Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068792.t1 | XP_013464732.1 | 97.6 | 253 | 6 | 0 | 1 | 253 | 1 | 253 | 1.60E-144 | 521.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068792.t1 | P24805 | 40.9 | 149 | 87 | 1 | 1 | 149 | 1 | 148 | 3.1e-27 | 123.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068792.t1 | B7FHV5 | 97.6 | 253 | 6 | 0 | 1 | 253 | 1 | 253 | 1.2e-144 | 521.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052166 | MS.gene068792 | 0.811422 | 7.06E-51 | -1.69E-46 |
| MS.gene056623 | MS.gene068792 | 0.850572 | 1.56E-60 | -1.69E-46 |
| MS.gene059856 | MS.gene068792 | 0.950889 | 6.45E-109 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068792.t1 | MTR_2g079670 | 97.628 | 253 | 6 | 0 | 1 | 253 | 1 | 253 | 0.0 | 516 |
| MS.gene068792.t1 | MTR_2g079670 | 96.859 | 191 | 6 | 0 | 1 | 191 | 1 | 191 | 2.90e-137 | 384 |
| MS.gene068792.t1 | MTR_2g079670 | 98.361 | 183 | 3 | 0 | 71 | 253 | 22 | 204 | 5.19e-135 | 379 |
| MS.gene068792.t1 | MTR_2g013140 | 66.000 | 250 | 84 | 1 | 1 | 250 | 1 | 249 | 2.50e-117 | 336 |
| MS.gene068792.t1 | MTR_4g088955 | 60.956 | 251 | 97 | 1 | 1 | 250 | 64 | 314 | 7.38e-107 | 311 |
| MS.gene068792.t1 | MTR_2g013140 | 64.398 | 191 | 67 | 1 | 1 | 191 | 1 | 190 | 2.13e-81 | 242 |
| MS.gene068792.t1 | MTR_1g041380 | 47.012 | 251 | 124 | 4 | 1 | 247 | 1 | 246 | 4.30e-77 | 234 |
| MS.gene068792.t1 | MTR_1g102680 | 47.222 | 252 | 122 | 5 | 1 | 247 | 1 | 246 | 1.41e-76 | 232 |
| MS.gene068792.t1 | MTR_2g022520 | 41.631 | 233 | 127 | 6 | 1 | 230 | 1 | 227 | 4.39e-55 | 177 |
| MS.gene068792.t1 | MTR_4g117050 | 39.056 | 233 | 134 | 6 | 1 | 230 | 1 | 228 | 7.88e-54 | 174 |
| MS.gene068792.t1 | MTR_1g041380 | 44.560 | 193 | 100 | 3 | 1 | 191 | 1 | 188 | 1.27e-51 | 167 |
| MS.gene068792.t1 | MTR_4g117050 | 44.242 | 165 | 89 | 2 | 67 | 230 | 21 | 183 | 3.60e-47 | 155 |
| MS.gene068792.t1 | MTR_1g019625 | 75.926 | 54 | 6 | 1 | 113 | 159 | 14 | 67 | 3.26e-19 | 79.3 |
| MS.gene068792.t1 | MTR_7g059340 | 76.316 | 38 | 9 | 0 | 114 | 151 | 18 | 55 | 1.06e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068792.t1 | AT4G27450 | 78.629 | 248 | 52 | 1 | 1 | 247 | 1 | 248 | 3.65e-147 | 411 |
| MS.gene068792.t1 | AT3G15450 | 68.675 | 249 | 74 | 3 | 1 | 247 | 1 | 247 | 3.06e-125 | 356 |
| MS.gene068792.t1 | AT3G15450 | 66.839 | 193 | 60 | 3 | 1 | 191 | 1 | 191 | 4.51e-89 | 263 |
| MS.gene068792.t1 | AT5G43830 | 48.810 | 252 | 119 | 4 | 1 | 247 | 1 | 247 | 5.78e-80 | 241 |
| MS.gene068792.t1 | AT3G15450 | 64.286 | 182 | 58 | 4 | 1 | 180 | 1 | 177 | 2.40e-75 | 227 |
| MS.gene068792.t1 | AT3G22850 | 44.939 | 247 | 133 | 1 | 1 | 247 | 1 | 244 | 6.30e-74 | 226 |
| MS.gene068792.t1 | AT5G19140 | 42.241 | 232 | 127 | 5 | 1 | 230 | 1 | 227 | 2.92e-56 | 180 |
| MS.gene068792.t1 | AT5G19140 | 48.521 | 169 | 84 | 2 | 63 | 230 | 25 | 191 | 4.18e-51 | 166 |
| MS.gene068792.t1 | AT5G19140 | 39.655 | 232 | 121 | 6 | 1 | 230 | 1 | 215 | 2.42e-48 | 159 |
Find 0 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTTTTAACTCTAAATA+CGG | - | 15122:10162-10181 | MS.gene068792:intergenic | 10.0% |
| !!! | ATCAATTTTTCCAATAAAAA+AGG | - | 15122:9346-9365 | MS.gene068792:intergenic | 15.0% |
| !!! | ATTTTGTTTTCAAGATAAAT+GGG | - | 15122:9978-9997 | MS.gene068792:intergenic | 15.0% |
| !! | AAAACAAAATCTCTTCAATT+TGG | + | 15122:9988-10007 | MS.gene068792:intron | 20.0% |
| !! | TCCAACATAAAAGTTTAATA+CGG | + | 15122:9257-9276 | MS.gene068792:intron | 20.0% |
| !! | TTTGTTAGATCAATATGATA+CGG | + | 15122:10121-10140 | MS.gene068792:intron | 20.0% |
| !!! | CATCTTTAATTCCTTTTATA+AGG | + | 15122:9224-9243 | MS.gene068792:intron | 20.0% |
| !!! | GATTTTGTTTTCAAGATAAA+TGG | - | 15122:9979-9998 | MS.gene068792:intergenic | 20.0% |
| !!! | TTTTAGTACACCTTTTTTTA+TGG | + | 15122:9098-9117 | MS.gene068792:intron | 20.0% |
| ! | AATACTTGTTCTATGAACTT+TGG | + | 15122:8982-9001 | MS.gene068792:CDS | 25.0% |
| ! | AGAATTGTTAACAAAGAACA+TGG | - | 15122:9762-9781 | MS.gene068792:intergenic | 25.0% |
| ! | TTCATAGAACAAGTATTATC+AGG | - | 15122:8979-8998 | MS.gene068792:intergenic | 25.0% |
| !! | ACCGTATTAAACTTTTATGT+TGG | - | 15122:9261-9280 | MS.gene068792:intergenic | 25.0% |
| !! | TCCTTTTATAAGGATGAATT+CGG | + | 15122:9234-9253 | MS.gene068792:intron | 25.0% |
| !!! | ATGATGTTTGTTTTTTATGC+AGG | + | 15122:9805-9824 | MS.gene068792:intron | 25.0% |
| !!! | TGATGTTTGTTTTTTATGCA+GGG | + | 15122:9806-9825 | MS.gene068792:intron | 25.0% |
| !!! | TTTTGAAATCATTGAATGCA+GGG | + | 15122:10313-10332 | MS.gene068792:intron | 25.0% |
| !!! | TTTTTGAAATCATTGAATGC+AGG | + | 15122:10312-10331 | MS.gene068792:intron | 25.0% |
| AACCTTTGATGAACAGAAAA+AGG | - | 15122:9042-9061 | MS.gene068791:intergenic | 30.0% | |
| ACATATACTGCTTATTCTTG+GGG | + | 15122:9511-9530 | MS.gene068792:CDS | 30.0% | |
| ACCGAATTCATCCTTATAAA+AGG | - | 15122:9238-9257 | MS.gene068792:intergenic | 30.0% | |
| AGAATTTCTTCAGAAAGCTT+AGG | - | 15122:8934-8953 | MS.gene068792:intergenic | 30.0% | |
| ATAAGCTTCAATCAGAAACA+TGG | - | 15122:9598-9617 | MS.gene068792:intergenic | 30.0% | |
| ATGTTATTAGTGCTAAGAGA+TGG | + | 15122:9457-9476 | MS.gene068792:intron | 30.0% | |
| ATTACATCATAACCAACATG+TGG | + | 15122:10015-10034 | MS.gene068792:intron | 30.0% | |
| ATTGATGTTACTGACCAAAT+TGG | + | 15122:9360-9379 | MS.gene068792:intron | 30.0% | |
| CAAAGACGTGTATTATGTTA+CGG | + | 15122:9301-9320 | MS.gene068792:intron | 30.0% | |
| CACTTCAAACAAATTGTCAA+AGG | + | 15122:10278-10297 | MS.gene068792:intron | 30.0% | |
| GAACTTATCAACCTTAAAGT+TGG | - | 15122:10439-10458 | MS.gene068792:intergenic | 30.0% | |
| GACATATACTGCTTATTCTT+GGG | + | 15122:9510-9529 | MS.gene068792:CDS | 30.0% | |
| TAGATCAATATGATACGGTT+TGG | + | 15122:10126-10145 | MS.gene068792:intron | 30.0% | |
| TGACATATACTGCTTATTCT+TGG | + | 15122:9509-9528 | MS.gene068792:CDS | 30.0% | |
| TTCGTAGCAACCATAAAAAA+AGG | - | 15122:9111-9130 | MS.gene068792:intergenic | 30.0% | |
| ! | AAGCAATATGGTTTGTCAAA+GGG | + | 15122:9561-9580 | MS.gene068792:CDS | 30.0% |
| ! | TGTTTCTGATTGAAGCTTAT+AGG | + | 15122:9598-9617 | MS.gene068792:CDS | 30.0% |
| ! | TTTTGTAGCAACCATGTTAT+TGG | - | 15122:9418-9437 | MS.gene068792:intergenic | 30.0% |
| !! | ATTTTTGCAGGTTGTTTTGT+GGG | + | 15122:9481-9500 | MS.gene068792:intron | 30.0% |
| !! | TATTTTTGCAGGTTGTTTTG+TGG | + | 15122:9480-9499 | MS.gene068792:intron | 30.0% |
| !!! | CAGTTGCATACCTTTTTTAT+TGG | + | 15122:9333-9352 | MS.gene068792:intron | 30.0% |
| AACCCTCTTGTACAACATTA+AGG | - | 15122:9898-9917 | MS.gene068791:intergenic | 35.0% | |
| AAGTGTCTGAGTCTCTAATT+TGG | - | 15122:10263-10282 | MS.gene068791:intergenic | 35.0% | |
| AATGATTGTTGGATCGAGAT+CGG | - | 15122:10203-10222 | MS.gene068792:intergenic | 35.0% | |
| ACCATATTGCTTGTTCAGTA+AGG | - | 15122:9553-9572 | MS.gene068792:intergenic | 35.0% | |
| AGTTCCTTAACAACTTGATC+TGG | - | 15122:9651-9670 | MS.gene068792:intergenic | 35.0% | |
| CAAAGAATCGCAATCATATG+TGG | + | 15122:9176-9195 | MS.gene068792:intron | 35.0% | |
| CAAGTTGTTAAGGAACTTGA+TGG | + | 15122:9654-9673 | MS.gene068792:CDS | 35.0% | |
| CGTAACATAATACACGTCTT+TGG | - | 15122:9303-9322 | MS.gene068792:intergenic | 35.0% | |
| GACCTTAATGTTGTACAAGA+GGG | + | 15122:9893-9912 | MS.gene068792:CDS | 35.0% | |
| GCTAACACATCAATGATTGT+TGG | - | 15122:10214-10233 | MS.gene068792:intergenic | 35.0% | |
| GTTGTTTATGACAGCACATA+TGG | + | 15122:9690-9709 | MS.gene068792:CDS | 35.0% | |
| TCCTTACTGAACAAGCAATA+TGG | + | 15122:9549-9568 | MS.gene068792:CDS | 35.0% | |
| TGAACAGAAAAAGGCTTATC+AGG | - | 15122:9033-9052 | MS.gene068792:intergenic | 35.0% | |
| TGAATCCAATTGGATGGAAT+GGG | + | 15122:10491-10510 | MS.gene068792:CDS | 35.0% | |
| TGACCTTAATGTTGTACAAG+AGG | + | 15122:9892-9911 | MS.gene068792:CDS | 35.0% | |
| TGTATGTTTCATAGTGAAGG+AGG | + | 15122:10336-10355 | MS.gene068792:CDS | 35.0% | |
| TGTGCTGTCATAAACAACAA+AGG | - | 15122:9688-9707 | MS.gene068792:intergenic | 35.0% | |
| TTAAGCCTTTGTATGATGCA+GGG | - | 15122:8906-8925 | MS.gene068792:intergenic | 35.0% | |
| TTTAAGCCTTTGTATGATGC+AGG | - | 15122:8907-8926 | MS.gene068792:intergenic | 35.0% | |
| TTTAATACGGTATCAGAGTC+TGG | + | 15122:9270-9289 | MS.gene068792:intron | 35.0% | |
| ! | AGCCTTTTTCTGTTCATCAA+AGG | + | 15122:9037-9056 | MS.gene068792:CDS | 35.0% |
| ! | ATGTTGGCAATTTTCCACAA+AGG | + | 15122:8850-8869 | MS.gene068792:CDS | 35.0% |
| ! | ATTGCCTTCAACTTGTTCAA+TGG | - | 15122:10381-10400 | MS.gene068792:intergenic | 35.0% |
| ! | CAAGCAATATGGTTTGTCAA+AGG | + | 15122:9560-9579 | MS.gene068792:CDS | 35.0% |
| !! | ATGGTGGATTGAAGTTGTAT+TGG | + | 15122:9834-9853 | MS.gene068792:CDS | 35.0% |
| !! | CTAAGAGATGGTATTTTTGC+AGG | + | 15122:9469-9488 | MS.gene068792:intron | 35.0% |
| !! | TGGTGGATTGAAGTTGTATT+GGG | + | 15122:9835-9854 | MS.gene068792:CDS | 35.0% |
| !!! | TTTTTTATGCAGGGTTCTGA+TGG | + | 15122:9815-9834 | MS.gene068792:intron | 35.0% |
| AATAGCCCTGCATCATACAA+AGG | + | 15122:8898-8917 | MS.gene068792:CDS | 40.0% | |
| ATTTGCTTGCGCCAATAACA+TGG | + | 15122:9404-9423 | MS.gene068792:intron | 40.0% | |
| CAATTGATACCTGCTGGAAA+AGG | - | 15122:9938-9957 | MS.gene068792:intergenic | 40.0% | |
| CATCATAACCAACATGTGGT+TGG | + | 15122:10019-10038 | MS.gene068792:intron | 40.0% | |
| GATGTTACTGACCAAATTGG+CGG | + | 15122:9363-9382 | MS.gene068792:intron | 40.0% | |
| GCTAAACAATTGATACCTGC+TGG | - | 15122:9944-9963 | MS.gene068792:intergenic | 40.0% | |
| GGGTGTATGTTTCATAGTGA+AGG | + | 15122:10333-10352 | MS.gene068792:intron | 40.0% | |
| GTGAATCCAATTGGATGGAA+TGG | + | 15122:10490-10509 | MS.gene068792:CDS | 40.0% | |
| TCCTTAACAACTTGATCTGG+TGG | - | 15122:9648-9667 | MS.gene068792:intergenic | 40.0% | |
| TGCATGCAATCTAAATCCCT+TGG | - | 15122:10061-10080 | MS.gene068792:intergenic | 40.0% | |
| ! | ACAACTTGATCTGGTGGATA+TGG | - | 15122:9642-9661 | MS.gene068792:intergenic | 40.0% |
| ! | GCATCCATTGAACAAGTTGA+AGG | + | 15122:10374-10393 | MS.gene068792:CDS | 40.0% |
| ! | TCCACCAGATCAAGTTGTTA+AGG | + | 15122:9644-9663 | MS.gene068792:CDS | 40.0% |
| ! | TGGTAGTGAATCCAATTGGA+TGG | + | 15122:10485-10504 | MS.gene068792:CDS | 40.0% |
| !! | GAGTTGGTAGTGAATCCAAT+TGG | + | 15122:10481-10500 | MS.gene068792:CDS | 40.0% |
| !! | GGTGGATTGAAGTTGTATTG+GGG | + | 15122:9836-9855 | MS.gene068792:CDS | 40.0% |
| !!! | TGGAATTGTTTTTGCTGCAC+TGG | + | 15122:9710-9729 | MS.gene068792:CDS | 40.0% |
| ACAGTGAAGGAGTGATGTGT+GGG | + | 15122:10412-10431 | MS.gene068792:CDS | 45.0% | |
| ACTCCTTCACTGTCTATCCT+TGG | - | 15122:10405-10424 | MS.gene068792:intergenic | 45.0% | |
| ATGCCAAGGATAGACAGTGA+AGG | + | 15122:10399-10418 | MS.gene068792:CDS | 45.0% | |
| GTCAACAGTATTCCTCGAGT+TGG | + | 15122:10465-10484 | MS.gene068792:CDS | 45.0% | |
| TTGCTCCCATTCCATCCAAT+TGG | - | 15122:10499-10518 | MS.gene068792:intergenic | 45.0% | |
| TTGGATTCACTACCAACTCG+AGG | - | 15122:10480-10499 | MS.gene068792:intergenic | 45.0% | |
| ! | ACAAGTTGAAGGCAATGCCA+AGG | + | 15122:10385-10404 | MS.gene068792:CDS | 45.0% |
| ! | TTTATGCAGGGTTCTGATGG+TGG | + | 15122:9818-9837 | MS.gene068792:intron | 45.0% |
| !! | AGTACTGATCTCCGCCAATT+TGG | - | 15122:9377-9396 | MS.gene068792:intergenic | 45.0% |
| ACCTGCTGGAAAAGGAGCAA+AGG | - | 15122:9930-9949 | MS.gene068792:intergenic | 50.0% | |
| CAGTGAAGGAGTGATGTGTG+GGG | + | 15122:10413-10432 | MS.gene068792:CDS | 50.0% | |
| GACAGTGAAGGAGTGATGTG+TGG | + | 15122:10411-10430 | MS.gene068792:CDS | 50.0% | |
| GATCACAACAGACCCATCAG+CGG | - | 15122:9867-9886 | MS.gene068792:intergenic | 50.0% | |
| GATGTGTGGGGCCAACTTTA+AGG | + | 15122:10425-10444 | MS.gene068792:CDS | 50.0% | |
| GTGGATGAGCAAGACCTTTG+TGG | - | 15122:8867-8886 | MS.gene068792:intergenic | 50.0% | |
| TCCGAGTGGTAAGACACCAA+GGG | + | 15122:10042-10061 | MS.gene068792:intron | 50.0% | |
| ! | GATCTGGTGGATATGGACCA+CGG | - | 15122:9635-9654 | MS.gene068792:intergenic | 50.0% |
| ! | TCCTTTGCTCCTTTTCCAGC+AGG | + | 15122:9926-9945 | MS.gene068792:CDS | 50.0% |
| !! | ATTGGGGTATTGCCGCTGAT+GGG | + | 15122:9852-9871 | MS.gene068792:CDS | 50.0% |
| !! | TATTGGGGTATTGCCGCTGA+TGG | + | 15122:9851-9870 | MS.gene068792:CDS | 50.0% |
| !! | TCCCTTGGTGTCTTACCACT+CGG | - | 15122:10046-10065 | MS.gene068792:intergenic | 50.0% |
| CACTCGGACCAACCACATGT+TGG | - | 15122:10030-10049 | MS.gene068792:intergenic | 55.0% | |
| GCAGGGCTATTCAGCTCTTC+AGG | - | 15122:8889-8908 | MS.gene068792:intergenic | 55.0% | |
| GGGCTATTCAGCTCTTCAGG+TGG | - | 15122:8886-8905 | MS.gene068792:intergenic | 55.0% | |
| GTCCGAGTGGTAAGACACCA+AGG | + | 15122:10041-10060 | MS.gene068792:intron | 55.0% | |
| GTGGATATGGACCACGGTCA+CGG | - | 15122:9629-9648 | MS.gene068792:intergenic | 55.0% | |
| TATAGGACACTCCGTGACCG+TGG | + | 15122:9615-9634 | MS.gene068792:CDS | 55.0% | |
| ! | CAACATGTGGTTGGTCCGAG+TGG | + | 15122:10028-10047 | MS.gene068792:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15122 | gene | 8850 | 10524 | 8850 | ID=MS.gene068792 |
| 15122 | mRNA | 8850 | 10524 | 8850 | ID=MS.gene068792.t1;Parent=MS.gene068792 |
| 15122 | exon | 8850 | 9058 | 8850 | ID=MS.gene068792.t1.exon1;Parent=MS.gene068792.t1 |
| 15122 | CDS | 8850 | 9058 | 8850 | ID=cds.MS.gene068792.t1;Parent=MS.gene068792.t1 |
| 15122 | exon | 9491 | 9731 | 9491 | ID=MS.gene068792.t1.exon2;Parent=MS.gene068792.t1 |
| 15122 | CDS | 9491 | 9731 | 9491 | ID=cds.MS.gene068792.t1;Parent=MS.gene068792.t1 |
| 15122 | exon | 9827 | 9947 | 9827 | ID=MS.gene068792.t1.exon3;Parent=MS.gene068792.t1 |
| 15122 | CDS | 9827 | 9947 | 9827 | ID=cds.MS.gene068792.t1;Parent=MS.gene068792.t1 |
| 15122 | exon | 10334 | 10524 | 10334 | ID=MS.gene068792.t1.exon4;Parent=MS.gene068792.t1 |
| 15122 | CDS | 10334 | 10524 | 10334 | ID=cds.MS.gene068792.t1;Parent=MS.gene068792.t1 |
| Gene Sequence |
| Protein sequence |
