Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070472.t1 | RHN51483.1 | 90 | 279 | 17 | 1 | 1 | 268 | 340 | 618 | 7.20E-143 | 516.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070472.t1 | A0A396HFX6 | 90.0 | 279 | 17 | 1 | 1 | 268 | 340 | 618 | 5.2e-143 | 516.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056168 | MS.gene070472 | 0.805965 | 1.03E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene070472 | 0.81763 | 2.98E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070472.t1 | MTR_0002s1270 | 84.797 | 296 | 17 | 1 | 1 | 268 | 105 | 400 | 0.0 | 516 |
| MS.gene070472.t1 | MTR_7g046380 | 84.211 | 285 | 17 | 1 | 1 | 257 | 113 | 397 | 1.90e-177 | 494 |
| MS.gene070472.t1 | MTR_5g077900 | 81.416 | 226 | 14 | 1 | 71 | 268 | 1 | 226 | 1.64e-134 | 379 |
| MS.gene070472.t1 | MTR_4g066310 | 71.429 | 105 | 24 | 1 | 1 | 99 | 81 | 185 | 6.27e-47 | 155 |
| MS.gene070472.t1 | MTR_0057s0200 | 96.610 | 59 | 2 | 0 | 210 | 268 | 1 | 59 | 3.10e-37 | 126 |
| MS.gene070472.t1 | MTR_2g092960 | 32.489 | 237 | 128 | 5 | 57 | 265 | 984 | 1216 | 3.09e-34 | 131 |
| MS.gene070472.t1 | MTR_4g066320 | 86.885 | 61 | 7 | 1 | 207 | 266 | 59 | 119 | 1.20e-33 | 119 |
| MS.gene070472.t1 | MTR_4g066320 | 86.957 | 46 | 6 | 0 | 1 | 46 | 1 | 46 | 1.86e-20 | 84.7 |
| MS.gene070472.t1 | MTR_0462s0020 | 35.057 | 174 | 107 | 2 | 93 | 264 | 122 | 291 | 1.66e-32 | 121 |
| MS.gene070472.t1 | MTR_8g017000 | 35.196 | 179 | 109 | 3 | 93 | 268 | 515 | 689 | 4.80e-28 | 113 |
| MS.gene070472.t1 | MTR_2g037270 | 35.196 | 179 | 109 | 3 | 93 | 268 | 515 | 689 | 6.91e-28 | 112 |
| MS.gene070472.t1 | MTR_3g011560 | 31.073 | 177 | 106 | 3 | 93 | 268 | 515 | 676 | 5.80e-24 | 101 |
| MS.gene070472.t1 | MTR_3g028430 | 74.074 | 54 | 5 | 1 | 215 | 268 | 15 | 59 | 8.44e-20 | 80.9 |
| MS.gene070472.t1 | MTR_8g051620 | 51.667 | 60 | 29 | 0 | 92 | 151 | 50 | 109 | 2.15e-15 | 71.2 |
| MS.gene070472.t1 | MTR_8g051620 | 54.386 | 57 | 26 | 0 | 92 | 148 | 50 | 106 | 2.22e-15 | 70.9 |
| MS.gene070472.t1 | MTR_4g066300 | 88.235 | 34 | 4 | 0 | 206 | 239 | 5 | 38 | 3.58e-14 | 66.2 |
| MS.gene070472.t1 | MTR_2g086200 | 62.500 | 48 | 18 | 0 | 219 | 266 | 185 | 232 | 6.23e-13 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGAAAGTGTTTGGGATTT+TGG | 0.107550 | 8.4:+44931805 | MS.gene070472:CDS |
| GTTGATGCAACAAGCATTTC+TGG | 0.171896 | 8.4:+44931348 | MS.gene070472:CDS |
| CTTCATTTGTTTGCAAGGTT+TGG | 0.194841 | 8.4:-44931396 | None:intergenic |
| TCTGGATAAACTTAAGACTA+TGG | 0.231009 | 8.4:+44931366 | MS.gene070472:CDS |
| TTGCCATTAGGTTGCTTTCT+TGG | 0.248489 | 8.4:-44932393 | None:intergenic |
| TTCATTTGTTTGCAAGGTTT+GGG | 0.264022 | 8.4:-44931395 | None:intergenic |
| AGAAATAGATCGAAAGTGTT+TGG | 0.315223 | 8.4:+44931797 | MS.gene070472:CDS |
| GGATTAAAATCCAAGCATAC+TGG | 0.319311 | 8.4:-44931830 | None:intergenic |
| TCATTGCTTCATTAAGATCT+TGG | 0.330442 | 8.4:-44932096 | None:intergenic |
| TGGTTAACACAGAGCTTTAG+AGG | 0.331097 | 8.4:+44932215 | MS.gene070472:intron |
| GCAATAGAAAGAAGGCTAAA+TGG | 0.337305 | 8.4:+44932256 | MS.gene070472:CDS |
| GAAATAGATCGAAAGTGTTT+GGG | 0.378893 | 8.4:+44931798 | MS.gene070472:CDS |
| AGAGGTTCATCAACTTGCTC+AGG | 0.406730 | 8.4:+44932233 | MS.gene070472:CDS |
| GTATTTGGCTCATGTTCTAA+TGG | 0.408074 | 8.4:-44931574 | None:intergenic |
| GACAACTAAACTATTGTCCT+TGG | 0.409668 | 8.4:-44932059 | None:intergenic |
| AATTTCTTCATTTGTTTGCA+AGG | 0.411399 | 8.4:-44931401 | None:intergenic |
| TATCCACAATCATTGCCATT+AGG | 0.411955 | 8.4:-44932405 | None:intergenic |
| TATACTTACTTAAATAGATA+TGG | 0.415179 | 8.4:-44931935 | None:intergenic |
| TCTTGACTTGTATAACGCTT+AGG | 0.431501 | 8.4:+44932660 | MS.gene070472:CDS |
| TAGTACTAAAACTAAAGTTC+AGG | 0.451652 | 8.4:+44931862 | MS.gene070472:CDS |
| GTTGTGCATGGAAGCACAAA+AGG | 0.453705 | 8.4:+44931448 | MS.gene070472:CDS |
| CTGGATAAACTTAAGACTAT+GGG | 0.467740 | 8.4:+44931367 | MS.gene070472:CDS |
| CTGCTAATATTACTAAGTCT+TGG | 0.472781 | 8.4:+44932460 | MS.gene070472:CDS |
| GTATGATTTGCGAAACCAAT+GGG | 0.494818 | 8.4:+44932627 | MS.gene070472:CDS |
| TAGCCAAGAAAGCAACCTAA+TGG | 0.495668 | 8.4:+44932390 | MS.gene070472:intron |
| TCATCTTCAGTTAATGTCGC+TGG | 0.495751 | 8.4:-44932601 | None:intergenic |
| CAACCTAATGGCAATGATTG+TGG | 0.497368 | 8.4:+44932402 | MS.gene070472:CDS |
| CAAGAAAACAAGTTGCCCAT+TGG | 0.504204 | 8.4:-44932642 | None:intergenic |
| GTTAATAATTATTTGTCCCA+AGG | 0.509187 | 8.4:+44932042 | MS.gene070472:CDS |
| TTCAGGAACACATGCAAACT+AGG | 0.517238 | 8.4:+44931879 | MS.gene070472:CDS |
| ACAACTAAACTATTGTCCTT+GGG | 0.519949 | 8.4:-44932058 | None:intergenic |
| TGCTCAGGGCAATAGAAAGA+AGG | 0.548493 | 8.4:+44932248 | MS.gene070472:CDS |
| CGCTGCACAAGAAAATTACA+AGG | 0.553852 | 8.4:+44931480 | MS.gene070472:CDS |
| GAGGTTCATCAACTTGCTCA+GGG | 0.558482 | 8.4:+44932234 | MS.gene070472:CDS |
| GATTAAAATCCAAGCATACT+GGG | 0.559851 | 8.4:-44931829 | None:intergenic |
| TGTATGATTTGCGAAACCAA+TGG | 0.592485 | 8.4:+44932626 | MS.gene070472:CDS |
| TCTACGTGTGCAGAGGACAC+TGG | 0.601997 | 8.4:+44932017 | MS.gene070472:intron |
| TAATATTACTAAGTCTTGGA+TGG | 0.604101 | 8.4:+44932464 | MS.gene070472:CDS |
| GATCTTGGTGCATTGAACAA+AGG | 0.604500 | 8.4:-44932081 | None:intergenic |
| AACATGAGCCAAATACCGCA+AGG | 0.641032 | 8.4:+44931581 | MS.gene070472:CDS |
| TATTACTAAGTCTTGGATGG+AGG | 0.643493 | 8.4:+44932467 | MS.gene070472:CDS |
| TTGCATCAACTCACGCTCGA+CGG | 0.688369 | 8.4:-44931335 | None:intergenic |
| TGCACAAGAAAATTACAAGG+AGG | 0.749080 | 8.4:+44931483 | MS.gene070472:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTAATCACTATATAAAAA+TGG | + | chr8.4:44932356-44932375 | MS.gene070472:intron | 15.0% |
| !! | AATAAGGATATGTTTATATT+TGG | - | chr8.4:44932534-44932553 | None:intergenic | 15.0% |
| !! | TATACTTACTTAAATAGATA+TGG | - | chr8.4:44931938-44931957 | None:intergenic | 15.0% |
| !!! | CTTTGATGATTTTTTTAATT+TGG | + | chr8.4:44932195-44932214 | MS.gene070472:intron | 15.0% |
| !!! | CTTTTTTACTATTATCAATA+TGG | + | chr8.4:44931695-44931714 | MS.gene070472:intron | 15.0% |
| !!! | TGTTAATTTTAAATTTGTCT+AGG | + | chr8.4:44931750-44931769 | MS.gene070472:intron | 15.0% |
| !! | AACACTTAAATATTGAATTG+TGG | - | chr8.4:44931734-44931753 | None:intergenic | 20.0% |
| ! | AATTTCTTCATTTGTTTGCA+AGG | - | chr8.4:44931404-44931423 | None:intergenic | 25.0% |
| ! | ACGTAGAAGAACAATTATTA+AGG | - | chr8.4:44932004-44932023 | None:intergenic | 25.0% |
| ! | GTTAATAATTATTTGTCCCA+AGG | + | chr8.4:44932042-44932061 | MS.gene070472:CDS | 25.0% |
| ! | TAATATTACTAAGTCTTGGA+TGG | + | chr8.4:44932464-44932483 | MS.gene070472:CDS | 25.0% |
| ! | TAGTACTAAAACTAAAGTTC+AGG | + | chr8.4:44931862-44931881 | MS.gene070472:CDS | 25.0% |
| ! | TATATTAACCTACACATTGT+AGG | + | chr8.4:44932567-44932586 | MS.gene070472:intron | 25.0% |
| !!! | GTTATACATTTTAGTGTTGT+AGG | - | chr8.4:44932176-44932195 | None:intergenic | 25.0% |
| !!! | GTTTTAGTACTATGATCTTT+TGG | - | chr8.4:44931854-44931873 | None:intergenic | 25.0% |
| AATCTACACCATACTTACTT+TGG | - | chr8.4:44932289-44932308 | None:intergenic | 30.0% | |
| ACAACTAAACTATTGTCCTT+GGG | - | chr8.4:44932061-44932080 | None:intergenic | 30.0% | |
| ATAAAAAACCTTGCGGTATT+TGG | - | chr8.4:44931592-44931611 | None:intergenic | 30.0% | |
| ATCTACACCATACTTACTTT+GGG | - | chr8.4:44932288-44932307 | None:intergenic | 30.0% | |
| ATTGAATACCTACAATGTGT+AGG | - | chr8.4:44932578-44932597 | None:intergenic | 30.0% | |
| CTGCTAATATTACTAAGTCT+TGG | + | chr8.4:44932460-44932479 | MS.gene070472:CDS | 30.0% | |
| CTGGATAAACTTAAGACTAT+GGG | + | chr8.4:44931367-44931386 | MS.gene070472:CDS | 30.0% | |
| GATTAAAATCCAAGCATACT+GGG | - | chr8.4:44931832-44931851 | None:intergenic | 30.0% | |
| TCATTGCTTCATTAAGATCT+TGG | - | chr8.4:44932099-44932118 | None:intergenic | 30.0% | |
| TCTGGATAAACTTAAGACTA+TGG | + | chr8.4:44931366-44931385 | MS.gene070472:CDS | 30.0% | |
| TGTATTAGATAGTTGTTGGT+GGG | + | chr8.4:44931618-44931637 | MS.gene070472:intron | 30.0% | |
| TGTTCAAAAATTGCTATGCA+TGG | + | chr8.4:44931522-44931541 | MS.gene070472:CDS | 30.0% | |
| TTCATTTGTTTGCAAGGTTT+GGG | - | chr8.4:44931398-44931417 | None:intergenic | 30.0% | |
| ! | CAATTTTTGAACATTGTCGA+TGG | - | chr8.4:44931515-44931534 | None:intergenic | 30.0% |
| !! | AGAAATAGATCGAAAGTGTT+TGG | + | chr8.4:44931797-44931816 | MS.gene070472:CDS | 30.0% |
| !! | GAAATAGATCGAAAGTGTTT+GGG | + | chr8.4:44931798-44931817 | MS.gene070472:CDS | 30.0% |
| ACCTGGAATAAAAAACCTTG+CGG | - | chr8.4:44931599-44931618 | None:intergenic | 35.0% | |
| CAACAACTATCTAATACACC+TGG | - | chr8.4:44931616-44931635 | None:intergenic | 35.0% | |
| CAGGTGTATTAGATAGTTGT+TGG | + | chr8.4:44931614-44931633 | MS.gene070472:intron | 35.0% | |
| CTTCATTTGTTTGCAAGGTT+TGG | - | chr8.4:44931399-44931418 | None:intergenic | 35.0% | |
| CTTTCTTGGCTACAAATGAA+TGG | - | chr8.4:44932382-44932401 | None:intergenic | 35.0% | |
| GACAACTAAACTATTGTCCT+TGG | - | chr8.4:44932062-44932081 | None:intergenic | 35.0% | |
| GCAATAGAAAGAAGGCTAAA+TGG | + | chr8.4:44932256-44932275 | MS.gene070472:CDS | 35.0% | |
| GGATTAAAATCCAAGCATAC+TGG | - | chr8.4:44931833-44931852 | None:intergenic | 35.0% | |
| GTGTATTAGATAGTTGTTGG+TGG | + | chr8.4:44931617-44931636 | MS.gene070472:intron | 35.0% | |
| TATCCACAATCATTGCCATT+AGG | - | chr8.4:44932408-44932427 | None:intergenic | 35.0% | |
| TATTACTAAGTCTTGGATGG+AGG | + | chr8.4:44932467-44932486 | MS.gene070472:CDS | 35.0% | |
| TGCACAAGAAAATTACAAGG+AGG | + | chr8.4:44931483-44931502 | MS.gene070472:CDS | 35.0% | |
| ! | CTTGTAATTTTCTTGTGCAG+CGG | - | chr8.4:44931482-44931501 | None:intergenic | 35.0% |
| ! | GTATGATTTGCGAAACCAAT+GGG | + | chr8.4:44932627-44932646 | MS.gene070472:CDS | 35.0% |
| ! | GTATTTGGCTCATGTTCTAA+TGG | - | chr8.4:44931577-44931596 | None:intergenic | 35.0% |
| ! | TGTATGATTTGCGAAACCAA+TGG | + | chr8.4:44932626-44932645 | MS.gene070472:CDS | 35.0% |
| !!! | ATCGAAAGTGTTTGGGATTT+TGG | + | chr8.4:44931805-44931824 | MS.gene070472:CDS | 35.0% |
| CAACCTAATGGCAATGATTG+TGG | + | chr8.4:44932402-44932421 | MS.gene070472:CDS | 40.0% | |
| CAAGAAAACAAGTTGCCCAT+TGG | - | chr8.4:44932645-44932664 | None:intergenic | 40.0% | |
| CCTGAAAAAGTTGTTGTGCA+TGG | + | chr8.4:44931436-44931455 | MS.gene070472:CDS | 40.0% | |
| CGCTGCACAAGAAAATTACA+AGG | + | chr8.4:44931480-44931499 | MS.gene070472:CDS | 40.0% | |
| GTTGATGCAACAAGCATTTC+TGG | + | chr8.4:44931348-44931367 | MS.gene070472:CDS | 40.0% | |
| GTTTCGTCCCAAAGTAAGTA+TGG | + | chr8.4:44932278-44932297 | MS.gene070472:intron | 40.0% | |
| TAGCCAAGAAAGCAACCTAA+TGG | + | chr8.4:44932390-44932409 | MS.gene070472:intron | 40.0% | |
| TCATCTTCAGTTAATGTCGC+TGG | - | chr8.4:44932604-44932623 | None:intergenic | 40.0% | |
| TTCAGGAACACATGCAAACT+AGG | + | chr8.4:44931879-44931898 | MS.gene070472:CDS | 40.0% | |
| ! | GATCTTGGTGCATTGAACAA+AGG | - | chr8.4:44932084-44932103 | None:intergenic | 40.0% |
| ! | TGGTTAACACAGAGCTTTAG+AGG | + | chr8.4:44932215-44932234 | MS.gene070472:intron | 40.0% |
| ! | TTGCCATTAGGTTGCTTTCT+TGG | - | chr8.4:44932396-44932415 | None:intergenic | 40.0% |
| !! | CCATGCACAACAACTTTTTC+AGG | - | chr8.4:44931439-44931458 | None:intergenic | 40.0% |
| !!! | ACCGCAAGGTTTTTTATTCC+AGG | + | chr8.4:44931595-44931614 | MS.gene070472:intron | 40.0% |
| AACATGAGCCAAATACCGCA+AGG | + | chr8.4:44931581-44931600 | MS.gene070472:CDS | 45.0% | |
| TGCTCAGGGCAATAGAAAGA+AGG | + | chr8.4:44932248-44932267 | MS.gene070472:CDS | 45.0% | |
| TTGTTCTTCTACGTGTGCAG+AGG | + | chr8.4:44932010-44932029 | MS.gene070472:intron | 45.0% | |
| ! | AGAGGTTCATCAACTTGCTC+AGG | + | chr8.4:44932233-44932252 | MS.gene070472:CDS | 45.0% |
| ! | AGTTGTTGGTGGGTATTGCT+AGG | + | chr8.4:44931628-44931647 | MS.gene070472:intron | 45.0% |
| ! | GAGGTTCATCAACTTGCTCA+GGG | + | chr8.4:44932234-44932253 | MS.gene070472:CDS | 45.0% |
| ! | GATTTTGGACCCAGTATGCT+TGG | + | chr8.4:44931820-44931839 | MS.gene070472:CDS | 45.0% |
| ! | GTTGTGCATGGAAGCACAAA+AGG | + | chr8.4:44931448-44931467 | MS.gene070472:CDS | 45.0% |
| !! | ATAAGTAATTAATATTAATA+AGG | - | chr8.4:44932550-44932569 | None:intergenic | 5.0% |
| TTGCATCAACTCACGCTCGA+CGG | - | chr8.4:44931338-44931357 | None:intergenic | 50.0% | |
| TCTACGTGTGCAGAGGACAC+TGG | + | chr8.4:44932017-44932036 | MS.gene070472:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 44931331 | 44932681 | 44931331 | ID=MS.gene070472 |
| chr8.4 | mRNA | 44931331 | 44932681 | 44931331 | ID=MS.gene070472.t1;Parent=MS.gene070472 |
| chr8.4 | exon | 44931331 | 44931602 | 44931331 | ID=MS.gene070472.t1.exon1;Parent=MS.gene070472.t1 |
| chr8.4 | CDS | 44931331 | 44931602 | 44931331 | ID=cds.MS.gene070472.t1;Parent=MS.gene070472.t1 |
| chr8.4 | exon | 44931772 | 44931948 | 44931772 | ID=MS.gene070472.t1.exon2;Parent=MS.gene070472.t1 |
| chr8.4 | CDS | 44931772 | 44931948 | 44931772 | ID=cds.MS.gene070472.t1;Parent=MS.gene070472.t1 |
| chr8.4 | exon | 44932030 | 44932134 | 44932030 | ID=MS.gene070472.t1.exon3;Parent=MS.gene070472.t1 |
| chr8.4 | CDS | 44932030 | 44932134 | 44932030 | ID=cds.MS.gene070472.t1;Parent=MS.gene070472.t1 |
| chr8.4 | exon | 44932227 | 44932290 | 44932227 | ID=MS.gene070472.t1.exon4;Parent=MS.gene070472.t1 |
| chr8.4 | CDS | 44932227 | 44932290 | 44932227 | ID=cds.MS.gene070472.t1;Parent=MS.gene070472.t1 |
| chr8.4 | exon | 44932393 | 44932488 | 44932393 | ID=MS.gene070472.t1.exon5;Parent=MS.gene070472.t1 |
| chr8.4 | CDS | 44932393 | 44932488 | 44932393 | ID=cds.MS.gene070472.t1;Parent=MS.gene070472.t1 |
| chr8.4 | exon | 44932589 | 44932681 | 44932589 | ID=MS.gene070472.t1.exon6;Parent=MS.gene070472.t1 |
| chr8.4 | CDS | 44932589 | 44932681 | 44932589 | ID=cds.MS.gene070472.t1;Parent=MS.gene070472.t1 |
| Gene Sequence |
| Protein sequence |
