Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070859.t1 | XP_024634430.1 | 95.6 | 91 | 3 | 1 | 1 | 91 | 248 | 337 | 8.40E-43 | 182.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070859.t1 | A0A3Q7YD19 | 74.7 | 91 | 22 | 1 | 1 | 91 | 104 | 193 | 1.1e-31 | 145.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052858 | MS.gene070859 | 0.8058 | 1.12E-49 | -1.69E-46 |
| MS.gene057140 | MS.gene070859 | 0.805492 | 1.30E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070859.t1 | MTR_2g017975 | 93.878 | 49 | 3 | 0 | 39 | 87 | 1 | 49 | 4.76e-26 | 95.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAACTTAACAAGGTTATC+TGG | 0.314955 | 6.4:-6297649 | MS.gene070859:CDS |
| CTGATGAGTTGTTTAGGATA+TGG | 0.326241 | 6.4:-6297744 | MS.gene070859:CDS |
| ATTTGCCTGATGAGTTGTTT+AGG | 0.395717 | 6.4:-6297750 | MS.gene070859:CDS |
| TCTTTGCCTTGAAGGTTTGT+AGG | 0.399596 | 6.4:+6297822 | None:intergenic |
| ACTATGAGTCTTTGCCTTGA+AGG | 0.404813 | 6.4:+6297814 | None:intergenic |
| CATTCGAGCTCATCCCTTCA+AGG | 0.405495 | 6.4:+6297674 | None:intergenic |
| GAGCTCATCCCTTCAAGGTT+AGG | 0.410036 | 6.4:+6297679 | None:intergenic |
| TGCCTTCCTACAAACCTTCA+AGG | 0.421938 | 6.4:-6297828 | MS.gene070859:CDS |
| GTTTAGGATATGGGAAGAAA+TGG | 0.432438 | 6.4:-6297734 | MS.gene070859:CDS |
| CATATCCTAAACAACTCATC+AGG | 0.463497 | 6.4:+6297745 | None:intergenic |
| TGATGAGTTGTTTAGGATAT+GGG | 0.463980 | 6.4:-6297743 | MS.gene070859:CDS |
| AACAAGGTTCCTAACCTTGA+AGG | 0.468744 | 6.4:-6297688 | MS.gene070859:CDS |
| ATAGGAATCATCTTAGAGAT+AGG | 0.479459 | 6.4:-6297600 | MS.gene070859:CDS |
| ATCATCTTAGAGATAGGATG+TGG | 0.498557 | 6.4:-6297594 | MS.gene070859:CDS |
| TGCCTTGAAGGTTTGTAGGA+AGG | 0.520257 | 6.4:+6297826 | None:intergenic |
| AGAACTTGAGGCGGTGAACA+AGG | 0.562806 | 6.4:-6297704 | MS.gene070859:CDS |
| TGGTGAAGGTGCAGCTGAGA+TGG | 0.570988 | 6.4:-6297629 | MS.gene070859:CDS |
| CAGCTGAGATGGCAATGGAT+AGG | 0.572746 | 6.4:-6297618 | MS.gene070859:CDS |
| CGAATGTTGAAAACTTAACA+AGG | 0.583345 | 6.4:-6297657 | MS.gene070859:CDS |
| AATGGATGATATAGAACTTG+AGG | 0.590391 | 6.4:-6297716 | MS.gene070859:CDS |
| TTAACAAGGTTATCTGGTGA+AGG | 0.596934 | 6.4:-6297643 | MS.gene070859:CDS |
| TTCACAATTACATACGCAAG+TGG | 0.598213 | 6.4:-6297774 | MS.gene070859:CDS |
| ACAAGGTTCCTAACCTTGAA+GGG | 0.611095 | 6.4:-6297687 | MS.gene070859:CDS |
| AGGTGCAGCTGAGATGGCAA+TGG | 0.645965 | 6.4:-6297623 | MS.gene070859:CDS |
| GGATGATATAGAACTTGAGG+CGG | 0.673217 | 6.4:-6297713 | MS.gene070859:CDS |
| TCATCTTAGAGATAGGATGT+GGG | 0.679475 | 6.4:-6297593 | MS.gene070859:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGGATGATATAGAACTTG+AGG | - | chr6.4:6297689-6297708 | MS.gene070859:CDS | 30.0% | |
| ATAGGAATCATCTTAGAGAT+AGG | - | chr6.4:6297805-6297824 | MS.gene070859:CDS | 30.0% | |
| CGAATGTTGAAAACTTAACA+AGG | - | chr6.4:6297748-6297767 | MS.gene070859:CDS | 30.0% | |
| GAAAACTTAACAAGGTTATC+TGG | - | chr6.4:6297756-6297775 | MS.gene070859:CDS | 30.0% | |
| ! | TGATGAGTTGTTTAGGATAT+GGG | - | chr6.4:6297662-6297681 | MS.gene070859:CDS | 30.0% |
| ATCATCTTAGAGATAGGATG+TGG | - | chr6.4:6297811-6297830 | MS.gene070859:CDS | 35.0% | |
| ATTTGCCTGATGAGTTGTTT+AGG | - | chr6.4:6297655-6297674 | MS.gene070859:CDS | 35.0% | |
| CATATCCTAAACAACTCATC+AGG | + | chr6.4:6297663-6297682 | None:intergenic | 35.0% | |
| TCATCTTAGAGATAGGATGT+GGG | - | chr6.4:6297812-6297831 | MS.gene070859:CDS | 35.0% | |
| TTAACAAGGTTATCTGGTGA+AGG | - | chr6.4:6297762-6297781 | MS.gene070859:CDS | 35.0% | |
| TTCACAATTACATACGCAAG+TGG | - | chr6.4:6297631-6297650 | MS.gene070859:CDS | 35.0% | |
| ! | CTGATGAGTTGTTTAGGATA+TGG | - | chr6.4:6297661-6297680 | MS.gene070859:CDS | 35.0% |
| ! | GTTTAGGATATGGGAAGAAA+TGG | - | chr6.4:6297671-6297690 | MS.gene070859:CDS | 35.0% |
| AACAAGGTTCCTAACCTTGA+AGG | - | chr6.4:6297717-6297736 | MS.gene070859:CDS | 40.0% | |
| ACAAGGTTCCTAACCTTGAA+GGG | - | chr6.4:6297718-6297737 | MS.gene070859:CDS | 40.0% | |
| ACTATGAGTCTTTGCCTTGA+AGG | + | chr6.4:6297594-6297613 | None:intergenic | 40.0% | |
| GGATGATATAGAACTTGAGG+CGG | - | chr6.4:6297692-6297711 | MS.gene070859:CDS | 40.0% | |
| TCTTTGCCTTGAAGGTTTGT+AGG | + | chr6.4:6297586-6297605 | None:intergenic | 40.0% | |
| TGCCTTCCTACAAACCTTCA+AGG | - | chr6.4:6297577-6297596 | MS.gene070859:CDS | 45.0% | |
| TGCCTTGAAGGTTTGTAGGA+AGG | + | chr6.4:6297582-6297601 | None:intergenic | 45.0% | |
| AGAACTTGAGGCGGTGAACA+AGG | - | chr6.4:6297701-6297720 | MS.gene070859:CDS | 50.0% | |
| CAGCTGAGATGGCAATGGAT+AGG | - | chr6.4:6297787-6297806 | MS.gene070859:CDS | 50.0% | |
| CATTCGAGCTCATCCCTTCA+AGG | + | chr6.4:6297734-6297753 | None:intergenic | 50.0% | |
| GAGCTCATCCCTTCAAGGTT+AGG | + | chr6.4:6297729-6297748 | None:intergenic | 50.0% | |
| AGGTGCAGCTGAGATGGCAA+TGG | - | chr6.4:6297782-6297801 | MS.gene070859:CDS | 55.0% | |
| ! | TGGTGAAGGTGCAGCTGAGA+TGG | - | chr6.4:6297776-6297795 | MS.gene070859:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 6297576 | 6297851 | 6297576 | ID=MS.gene070859 |
| chr6.4 | mRNA | 6297576 | 6297851 | 6297576 | ID=MS.gene070859.t1;Parent=MS.gene070859 |
| chr6.4 | exon | 6297576 | 6297851 | 6297576 | ID=MS.gene070859.t1.exon1;Parent=MS.gene070859.t1 |
| chr6.4 | CDS | 6297576 | 6297851 | 6297576 | ID=cds.MS.gene070859.t1;Parent=MS.gene070859.t1 |
| Gene Sequence |
| Protein sequence |
