Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069170.t1 | XP_003517200.1 | 100 | 85 | 0 | 0 | 1 | 85 | 1 | 85 | 2.20E-37 | 164.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069170.t1 | Q94JY4 | 90.6 | 85 | 8 | 0 | 1 | 85 | 1 | 85 | 1.6e-36 | 152.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069170.t1 | A0A0B2PBB9 | 100.0 | 85 | 0 | 0 | 1 | 85 | 1 | 85 | 1.6e-37 | 164.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene069170 | 0.807296 | 5.42E-50 | -1.69E-46 |
| MS.gene050815 | MS.gene069170 | 0.802084 | 6.63E-49 | -1.69E-46 |
| MS.gene051328 | MS.gene069170 | 0.80572 | 1.16E-49 | -1.69E-46 |
| MS.gene057079 | MS.gene069170 | 0.804258 | 2.35E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069170.t1 | MTR_5g030935 | 100.000 | 85 | 0 | 0 | 1 | 85 | 1 | 85 | 2.67e-57 | 171 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069170.t1 | AT2G22640 | 90.588 | 85 | 8 | 0 | 1 | 85 | 1 | 85 | 6.83e-53 | 159 |
Find 12 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAAATTCAGTACCGAAT+TGG | 0.368507 | 5.3:+20031530 | None:intergenic |
| GAATCGCTGTTCAAGCTGAT+TGG | 0.386107 | 5.3:-20031615 | MS.gene069170:CDS |
| TGATGTATTGGAGCGTCGTT+TGG | 0.391324 | 5.3:-20031373 | MS.gene069170:CDS |
| TTCGATTTCCTTGTCCAATT+CGG | 0.396293 | 5.3:-20031544 | MS.gene069170:intron |
| GAATGAAAAGCTTGATGTAT+TGG | 0.415813 | 5.3:-20031385 | MS.gene069170:CDS |
| AATCGCTGTTCAAGCTGATT+GGG | 0.456401 | 5.3:-20031614 | MS.gene069170:CDS |
| AGTTCAAGTTGGAAATGCTT+CGG | 0.460394 | 5.3:-20031340 | MS.gene069170:CDS |
| ATTCCATCAATGGCACGTGC+TGG | 0.501604 | 5.3:-20031664 | None:intergenic |
| GAGTTACTTGAAGTTCAAGT+TGG | 0.543162 | 5.3:-20031351 | MS.gene069170:CDS |
| CCATCAATGGCACGTGCTGG+AGG | 0.587599 | 5.3:-20031661 | None:intergenic |
| ATTCAGTACCGAATTGGACA+AGG | 0.614288 | 5.3:+20031536 | None:intergenic |
| ATCACAAACGCAGTTAACGT+TGG | 0.624596 | 5.3:-20031637 | MS.gene069170:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAAGATTTCAAATAACA+AGG | + | chr5.3:20031495-20031514 | None:intergenic | 15.0% |
| !!! | AATCTTATATCTTTTTTTGA+TGG | - | chr5.3:20031505-20031524 | MS.gene069170:intron | 15.0% |
| !!! | ATCTTATATCTTTTTTTGAT+GGG | - | chr5.3:20031506-20031525 | MS.gene069170:intron | 15.0% |
| !!! | TGTTATTTTTGTTATTTCAG+AGG | - | chr5.3:20031533-20031552 | MS.gene069170:intron | 20.0% |
| ! | ATTTCAAATAACAAGGCAAA+AGG | + | chr5.3:20031488-20031507 | None:intergenic | 25.0% |
| ! | TTTCAAATAACAAGGCAAAA+GGG | + | chr5.3:20031487-20031506 | None:intergenic | 25.0% |
| GAATGAAAAGCTTGATGTAT+TGG | - | chr5.3:20031584-20031603 | MS.gene069170:CDS | 30.0% | |
| GAGTTACTTGAAGTTCAAGT+TGG | - | chr5.3:20031618-20031637 | MS.gene069170:CDS | 35.0% | |
| TTCGATTTCCTTGTCCAATT+CGG | - | chr5.3:20031425-20031444 | MS.gene069170:intron | 35.0% | |
| ! | GAAGAAATTCAGTACCGAAT+TGG | + | chr5.3:20031442-20031461 | None:intergenic | 35.0% |
| !! | AGTTCAAGTTGGAAATGCTT+CGG | - | chr5.3:20031629-20031648 | MS.gene069170:CDS | 35.0% |
| AATCGCTGTTCAAGCTGATT+GGG | - | chr5.3:20031355-20031374 | MS.gene069170:CDS | 40.0% | |
| ATCACAAACGCAGTTAACGT+TGG | - | chr5.3:20031332-20031351 | MS.gene069170:CDS | 40.0% | |
| ! | ATTCAGTACCGAATTGGACA+AGG | + | chr5.3:20031436-20031455 | None:intergenic | 40.0% |
| GAATCGCTGTTCAAGCTGAT+TGG | - | chr5.3:20031354-20031373 | MS.gene069170:CDS | 45.0% | |
| !! | TGATGTATTGGAGCGTCGTT+TGG | - | chr5.3:20031596-20031615 | MS.gene069170:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 20031314 | 20031677 | 20031314 | ID=MS.gene069170 |
| chr5.3 | mRNA | 20031314 | 20031677 | 20031314 | ID=MS.gene069170.t1;Parent=MS.gene069170 |
| chr5.3 | exon | 20031545 | 20031677 | 20031545 | ID=MS.gene069170.t1.exon1;Parent=MS.gene069170.t1 |
| chr5.3 | CDS | 20031545 | 20031677 | 20031545 | ID=cds.MS.gene069170.t1;Parent=MS.gene069170.t1 |
| chr5.3 | exon | 20031314 | 20031438 | 20031314 | ID=MS.gene069170.t1.exon2;Parent=MS.gene069170.t1 |
| chr5.3 | CDS | 20031314 | 20031438 | 20031314 | ID=cds.MS.gene069170.t1;Parent=MS.gene069170.t1 |
| Gene Sequence |
| Protein sequence |
