26 | 101 |
| MS.gene055401.t1 | MTR_1g090190 | 37.037 | 135 | 82 | 2 | 21 | 155 | 20 | 151 | 5.14e-26 | 99.0 |
| MS.gene055401.t1 | MTR_6g080660 | 40.164 | 122 | 68 | 2 | 6 | 124 | 7 | 126 | 1.48e-23 | 93.2 |
| MS.gene055401.t1 | MTR_6g023760 | 46.602 | 103 | 52 | 2 | 19 | 120 | 21 | 121 | 5.41e-23 | 90.9 |
| MS.gene055401.t1 | MTR_1g112700 | 36.306 | 157 | 90 | 6 | 2 | 152 | 9 | 161 | 2.06e-22 | 89.7 |
| MS.gene055401.t1 | MTR_4g114870 | 43.284 | 134 | 64 | 5 | 10 | 133 | 10 | 141 | 2.37e-22 | 92.8 |
| MS.gene055401.t1 | MTR_4g114870 | 46.078 | 102 | 48 | 3 | 24 | 120 | 191 | 290 | 1.30e-21 | 90.9 |
| MS.gene055401.t1 | MTR_7g086100 | 40.336 | 119 | 63 | 3 | 4 | 121 | 11 | 122 | 1.58e-21 | 88.6 |
| MS.gene055401.t1 | MTR_2g043730 | 41.667 | 120 | 62 | 5 | 3 | 119 | 9 | 123 | 1.31e-20 | 83.2 |
| MS.gene055401.t1 | MTR_7g086160 | 38.655 | 119 | 71 | 2 | 5 | 122 | 8 | 125 | 1.98e-20 | 84.0 |
| MS.gene055401.t1 | MTR_1g090420 | 29.878 | 164 | 109 | 3 | 5 | 164 | 11 | 172 | 3.21e-20 | 84.7 |
| MS.gene055401.t1 | MTR_7g086140 | 38.655 | 119 | 70 | 3 | 5 | 122 | 8 | 124 | 1.00e-19 | 82.0 |
| MS.gene055401.t1 | MTR_7g086190 | 38.655 | 119 | 70 | 3 | 5 | 122 | 8 | 124 | 1.00e-19 | 82.0 |
| MS.gene055401.t1 | MTR_6g083240 | 39.130 | 138 | 72 | 5 | 21 | 148 | 24 | 159 | 3.70e-19 | 80.9 |
| MS.gene055401.t1 | MTR_1g105130 | 36.937 | 111 | 68 | 2 | 12 | 121 | 13 | 122 | 4.80e-19 | 80.1 |
| MS.gene055401.t1 | MTR_1g105120 | 36.937 | 111 | 68 | 2 | 12 | 121 | 13 | 122 | 4.80e-19 | 80.1 |
| MS.gene055401.t1 | MTR_2g101300 | 36.029 | 136 | 75 | 4 | 21 | 147 | 24 | 156 | 1.11e-18 | 80.1 |
| MS.gene055401.t1 | MTR_4g078410 | 32.934 | 167 | 103 | 3 | 21 | 184 | 22 | 182 | 1.36e-18 | 79.7 |
| MS.gene055401.t1 | MTR_2g043790 | 45.098 | 102 | 50 | 4 | 19 | 119 | 2 | 98 | 1.54e-18 | 77.0 |
| MS.gene055401.t1 | MTR_7g086090 | 38.136 | 118 | 65 | 3 | 4 | 120 | 11 | 121 | 3.37e-18 | 79.7 |
| MS.gene055401.t1 | MTR_3g089005 | 37.500 | 120 | 69 | 4 | 1 | 119 | 10 | 124 | 4.07e-18 | 77.0 |
| MS.gene055401.t1 | MTR_6g013420 | 35.211 | 142 | 80 | 5 | 10 | 149 | 24 | 155 | 1.40e-17 | 77.0 |
| MS.gene055401.t1 | MTR_8g463180 | 37.594 | 133 | 72 | 6 | 6 | 130 | 5 | 134 | 1.51e-17 | 79.0 |
| MS.gene055401.t1 | MTR_3g105930 | 34.722 | 144 | 79 | 4 | 40 | 182 | 48 | 177 | 1.88e-17 | 76.6 |
| MS.gene055401.t1 | MTR_8g086360 | 30.256 | 195 | 118 | 6 | 1 | 190 | 1 | 182 | 3.58e-17 | 75.9 |
| MS.gene055401.t1 | MTR_1g014120 | 34.899 | 149 | 88 | 4 | 1 | 142 | 1 | 147 | 3.84e-17 | 75.9 |
| MS.gene055401.t1 | MTR_8g089110 | 38.793 | 116 | 63 | 5 | 5 | 119 | 11 | 119 | 3.92e-17 | 74.3 |
| MS.gene055401.t1 | MTR_0334s0010 | 37.190 | 121 | 66 | 4 | 10 | 122 | 9 | 127 | 5.53e-17 | 75.9 |
| MS.gene055401.t1 | MTR_3g089035 | 41.346 | 104 | 55 | 4 | 19 | 121 | 28 | 126 | 6.24e-17 | 73.9 |
| MS.gene055401.t1 | MTR_6g013200 | 31.250 | 160 | 101 | 5 | 1 | 153 | 10 | 167 | 1.09e-16 | 74.3 |
| MS.gene055401.t1 | MTR_2g079030 | 34.591 | 159 | 97 | 4 | 6 | 159 | 7 | 163 | 1.90e-16 | 77.0 |
| MS.gene055401.t1 | MTR_4g007250 | 38.614 | 101 | 58 | 3 | 21 | 120 | 26 | 123 | 3.91e-16 | 72.8 |
| MS.gene055401.t1 | MTR_7g086220 | 33.333 | 123 | 79 | 3 | 1 | 122 | 3 | 123 | 4.16e-16 | 72.4 |
| MS.gene055401.t1 | MTR_7g086280 | 35.593 | 118 | 68 | 3 | 4 | 120 | 11 | 121 | 4.61e-16 | 72.8 |
| MS.gene055401.t1 | MTR_2g090580 | 34.254 | 181 | 96 | 7 | 21 | 184 | 24 | 198 | 7.60e-16 | 72.8 |
| MS.gene055401.t1 | MTR_4g130780 | 33.607 | 122 | 73 | 4 | 5 | 120 | 10 | 129 | 9.93e-16 | 72.0 |
| MS.gene055401.t1 | MTR_7g086230 | 36.893 | 103 | 62 | 2 | 19 | 120 | 21 | 121 | 1.63e-15 | 71.2 |
| MS.gene055401.t1 | MTR_4g067200 | 38.393 | 112 | 58 | 5 | 21 | 124 | 25 | 133 | 1.09e-14 | 68.9 |
| MS.gene055401.t1 | MTR_4g066110 | 38.393 | 112 | 58 | 5 | 21 | 124 | 25 | 133 | 1.09e-14 | 68.9 |
| MS.gene055401.t1 | MTR_6g022170 | 33.884 | 121 | 72 | 3 | 21 | 136 | 31 | 148 | 2.21e-14 | 68.2 |
| MS.gene055401.t1 | MTR_4g130800 | 33.600 | 125 | 75 | 4 | 5 | 123 | 10 | 132 | 5.13e-14 | 68.9 |
| MS.gene055401.t1 | MTR_4g111640 | 30.323 | 155 | 94 | 5 | 8 | 151 | 11 | 162 | 9.51e-14 | 66.6 |
| MS.gene055401.t1 | MTR_4g081100 | 30.622 | 209 | 114 | 6 | 8 | 191 | 16 | 218 | 1.05e-13 | 67.4 |
| MS.gene055401.t1 | MTR_2g025580 | 33.333 | 126 | 77 | 4 | 25 | 148 | 28 | 148 | 1.20e-13 | 66.2 |
| MS.gene055401.t1 | MTR_4g114830 | 38.144 | 97 | 52 | 5 | 21 | 109 | 25 | 121 | 3.22e-13 | 64.7 |
| MS.gene055401.t1 | MTR_2g090575 | 32.143 | 168 | 98 | 7 | 5 | 164 | 8 | 167 | 4.20e-13 | 67.0 |
| MS.gene055401.t1 | MTR_8g099220 | 39.048 | 105 | 56 | 4 | 21 | 120 | 26 | 127 | 5.01e-13 | 63.5 |
| MS.gene055401.t1 | MTR_4g077787 | 30.714 | 140 | 88 | 3 | 21 | 152 | 29 | 167 | 6.33e-13 | 64.7 |
| MS.gene055401.t1 | MTR_8g088830 | 37.624 | 101 | 56 | 5 | 21 | 119 | 25 | 120 | 1.25e-12 | 62.4 |
| MS.gene055401.t1 | MTR_8g095013 | 33.929 | 168 | 98 | 6 | 5 | 163 | 8 | 171 | 2.07e-12 | 64.7 |
| MS.gene055401.t1 | MTR_6g013170 | 33.594 | 128 | 76 | 5 | 26 | 152 | 33 | 152 | 5.73e-12 | 61.6 |
| MS.gene055401.t1 | MTR_4g130800 | 36.449 | 107 | 60 | 4 | 23 | 123 | 27 | 131 | 8.83e-12 | 62.8 |
| MS.gene055401.t1 | MTR_5g088170 | 35.294 | 119 | 68 | 6 | 27 | 142 | 20 | 132 | 1.27e-11 | 60.5 |
| MS.gene055401.t1 | MTR_8g095020 | 30.460 | 174 | 102 | 7 | 5 | 163 | 1 | 170 | 6.90e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055401.t1 | AT2G44790 | 53.704 | 108 | 46 | 1 | 17 | 124 | 25 | 128 | 6.56e-35 | 122 |
| MS.gene055401.t1 | AT3G60270 | 52.336 | 107 | 49 | 1 | 17 | 123 | 19 | 123 | 2.77e-34 | 120 |
| MS.gene055401.t1 | AT3G60280 | 53.271 | 107 | 48 | 1 | 17 | 123 | 17 | 121 | 8.26e-34 | 120 |
| MS.gene055401.t1 | AT2G32300 | 50.847 | 118 | 51 | 3 | 21 | 133 | 23 | 138 | 6.44e-33 | 119 |
| MS.gene055401.t1 | AT2G32300 | 45.270 | 148 | 68 | 4 | 21 | 167 | 53 | 188 | 1.43e-32 | 119 |
| MS.gene055401.t1 | AT1G72230 | 45.513 | 156 | 77 | 4 | 2 | 153 | 3 | 154 | 1.44e-31 | 113 |
| MS.gene055401.t1 | AT3G27200 | 41.803 | 122 | 68 | 2 | 1 | 121 | 3 | 122 | 7.72e-28 | 103 |
| MS.gene055401.t1 | AT5G07475 | 38.514 | 148 | 84 | 2 | 3 | 145 | 4 | 149 | 2.94e-27 | 102 |
| MS.gene055401.t1 | AT2G31050 | 42.336 | 137 | 70 | 4 | 8 | 137 | 8 | 142 | 2.15e-26 | 100 |
| MS.gene055401.t1 | AT2G26720 | 39.706 | 136 | 73 | 4 | 9 | 137 | 9 | 142 | 2.75e-25 | 97.8 |
| MS.gene055401.t1 | AT1G22480 | 47.154 | 123 | 63 | 1 | 32 | 154 | 27 | 147 | 3.02e-25 | 96.7 |
| MS.gene055401.t1 | AT5G26330 | 36.290 | 124 | 74 | 3 | 1 | 121 | 1 | 122 | 8.42e-24 | 93.2 |
| MS.gene055401.t1 | AT3G17675 | 39.604 | 101 | 58 | 2 | 21 | 120 | 29 | 127 | 2.83e-20 | 82.4 |
| MS.gene055401.t1 | AT5G53870 | 35.882 | 170 | 91 | 6 | 9 | 166 | 10 | 173 | 5.34e-20 | 86.7 |
| MS.gene055401.t1 | AT5G53870 | 35.882 | 170 | 91 | 6 | 9 | 166 | 10 | 173 | 7.24e-20 | 86.3 |
| MS.gene055401.t1 | AT2G25060 | 38.636 | 132 | 70 | 5 | 8 | 130 | 15 | 144 | 3.40e-19 | 81.3 |
| MS.gene055401.t1 | AT5G20230 | 36.364 | 176 | 98 | 7 | 23 | 190 | 24 | 193 | 1.19e-18 | 80.1 |
| MS.gene055401.t1 | AT4G31840 | 29.851 | 201 | 101 | 9 | 2 | 193 | 3 | 172 | 2.24e-17 | 76.3 |
| MS.gene055401.t1 | AT5G15350 | 34.483 | 145 | 72 | 5 | 6 | 138 | 1 | 134 | 3.07e-17 | 75.9 |
| MS.gene055401.t1 | AT1G79800 | 36.885 | 122 | 69 | 3 | 23 | 139 | 33 | 151 | 4.33e-17 | 75.9 |
| MS.gene055401.t1 | AT2G02850 | 39.286 | 112 | 62 | 4 | 11 | 121 | 23 | 129 | 5.45e-17 | 73.9 |
| MS.gene055401.t1 | AT4G12880 | 37.500 | 128 | 69 | 6 | 1 | 120 | 1 | 125 | 1.16e-16 | 73.6 |
| MS.gene055401.t1 | AT4G28365 | 32.727 | 165 | 98 | 7 | 9 | 164 | 12 | 172 | 5.27e-16 | 73.2 |
| MS.gene055401.t1 | AT4G27520 | 35.246 | 122 | 70 | 4 | 9 | 123 | 12 | 131 | 5.97e-16 | 75.1 |
| MS.gene055401.t1 | AT4G30590 | 44.578 | 83 | 43 | 2 | 41 | 122 | 50 | 130 | 1.11e-15 | 72.0 |
| MS.gene055401.t1 | AT1G64640 | 36.449 | 107 | 64 | 2 | 21 | 123 | 30 | 136 | 1.58e-15 | 71.6 |
| MS.gene055401.t1 | AT5G57920 | 33.333 | 99 | 63 | 2 | 39 | 136 | 43 | 139 | 6.23e-15 | 70.1 |
| MS.gene055401.t1 | AT5G57920 | 33.673 | 98 | 62 | 2 | 40 | 136 | 76 | 171 | 9.20e-15 | 70.1 |
| MS.gene055401.t1 | AT5G57920 | 33.333 | 99 | 63 | 2 | 39 | 136 | 42 | 138 | 1.19e-14 | 69.3 |
| MS.gene055401.t1 | AT3G20570 | 33.594 | 128 | 77 | 4 | 1 | 122 | 5 | 130 | 3.65e-14 | 68.2 |
| MS.gene055401.t1 | AT3G53330 | 34.400 | 125 | 76 | 4 | 20 | 140 | 184 | 306 | 1.68e-13 | 67.8 |
| MS.gene055401.t1 | AT2G27035 | 31.783 | 129 | 74 | 5 | 27 | 153 | 32 | 148 | 2.81e-12 | 62.4 |
| MS.gene055401.t1 | AT1G48940 | 33.058 | 121 | 72 | 4 | 5 | 118 | 7 | 125 | 5.38e-12 | 62.0 |
| MS.gene055401.t1 | AT3G18590 | 33.333 | 123 | 73 | 3 | 5 | 121 | 8 | 127 | 1.29e-11 | 61.2 |
| MS.gene055401.t1 | AT5G25090 | 27.907 | 129 | 84 | 4 | 2 | 123 | 3 | 129 | 2.94e-11 | 60.1 |
| MS.gene055401.t1 | AT4G32490 | 27.485 | 171 | 99 | 5 | 5 | 152 | 12 | 180 | 5.63e-11 | 60.1 |
Find 43 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGTTTCAGCAATGATTT+TGG | 0.095923 | 8.1:-86627689 | MS.gene055401:CDS |
| TCCATCAGCATAGTTCTTTA+TGG | 0.149502 | 8.1:+86628005 | None:intergenic |
| TTCTTTCTTTGTTCTATTGT+TGG | 0.249783 | 8.1:-86628453 | MS.gene055401:CDS |
| GATTACACCAAATGGGCTTC+TGG | 0.335655 | 8.1:-86628365 | MS.gene055401:CDS |
| CCATCACCACCAAAGGATAA+TGG | 0.368895 | 8.1:-86627757 | MS.gene055401:CDS |
| CTATGCTGATGGAAATTCTA+AGG | 0.389114 | 8.1:-86627995 | MS.gene055401:CDS |
| CCATTATCCTTTGGTGGTGA+TGG | 0.426718 | 8.1:+86627757 | None:intergenic |
| AAGGAGTTGATTACACCAAA+TGG | 0.428315 | 8.1:-86628373 | MS.gene055401:CDS |
| GGAGCTGATGGTGTGGTTGA+TGG | 0.434928 | 8.1:+86627802 | None:intergenic |
| GGTGCCACTTGGAGTTGGGG+TGG | 0.440747 | 8.1:+86627867 | None:intergenic |
| CTAACAGCTCCATTATCCTT+TGG | 0.447592 | 8.1:+86627748 | None:intergenic |
| TTCAAAGTTGGTGATAACTT+AGG | 0.466246 | 8.1:-86628335 | MS.gene055401:intron |
| GTAGTTGATGGTGTGGTTGA+TGG | 0.466465 | 8.1:+86627826 | None:intergenic |
| GTGGTTGATGGAGTAGTTGA+TGG | 0.471972 | 8.1:+86627814 | None:intergenic |
| AAGGTTGCATTGACTAAAGC+AGG | 0.472415 | 8.1:-86627976 | MS.gene055401:CDS |
| AAATCAGTTGCAAATGCATA+AGG | 0.473584 | 8.1:+86628425 | None:intergenic |
| CCAGGTCATTGTACTAGTAC+TGG | 0.496658 | 8.1:-86627928 | MS.gene055401:CDS |
| CATCAACTTCATCCACTTGG+TGG | 0.497098 | 8.1:+86628061 | None:intergenic |
| GAAGTTGAGTTTGTCTCTGA+AGG | 0.503772 | 8.1:+86627781 | None:intergenic |
| AGGAGTTGATTACACCAAAT+GGG | 0.504844 | 8.1:-86628372 | MS.gene055401:CDS |
| TACTTCATATGCCCTACTCC+AGG | 0.512113 | 8.1:-86627946 | MS.gene055401:CDS |
| AACTCCTTGATTCCATCCAC+TGG | 0.514824 | 8.1:+86628388 | None:intergenic |
| TTTGCAACTGATTTCACTGT+TGG | 0.522709 | 8.1:-86628416 | MS.gene055401:CDS |
| ACAGCTCCATTATCCTTTGG+TGG | 0.526576 | 8.1:+86627751 | None:intergenic |
| CTAGTACAATGACCTGGAGT+AGG | 0.540305 | 8.1:+86627934 | None:intergenic |
| TTTGTCTCTGAAGGAGCTGA+TGG | 0.541698 | 8.1:+86627790 | None:intergenic |
| CATCAAAAGACTAACACCAT+TGG | 0.545497 | 8.1:+86627720 | None:intergenic |
| CTCTGAAGGAGCTGATGGTG+TGG | 0.549422 | 8.1:+86627795 | None:intergenic |
| TTCACTGTTGGTGATGCCAG+TGG | 0.555149 | 8.1:-86628404 | MS.gene055401:CDS |
| GCCATAAAGAACTATGCTGA+TGG | 0.555563 | 8.1:-86628006 | MS.gene055401:CDS |
| TTTCATCAACTTCATCCACT+TGG | 0.560272 | 8.1:+86628058 | None:intergenic |
| AACTTCATCCACTTGGTGGA+AGG | 0.561488 | 8.1:+86628065 | None:intergenic |
| AACTTCTCCATCACCACCAA+AGG | 0.566009 | 8.1:-86627764 | MS.gene055401:CDS |
| TGATGGAGTAGTTGATGGTG+TGG | 0.574696 | 8.1:+86627819 | None:intergenic |
| GATGCCAGTGGATGGAATCA+AGG | 0.575971 | 8.1:-86628392 | MS.gene055401:CDS |
| CTGTTGGTGATGCCAGTGGA+TGG | 0.591217 | 8.1:-86628400 | MS.gene055401:CDS |
| TAGTACAATGACCTGGAGTA+GGG | 0.606340 | 8.1:+86627935 | None:intergenic |
| AGCACCACCCCAACTCCAAG+TGG | 0.615986 | 8.1:-86627871 | MS.gene055401:CDS |
| AGTTGCAAATGCATAAGGAA+AGG | 0.617334 | 8.1:+86628430 | None:intergenic |
| CCAGTACTAGTACAATGACC+TGG | 0.623789 | 8.1:+86627928 | None:intergenic |
| ATATGGCTCCTTCCACCAAG+TGG | 0.625567 | 8.1:-86628073 | MS.gene055401:CDS |
| GGTCATTGTACTAGTACTGG+TGG | 0.645353 | 8.1:-86627925 | MS.gene055401:CDS |
| GGAGCTGTTAGTGTTTCCAA+TGG | 0.664368 | 8.1:-86627736 | MS.gene055401:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTATGTTTTTTCTTATT+TGG | - | chr8.1:86627903-86627922 | MS.gene055401:CDS | 10.0% |
| !! | TATTTCAGTATATAAACGAA+AGG | + | chr8.1:86627880-86627899 | None:intergenic | 20.0% |
| ! | GAAAAAACAGTGTTCAAATA+TGG | - | chr8.1:86628058-86628077 | MS.gene055401:CDS | 25.0% |
| ! | TTCTTTCTTTGTTCTATTGT+TGG | - | chr8.1:86627695-86627714 | MS.gene055401:CDS | 25.0% |
| !! | TTTGTTTCAGCAATGATTTT+GGG | - | chr8.1:86628460-86628479 | MS.gene055401:CDS | 25.0% |
| !!! | GTTTGATAATGCTAAATTTG+AGG | - | chr8.1:86627939-86627958 | MS.gene055401:CDS | 25.0% |
| AAATCAGTTGCAAATGCATA+AGG | + | chr8.1:86627726-86627745 | None:intergenic | 30.0% | |
| TAGGTATGTATACATTCTGA+CGG | - | chr8.1:86627832-86627851 | MS.gene055401:CDS | 30.0% | |
| TCTGGAAAAACTTTCAAAGT+TGG | - | chr8.1:86627801-86627820 | MS.gene055401:CDS | 30.0% | |
| !! | CTTTGTTTCAGCAATGATTT+TGG | - | chr8.1:86628459-86628478 | MS.gene055401:CDS | 30.0% |
| !! | TTCAAAGTTGGTGATAACTT+AGG | - | chr8.1:86627813-86627832 | MS.gene055401:CDS | 30.0% |
| !!! | AATGGTGTTAGTCTTTTGAT+GGG | - | chr8.1:86628430-86628449 | MS.gene055401:CDS | 30.0% |
| AGTTGCAAATGCATAAGGAA+AGG | + | chr8.1:86627721-86627740 | None:intergenic | 35.0% | |
| CATCAAAAGACTAACACCAT+TGG | + | chr8.1:86628431-86628450 | None:intergenic | 35.0% | |
| CTATGCTGATGGAAATTCTA+AGG | - | chr8.1:86628153-86628172 | MS.gene055401:intron | 35.0% | |
| TCCATCAGCATAGTTCTTTA+TGG | + | chr8.1:86628146-86628165 | None:intergenic | 35.0% | |
| TTTCATCAACTTCATCCACT+TGG | + | chr8.1:86628093-86628112 | None:intergenic | 35.0% | |
| ! | AAGGAGTTGATTACACCAAA+TGG | - | chr8.1:86627775-86627794 | MS.gene055401:CDS | 35.0% |
| ! | AGGAGTTGATTACACCAAAT+GGG | - | chr8.1:86627776-86627795 | MS.gene055401:CDS | 35.0% |
| ! | TTGATGGAGATTTTGTTGGT+GGG | + | chr8.1:86628309-86628328 | None:intergenic | 35.0% |
| ! | TTTGCAACTGATTTCACTGT+TGG | - | chr8.1:86627732-86627751 | MS.gene055401:CDS | 35.0% |
| !!! | AGCAATGATTTTGGGTTTGA+TGG | - | chr8.1:86628468-86628487 | MS.gene055401:CDS | 35.0% |
| !!! | CAATGGTGTTAGTCTTTTGA+TGG | - | chr8.1:86628429-86628448 | MS.gene055401:CDS | 35.0% |
| !!! | GCAATGATTTTGGGTTTGAT+GGG | - | chr8.1:86628469-86628488 | MS.gene055401:CDS | 35.0% |
| CTAACAGCTCCATTATCCTT+TGG | + | chr8.1:86628403-86628422 | None:intergenic | 40.0% | |
| GAAGTTGAGTTTGTCTCTGA+AGG | + | chr8.1:86628370-86628389 | None:intergenic | 40.0% | |
| GCCATAAAGAACTATGCTGA+TGG | - | chr8.1:86628142-86628161 | MS.gene055401:intron | 40.0% | |
| TAGTACAATGACCTGGAGTA+GGG | + | chr8.1:86628216-86628235 | None:intergenic | 40.0% | |
| ! | AGTTTTTCCAGAAGCCCATT+TGG | + | chr8.1:86627793-86627812 | None:intergenic | 40.0% |
| ! | TGATGGAGATTTTGTTGGTG+GGG | + | chr8.1:86628308-86628327 | None:intergenic | 40.0% |
| !! | AAGGTTGCATTGACTAAAGC+AGG | - | chr8.1:86628172-86628191 | MS.gene055401:intron | 40.0% |
| !! | GTTGATGGAGATTTTGTTGG+TGG | + | chr8.1:86628310-86628329 | None:intergenic | 40.0% |
| !!! | GTGGTTGATGGAGATTTTGT+TGG | + | chr8.1:86628313-86628332 | None:intergenic | 40.0% |
| AACTCCTTGATTCCATCCAC+TGG | + | chr8.1:86627763-86627782 | None:intergenic | 45.0% | |
| AACTTCTCCATCACCACCAA+AGG | - | chr8.1:86628384-86628403 | MS.gene055401:CDS | 45.0% | |
| ACAGCTCCATTATCCTTTGG+TGG | + | chr8.1:86628400-86628419 | None:intergenic | 45.0% | |
| CATCAACTTCATCCACTTGG+TGG | + | chr8.1:86628090-86628109 | None:intergenic | 45.0% | |
| CCAGGTCATTGTACTAGTAC+TGG | - | chr8.1:86628220-86628239 | MS.gene055401:intron | 45.0% | |
| CCAGTACTAGTACAATGACC+TGG | + | chr8.1:86628223-86628242 | None:intergenic | 45.0% | |
| CCATCACCACCAAAGGATAA+TGG | - | chr8.1:86628391-86628410 | MS.gene055401:CDS | 45.0% | |
| CTAGTACAATGACCTGGAGT+AGG | + | chr8.1:86628217-86628236 | None:intergenic | 45.0% | |
| GGAGCTGTTAGTGTTTCCAA+TGG | - | chr8.1:86628412-86628431 | MS.gene055401:CDS | 45.0% | |
| TACTTCATATGCCCTACTCC+AGG | - | chr8.1:86628202-86628221 | MS.gene055401:intron | 45.0% | |
| ! | AACTTCATCCACTTGGTGGA+AGG | + | chr8.1:86628086-86628105 | None:intergenic | 45.0% |
| ! | GATTACACCAAATGGGCTTC+TGG | - | chr8.1:86627783-86627802 | MS.gene055401:CDS | 45.0% |
| ! | GGTCATTGTACTAGTACTGG+TGG | - | chr8.1:86628223-86628242 | MS.gene055401:intron | 45.0% |
| ! | TTTGTCTCTGAAGGAGCTGA+TGG | + | chr8.1:86628361-86628380 | None:intergenic | 45.0% |
| !! | ATGGAGATTTTGTTGGTGGG+GGG | + | chr8.1:86628306-86628325 | None:intergenic | 45.0% |
| !! | CCATTATCCTTTGGTGGTGA+TGG | + | chr8.1:86628394-86628413 | None:intergenic | 45.0% |
| !! | GATGGAGATTTTGTTGGTGG+GGG | + | chr8.1:86628307-86628326 | None:intergenic | 45.0% |
| !! | GTAGTTGATGGTGTGGTTGA+TGG | + | chr8.1:86628325-86628344 | None:intergenic | 45.0% |
| !! | GTGGTTGATGGAGTAGTTGA+TGG | + | chr8.1:86628337-86628356 | None:intergenic | 45.0% |
| !! | TGATGGAGTAGTTGATGGTG+TGG | + | chr8.1:86628332-86628351 | None:intergenic | 45.0% |
| ATATGGCTCCTTCCACCAAG+TGG | - | chr8.1:86628075-86628094 | MS.gene055401:CDS | 50.0% | |
| GATGCCAGTGGATGGAATCA+AGG | - | chr8.1:86627756-86627775 | MS.gene055401:CDS | 50.0% | |
| !! | TGGAGATTTTGTTGGTGGGG+GGG | + | chr8.1:86628305-86628324 | None:intergenic | 50.0% |
| !! | TTCACTGTTGGTGATGCCAG+TGG | - | chr8.1:86627744-86627763 | MS.gene055401:CDS | 50.0% |
| ! | CTCTGAAGGAGCTGATGGTG+TGG | + | chr8.1:86628356-86628375 | None:intergenic | 55.0% |
| ! | GGAGCTGATGGTGTGGTTGA+TGG | + | chr8.1:86628349-86628368 | None:intergenic | 55.0% |
| !! | CTGTTGGTGATGCCAGTGGA+TGG | - | chr8.1:86627748-86627767 | MS.gene055401:CDS | 55.0% |
| AGCACCACCCCAACTCCAAG+TGG | - | chr8.1:86628277-86628296 | MS.gene055401:intron | 60.0% | |
| !! | GGGGGGTGCCACTTGGAGTT+GGG | + | chr8.1:86628288-86628307 | None:intergenic | 65.0% |
| !! | GGGGGTGCCACTTGGAGTTG+GGG | + | chr8.1:86628287-86628306 | None:intergenic | 65.0% |
| !! | GGTGCCACTTGGAGTTGGGG+TGG | + | chr8.1:86628284-86628303 | None:intergenic | 65.0% |
| !! | GGGGGGGTGCCACTTGGAGT+TGG | + | chr8.1:86628289-86628308 | None:intergenic | 70.0% |
| !! | GTTGGTGGGGGGGTGCCACT+TGG | + | chr8.1:86628295-86628314 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 86627675 | 86628495 | 86627675 | ID=MS.gene055401 |
| chr8.1 | mRNA | 86627675 | 86628495 | 86627675 | ID=MS.gene055401.t1;Parent=MS.gene055401 |
| chr8.1 | exon | 86628336 | 86628495 | 86628336 | ID=MS.gene055401.t1.exon1;Parent=MS.gene055401.t1 |
| chr8.1 | CDS | 86628336 | 86628495 | 86628336 | ID=cds.MS.gene055401.t1;Parent=MS.gene055401.t1 |
| chr8.1 | exon | 86627675 | 86628102 | 86627675 | ID=MS.gene055401.t1.exon2;Parent=MS.gene055401.t1 |
| chr8.1 | CDS | 86627675 | 86628102 | 86627675 | ID=cds.MS.gene055401.t1;Parent=MS.gene055401.t1 |
| Gene Sequence |
| Protein sequence |
