AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068723

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068723.t1 MTR_2g078960 95.277 487 17 1 1 487 1 481 0.0 934
MS.gene068723.t1 MTR_2g078930 88.798 491 51 2 1 487 1 491 0.0 859
MS.gene068723.t1 MTR_2g079020 86.066 488 64 2 1 487 1 485 0.0 821
MS.gene068723.t1 MTR_2g078970 81.967 488 78 2 1 487 1 479 0.0 781
MS.gene068723.t1 MTR_2g078990 83.766 308 50 0 180 487 1 308 0.0 537
MS.gene068723.t1 MTR_2g079030 85.714 210 30 0 278 487 319 528 9.20e-130 387
MS.gene068723.t1 MTR_6g081780 42.733 461 248 4 18 472 10 460 3.28e-126 376
MS.gene068723.t1 MTR_1g108810 41.775 462 268 1 11 472 7 467 3.65e-126 376
MS.gene068723.t1 MTR_6g081410 41.983 474 252 6 18 483 10 468 5.37e-125 373
MS.gene068723.t1 MTR_8g106670 42.105 437 253 0 36 472 9 445 9.75e-121 362
MS.gene068723.t1 MTR_6g081240 42.431 436 249 2 38 472 9 443 3.17e-116 349
MS.gene068723.t1 MTR_1g108840 41.228 456 266 1 18 473 22 475 3.72e-115 348
MS.gene068723.t1 MTR_6g081400 39.013 446 268 2 39 483 15 457 4.66e-113 342
MS.gene068723.t1 MTR_6g081300 40.920 435 257 0 38 472 9 443 4.73e-112 339
MS.gene068723.t1 MTR_6g081810 38.266 473 258 3 11 483 4 442 1.08e-110 335
MS.gene068723.t1 MTR_6g066380 38.994 477 277 4 11 483 4 470 2.88e-109 333
MS.gene068723.t1 MTR_6g081390 38.789 446 269 2 39 483 12 454 1.64e-108 330
MS.gene068723.t1 MTR_4g048000 36.688 477 295 1 7 483 39 508 1.78e-104 322
MS.gene068723.t1 MTR_7g082810 38.717 452 277 0 21 472 2 453 2.31e-104 320
MS.gene068723.t1 MTR_6g081310 39.821 447 243 3 38 472 9 441 6.54e-103 316
MS.gene068723.t1 MTR_6g004220 38.022 455 279 2 21 472 3 457 1.09e-100 310
MS.gene068723.t1 MTR_6g004220 37.609 460 284 2 16 472 5 464 1.66e-100 311
MS.gene068723.t1 MTR_2g088400 34.064 502 309 2 3 482 2 503 4.84e-100 310
MS.gene068723.t1 MTR_1g108990 37.229 462 285 2 11 472 2 458 1.38e-96 300
MS.gene068723.t1 MTR_2g022980 34.286 490 305 3 8 483 6 492 3.97e-95 296
MS.gene068723.t1 MTR_2g088400 34.348 460 288 1 3 448 2 461 1.99e-92 288
MS.gene068723.t1 MTR_1g109060 36.446 439 269 2 39 472 24 457 2.41e-89 281
MS.gene068723.t1 MTR_1g109060 36.446 439 269 2 39 472 24 457 4.52e-89 281
MS.gene068723.t1 MTR_5g090290 35.874 446 285 1 25 470 33 477 1.76e-86 276
MS.gene068723.t1 MTR_7g082800 35.990 389 248 1 98 485 1 389 3.64e-82 260
MS.gene068723.t1 MTR_8g069470 34.023 435 286 1 38 472 49 482 3.45e-81 261
MS.gene068723.t1 MTR_8g063590 36.092 435 275 3 40 472 54 487 8.48e-81 260
MS.gene068723.t1 MTR_5g032720 33.888 481 304 5 10 483 13 486 1.10e-80 259
MS.gene068723.t1 MTR_1g101500 32.979 470 308 3 13 479 39 504 1.49e-80 260
MS.gene068723.t1 MTR_7g087400 37.868 441 258 4 39 472 30 461 3.44e-80 258
MS.gene068723.t1 MTR_6g004220 36.896 393 245 2 16 405 5 397 9.83e-80 255
MS.gene068723.t1 MTR_1g100180 35.828 441 278 3 33 472 38 474 1.30e-79 256
MS.gene068723.t1 MTR_5g033100 33.408 446 295 2 38 482 55 499 2.44e-78 254
MS.gene068723.t1 MTR_1g021740 32.766 470 309 3 3 472 5 467 1.28e-75 246
MS.gene068723.t1 MTR_5g010830 34.023 435 280 3 36 466 62 493 3.88e-75 246
MS.gene068723.t1 MTR_7g087410 34.793 434 282 1 39 472 31 463 6.36e-75 244
MS.gene068723.t1 MTR_5g087930 32.591 494 272 8 3 485 19 462 1.20e-73 240
MS.gene068723.t1 MTR_5g067460 31.070 486 312 6 15 485 35 512 5.78e-73 241
MS.gene068723.t1 MTR_0039s0080 31.250 448 295 3 48 485 47 491 1.74e-71 236
MS.gene068723.t1 MTR_6g047640 33.830 470 294 4 11 472 2 462 6.03e-71 233
MS.gene068723.t1 MTR_6g047610 32.759 464 306 4 11 472 2 461 1.22e-70 233
MS.gene068723.t1 MTR_5g032750 33.833 467 295 7 10 472 13 469 1.88e-70 232
MS.gene068723.t1 MTR_7g087370 34.934 458 252 6 22 472 14 432 2.62e-70 231
MS.gene068723.t1 MTR_7g087430 37.333 375 234 1 98 472 1 374 1.97e-69 227
MS.gene068723.t1 MTR_6g047640 32.553 470 281 5 11 472 2 443 2.66e-69 229
MS.gene068723.t1 MTR_7g087410 36.267 375 238 1 98 472 1 374 3.29e-68 224
MS.gene068723.t1 MTR_8g102430 32.604 457 279 5 15 470 6 434 6.47e-68 224
MS.gene068723.t1 MTR_6g027200 32.874 435 289 2 39 472 36 468 1.08e-67 225
MS.gene068723.t1 MTR_3g099700 32.009 453 305 3 21 472 29 479 1.82e-66 222
MS.gene068723.t1 MTR_3g111020 30.357 448 302 4 10 451 20 463 3.18e-66 223
MS.gene068723.t1 MTR_3g099720 30.917 469 311 3 21 483 29 490 3.37e-66 221
MS.gene068723.t1 MTR_6g047590 33.405 464 303 4 11 472 2 461 1.04e-64 217
MS.gene068723.t1 MTR_6g088805 30.983 468 298 6 18 472 2 457 3.97e-64 216
MS.gene068723.t1 MTR_6g004220 37.179 312 193 2 16 324 5 316 9.51e-64 210
MS.gene068723.t1 MTR_6g043810 30.837 454 299 4 38 479 22 472 9.66e-64 215
MS.gene068723.t1 MTR_6g043810 30.853 457 295 6 38 479 32 482 1.30e-63 215
MS.gene068723.t1 MTR_6g027190 33.103 435 288 2 39 472 24 456 1.43e-60 206
MS.gene068723.t1 MTR_5g011540 30.388 464 304 4 34 484 38 495 2.48e-60 206
MS.gene068723.t1 MTR_4g027920 30.806 422 284 4 39 455 156 574 1.93e-59 206
MS.gene068723.t1 MTR_4g051552 74.545 110 21 3 187 295 191 294 2.21e-48 169
MS.gene068723.t1 MTR_2g435930 24.729 461 325 2 38 479 18 475 1.85e-40 152
MS.gene068723.t1 MTR_5g090280 28.678 401 250 5 98 470 1 393 5.82e-39 146
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068723.t1 AT3G23560 61.359 471 179 1 9 476 5 475 0.0 594
MS.gene068723.t1 AT3G23550 61.255 462 179 0 13 474 4 465 0.0 585
MS.gene068723.t1 AT3G23560 61.000 400 153 1 9 405 5 404 3.55e-175 498
MS.gene068723.t1 AT5G52450 44.470 434 241 0 39 472 25 458 9.83e-117 352
MS.gene068723.t1 AT2G34360 42.291 454 246 4 20 472 15 453 1.67e-116 351
MS.gene068723.t1 AT1G73700 41.856 485 263 3 6 482 1 474 1.64e-114 346
MS.gene068723.t1 AT1G15150 39.565 460 272 3 17 470 2 461 7.94e-107 327
MS.gene068723.t1 AT2G04040 38.641 471 267 6 11 472 2 459 2.17e-103 317
MS.gene068723.t1 AT1G15160 38.166 469 284 3 17 479 2 470 2.00e-102 315
MS.gene068723.t1 AT1G15170 39.780 455 268 2 32 483 23 474 2.34e-102 315
MS.gene068723.t1 AT1G66760 37.446 462 282 1 11 472 6 460 4.64e-102 313
MS.gene068723.t1 AT1G66760 37.446 462 282 1 11 472 6 460 5.29e-102 314
MS.gene068723.t1 AT2G04070 37.660 470 273 5 11 472 2 459 2.70e-100 310
MS.gene068723.t1 AT2G04050 37.797 463 282 4 11 472 2 459 3.34e-98 304
MS.gene068723.t1 AT1G71140 37.802 455 270 4 23 472 12 458 3.57e-98 304
MS.gene068723.t1 AT1G11670 36.261 444 283 0 40 483 50 493 9.01e-98 304
MS.gene068723.t1 AT2G04080 35.714 476 297 5 11 485 2 469 3.22e-97 301
MS.gene068723.t1 AT2G04080 36.285 463 289 4 11 472 2 459 7.87e-97 300
MS.gene068723.t1 AT2G34360 37.528 453 227 2 20 472 15 411 1.32e-96 299
MS.gene068723.t1 AT2G04100 37.797 463 285 3 11 472 2 462 1.56e-96 300
MS.gene068723.t1 AT2G04100 38.065 465 281 4 11 472 2 462 1.87e-96 300
MS.gene068723.t1 AT3G21690 35.426 446 288 0 38 483 50 495 3.90e-95 297
MS.gene068723.t1 AT1G15180 39.765 425 253 1 32 453 24 448 1.62e-93 292
MS.gene068723.t1 AT1G61890 34.910 444 289 0 40 483 47 490 6.75e-93 291
MS.gene068723.t1 AT2G04090 36.853 464 288 4 11 472 2 462 2.44e-92 289
MS.gene068723.t1 AT1G61890 35.335 433 280 0 40 472 47 479 4.37e-92 289
MS.gene068723.t1 AT1G66780 35.697 423 271 1 59 481 54 475 6.01e-92 288
MS.gene068723.t1 AT4G21903 36.471 425 261 2 67 483 80 503 4.86e-90 284
MS.gene068723.t1 AT4G21903 37.192 406 254 1 67 472 80 484 1.77e-89 283
MS.gene068723.t1 AT4G21910 34.513 452 289 2 40 485 57 507 4.88e-89 281
MS.gene068723.t1 AT4G21910 34.513 452 289 2 40 485 57 507 4.88e-89 281
MS.gene068723.t1 AT3G59030 35.124 484 293 6 7 472 3 483 9.07e-89 281
MS.gene068723.t1 AT4G21910 34.071 452 291 2 40 485 55 505 3.45e-88 279
MS.gene068723.t1 AT4G21910 34.071 452 291 2 40 485 55 505 3.45e-88 279
MS.gene068723.t1 AT1G64820 33.703 451 290 3 36 483 25 469 1.88e-86 275
MS.gene068723.t1 AT1G47530 36.009 461 292 3 13 472 8 466 3.33e-85 271
MS.gene068723.t1 AT1G15170 40.517 348 204 1 136 483 11 355 6.19e-85 266
MS.gene068723.t1 AT1G61890 35.062 405 263 0 40 444 47 451 9.07e-84 266
MS.gene068723.t1 AT1G15150 41.493 335 196 0 136 470 11 345 1.18e-83 263
MS.gene068723.t1 AT1G23300 35.104 433 280 1 40 472 44 475 3.69e-83 266
MS.gene068723.t1 AT1G33110 35.808 458 286 4 16 472 17 467 7.79e-83 265
MS.gene068723.t1 AT4G21903 35.533 394 246 1 98 483 1 394 4.64e-82 259
MS.gene068723.t1 AT1G33090 36.518 471 291 4 17 486 18 481 6.49e-82 263
MS.gene068723.t1 AT1G33100 36.069 463 289 3 10 472 9 464 1.48e-81 261
MS.gene068723.t1 AT3G26590 33.941 439 278 4 50 482 55 487 5.99e-81 260
MS.gene068723.t1 AT1G66760 36.709 395 243 1 11 405 6 393 2.43e-80 255
MS.gene068723.t1 AT5G44050 34.672 473 298 5 3 472 8 472 5.80e-79 254
MS.gene068723.t1 AT1G12950 33.485 439 280 4 50 482 77 509 2.42e-78 254
MS.gene068723.t1 AT5G38030 32.952 437 291 2 34 470 40 474 2.29e-77 251
MS.gene068723.t1 AT1G47530 37.067 375 235 1 98 472 1 374 1.16e-75 243
MS.gene068723.t1 AT4G00350 34.199 462 296 4 14 472 62 518 9.43e-75 245
MS.gene068723.t1 AT3G26590 35.038 391 242 4 98 482 1 385 1.38e-74 240
MS.gene068723.t1 AT1G33080 33.906 466 298 3 10 472 9 467 6.44e-74 241
MS.gene068723.t1 AT5G10420 35.320 453 280 4 7 453 5 450 6.91e-74 241
MS.gene068723.t1 AT1G33080 33.906 466 298 3 10 472 9 467 8.01e-74 241
MS.gene068723.t1 AT5G65380 32.623 469 307 6 7 472 7 469 4.08e-70 231
MS.gene068723.t1 AT4G25640 33.045 463 305 3 12 472 5 464 3.62e-67 224
MS.gene068723.t1 AT4G25640 33.045 463 305 3 12 472 5 464 7.39e-67 224
MS.gene068723.t1 AT2G38510 30.973 452 305 3 39 483 5 456 7.76e-67 223
MS.gene068723.t1 AT1G66760 35.650 331 213 0 142 472 2 332 2.28e-66 217
MS.gene068723.t1 AT5G19700 30.238 463 318 2 18 479 17 475 9.57e-66 221
MS.gene068723.t1 AT4G29140 30.716 433 300 0 38 470 54 486 1.77e-65 220
MS.gene068723.t1 AT5G52050 29.325 474 317 4 6 470 7 471 1.34e-64 218
MS.gene068723.t1 AT1G58340 31.294 425 292 0 27 451 45 469 3.90e-64 217
MS.gene068723.t1 AT4G23030 33.333 489 302 8 12 483 3 484 3.96e-64 216
MS.gene068723.t1 AT4G25640 32.639 432 286 3 12 441 5 433 2.46e-62 210
MS.gene068723.t1 AT5G17700 33.410 434 284 5 39 470 33 463 1.28e-61 209
MS.gene068723.t1 AT5G17700 33.410 434 284 5 39 470 33 463 1.28e-61 209
MS.gene068723.t1 AT3G59030 32.134 417 262 6 7 405 3 416 4.07e-61 206
MS.gene068723.t1 AT5G49130 28.482 481 321 3 11 477 6 477 1.34e-60 207
MS.gene068723.t1 AT1G71870 30.000 460 294 4 11 451 2 452 7.37e-60 205
MS.gene068723.t1 AT3G03620 31.590 459 302 5 16 470 16 466 8.12e-60 204
MS.gene068723.t1 AT3G03620 31.590 459 302 5 16 470 16 466 8.12e-60 204
MS.gene068723.t1 AT1G33110 33.929 392 251 4 16 406 17 401 2.75e-59 201
MS.gene068723.t1 AT4G22790 30.979 439 297 4 38 472 28 464 1.68e-55 193
MS.gene068723.t1 AT1G15180 38.889 270 165 0 136 405 11 280 3.30e-55 190
MS.gene068723.t1 AT5G17700 32.520 369 244 5 39 405 33 398 1.57e-46 167
MS.gene068723.t1 AT2G04066 35.556 135 79 1 338 472 28 154 2.65e-21 91.3

Find 91 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAAACATGAGTTGAATTT+TGG 0.138353 2.3:+18953197 None:intergenic
GTGCTACTCTTGCTAATTCT+TGG 0.196457 2.3:-18953083 MS.gene068723:CDS
TTGTATTTGGCCATGATATA+TGG 0.227753 2.3:-18950255 MS.gene068723:CDS
CAAGGAGGAGAGAGAGCTTT+AGG 0.245659 2.3:+18950299 None:intergenic
CACAAGAGTTTCCAATGAAT+TGG 0.264220 2.3:-18950365 MS.gene068723:CDS
TAAGACCGATTTGCAGTATA+AGG 0.273484 2.3:-18949927 MS.gene068723:intron
GTCATGCTTGTTGGTCATCT+TGG 0.292545 2.3:-18953123 MS.gene068723:CDS
GAAGTTGTTATTTGTGAATC+AGG 0.295836 2.3:+18950807 None:intergenic
TTCCACCATTTCTCTATTTG+TGG 0.302774 2.3:+18953243 None:intergenic
TTATGCAAGTTTGGAGTATT+GGG 0.305683 2.3:-18950865 MS.gene068723:intron
AATAAATTTGTAAGAATCAT+TGG 0.316353 2.3:+18953171 None:intergenic
TCTGTCCAAGGTGTCTTATC+AGG 0.328815 2.3:-18950148 MS.gene068723:intron
CAACTTCATTGATTGCAATA+TGG 0.330876 2.3:-18950791 MS.gene068723:intron
TTAAGTTGTTAAACAACAAT+AGG 0.334821 2.3:+18949149 None:intergenic
TGTGGATTGGTGTGTCAAAC+TGG 0.344629 2.3:-18949245 MS.gene068723:CDS
ATTATGCAAGTTTGGAGTAT+TGG 0.350836 2.3:-18950866 MS.gene068723:intron
TTGGTTTCTGTCATGCTTGT+TGG 0.351093 2.3:-18953132 MS.gene068723:CDS
TGATAGTCCTATTATCAAAA+AGG 0.356312 2.3:-18950218 MS.gene068723:CDS
GTTCATGTTGGAATTGCATA+TGG 0.356912 2.3:-18951539 MS.gene068723:CDS
CAATCATTATATCAATTGTT+TGG 0.358665 2.3:-18951748 MS.gene068723:CDS
CTGCAACTTCTATTTCACTA+TGG 0.362518 2.3:-18951475 MS.gene068723:CDS
CTGTCTCAGGTTGGTTTAAG+TGG 0.365386 2.3:-18951869 MS.gene068723:intron
TTGCATATGGATTTGTTCAA+TGG 0.367870 2.3:-18951526 MS.gene068723:CDS
AAGGAGGAGAGAGAGCTTTA+GGG 0.372976 2.3:+18950300 None:intergenic
TTGGACAGAATCTAGTAGTA+TGG 0.375092 2.3:+18950162 None:intergenic
CTTGGTGAGCTTCAGTTAGC+TGG 0.379613 2.3:-18953105 MS.gene068723:CDS
TGGACTGGTTTAAATTTCAT+AGG 0.381642 2.3:-18951506 MS.gene068723:CDS
AATCCAAGGAGACATGAAAT+TGG 0.384730 2.3:+18949950 None:intergenic
TGGATATCAATGATAATGTT+AGG 0.394235 2.3:-18951455 MS.gene068723:CDS
ACACTCTGCGGACAAGGATT+TGG 0.397575 2.3:-18951836 MS.gene068723:CDS
TTGGCCTGCTCCCAATTCAT+TGG 0.400145 2.3:+18950354 None:intergenic
CTAAAGCTCTCTCTCCTCCT+TGG 0.416995 2.3:-18950298 MS.gene068723:CDS
ATGATATAGGGTTTATGGAT+TGG 0.419805 2.3:-18949275 MS.gene068723:intron
CCATGATCATCTGCTAATAG+AGG 0.432700 2.3:+18953282 None:intergenic
TGGACTAAACTGAATCTCTC+AGG 0.432935 2.3:-18949191 MS.gene068723:CDS
TGTCACTGGCGTAGCAGTTA+TGG 0.436994 2.3:-18953055 MS.gene068723:intron
ACAAGAGTTTCCAATGAATT+GGG 0.444602 2.3:-18950364 MS.gene068723:CDS
CAAGACCACAAATAGAGAAA+TGG 0.449315 2.3:-18953248 MS.gene068723:CDS
AATTCTTGGTTTAGTGTCAC+TGG 0.452462 2.3:-18953069 MS.gene068723:CDS
GAAATGGTGGAACAAAATCT+TGG 0.453708 2.3:-18953232 MS.gene068723:CDS
TTACCAATTTCATGTCTCCT+TGG 0.458402 2.3:-18949953 MS.gene068723:CDS
CAAAGAAATTTAAGAATACA+TGG 0.465514 2.3:-18951415 MS.gene068723:CDS
TTATGGATTGGTCTGATATG+TGG 0.475106 2.3:-18949263 MS.gene068723:CDS
GCAAAAGAATATCACATGTT+GGG 0.480088 2.3:-18951812 MS.gene068723:CDS
ATTGCTTACATGATCACTTA+CGG 0.481355 2.3:-18950667 MS.gene068723:CDS
CGCAAAAGAATATCACATGT+TGG 0.487458 2.3:-18951813 MS.gene068723:CDS
GCAGGGGTGGCTAGAGGATG+TGG 0.492719 2.3:-18950028 MS.gene068723:intron
GGTTATACTTTCAGCTATAC+CGG 0.496375 2.3:-18951567 MS.gene068723:CDS
TATGGATTTGTTCAATGGAC+TGG 0.501955 2.3:-18951521 MS.gene068723:CDS
CTAGAAACACTCTGCGGACA+AGG 0.502058 2.3:-18951842 MS.gene068723:CDS
TGCCTTCTGCTGCAATGGTA+TGG 0.502114 2.3:-18951337 MS.gene068723:intron
AAAGCTGAATCCATATATCA+TGG 0.508008 2.3:+18950245 None:intergenic
GTTGTTAAACAACAATAGGT+TGG 0.509921 2.3:+18949153 None:intergenic
ATGCTATATGCAAATAATCC+TGG 0.515013 2.3:+18951641 None:intergenic
GTTTCCAATGAATTGGGAGC+AGG 0.516102 2.3:-18950358 MS.gene068723:CDS
AGGATGTGGTTGGCAGCACT+TGG 0.517158 2.3:-18950014 MS.gene068723:CDS
GAAAGAGCAAAACATGCAAT+GGG 0.520409 2.3:-18950328 MS.gene068723:CDS
CCTCTATTAGCAGATGATCA+TGG 0.522596 2.3:-18953282 MS.gene068723:CDS
CTAGTCTTATGTTTGCAAGC+AGG 0.526210 2.3:+18951363 None:intergenic
AGAAAGAGCAAAACATGCAA+TGG 0.535451 2.3:-18950329 MS.gene068723:CDS
GGGTGGCTAGAGGATGTGGT+TGG 0.538894 2.3:-18950024 MS.gene068723:CDS
AGCGTTGCCTTCTGCTGCAA+TGG 0.539791 2.3:-18951342 MS.gene068723:CDS
GCATCTTGCAAAACTTGTTG+AGG 0.540279 2.3:-18951622 MS.gene068723:CDS
CAATGTCATGAGATTGATGA+AGG 0.548521 2.3:+18951699 None:intergenic
GATTGGTCTGATATGTGGAT+TGG 0.550595 2.3:-18949258 MS.gene068723:CDS
TACCATACCATTGCAGCAGA+AGG 0.554425 2.3:+18951335 None:intergenic
TATATGAAGTTTCTTATACC+AGG 0.554707 2.3:-18951659 MS.gene068723:CDS
ATGTTGGGAATTTATCTACA+AGG 0.560309 2.3:-18951797 MS.gene068723:CDS
GCAAATCGGTCTTAAATCCA+AGG 0.561263 2.3:+18949936 None:intergenic
ACTGGTTTAAATTTCATAGG+TGG 0.564576 2.3:-18951503 MS.gene068723:CDS
GGACTAAACTGAATCTCTCA+GGG 0.566867 2.3:-18949190 MS.gene068723:CDS
ATTCCAACATGAACTAATGC+CGG 0.579060 2.3:+18951548 None:intergenic
GACCACAAATAGAGAAATGG+TGG 0.585994 2.3:-18953245 MS.gene068723:CDS
GTGGATTGGTGTGTCAAACT+GGG 0.589183 2.3:-18949244 MS.gene068723:CDS
ACAATCTGTAGTAATGCCAC+TGG 0.591821 2.3:-18951588 MS.gene068723:CDS
ATACCGGCATTAGTTCATGT+TGG 0.592170 2.3:-18951551 MS.gene068723:CDS
ATACTACTAGATTCTGTCCA+AGG 0.600992 2.3:-18950160 MS.gene068723:CDS
AATAATGCAGGGGTGGCTAG+AGG 0.604681 2.3:-18950034 MS.gene068723:intron
AAATTTAAGAATACATGGAG+AGG 0.607514 2.3:-18951410 MS.gene068723:CDS
GCGGCAAGTATCATTTCAGA+TGG 0.611934 2.3:-18953315 MS.gene068723:CDS
GTAAGCAATGAATTCTGTGT+TGG 0.613351 2.3:+18950681 None:intergenic
TTAAACCTGATAAGACACCT+TGG 0.620465 2.3:+18950143 None:intergenic
ACGGTCTTAGTGCAGCTGCA+AGG 0.634465 2.3:-18950648 MS.gene068723:intron
AGTATGGAAATAGCAAGCAA+AGG 0.641984 2.3:+18950178 None:intergenic
TCAAAACAAAACATAAACCA+AGG 0.664534 2.3:+18950281 None:intergenic
AGTGAAATAGAAGTTGCAGT+CGG 0.711071 2.3:+18951479 None:intergenic
ATAGCTGAAAGTATAACCAG+TGG 0.720921 2.3:+18951572 None:intergenic
AAACAAAACATAAACCAAGG+AGG 0.722868 2.3:+18950284 None:intergenic
TGGATTGGTGTGTCAAACTG+GGG 0.738156 2.3:-18949243 MS.gene068723:CDS
GACTAAACTGAATCTCTCAG+GGG 0.747563 2.3:-18949189 MS.gene068723:CDS
GGTGCACTAGAAACACTCTG+CGG 0.812696 2.3:-18951848 MS.gene068723:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATCAATTAAAGTAAATT+TGG - chr2.3:18949928-18949947 MS.gene068723:CDS 10.0%
!!! TTATGTTATTTTGTTATTTA+TGG + chr2.3:18953055-18953074 None:intergenic 10.0%
!! AAAAAAATGATATATTTGCA+AGG - chr2.3:18951454-18951473 MS.gene068723:CDS 15.0%
!! AAAAACAATGATTAAACAAA+TGG + chr2.3:18953166-18953185 None:intergenic 15.0%
!! AATAAATTTGTAAGAATCAT+TGG + chr2.3:18949307-18949326 None:intergenic 15.0%
!! AATATTATACACATATTACA+TGG + chr2.3:18951337-18951356 None:intergenic 15.0%
!! GTAAGATTCATAATATATTA+AGG + chr2.3:18951575-18951594 None:intergenic 15.0%
!!! ACTAAAAGTTAATATGTTTT+TGG + chr2.3:18951512-18951531 None:intergenic 15.0%
!!! ATCATTTTTTTTCTTTCTTT+TGG - chr2.3:18951269-18951288 MS.gene068723:intron 15.0%
!!! ATTTTATTACTTGATTACTT+TGG - chr2.3:18949324-18949343 MS.gene068723:intron 15.0%
!!! TAGTTTTATAAATTACATGA+AGG - chr2.3:18949997-18950016 MS.gene068723:CDS 15.0%
!!! TATGTGTATAATATTCATTT+TGG - chr2.3:18951342-18951361 MS.gene068723:CDS 15.0%
!!! TATTTTATATTATGCAAGTT+TGG - chr2.3:18951601-18951620 MS.gene068723:CDS 15.0%
!!! TTTGAATGTTTTATGATATA+GGG - chr2.3:18953188-18953207 MS.gene068723:CDS 15.0%
!!! TTTTGAATGTTTTATGATAT+AGG - chr2.3:18953187-18953206 MS.gene068723:CDS 15.0%
!! AAAAAAAATGATATACTTGC+AGG + chr2.3:18951261-18951280 None:intergenic 20.0%
!! AAAAAAATGATATACTTGCA+GGG + chr2.3:18951260-18951279 None:intergenic 20.0%
!! AATATAGAAATTAGAAACGA+TGG + chr2.3:18950201-18950220 None:intergenic 20.0%
!! ATAACAAAATAACATAAGAC+AGG - chr2.3:18953058-18953077 MS.gene068723:CDS 20.0%
!! ATAGAAATTAGTACATTCAA+TGG - chr2.3:18953007-18953026 MS.gene068723:intron 20.0%
!! ATATCAACAATAAACATCTA+TGG + chr2.3:18949487-18949506 None:intergenic 20.0%
!! ATCATTAACAAAAACAACTT+AGG - chr2.3:18952398-18952417 MS.gene068723:intron 20.0%
!! CAAAGAAATTTAAGAATACA+TGG - chr2.3:18951060-18951079 MS.gene068723:intron 20.0%
!! CAATCATTATATCAATTGTT+TGG - chr2.3:18950727-18950746 MS.gene068723:intron 20.0%
!! CTGAAGAGTTTATATTAATA+AGG + chr2.3:18950405-18950424 None:intergenic 20.0%
!! CTTATTAATATAAACTCTTC+AGG - chr2.3:18950403-18950422 MS.gene068723:intron 20.0%
!! TAACAAAATAACATAAGACA+GGG - chr2.3:18953059-18953078 MS.gene068723:CDS 20.0%
!! TATGTTGAGATATAATAAAG+AGG + chr2.3:18952924-18952943 None:intergenic 20.0%
!! TCAAAACTATTTATCAAACA+AGG + chr2.3:18951223-18951242 None:intergenic 20.0%
!! TCATTAACAAAAACAACTTA+GGG - chr2.3:18952399-18952418 MS.gene068723:intron 20.0%
!! TTTATATGTATTCAAGTCAA+TGG - chr2.3:18949769-18949788 MS.gene068723:intron 20.0%
!!! ATTTTAAACAGAAATCTTGA+AGG + chr2.3:18951297-18951316 None:intergenic 20.0%
!!! CAACTAAATTTGAAATCTAA+GGG + chr2.3:18952768-18952787 None:intergenic 20.0%
!!! TCAACTAAATTTGAAATCTA+AGG + chr2.3:18952769-18952788 None:intergenic 20.0%
!!! TTTGTTTTGATACTTGTATT+TGG - chr2.3:18952207-18952226 MS.gene068723:intron 20.0%
! AAAGGGTTAAATAAAGTGAA+AGG + chr2.3:18951180-18951199 None:intergenic 25.0%
! AAAGTATATCCACTAACATA+AGG - chr2.3:18950317-18950336 MS.gene068723:CDS 25.0%
! AAATTTAAGAATACATGGAG+AGG - chr2.3:18951065-18951084 MS.gene068723:intron 25.0%
! AACAATGTGTTAAATAATGC+AGG - chr2.3:18952429-18952448 MS.gene068723:intron 25.0%
! ACAATGTGTTAAATAATGCA+GGG - chr2.3:18952430-18952449 MS.gene068723:intron 25.0%
! ACCAACGACTATATTAATAA+GGG - chr2.3:18952956-18952975 MS.gene068723:intron 25.0%
! ATTAACAAAAACAACTTAGG+GGG - chr2.3:18952401-18952420 MS.gene068723:intron 25.0%
! CAAAAGTTCTACATATTACA+AGG - chr2.3:18951398-18951417 MS.gene068723:CDS 25.0%
! CATTAACAAAAACAACTTAG+GGG - chr2.3:18952400-18952419 MS.gene068723:intron 25.0%
! CTAATTTCTATATTCAGAAC+TGG - chr2.3:18950207-18950226 MS.gene068723:CDS 25.0%
! GATATCAAGAATAATCATCT+TGG - chr2.3:18953132-18953151 MS.gene068723:CDS 25.0%
! TATATGAAGTTTCTTATACC+AGG - chr2.3:18950816-18950835 MS.gene068723:CDS 25.0%
! TATTATAACGTTATGAGTAG+TGG - chr2.3:18949821-18949840 MS.gene068723:intron 25.0%
! TCAAAACAAAACATAAACCA+AGG + chr2.3:18952197-18952216 None:intergenic 25.0%
! TCTACATATTACAAGGTTTA+GGG - chr2.3:18951405-18951424 MS.gene068723:CDS 25.0%
! TGATAGTCCTATTATCAAAA+AGG - chr2.3:18952257-18952276 MS.gene068723:intron 25.0%
! TGTAAGATCAATCATCAAAA+GGG + chr2.3:18951197-18951216 None:intergenic 25.0%
! TTAATCTTGTGACAACATTT+AGG + chr2.3:18950522-18950541 None:intergenic 25.0%
! TTCTACATATTACAAGGTTT+AGG - chr2.3:18951404-18951423 MS.gene068723:CDS 25.0%
! TTGTAAGATCAATCATCAAA+AGG + chr2.3:18951198-18951217 None:intergenic 25.0%
!! ATGTTAGTAATGTTAGTTTC+TGG - chr2.3:18950078-18950097 MS.gene068723:intron 25.0%
!! GTTTTATGATATAGGGTTTA+TGG - chr2.3:18953195-18953214 MS.gene068723:CDS 25.0%
!! TGGATATCAATGATAATGTT+AGG - chr2.3:18951020-18951039 MS.gene068723:intron 25.0%
!! TGTTAGTAATGTTAGTTTCT+GGG - chr2.3:18950079-18950098 MS.gene068723:intron 25.0%
!! TTAAATTCCTCATCTCTTTT+AGG - chr2.3:18952351-18952370 MS.gene068723:intron 25.0%
!! TTAGTGGATATACTTTAGTA+AGG + chr2.3:18950313-18950332 None:intergenic 25.0%
!! TTTTACCTTATACTGCAAAT+CGG + chr2.3:18952556-18952575 None:intergenic 25.0%
!!! TGAAAACATGAGTTGAATTT+TGG + chr2.3:18949281-18949300 None:intergenic 25.0%
!!! TGATTTTAATCACTCCAATA+TGG + chr2.3:18951380-18951399 None:intergenic 25.0%
!!! TGATTTTTTGAGAGAGTTTA+TGG - chr2.3:18950347-18950366 MS.gene068723:CDS 25.0%
AAACAAAACATAAACCAAGG+AGG + chr2.3:18952194-18952213 None:intergenic 30.0%
AAAGCTGAATCCATATATCA+TGG + chr2.3:18952233-18952252 None:intergenic 30.0%
AACTTAACCTAAAAGAGATG+AGG + chr2.3:18952361-18952380 None:intergenic 30.0%
AATGAATGTGTAATCGTGAT+AGG - chr2.3:18952715-18952734 MS.gene068723:intron 30.0%
AATGTGATTGTATATGACTG+CGG - chr2.3:18952026-18952045 MS.gene068723:intron 30.0%
ACAAGAGTTTCCAATGAATT+GGG - chr2.3:18952111-18952130 MS.gene068723:intron 30.0%
ACCCTTATTAATATAGTCGT+TGG + chr2.3:18952960-18952979 None:intergenic 30.0%
AGTGTTTCCGTTTAATAAGT+AGG + chr2.3:18952689-18952708 None:intergenic 30.0%
ATGAATGTGTAATCGTGATA+GGG - chr2.3:18952716-18952735 MS.gene068723:intron 30.0%
ATGATATAGGGTTTATGGAT+TGG - chr2.3:18953200-18953219 MS.gene068723:CDS 30.0%
ATGCTATATGCAAATAATCC+TGG + chr2.3:18950837-18950856 None:intergenic 30.0%
ATGTGACACTTAACATGAAA+CGG + chr2.3:18952587-18952606 None:intergenic 30.0%
ATGTTGGGAATTTATCTACA+AGG - chr2.3:18950678-18950697 MS.gene068723:CDS 30.0%
ATTGCTTACATGATCACTTA+CGG - chr2.3:18951808-18951827 MS.gene068723:CDS 30.0%
CAATACATGTACAAGTATGA+AGG + chr2.3:18952064-18952083 None:intergenic 30.0%
CAATGAGATAAAAAGTTGCA+AGG + chr2.3:18952500-18952519 None:intergenic 30.0%
CAATGTGTTAAATAATGCAG+GGG - chr2.3:18952431-18952450 MS.gene068723:intron 30.0%
CACCAACGACTATATTAATA+AGG - chr2.3:18952955-18952974 MS.gene068723:intron 30.0%
CTTATTAATATAGTCGTTGG+TGG + chr2.3:18952957-18952976 None:intergenic 30.0%
GCAAAAGAATATCACATGTT+GGG - chr2.3:18950663-18950682 MS.gene068723:CDS 30.0%
GTATACCCCTACTTATTAAA+CGG - chr2.3:18952679-18952698 MS.gene068723:intron 30.0%
GTGTTTCCGTTTAATAAGTA+GGG + chr2.3:18952688-18952707 None:intergenic 30.0%
TAGGGACTAAAAAAACAACA+AGG - chr2.3:18951423-18951442 MS.gene068723:CDS 30.0%
TGACAACATTTAGGTATGTT+TGG + chr2.3:18950513-18950532 None:intergenic 30.0%
TGTTTCCGTTTAATAAGTAG+GGG + chr2.3:18952687-18952706 None:intergenic 30.0%
TTGATTTATTCTGTCACAGT+TGG + chr2.3:18949916-18949935 None:intergenic 30.0%
TTGTATTTGGCCATGATATA+TGG - chr2.3:18952220-18952239 MS.gene068723:intron 30.0%
TTTAGTAAGGCAATTCAATC+AGG + chr2.3:18950300-18950319 None:intergenic 30.0%
! AATAAGTAGGGGTATACTAA+TGG + chr2.3:18952676-18952695 None:intergenic 30.0%
! ACTGGTTTAAATTTCATAGG+TGG - chr2.3:18950972-18950991 MS.gene068723:intron 30.0%
! ATTATGCAAGTTTGGAGTAT+TGG - chr2.3:18951609-18951628 MS.gene068723:CDS 30.0%
! CAACTTCATTGATTGCAATA+TGG - chr2.3:18951684-18951703 MS.gene068723:CDS 30.0%
! CATTGTTGCATAAATCCTTT+TGG - chr2.3:18951885-18951904 MS.gene068723:intron 30.0%
! CTATATTCAGAACTGGATTT+TGG - chr2.3:18950214-18950233 MS.gene068723:CDS 30.0%
! CTTGCAACTTTTTATCTCAT+TGG - chr2.3:18952498-18952517 MS.gene068723:intron 30.0%
! GTTAGTAATGTTAGTTTCTG+GGG - chr2.3:18950080-18950099 MS.gene068723:intron 30.0%
! TCATTGATTGCAATATGGTA+TGG - chr2.3:18951689-18951708 MS.gene068723:CDS 30.0%
! TTATGCAAGTTTGGAGTATT+GGG - chr2.3:18951610-18951629 MS.gene068723:CDS 30.0%
! TTGCATATGGATTTGTTCAA+TGG - chr2.3:18950949-18950968 MS.gene068723:intron 30.0%
!! GAAGTTGTTATTTGTGAATC+AGG + chr2.3:18951671-18951690 None:intergenic 30.0%
!! GCAAACTCCTTTTTGATAAT+AGG + chr2.3:18952267-18952286 None:intergenic 30.0%
!! TGGACTGGTTTAAATTTCAT+AGG - chr2.3:18950969-18950988 MS.gene068723:intron 30.0%
!!! GGTATTCTTTTTTGATGAAG+TGG + chr2.3:18952795-18952814 None:intergenic 30.0%
AATCCAAGGAGACATGAAAT+TGG + chr2.3:18952528-18952547 None:intergenic 35.0%
AATTCTTGGTTTAGTGTCAC+TGG - chr2.3:18949406-18949425 MS.gene068723:intron 35.0%
AGAAAGAGCAAAACATGCAA+TGG - chr2.3:18952146-18952165 MS.gene068723:intron 35.0%
AGTATGGAAATAGCAAGCAA+AGG + chr2.3:18952300-18952319 None:intergenic 35.0%
AGTGAAATAGAAGTTGCAGT+CGG + chr2.3:18950999-18951018 None:intergenic 35.0%
ATACTACTAGATTCTGTCCA+AGG - chr2.3:18952315-18952334 MS.gene068723:intron 35.0%
ATTCCAACATGAACTAATGC+CGG + chr2.3:18950930-18950949 None:intergenic 35.0%
CAAGACCACAAATAGAGAAA+TGG - chr2.3:18949227-18949246 MS.gene068723:CDS 35.0%
CAATGTCATGAGATTGATGA+AGG + chr2.3:18950779-18950798 None:intergenic 35.0%
CACAAGAGTTTCCAATGAAT+TGG - chr2.3:18952110-18952129 MS.gene068723:intron 35.0%
CGCAAAAGAATATCACATGT+TGG - chr2.3:18950662-18950681 MS.gene068723:CDS 35.0%
CTGCAACTTCTATTTCACTA+TGG - chr2.3:18951000-18951019 MS.gene068723:intron 35.0%
GAAAGAGCAAAACATGCAAT+GGG - chr2.3:18952147-18952166 MS.gene068723:intron 35.0%
GGTTATACTTTCAGCTATAC+CGG - chr2.3:18950908-18950927 MS.gene068723:intron 35.0%
GTAAGCAATGAATTCTGTGT+TGG + chr2.3:18951797-18951816 None:intergenic 35.0%
GTTCATGTTGGAATTGCATA+TGG - chr2.3:18950936-18950955 MS.gene068723:intron 35.0%
TCTTAGACTATAGGAAATCC+AGG + chr2.3:18951912-18951931 None:intergenic 35.0%
TGTACAAGTATGAAGGAACT+TGG + chr2.3:18952057-18952076 None:intergenic 35.0%
TTAAACCTGATAAGACACCT+TGG + chr2.3:18952335-18952354 None:intergenic 35.0%
TTACCAATTTCATGTCTCCT+TGG - chr2.3:18952522-18952541 MS.gene068723:intron 35.0%
TTCACTTTGATCTTAGCACA+TGG + chr2.3:18950249-18950268 None:intergenic 35.0%
TTCCACCATTTCTCTATTTG+TGG + chr2.3:18949235-18949254 None:intergenic 35.0%
TTGGACAGAATCTAGTAGTA+TGG + chr2.3:18952316-18952335 None:intergenic 35.0%
! AAGAGTATATGTTCCGAGTA+AGG + chr2.3:18950157-18950176 None:intergenic 35.0%
! ATAGCTGAAAGTATAACCAG+TGG + chr2.3:18950906-18950925 None:intergenic 35.0%
! ATTGCATGTTTTGCTCTTTC+TGG + chr2.3:18952147-18952166 None:intergenic 35.0%
! CAATTAAAATTTTGCCCGAG+TGG + chr2.3:18952845-18952864 None:intergenic 35.0%
! GAAATGGTGGAACAAAATCT+TGG - chr2.3:18949243-18949262 MS.gene068723:CDS 35.0%
! TATGGATTTGTTCAATGGAC+TGG - chr2.3:18950954-18950973 MS.gene068723:intron 35.0%
! TTTTGACAAGGCATGTCAAA+TGG - chr2.3:18953264-18953283 MS.gene068723:CDS 35.0%
!! CACTCGGGCAAAATTTTAAT+TGG - chr2.3:18952843-18952862 MS.gene068723:intron 35.0%
!! TAAGACCGATTTGCAGTATA+AGG - chr2.3:18952548-18952567 MS.gene068723:intron 35.0%
!! TTATGGATTGGTCTGATATG+TGG - chr2.3:18953212-18953231 MS.gene068723:CDS 35.0%
!!! GAAGTTTTGGTTTTCTTAGC+TGG - chr2.3:18951638-18951657 MS.gene068723:CDS 35.0%
!!! GTATTGGGCTTTTGAAGTTT+TGG - chr2.3:18951625-18951644 MS.gene068723:CDS 35.0%
ACAATCTGTAGTAATGCCAC+TGG - chr2.3:18950887-18950906 MS.gene068723:intron 40.0%
ACGATACAGTTGCGAAAAAC+AGG + chr2.3:18952873-18952892 None:intergenic 40.0%
ACTCCAATATGGTCTACATG+AGG + chr2.3:18951369-18951388 None:intergenic 40.0%
AGCGCGATTTCTTAGACTAT+AGG + chr2.3:18951921-18951940 None:intergenic 40.0%
ATACCGGCATTAGTTCATGT+TGG - chr2.3:18950924-18950943 MS.gene068723:intron 40.0%
ATAGGAAATCCAGGTCCAAA+AGG + chr2.3:18951903-18951922 None:intergenic 40.0%
CACACATGTCCTTATGTTAG+TGG + chr2.3:18950329-18950348 None:intergenic 40.0%
CCATGATCATCTGCTAATAG+AGG + chr2.3:18949196-18949215 None:intergenic 40.0%
CCTCTATTAGCAGATGATCA+TGG - chr2.3:18949193-18949212 MS.gene068723:CDS 40.0%
GACCACAAATAGAGAAATGG+TGG - chr2.3:18949230-18949249 MS.gene068723:CDS 40.0%
GACTAAACTGAATCTCTCAG+GGG - chr2.3:18953286-18953305 MS.gene068723:CDS 40.0%
GCAAATCGGTCTTAAATCCA+AGG + chr2.3:18952542-18952561 None:intergenic 40.0%
GGACTAAACTGAATCTCTCA+GGG - chr2.3:18953285-18953304 MS.gene068723:CDS 40.0%
TACAGTTGCGAAAAACAGGT+TGG + chr2.3:18952869-18952888 None:intergenic 40.0%
TATTCTTCTCTGTCTCAGGT+TGG - chr2.3:18950597-18950616 MS.gene068723:intron 40.0%
TGATAGGGTTCACGAATATG+TGG - chr2.3:18952731-18952750 MS.gene068723:intron 40.0%
TGGACTAAACTGAATCTCTC+AGG - chr2.3:18953284-18953303 MS.gene068723:CDS 40.0%
TGTGTTAAATAATGCAGGGG+TGG - chr2.3:18952434-18952453 MS.gene068723:intron 40.0%
TTATGAGTAGTGGCTCATAC+TGG - chr2.3:18949831-18949850 MS.gene068723:intron 40.0%
TTGGTTTCTGTCATGCTTGT+TGG - chr2.3:18949343-18949362 MS.gene068723:intron 40.0%
! GCTTTATTCTTCTCTGTCTC+AGG - chr2.3:18950593-18950612 MS.gene068723:intron 40.0%
! GTGCTACTCTTGCTAATTCT+TGG - chr2.3:18949392-18949411 MS.gene068723:intron 40.0%
! TGCATAAATCCTTTTGGACC+TGG - chr2.3:18951891-18951910 MS.gene068723:intron 40.0%
!! CATGTTTTGCTCTTTCTGGT+TGG + chr2.3:18952143-18952162 None:intergenic 40.0%
!! CTAGTCTTATGTTTGCAAGC+AGG + chr2.3:18951115-18951134 None:intergenic 40.0%
!! GATTGGTCTGATATGTGGAT+TGG - chr2.3:18953217-18953236 MS.gene068723:CDS 40.0%
!! GCATCTTGCAAAACTTGTTG+AGG - chr2.3:18950853-18950872 MS.gene068723:CDS 40.0%
!! GTGTGTTCATTGATTCGACA+CGG - chr2.3:18949518-18949537 MS.gene068723:intron 40.0%
AAGGAGGAGAGAGAGCTTTA+GGG + chr2.3:18952178-18952197 None:intergenic 45.0%
CTTTAACACACTCGCTTTCG+CGG - chr2.3:18951862-18951881 MS.gene068723:CDS 45.0%
GCAATTCAATCAGGTCTGTC+TGG + chr2.3:18950291-18950310 None:intergenic 45.0%
GCGGCAAGTATCATTTCAGA+TGG - chr2.3:18949160-18949179 MS.gene068723:CDS 45.0%
GGTCCTCATGTAGACCATAT+TGG - chr2.3:18951363-18951382 MS.gene068723:CDS 45.0%
GTGGCTCATACTGGAATAAG+AGG - chr2.3:18949840-18949859 MS.gene068723:intron 45.0%
GTTTCCAATGAATTGGGAGC+AGG - chr2.3:18952117-18952136 MS.gene068723:intron 45.0%
TACCATACCATTGCAGCAGA+AGG + chr2.3:18951143-18951162 None:intergenic 45.0%
TATGTTCCGAGTAAGGAGCT+AGG + chr2.3:18950150-18950169 None:intergenic 45.0%
TTGTCACTGATCTCGACAGT+TGG + chr2.3:18952008-18952027 None:intergenic 45.0%
! GTCATGCTTGTTGGTCATCT+TGG - chr2.3:18949352-18949371 MS.gene068723:intron 45.0%
! TAGTTTCTGGGGATAGAACC+CGG - chr2.3:18950091-18950110 MS.gene068723:intron 45.0%
! TCTGTCCAAGGTGTCTTATC+AGG - chr2.3:18952327-18952346 MS.gene068723:intron 45.0%
!! CTGTCTCAGGTTGGTTTAAG+TGG - chr2.3:18950606-18950625 MS.gene068723:intron 45.0%
!! GGGCACTCTTACTTTTGACA+AGG - chr2.3:18953252-18953271 MS.gene068723:CDS 45.0%
!! GTGGATTGGTGTGTCAAACT+GGG - chr2.3:18953231-18953250 MS.gene068723:CDS 45.0%
!! TGGATTGGTGTGTCAAACTG+GGG - chr2.3:18953232-18953251 MS.gene068723:CDS 45.0%
!! TGTGGATTGGTGTGTCAAAC+TGG - chr2.3:18953230-18953249 MS.gene068723:CDS 45.0%
!! ATATATAAATTAATCAATAA+GGG + chr2.3:18949662-18949681 None:intergenic 5.0%
!! TATATATAAATTAATCAATA+AGG + chr2.3:18949663-18949682 None:intergenic 5.0%
!!! TTTTAAAATTTTATCTAAAT+TGG + chr2.3:18949961-18949980 None:intergenic 5.0%
AATAATGCAGGGGTGGCTAG+AGG - chr2.3:18952441-18952460 MS.gene068723:intron 50.0%
ACACTCTGCGGACAAGGATT+TGG - chr2.3:18950639-18950658 MS.gene068723:intron 50.0%
AGGGTTCACGAATATGTGGC+AGG - chr2.3:18952735-18952754 MS.gene068723:intron 50.0%
AGTCACGTGGGTGTTTGTGT+TGG + chr2.3:18952821-18952840 None:intergenic 50.0%
AGTGCAGCTGCAAGGTTAGT+TGG - chr2.3:18951835-18951854 MS.gene068723:CDS 50.0%
CAAGGAGGAGAGAGAGCTTT+AGG + chr2.3:18952179-18952198 None:intergenic 50.0%
CACCAACCTAGCTCCTTACT+CGG - chr2.3:18950141-18950160 MS.gene068723:intron 50.0%
CTAAAGCTCTCTCTCCTCCT+TGG - chr2.3:18952177-18952196 MS.gene068723:intron 50.0%
CTAAGGGACGTTGAAGGATG+AGG + chr2.3:18950119-18950138 None:intergenic 50.0%
CTAGAAACACTCTGCGGACA+AGG - chr2.3:18950633-18950652 MS.gene068723:intron 50.0%
CTTGGTGAGCTTCAGTTAGC+TGG - chr2.3:18949370-18949389 MS.gene068723:intron 50.0%
GGTGCACTAGAAACACTCTG+CGG - chr2.3:18950627-18950646 MS.gene068723:intron 50.0%
TGCCTTCTGCTGCAATGGTA+TGG - chr2.3:18951138-18951157 MS.gene068723:intron 50.0%
TGTCACTGGCGTAGCAGTTA+TGG - chr2.3:18949420-18949439 MS.gene068723:intron 50.0%
TTCCGAGTAAGGAGCTAGGT+TGG + chr2.3:18950146-18950165 None:intergenic 50.0%
TTGGCCTGCTCCCAATTCAT+TGG + chr2.3:18952124-18952143 None:intergenic 50.0%
! AGTTTCTGGGGATAGAACCC+GGG - chr2.3:18950092-18950111 MS.gene068723:intron 50.0%
! GTCACAGTTGGTGCTTTCAG+AGG + chr2.3:18949904-18949923 None:intergenic 50.0%
!! GAGCTAGGTTGGTGAGCTAA+GGG + chr2.3:18950135-18950154 None:intergenic 50.0%
ACACCCACGTGACTTCCACT+CGG - chr2.3:18952827-18952846 MS.gene068723:intron 55.0%
ACGGTCTTAGTGCAGCTGCA+AGG - chr2.3:18951827-18951846 MS.gene068723:CDS 55.0%
ACGTTGAAGGATGAGGTCCC+GGG + chr2.3:18950112-18950131 None:intergenic 55.0%
AGCGTTGCCTTCTGCTGCAA+TGG - chr2.3:18951133-18951152 MS.gene068723:intron 55.0%
AGGATGTGGTTGGCAGCACT+TGG - chr2.3:18952461-18952480 MS.gene068723:intron 55.0%
GACGTTGAAGGATGAGGTCC+CGG + chr2.3:18950113-18950132 None:intergenic 55.0%
GGTGAGCTAAGGGACGTTGA+AGG + chr2.3:18950125-18950144 None:intergenic 55.0%
! CGTGGGTGTTTGTGTTGGTC+TGG + chr2.3:18952816-18952835 None:intergenic 55.0%
!! GGAGCTAGGTTGGTGAGCTA+AGG + chr2.3:18950136-18950155 None:intergenic 55.0%
CACCCACGTGACTTCCACTC+GGG - chr2.3:18952828-18952847 MS.gene068723:intron 60.0%
GGGTGGCTAGAGGATGTGGT+TGG - chr2.3:18952451-18952470 MS.gene068723:intron 60.0%
TGCCCGAGTGGAAGTCACGT+GGG + chr2.3:18952833-18952852 None:intergenic 60.0%
TTGCCCGAGTGGAAGTCACG+TGG + chr2.3:18952834-18952853 None:intergenic 60.0%
GCAGGGGTGGCTAGAGGATG+TGG - chr2.3:18952447-18952466 MS.gene068723:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 18949157 18953340 18949157 ID=MS.gene068723
chr2.3 mRNA 18949157 18953340 18949157 ID=MS.gene068723.t1;Parent=MS.gene068723
chr2.3 exon 18953056 18953340 18953056 ID=MS.gene068723.t1.exon1;Parent=MS.gene068723.t1
chr2.3 CDS 18953056 18953340 18953056 ID=cds.MS.gene068723.t1;Parent=MS.gene068723.t1
chr2.3 exon 18951338 18951882 18951338 ID=MS.gene068723.t1.exon2;Parent=MS.gene068723.t1
chr2.3 CDS 18951338 18951882 18951338 ID=cds.MS.gene068723.t1;Parent=MS.gene068723.t1
chr2.3 exon 18950792 18950878 18950792 ID=MS.gene068723.t1.exon3;Parent=MS.gene068723.t1
chr2.3 CDS 18950792 18950878 18950792 ID=cds.MS.gene068723.t1;Parent=MS.gene068723.t1
chr2.3 exon 18950649 18950705 18950649 ID=MS.gene068723.t1.exon4;Parent=MS.gene068723.t1
chr2.3 CDS 18950649 18950705 18950649 ID=cds.MS.gene068723.t1;Parent=MS.gene068723.t1
chr2.3 exon 18950149 18950387 18950149 ID=MS.gene068723.t1.exon5;Parent=MS.gene068723.t1
chr2.3 CDS 18950149 18950387 18950149 ID=cds.MS.gene068723.t1;Parent=MS.gene068723.t1
chr2.3 exon 18949928 18950046 18949928 ID=MS.gene068723.t1.exon6;Parent=MS.gene068723.t1
chr2.3 CDS 18949928 18950046 18949928 ID=cds.MS.gene068723.t1;Parent=MS.gene068723.t1
chr2.3 exon 18949157 18949288 18949157 ID=MS.gene068723.t1.exon7;Parent=MS.gene068723.t1
chr2.3 CDS 18949157 18949288 18949157 ID=cds.MS.gene068723.t1;Parent=MS.gene068723.t1
Gene Sequence

>MS.gene068723

ATGGCGGCAAGTATCATTTCAGATGGCACAGAAGCACCTCTATTAGCAGATGATCATGGAAAACAAAACACAAGACCACAAATAGAGAAATGGTGGAACAAAATCTTGGACATAGAAGAAGCCAAAATTCAACTCATGTTTTCATTACCAATGATTCTTACAAATTTATTTTATTACTTGATTACTTTGGTTTCTGTCATGCTTGTTGGTCATCTTGGTGAGCTTCAGTTAGCTGGTGCTACTCTTGCTAATTCTTGGTTTAGTGTCACTGGCGTAGCAGTTATGGTAATTAATTTACTATTTTTCATTATTATAGTTTGTTCATTTCCATAGATGTTTATTGTTGATATGAACAATTCTTGTGTGTTCATTGATTCGACACGGTCAATCAAAAACATTAAGTACAATGTTATTTAATAATAACTTCAACATGATATGTGATGTCTCTTTCTTTATTATTTTAAAAAATGTAGAACTCTTGATCAAAGTAAAAATTCTTGAACCCTTATTGATTAATTTATATATACATTCTTATGTATGAATAACTAGTCAAATGTAAATTTATATGTATGTTTGTATATATGACAGAAAATCAGAAATTTCTTTTGTTTATTTATATGTATTCAAGTCAATGGTTTGTTTAGAAATCTGATTGATGCTAGAATATTATAACGTTATGAGTAGTGGCTCATACTGGAATAAGAGGTTTGACTTTATATCTTTTGATTGCTTGCAACAACTTCTCCTCTGAAAGCACCAACTGTGACAGAATAAATCAATTAAAGTAAATTTGGAAAAGCTCCAATTTAGATAAAATTTTAAAATGTACTTTTTTGTTCATAGTTTTATAAATTACATGAAGGAAAAGAAATATTAAAAAGCTCCAATTTAGATAAAATTTTAAAAAGTACTTTTTTGTTTATGTTAGTAATGTTAGTTTCTGGGGATAGAACCCGGGACCTCATCCTTCAACGTCCCTTAGCTCACCAACCTAGCTCCTTACTCGGAACATATACTCTTAACATAATGCTAAAATTTCAACCATCGTTTCTAATTTCTATATTCAGAACTGGATTTTGGCTTCATTTACCATGTGCTAAGATCAAAGTGAATGCAAGTTTAACATCATAACCAGACAGACCTGATTGAATTGCCTTACTAAAGTATATCCACTAACATAAGGACATGTGTGATTTTTTGAGAGAGTTTATGGAAAGCTTATCAAATGTTCATAAGTTTATTTCACCTTATTAATATAAACTCTTCAGGATAGCTCATGAACACAGTTTAACTTTGCTTTATCTTTTGTTATAGAAATAGCTTATATATAAACACTTGTATGATCTTGTTTATCCAAACATACCTAAATGTTGTCACAAGATTAAACTATATGCTCTTGTTTCTCAAAAAATAAATAATGAAACTCTATGCTCTTTGCTTTATTCTTCTCTGTCTCAGGTTGGTTTAAGTGGTGCACTAGAAACACTCTGCGGACAAGGATTTGGCGCAAAAGAATATCACATGTTGGGAATTTATCTACAAGGCTCTTGCATTATATCTTTCATTTTTTCAATCATTATATCAATTGTTTGGTTCTACACAGAACACATTCTAGTGTTCCTTCATCAATCTCATGACATTGCTAGAACAGCAGCACTCTATATGAAGTTTCTTATACCAGGATTATTTGCATATAGCATCTTGCAAAACTTGTTGAGGTTTCTACAAACACAATCTGTAGTAATGCCACTGGTTATACTTTCAGCTATACCGGCATTAGTTCATGTTGGAATTGCATATGGATTTGTTCAATGGACTGGTTTAAATTTCATAGGTGGACCGACTGCAACTTCTATTTCACTATGGATATCAATGATAATGTTAGGATTATATGTAATGTATGCAAAGAAATTTAAGAATACATGGAGAGGATTCTCAATGCAATCATTTGATTACCTGCTTGCAAACATAAGACTAGCGTTGCCTTCTGCTGCAATGGTATGGTATGAGTTTCTAATTGCCTTTCACTTTATTTAACCCTTTTGATGATTGATCTTACAAAACCTTGTTTGATAAATAGTTTTGAATATGTTCTTTTGTCCCTGCAAGTATATCATTTTTTTTCTTTCTTTTGGAGCCTTCAAGATTTCTGTTTAAAATTTGTTTATGTTTGAATTCCATGTAATATGTGTATAATATTCATTTTGGTCCTCATGTAGACCATATTGGAGTGATTAAAATCAAAAGTTCTACATATTACAAGGTTTAGGGACTAAAAAAACAACAAGGATGAAAATAAAAAAATGATATATTTGCAAGGACTGAAATAAAAAAGTTTTATATTTGCATAGACCAAAAACATATTAACTTTTAGTTTGAACACTATCAGACTTTTGTTGCTGTTTAATGTAAGATCCTTAATATATTATGAATCTTACTATTTTTATTTTATATTATGCAAGTTTGGAGTATTGGGCTTTTGAAGTTTTGGTTTTCTTAGCTGGATTAATGCCTGATTCACAAATAACAACTTCATTGATTGCAATATGGTATGGTTCTCATATCTTTATATTTCTGTAACTTAAATGAAGAGAAGAAAGATTCACTATTGTTTTTCTTCAAAACAATCATGCAGTGCCAACACAGAATTCATTGCTTACATGATCACTTACGGTCTTAGTGCAGCTGCAAGGTTAGTTGGTCTACTTTAACACACTCGCTTTCGCGGCATTGTTGCATAAATCCTTTTGGACCTGGATTTCCTATAGTCTAAGAAATCGCGCTGTCAAGATATCTCAATCGTTAGATAAAGATCAATGACTCATATGATGAGACATCAAATTTTTATCCAACTGTCGAGATCAGTGACAATGTGATTGTATATGACTGCGGACAATCCAAGTTCCTTCATACTTGTACATGTATTGAAAGATTGAAACTTGAACTTTTATAGCACAAGAGTTTCCAATGAATTGGGAGCAGGCCAACCAGAAAGAGCAAAACATGCAATGGGAGTCACCCTAAAGCTCTCTCTCCTCCTTGGTTTATGTTTTGTTTTGATACTTGTATTTGGCCATGATATATGGATTCAGCTTTTTAGTGATAGTCCTATTATCAAAAAGGAGTTTGCTTCAGTGACACCTTTGCTTGCTATTTCCATACTACTAGATTCTGTCCAAGGTGTCTTATCAGGTTTAAATTCCTCATCTCTTTTAGGTTAAGTTTTCTCTACAACATGATTATCATTAACAAAAACAACTTAGGGGGTAAGTAACAATGTGTTAAATAATGCAGGGGTGGCTAGAGGATGTGGTTGGCAGCACTTGGCTGTTTATGTCAACCTTGCAACTTTTTATCTCATTGGCTTACCAATTTCATGTCTCCTTGGATTTAAGACCGATTTGCAGTATAAGGTAAAATTCTCTTTCCGTTTCATGTTAAGTGTCACATTTGTAATATTTGTTTGTCTCATTATTTATCATTTTAGAAAATCAATAAAGTATTAATTTTTTTTTTCCATTAGTATACCCCTACTTATTAAACGGAAACACTAGATTAAATGAATGTGTAATCGTGATAGGGTTCACGAATATGTGGCAGGTTAGTTTCCCTTAGATTTCAAATTTAGTTGACTACCACTTCATCAAAAAAGAATACCAGACCAACACAAACACCCACGTGACTTCCACTCGGGCAAAATTTTAATTGGCCAACCTGTTTTTCGCAACTGTATCGTGATAAATATCTTATAGATATATAGCATTCCTCTTTATTATATCTCAACATACAAAACTACTCCACCAACGACTATATTAATAAGGGTACTTTAGTAAATAAATCTTATTTTATCATAGAAATTAGTACATTCAATGGTTTTCTTAAGCAATGTGCATAACCATAAATAACAAAATAACATAAGACAGGGACTAATGTAGTTATTATTTCACAAAATCACAGTGTAATTATGTTTGTGTTGATATCAAGAATAATCATCTTGGTTTTGCATCCATTTGTTTAATCATTGTTTTTCTTTTGAATGTTTTATGATATAGGGTTTATGGATTGGTCTGATATGTGGATTGGTGTGTCAAACTGGGGCACTCTTACTTTTGACAAGGCATGTCAAATGGACTAAACTGAATCTCTCAGGGGACAAAGATAAAGACCAACCTATTGTTGTTTAA
Protein sequence

>MS.gene068723.t1

MAASIISDGTEAPLLADDHGKQNTRPQIEKWWNKILDIEEAKIQLMFSLPMILTNLFYYLITLVSVMLVGHLGELQLAGATLANSWFSVTGVAVMVGLSGALETLCGQGFGAKEYHMLGIYLQGSCIISFIFSIIISIVWFYTEHILVFLHQSHDIARTAALYMKFLIPGLFAYSILQNLLRFLQTQSVVMPLVILSAIPALVHVGIAYGFVQWTGLNFIGGPTATSISLWISMIMLGLYVMYAKKFKNTWRGFSMQSFDYLLANIRLALPSAAMVCLEYWAFEVLVFLAGLMPDSQITTSLIAICANTEFIAYMITYGLSAAASTRVSNELGAGQPERAKHAMGVTLKLSLLLGLCFVLILVFGHDIWIQLFSDSPIIKKEFASVTPLLAISILLDSVQGVLSGVARGCGWQHLAVYVNLATFYLIGLPISCLLGFKTDLQYKGLWIGLICGLVCQTGALLLLTRHVKWTKLNLSGDKDKDQPIVV