Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068957.t1 | XP_003628404.1 | 93 | 115 | 8 | 0 | 14 | 128 | 105 | 219 | 2.40E-56 | 228 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068957.t1 | P49332 | 60.4 | 111 | 44 | 0 | 17 | 127 | 110 | 220 | 7.2e-33 | 141.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068957.t1 | G7LB15 | 93.0 | 115 | 8 | 0 | 14 | 128 | 105 | 219 | 1.8e-56 | 228.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052665 | MS.gene068957 | 0.80257 | 5.27E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene068957 | 0.803797 | 2.94E-49 | -1.69E-46 |
| MS.gene056875 | MS.gene068957 | 0.803012 | 4.27E-49 | -1.69E-46 |
| MS.gene057469 | MS.gene068957 | 0.804268 | 2.34E-49 | -1.69E-46 |
| MS.gene059128 | MS.gene068957 | 0.819784 | 9.68E-53 | -1.69E-46 |
| MS.gene059713 | MS.gene068957 | 0.800011 | 1.76E-48 | -1.69E-46 |
| MS.gene060480 | MS.gene068957 | 0.810988 | 8.76E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068957.t1 | MTR_8g056940 | 93.043 | 115 | 8 | 0 | 14 | 128 | 105 | 219 | 3.87e-75 | 222 |
| MS.gene068957.t1 | MTR_2g070140 | 57.018 | 114 | 49 | 0 | 14 | 127 | 89 | 202 | 8.69e-41 | 134 |
| MS.gene068957.t1 | MTR_4g124130 | 58.929 | 112 | 46 | 0 | 14 | 125 | 106 | 217 | 5.68e-40 | 133 |
| MS.gene068957.t1 | MTR_3g467420 | 51.754 | 114 | 55 | 0 | 14 | 127 | 110 | 223 | 2.06e-39 | 132 |
| MS.gene068957.t1 | MTR_3g467430 | 52.632 | 114 | 54 | 0 | 14 | 127 | 110 | 223 | 2.15e-39 | 132 |
| MS.gene068957.t1 | MTR_2g070060 | 53.913 | 115 | 53 | 0 | 14 | 128 | 105 | 219 | 7.80e-39 | 130 |
| MS.gene068957.t1 | MTR_2g070180 | 56.364 | 110 | 48 | 0 | 14 | 123 | 105 | 214 | 1.11e-37 | 127 |
| MS.gene068957.t1 | MTR_2g070070 | 56.522 | 115 | 50 | 0 | 14 | 128 | 105 | 219 | 7.07e-37 | 125 |
| MS.gene068957.t1 | MTR_2g070210 | 52.727 | 110 | 52 | 0 | 14 | 123 | 107 | 216 | 2.25e-35 | 121 |
| MS.gene068957.t1 | MTR_2g070150 | 59.184 | 98 | 40 | 0 | 14 | 111 | 105 | 202 | 8.28e-35 | 119 |
| MS.gene068957.t1 | MTR_2g070110 | 62.069 | 87 | 33 | 0 | 37 | 123 | 127 | 213 | 3.02e-34 | 118 |
| MS.gene068957.t1 | MTR_2g070200 | 53.509 | 114 | 51 | 2 | 14 | 125 | 105 | 218 | 1.87e-33 | 116 |
| MS.gene068957.t1 | MTR_5g076900 | 51.818 | 110 | 53 | 0 | 14 | 123 | 107 | 216 | 3.47e-33 | 115 |
| MS.gene068957.t1 | MTR_2g070130 | 47.748 | 111 | 58 | 0 | 14 | 124 | 105 | 215 | 1.94e-32 | 114 |
| MS.gene068957.t1 | MTR_2g070120 | 44.545 | 110 | 61 | 0 | 13 | 122 | 104 | 213 | 2.50e-27 | 100 |
| MS.gene068957.t1 | MTR_4g043640 | 86.047 | 43 | 6 | 0 | 57 | 99 | 44 | 86 | 5.61e-23 | 85.9 |
| MS.gene068957.t1 | MTR_0186s0030 | 97.297 | 37 | 1 | 0 | 14 | 50 | 105 | 141 | 1.46e-17 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068957.t1 | AT1G78380 | 56.881 | 109 | 47 | 0 | 17 | 125 | 108 | 216 | 2.12e-38 | 129 |
| MS.gene068957.t1 | AT1G78360 | 56.075 | 107 | 47 | 0 | 19 | 125 | 111 | 217 | 6.68e-37 | 125 |
| MS.gene068957.t1 | AT1G78370 | 55.140 | 107 | 48 | 0 | 17 | 123 | 108 | 214 | 7.92e-37 | 125 |
| MS.gene068957.t1 | AT1G17180 | 58.654 | 104 | 43 | 0 | 22 | 125 | 113 | 216 | 1.40e-36 | 124 |
| MS.gene068957.t1 | AT1G17170 | 55.556 | 117 | 49 | 2 | 11 | 127 | 104 | 217 | 5.78e-35 | 120 |
| MS.gene068957.t1 | AT1G78340 | 56.075 | 107 | 47 | 0 | 17 | 123 | 108 | 214 | 8.09e-35 | 120 |
| MS.gene068957.t1 | AT1G53680 | 53.211 | 109 | 49 | 2 | 19 | 125 | 113 | 221 | 2.26e-33 | 116 |
| MS.gene068957.t1 | AT1G17190 | 45.872 | 109 | 59 | 0 | 17 | 125 | 109 | 217 | 8.71e-29 | 104 |
| MS.gene068957.t1 | AT1G78320 | 50.980 | 102 | 50 | 0 | 22 | 123 | 86 | 187 | 2.20e-28 | 102 |
| MS.gene068957.t1 | AT1G78320 | 50.980 | 102 | 50 | 0 | 22 | 123 | 113 | 214 | 4.86e-28 | 102 |
| MS.gene068957.t1 | AT3G43800 | 42.609 | 115 | 65 | 1 | 14 | 127 | 109 | 223 | 3.31e-24 | 93.2 |
Find 23 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAGACCAACCTTATTT+TGG | 0.247910 | 8.1:-58121676 | MS.gene068957:CDS |
| GGAGTTGATTGAAGGCTTAA+AGG | 0.261757 | 8.1:-58121713 | MS.gene068957:CDS |
| TTTCTCATCCGCAGCAGATT+TGG | 0.264315 | 8.1:+58121504 | None:intergenic |
| CTTTGATCCCTTTCTATAGT+TGG | 0.290757 | 8.1:-58121624 | MS.gene068957:CDS |
| GTGATGTTGGAGGAAGGATA+TGG | 0.346197 | 8.1:-58121771 | MS.gene068957:CDS |
| AAAGAGAGATGAGATTGAAT+TGG | 0.418834 | 8.1:-58121743 | MS.gene068957:CDS |
| AATGTGTCACCACCAAAATA+AGG | 0.439985 | 8.1:+58121664 | None:intergenic |
| TGAGATTGAATTGGCAAAGA+AGG | 0.443100 | 8.1:-58121734 | MS.gene068957:CDS |
| AAAGTGCAGGTTGGTGATGT+TGG | 0.443577 | 8.1:-58121784 | MS.gene068957:CDS |
| GCAAAGAAGGAGTTGATTGA+AGG | 0.480756 | 8.1:-58121721 | MS.gene068957:CDS |
| TGATGTTGGAGGAAGGATAT+GGG | 0.485671 | 8.1:-58121770 | MS.gene068957:CDS |
| TATAAAACCAACTATAGAAA+GGG | 0.488530 | 8.1:+58121617 | None:intergenic |
| AAGGAATTAGAGAATGTGCT+TGG | 0.497037 | 8.1:-58121694 | MS.gene068957:CDS |
| GTATAAAACCAACTATAGAA+AGG | 0.503827 | 8.1:+58121616 | None:intergenic |
| GTGTGATAAGTTGATTGCTT+GGG | 0.528271 | 8.1:-58121557 | MS.gene068957:CDS |
| ATTATGCAACTTCAAAGTTG+AGG | 0.546513 | 8.1:-58121584 | MS.gene068957:CDS |
| GTTGGAGGAAGGATATGGGC+TGG | 0.551186 | 8.1:-58121766 | MS.gene068957:CDS |
| TTGTTGTTGACTATAGGAAG+AGG | 0.553068 | 8.1:-58121468 | MS.gene068957:CDS |
| AGTGTGATAAGTTGATTGCT+TGG | 0.574483 | 8.1:-58121558 | MS.gene068957:CDS |
| GTGCAGGTTGGTGATGTTGG+AGG | 0.591902 | 8.1:-58121781 | MS.gene068957:CDS |
| TGTCACCACCAAAATAAGGT+TGG | 0.621870 | 8.1:+58121668 | None:intergenic |
| AGGTTGGTGATGTTGGAGGA+AGG | 0.642797 | 8.1:-58121777 | MS.gene068957:CDS |
| GAGTGTTTCCAAATCTGCTG+CGG | 0.660347 | 8.1:-58121512 | MS.gene068957:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTATTCATTTTAATG+TGG | - | chr8.1:58121458-58121477 | MS.gene068957:CDS | 10.0% |
| !! | TATAAAACCAACTATAGAAA+GGG | + | chr8.1:58121656-58121675 | None:intergenic | 20.0% |
| ! | GTATAAAACCAACTATAGAA+AGG | + | chr8.1:58121657-58121676 | None:intergenic | 25.0% |
| !!! | ATGATTTTGTTGTTGACTAT+AGG | - | chr8.1:58121796-58121815 | MS.gene068957:CDS | 25.0% |
| AAAGAGAGATGAGATTGAAT+TGG | - | chr8.1:58121527-58121546 | MS.gene068957:CDS | 30.0% | |
| ATTATGCAACTTCAAAGTTG+AGG | - | chr8.1:58121686-58121705 | MS.gene068957:CDS | 30.0% | |
| AAGGAATTAGAGAATGTGCT+TGG | - | chr8.1:58121576-58121595 | MS.gene068957:CDS | 35.0% | |
| AATGTGTCACCACCAAAATA+AGG | + | chr8.1:58121609-58121628 | None:intergenic | 35.0% | |
| CTTTGATCCCTTTCTATAGT+TGG | - | chr8.1:58121646-58121665 | MS.gene068957:CDS | 35.0% | |
| TGAGATTGAATTGGCAAAGA+AGG | - | chr8.1:58121536-58121555 | MS.gene068957:CDS | 35.0% | |
| ! | TAATGTGGTGAAAAAAGTGC+AGG | - | chr8.1:58121473-58121492 | MS.gene068957:CDS | 35.0% |
| ! | TTGTTGTTGACTATAGGAAG+AGG | - | chr8.1:58121802-58121821 | MS.gene068957:CDS | 35.0% |
| !! | AGTGTGATAAGTTGATTGCT+TGG | - | chr8.1:58121712-58121731 | MS.gene068957:CDS | 35.0% |
| !! | GTGTGATAAGTTGATTGCTT+GGG | - | chr8.1:58121713-58121732 | MS.gene068957:CDS | 35.0% |
| !!! | ATTTTGGTGGTGACACATTT+GGG | - | chr8.1:58121610-58121629 | MS.gene068957:CDS | 35.0% |
| !!! | TATTTTGGTGGTGACACATT+TGG | - | chr8.1:58121609-58121628 | MS.gene068957:CDS | 35.0% |
| TGATGTTGGAGGAAGGATAT+GGG | - | chr8.1:58121500-58121519 | MS.gene068957:CDS | 40.0% | |
| TGTCACCACCAAAATAAGGT+TGG | + | chr8.1:58121605-58121624 | None:intergenic | 40.0% | |
| ! | GCAAAGAAGGAGTTGATTGA+AGG | - | chr8.1:58121549-58121568 | MS.gene068957:CDS | 40.0% |
| ! | GGAGTTGATTGAAGGCTTAA+AGG | - | chr8.1:58121557-58121576 | MS.gene068957:CDS | 40.0% |
| !! | CTTGGAGACCAACCTTATTT+TGG | - | chr8.1:58121594-58121613 | MS.gene068957:CDS | 40.0% |
| GAGTGTTTCCAAATCTGCTG+CGG | - | chr8.1:58121758-58121777 | MS.gene068957:CDS | 45.0% | |
| GTGATGTTGGAGGAAGGATA+TGG | - | chr8.1:58121499-58121518 | MS.gene068957:CDS | 45.0% | |
| TTTCTCATCCGCAGCAGATT+TGG | + | chr8.1:58121769-58121788 | None:intergenic | 45.0% | |
| ! | GTGGTGAAAAAAGTGCAGGT+TGG | - | chr8.1:58121477-58121496 | MS.gene068957:CDS | 45.0% |
| !! | AAAGTGCAGGTTGGTGATGT+TGG | - | chr8.1:58121486-58121505 | MS.gene068957:CDS | 45.0% |
| !!! | GGAGACCAACCTTATTTTGG+TGG | - | chr8.1:58121597-58121616 | MS.gene068957:CDS | 45.0% |
| !! | AGGTTGGTGATGTTGGAGGA+AGG | - | chr8.1:58121493-58121512 | MS.gene068957:CDS | 50.0% |
| GTTGGAGGAAGGATATGGGC+TGG | - | chr8.1:58121504-58121523 | MS.gene068957:CDS | 55.0% | |
| !! | GTGCAGGTTGGTGATGTTGG+AGG | - | chr8.1:58121489-58121508 | MS.gene068957:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 58121453 | 58121839 | 58121453 | ID=MS.gene068957 |
| chr8.1 | mRNA | 58121453 | 58121839 | 58121453 | ID=MS.gene068957.t1;Parent=MS.gene068957 |
| chr8.1 | exon | 58121453 | 58121839 | 58121453 | ID=MS.gene068957.t1.exon1;Parent=MS.gene068957.t1 |
| chr8.1 | CDS | 58121453 | 58121839 | 58121453 | ID=cds.MS.gene068957.t1;Parent=MS.gene068957.t1 |
| Gene Sequence |
| Protein sequence |
