Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068546.t1 | XP_013468304.1 | 97.8 | 270 | 6 | 0 | 8 | 277 | 1 | 270 | 1.70E-142 | 515.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068546.t1 | Q0WQJ7 | 75.1 | 269 | 59 | 4 | 10 | 277 | 2 | 263 | 1.6e-106 | 387.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068546.t1 | A0A072VL62 | 97.8 | 270 | 6 | 0 | 8 | 277 | 1 | 270 | 1.2e-142 | 515.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055578 | MS.gene068546 | 0.800172 | 1.63E-48 | -1.69E-46 |
| MS.gene056041 | MS.gene068546 | 0.820348 | 7.19E-53 | -1.69E-46 |
| MS.gene05640 | MS.gene068546 | 0.802378 | 5.77E-49 | -1.69E-46 |
| MS.gene06093 | MS.gene068546 | 0.806192 | 9.27E-50 | -1.69E-46 |
| MS.gene061260 | MS.gene068546 | 0.83015 | 3.45E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 60 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTGTTCTTCATCGCTTAA+TGG | 0.149663 | 1.1:-49896437 | None:intergenic |
| AACTGCACTGTCTTTGCTTA+TGG | 0.203169 | 1.1:+49896743 | MS.gene068546:CDS |
| TGTCGTGTAATGAAAATAAA+AGG | 0.235844 | 1.1:+49896480 | MS.gene068546:CDS |
| ATTGATCTCTGTTGTGATTT+CGG | 0.296531 | 1.1:-49896671 | None:intergenic |
| TGAATCGGGACGACGGCGTT+CGG | 0.323394 | 1.1:-49896244 | None:intergenic |
| TCTTTGTTATCCTGACGGTT+AGG | 0.329799 | 1.1:-49896285 | None:intergenic |
| CCTACTTTCTTAGACTGATC+TGG | 0.346314 | 1.1:-49896174 | None:intergenic |
| GAAGAAACCTGTAGCCCTTA+TGG | 0.347923 | 1.1:+49897110 | MS.gene068546:CDS |
| AACTTTCTCTACTTGTTTGT+TGG | 0.348646 | 1.1:-49896528 | None:intergenic |
| CAGTCTAAGAAAGTAGGGTT+AGG | 0.355683 | 1.1:+49896180 | MS.gene068546:CDS |
| TTCTTCACTCTTTCTTCAAT+AGG | 0.359638 | 1.1:-49897093 | None:intergenic |
| GAATCGGGACGACGGCGTTC+GGG | 0.361733 | 1.1:-49896243 | None:intergenic |
| GGCCTGGTAGAACTGTCTTC+TGG | 0.373427 | 1.1:-49897072 | None:intergenic |
| CGAGAACTTCATTGACAATT+GGG | 0.374555 | 1.1:-49896712 | None:intergenic |
| GGAGGGTGGGATGAGAAATA+AGG | 0.378900 | 1.1:+49896796 | MS.gene068546:CDS |
| TCGAGAACTTCATTGACAAT+TGG | 0.379235 | 1.1:-49896713 | None:intergenic |
| CTTTGTTATCCTGACGGTTA+GGG | 0.391090 | 1.1:-49896284 | None:intergenic |
| CCATCTTCCATAAGGGCTAC+AGG | 0.404889 | 1.1:-49897117 | None:intergenic |
| CCGATCAGAGCCTCGTGAAT+CGG | 0.406849 | 1.1:-49896259 | None:intergenic |
| CACTCTTTCTTCAATAGGCC+TGG | 0.432300 | 1.1:-49897088 | None:intergenic |
| CGATCAGAGCCTCGTGAATC+GGG | 0.450247 | 1.1:-49896258 | None:intergenic |
| TGACAATTGGGGAAATTGCT+TGG | 0.459148 | 1.1:-49896700 | None:intergenic |
| CTACTTTCTTAGACTGATCT+GGG | 0.463811 | 1.1:-49896173 | None:intergenic |
| CCTGTAGCCCTTATGGAAGA+TGG | 0.471287 | 1.1:+49897117 | MS.gene068546:CDS |
| GTCGTGTAATGAAAATAAAA+GGG | 0.472693 | 1.1:+49896481 | MS.gene068546:CDS |
| GGACGACGGCGTTCGGGTCG+AGG | 0.473129 | 1.1:-49896237 | None:intergenic |
| TCATTACACGACACCACCTT+CGG | 0.475943 | 1.1:-49896470 | None:intergenic |
| AAAGGTAGTGTGTTGTTAAG+AGG | 0.477663 | 1.1:+49897141 | MS.gene068546:CDS |
| AAATGAAATCTATACTCTGT+TGG | 0.480457 | 1.1:+49897191 | MS.gene068546:CDS |
| GTCTTTGCTTATGGACAGAC+CGG | 0.486032 | 1.1:+49896752 | MS.gene068546:CDS |
| CTTTATCTTTGTTATCCTGA+CGG | 0.486717 | 1.1:-49896290 | None:intergenic |
| GCTTATGGACAGACCGGGAC+TGG | 0.491101 | 1.1:+49896758 | MS.gene068546:CDS |
| AAAATTTGACGAACTGCTCT+CGG | 0.492168 | 1.1:-49896961 | None:intergenic |
| CATTGCTTAACAAGAGAAGC+AGG | 0.496816 | 1.1:+49897247 | MS.gene068546:CDS |
| CCAGATCAGTCTAAGAAAGT+AGG | 0.498378 | 1.1:+49896174 | MS.gene068546:CDS |
| AAGAGGACTTGAAGAAGAAT+CGG | 0.500002 | 1.1:+49897158 | MS.gene068546:CDS |
| GGTGATTTAACTGCTGAGGC+TGG | 0.508047 | 1.1:+49896931 | MS.gene068546:CDS |
| AGTCTAAGAAAGTAGGGTTA+GGG | 0.523529 | 1.1:+49896181 | MS.gene068546:CDS |
| TGATCGGAACCCTAACCGTC+AGG | 0.523617 | 1.1:+49896275 | MS.gene068546:CDS |
| GCCCTTATGGAAGATGGAAA+AGG | 0.531692 | 1.1:+49897123 | MS.gene068546:CDS |
| CAGATCAGTCTAAGAAAGTA+GGG | 0.533619 | 1.1:+49896175 | MS.gene068546:CDS |
| GACTGGTAAAACTTATACGA+TGG | 0.551667 | 1.1:+49896775 | MS.gene068546:CDS |
| CTCCAGAAGACAGTTCTACC+AGG | 0.551796 | 1.1:+49897070 | MS.gene068546:CDS |
| TTGTCAATGAAGTTCTCGAC+GGG | 0.559114 | 1.1:+49896717 | MS.gene068546:CDS |
| CCGATTCACGAGGCTCTGAT+CGG | 0.575179 | 1.1:+49896259 | MS.gene068546:CDS |
| AAAACTTATACGATGGAGGG+TGG | 0.588812 | 1.1:+49896782 | MS.gene068546:CDS |
| TGGTAAAACTTATACGATGG+AGG | 0.605460 | 1.1:+49896778 | MS.gene068546:CDS |
| ACGATCGAATGTTCCGAAGG+TGG | 0.607596 | 1.1:+49896457 | MS.gene068546:CDS |
| AGCCTCGTGAATCGGGACGA+CGG | 0.613226 | 1.1:-49896251 | None:intergenic |
| CGCCGTCGTCCCGATTCACG+AGG | 0.616893 | 1.1:+49896249 | MS.gene068546:CDS |
| GGGTGGTGATTTAACTGCTG+AGG | 0.631701 | 1.1:+49896927 | MS.gene068546:intron |
| ATTGTCAATGAAGTTCTCGA+CGG | 0.631832 | 1.1:+49896716 | MS.gene068546:CDS |
| GGTAAAACTTATACGATGGA+GGG | 0.633376 | 1.1:+49896779 | MS.gene068546:CDS |
| AAACTTATACGATGGAGGGT+GGG | 0.638061 | 1.1:+49896783 | MS.gene068546:CDS |
| ATCTATACTCTGTTGGAACG+AGG | 0.638922 | 1.1:+49897198 | MS.gene068546:CDS |
| GAGAACTTCATTGACAATTG+GGG | 0.654278 | 1.1:-49896711 | None:intergenic |
| ACAACGATCGAATGTTCCGA+AGG | 0.660517 | 1.1:+49896454 | MS.gene068546:CDS |
| TCTTTGCTTATGGACAGACC+GGG | 0.682220 | 1.1:+49896753 | MS.gene068546:CDS |
| TGGAACGAGGAGCATCAAAG+AGG | 0.716495 | 1.1:+49897211 | MS.gene068546:CDS |
| TACTTTCTTAGACTGATCTG+GGG | 0.719246 | 1.1:-49896172 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCGTCAAATTTTTGATATTT+TGG | + | chr1.1:49896972-49896991 | MS.gene068546:CDS | 20.0% |
| ! | AAAGCTATCTTACTATGATA+CGG | + | chr1.1:49896579-49896598 | MS.gene068546:intron | 25.0% |
| ! | AAATGAAATCTATACTCTGT+TGG | + | chr1.1:49897191-49897210 | MS.gene068546:CDS | 25.0% |
| ! | CACCCTATATTTATAAATCA+CGG | - | chr1.1:49896912-49896931 | None:intergenic | 25.0% |
| ! | GTAACATAACATCATATAAC+CGG | - | chr1.1:49896865-49896884 | None:intergenic | 25.0% |
| ! | GTCGTGTAATGAAAATAAAA+GGG | + | chr1.1:49896481-49896500 | MS.gene068546:CDS | 25.0% |
| ! | TGCCGTGATTTATAAATATA+GGG | + | chr1.1:49896907-49896926 | MS.gene068546:intron | 25.0% |
| ! | TGTCGTGTAATGAAAATAAA+AGG | + | chr1.1:49896480-49896499 | MS.gene068546:CDS | 25.0% |
| ! | TTGCCGTGATTTATAAATAT+AGG | + | chr1.1:49896906-49896925 | MS.gene068546:intron | 25.0% |
| !!! | CAATTGTTTTTGAATCTGTT+TGG | + | chr1.1:49896635-49896654 | MS.gene068546:intron | 25.0% |
| !!! | GAAAGTTTTCAATTTTGACA+AGG | + | chr1.1:49896544-49896563 | MS.gene068546:CDS | 25.0% |
| AACTTTCTCTACTTGTTTGT+TGG | - | chr1.1:49896531-49896550 | None:intergenic | 30.0% | |
| ATTGATCTCTGTTGTGATTT+CGG | - | chr1.1:49896674-49896693 | None:intergenic | 30.0% | |
| CGTGATTTATAAATATAGGG+TGG | + | chr1.1:49896910-49896929 | MS.gene068546:intron | 30.0% | |
| TTCTTCACTCTTTCTTCAAT+AGG | - | chr1.1:49897096-49897115 | None:intergenic | 30.0% | |
| ! | AATCTGTTTGGTAAGGTTTT+CGG | + | chr1.1:49896647-49896666 | MS.gene068546:intron | 30.0% |
| ! | CTTTATCTTTGTTATCCTGA+CGG | - | chr1.1:49896293-49896312 | None:intergenic | 30.0% |
| ! | GTTTTTGAATCTGTTTGGTA+AGG | + | chr1.1:49896640-49896659 | MS.gene068546:intron | 30.0% |
| AAAATTTGACGAACTGCTCT+CGG | - | chr1.1:49896964-49896983 | None:intergenic | 35.0% | |
| AAGAGGACTTGAAGAAGAAT+CGG | + | chr1.1:49897158-49897177 | MS.gene068546:CDS | 35.0% | |
| ACATAACATCATATAACCGG+TGG | - | chr1.1:49896862-49896881 | None:intergenic | 35.0% | |
| AGTCTAAGAAAGTAGGGTTA+GGG | + | chr1.1:49896181-49896200 | MS.gene068546:CDS | 35.0% | |
| ATTGTCAATGAAGTTCTCGA+CGG | + | chr1.1:49896716-49896735 | MS.gene068546:CDS | 35.0% | |
| CAGATCAGTCTAAGAAAGTA+GGG | + | chr1.1:49896175-49896194 | MS.gene068546:CDS | 35.0% | |
| CGAGAACTTCATTGACAATT+GGG | - | chr1.1:49896715-49896734 | None:intergenic | 35.0% | |
| CTACTTTCTTAGACTGATCT+GGG | - | chr1.1:49896176-49896195 | None:intergenic | 35.0% | |
| GACTGGTAAAACTTATACGA+TGG | + | chr1.1:49896775-49896794 | MS.gene068546:CDS | 35.0% | |
| GAGAACTTCATTGACAATTG+GGG | - | chr1.1:49896714-49896733 | None:intergenic | 35.0% | |
| GGTAAAACTTATACGATGGA+GGG | + | chr1.1:49896779-49896798 | MS.gene068546:CDS | 35.0% | |
| TACTTTCTTAGACTGATCTG+GGG | - | chr1.1:49896175-49896194 | None:intergenic | 35.0% | |
| TCGAGAACTTCATTGACAAT+TGG | - | chr1.1:49896716-49896735 | None:intergenic | 35.0% | |
| TGGTAAAACTTATACGATGG+AGG | + | chr1.1:49896778-49896797 | MS.gene068546:CDS | 35.0% | |
| TTAAAAATGGAGCAGGAGAT+GGG | - | chr1.1:49896215-49896234 | None:intergenic | 35.0% | |
| ! | AAAGGTAGTGTGTTGTTAAG+AGG | + | chr1.1:49897141-49897160 | MS.gene068546:CDS | 35.0% |
| ! | ACCTTTTCCATCTTCCATAA+GGG | - | chr1.1:49897127-49897146 | None:intergenic | 35.0% |
| ! | TACCTTTTCCATCTTCCATA+AGG | - | chr1.1:49897128-49897147 | None:intergenic | 35.0% |
| AAAACTTATACGATGGAGGG+TGG | + | chr1.1:49896782-49896801 | MS.gene068546:CDS | 40.0% | |
| AAACTTATACGATGGAGGGT+GGG | + | chr1.1:49896783-49896802 | MS.gene068546:CDS | 40.0% | |
| AACATCATATAACCGGTGGA+AGG | - | chr1.1:49896858-49896877 | None:intergenic | 40.0% | |
| AACTGCACTGTCTTTGCTTA+TGG | + | chr1.1:49896743-49896762 | MS.gene068546:CDS | 40.0% | |
| ATCTATACTCTGTTGGAACG+AGG | + | chr1.1:49897198-49897217 | MS.gene068546:CDS | 40.0% | |
| CAGTCTAAGAAAGTAGGGTT+AGG | + | chr1.1:49896180-49896199 | MS.gene068546:CDS | 40.0% | |
| CATTGCTTAACAAGAGAAGC+AGG | + | chr1.1:49897247-49897266 | MS.gene068546:CDS | 40.0% | |
| CCAGATCAGTCTAAGAAAGT+AGG | + | chr1.1:49896174-49896193 | MS.gene068546:CDS | 40.0% | |
| CCTACTTTCTTAGACTGATC+TGG | - | chr1.1:49896177-49896196 | None:intergenic | 40.0% | |
| CGTTGTTCTTCATCGCTTAA+TGG | - | chr1.1:49896440-49896459 | None:intergenic | 40.0% | |
| GTTAAAAATGGAGCAGGAGA+TGG | - | chr1.1:49896216-49896235 | None:intergenic | 40.0% | |
| TGACAATTGGGGAAATTGCT+TGG | - | chr1.1:49896703-49896722 | None:intergenic | 40.0% | |
| TTGTCAATGAAGTTCTCGAC+GGG | + | chr1.1:49896717-49896736 | MS.gene068546:CDS | 40.0% | |
| ! | CGTATAAGTTTTACCAGTCC+CGG | - | chr1.1:49896774-49896793 | None:intergenic | 40.0% |
| ! | CTTTGTTATCCTGACGGTTA+GGG | - | chr1.1:49896287-49896306 | None:intergenic | 40.0% |
| ! | TCTTTGTTATCCTGACGGTT+AGG | - | chr1.1:49896288-49896307 | None:intergenic | 40.0% |
| !! | TTCAAGTTTTGCTTCGTTGC+AGG | + | chr1.1:49896322-49896341 | MS.gene068546:CDS | 40.0% |
| ACAACGATCGAATGTTCCGA+AGG | + | chr1.1:49896454-49896473 | MS.gene068546:CDS | 45.0% | |
| CACTCTTTCTTCAATAGGCC+TGG | - | chr1.1:49897091-49897110 | None:intergenic | 45.0% | |
| GAAGAAACCTGTAGCCCTTA+TGG | + | chr1.1:49897110-49897129 | MS.gene068546:CDS | 45.0% | |
| GCCCTTATGGAAGATGGAAA+AGG | + | chr1.1:49897123-49897142 | MS.gene068546:CDS | 45.0% | |
| GTCTTTGCTTATGGACAGAC+CGG | + | chr1.1:49896752-49896771 | MS.gene068546:CDS | 45.0% | |
| TCATTACACGACACCACCTT+CGG | - | chr1.1:49896473-49896492 | None:intergenic | 45.0% | |
| TCTTTGCTTATGGACAGACC+GGG | + | chr1.1:49896753-49896772 | MS.gene068546:CDS | 45.0% | |
| ! | CGAGGTGTTAAAAATGGAGC+AGG | - | chr1.1:49896222-49896241 | None:intergenic | 45.0% |
| ! | TCGGGTCGAGGTGTTAAAAA+TGG | - | chr1.1:49896228-49896247 | None:intergenic | 45.0% |
| !!! | TAATTTAATTTTCTAATTTT+AGG | + | chr1.1:49896414-49896433 | MS.gene068546:intron | 5.0% |
| ACGATCGAATGTTCCGAAGG+TGG | + | chr1.1:49896457-49896476 | MS.gene068546:CDS | 50.0% | |
| CCATCTTCCATAAGGGCTAC+AGG | - | chr1.1:49897120-49897139 | None:intergenic | 50.0% | |
| CCTGTAGCCCTTATGGAAGA+TGG | + | chr1.1:49897117-49897136 | MS.gene068546:CDS | 50.0% | |
| CGACTCACATTTCCTTCCAC+CGG | + | chr1.1:49896843-49896862 | MS.gene068546:intron | 50.0% | |
| CTCCAGAAGACAGTTCTACC+AGG | + | chr1.1:49897070-49897089 | MS.gene068546:CDS | 50.0% | |
| GGAGGGTGGGATGAGAAATA+AGG | + | chr1.1:49896796-49896815 | MS.gene068546:CDS | 50.0% | |
| GGGTGGTGATTTAACTGCTG+AGG | + | chr1.1:49896927-49896946 | MS.gene068546:intron | 50.0% | |
| GGTGATTTAACTGCTGAGGC+TGG | + | chr1.1:49896931-49896950 | MS.gene068546:CDS | 50.0% | |
| !! | TGGAACGAGGAGCATCAAAG+AGG | + | chr1.1:49897211-49897230 | MS.gene068546:CDS | 50.0% |
| CCGATCAGAGCCTCGTGAAT+CGG | - | chr1.1:49896262-49896281 | None:intergenic | 55.0% | |
| CGATCAGAGCCTCGTGAATC+GGG | - | chr1.1:49896261-49896280 | None:intergenic | 55.0% | |
| GGCCTGGTAGAACTGTCTTC+TGG | - | chr1.1:49897075-49897094 | None:intergenic | 55.0% | |
| TGATCGGAACCCTAACCGTC+AGG | + | chr1.1:49896275-49896294 | MS.gene068546:CDS | 55.0% | |
| ! | CCGATTCACGAGGCTCTGAT+CGG | + | chr1.1:49896259-49896278 | MS.gene068546:CDS | 55.0% |
| AGCCTCGTGAATCGGGACGA+CGG | - | chr1.1:49896254-49896273 | None:intergenic | 60.0% | |
| GCTTATGGACAGACCGGGAC+TGG | + | chr1.1:49896758-49896777 | MS.gene068546:CDS | 60.0% | |
| TGAATCGGGACGACGGCGTT+CGG | - | chr1.1:49896247-49896266 | None:intergenic | 60.0% | |
| GAATCGGGACGACGGCGTTC+GGG | - | chr1.1:49896246-49896265 | None:intergenic | 65.0% | |
| CGCCGTCGTCCCGATTCACG+AGG | + | chr1.1:49896249-49896268 | MS.gene068546:CDS | 70.0% | |
| GGACGACGGCGTTCGGGTCG+AGG | - | chr1.1:49896240-49896259 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 49896137 | 49897272 | 49896137 | ID=MS.gene068546 |
| chr1.1 | mRNA | 49896137 | 49897272 | 49896137 | ID=MS.gene068546.t1;Parent=MS.gene068546 |
| chr1.1 | exon | 49896137 | 49896343 | 49896137 | ID=MS.gene068546.t1.exon1;Parent=MS.gene068546.t1 |
| chr1.1 | CDS | 49896137 | 49896343 | 49896137 | ID=cds.MS.gene068546.t1;Parent=MS.gene068546.t1 |
| chr1.1 | exon | 49896436 | 49896565 | 49896436 | ID=MS.gene068546.t1.exon2;Parent=MS.gene068546.t1 |
| chr1.1 | CDS | 49896436 | 49896565 | 49896436 | ID=cds.MS.gene068546.t1;Parent=MS.gene068546.t1 |
| chr1.1 | exon | 49896662 | 49896817 | 49896662 | ID=MS.gene068546.t1.exon3;Parent=MS.gene068546.t1 |
| chr1.1 | CDS | 49896662 | 49896817 | 49896662 | ID=cds.MS.gene068546.t1;Parent=MS.gene068546.t1 |
| chr1.1 | exon | 49896928 | 49897272 | 49896928 | ID=MS.gene068546.t1.exon4;Parent=MS.gene068546.t1 |
| chr1.1 | CDS | 49896928 | 49897272 | 49896928 | ID=cds.MS.gene068546.t1;Parent=MS.gene068546.t1 |
| Gene Sequence |
| Protein sequence |
