Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069368.t1 | XP_013464732.1 | 97.6 | 253 | 6 | 0 | 1 | 253 | 1 | 253 | 1.60E-144 | 521.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069368.t1 | P24805 | 40.9 | 149 | 87 | 1 | 1 | 149 | 1 | 148 | 3.1e-27 | 123.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069368.t1 | B7FHV5 | 97.6 | 253 | 6 | 0 | 1 | 253 | 1 | 253 | 1.2e-144 | 521.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052166 | MS.gene069368 | 0.810854 | 9.37E-51 | -1.69E-46 |
| MS.gene056623 | MS.gene069368 | 0.852006 | 6.13E-61 | -1.69E-46 |
| MS.gene059856 | MS.gene069368 | 0.950653 | 1.05E-108 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069368.t1 | MTR_2g079670 | 97.628 | 253 | 6 | 0 | 1 | 253 | 1 | 253 | 0.0 | 516 |
| MS.gene069368.t1 | MTR_2g079670 | 96.859 | 191 | 6 | 0 | 1 | 191 | 1 | 191 | 2.90e-137 | 384 |
| MS.gene069368.t1 | MTR_2g079670 | 98.361 | 183 | 3 | 0 | 71 | 253 | 22 | 204 | 5.19e-135 | 379 |
| MS.gene069368.t1 | MTR_2g013140 | 66.000 | 250 | 84 | 1 | 1 | 250 | 1 | 249 | 2.50e-117 | 336 |
| MS.gene069368.t1 | MTR_4g088955 | 60.956 | 251 | 97 | 1 | 1 | 250 | 64 | 314 | 7.38e-107 | 311 |
| MS.gene069368.t1 | MTR_2g013140 | 64.398 | 191 | 67 | 1 | 1 | 191 | 1 | 190 | 2.13e-81 | 242 |
| MS.gene069368.t1 | MTR_1g041380 | 47.012 | 251 | 124 | 4 | 1 | 247 | 1 | 246 | 4.30e-77 | 234 |
| MS.gene069368.t1 | MTR_1g102680 | 47.222 | 252 | 122 | 5 | 1 | 247 | 1 | 246 | 1.41e-76 | 232 |
| MS.gene069368.t1 | MTR_2g022520 | 41.631 | 233 | 127 | 6 | 1 | 230 | 1 | 227 | 4.39e-55 | 177 |
| MS.gene069368.t1 | MTR_4g117050 | 39.056 | 233 | 134 | 6 | 1 | 230 | 1 | 228 | 7.88e-54 | 174 |
| MS.gene069368.t1 | MTR_1g041380 | 44.560 | 193 | 100 | 3 | 1 | 191 | 1 | 188 | 1.27e-51 | 167 |
| MS.gene069368.t1 | MTR_4g117050 | 44.242 | 165 | 89 | 2 | 67 | 230 | 21 | 183 | 3.60e-47 | 155 |
| MS.gene069368.t1 | MTR_1g019625 | 75.926 | 54 | 6 | 1 | 113 | 159 | 14 | 67 | 3.26e-19 | 79.3 |
| MS.gene069368.t1 | MTR_7g059340 | 76.316 | 38 | 9 | 0 | 114 | 151 | 18 | 55 | 1.06e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069368.t1 | AT4G27450 | 78.629 | 248 | 52 | 1 | 1 | 247 | 1 | 248 | 3.65e-147 | 411 |
| MS.gene069368.t1 | AT3G15450 | 68.675 | 249 | 74 | 3 | 1 | 247 | 1 | 247 | 3.06e-125 | 356 |
| MS.gene069368.t1 | AT3G15450 | 66.839 | 193 | 60 | 3 | 1 | 191 | 1 | 191 | 4.51e-89 | 263 |
| MS.gene069368.t1 | AT5G43830 | 48.810 | 252 | 119 | 4 | 1 | 247 | 1 | 247 | 5.78e-80 | 241 |
| MS.gene069368.t1 | AT3G15450 | 64.286 | 182 | 58 | 4 | 1 | 180 | 1 | 177 | 2.40e-75 | 227 |
| MS.gene069368.t1 | AT3G22850 | 44.939 | 247 | 133 | 1 | 1 | 247 | 1 | 244 | 6.30e-74 | 226 |
| MS.gene069368.t1 | AT5G19140 | 42.241 | 232 | 127 | 5 | 1 | 230 | 1 | 227 | 2.92e-56 | 180 |
| MS.gene069368.t1 | AT5G19140 | 48.521 | 169 | 84 | 2 | 63 | 230 | 25 | 191 | 4.18e-51 | 166 |
| MS.gene069368.t1 | AT5G19140 | 39.655 | 232 | 121 | 6 | 1 | 230 | 1 | 215 | 2.42e-48 | 159 |
Find 0 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTTTTAACTCTAAATA+CGG | - | 15114:10462-10481 | MS.gene069368:intergenic | 10.0% |
| !!! | ATCAATTTTTCCAATAAAAA+AGG | - | 15114:9646-9665 | MS.gene069368:intergenic | 15.0% |
| !!! | ATTTTGTTTTCAAGATAAAT+GGG | - | 15114:10278-10297 | MS.gene069368:intergenic | 15.0% |
| !! | AAAACAAAATCTCTTCAATT+TGG | + | 15114:10288-10307 | MS.gene069368:intron | 20.0% |
| !! | TCCAACATAAAAGTTTAATA+CGG | + | 15114:9557-9576 | MS.gene069368:intron | 20.0% |
| !! | TTTGTTAGATCAATATGATA+CGG | + | 15114:10421-10440 | MS.gene069368:intron | 20.0% |
| !!! | CATCTTTAATTCCTTTTATA+AGG | + | 15114:9524-9543 | MS.gene069368:intron | 20.0% |
| !!! | GATTTTGTTTTCAAGATAAA+TGG | - | 15114:10279-10298 | MS.gene069368:intergenic | 20.0% |
| !!! | TTTTAGTACACCTTTTTTTA+TGG | + | 15114:9398-9417 | MS.gene069368:intron | 20.0% |
| ! | AATACTTGTTCTATGAACTT+TGG | + | 15114:9282-9301 | MS.gene069368:CDS | 25.0% |
| ! | AGAATTGTTAACAAAGAACA+TGG | - | 15114:10062-10081 | MS.gene069368:intergenic | 25.0% |
| ! | TTCATAGAACAAGTATTATC+AGG | - | 15114:9279-9298 | MS.gene069368:intergenic | 25.0% |
| !! | ACCGTATTAAACTTTTATGT+TGG | - | 15114:9561-9580 | MS.gene069368:intergenic | 25.0% |
| !! | TCCTTTTATAAGGATGAATT+CGG | + | 15114:9534-9553 | MS.gene069368:intron | 25.0% |
| !!! | ATGATGTTTGTTTTTTATGC+AGG | + | 15114:10105-10124 | MS.gene069368:intron | 25.0% |
| !!! | TGATGTTTGTTTTTTATGCA+GGG | + | 15114:10106-10125 | MS.gene069368:intron | 25.0% |
| !!! | TTTTGAAATCATTGAATGCA+GGG | + | 15114:10613-10632 | MS.gene069368:intron | 25.0% |
| !!! | TTTTTGAAATCATTGAATGC+AGG | + | 15114:10612-10631 | MS.gene069368:intron | 25.0% |
| AACCTTTGATGAACAGAAAA+AGG | - | 15114:9342-9361 | MS.gene069367:intergenic | 30.0% | |
| ACATATACTGCTTATTCTTG+GGG | + | 15114:9811-9830 | MS.gene069368:CDS | 30.0% | |
| ACCGAATTCATCCTTATAAA+AGG | - | 15114:9538-9557 | MS.gene069368:intergenic | 30.0% | |
| AGAATTTCTTCAGAAAGCTT+AGG | - | 15114:9234-9253 | MS.gene069368:intergenic | 30.0% | |
| ATAAGCTTCAATCAGAAACA+TGG | - | 15114:9898-9917 | MS.gene069368:intergenic | 30.0% | |
| ATGTTATTAGTGCTAAGAGA+TGG | + | 15114:9757-9776 | MS.gene069368:intron | 30.0% | |
| ATTACATCATAACCAACATG+TGG | + | 15114:10315-10334 | MS.gene069368:intron | 30.0% | |
| ATTGATGTTACTGACCAAAT+TGG | + | 15114:9660-9679 | MS.gene069368:intron | 30.0% | |
| CAAAGACGTGTATTATGTTA+CGG | + | 15114:9601-9620 | MS.gene069368:intron | 30.0% | |
| CACTTCAAACAAATTGTCAA+AGG | + | 15114:10578-10597 | MS.gene069368:intron | 30.0% | |
| GAACTTATCAACCTTAAAGT+TGG | - | 15114:10739-10758 | MS.gene069368:intergenic | 30.0% | |
| GACATATACTGCTTATTCTT+GGG | + | 15114:9810-9829 | MS.gene069368:CDS | 30.0% | |
| TAGATCAATATGATACGGTT+TGG | + | 15114:10426-10445 | MS.gene069368:intron | 30.0% | |
| TGACATATACTGCTTATTCT+TGG | + | 15114:9809-9828 | MS.gene069368:CDS | 30.0% | |
| TTCGTAGCAACCATAAAAAA+AGG | - | 15114:9411-9430 | MS.gene069368:intergenic | 30.0% | |
| ! | AAGCAATATGGTTTGTCAAA+GGG | + | 15114:9861-9880 | MS.gene069368:CDS | 30.0% |
| ! | TGTTTCTGATTGAAGCTTAT+AGG | + | 15114:9898-9917 | MS.gene069368:CDS | 30.0% |
| ! | TTTTGTAGCAACCATGTTAT+TGG | - | 15114:9718-9737 | MS.gene069368:intergenic | 30.0% |
| !! | ATTTTTGCAGGTTGTTTTGT+GGG | + | 15114:9781-9800 | MS.gene069368:intron | 30.0% |
| !! | TATTTTTGCAGGTTGTTTTG+TGG | + | 15114:9780-9799 | MS.gene069368:intron | 30.0% |
| !!! | CAGTTGCATACCTTTTTTAT+TGG | + | 15114:9633-9652 | MS.gene069368:intron | 30.0% |
| AACCCTCTTGTACAACATTA+AGG | - | 15114:10198-10217 | MS.gene069367:intergenic | 35.0% | |
| AAGTGTCTGAGTCTCTAATT+TGG | - | 15114:10563-10582 | MS.gene069367:intergenic | 35.0% | |
| AATGATTGTTGGATCGAGAT+CGG | - | 15114:10503-10522 | MS.gene069368:intergenic | 35.0% | |
| ACCATATTGCTTGTTCAGTA+AGG | - | 15114:9853-9872 | MS.gene069368:intergenic | 35.0% | |
| AGTTCCTTAACAACTTGATC+TGG | - | 15114:9951-9970 | MS.gene069368:intergenic | 35.0% | |
| CAAAGAATCGCAATCATATG+TGG | + | 15114:9476-9495 | MS.gene069368:intron | 35.0% | |
| CAAGTTGTTAAGGAACTTGA+TGG | + | 15114:9954-9973 | MS.gene069368:CDS | 35.0% | |
| CGTAACATAATACACGTCTT+TGG | - | 15114:9603-9622 | MS.gene069368:intergenic | 35.0% | |
| GACCTTAATGTTGTACAAGA+GGG | + | 15114:10193-10212 | MS.gene069368:CDS | 35.0% | |
| GCTAACACATCAATGATTGT+TGG | - | 15114:10514-10533 | MS.gene069368:intergenic | 35.0% | |
| GTTGTTTATGACAGCACATA+TGG | + | 15114:9990-10009 | MS.gene069368:CDS | 35.0% | |
| TCCTTACTGAACAAGCAATA+TGG | + | 15114:9849-9868 | MS.gene069368:CDS | 35.0% | |
| TGAACAGAAAAAGGCTTATC+AGG | - | 15114:9333-9352 | MS.gene069368:intergenic | 35.0% | |
| TGAATCCAATTGGATGGAAT+GGG | + | 15114:10791-10810 | MS.gene069368:CDS | 35.0% | |
| TGACCTTAATGTTGTACAAG+AGG | + | 15114:10192-10211 | MS.gene069368:CDS | 35.0% | |
| TGTATGTTTCATAGTGAAGG+AGG | + | 15114:10636-10655 | MS.gene069368:CDS | 35.0% | |
| TGTGCTGTCATAAACAACAA+AGG | - | 15114:9988-10007 | MS.gene069368:intergenic | 35.0% | |
| TTAAGCCTTTGTATGATGCA+GGG | - | 15114:9206-9225 | MS.gene069368:intergenic | 35.0% | |
| TTTAAGCCTTTGTATGATGC+AGG | - | 15114:9207-9226 | MS.gene069368:intergenic | 35.0% | |
| TTTAATACGGTATCAGAGTC+TGG | + | 15114:9570-9589 | MS.gene069368:intron | 35.0% | |
| ! | AGCCTTTTTCTGTTCATCAA+AGG | + | 15114:9337-9356 | MS.gene069368:CDS | 35.0% |
| ! | ATGTTGGCAATTTTCCACAA+AGG | + | 15114:9150-9169 | MS.gene069368:CDS | 35.0% |
| ! | ATTGCCTTCAACTTGTTCAA+TGG | - | 15114:10681-10700 | MS.gene069368:intergenic | 35.0% |
| ! | CAAGCAATATGGTTTGTCAA+AGG | + | 15114:9860-9879 | MS.gene069368:CDS | 35.0% |
| !! | ATGGTGGATTGAAGTTGTAT+TGG | + | 15114:10134-10153 | MS.gene069368:CDS | 35.0% |
| !! | CTAAGAGATGGTATTTTTGC+AGG | + | 15114:9769-9788 | MS.gene069368:intron | 35.0% |
| !! | TGGTGGATTGAAGTTGTATT+GGG | + | 15114:10135-10154 | MS.gene069368:CDS | 35.0% |
| !!! | TTTTTTATGCAGGGTTCTGA+TGG | + | 15114:10115-10134 | MS.gene069368:intron | 35.0% |
| AATAGCCCTGCATCATACAA+AGG | + | 15114:9198-9217 | MS.gene069368:CDS | 40.0% | |
| ATTTGCTTGCGCCAATAACA+TGG | + | 15114:9704-9723 | MS.gene069368:intron | 40.0% | |
| CAATTGATACCTGCTGGAAA+AGG | - | 15114:10238-10257 | MS.gene069368:intergenic | 40.0% | |
| CATCATAACCAACATGTGGT+TGG | + | 15114:10319-10338 | MS.gene069368:intron | 40.0% | |
| GATGTTACTGACCAAATTGG+CGG | + | 15114:9663-9682 | MS.gene069368:intron | 40.0% | |
| GCTAAACAATTGATACCTGC+TGG | - | 15114:10244-10263 | MS.gene069368:intergenic | 40.0% | |
| GGGTGTATGTTTCATAGTGA+AGG | + | 15114:10633-10652 | MS.gene069368:intron | 40.0% | |
| GTGAATCCAATTGGATGGAA+TGG | + | 15114:10790-10809 | MS.gene069368:CDS | 40.0% | |
| TCCTTAACAACTTGATCTGG+TGG | - | 15114:9948-9967 | MS.gene069368:intergenic | 40.0% | |
| TGCATGCAATCTAAATCCCT+TGG | - | 15114:10361-10380 | MS.gene069368:intergenic | 40.0% | |
| ! | ACAACTTGATCTGGTGGATA+TGG | - | 15114:9942-9961 | MS.gene069368:intergenic | 40.0% |
| ! | GCATCCATTGAACAAGTTGA+AGG | + | 15114:10674-10693 | MS.gene069368:CDS | 40.0% |
| ! | TCCACCAGATCAAGTTGTTA+AGG | + | 15114:9944-9963 | MS.gene069368:CDS | 40.0% |
| ! | TGGTAGTGAATCCAATTGGA+TGG | + | 15114:10785-10804 | MS.gene069368:CDS | 40.0% |
| !! | GAGTTGGTAGTGAATCCAAT+TGG | + | 15114:10781-10800 | MS.gene069368:CDS | 40.0% |
| !! | GGTGGATTGAAGTTGTATTG+GGG | + | 15114:10136-10155 | MS.gene069368:CDS | 40.0% |
| !!! | TGGAATTGTTTTTGCTGCAC+TGG | + | 15114:10010-10029 | MS.gene069368:CDS | 40.0% |
| ACAGTGAAGGAGTGATGTGT+GGG | + | 15114:10712-10731 | MS.gene069368:CDS | 45.0% | |
| ACTCCTTCACTGTCTATCCT+TGG | - | 15114:10705-10724 | MS.gene069368:intergenic | 45.0% | |
| ATGCCAAGGATAGACAGTGA+AGG | + | 15114:10699-10718 | MS.gene069368:CDS | 45.0% | |
| GTCAACAGTATTCCTCGAGT+TGG | + | 15114:10765-10784 | MS.gene069368:CDS | 45.0% | |
| TTGCTCCCATTCCATCCAAT+TGG | - | 15114:10799-10818 | MS.gene069368:intergenic | 45.0% | |
| TTGGATTCACTACCAACTCG+AGG | - | 15114:10780-10799 | MS.gene069368:intergenic | 45.0% | |
| ! | ACAAGTTGAAGGCAATGCCA+AGG | + | 15114:10685-10704 | MS.gene069368:CDS | 45.0% |
| ! | TTTATGCAGGGTTCTGATGG+TGG | + | 15114:10118-10137 | MS.gene069368:intron | 45.0% |
| !! | AGTACTGATCTCCGCCAATT+TGG | - | 15114:9677-9696 | MS.gene069368:intergenic | 45.0% |
| ACCTGCTGGAAAAGGAGCAA+AGG | - | 15114:10230-10249 | MS.gene069368:intergenic | 50.0% | |
| CAGTGAAGGAGTGATGTGTG+GGG | + | 15114:10713-10732 | MS.gene069368:CDS | 50.0% | |
| GACAGTGAAGGAGTGATGTG+TGG | + | 15114:10711-10730 | MS.gene069368:CDS | 50.0% | |
| GATCACAACAGACCCATCAG+CGG | - | 15114:10167-10186 | MS.gene069368:intergenic | 50.0% | |
| GATGTGTGGGGCCAACTTTA+AGG | + | 15114:10725-10744 | MS.gene069368:CDS | 50.0% | |
| GTGGATGAGCAAGACCTTTG+TGG | - | 15114:9167-9186 | MS.gene069368:intergenic | 50.0% | |
| TCCGAGTGGTAAGACACCAA+GGG | + | 15114:10342-10361 | MS.gene069368:intron | 50.0% | |
| ! | GATCTGGTGGATATGGACCA+CGG | - | 15114:9935-9954 | MS.gene069368:intergenic | 50.0% |
| ! | TCCTTTGCTCCTTTTCCAGC+AGG | + | 15114:10226-10245 | MS.gene069368:CDS | 50.0% |
| !! | ATTGGGGTATTGCCGCTGAT+GGG | + | 15114:10152-10171 | MS.gene069368:CDS | 50.0% |
| !! | TATTGGGGTATTGCCGCTGA+TGG | + | 15114:10151-10170 | MS.gene069368:CDS | 50.0% |
| !! | TCCCTTGGTGTCTTACCACT+CGG | - | 15114:10346-10365 | MS.gene069368:intergenic | 50.0% |
| CACTCGGACCAACCACATGT+TGG | - | 15114:10330-10349 | MS.gene069368:intergenic | 55.0% | |
| GCAGGGCTATTCAGCTCTTC+AGG | - | 15114:9189-9208 | MS.gene069368:intergenic | 55.0% | |
| GGGCTATTCAGCTCTTCAGG+TGG | - | 15114:9186-9205 | MS.gene069368:intergenic | 55.0% | |
| GTCCGAGTGGTAAGACACCA+AGG | + | 15114:10341-10360 | MS.gene069368:intron | 55.0% | |
| GTGGATATGGACCACGGTCA+CGG | - | 15114:9929-9948 | MS.gene069368:intergenic | 55.0% | |
| TATAGGACACTCCGTGACCG+TGG | + | 15114:9915-9934 | MS.gene069368:CDS | 55.0% | |
| ! | CAACATGTGGTTGGTCCGAG+TGG | + | 15114:10328-10347 | MS.gene069368:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15114 | gene | 9150 | 10824 | 9150 | ID=MS.gene069368 |
| 15114 | mRNA | 9150 | 10824 | 9150 | ID=MS.gene069368.t1;Parent=MS.gene069368 |
| 15114 | exon | 9150 | 9358 | 9150 | ID=MS.gene069368.t1.exon1;Parent=MS.gene069368.t1 |
| 15114 | CDS | 9150 | 9358 | 9150 | ID=cds.MS.gene069368.t1;Parent=MS.gene069368.t1 |
| 15114 | exon | 9791 | 10031 | 9791 | ID=MS.gene069368.t1.exon2;Parent=MS.gene069368.t1 |
| 15114 | CDS | 9791 | 10031 | 9791 | ID=cds.MS.gene069368.t1;Parent=MS.gene069368.t1 |
| 15114 | exon | 10127 | 10247 | 10127 | ID=MS.gene069368.t1.exon3;Parent=MS.gene069368.t1 |
| 15114 | CDS | 10127 | 10247 | 10127 | ID=cds.MS.gene069368.t1;Parent=MS.gene069368.t1 |
| 15114 | exon | 10634 | 10824 | 10634 | ID=MS.gene069368.t1.exon4;Parent=MS.gene069368.t1 |
| 15114 | CDS | 10634 | 10824 | 10634 | ID=cds.MS.gene069368.t1;Parent=MS.gene069368.t1 |
| Gene Sequence |
| Protein sequence |
