AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057079


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057079.t1 MTR_5g091310 95.652 69 3 0 1 69 18 86 1.87e-44 137
MS.gene057079.t1 MTR_4g094635 50.685 73 29 3 1 69 21 90 3.30e-16 66.6
MS.gene057079.t1 MTR_4g094630 50.000 72 28 3 3 69 6 74 2.10e-15 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057079.t1 AT5G46295 60.465 43 16 1 27 69 30 71 2.84e-13 58.5
MS.gene057079.t1 AT1G06475 55.556 45 19 1 26 69 49 93 4.37e-12 56.2

Find 10 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAGTGAAGAAAGAAATT+TGG 0.267366 5.3:-73354928 MS.gene057079:CDS
CATGGAGATTCTCTGCGTTT+AGG 0.350104 5.3:-73354904 MS.gene057079:CDS
TCGTGGATACTGTTGTGTTT+AGG 0.353121 5.3:-73354850 MS.gene057079:CDS
GAAAGAAGAGTAGCATGTTA+TGG 0.462290 5.3:-73354955 MS.gene057079:CDS
TGCTTCTTCTATCTTTGTTG+TGG 0.470772 5.3:-73354785 MS.gene057079:CDS
TCATCTTCAAATGACATTCG+TGG 0.492578 5.3:-73354867 MS.gene057079:CDS
GGAGATTCTCTGCGTTTAGG+TGG 0.556332 5.3:-73354901 MS.gene057079:CDS
TGAAGAAAGAAATTTGGCCA+TGG 0.584343 5.3:-73354922 MS.gene057079:CDS
AAGATAGAAGAAGCAAACAG+TGG 0.638854 5.3:+73354793 None:intergenic
CTAAACGCAGAGAATCTCCA+TGG 0.659319 5.3:+73354905 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAAGTGAAGAAAGAAATT+TGG - chr5.3:73354803-73354822 MS.gene057079:CDS 25.0%
AAGATAGAAGAAGCAAACAG+TGG + chr5.3:73354941-73354960 None:intergenic 35.0%
GAAAGAAGAGTAGCATGTTA+TGG - chr5.3:73354776-73354795 MS.gene057079:CDS 35.0%
TCATCTTCAAATGACATTCG+TGG - chr5.3:73354864-73354883 MS.gene057079:CDS 35.0%
TGAAGAAAGAAATTTGGCCA+TGG - chr5.3:73354809-73354828 MS.gene057079:CDS 35.0%
TGCTTCTTCTATCTTTGTTG+TGG - chr5.3:73354946-73354965 MS.gene057079:CDS 35.0%
TCGTGGATACTGTTGTGTTT+AGG - chr5.3:73354881-73354900 MS.gene057079:CDS 40.0%
CATGGAGATTCTCTGCGTTT+AGG - chr5.3:73354827-73354846 MS.gene057079:CDS 45.0%
CTAAACGCAGAGAATCTCCA+TGG + chr5.3:73354829-73354848 None:intergenic 45.0%
GGAGATTCTCTGCGTTTAGG+TGG - chr5.3:73354830-73354849 MS.gene057079:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 73354772 73354981 73354772 ID=MS.gene057079
chr5.3 mRNA 73354772 73354981 73354772 ID=MS.gene057079.t1;Parent=MS.gene057079
chr5.3 exon 73354772 73354981 73354772 ID=MS.gene057079.t1.exon1;Parent=MS.gene057079.t1
chr5.3 CDS 73354772 73354981 73354772 ID=cds.MS.gene057079.t1;Parent=MS.gene057079.t1
Gene Sequence

>MS.gene057079

ATGGGAAAGAAGAGTAGCATGTTATGGTTGAAGAAAGTGAAGAAAGAAATTTGGCCATGGAGATTCTCTGCGTTTAGGTGGAAACGACTTGATCATCTTCAAATGACATTCGTGGATACTGTTGTGTTTAGGATACTCTCTGTTGCTGAAGCTGTTGTTCTTGTCTCCACTGTTTGCTTCTTCTATCTTTGTTGTGGCTGCAATTTCTGA

Protein sequence

>MS.gene057079.t1

MGKKSSMLWLKKVKKEIWPWRFSAFRWKRLDHLQMTFVDTVVFRILSVAEAVVLVSTVCFFYLCCGCNF